RLG00000027664

Transcription elongation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0012966
Physical Location & Seq
Forward (+)
13697829 .. 13707681
9853 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027664

Sequence Viewer

Length: 5016 bp
ATGGCCTCAAAGGAAAAAGGAAAGGCGATCGCCACAAGCGGATCGTCCGCCGCAGGCAAGCGGAAGCCGGGCGATGACAAGTCCGGCGGTGGCAGTCGGAAGCGGAGAAACCCCGGCGTTCTCCAGTTCTTCGAGCACTCCGCCGCCGAAGCTGACGGTTCGGACGACGACAGCGACTTGGACGATTTTATGGAAGAAGAACTTGAAACAGAACCTGTAGTCAAGAGTGAACTGGGGAAAGCCCATAACCTTCCATTTGTTCCCAAAGAGGAGGATATCGATGGGGAAGAATTTGAAAGAATGATGGAAGAACGATACAGGACTGGTTCTACATATGTTACCTATGCTGAAGATAATTATGAAAACAAAAGATCGGTTGATGGAATTGTGCTTGAGCCTTCTGCTAAAGATCCTGTTATCTGGAAAGTAAAGTGTGCGGTTGGACGCGAGAGACACTCAGCTTTCTGTATGATGCAGAAGTTTGTTGACTTACGATCAATGGGTACCAAGCTGCAGATAATATCTGCATTTGCTGTTGATCACATCAAGGGTTTTGTTTTCATTGAAGCTGATAAGCTATGTGACGTTCAGGAGGCATGTAAAGGACTTTGTAATATATTTTTATCCCGAGTGACGCCAGTTCCCAAAAATGAAGCTCCTCATTTACTGGCTCCTCGGACTAGAAACAGTGAAATTGCTGTGGGTACATGGGCCCGTGTGAAGAGTGGGAACTACAAAGGTGACTTGGCACAGGTTGTGGCTGTGAACGAAAAGAAAAAAGCAACAGTAAAGCTGATACCAAGAATTGATCTACAAGCAATGGCTGCAAAATTTGGTGGGGGAGTTACTAGAAAGAAACTGCCTACTCCAGCACCGAGATTGATCAGCACAAGCGAACTTGAGGAGTTCCGCCCTCTCATTCAGGACAGGAAGGATAAGGATACCGGATTGCGTTTTCTCTGTCTTGATGGTTTGCTCCTTAAGGATGGATATTTATACAAAAAAATGCCATTAGATTCATTAAATTGCGGGGGTGTCATGCCCTCAGAAGAGGAGATACTGAAATTCAAGCCTTCTGAGAACAATGAATCTGCTGATCTGGAGTGGCTTTCACAGCTTTATGGTGACAATAAGAAAAGGAAGAGCATCGGCAGTGATAAGGGGAATGGCAAAGGAGAAGGTTCAGCAGGGTGTGGTGGGAAAGGAGAAGGGTCATCAAAGGGTAATGGGAAAGGAGAAGGTCCTTCAGGTGGGAAAGAAGGTTCTTCAGGGTTTACTGGGAAAGGAGAAGGTTCTTCAGGGTGTGGTGGGAATCTCTATGAGCTGTATGATCTTGTTTGTTTCGGCAAGAAAGATTTTGGTCTGGTGCTAGGCATTGAGAAAGATGATACTTACAAGATTCTGAAAGAGGGTGCGGAAGGATCTACTGTTGTGACAGTTCAGCAGAATGAGATAAAGAATGTACTTTCTGATGTAAAATTTACAGCTTTAGATCAGCGCCTGAAGCCCATTTGCGTCAATGATACAGTTAAAGTTTTGGAAGGACCATTGAAGGATAGGCAAGGAATAGTGAAGCAAGTCTACAGAGGCACAATATTCATGTTTGATGAGAATGAGACAGAAAATGGTGGTTATTTCTGCTCAAAATCTCAAATGTGTGAGAAAATCAAGCTTTCCATTGACGTTATCCTAGGAAAGGATGGGGATGCAGGTGCTTTCGGTTTTGAAGATTTCACATTATCACCTAAATCCCCTCTATCGCCGAAAAAGCCATGGCTAGGAAGGGAGAATAACTTCAATCAGGGCGATACTGACGGAATGTTCTCAATCGGTCAAACTTTAAGAATTCGTGTTGGTCCATTAAAGGGATACCTCTGTCGTGTCTTGGCCATACGCCGTTCTGATATCACAGTTAAGCTTGATTCTCAGCAAAGGGTTCTTACAGTTAAAGCTGAGCATCTTACAGAGGTTCGTGTAAAGAGCGCTGCCATGCTTAGTGAGGATCCGGAGTCCAGCTCTCTTAAACCATTTGATTTACTTGGAACTGAAGGAGGCTCGAAAGATTGGACAGATGGAGCTGGGACATCAGCCGGGGGTGATGGGTGGAATGCTGGAGGATCATCTGGTGAAGGGAATGCTTGGCCTAGTTTCTCTGCCTCGGGCAATTCGCTTCAGCCTGAGTCCAGCTCTGCGAATCCTTTTGAATCTGATGGAAATGGTGCTAAAAAAGAGGATAGTTCTTGGGAGAGGAACGTGGCTCCAAATAACAACTCATCCTGGGGTGTTGCAGCAGCAGAAAACAAGGATCAAGTTACTGGCTGGGGAAAAAGCAACTCTTGGGGCGCTAAAGTTGGAGATGACAGCACTTCGTCGGATACTTGGCAAAAAGCATCAGAACCTGCCAGTTCATCCTGGGGTGTTGCAGCAGCTGTAAATAACAAGGATCAAGGTACTGGCTGGGGAAAAAGCGATTCTTGGGGCGCTAAAGTTGGAGGTGACAGCAGTCTGTCAGATACTTTGCAACAAGCATCAGAACCTGCCAGTTCATCCTGGGGTGTTGTAGCAGCAGTAGATAACAAGGATCAAGGTACTGGCTGGCAAAAAAGCGACTCTTGGGGTGCTAAAGTTGGAGGTGACAGCACTTCATCGGATACTTGGCAAAAAGCATCAGCGCCTGCTAGTTCATCCTGGGGTGTTGCGGCAGCAATAGATAAGAAGGATCAAGGTACTGGCTGGGGTGCTAAGACTGGATGTGACGGCACTGCATCAGATAGTTGGAAAAAAGCATCAGAACCTGCTAGTAATTCGTCCTGGGGTGTTGCAGCAGCAACAGATAACAAGGATCAAGGTTCCGTCTGGGGCGGTAATGGTAGTCAAAAACCAAGTGTTGCAGATGATAGTGGTGACAAAGGTTCTGCTTGGAATAAGCCAGCTGAAGGTTCTTGGAGTAAACAAACTGGAGGATCCTCATGGGGTAAGCAAGCCGATTGGAGCACTAAAGAGGGATCAAGTACTGGTGATGCAGGGTGGAAAAGTTCAACGCCTTCAGTAGAGAATCAGACTGGGAGCTGGGGTAATGCAGGTGGCTGGGCAAAAGCTCAAGGCATATCTGGTAATGAGAATCATAAAGACAGTTGGAAGAAACCAAGTGATATAGATGACAATAAAAATGCTTCGTGGATCAAGGAAGATGGAGGTTCTACCTGGAATAAACAAGATGGAGGTTCTACTTGGAATAAGCCTGCTGGAGGTTCTTGGAGTAAAGGTGGGGATCAAGGGTCTGGTGGTGGCTATGGTGGCGATGGTGGTGGTGGTAGAGGGGGTGGAGGTGGGAACTGTTTTAAGTGCGGTGAGTCTGGACACATGTCTAGGGAGTGCCCTCAGGGCGGCGGTGGTGGTAGAGGGAGTGGAGGTGGGAACTGTTTCAAGTGCGGTGAGTCTGGACACATGTCTAGGGAGTGCCCTCAGGGTGGCAGTGGTGGTAGAGGGGGTGGAGGTGGGAATTGTTTCAAGTGCGGTGAGTCTGGACACATGTCTAGGGAGTGCCCTCAGGGTGGTGGTGGTGGTAGAGGGGGGAACTGTTACAAGTGTGGTGAATCTGGACACATGTCTAGGGAGTGCCCTCAGGGCGGAGGTGGTGGTAGAGGGGGTGGAGGTGGTAACTGTTTCAAGTGCGGTGAATCTGGACACATGTCTAGGGAGTGCCCTCAGGGCGGCGGTGGTGGTAGAGGGGCAGGAGGTGGTAACTGTTTCAAGTGCGGTGAGTCTGGACATATGTCAAGGGAGTGCCCTCAGGGCGGCGGTGGTGGTAGAGGGGCTGGAGGTGGGAACTGTTACAAGTGTGGTGAGTCTGGACACATGTCTAGGGAGTGCCCTCAGGGTGGCGGTGGTAGTAGAGGGAGTGGGGGTGGGAACTGTTTCAAATGTGGTGAGTCAGGACACATGTCTAGGGAGTGCCCTCAGGGCGGCGGTGGTAGAGGGGCTGGAAGTGGGAACTGTTTCAAGTGTGGTGAGTCTGGACACATGGCTAGGGACTGCTCTCAGGGAGGTGGTAGCTATGGCAACTTCGGTGGTGGAGCAAAGTCCACTGATTCATGGAGCAAGCGTGCTTGGGGTACTGGAGATGGTGGAAGTGGCGGTGATGGAGCAAAACCAGCAGCAAGCTCTTGGGGTACCACAGATGGTGGAAGTGGCTTTGGTGGTGGAGCAAAGCCCACTGATTCATGGAGCAAGCGTGTTTGGGGTACTGGAGATGGTGGAAGTGGCAGCGGTGGACCAAAACCAGCAGCAAGCTCTTGGGGTACAAATGGTGGAAGTGGCGGAGCTGGTGGTGAAGCAAAACCAGCAGGAAGCTCTTGGGGTAAGGGAGATGGTGGAAGTGGCAGAGCTAGTGATGAAGCAAAACCAGCAGCAAGCTCTTGGGGTACAGGAAGCTCTTGGGGTACAAATGGTGGAAGTGGCGGAGCTGGTGGTGAAGCAAAACCAGCAGGAAGCTCTTGGGGTAAGGGAGATGGAGGAAGTGGCGGAGCTAGTGGTGAAGCAAAACCAGCAGCAAGCTCTTGGGGTACAGGAAGCTCTTGGGGTAAGGCAGATGGTGGAAGTGGCAGAGCTAGTGGTGAAGCAAAACCAGCAGCAGCAAGCACTTGGGGTGCAGGAAGTTCCTGGGGTAAGGGAGATGGTGGAAGTGGCGGAGCTGGTGGTGAAGCAAAGCCTGCTGATCCATGGAGCAAGCCATCAGCAAGTGCTTGGGGTAAGGGAGATGGTGGAAGTGGCGGAGCTAGTGGTGAAGCAAAACCAGCAGCAAGCTCTTGGGGTACAGGAAGCTCTTGGGGTAAGGCAGATGGTGGAAGTGGCAGAGCTAGTGGTGAAGCAAAACCAGCTGCAAGCACTTGGGGTAAGGGAGATGGTGGAAGTGGCGGAGCTGGTGGTGAAGCAAAGCCTGCTGATCCATGGAGCAAGCCATCAGCAAGTGCTTGGGGTAAGAAAGATGGTGGAAGTGGCAGCGGAGGGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006351 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006368 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0006996 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016043 GO:0016070 GO:0016071 GO:0016246 GO:0016441 GO:0016458 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0030422 GO:0031047 GO:0031050 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032044 GO:0032259 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044728 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0071840 GO:0080090 GO:0080188 GO:0090304 GO:0097159 GO:0097659 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901698 GO:1901699 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Spt5-NGN

Protein Analysis

1672

Amino Acids

169.9

Weight (kDa)

6.79

Isoelectric Point (pI)

36.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Spt5-NGN PF03439 140 - 223 2e-19 Early transcription elongation factor of RNA pol II, NGN section
KOW1_SPT5 PF23042 233 - 356 9.6e-26 Transcription elongation factor SPT5, KOW1 domain
KOWx_SPT5 PF23037 437 - 487 2.6e-10 Transcription elongation factor SPT5, KOWx domain
KOW4_SPT5 PF23291 496 - 550 1.5e-21 Transcription elongation factor SPT5, fourth KOW domain
KOW5_SPT5 PF23290 610 - 654 2.2e-06 Transcription elongation factor SPT5, fifth KOW domain
zf-CCHC PF00098 1112 - 1128 2.2e-06 Zinc knuckle
zf-CCHC PF00098 1140 - 1156 2.2e-06 Zinc knuckle
zf-CCHC PF00098 1168 - 1184 2.2e-06 Zinc knuckle
zf-CCHC PF00098 1193 - 1209 1.1e-06 Zinc knuckle
zf-CCHC PF00098 1221 - 1237 2.2e-06 Zinc knuckle
zf-CCHC PF00098 1249 - 1265 2.2e-06 Zinc knuckle
zf-CCHC PF00098 1277 - 1293 1.1e-06 Zinc knuckle
zf-CCHC PF00098 1305 - 1321 2.2e-06 Zinc knuckle
zf-CCHC PF00098 1332 - 1348 2.9e-06 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1700, 3071
Acc36I ACCTGC 5 cut(s) 1700, 2407, 2545, 2803, 3071
Acc65I GGTACC 2 cut(s) 503, 4172
AccB1I GGYRCC 2 cut(s) 503, 4172
AccI GTMKAC 1 cut(s) 1581
AccII CGCG 1 cut(s) 447
AccIII TCCGGA 1 cut(s) 2005
AcoI YGGCCR 1 cut(s) 1887
AcsI RAATTY 5 cut(s) 290, 830, 1064, 1478, 1845
AcuI CTGAAG 9 cut(s) 369, 1230, 1251, 1281, 1523, 2067, 2156, 2955, 3030
AcyI GRCGYC 1 cut(s) 635
AfeI AGCGCT 1 cut(s) 1984
AflII CTTAAG 1 cut(s) 980
AflIII ACRYGT 7 cut(s) 3363, 3447, 3531, 3606, 3690, 3858, 3942
AjnI CCWGG 7 cut(s) 2276, 2411, 2549, 2687, 2810, 3203, 4661
AloI GAACNNNNNNTCC 2 cut(s) 1246, 1278
Alw21I GWGCWC 2 cut(s) 138, 2996
Alw26I GTCTC 2 cut(s) 445, 1610
AlwNI CAGNNNCTG 7 cut(s) 215, 1501, 2399, 2429, 2537, 2675, 2795
Ama87I CYCGRG 2 cut(s) 627, 2158
Aor13HI TCCGGA 1 cut(s) 2005
Aor51HI AGCGCT 1 cut(s) 1984
AoxI GGCC 4 cut(s) 3, 711, 1887, 2141
ApaI GGGCCC 1 cut(s) 715
ApoI RAATTY 5 cut(s) 290, 830, 1064, 1478, 1845
AsiSI GCGATCGC 1 cut(s) 30
Asp700I GAANNNNTTC 5 cut(s) 1793, 3422, 3506, 3917, 3998
Asp718I GGTACC 2 cut(s) 503, 4172
AspA2I CCTAGG 1 cut(s) 1690
AspLEI GCGC 5 cut(s) 1500, 1985, 2345, 2483, 2674
AspS9I GGNCC 6 cut(s) 711, 712, 1241, 1544, 1856, 4274
AsuC2I CCSGG 3 cut(s) 69, 114, 2092
AvaI CYCGRG 2 cut(s) 627, 2158
AvaII GGWCC 4 cut(s) 1241, 1544, 1856, 4274
AvrII CCTAGG 1 cut(s) 1690
AxyI CCTNAGG 8 cut(s) 3381, 3465, 3549, 3624, 3708, 3792, 3876, 3960
BaeGI GKGCMC 9 cut(s) 715, 3380, 3464, 3548, 3623, 3707, 3791, 3875, 3959
BaeI ACNNNNGTAYC 2 cut(s) 1861, 1894
BalI TGGCCA 1 cut(s) 1889
BamHI GGATCC 2 cut(s) 2002, 2963
BanI GGYRCC 2 cut(s) 503, 4172
BanII GRGCYC 1 cut(s) 715
BarI GAAGNNNNNNTAC 4 cut(s) 1041, 1073, 1565, 1597
Bbv12I GWGCWC 2 cut(s) 138, 2996
BceAI ACGGC 2 cut(s) 1881, 2773
BciT130I CCWGG 7 cut(s) 2278, 2413, 2551, 2689, 2812, 3205, 4663
BciVI GTATCC 4 cut(s) 934, 1862, 2368, 2644
BclI TGATCA 2 cut(s) 538, 882
BcnI CCSGG 3 cut(s) 69, 114, 2092
BcoDI GTCTC 2 cut(s) 445, 1610
BfmI CTRYAG 3 cut(s) 216, 512, 1582
BfoI RGCGCY 5 cut(s) 1501, 1986, 2346, 2484, 2675
BfrI CTTAAG 1 cut(s) 980
BfuAI ACCTGC 5 cut(s) 1700, 2407, 2545, 2803, 3071
BfuI GTATCC 4 cut(s) 934, 1862, 2368, 2644
BglI GCCNNNNNGGC 5 cut(s) 3384, 3627, 3711, 3795, 3963
BlnI CCTAGG 1 cut(s) 1690
BlpI GCTNAGC 1 cut(s) 1953
BmcAI AGTACT 1 cut(s) 3013
Bme18I GGWCC 4 cut(s) 1241, 1544, 1856, 4274
BmeT110I CYCGRG 2 cut(s) 627, 2158
BmgT120I GGNCC 6 cut(s) 711, 712, 1241, 1544, 1856, 4274
BmiI GGNNCC 8 cut(s) 505, 672, 713, 2004, 2259, 2851, 2965, 4174
BmrI ACTGGG 3 cut(s) 242, 1287, 3072
BmuI ACTGGG 3 cut(s) 242, 1287, 3072
BoxI GACNNNNGTC 9 cut(s) 2502, 3364, 3448, 3532, 3607, 3691, 3775, 3859, 3943
BplI GAGNNNNNCTC 6 cut(s) 440, 472, 2000, 2032, 2171, 2203
BpmI CTGGAG 9 cut(s) 107, 852, 1121, 2133, 2979, 3267, 3840, 4140, 4269
Bpu1102I GCTNAGC 1 cut(s) 1953
BpuEI CTTGAG 3 cut(s) 413, 920, 3084
BpuMI CCSGG 3 cut(s) 69, 114, 2092
Bsa29I ATCGAT 1 cut(s) 279
BsaHI GRCGYC 1 cut(s) 635
BsaWI WCCGGW 2 cut(s) 944, 2005
Bse21I CCTNAGG 8 cut(s) 3381, 3465, 3549, 3624, 3708, 3792, 3876, 3960
Bse3DI GCAATG 1 cut(s) 825
BseAI TCCGGA 1 cut(s) 2005
BseBI CCWGG 7 cut(s) 2278, 2413, 2551, 2689, 2812, 3205, 4663
BseCI ATCGAT 1 cut(s) 279
BseGI GGATG 8 cut(s) 991, 1705, 1711, 2273, 2408, 2546, 2684, 2756
BseMI GCAATG 1 cut(s) 825
BseRI GAGGAG 5 cut(s) 284, 648, 663, 917, 1067
BseSI GKGCMC 9 cut(s) 715, 3380, 3464, 3548, 3623, 3707, 3791, 3875, 3959
BseYI CCCAGC 6 cut(s) 2078, 2319, 2457, 2733, 3069, 3087
BsgI GTGCAG 1 cut(s) 4671
Bsh1236I CGCG 1 cut(s) 447
Bsh1285I CGRYCG 1 cut(s) 30
BshFI GGCC 4 cut(s) 5, 713, 1889, 2143
BshNI GGYRCC 2 cut(s) 503, 4172
BshVI ATCGAT 1 cut(s) 279
BsiEI CGRYCG 1 cut(s) 30
BsiHKAI GWGCWC 2 cut(s) 138, 2996
BsiHKCI CYCGRG 2 cut(s) 627, 2158
BsiSI CCGG 6 cut(s) 68, 84, 114, 945, 2006, 2091
BslFI GGGAC 2 cut(s) 2095, 4046
BsmAI GTCTC 2 cut(s) 445, 1610
BsmFI GGGAC 2 cut(s) 2095, 4046
BsmI GAATGC 2 cut(s) 2113, 2140
BsnI GGCC 4 cut(s) 5, 713, 1889, 2143
BsoBI CYCGRG 2 cut(s) 627, 2158
Bsp120I GGGCCC 1 cut(s) 711
Bsp13I TCCGGA 1 cut(s) 2005
Bsp1720I GCTNAGC 1 cut(s) 1953
Bsp19I CCATGG 3 cut(s) 1772, 4721, 4949
BspANI GGCC 4 cut(s) 5, 713, 1889, 2143
BspDI ATCGAT 1 cut(s) 279
BspEI TCCGGA 1 cut(s) 2005
BspFNI CGCG 1 cut(s) 447
BspLI GGNNCC 8 cut(s) 505, 672, 713, 2004, 2259, 2851, 2965, 4174
BspMAI CTGCAG 1 cut(s) 516
BspMI ACCTGC 5 cut(s) 1700, 2407, 2545, 2803, 3071
BspQI GCTCTTC 1 cut(s) 1136
BspT107I GGYRCC 2 cut(s) 503, 4172
BspTI CTTAAG 1 cut(s) 980
BsrDI GCAATG 1 cut(s) 825
BssNI GRCGYC 1 cut(s) 635
BssT1I CCWWGG 4 cut(s) 1690, 1772, 4721, 4949
Bst2UI CCWGG 7 cut(s) 2278, 2413, 2551, 2689, 2812, 3205, 4663
Bst6I CTCTTC 3 cut(s) 716, 1044, 1136
BstACI GRCGYC 1 cut(s) 635
BstAFI CTTAAG 1 cut(s) 980
BstAPI GCANNNNNTGC 3 cut(s) 824, 4712, 4940
BstDSI CCRYGG 3 cut(s) 1772, 4721, 4949
BstENI CCTNNNNNAGG 2 cut(s) 1694, 3246
BstF5I GGATG 8 cut(s) 991, 1705, 1711, 2273, 2408, 2546, 2684, 2756
BstFNI CGCG 1 cut(s) 447
BstH2I RGCGCY 5 cut(s) 1501, 1986, 2346, 2484, 2675
BstHHI GCGC 5 cut(s) 1500, 1985, 2345, 2483, 2674
BstMAI GTCTC 2 cut(s) 445, 1610
BstMCI CGRYCG 1 cut(s) 30
BstNI CCWGG 7 cut(s) 2278, 2413, 2551, 2689, 2812, 3205, 4663
BstNSI RCATGY 8 cut(s) 600, 3367, 3451, 3535, 3610, 3694, 3862, 3946
BstPAI GACNNNNGTC 9 cut(s) 2502, 3364, 3448, 3532, 3607, 3691, 3775, 3859, 3943
BstSFI CTRYAG 3 cut(s) 216, 512, 1582
BstSLI GKGCMC 9 cut(s) 715, 3380, 3464, 3548, 3623, 3707, 3791, 3875, 3959
BstUI CGCG 1 cut(s) 447
BstX2I RGATCY 4 cut(s) 409, 1421, 2002, 2963
BstYI RGATCY 4 cut(s) 409, 1421, 2002, 2963
Bsu15I ATCGAT 1 cut(s) 279
Bsu36I CCTNAGG 8 cut(s) 3381, 3465, 3549, 3624, 3708, 3792, 3876, 3960
BsuI GTATCC 4 cut(s) 934, 1862, 2368, 2644
BsuRI GGCC 4 cut(s) 5, 713, 1889, 2143
BsuTUI ATCGAT 1 cut(s) 279
BtgI CCRYGG 3 cut(s) 1772, 4721, 4949
BtgZI GCGATG 2 cut(s) 87, 3315
BtsCI GGATG 8 cut(s) 991, 1705, 1711, 2273, 2408, 2546, 2684, 2756
BtsI GCAGTG 3 cut(s) 1159, 2760, 3481
BtsIMutI CAGTG 6 cut(s) 694, 1159, 2760, 3481, 4086, 4215
BveI ACCTGC 5 cut(s) 1700, 2407, 2545, 2803, 3071
CaiI CAGNNNCTG 7 cut(s) 215, 1501, 2399, 2429, 2537, 2675, 2795
CfoI GCGC 5 cut(s) 1500, 1985, 2345, 2483, 2674
Cfr13I GGNCC 6 cut(s) 711, 712, 1241, 1544, 1856, 4274
ClaI ATCGAT 1 cut(s) 279
CseI GACGC 3 cut(s) 453, 643, 1504
CspCI CAANNNNNGTGG 2 cut(s) 4051, 4086
EaeI YGGCCR 1 cut(s) 1887
Eam1104I CTCTTC 3 cut(s) 716, 1044, 1136
EarI CTCTTC 3 cut(s) 716, 1044, 1136
Eco130I CCWWGG 4 cut(s) 1690, 1772, 4721, 4949
Eco24I GRGCYC 1 cut(s) 715
Eco32I GATATC 2 cut(s) 277, 1906
Eco47I GGWCC 4 cut(s) 1241, 1544, 1856, 4274
Eco47III AGCGCT 1 cut(s) 1984
Eco57I CTGAAG 9 cut(s) 369, 1230, 1251, 1281, 1523, 2067, 2156, 2955, 3030
Eco81I CCTNAGG 8 cut(s) 3381, 3465, 3549, 3624, 3708, 3792, 3876, 3960
Eco88I CYCGRG 2 cut(s) 627, 2158
EcoNI CCTNNNNNAGG 2 cut(s) 1694, 3246
EcoO109I RGGNCCY 1 cut(s) 1241
EcoRI GAATTC 1 cut(s) 1845
EcoRII CCWGG 7 cut(s) 2276, 2411, 2549, 2687, 2810, 3203, 4661
EcoRV GATATC 2 cut(s) 277, 1906
EcoT14I CCWWGG 4 cut(s) 1690, 1772, 4721, 4949
EcoT38I GRGCYC 1 cut(s) 715
ErhI CCWWGG 4 cut(s) 1690, 1772, 4721, 4949
FalI AAGNNNNNCTT 8 cut(s) 186, 218, 2320, 2352, 2458, 2490, 2596, 2628
FaqI GGGAC 2 cut(s) 2095, 4046
FauI CCCGC 1 cut(s) 1022
FauNDI CATATG 2 cut(s) 334, 3774
FbaI TGATCA 2 cut(s) 538, 882
FblI GTMKAC 1 cut(s) 1581
FokI GGATG 8 cut(s) 998, 1712, 1718, 2260, 2395, 2533, 2671, 2763
FriOI GRGCYC 1 cut(s) 715
GlaI GCGC 5 cut(s) 1499, 1984, 2344, 2482, 2673
GsaI CCCAGC 6 cut(s) 2082, 2323, 2461, 2737, 3073, 3091
GsuI CTGGAG 9 cut(s) 107, 852, 1121, 2133, 2979, 3267, 3840, 4140, 4269
HaeII RGCGCY 5 cut(s) 1501, 1986, 2346, 2484, 2675
HaeIII GGCC 4 cut(s) 5, 713, 1889, 2143
HapII CCGG 6 cut(s) 68, 84, 114, 945, 2006, 2091
HgaI GACGC 3 cut(s) 453, 643, 1504
HhaI GCGC 5 cut(s) 1500, 1985, 2345, 2483, 2674
Hin1I GRCGYC 1 cut(s) 635
Hin6I GCGC 5 cut(s) 1498, 1983, 2343, 2481, 2672
HinP1I GCGC 5 cut(s) 1498, 1983, 2343, 2481, 2672
HincII GTYRAC 1 cut(s) 487
HindII GTYRAC 1 cut(s) 487
HindIII AAGCTT 2 cut(s) 1670, 1916
HpaII CCGG 6 cut(s) 68, 84, 114, 945, 2006, 2091
Hpy166II GTNNAC 8 cut(s) 230, 487, 766, 1275, 1582, 2951, 4086, 4274
Hpy8I GTNNAC 8 cut(s) 230, 487, 766, 1275, 1582, 2951, 4086, 4274
Hpy99I CGWCG 2 cut(s) 170, 2374
HpyCH4IV ACGT 3 cut(s) 585, 1683, 2253
HpySE526I ACGT 3 cut(s) 585, 1683, 2253
Hsp92I GRCGYC 1 cut(s) 635
HspAI GCGC 5 cut(s) 1498, 1983, 2343, 2481, 2672
Kpn2I TCCGGA 1 cut(s) 2005
KpnI GGTACC 2 cut(s) 507, 4176
Ksp22I TGATCA 2 cut(s) 538, 882
LguI GCTCTTC 1 cut(s) 1136
MaeII ACGT 3 cut(s) 585, 1683, 2253
MflI RGATCY 4 cut(s) 409, 1421, 2002, 2963
MlsI TGGCCA 1 cut(s) 1889
MluNI TGGCCA 1 cut(s) 1889
MmeI TCCRAC 8 cut(s) 77, 421, 2332, 2352, 2470, 2608, 2756, 3116
Mox20I TGGCCA 1 cut(s) 1889
MroI TCCGGA 1 cut(s) 2005
MroXI GAANNNNTTC 5 cut(s) 1793, 3422, 3506, 3917, 3998
MscI TGGCCA 1 cut(s) 1889
MseI TTAA 9 cut(s) 981, 1022, 1530, 1841, 1862, 1914, 1947, 2022, 3342
Msp20I TGGCCA 1 cut(s) 1889
MspA1I CMGCKG 5 cut(s) 2429, 2933, 4269, 4880, 5004
MspCI CTTAAG 1 cut(s) 980
MspI CCGG 6 cut(s) 68, 84, 114, 945, 2006, 2091
Mva1269I GAATGC 2 cut(s) 2113, 2140
MvaI CCWGG 7 cut(s) 2278, 2413, 2551, 2689, 2812, 3205, 4663
MvnI CGCG 1 cut(s) 447
NciI CCSGG 3 cut(s) 69, 114, 2092
NcoI CCATGG 3 cut(s) 1772, 4721, 4949
NdeI CATATG 2 cut(s) 334, 3774
NlaIV GGNNCC 8 cut(s) 505, 672, 713, 2004, 2259, 2851, 2965, 4174
NmuCI GTSAC 9 cut(s) 581, 631, 740, 1124, 1432, 2495, 2633, 2753, 2903
NspI RCATGY 8 cut(s) 600, 3367, 3451, 3535, 3610, 3694, 3862, 3946
PaqCI CACCTGC 2 cut(s) 1700, 3071
PciI ACATGT 7 cut(s) 3363, 3447, 3531, 3606, 3690, 3858, 3942
PciSI GCTCTTC 1 cut(s) 1136
PcsI WCGNNNNNNNCGW 2 cut(s) 162, 171
PctI GAATGC 2 cut(s) 2113, 2140
PdmI GAANNNNTTC 5 cut(s) 1793, 3422, 3506, 3917, 3998
Ple19I CGATCG 1 cut(s) 30
PpuMI RGGWCCY 1 cut(s) 1241
PscI ACATGT 7 cut(s) 3363, 3447, 3531, 3606, 3690, 3858, 3942
PshAI GACNNNNGTC 9 cut(s) 2502, 3364, 3448, 3532, 3607, 3691, 3775, 3859, 3943
Psp5II RGGWCCY 1 cut(s) 1241
Psp6I CCWGG 7 cut(s) 2276, 2411, 2549, 2687, 2810, 3203, 4661
PspFI CCCAGC 6 cut(s) 2078, 2319, 2457, 2733, 3069, 3087
PspGI CCWGG 7 cut(s) 2276, 2411, 2549, 2687, 2810, 3203, 4661
PspN4I GGNNCC 8 cut(s) 505, 672, 713, 2004, 2259, 2851, 2965, 4174
PspOMI GGGCCC 1 cut(s) 711
PspPI GGNCC 6 cut(s) 711, 712, 1241, 1544, 1856, 4274
PspPPI RGGWCCY 1 cut(s) 1241
PstI CTGCAG 1 cut(s) 516
PstNI CAGNNNCTG 7 cut(s) 215, 1501, 2399, 2429, 2537, 2675, 2795
PsuI RGATCY 4 cut(s) 409, 1421, 2002, 2963
PvuI CGATCG 1 cut(s) 30
PvuII CAGCTG 3 cut(s) 2429, 2933, 4880
RgaI GCGATCGC 1 cut(s) 30
SapI GCTCTTC 1 cut(s) 1136
SaqAI TTAA 9 cut(s) 981, 1022, 1530, 1841, 1862, 1914, 1947, 2022, 3342
Sau96I GGNCC 6 cut(s) 711, 712, 1241, 1544, 1856, 4274
ScaI AGTACT 1 cut(s) 3013
SfaAI GCGATCGC 1 cut(s) 30
SfcI CTRYAG 3 cut(s) 216, 512, 1582
SgfI GCGATCGC 1 cut(s) 30
SinI GGWCC 4 cut(s) 1241, 1544, 1856, 4274
SmlI CTYRAG 4 cut(s) 392, 899, 980, 3099
SmoI CTYRAG 4 cut(s) 392, 899, 980, 3099
SspI AATATT 1 cut(s) 1596
StyI CCWWGG 4 cut(s) 1690, 1772, 4721, 4949
TaiI ACGT 3 cut(s) 588, 1686, 2256
TaqI TCGA 3 cut(s) 132, 279, 2057
TaqII GACCGA 1 cut(s) 1820
TatI WGTACW 2 cut(s) 1462, 3011
TauI GCSGC 7 cut(s) 53, 146, 2702, 3390, 3717, 3801, 3969
Tru1I TTAA 9 cut(s) 981, 1022, 1530, 1841, 1862, 1914, 1947, 2022, 3342
Tru9I TTAA 9 cut(s) 981, 1022, 1530, 1841, 1862, 1914, 1947, 2022, 3342
TscAI CASTG 6 cut(s) 694, 1159, 2767, 3481, 4093, 4222
TseFI GTSAC 9 cut(s) 581, 631, 740, 1124, 1432, 2495, 2633, 2753, 2903
Tsp45I GTSAC 9 cut(s) 581, 631, 740, 1124, 1432, 2495, 2633, 2753, 2903
TspGWI ACGGA 2 cut(s) 1830, 2842
TspRI CASTG 6 cut(s) 694, 1159, 2767, 3481, 4093, 4222
Vha464I CTTAAG 1 cut(s) 980
VpaK11BI GGWCC 4 cut(s) 1241, 1544, 1856, 4274
XagI CCTNNNNNAGG 2 cut(s) 1694, 3246
XapI RAATTY 5 cut(s) 290, 830, 1064, 1478, 1845
XceI RCATGY 8 cut(s) 600, 3367, 3451, 3535, 3610, 3694, 3862, 3946
XmaJI CCTAGG 1 cut(s) 1690
XmiI GTMKAC 1 cut(s) 1581
XmnI GAANNNNTTC 5 cut(s) 1793, 3422, 3506, 3917, 3998
ZrmI AGTACT 1 cut(s) 3013
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.