MD07G1167800.v1.1

Transcription elongation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0012966
Physical Location & Seq
Reverse (-)
24234858 .. 24242333
7476 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1167800.v1.1.491

Sequence Viewer

Length: 4587 bp
ATGGCGTCGAAGGGAAAGGGGATCGCCGCCGGAGGTGGATTCTCCGGCGGGAAGCGCAAGCACGACGACGACAAGTCCGGTGGTGGCCGGAAGAGGAGCAACCCCGGCGTCCTCAGATTCTTCGAAGACGCCGCTGCCGAATCCGACGACGAAATCGACAGTGATTTCGATGACGATTTTATGGACGAAGAATTTGAAACAGAACCTGTAGCCCAGAATGACCCAGGGAAAGCACATAACCTTCCATTCGTTCCTAAAGAGGAGGATCTGGATGGTGAAGAATTTGAAAAAATGATGGAAGAACGATACAGGAGTGGTTCTAGCTATGCAACCTATGCTGAAGATAATTTTGAGAACAAAAGGTCGATTGATGGAAATGTTCTGCCTTCTGTTAAGGATCCAGTCATCTGGAAAGTGAAATGCATGGTTGGACGTGAGAGGCATTCAGCTTTCTGTATGATGCAAAAGTTTGTTGACTTCCGATCACTAGGTACCAAACTACAGATTGTTTCTGCATTTGCTGTTGAGCACATCAAGGGTTTTGTTTTCATTGAAGCTGACAAGCAGTCTGATATTAATGAGGCATGTAAAGGAATTTGTAGTATATACCCTTCCCGAGTAATGCCAGTTCCAAATAACGAAGTTTCTCATTTGCTGTCTCCTCGGACTAAATCCAATGGAATTACTGCTGGCATGTGGGCCCGTGTGAAGAATGGGAACTACAAGGGTGATTTGGCACAGGTTGTGTTTGTGAACGATTTACGAAAAAGAGCGACCTTAAAGCTGATCCCCAGAATTGATCTTCAAGCAATGGCTGCAAAATTTGGTGGAGGAGGTACTAGAAAGAAAAGGCCTGCTCCTGCCCCGAGATTGATCAGCTCAAACGAACTCGAGGAGTTCCGCCCTCTCATTCAGTACAGGACTGATCGTGAAAGTGGCATGAAGTTTGAGTTTCTTGATGGTCTGATGTTCAAGGATGGATATTTGTACAAAAAAGTATCAATAGATTCATTAAGCTTCTGGGGTGTTATGCCATCAGAAGAGGAACTACTGAAATTCAAGCCTTCTGAGAACACCAAAAATGAGGATGAGGACTGGCTTACTGAACTTTATGGTACAACAGAAAAGAAAAGGCGGACCATCAAAATTGAAGAAGGTGGTGGTAAAGGAGAGGGTTCATCAGGTTCTATGGGGAAAGGAGAGGGTTCATCAGGGTCTATGGGGAAAGGAGAGGGCTCATCAGGGTCTATGGGGAAAGGAGAGGGTTCATCAGGACCTATGGGGAAAGGAGAGGGCTCATCGCAGTCTATGGGGAAAGGAGAGGGTTCATCTGGGTCCAAGGGGAAAGGAGAAGGTTCATCAGGTTCTATGGGGAAGGGAGAGGACTCATCAACGTCTATGGGGAAAGGCGGGGGTTCTTCAGGTTCTGGTGGGAGTGGTTTTGAACTGTATGATCTTGTTTGTTTTGGCCGGAAAGATTTTGGTCTTGTAATAGGCATGGAGAAAGATGATAGTTACAAGATTCTGAAAGAGGGAGTGGAAGGACCTATTGTGCTGATGGTTCAGAAACGTGAGTTAAAGAATGTTCTTTCTGATATGAAATTTACGGCTTTAGATCGGCACATGAAGGCCATATGCGTTAGTGATACTGTCAAAGTGTCGGAAGGACCGTTGGAGGGTAGGCAGGGTATTGTTAAGCAAATATATAGAGGCACCATATTCTTGTATGATGAGAACCAGTCAGAAAATGGTGGTTATTTTTGCTCAAAATCCCAAATGTGTGAGAAAGTTAAGCTTTACATTGATTCTAGCAAAGAAATGGATGGGGACTCAGGTGCTCTGGGTTTTGGAGATTTCATGTCGTCTCCTAAATCCCCGCTATCGCCAAAAAAGCCATGGCAAGAAAGGGATAGTAACTTCAATCAGGGTGATAAAGATGGAATGTTCTCAATTGGTCAAACTGTAAGAATCCGTGTGGGTCCGTTGAAGGGATACCTCTGTCGAATCTTGGCTATACGTCGTGCTGATATTACTGTTAAGCTTGATTCTCAGCAAAAGGTTCTCACAGTTACAAAAGAGCATATTTCTGAAGTTCGTGGAAAGAGTTCTAGTGTGCTGATTAGTGAGGATCCGGAGTCCAGTTTAAAACCATTTGATTTGCTCGGAACGGAAGGAGGGTCTAATGACTGGACAAATGGAGCTGGGGTATCTGCTGGGGGTGATGGGTGGAATGCTGGAGGGGCATCAGCCGGGGGTGGTGGGTGGAATGCTGGAGGGGCATCTGGTGATAGTAATTCTTGGGCTAGTGCAAATCCTATTAATGATGTGAAAAAGGATGATGATACTTCTTGGGGGAGCAAAGCAGCTCCAAGTACAGCTTCGTCCTGGGGTGCAGCAGCAGGAGCACCTGCAGATAACAACAATCAAGGTGCTGGCTGGGGAAAAAGTGATGCTTGGGGAAAATCAAGTGCCAAAACTGAAGGTGACAGTAGTGCGCCAGATAACAATGATCAAGGTGCTGGCTGGGGAAAAAGAGACACTTGGGGAAAATCAAGTGCTAAAACTGGGGCTGACAGCAGCGCACCAGCAGATAACAATGATCAAGGCACTGGCTGGGGAAAAAGCGACTCATGGGGGAAATCAAGTTCTAAAACTGGGGGTGACAGCAGTGCCCCACATAACAATGATCAGGGTGCTGGCTGGGGAAGAAGTGGCTCTTGGGGGCAGAAGGCCGAACCTGCTGGGACGGGTCAACTGGATTCATGGGGCAAGGGTAAAAATGTTGTTGAAGCAGGATCATGGGGAAAGAATTCTGATGCACCATCTGGGGATATTTCAAGTCCTGGGTGGGGTCAACAGAAATCTTGGGACAAAGGAAATGCAGTCAGTAGTGATGGGCCTGCTTGGGGTAAGCCTCAGAATAAAGCCACAGGGAACTGGAGCAGTAAAGATGAATCAAGTGCTGGCGATGCAGGGTGGAAAAGTTCAGTACCAGCTGAAAATGTTCAGACTGGAAGCTGGGGTAAGGCAGGTGGCGGTTCAACCGAGAGTAAACAAGATGGCAGTTCTTCTTGGGGTAAGCCAGCTGGAGATTCATGGGGTAAACAAACTGGAGGCTCTTCATGGGGTAAACAAGCTGATGTAACCGCTGGCGGAGTGGGGAACAACGGAGGCAAGTGGGGCAAAGGATCTGTCACCAATGAAGAACAAAGTGGTGGAGGGGGAGATCAGGTTTCTAAATGGGGCCAAAAAGGCACTTGGAACTCAGGATCTAGTGAGACTGGTGGCAATCAAGAATTTAGTTGGGGTAAGAAAAGCGATGGGAACATCGGATCTACTTCCTCAGGTGGGAACCAAAATTCTACTTGGGGCAAGACAATCAATTTGAACTCTGGATCTGGTGACACTGGTGGAAATCTTGATTCTAGTTGGGGGAAGAAGAGTACTTGGAATTCTCAATCTAGTGAGAATAGTGGCAATCAAGATTCTAGTTGGGGGAAGAAAAGTGATTGGAAATCTGGATCTGATGACACCGGTGGGAACCATGATTCAACTTGGGGCAAAAAAAGTAGTTGGAACTCAGGATCTGGTGATGCAGATCAGAAATCTAGTTGGGGCAGTAAAAGCAGTTGGAATTCAACAGATGGTGGGAATGGAGACCAACCTGAGGATTCAAGTGGTGGGAGAGGTGGACGGGGTTTTGGAGATGGTGGGCGTAGAGGGGGTTTTGGGGGAAGAGGTGGAGGCGGGGGCAGTGGTGCTTGCTTCAAATGTGGTGAGTCCGGGCACATGTCGAGGGAGTGTCCCCAGGGCGGTGGAGGTGGTAAAGGTGGTGCTTGCTTCAAGTGTGGTGAGTCTGGGCACATGTCGAGGGAGTGCCCCCAGGGCGGTGGAGGCGGTAAAGGTGGTGCTTGCTTCAAGTGTGGTGAGTCCGGGCACATGTCGAGGGAGTGCCCCCAGGGTGGTGGAGGCAGTAAAGGTGGTGCTTGCTTCAAGTGTGGTGAGTCCGGGCACATGTCAAGGGAGTGCCCCCAGGGTGGTGGAGGCGGTAAAGGTGGTGCTTGCTTCAAATGTGGTGAGGCAGGGCACATGTCGAGGGAGTGCCCCCAGGGCGGTGGAGGTGGTGGAGGTGGTGCTTGCTTCAAGTGCGGTGAGTCCGGGCACATGGCAAGGGACTGCTCCCAAGGCGGTGGGGGCTATGGTGGTGGCAACCGCAGCGGTGGTGGAGGTGGTGGTGCTTGCTACAAGTGTGGTGAGTCGGGGCACATGGCGAGGGACTGCTCCCAGGGCGGTGGAAGCTATGGTGGTGGCAGCCGTAGCGGTGGAGGTGGCAGCAGTGGTGCTTGCTTCAAGTGTGGTGAGTCAGGGCACATGGCGAGGGAGTGCCCCCAGGGCGGTGGAGGTGGTAGCAGTGGTGCTTGCTACAAATGTGGTGAGTCTGGGCACATGGCGAGGGATTGCTCCCAGGGCGGTGGAGGCTATGGTGGTGGTGGCCGTAGCGGTGGTGGAGGCGGTGGTGCTTCCGGTGGCTGCTACAAGTGCGGACAGTCCGGCCATTTCGCAAGGGAGTGCCCCAACAGTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006351 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006368 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0006996 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016043 GO:0016070 GO:0016071 GO:0016246 GO:0016441 GO:0016458 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0030422 GO:0031047 GO:0031050 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032044 GO:0032259 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044728 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0071840 GO:0080090 GO:0080188 GO:0090304 GO:0097159 GO:0097659 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901698 GO:1901699 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Spt5-NGN

Protein Analysis

1529

Amino Acids

157.24

Weight (kDa)

6.72

Isoelectric Point (pI)

46.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Spt5-NGN PF03439 136 - 219 2e-19 Early transcription elongation factor of RNA pol II, NGN section
KOW1_SPT5 PF23042 231 - 353 2.5e-28 Transcription elongation factor SPT5, KOW1 domain
KOWx_SPT5 PF23037 479 - 528 2.1e-09 Transcription elongation factor SPT5, KOWx domain
KOW4_SPT5 PF23291 537 - 591 1.7e-23 Transcription elongation factor SPT5, fourth KOW domain
KOW5_SPT5 PF23290 651 - 695 1e-07 Transcription elongation factor SPT5, fifth KOW domain
zf-CCHC PF00098 1254 - 1270 8.2e-07 Zinc knuckle
zf-CCHC PF00098 1279 - 1295 8.2e-07 Zinc knuckle
zf-CCHC PF00098 1304 - 1320 8.2e-07 Zinc knuckle
zf-CCHC PF00098 1329 - 1345 8.2e-07 Zinc knuckle
zf-CCHC PF00098 1354 - 1370 1.1e-06 Zinc knuckle
zf-CCHC PF00098 1379 - 1395 1.1e-06 Zinc knuckle
zf-CCHC PF00098 1413 - 1429 5.4e-07 Zinc knuckle
zf-CCHC PF00098 1448 - 1464 4.3e-07 Zinc knuckle
zf-CCHC PF00098 1473 - 1489 5.4e-07 Zinc knuckle
zf-CCHC PF00098 1510 - 1526 8.5e-07 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 2416, 3020
Acc36I ACCTGC 3 cut(s) 2416, 2746, 3020
Acc65I GGTACC 1 cut(s) 491
AccB1I GGYRCC 2 cut(s) 491, 1712
AccIII TCCGGA 1 cut(s) 2131
AcoI YGGCCR 4 cut(s) 85, 1468, 4492, 4552
AcuI CTGAAG 4 cut(s) 360, 1404, 2109, 2499
AcyI GRCGYC 3 cut(s) 5, 108, 129
AfaI GTAC 8 cut(s) 493, 838, 917, 989, 1117, 2374, 2991, 3445
AflIII ACRYGT 5 cut(s) 3789, 3864, 3939, 4014, 4089
AgeI ACCGGT 1 cut(s) 3533
AhdI GACNNNNNGTC 2 cut(s) 73, 565
AjiI CACGTC 1 cut(s) 434
AjuI GAANNNNNNNTTGG 4 cut(s) 2362, 2394, 2701, 2733
AloI GAACNNNNNNTCC 2 cut(s) 2629, 2661
Alw21I GWGCWC 3 cut(s) 531, 1840, 2407
Alw26I GTCTC 5 cut(s) 663, 1869, 2529, 3272, 3651
AlwNI CAGNNNCTG 3 cut(s) 206, 1427, 3587
Ama87I CYCGRG 3 cut(s) 615, 865, 890
Aor13HI TCCGGA 1 cut(s) 2131
ApaI GGGCCC 1 cut(s) 703
ArsI GACNNNNNNTTYG 8 cut(s) 149, 176, 181, 208, 3177, 3209, 3367, 3399
AseI ATTAAT 2 cut(s) 576, 2319
AsiGI ACCGGT 1 cut(s) 3533
Asp700I GAANNNNTTC 3 cut(s) 2104, 2809, 3003
Asp718I GGTACC 1 cut(s) 491
AspLEI GCGC 3 cut(s) 57, 2497, 2582
AsuC2I CCSGG 6 cut(s) 105, 2251, 3784, 3934, 4009, 4159
AsuII TTCGAA 1 cut(s) 123
AvaI CYCGRG 3 cut(s) 615, 865, 890
AvaII GGWCC 6 cut(s) 1137, 1274, 1335, 1544, 1667, 1979
AxyI CCTNAGG 2 cut(s) 3343, 3666
BaeI ACNNNNGTAYC 4 cut(s) 298, 331, 1984, 2017
BamHI GGATCC 2 cut(s) 397, 2128
BanI GGYRCC 2 cut(s) 491, 1712
BanII GRGCYC 3 cut(s) 703, 1238, 1298
BarI GAAGNNNNNNTAC 4 cut(s) 595, 627, 1032, 1064
BbsI GAAGAC 1 cut(s) 132
Bbv12I GWGCWC 3 cut(s) 531, 1840, 2407
BceAI ACGGC 3 cut(s) 1623, 4299, 4479
BciVI GTATCC 1 cut(s) 1985
BclI TGATCA 4 cut(s) 873, 2509, 2599, 2686
BcnI CCSGG 6 cut(s) 105, 2251, 3784, 3934, 4009, 4159
BcoDI GTCTC 5 cut(s) 663, 1869, 2529, 3272, 3651
BfmI CTRYAG 3 cut(s) 207, 500, 2409
BfuAI ACCTGC 3 cut(s) 2416, 2746, 3020
BfuI GTATCC 1 cut(s) 1985
BglI GCCNNNNNGGC 4 cut(s) 3252, 3885, 4110, 4392
BmcAI AGTACT 1 cut(s) 3445
Bme18I GGWCC 6 cut(s) 1137, 1274, 1335, 1544, 1667, 1979
BmeRI GACNNNNNGTC 2 cut(s) 73, 565
BmeT110I CYCGRG 3 cut(s) 615, 865, 890
BmgBI CACGTC 1 cut(s) 434
BmrI ACTGGG 2 cut(s) 2574, 2664
BmsI GCATC 7 cut(s) 450, 2252, 2288, 2440, 2806, 2959, 3583
BmuI ACTGGG 2 cut(s) 2574, 2664
BpiI GAAGAC 1 cut(s) 132
BpmI CTGGAG 5 cut(s) 2256, 2292, 2959, 3108, 3132
Bpu14I TTCGAA 1 cut(s) 123
BpuMI CCSGG 6 cut(s) 105, 2251, 3784, 3934, 4009, 4159
BsaBI GATNNNNATC 1 cut(s) 3597
BsaHI GRCGYC 3 cut(s) 5, 108, 129
BsaI GGTCTC 1 cut(s) 3651
BsaWI WCCGGW 4 cut(s) 77, 2131, 3533, 4523
BsaXI ACNNNNNCTCC 4 cut(s) 2190, 2220, 3216, 3246
Bse118I RCCGGY 1 cut(s) 3533
Bse21I CCTNAGG 2 cut(s) 3343, 3666
Bse3DI GCAATG 1 cut(s) 816
Bse8I GATNNNNATC 1 cut(s) 3597
BseAI TCCGGA 1 cut(s) 2131
BseGI GGATG 5 cut(s) 277, 982, 1093, 1828, 2341
BseJI GATNNNNATC 1 cut(s) 3597
BseMI GCAATG 1 cut(s) 816
BseMII CTCAG 9 cut(s) 127, 1059, 1845, 2063, 2930, 3279, 3357, 3594, 3657
BseRI GAGGAG 5 cut(s) 109, 275, 651, 846, 908
BseYI CCCAGC 8 cut(s) 2201, 2213, 2436, 2523, 2613, 2700, 2741, 3018
BsgI GTGCAG 1 cut(s) 2412
BshNI GGYRCC 2 cut(s) 491, 1712
BshTI ACCGGT 1 cut(s) 3533
BsiHKAI GWGCWC 3 cut(s) 531, 1840, 2407
BsiHKCI CYCGRG 3 cut(s) 615, 865, 890
BslFI GGGAC 6 cut(s) 1841, 2758, 2882, 3789, 4187, 4289
BsmAI GTCTC 5 cut(s) 663, 1869, 2529, 3272, 3651
BsmBI CGTCTC 1 cut(s) 1869
BsmFI GGGAC 6 cut(s) 1841, 2758, 2882, 3789, 4187, 4289
BsmI GAATGC 3 cut(s) 442, 2236, 2272
Bso31I GGTCTC 1 cut(s) 3651
BsoBI CYCGRG 3 cut(s) 615, 865, 890
Bsp119I TTCGAA 1 cut(s) 123
Bsp120I GGGCCC 1 cut(s) 699
Bsp13I TCCGGA 1 cut(s) 2131
Bsp1407I TGTACA 1 cut(s) 987
Bsp19I CCATGG 1 cut(s) 1895
BspCNI CTCAG 9 cut(s) 126, 1060, 1844, 2062, 2929, 3278, 3356, 3593, 3658
BspEI TCCGGA 1 cut(s) 2131
BspMAI CTGCAG 1 cut(s) 2413
BspMI ACCTGC 3 cut(s) 2416, 2746, 3020
BspQI GCTCTTC 1 cut(s) 3124
BspT104I TTCGAA 1 cut(s) 123
BspT107I GGYRCC 2 cut(s) 491, 1712
BspTNI GGTCTC 1 cut(s) 3651
BsrDI GCAATG 1 cut(s) 816
BsrFI RCCGGY 1 cut(s) 3533
BsrGI TGTACA 1 cut(s) 987
BssAI RCCGGY 1 cut(s) 3533
BssNI GRCGYC 3 cut(s) 5, 108, 129
BssT1I CCWWGG 3 cut(s) 1338, 1895, 4183
Bst6I CTCTTC 5 cut(s) 86, 1035, 3124, 3434, 3730
BstACI GRCGYC 3 cut(s) 5, 108, 129
BstAPI GCANNNNNTGC 2 cut(s) 335, 815
BstAUI TGTACA 1 cut(s) 987
BstBI TTCGAA 1 cut(s) 123
BstDEI CTNAG 9 cut(s) 113, 1068, 1831, 2049, 2916, 3265, 3343, 3580, 3666
BstDSI CCRYGG 1 cut(s) 1895
BstF5I GGATG 5 cut(s) 277, 982, 1093, 1828, 2341
BstHHI GCGC 3 cut(s) 57, 2497, 2582
BstMAI GTCTC 5 cut(s) 663, 1869, 2529, 3272, 3651
BstNSI RCATGY 7 cut(s) 588, 697, 3793, 3868, 3943, 4018, 4093
BstSFI CTRYAG 3 cut(s) 207, 500, 2409
BstV2I GAAGAC 1 cut(s) 132
BstX2I RGATCY 9 cut(s) 265, 397, 2128, 3188, 3269, 3332, 3395, 3521, 3584
BstYI RGATCY 9 cut(s) 265, 397, 2128, 3188, 3269, 3332, 3395, 3521, 3584
Bsu36I CCTNAGG 2 cut(s) 3343, 3666
BsuI GTATCC 1 cut(s) 1985
BtgI CCRYGG 1 cut(s) 1895
BtgZI GCGATG 3 cut(s) 1284, 2982, 3333
BtrI CACGTC 1 cut(s) 434
BtsCI GGATG 5 cut(s) 277, 982, 1093, 1828, 2341
BtsI GCAGTG 4 cut(s) 2674, 3760, 4342, 4417
BtsIMutI CAGTG 8 cut(s) 166, 2607, 2674, 3405, 3760, 4342, 4417, 4585
BveI ACCTGC 3 cut(s) 2416, 2746, 3020
CaiI CAGNNNCTG 3 cut(s) 206, 1427, 3587
CfoI GCGC 3 cut(s) 57, 2497, 2582
Cfr10I RCCGGY 1 cut(s) 3533
CseI GACGC 2 cut(s) 97, 137
Csp6I GTAC 8 cut(s) 492, 837, 916, 988, 1116, 2373, 2990, 3444
CspAI ACCGGT 1 cut(s) 3533
CspCI CAANNNNNGTGG 2 cut(s) 61, 96
CviQI GTAC 8 cut(s) 492, 837, 916, 988, 1116, 2373, 2990, 3444
DdeI CTNAG 9 cut(s) 113, 1068, 1831, 2049, 2916, 3265, 3343, 3580, 3666
DraI TTTAAA 1 cut(s) 2145
DriI GACNNNNNGTC 2 cut(s) 73, 565
EaeI YGGCCR 4 cut(s) 85, 1468, 4492, 4552
Eam1104I CTCTTC 5 cut(s) 86, 1035, 3124, 3434, 3730
Eam1105I GACNNNNNGTC 2 cut(s) 73, 565
EarI CTCTTC 5 cut(s) 86, 1035, 3124, 3434, 3730
EciI GGCGGA 3 cut(s) 890, 1150, 3168
Eco130I CCWWGG 3 cut(s) 1338, 1895, 4183
Eco147I AGGCCT 1 cut(s) 853
Eco24I GRGCYC 3 cut(s) 703, 1238, 1298
Eco31I GGTCTC 1 cut(s) 3651
Eco47I GGWCC 6 cut(s) 1137, 1274, 1335, 1544, 1667, 1979
Eco57I CTGAAG 4 cut(s) 360, 1404, 2109, 2499
Eco81I CCTNAGG 2 cut(s) 3343, 3666
Eco88I CYCGRG 3 cut(s) 615, 865, 890
EcoO109I RGGNCCY 2 cut(s) 1274, 1544
EcoRI GAATTC 3 cut(s) 2809, 3451, 3634
EcoT14I CCWWGG 3 cut(s) 1338, 1895, 4183
EcoT22I ATGCAT 1 cut(s) 425
EcoT38I GRGCYC 3 cut(s) 703, 1238, 1298
ErhI CCWWGG 3 cut(s) 1338, 1895, 4183
Esp3I CGTCTC 1 cut(s) 1869
FaqI GGGAC 6 cut(s) 1841, 2758, 2882, 3789, 4187, 4289
FauI CCCGC 4 cut(s) 41, 1403, 1884, 3740
FauNDI CATATG 1 cut(s) 1634
FbaI TGATCA 4 cut(s) 873, 2509, 2599, 2686
FokI GGATG 5 cut(s) 284, 989, 1100, 1835, 2348
FriOI GRGCYC 3 cut(s) 703, 1238, 1298
GlaI GCGC 3 cut(s) 56, 2496, 2581
GsaI CCCAGC 8 cut(s) 2205, 2217, 2440, 2527, 2617, 2704, 2745, 3022
GsuI CTGGAG 5 cut(s) 2256, 2292, 2959, 3108, 3132
HgaI GACGC 2 cut(s) 97, 137
HhaI GCGC 3 cut(s) 57, 2497, 2582
Hin1I GRCGYC 3 cut(s) 5, 108, 129
Hin6I GCGC 3 cut(s) 55, 2495, 2580
HinP1I GCGC 3 cut(s) 55, 2495, 2580
HincII GTYRAC 3 cut(s) 475, 2753, 2855
HindII GTYRAC 3 cut(s) 475, 2753, 2855
HindIII AAGCTT 3 cut(s) 1015, 1793, 2039
Hpy166II GTNNAC 8 cut(s) 475, 754, 2753, 2855, 3053, 3104, 3131, 3692
Hpy8I GTNNAC 8 cut(s) 475, 754, 2753, 2855, 3053, 3104, 3131, 3692
Hpy99I CGWCG 6 cut(s) 10, 68, 71, 149, 152, 2022
HpyCH4IV ACGT 4 cut(s) 433, 1394, 1570, 2017
HpyF3I CTNAG 9 cut(s) 113, 1068, 1831, 2049, 2916, 3265, 3343, 3580, 3666
HpySE526I ACGT 4 cut(s) 433, 1394, 1570, 2017
Hsp92I GRCGYC 3 cut(s) 5, 108, 129
HspAI GCGC 3 cut(s) 55, 2495, 2580
Kpn2I TCCGGA 1 cut(s) 2131
KpnI GGTACC 1 cut(s) 495
Ksp22I TGATCA 4 cut(s) 873, 2509, 2599, 2686
LguI GCTCTTC 1 cut(s) 3124
LweI GCATC 7 cut(s) 450, 2252, 2288, 2440, 2806, 2959, 3583
MaeII ACGT 4 cut(s) 433, 1394, 1570, 2017
MaeIII GTNAC 8 cut(s) 1514, 1913, 2068, 2483, 2660, 3142, 3193, 3401
MfeI CAATTG 1 cut(s) 1950
MflI RGATCY 9 cut(s) 265, 397, 2128, 3188, 3269, 3332, 3395, 3521, 3584
MmeI TCCRAC 6 cut(s) 168, 409, 1641, 1653, 3554, 3611
Mph1103I ATGCAT 1 cut(s) 425
MroI TCCGGA 1 cut(s) 2131
MroXI GAANNNNTTC 3 cut(s) 2104, 2809, 3003
MslI CAYNNNNRTG 1 cut(s) 2682
MspA1I CMGCKG 5 cut(s) 134, 2996, 3086, 3149, 4218
MunI CAATTG 1 cut(s) 1950
Mva1269I GAATGC 3 cut(s) 442, 2236, 2272
NciI CCSGG 6 cut(s) 105, 2251, 3784, 3934, 4009, 4159
NcoI CCATGG 1 cut(s) 1895
NdeI CATATG 1 cut(s) 1634
NmuCI GTSAC 4 cut(s) 2483, 2660, 3193, 3401
NsiI ATGCAT 1 cut(s) 425
NspI RCATGY 7 cut(s) 588, 697, 3793, 3868, 3943, 4018, 4093
NspV TTCGAA 1 cut(s) 123
PaeR7I CTCGAG 1 cut(s) 890
PaqCI CACCTGC 2 cut(s) 2416, 3020
PasI CCCWGGG 9 cut(s) 224, 3808, 3883, 3958, 4033, 4108, 4285, 4390, 4465
PceI AGGCCT 1 cut(s) 853
PciI ACATGT 5 cut(s) 3789, 3864, 3939, 4014, 4089
PciSI GCTCTTC 1 cut(s) 3124
PcsI WCGNNNNNNNCGW 3 cut(s) 135, 153, 1667
PctI GAATGC 3 cut(s) 442, 2236, 2272
PdmI GAANNNNTTC 3 cut(s) 2104, 2809, 3003
PinAI ACCGGT 1 cut(s) 3533
PpuMI RGGWCCY 2 cut(s) 1274, 1544
PscI ACATGT 5 cut(s) 3789, 3864, 3939, 4014, 4089
PshBI ATTAAT 2 cut(s) 576, 2319
Psp5II RGGWCCY 2 cut(s) 1274, 1544
PspFI CCCAGC 8 cut(s) 2201, 2213, 2436, 2523, 2613, 2700, 2741, 3018
PspOMI GGGCCC 1 cut(s) 699
PspPPI RGGWCCY 2 cut(s) 1274, 1544
PspXI VCTCGAGB 1 cut(s) 890
PsrI GAACNNNNNNTAC 2 cut(s) 612, 644
PstI CTGCAG 1 cut(s) 2413
PstNI CAGNNNCTG 3 cut(s) 206, 1427, 3587
PsuI RGATCY 9 cut(s) 265, 397, 2128, 3188, 3269, 3332, 3395, 3521, 3584
PvuII CAGCTG 2 cut(s) 2996, 3086
RsaI GTAC 8 cut(s) 493, 838, 917, 989, 1117, 2374, 2991, 3445
RsaNI GTAC 8 cut(s) 492, 837, 916, 988, 1116, 2373, 2990, 3444
RseI CAYNNNNRTG 1 cut(s) 2682
SapI GCTCTTC 1 cut(s) 3124
ScaI AGTACT 1 cut(s) 3445
SfaNI GCATC 7 cut(s) 450, 2252, 2288, 2440, 2806, 2959, 3583
SfcI CTRYAG 3 cut(s) 207, 500, 2409
Sfr274I CTCGAG 1 cut(s) 890
SfuI TTCGAA 1 cut(s) 123
SgrAI CRCCGGYG 1 cut(s) 3533
SinI GGWCC 6 cut(s) 1137, 1274, 1335, 1544, 1667, 1979
SlaI CTCGAG 1 cut(s) 890
SmiMI CAYNNNNRTG 1 cut(s) 2682
SmlI CTYRAG 1 cut(s) 890
SmoI CTYRAG 1 cut(s) 890
SseBI AGGCCT 1 cut(s) 853
StuI AGGCCT 1 cut(s) 853
StyI CCWWGG 3 cut(s) 1338, 1895, 4183
TaiI ACGT 4 cut(s) 436, 1397, 1573, 2020
TatI WGTACW 4 cut(s) 915, 987, 2372, 3443
TauI GCSGC 2 cut(s) 29, 134
TscAI CASTG 8 cut(s) 166, 2614, 2674, 3412, 3760, 4342, 4417, 4585
TseFI GTSAC 4 cut(s) 2483, 2660, 3193, 3401
Tsp45I GTSAC 4 cut(s) 2483, 2660, 3193, 3401
TspGWI ACGGA 4 cut(s) 1961, 1971, 2183, 3183
TspRI CASTG 8 cut(s) 166, 2614, 2674, 3412, 3760, 4342, 4417, 4585
VpaK11BI GGWCC 6 cut(s) 1137, 1274, 1335, 1544, 1667, 1979
VspI ATTAAT 2 cut(s) 576, 2319
XceI RCATGY 7 cut(s) 588, 697, 3793, 3868, 3943, 4018, 4093
XcmI CCANNNNNNNNNTGG 2 cut(s) 1745, 1893
XhoI CTCGAG 1 cut(s) 890
XmnI GAANNNNTTC 3 cut(s) 2104, 2809, 3003
ZrmI AGTACT 1 cut(s) 3445
Zsp2I ATGCAT 1 cut(s) 425
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.