Rroxscaffold_4G00294410

Transcription elongation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0012966
Physical Location & Seq
Forward (+)
14487203 .. 14500215
13013 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00294410.1

Sequence Viewer

Length: 4296 bp
ATGGGTACCAAGCTGCAGATAATATCTGCATTTGCAGTTGATCACATCAAGGGTTTTGTTTTCATTGAAGCTGATAAGCTATGTGACGTTCAGGAGGCATGTAAAGGACTTTGTAATATATTTTTATCCCGAGTAACGCCAGTTCCCAAAAATGAAGCTCCTCATTTACTGGCTCCTCGGACTAGAAACAGTGAAATTGCTGTGGGTACATGGGCCCGTGTGAAGAGTGGGACCTACAAGGGTGACTTGGCACAGGTTGTGGCTGTGAACGAAAAGAAAAAAGCAACAGTAAAGCTGATACCAAGAATTGATCTACAAGCAATGGCTGCAAAATTTGGTGGGGGAGTTACTAGAAAGAAACTGCCTACTCCAGCACCGAGATTGATCAGCACAAGCGAACTTGAGGAGTTCCGCCCTCTCATTCAGGACAGGAAGGATAAGGATACCGGATTGCGTTTTCTCTGTCTTGATGGTTTGCTCCTTAAGGATGGATATTTATACAAAAAGATGCCATTAGATTCATTAAATTGCTGGGGTATCATGCCATCAGAAGAGGAGATACTGAAATTCAAGCCTTCTGAGAACAATGAATCTGCTGATCTGGAGTGGCTTTCACAGCTTTATGGTGACAATAAGAAAAGGAAGAGCATCGGCAGTGATAAAGGGGATGGCAAAGGAGAAGGTTCAGCAGGGTGTGGTGGGAAAGGAGAAGGTTCATCAAAGGGTAACGGGAAAGGAGGTCCTTCAGGTGGGAAAGAAGGTTCTTCAGGGTTTACTGGGAAAGGAGAAGGTTCTTCAGGGTGTGGTGGGAATCTCTATGAGCTGTATGATCTTGTTTGTTTCGGCAAGAAAGATTTTGGTCTTGTGCTAGGCTTTGAGAAAGATGATACTTACAAGATTCTGAAAGAGGGTGTGGAAGGATCTACTGTTGTGTCAATTCAGCAGAATGAGATAAAGAATGTACTTTCTGATGTCAAATTTACAGCTTTAGATCAGTGCCTGAAGTCCATTCGCGTCAATGATACAGTTAAAGTTTTGGAAGGACCATTGAAGGATAGGCAAGGAATAGTGAAGCAAGTCTACAGAGGCACAATATTCATGTTTGATGAGAATGAGACAGAAAATGGTGGTTATTTCTGCTCAAAATCTCAAATGTGTGAGAAAATCAAGCTTTCCATTGACGTTATCCCAGAAAAGGATGGGGATTCGGGTGCTTTCGGTTTTGAAGATTTCACATCATCACCTAAATCACCTCTATCGCCAAAAAAGCCTTGGCTAGGAAGGGAGAATAACTTCAATCAGGGCGATACTGACGGAATGTTCTCAATCGGTCAAACTTTAAGAATTCGTGTTGGTCCATTAAAGGGATACCTCTGTCGTGTCTTGGCCATACGCCGTTCTGATATCACAGTTAAGCTTGATTCTCAGCAAAGGGTGCTTACAGTTAAAGCTGAGCATCTTACAGAGGTTCGTGTAAAGAACGCTGCCATGCTTAGTGAGGATCCGGAGTCCAGCTCTCTTAAACCATTTGATTTACTTGGAACTGAAGGAGGCTCTAAAGATTGGACAGATGGAGCTGGGACATCAGCCGGGGGTGATGGGTGGAATGCTGGAGGGTCATCTGGTGAAAGGAATGCTTGGCCTAGTTTCTCTGCCTCAGGCAATTCGCTTCAGCCTGAGTCCAGCTCTGCGAATCCTTTTGAATCTGATGGAAATGGTGCTAAAAAAGAGGATAGTTCTTGGGAGAGGAACGTGGCTCCAAATAACAACTCATCCTGGGGTGTTGCAGCAGCAGAAAACAAGGATCAAGTTACTGGCTGGGGAAAAAGCAACTCTTGGGGCGCTAAAGTTGGAGATGACAGCACTTCGTCGGATACTTGGCAAAAAGCATCAGAACCTGCCAGTTCATCCTGGGGTGTTGCAGCAGCTGTAAATAACAAGGATCAAGGTACCGGCTGGGGAAAAAGCGATTCTTGGGGCGCTAAAGTTGGAGGTGACAGCAGTCTGTCAGATACTTTGCAAAAAGCATCAGAACCTGCCAGTTCATCCTGGGGTGTTGCAGCAGCAGTAGATAACAAGGATCAAGGTACTGGCTGGGGAAAAAGTGACTCTTGGGGTGCTAAAGTTGGAGGTGACAGCACTTCGTCGGATACTTGGCAAAAAGCATCAGCGCCTGCTAGTTCATCCTGGGGTGTTGTGGCAGCAATAGATAAGAAGGATCAAAGTACTGGCTGGGGTGCTAAGACTGGATGTGACGGCACTGCATCAGATAGTTGGAAAAAAGCATCAGAACCTGCTAGTAATTCGTCCTGGGGTGTTGCAGCAGCAGCAGATAACAAGGATCAAGGTTCCGTCTGGGGCGGTAATGGTAGTCAAAAACCAAGTGTTGCAGATGATAGTGGTGACAAGGGTTCTGCTTGGAATAAGCCAGCTGAAGGTTCTTGGAGTAAACAAACTGGAGGATCCTCATGGGGTAAGCAAGCCGATTGGAGCACTAAAGAGGGATCAAGTACTGGTGATGCAGGGTGGAAAAGTTCAACGCCTTCAGTAGAGAATCAGACTGGGAGCTGGGGTAATGCAGGTGGCTGGGCAAAAGCTCAAGGCATATCTGGTAATGAGAATCATAAAGACAGTTGGAAGAAACCAAGTGGTATAGATGACAATAAAAATGCTTCGTGGATCAAGGAAGATGGAGGTTCTACCTGGAATAAACAAGATGGAGGTTCTACTTGGAATAAGCCTGCTGGAGGTTCTTGGAGTAAAGGTGGGGATCAAGGGTCTGGTGGTGGCTATGGTGGCGATGGTGGTGGTGGTAGAGGGGGTGGAGGTGGGAACTGTTTTAAGTGCGGTGAGTCTGGACACATGTCTAGGGAGTGCCCTCAGGGCGGCAGTGGTGGTAGAGGGACTGGAGGTGGGAACTGTTTCAAGTGCGGTGAGTCTGGACACATGTCTAGGGAGTGCCCTCAGGGTGGCGGTGGTGGTAGAGGGGGTGGAGGTGGGAATTGTTTCAAATGCGGTGAGTCTGGACACATGTCTAGGGAGTGCCCTCAGGGTGGTGGTGGTGGTGGTAGAGGGGGGAACTGTTACAAGTGTGGTGAATCTGGACACATGTCTAGGGAGTGCCCTCAGGGCGGAGGTGGTGGTAGAGGGGGCGGAGGTGGTAACTGTTTCAAGTGCGGTGAATCTGGACATATGTCAAGGGAGTGCCCTCAGGGTGGCGGTGGTGGTAGAGGGGCAGGAGGTGGTAACTGTTTCAAGTGCGGTGAGTCTGGACATATGTCAAGGGAGTGCCCTCAGGGCGGCAGTGGTGGTAGAGGGGCTGGAGGTGGGAACTGTTACAAGTGTGGTGAGTCTGGACACATGTCTAGGGAGTGCCCTCAGGGCGGCGGTGGTGGTAGAGGGAGTGGGGGTGGGAACTGTTTCAAATGTGGTGAGTCTGGACACATGTCTAGGGAGTGTCCTCAGGGCGGCGGTGGTAGAGGGGCTGGAAGTGGGAACTGTTTCAAGTGTGGTGAGTCTGGACACATGGCTAGGGACTGCTATCAGGGAGGTGGTAGCTATGGCAACTTTGGTGGTGGAGCAAGGTCCACTGATTCATGGAGCAAGCGTGCTTGGGGTACTGGAGATGGTGGAAGTGGCGGTGATGGAGCAAAACCAGCAGCAAGCTCTTGGGGTACTGGAGATGGTGGAAGTGGCTTTGGTGGTGGAGCAAAGCCCACTGATTCATGGAGCAAGCGTGCTTGGGGTACTGGAGATGGTGGAAGTGGCAGCGGTGGACCAAAACCAGCAGGAAGCTCTTGGGGTACAAATGGTGGAAGTGGCGGAGCTGGTGGTGAAGCAAAACCAGCAGCAAGCTCTTGGGGTAAGGGAGATGGTGGAAGTGGCAGAGCTAGTGATGAAGCAAAACCAGCAGCAAGCTCTTGGGGTAAGGCAGATGGTGGAAGTGGCAGAGCTAGTGGTGAAGCAAAACCAGCAGCAAGCACTTGGGGTACAGGAAGCTCTTGGGGTAAGGGAGATGGTGGAAGTGGCGGAGCTGGTGGTGAAGCAAAGCCTGCTGATCCATGGAGCAAGCCATCAGCAAGTGCTTGGGGTAAAGGAGATGGTGGAAGTGGCGGAGCTAATGGTGAAGCAAAGCCTGGTGATCTATGGAGCAAGCCATCAGCAAGTGCTTGGGGTAAGAAAGATGGTGGAAGTGTTGGAGCTAGTGGTGAAGCAAAGCCTGCTGATCCATGGAGCAAGCCATCAGCAAGTGCTTGGGGTAAGGGAGATGGTGGAAGTGGCAGCGGAGGGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006351 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006368 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0006996 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016043 GO:0016070 GO:0016071 GO:0016246 GO:0016441 GO:0016458 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0030422 GO:0031047 GO:0031050 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032044 GO:0032259 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044728 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0071840 GO:0080090 GO:0080188 GO:0090304 GO:0097159 GO:0097659 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901698 GO:1901699 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Spt5-NGN

Protein Analysis

1431

Amino Acids

145.29

Weight (kDa)

8.17

Isoelectric Point (pI)

37.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Spt5-NGN PF03439 3 - 57 1.6e-10 Early transcription elongation factor of RNA pol II, NGN section
KOW1_SPT5 PF23042 67 - 190 3.4e-26 Transcription elongation factor SPT5, KOW1 domain
KOWx_SPT5 PF23037 270 - 320 1.3e-08 Transcription elongation factor SPT5, KOWx domain
KOW4_SPT5 PF23291 329 - 383 7.1e-22 Transcription elongation factor SPT5, fourth KOW domain
KOW5_SPT5 PF23290 443 - 487 1.8e-06 Transcription elongation factor SPT5, fifth KOW domain
zf-CCHC PF00098 945 - 961 1.8e-06 Zinc knuckle
zf-CCHC PF00098 973 - 989 1.8e-06 Zinc knuckle
zf-CCHC PF00098 1001 - 1017 1.8e-06 Zinc knuckle
zf-CCHC PF00098 1027 - 1043 9.4e-07 Zinc knuckle
zf-CCHC PF00098 1055 - 1071 1.8e-06 Zinc knuckle
zf-CCHC PF00098 1083 - 1099 1.8e-06 Zinc knuckle
zf-CCHC PF00098 1111 - 1127 9.4e-07 Zinc knuckle
zf-CCHC PF00098 1139 - 1155 1.8e-06 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2570
Acc36I ACCTGC 4 cut(s) 1906, 2044, 2302, 2570
Acc65I GGTACC 2 cut(s) 5, 1949
AccB1I GGYRCC 2 cut(s) 5, 1949
AccI GTMKAC 1 cut(s) 1080
AccII CGCG 1 cut(s) 1014
AccIII TCCGGA 1 cut(s) 1504
AcoI YGGCCR 1 cut(s) 1386
AcsI RAATTY 4 cut(s) 332, 566, 977, 1344
AcuI CTGAAG 8 cut(s) 729, 750, 780, 1022, 1566, 1655, 2454, 2529
AflII CTTAAG 1 cut(s) 482
AflIII ACRYGT 6 cut(s) 2862, 2946, 3030, 3108, 3360, 3444
AjnI CCWGG 7 cut(s) 1775, 1910, 2048, 2186, 2309, 2702, 4135
AloI GAACNNNNNNTCC 2 cut(s) 745, 777
Alw21I GWGCWC 1 cut(s) 2495
Alw26I GTCTC 1 cut(s) 1109
AlwNI CAGNNNCTG 6 cut(s) 1000, 1898, 1928, 2036, 2174, 2294
Ama87I CYCGRG 1 cut(s) 129
Aor13HI TCCGGA 1 cut(s) 1504
AoxI GGCC 3 cut(s) 213, 1386, 1640
ApaI GGGCCC 1 cut(s) 217
ApoI RAATTY 4 cut(s) 332, 566, 977, 1344
Asp700I GAANNNNTTC 5 cut(s) 1292, 2921, 3005, 3419, 3500
Asp718I GGTACC 2 cut(s) 5, 1949
AspLEI GCGC 3 cut(s) 1844, 1982, 2173
AspS9I GGNCC 8 cut(s) 213, 214, 231, 740, 1043, 1355, 3585, 3776
AsuC2I CCSGG 1 cut(s) 1591
AvaI CYCGRG 1 cut(s) 129
AvaII GGWCC 6 cut(s) 231, 740, 1043, 1355, 3585, 3776
AxyI CCTNAGG 9 cut(s) 1657, 2880, 2964, 3048, 3126, 3210, 3294, 3378, 3462
BaeGI GKGCMC 8 cut(s) 217, 2879, 2963, 3047, 3125, 3209, 3293, 3377
BaeI ACNNNNGTAYC 2 cut(s) 1360, 1393
BalI TGGCCA 1 cut(s) 1388
BamHI GGATCC 2 cut(s) 1501, 2462
BanI GGYRCC 2 cut(s) 5, 1949
BanII GRGCYC 1 cut(s) 217
BarI GAAGNNNNNNTAC 4 cut(s) 543, 575, 1064, 1096
Bbv12I GWGCWC 1 cut(s) 2495
BceAI ACGGC 2 cut(s) 1380, 2272
BciT130I CCWGG 7 cut(s) 1777, 1912, 2050, 2188, 2311, 2704, 4137
BciVI GTATCC 4 cut(s) 436, 1361, 1867, 2143
BclI TGATCA 2 cut(s) 40, 384
BcnI CCSGG 1 cut(s) 1591
BcoDI GTCTC 1 cut(s) 1109
BfmI CTRYAG 2 cut(s) 14, 1081
BfoI RGCGCY 3 cut(s) 1845, 1983, 2174
BfrI CTTAAG 1 cut(s) 482
BfuAI ACCTGC 4 cut(s) 1906, 2044, 2302, 2570
BfuI GTATCC 4 cut(s) 436, 1361, 1867, 2143
BglI GCCNNNNNGGC 4 cut(s) 2883, 3129, 3297, 3381
BlpI GCTNAGC 1 cut(s) 1452
BmcAI AGTACT 2 cut(s) 2227, 2512
Bme1390I CCNGG 8 cut(s) 1591, 1777, 1912, 2050, 2188, 2311, 2704, 4137
Bme18I GGWCC 6 cut(s) 231, 740, 1043, 1355, 3585, 3776
BmeT110I CYCGRG 1 cut(s) 129
BmgT120I GGNCC 8 cut(s) 213, 214, 231, 740, 1043, 1355, 3585, 3776
BmiI GGNNCC 9 cut(s) 7, 174, 215, 232, 1503, 1758, 1951, 2350, 2464
BmrFI CCNGG 8 cut(s) 1591, 1777, 1912, 2050, 2188, 2311, 2704, 4137
BmrI ACTGGG 2 cut(s) 786, 2571
BmsI GCATC 9 cut(s) 498, 657, 1465, 1898, 2036, 2174, 2273, 2294, 2509
BmuI ACTGGG 2 cut(s) 786, 2571
BoxI GACNNNNGTC 9 cut(s) 2001, 2863, 2947, 3031, 3109, 3193, 3277, 3361, 3445
BplI GAGNNNNNCTC 4 cut(s) 1499, 1531, 1670, 1702
Bpu1102I GCTNAGC 1 cut(s) 1452
BpuEI CTTGAG 2 cut(s) 422, 2583
BpuMI CCSGG 1 cut(s) 1591
BsaWI WCCGGW 2 cut(s) 446, 1504
Bse118I RCCGGY 1 cut(s) 1952
Bse21I CCTNAGG 9 cut(s) 1657, 2880, 2964, 3048, 3126, 3210, 3294, 3378, 3462
Bse3DI GCAATG 1 cut(s) 327
BseAI TCCGGA 1 cut(s) 1504
BseBI CCWGG 7 cut(s) 1777, 1912, 2050, 2188, 2311, 2704, 4137
BseGI GGATG 8 cut(s) 493, 673, 1204, 1772, 1907, 2045, 2183, 2255
BseMI GCAATG 1 cut(s) 327
BseRI GAGGAG 4 cut(s) 150, 165, 419, 569
BseSI GKGCMC 8 cut(s) 217, 2879, 2963, 3047, 3125, 3209, 3293, 3377
BseYI CCCAGC 8 cut(s) 531, 1577, 1818, 1956, 2094, 2232, 2568, 2586
Bsh1236I CGCG 1 cut(s) 1014
BshFI GGCC 3 cut(s) 215, 1388, 1642
BshNI GGYRCC 2 cut(s) 5, 1949
BsiHKAI GWGCWC 1 cut(s) 2495
BsiHKCI CYCGRG 1 cut(s) 129
BsiSI CCGG 4 cut(s) 447, 1505, 1590, 1953
BslFI GGGAC 4 cut(s) 244, 1594, 2917, 3548
BsmAI GTCTC 1 cut(s) 1109
BsmFI GGGAC 4 cut(s) 244, 1594, 2917, 3548
BsmI GAATGC 2 cut(s) 1612, 1639
BsnI GGCC 3 cut(s) 215, 1388, 1642
BsoBI CYCGRG 1 cut(s) 129
Bsp120I GGGCCC 1 cut(s) 213
Bsp1286I GDGCHC 9 cut(s) 217, 2495, 2879, 2963, 3047, 3125, 3209, 3293, 3377
Bsp13I TCCGGA 1 cut(s) 1504
Bsp1720I GCTNAGC 1 cut(s) 1452
Bsp19I CCATGG 2 cut(s) 4061, 4229
BspANI GGCC 3 cut(s) 215, 1388, 1642
BspEI TCCGGA 1 cut(s) 1504
BspFNI CGCG 1 cut(s) 1014
BspLI GGNNCC 9 cut(s) 7, 174, 215, 232, 1503, 1758, 1951, 2350, 2464
BspMAI CTGCAG 1 cut(s) 18
BspMI ACCTGC 4 cut(s) 1906, 2044, 2302, 2570
BspQI GCTCTTC 1 cut(s) 638
BspT107I GGYRCC 2 cut(s) 5, 1949
BspTI CTTAAG 1 cut(s) 482
BsrDI GCAATG 1 cut(s) 327
BsrFI RCCGGY 1 cut(s) 1952
BssAI RCCGGY 1 cut(s) 1952
BssT1I CCWWGG 3 cut(s) 1271, 4061, 4229
Bst2UI CCWGG 7 cut(s) 1777, 1912, 2050, 2188, 2311, 2704, 4137
Bst6I CTCTTC 3 cut(s) 218, 546, 638
BstAFI CTTAAG 1 cut(s) 482
BstAPI GCANNNNNTGC 4 cut(s) 326, 1435, 4052, 4220
BstDSI CCRYGG 2 cut(s) 4061, 4229
BstENI CCTNNNNNAGG 2 cut(s) 1275, 2745
BstF5I GGATG 8 cut(s) 493, 673, 1204, 1772, 1907, 2045, 2183, 2255
BstFNI CGCG 1 cut(s) 1014
BstH2I RGCGCY 3 cut(s) 1845, 1983, 2174
BstHHI GCGC 3 cut(s) 1844, 1982, 2173
BstMAI GTCTC 1 cut(s) 1109
BstNI CCWGG 7 cut(s) 1777, 1912, 2050, 2188, 2311, 2704, 4137
BstNSI RCATGY 7 cut(s) 102, 2866, 2950, 3034, 3112, 3364, 3448
BstPAI GACNNNNGTC 9 cut(s) 2001, 2863, 2947, 3031, 3109, 3193, 3277, 3361, 3445
BstSCI CCNGG 8 cut(s) 1589, 1775, 1910, 2048, 2186, 2309, 2702, 4135
BstSFI CTRYAG 2 cut(s) 14, 1081
BstSLI GKGCMC 8 cut(s) 217, 2879, 2963, 3047, 3125, 3209, 3293, 3377
BstUI CGCG 1 cut(s) 1014
BstX2I RGATCY 3 cut(s) 920, 1501, 2462
BstYI RGATCY 3 cut(s) 920, 1501, 2462
Bsu36I CCTNAGG 9 cut(s) 1657, 2880, 2964, 3048, 3126, 3210, 3294, 3378, 3462
BsuI GTATCC 4 cut(s) 436, 1361, 1867, 2143
BsuRI GGCC 3 cut(s) 215, 1388, 1642
BtgI CCRYGG 2 cut(s) 4061, 4229
BtgZI GCGATG 1 cut(s) 2814
BtsCI GGATG 8 cut(s) 493, 673, 1204, 1772, 1907, 2045, 2183, 2255
BtsI GCAGTG 4 cut(s) 661, 2259, 2896, 3310
BtsIMutI CAGTG 8 cut(s) 196, 661, 1001, 2259, 2896, 3310, 3588, 3717
BveI ACCTGC 4 cut(s) 1906, 2044, 2302, 2570
CaiI CAGNNNCTG 6 cut(s) 1000, 1898, 1928, 2036, 2174, 2294
CfoI GCGC 3 cut(s) 1844, 1982, 2173
Cfr10I RCCGGY 1 cut(s) 1952
Cfr13I GGNCC 8 cut(s) 213, 214, 231, 740, 1043, 1355, 3585, 3776
CseI GACGC 1 cut(s) 1003
CspCI CAANNNNNGTGG 2 cut(s) 3553, 3588
EaeI YGGCCR 1 cut(s) 1386
Eam1104I CTCTTC 3 cut(s) 218, 546, 638
EarI CTCTTC 3 cut(s) 218, 546, 638
EciI GGCGGA 6 cut(s) 401, 3147, 3168, 3837, 4044, 4128
Eco130I CCWWGG 3 cut(s) 1271, 4061, 4229
Eco24I GRGCYC 1 cut(s) 217
Eco32I GATATC 1 cut(s) 1405
Eco47I GGWCC 6 cut(s) 231, 740, 1043, 1355, 3585, 3776
Eco57I CTGAAG 8 cut(s) 729, 750, 780, 1022, 1566, 1655, 2454, 2529
Eco81I CCTNAGG 9 cut(s) 1657, 2880, 2964, 3048, 3126, 3210, 3294, 3378, 3462
Eco88I CYCGRG 1 cut(s) 129
EcoNI CCTNNNNNAGG 2 cut(s) 1275, 2745
EcoO109I RGGNCCY 2 cut(s) 231, 740
EcoRI GAATTC 1 cut(s) 1344
EcoRII CCWGG 7 cut(s) 1775, 1910, 2048, 2186, 2309, 2702, 4135
EcoRV GATATC 1 cut(s) 1405
EcoT14I CCWWGG 3 cut(s) 1271, 4061, 4229
EcoT38I GRGCYC 1 cut(s) 217
ErhI CCWWGG 3 cut(s) 1271, 4061, 4229
FaqI GGGAC 4 cut(s) 244, 1594, 2917, 3548
FauNDI CATATG 2 cut(s) 3192, 3276
FbaI TGATCA 2 cut(s) 40, 384
FblI GTMKAC 1 cut(s) 1080
FokI GGATG 8 cut(s) 500, 680, 1211, 1759, 1894, 2032, 2170, 2262
FriOI GRGCYC 1 cut(s) 217
GlaI GCGC 3 cut(s) 1843, 1981, 2172
GsaI CCCAGC 8 cut(s) 535, 1581, 1822, 1960, 2098, 2236, 2572, 2590
HaeII RGCGCY 3 cut(s) 1845, 1983, 2174
HaeIII GGCC 3 cut(s) 215, 1388, 1642
HapII CCGG 4 cut(s) 447, 1505, 1590, 1953
HgaI GACGC 1 cut(s) 1003
HhaI GCGC 3 cut(s) 1844, 1982, 2173
Hin6I GCGC 3 cut(s) 1842, 1980, 2171
HinP1I GCGC 3 cut(s) 1842, 1980, 2171
HindIII AAGCTT 2 cut(s) 1169, 1415
HpaII CCGG 4 cut(s) 447, 1505, 1590, 1953
Hpy166II GTNNAC 6 cut(s) 268, 774, 1081, 2450, 3588, 3776
Hpy8I GTNNAC 6 cut(s) 268, 774, 1081, 2450, 3588, 3776
Hpy99I CGWCG 2 cut(s) 1873, 2149
HpyCH4IV ACGT 3 cut(s) 87, 1182, 1752
HpySE526I ACGT 3 cut(s) 87, 1182, 1752
HspAI GCGC 3 cut(s) 1842, 1980, 2171
Kpn2I TCCGGA 1 cut(s) 1504
KpnI GGTACC 2 cut(s) 9, 1953
Ksp22I TGATCA 2 cut(s) 40, 384
LguI GCTCTTC 1 cut(s) 638
LweI GCATC 9 cut(s) 498, 657, 1465, 1898, 2036, 2174, 2273, 2294, 2509
MaeII ACGT 3 cut(s) 87, 1182, 1752
MboII GAAGA 8 cut(s) 235, 563, 655, 756, 786, 1238, 2650, 2699
MflI RGATCY 3 cut(s) 920, 1501, 2462
MhlI GDGCHC 9 cut(s) 217, 2495, 2879, 2963, 3047, 3125, 3209, 3293, 3377
MlsI TGGCCA 1 cut(s) 1388
MluNI TGGCCA 1 cut(s) 1388
MmeI TCCRAC 8 cut(s) 1831, 1851, 1969, 2107, 2127, 2255, 2615, 4177
Mox20I TGGCCA 1 cut(s) 1388
MroI TCCGGA 1 cut(s) 1504
MroXI GAANNNNTTC 5 cut(s) 1292, 2921, 3005, 3419, 3500
MscI TGGCCA 1 cut(s) 1388
MseI TTAA 9 cut(s) 483, 524, 1029, 1340, 1361, 1413, 1446, 1521, 2841
Msp20I TGGCCA 1 cut(s) 1388
MspA1I CMGCKG 4 cut(s) 1928, 2432, 3771, 4284
MspCI CTTAAG 1 cut(s) 482
MspI CCGG 4 cut(s) 447, 1505, 1590, 1953
MspR9I CCNGG 8 cut(s) 1591, 1777, 1912, 2050, 2188, 2311, 2704, 4137
Mva1269I GAATGC 2 cut(s) 1612, 1639
MvaI CCWGG 7 cut(s) 1777, 1912, 2050, 2188, 2311, 2704, 4137
MvnI CGCG 1 cut(s) 1014
NciI CCSGG 1 cut(s) 1591
NcoI CCATGG 2 cut(s) 4061, 4229
NdeI CATATG 2 cut(s) 3192, 3276
NlaIV GGNNCC 9 cut(s) 7, 174, 215, 232, 1503, 1758, 1951, 2350, 2464
NmuCI GTSAC 8 cut(s) 83, 242, 626, 1994, 2105, 2132, 2252, 2402
NspI RCATGY 7 cut(s) 102, 2866, 2950, 3034, 3112, 3364, 3448
PaqCI CACCTGC 1 cut(s) 2570
PciI ACATGT 6 cut(s) 2862, 2946, 3030, 3108, 3360, 3444
PciSI GCTCTTC 1 cut(s) 638
PctI GAATGC 2 cut(s) 1612, 1639
PdmI GAANNNNTTC 5 cut(s) 1292, 2921, 3005, 3419, 3500
PpuMI RGGWCCY 2 cut(s) 231, 740
PscI ACATGT 6 cut(s) 2862, 2946, 3030, 3108, 3360, 3444
PshAI GACNNNNGTC 9 cut(s) 2001, 2863, 2947, 3031, 3109, 3193, 3277, 3361, 3445
Psp5II RGGWCCY 2 cut(s) 231, 740
Psp6I CCWGG 7 cut(s) 1775, 1910, 2048, 2186, 2309, 2702, 4135
PspFI CCCAGC 8 cut(s) 531, 1577, 1818, 1956, 2094, 2232, 2568, 2586
PspGI CCWGG 7 cut(s) 1775, 1910, 2048, 2186, 2309, 2702, 4135
PspN4I GGNNCC 9 cut(s) 7, 174, 215, 232, 1503, 1758, 1951, 2350, 2464
PspOMI GGGCCC 1 cut(s) 213
PspPI GGNCC 8 cut(s) 213, 214, 231, 740, 1043, 1355, 3585, 3776
PspPPI RGGWCCY 2 cut(s) 231, 740
PsrI GAACNNNNNNTAC 2 cut(s) 126, 158
PstI CTGCAG 1 cut(s) 18
PstNI CAGNNNCTG 6 cut(s) 1000, 1898, 1928, 2036, 2174, 2294
PsuI RGATCY 3 cut(s) 920, 1501, 2462
PvuII CAGCTG 2 cut(s) 1928, 2432
SapI GCTCTTC 1 cut(s) 638
SaqAI TTAA 9 cut(s) 483, 524, 1029, 1340, 1361, 1413, 1446, 1521, 2841
Sau96I GGNCC 8 cut(s) 213, 214, 231, 740, 1043, 1355, 3585, 3776
ScaI AGTACT 2 cut(s) 2227, 2512
ScrFI CCNGG 8 cut(s) 1591, 1777, 1912, 2050, 2188, 2311, 2704, 4137
SduI GDGCHC 9 cut(s) 217, 2495, 2879, 2963, 3047, 3125, 3209, 3293, 3377
SfaNI GCATC 9 cut(s) 498, 657, 1465, 1898, 2036, 2174, 2273, 2294, 2509
SfcI CTRYAG 2 cut(s) 14, 1081
SinI GGWCC 6 cut(s) 231, 740, 1043, 1355, 3585, 3776
SmlI CTYRAG 3 cut(s) 401, 482, 2598
SmoI CTYRAG 3 cut(s) 401, 482, 2598
SspI AATATT 1 cut(s) 1095
StyD4I CCNGG 8 cut(s) 1589, 1775, 1910, 2048, 2186, 2309, 2702, 4135
StyI CCWWGG 3 cut(s) 1271, 4061, 4229
TaiI ACGT 3 cut(s) 90, 1185, 1755
TaqII GACCGA 1 cut(s) 1319
TatI WGTACW 3 cut(s) 961, 2225, 2510
TauI GCSGC 4 cut(s) 2889, 3303, 3387, 3471
Tru1I TTAA 9 cut(s) 483, 524, 1029, 1340, 1361, 1413, 1446, 1521, 2841
Tru9I TTAA 9 cut(s) 483, 524, 1029, 1340, 1361, 1413, 1446, 1521, 2841
TscAI CASTG 8 cut(s) 196, 661, 1001, 2266, 2896, 3310, 3595, 3724
TseFI GTSAC 8 cut(s) 83, 242, 626, 1994, 2105, 2132, 2252, 2402
Tsp45I GTSAC 8 cut(s) 83, 242, 626, 1994, 2105, 2132, 2252, 2402
TspGWI ACGGA 2 cut(s) 1329, 2341
TspRI CASTG 8 cut(s) 196, 661, 1001, 2266, 2896, 3310, 3595, 3724
Vha464I CTTAAG 1 cut(s) 482
VpaK11BI GGWCC 6 cut(s) 231, 740, 1043, 1355, 3585, 3776
XagI CCTNNNNNAGG 2 cut(s) 1275, 2745
XapI RAATTY 4 cut(s) 332, 566, 977, 1344
XceI RCATGY 7 cut(s) 102, 2866, 2950, 3034, 3112, 3364, 3448
XcmI CCANNNNNNNNNTGG 1 cut(s) 1269
XmiI GTMKAC 1 cut(s) 1080
XmnI GAANNNNTTC 5 cut(s) 1292, 2921, 3005, 3419, 3500
ZrmI AGTACT 2 cut(s) 2227, 2512
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.