FvH4_7g18411

Transcription elongation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0012966
Physical Location & Seq
Reverse (-)
15398387 .. 15408354
9968 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g18411.t2

Sequence Viewer

Length: 5100 bp
ATGGCCTCGAAGGATAAGGGGAAGGCGATCGCCGGGAGCGGATCGTCCGCCGCCGGCAAGCGCAAGCCCGGCGACGACAAGTCCGGCGGTGGGAGCCGGAAGCGGATGAACCCCGGCGTTCTCCAGTTCTTCGAGCACTCCGCCGCCGAAGCTGACGGCTCCGACGACAGCGACATGGACGATTTTATGGAAGAAGAACTTGAAGCAGAACCTATTGTCCAGAGTGAACCTGGGAAAGCCCGTAACCTTCCATTCATTCCTAAAGAGGAGGAAGTGGATGGGGAAGAATTTGAAAGAATGATGGAAGAACGATACAGGACTGGTTCTACATATGTTACCTATGCTGAAGATAATTATGAAAACAAAAGATCGATTGATGGAATTGTGCTTGAGCCTTCTGCTAAAGATCCTGTTGTCTGGAAAGTAAAGTGTGCGGTTGGACGCGAGAGACACTCAGCTTTCTGTATGATGCAGAAGTTTGTTGACTTAGCATCAATGGGTACCAAGCTGCAGATTATATCTGCATTTGCTGTTGATCACATCAAGGGTTTTATCTTCATTGAAGCTGATAAGCTATGTGACGTTCAGGAGGCATGTAAAGGACTTTGTAATATATTTCTATCCCGAGTAACGCCAGTTCCCAAAAGTGAAGCTCCTAATTTGTTGGCTCCTCGGACTAAAAACAGTGAAATTGCTGTCGGTACATGGGCCCGTGTGAAGAGTGGGAACTACAAAGGTGACTTGGGACAGGTTGTGGCTGTGAACGAAAAGAAAAAAGCAACAGTAAAGCTGATACCCAGAATTGATCTACAAGCAATGGCTATGAAATTTGGTGGGGGTGTTTCTAGAAAGAAACTGCCTACTCCAGCACCGAGATTGATCAGCACAAGCGAACTTGAGGAGTTCCGCCCTCTCATTCAGCACAGGAAGGATAAGGATACCGGATTGCATTTTCTCTGTTTTGATGGTTTGCTCCTTAAGGATGGATATTTATACAAAAAAGTGCCGTTAGATTCCTTAATTTGTCGGGGAGTCGTTCCATCAGATGAGGAGATACTGAAATTCAGGCCCTCCGAGAACAATGAATCTACTGATCTGGAGTGGCTTTCACAGCTCTATGGTGAAAATAAGAAAAGGAAGAGCGTCGACATTGATATAGGGGATGGCAAAGGAGAGGGTTCATCAAAGGGTAATGGGAAAGGAGAAGGTTCATCAAAGGGTAATGGGAAAGGAGAAGGTCCTTCAGGTGGGAAAAAAGGCTCCTCAGGGTGTACTGGGAAAGGAGAAAAAGAAGGCTCCTCAGGGTGTACTGGGAAAGGAGAAAGTTCTTCCGGGTGTGGTGAGAATCTCTATGGGATGTATGATCTTGTCTGTTTTGGCAAGAAAGATTTTGGTCTTGTGCTAGGCATTGAGAAAGATGATACTTACAAGATTCTGAAAGAGGGTTCGGAAGGATCTGCCGTTGTGACAATTCCACAGAAGGAGATAAAGAATGTACTCTCTGATGTAAAATTTACAGCTTATGATCAGCGCCAGAAGCCCATTGGTGTCAATGATACAGTTCAAGTTTTGGAAGGACCATTAAAGGATAGGCAAGGAATAGTGAAGCAAGTCTACAGAGGCACGATATTCATGTTTGATGAGAACGAGACAGAAAATGGTGGTTATTTCTGCTCAAAATCTCACATGTGTGAGAAAATCAAGCTTTCCATTGACGTCAGCCCAGAAAAGGATGGGGATTCGGGTGCTATGGACTTTGATGATTTCACATTATCACCCAAGTCCCCTCTATCACCAAAAAAGCCGTGGCTAAAGGAGAATAACTTCAATCAGGGCAATACTGACGGAATGTTCTCAATTGGTCAAACTTTAAGAATTCGTGTTGGTCCATTGAAGGGATACCTCTGTCGTGTCTTGGCCATACGCCGTGCTGATATCACTGTTAAGCTTGATTCTCAGCAAAGGGTTCTTACAGTTAAAGCTGAGCATCTTACAGAGGTTCGTGCAAAGAGCTCTGCCATGCTTAGTGAGGATCCGGAGTCCAGCTCTCTTAAACCATTCGATTTGCTTGGAACTGAAGGAGGCTCTACAGATTGGACCGATGGAGCTGGGACATCAGCCGGGGGTGATGCGTGGAATGCTGGAGGGTCATCTGGTGAAAGGAATGCTTGGCCTAGTTTCTCTGCCTCGGGCAATTCGCTTCAGCCTGAGTCCAGCTCTGCGAATCCTTTTGATTCTGATGGAAATGGTGCTAATAAAGAGGATAATTCTTGGGAGAGGAACATGGCTCCAAATAACAACTCAACCTGGGGTGTTGCAGCAGCAGAAAACAAGGATCAAGGTACTGGATGGGGAAAAAGCGAATCTTGGGGTGCGAAAGTTGGAGGTGACAGCAATCTGTCGGATACTTGGCAAAAAGCATCAGAACCCGCCAGTTCATCCTGGGGGGTTGCAGCAGCAGTAGATAACAAGGATGAAGGTACTGGTTGTGGAAAAAGCGACGCTTGGGGCGCTAAAGTTGGAGGTGACAGCACTTCGTCAGATACTTGGCAAAAAGCATCAGAACCTGCTAGTTCATCCTGGGGTGTAGCAGCAGCAGCAGATAACAAGGATCAAGGTTCTGTTTGGGGCGGTAATGATAGTCAGAAACCAAATGTTGCAGATGATAGTGGTGATCGAGGTTCTGCTTGGAGTAAGCCAGCTGGAGGTTCTTGGAGTAAACAAACTGGAGGATCCTCATGGGGTAAGCAAGAGGGCTCAAGTAATGGTGATGTAGGGTGGAAAAGTTCAATACCATCAGTAGAGAATCAGACTGGGAGCTGGGGTAATGCAGGTGGCTGGGCAAAAGCTCAAGGCGGATCTGGTAATGAGAATGAGAAAGACAGCTGGAAGAAGCCTAGTGGTGTAGAAGATGGAGGTTCTATCTGGAATAAACAAGATGGAGGTTCTACTTGGAATAATCCTGCTGGTGGTTCTTGGAGTAAAGGTGGGGATCAAGGGTCTGCTGGTGGGTATGGTGGCAATGGTGGTGGTGGTAGAGGGAGTGGAGGTGGGAATTGTTTTAAGTGTGGTGAGTCTGGACACATGTCCAGGGACTGCCCTCAGGGAGGCGGTGGTGGTAGAGGGAGTGGAGGTGGAAACTGTTTCAAATGTGGTGAGTCTGGCCACATGTCGAGGGAGTGCCCTCAGGGTGGCGGCGGTGGTAGAGGGGGTGGAAGTGGGAATTGTTTCAAGTGTGGTGAGTCGGGACACATGTCCAGGGAGTGCCCTCAGGGCGGCGGTGGTGGTAGAGGGGGTGGAAGTGGGAATTGTTTCAAGTGTGGTGAGTCGGGACACATGTCCAGGGAGTGCCCTCAGGGCGGCGGTGGTGGTAGAGGGGGTGGAGGTGGAAATTGTTTCAAGTGTGGTGAGTCAGGACACATGTCCAGGGAGTGCCCTCAGGGCGGCGGTGGTGGTAGAGGGAGTGGAGGTGGGAATTGTTTCAAGTGTGGTGAGCCTGGACACATGTCTAGGGAGTGCCCTCAGGGCGGCAGTGGTAGAGGCGCTGGAAGCGGGAACTGTTTCAAGTGTGGCGAGTCTGGACACATGGCTAGGGACTGCTCTCAGGGAGGTGGTAGCTATGGCGGCTTCGGTGGTGGAGGAAAGCCCACCGAGTCATGGAGCAAGAGTGCTTGGGGTAGTGGAGATGGTGGAAGTGGCAGCGGTGGAGCAAAGCCCACTGAATCATGGAGTAAGAGTGCTTGGGGTAGTGGAGACGGTGGAAGTGGCAGCGGTGAAGCAAAGCCCACCGAGTCATGGAGCAAGAGTGCTTGGGGTAGTGGAGATGGTGGAAGTGGCAGCGGTGGAGCAAAGCCCACTGAATCATGGAGTAAGAGTGCTTGGGGTAGTGGAGATGGTGGAAGTGGCAGCGGTGGAGCAAAGCCCACTGAATCATGGAGTAAGAGTGCTTGGGGTACTGGAGATGGTGGAAGTGGCAGCGGTGGAGCAAAGCCCACTGAATCATGGACTAAGAGTGCCTGGGGTTCTGGAGATGGTGGAAGTGGCAGTGGTGGTAGAGGGGGGAATTGTTACAAGTGTGGTGAATCTGGTCACATGTCTAGGGAGTGCCCTCAGGGCAGCGGTGGTGGTAGAGGGGGTGGAGGTGGTAATTGTTTCAAGTGCGGTGAATCTGGACATATGTCGAGGGAGTGTCCTCAGGGGGGCGGTGGTGGTAGAGGGGCTGGAGGTGGTAACTGTTTCAAGTGCGGTGAATCTGGACATATGTCAAGGGAGTGTCCTCAGGGGGGCGGTGGTGGTAGAGGGGCTGGAGGTGGTAACTGTTTTAAGTGCGGTGAATCTGGTCACATGTCGAGGGAGTGCCCTCAGGGCGGTGGTGGTGGGGGTAGAGGGGCTGGAGGTGGGAACTGTTACAAATGTGGTGAGTCTGGACACATGTCTAGGGAGTGCCCTCAGGGCGGCAGTGGTAGAGGCGCTGGAAGCGGGAACTGTTTCAAGTGTGGTGAGTCTGGACACATGGCTAGGGACTGCTCTCAGGGAGGTGGTAGCTATGGTGGCTTCGGTGGTGGAGCGAAGCCCACCGAGTCATGGAGCAAGAGTGCTTGGGGTAGTGGTGGAGATGGTGGAAGTGGCAGTGGTGGAGCAAAGCCCACTGAATCATGGAGCAAGAGTGCCTGGGGTACTGGAGATGGTGGAAGTGGCATTTTGGGAGCAAAACCAGTATCAAGCTCTTGGGGTGCCGCAGCAGATGGCCGAAGTGGCGGAGCTACTGGTGAAGCAAAGCCTGGTGATTCATGGAGCAAGCCGTCAGCAAGTGCGTGGGGTAAGGGAGATGGTGGAAGTGGCGGAGCTACTGGTGAGCCAAAGCCTGCTGATTCATGGAGCAAGCCATCAGCAAGTGCCTGGGGTAATGGAGATGGTGCAAGCGGTGGAGCTAGTAGTGAAGCCAAGCCTGCTGATTCATGGACCAAACCATCAGCTAGCGCCTGGGGTAATGGAGATGGTGGAAATGTTGGAGCTAGTGAGGAAGCAAAGCCCGCTGATGCATGGAGCAAGCCGTCATCAAGTGCTTGGGGAAGTGGAAGTGGCTCCGGAGGGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006351 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006368 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0006996 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016043 GO:0016070 GO:0016071 GO:0016246 GO:0016441 GO:0016458 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0030422 GO:0031047 GO:0031050 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032044 GO:0032259 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044728 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0071840 GO:0080090 GO:0080188 GO:0090304 GO:0097159 GO:0097659 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901698 GO:1901699 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Spt5-NGN

Protein Analysis

1700

Amino Acids

172.57

Weight (kDa)

6.31

Isoelectric Point (pI)

39.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Spt5-NGN PF03439 139 - 222 6.6e-19 Early transcription elongation factor of RNA pol II, NGN section
KOW1_SPT5 PF23042 232 - 355 1.5e-26 Transcription elongation factor SPT5, KOW1 domain
KOWx_SPT5 PF23037 451 - 498 9.4e-08 Transcription elongation factor SPT5, KOWx domain
KOW4_SPT5 PF23291 507 - 561 2.2e-21 Transcription elongation factor SPT5, fourth KOW domain
KOW5_SPT5 PF23290 620 - 664 3.9e-06 Transcription elongation factor SPT5, fifth KOW domain
zf-CCHC PF00098 1019 - 1035 7.9e-07 Zinc knuckle
zf-CCHC PF00098 1047 - 1063 2e-06 Zinc knuckle
zf-CCHC PF00098 1075 - 1091 2e-06 Zinc knuckle
zf-CCHC PF00098 1103 - 1119 2e-06 Zinc knuckle
zf-CCHC PF00098 1131 - 1147 2e-06 Zinc knuckle
zf-CCHC PF00098 1208 - 1224 1e-06 Zinc knuckle
zf-CCHC PF00098 1236 - 1252 2e-06 Zinc knuckle
zf-CCHC PF00098 1264 - 1280 2e-06 Zinc knuckle
zf-CCHC PF00098 1292 - 1308 2e-06 Zinc knuckle
zf-CCHC PF00098 1321 - 1337 1e-06 Zinc knuckle
zf-CCHC PF00098 1348 - 1364 2.7e-06 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2822
AatII GACGTC 1 cut(s) 1719
Acc36I ACCTGC 2 cut(s) 2575, 2822
Acc65I GGTACC 1 cut(s) 500
AccB1I GGYRCC 2 cut(s) 500, 4733
AccBSI CCGCTC 1 cut(s) 39
AccI GTMKAC 2 cut(s) 1146, 1614
AccII CGCG 1 cut(s) 444
AccIII TCCGGA 2 cut(s) 2035, 5087
AcoI YGGCCR 3 cut(s) 1917, 3163, 4747
AcsI RAATTY 5 cut(s) 287, 827, 1061, 1511, 1875
AcuI CTGAAG 4 cut(s) 366, 1227, 2097, 2186
AcyI GRCGYC 1 cut(s) 1716
AfaI GTAC 9 cut(s) 502, 703, 1273, 1309, 1497, 2344, 2482, 3955, 4678
AflII CTTAAG 1 cut(s) 977
AhdI GACNNNNNGTC 1 cut(s) 79
AleI CACNNNNGTG 1 cut(s) 1689
AloI GAACNNNNNNTCC 2 cut(s) 201, 233
Alw21I GWGCWC 2 cut(s) 138, 2015
Alw26I GTCTC 3 cut(s) 442, 1643, 3747
AlwNI CAGNNNCTG 2 cut(s) 2567, 3096
Ama87I CYCGRG 2 cut(s) 624, 2188
Aor13HI TCCGGA 2 cut(s) 2035, 5087
AoxI GGCC 7 cut(s) 3, 708, 1067, 1917, 2171, 3163, 4747
ApaI GGGCCC 1 cut(s) 712
ApoI RAATTY 5 cut(s) 287, 827, 1061, 1511, 1875
AsiSI GCGATCGC 1 cut(s) 30
Asp700I GAANNNNTTC 6 cut(s) 1823, 3227, 3311, 3479, 3560, 4487
Asp718I GGTACC 1 cut(s) 500
AspLEI GCGC 6 cut(s) 63, 1533, 2513, 3545, 4472, 4982
AspS9I GGNCC 8 cut(s) 708, 709, 1068, 1238, 1577, 1886, 2097, 4962
AsuC2I CCSGG 5 cut(s) 34, 69, 114, 1333, 2122
AsuNHI GCTAGC 1 cut(s) 4976
AvaI CYCGRG 2 cut(s) 624, 2188
AvaII GGWCC 5 cut(s) 1238, 1577, 1886, 2097, 4962
BaeGI GKGCMC 9 cut(s) 712, 3185, 3269, 3353, 3437, 3521, 4109, 4361, 4448
BaeI ACNNNNGTAYC 2 cut(s) 1891, 1924
BalI TGGCCA 2 cut(s) 1919, 3165
BamHI GGATCC 2 cut(s) 2032, 2732
BanI GGYRCC 2 cut(s) 500, 4733
BanII GRGCYC 3 cut(s) 712, 2015, 2759
BarI GAAGNNNNNNTAC 2 cut(s) 1598, 1630
Bbv12I GWGCWC 2 cut(s) 138, 2015
BceAI ACGGC 7 cut(s) 172, 991, 1445, 1789, 1911, 4786, 5038
BciVI GTATCC 3 cut(s) 931, 1892, 2398
BclI TGATCA 3 cut(s) 535, 879, 1525
BcnI CCSGG 5 cut(s) 34, 69, 114, 1333, 2122
BcoDI GTCTC 3 cut(s) 442, 1643, 3747
BfmI CTRYAG 3 cut(s) 509, 1615, 2088
BfoI RGCGCY 5 cut(s) 1534, 2514, 3546, 4473, 4983
BfrI CTTAAG 1 cut(s) 977
BfuAI ACCTGC 2 cut(s) 2575, 2822
BfuI GTATCC 3 cut(s) 931, 1892, 2398
BglI GCCNNNNNGGC 8 cut(s) 3273, 3357, 3441, 3525, 4113, 4365, 4452, 4755
BlpI GCTNAGC 1 cut(s) 1983
Bme18I GGWCC 5 cut(s) 1238, 1577, 1886, 2097, 4962
BmeRI GACNNNNNGTC 1 cut(s) 79
BmeT110I CYCGRG 2 cut(s) 624, 2188
BmgT120I GGNCC 8 cut(s) 708, 709, 1068, 1238, 1577, 1886, 2097, 4962
BmrI ACTGGG 3 cut(s) 1284, 1320, 2823
BmsI GCATC 7 cut(s) 459, 500, 1996, 2119, 2429, 2567, 5029
BmtI GCTAGC 1 cut(s) 4980
BmuI ACTGGG 3 cut(s) 1284, 1320, 2823
BoxI GACNNNNGTC 8 cut(s) 3085, 3253, 3337, 3421, 3505, 4177, 4261, 4432
BplI GAGNNNNNCTC 6 cut(s) 437, 469, 2030, 2062, 2201, 2233
Bpu1102I GCTNAGC 1 cut(s) 1983
BpuEI CTTGAG 4 cut(s) 410, 917, 2743, 2835
BpuMI CCSGG 5 cut(s) 34, 69, 114, 1333, 2122
Bsa29I ATCGAT 1 cut(s) 371
BsaHI GRCGYC 1 cut(s) 1716
BsaWI WCCGGW 3 cut(s) 941, 2035, 5087
Bse118I RCCGGY 1 cut(s) 53
Bse3DI GCAATG 2 cut(s) 822, 3028
BseAI TCCGGA 2 cut(s) 2035, 5087
BseCI ATCGAT 1 cut(s) 371
BseMI GCAATG 2 cut(s) 822, 3028
BseRI GAGGAG 6 cut(s) 281, 660, 914, 1064, 1252, 1288
BseSI GKGCMC 9 cut(s) 712, 3185, 3269, 3353, 3437, 3521, 4109, 4361, 4448
BseYI CCCAGC 3 cut(s) 2108, 2820, 2838
Bsh1236I CGCG 1 cut(s) 444
Bsh1285I CGRYCG 1 cut(s) 30
BshFI GGCC 7 cut(s) 5, 710, 1069, 1919, 2173, 3165, 4749
BshNI GGYRCC 2 cut(s) 500, 4733
BshVI ATCGAT 1 cut(s) 371
BsiEI CGRYCG 1 cut(s) 30
BsiHKAI GWGCWC 2 cut(s) 138, 2015
BsiHKCI CYCGRG 2 cut(s) 624, 2188
BslFI GGGAC 8 cut(s) 759, 1768, 2125, 3107, 3261, 3345, 3608, 4535
BsmAI GTCTC 3 cut(s) 442, 1643, 3747
BsmBI CGTCTC 1 cut(s) 3747
BsmFI GGGAC 8 cut(s) 759, 1768, 2125, 3107, 3261, 3345, 3608, 4535
BsmI GAATGC 2 cut(s) 2143, 2170
BsnI GGCC 7 cut(s) 5, 710, 1069, 1919, 2173, 3165, 4749
BsoBI CYCGRG 2 cut(s) 624, 2188
Bsp120I GGGCCC 1 cut(s) 708
Bsp13I TCCGGA 2 cut(s) 2035, 5087
Bsp1720I GCTNAGC 1 cut(s) 1983
BspANI GGCC 7 cut(s) 5, 710, 1069, 1919, 2173, 3165, 4749
BspDI ATCGAT 1 cut(s) 371
BspEI TCCGGA 2 cut(s) 2035, 5087
BspFNI CGCG 1 cut(s) 444
BspMAI CTGCAG 1 cut(s) 513
BspMI ACCTGC 2 cut(s) 2575, 2822
BspOI GCTAGC 1 cut(s) 4980
BspQI GCTCTTC 1 cut(s) 1133
BspT107I GGYRCC 2 cut(s) 500, 4733
BspTI CTTAAG 1 cut(s) 977
BsrBI CCGCTC 1 cut(s) 39
BsrDI GCAATG 2 cut(s) 822, 3028
BsrFI RCCGGY 1 cut(s) 53
BssAI RCCGGY 1 cut(s) 53
BssNI GRCGYC 1 cut(s) 1716
Bst6I CTCTTC 2 cut(s) 713, 1133
BstACI GRCGYC 1 cut(s) 1716
BstAFI CTTAAG 1 cut(s) 977
BstDSI CCRYGG 1 cut(s) 1805
BstENI CCTNNNNNAGG 1 cut(s) 3105
BstFNI CGCG 1 cut(s) 444
BstH2I RGCGCY 5 cut(s) 1534, 2514, 3546, 4473, 4983
BstHHI GCGC 6 cut(s) 63, 1533, 2513, 3545, 4472, 4982
BstMAI GTCTC 3 cut(s) 442, 1643, 3747
BstMCI CGRYCG 1 cut(s) 30
BstPAI GACNNNNGTC 8 cut(s) 3085, 3253, 3337, 3421, 3505, 4177, 4261, 4432
BstSFI CTRYAG 3 cut(s) 509, 1615, 2088
BstSLI GKGCMC 9 cut(s) 712, 3185, 3269, 3353, 3437, 3521, 4109, 4361, 4448
BstUI CGCG 1 cut(s) 444
BstX2I RGATCY 5 cut(s) 406, 1454, 2032, 2732, 2858
BstYI RGATCY 5 cut(s) 406, 1454, 2032, 2732, 2858
Bsu15I ATCGAT 1 cut(s) 371
BsuI GTATCC 3 cut(s) 931, 1892, 2398
BsuRI GGCC 7 cut(s) 5, 710, 1069, 1919, 2173, 3165, 4749
BsuTUI ATCGAT 1 cut(s) 371
BtgI CCRYGG 1 cut(s) 1805
BtsI GCAGTG 4 cut(s) 3538, 4051, 4465, 4636
BveI ACCTGC 2 cut(s) 2575, 2822
CaiI CAGNNNCTG 2 cut(s) 2567, 3096
CfoI GCGC 6 cut(s) 63, 1533, 2513, 3545, 4472, 4982
Cfr10I RCCGGY 1 cut(s) 53
Cfr13I GGNCC 8 cut(s) 708, 709, 1068, 1238, 1577, 1886, 2097, 4962
ClaI ATCGAT 1 cut(s) 371
CseI GACGC 3 cut(s) 450, 1132, 2510
Csp6I GTAC 9 cut(s) 501, 702, 1272, 1308, 1496, 2343, 2481, 3954, 4677
CspCI CAANNNNNGTGG 2 cut(s) 3010, 3045
CviQI GTAC 9 cut(s) 501, 702, 1272, 1308, 1496, 2343, 2481, 3954, 4677
DriI GACNNNNNGTC 1 cut(s) 79
EaeI YGGCCR 3 cut(s) 1917, 3163, 4747
Eam1104I CTCTTC 2 cut(s) 713, 1133
Eam1105I GACNNNNNGTC 1 cut(s) 79
EarI CTCTTC 2 cut(s) 713, 1133
EciI GGCGGA 6 cut(s) 37, 130, 896, 2871, 4773, 4857
Ecl136II GAGCTC 1 cut(s) 2013
Eco24I GRGCYC 3 cut(s) 712, 2015, 2759
Eco32I GATATC 1 cut(s) 1936
Eco47I GGWCC 5 cut(s) 1238, 1577, 1886, 2097, 4962
Eco53kI GAGCTC 1 cut(s) 2013
Eco57I CTGAAG 4 cut(s) 366, 1227, 2097, 2186
Eco88I CYCGRG 2 cut(s) 624, 2188
EcoICRI GAGCTC 1 cut(s) 2013
EcoNI CCTNNNNNAGG 1 cut(s) 3105
EcoO109I RGGNCCY 2 cut(s) 1068, 1238
EcoRI GAATTC 1 cut(s) 1875
EcoRV GATATC 1 cut(s) 1936
EcoT22I ATGCAT 1 cut(s) 5044
EcoT38I GRGCYC 3 cut(s) 712, 2015, 2759
Esp3I CGTCTC 1 cut(s) 3747
FalI AAGNNNNNCTT 8 cut(s) 183, 215, 2152, 2184, 2350, 2382, 2488, 2520
FaqI GGGAC 8 cut(s) 759, 1768, 2125, 3107, 3261, 3345, 3608, 4535
FauI CCCGC 4 cut(s) 2437, 3545, 4472, 5041
FauNDI CATATG 3 cut(s) 331, 4176, 4260
FbaI TGATCA 3 cut(s) 535, 879, 1525
FblI GTMKAC 2 cut(s) 1146, 1614
FriOI GRGCYC 3 cut(s) 712, 2015, 2759
GlaI GCGC 6 cut(s) 62, 1532, 2512, 3544, 4471, 4981
GsaI CCCAGC 3 cut(s) 2112, 2824, 2842
HaeII RGCGCY 5 cut(s) 1534, 2514, 3546, 4473, 4983
HaeIII GGCC 7 cut(s) 5, 710, 1069, 1919, 2173, 3165, 4749
HgaI GACGC 3 cut(s) 450, 1132, 2510
HhaI GCGC 6 cut(s) 63, 1533, 2513, 3545, 4472, 4982
Hin1I GRCGYC 1 cut(s) 1716
Hin6I GCGC 6 cut(s) 61, 1531, 2511, 3543, 4470, 4980
HinP1I GCGC 6 cut(s) 61, 1531, 2511, 3543, 4470, 4980
HincII GTYRAC 2 cut(s) 484, 1147
HindII GTYRAC 2 cut(s) 484, 1147
HindIII AAGCTT 2 cut(s) 1703, 1946
Hpy166II GTNNAC 8 cut(s) 227, 484, 763, 1147, 1272, 1308, 1615, 2720
Hpy8I GTNNAC 8 cut(s) 227, 484, 763, 1147, 1272, 1308, 1615, 2720
Hpy99I CGWCG 4 cut(s) 77, 167, 1148, 2504
HpyCH4IV ACGT 2 cut(s) 582, 1716
HpySE526I ACGT 2 cut(s) 582, 1716
Hsp92I GRCGYC 1 cut(s) 1716
HspAI GCGC 6 cut(s) 61, 1531, 2511, 3543, 4470, 4980
Kpn2I TCCGGA 2 cut(s) 2035, 5087
KpnI GGTACC 1 cut(s) 504
KroI GCCGGC 1 cut(s) 53
KroNI GCCGGC 1 cut(s) 55
Ksp22I TGATCA 3 cut(s) 535, 879, 1525
LguI GCTCTTC 1 cut(s) 1133
LweI GCATC 7 cut(s) 459, 500, 1996, 2119, 2429, 2567, 5029
MaeII ACGT 2 cut(s) 582, 1716
MbiI CCGCTC 1 cut(s) 39
MfeI CAATTG 1 cut(s) 1857
MflI RGATCY 5 cut(s) 406, 1454, 2032, 2732, 2858
MlsI TGGCCA 2 cut(s) 1919, 3165
MluNI TGGCCA 2 cut(s) 1919, 3165
MmeI TCCRAC 6 cut(s) 186, 418, 2362, 2382, 2500, 4990
Mox20I TGGCCA 2 cut(s) 1919, 3165
Mph1103I ATGCAT 1 cut(s) 5044
MroI TCCGGA 2 cut(s) 2035, 5087
MroNI GCCGGC 1 cut(s) 53
MroXI GAANNNNTTC 6 cut(s) 1823, 3227, 3311, 3479, 3560, 4487
MscI TGGCCA 2 cut(s) 1919, 3165
MseI TTAA 9 cut(s) 978, 1019, 1583, 1871, 1944, 1977, 2052, 3063, 4323
MslI CAYNNNNRTG 1 cut(s) 1689
Msp20I TGGCCA 2 cut(s) 1919, 3165
MspA1I CMGCKG 9 cut(s) 2702, 2886, 3702, 3771, 3840, 3909, 3978, 4119, 5036
MspCI CTTAAG 1 cut(s) 977
MunI CAATTG 1 cut(s) 1857
Mva1269I GAATGC 2 cut(s) 2143, 2170
MvnI CGCG 1 cut(s) 444
NaeI GCCGGC 1 cut(s) 55
NciI CCSGG 5 cut(s) 34, 69, 114, 1333, 2122
NdeI CATATG 3 cut(s) 331, 4176, 4260
NgoMIV GCCGGC 1 cut(s) 53
NheI GCTAGC 1 cut(s) 4976
NmuCI GTSAC 7 cut(s) 578, 737, 1465, 2387, 2525, 4088, 4340
NsiI ATGCAT 1 cut(s) 5044
OliI CACNNNNGTG 1 cut(s) 1689
PaqCI CACCTGC 1 cut(s) 2822
PciSI GCTCTTC 1 cut(s) 1133
PcsI WCGNNNNNNNCGW 1 cut(s) 162
PctI GAATGC 2 cut(s) 2143, 2170
PdiI GCCGGC 1 cut(s) 55
PdmI GAANNNNTTC 6 cut(s) 1823, 3227, 3311, 3479, 3560, 4487
Ple19I CGATCG 1 cut(s) 30
PpuMI RGGWCCY 1 cut(s) 1238
PshAI GACNNNNGTC 8 cut(s) 3085, 3253, 3337, 3421, 3505, 4177, 4261, 4432
Psp124BI GAGCTC 1 cut(s) 2015
Psp5II RGGWCCY 1 cut(s) 1238
PspFI CCCAGC 3 cut(s) 2108, 2820, 2838
PspOMI GGGCCC 1 cut(s) 708
PspPI GGNCC 8 cut(s) 708, 709, 1068, 1238, 1577, 1886, 2097, 4962
PspPPI RGGWCCY 1 cut(s) 1238
PsrI GAACNNNNNNTAC 2 cut(s) 621, 653
PstI CTGCAG 1 cut(s) 513
PstNI CAGNNNCTG 2 cut(s) 2567, 3096
PsuI RGATCY 5 cut(s) 406, 1454, 2032, 2732, 2858
PvuI CGATCG 1 cut(s) 30
PvuII CAGCTG 2 cut(s) 2702, 2886
RgaI GCGATCGC 1 cut(s) 30
RsaI GTAC 9 cut(s) 502, 703, 1273, 1309, 1497, 2344, 2482, 3955, 4678
RsaNI GTAC 9 cut(s) 501, 702, 1272, 1308, 1496, 2343, 2481, 3954, 4677
RseI CAYNNNNRTG 1 cut(s) 1689
SacI GAGCTC 1 cut(s) 2015
SalI GTCGAC 1 cut(s) 1145
SapI GCTCTTC 1 cut(s) 1133
SaqAI TTAA 9 cut(s) 978, 1019, 1583, 1871, 1944, 1977, 2052, 3063, 4323
Sau96I GGNCC 8 cut(s) 708, 709, 1068, 1238, 1577, 1886, 2097, 4962
SfaAI GCGATCGC 1 cut(s) 30
SfaNI GCATC 7 cut(s) 459, 500, 1996, 2119, 2429, 2567, 5029
SfcI CTRYAG 3 cut(s) 509, 1615, 2088
SgfI GCGATCGC 1 cut(s) 30
SinI GGWCC 5 cut(s) 1238, 1577, 1886, 2097, 4962
SmiMI CAYNNNNRTG 1 cut(s) 1689
SmlI CTYRAG 5 cut(s) 389, 896, 977, 2758, 2850
SmoI CTYRAG 5 cut(s) 389, 896, 977, 2758, 2850
SstI GAGCTC 1 cut(s) 2015
TaiI ACGT 2 cut(s) 585, 1719
TaqI TCGA 9 cut(s) 8, 132, 371, 1146, 2061, 2677, 3173, 4181, 4349
TaqII GACCGA 1 cut(s) 2114
TatI WGTACW 3 cut(s) 1271, 1307, 1495
Tru1I TTAA 9 cut(s) 978, 1019, 1583, 1871, 1944, 1977, 2052, 3063, 4323
Tru9I TTAA 9 cut(s) 978, 1019, 1583, 1871, 1944, 1977, 2052, 3063, 4323
TseFI GTSAC 7 cut(s) 578, 737, 1465, 2387, 2525, 4088, 4340
Tsp45I GTSAC 7 cut(s) 578, 737, 1465, 2387, 2525, 4088, 4340
TspGWI ACGGA 1 cut(s) 1860
Vha464I CTTAAG 1 cut(s) 977
VpaK11BI GGWCC 5 cut(s) 1238, 1577, 1886, 2097, 4962
XagI CCTNNNNNAGG 1 cut(s) 3105
XapI RAATTY 5 cut(s) 287, 827, 1061, 1511, 1875
XbaI TCTAGA 1 cut(s) 845
XcmI CCANNNNNNNNNTGG 3 cut(s) 227, 1541, 1803
XmiI GTMKAC 2 cut(s) 1146, 1614
XmnI GAANNNNTTC 6 cut(s) 1823, 3227, 3311, 3479, 3560, 4487
ZraI GACGTC 1 cut(s) 1717
Zsp2I ATGCAT 1 cut(s) 5044
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.