Rh1DG298300

Transcription elongation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0012966
Physical Location & Seq
Reverse (-)
52372723 .. 52380625
7903 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG298300.1

Sequence Viewer

Length: 3003 bp
ATGGCCTCGAAGGAAAAAGGAAAGGCGATCGCCACAAGCGGATCGTCCGCCGCCGGCAAGCGGAAGCCCGGCGATGATGACAAGTCCGGCGGCGGCAGTCGGAAGCGGAGAAACCCCGGCGTTCTCCAGTTCTTCGAGCACTCCGCCGCCGAAGCTGACGGTTCGGACGACGACAGCGACTTGGACGATTTTATGGAACAAGAACTTGAAACAGAACCTATAGTCAAGAGTGAACCGGGGAAAGCCCATAACCTTCCATTTGTTCCCAAAGAGGAGGATATCGATGGGGAAGAATTTGAAAGAATGATGGAAGAACGATACAGGACTGGTTCTACATATGTTACCTATGCTGAAGATAATTATGAAAACAAAAGATCGGTTGATGGAATTGTGCTTGAGCCTTCTGCTAAAGATCCTGTTATCTGGAAAGTAAAGTGTGCGGTTGGACGCGAGAGACACTCAGCTTTCTGTATGATGCAGAAGTTTGTTGACTTACGATCAATGGGTACCAAGCTGCAGATAATATCTGCATTTGCTGTTGATCACATCAAGGGTTTTGTTTTCATTGAAGCTGATAAGCTATGTGACGTTCAGGAGGCATGTAAAGGACTTTGTAATATATTTTTATCCCGAGTAACGCCAGTTCCCAAAAATGAAGCTCCTCATTTACTGGCTCCTCGGACTAGAAACAGTGAAATTACTGTGGGTACATGGGCCCGTGTGAAGAGTGGGAACTACAAGGGTGACTTGGCACAGGTTGTGGCTGTGAACGAAAAGAAAAAAGCAACAGTAAAGCTGATACCAAGAATTGATCTACAAGCAATGGCTGCAAAATTTGGTGGGGGAGTTACTGGAAAGAAACTGCCTACTCCAGCACCGAGATTGATCAGCACAAGCGAACTTGAGGAGTTCCGCCCTCTCATTCAGGACAGGAAGGATAAGGATACCGGATTGCGTTTTCTCTGTCTTGATGGTTTGCTCCTTAAGGATGGATATTTATACAAAAAAATGCCATTAGATTCATTAAATTGCTGGGGTGTCATGCCATCAGAAGAGGAGATACTGAAATTCAAGCCTTCTGAGAACAATGAATCTGCTGATCTGGAGTGGCTTTCACAGCTTTATGGTGACAATAAGAAAAGGAAGAGCATCGGCAGTGATAAAGGGGATGGCAAAGGAGAAGGTTCAGCAGGGTGTGGTGGGAAAGGAGAAGGTTCATCAAAGGGTAACGGGAAAGGAGAAGGTCCTTCAGGTGGGAAAGAAGGTTCTTCAGGGTTTACCGGGAAAGGAGAAGGTTCTTCAGGGTGTGGTGGGAATCTCTATGAGCTGTATGATCTTGTTTGTTTCGGCAAGAAAGATTTTGGTCTTGTGCTAGGCATTGAGAAAGATGATACTTACAAGATTCTGAAAGAGGGTGTGGAAGGATCTACTGTTGTGACAGTTCAGCAGAATGAGATAAAGAATGTACTTTCTGATGTAAAATTTACAGCTTTAGATCAGCGCCTGAAGCCCATTCGCGTCAATGATACAGTTAAAGTTTTGGAAGGACCATTGAAGGATAGGCAAGGAATAGTGAAGCAAGTCTACAGAGGCACAATATTCATGTTTGATGAGAATGAGACAGAAAATGGTGGTTATTTCTGCTCAAAATCTCAAATGTGTGAGAAAATCAAGCTTTCCATTGACGTTATCCCAGAAAAGGATGGGGATTCGGGTGCTTTCGGTTTTGAAGATTTCACATCATCACCTAAATCCCCTCTATCGCCAAAGAAGCCTTGGCTAGGAAGGGTGAATAACTTCAATCAGGGTGATACTGACGGAATGTTCTCAATCGGTCAAACTTTAAGAATTTGTGTTGGTCCATTAAAGGGATACCTCTGTCGTGTCTTGGCCATACGTCGTTCTGATATTACAGTTAAGCTTGATTCTCAGCAAAGGGTTCTTACAGTTAAAGCTGAGCATCTTACAGAGGTTCGTGTAAAGAGCGCTGCTATGCTTAGTGAGGATCTGGAGTCCAGCTCTCTTAAACCATTTGATTTACTTGGAACTGAAGGAGGCTCTAAAGATTGGACAGATGGAGCTGGGACATCAGCCGGGGGTGATGGGTGGAATGCTGGAGGGTCATCTGGTGAAAGGAATGCTTGGCCTAGTTTCTCTGCCTCGGGCAATTCGCTTCAGCCTGAGTCCAGCTCTGCGAATCCTTTTGAATCTGATGGAAATGGTGCTAAAAAAGAGGATAGTTCTTGGGAGAGGAACGTGGCTCCAAATAACAACTCATCCTGGGGTGTTGCAGCAGCAGAAAACAAGGATCAAGTTACTGGCTGGGGAAAAAGCAACTCTTGGGGCGCTAAAGTTGGAGGTGACAGCAGTCTGTCAGATACTTTGCAAAAAGCATCAGAACCTGCCAGTTCATCCTGGGGTGTTGCAGCAGCAGTAGATAACAAGGATCAAGGTACTGGCTGGGGAAAAAGTGACTCTTGGGGCGCTAAAGTTGGAGGTGACAGCACTTCGTCGGATACTTGGCAAAAAGCATCAGCGCCTGCTAGTTCATCCTGGGGTGTTGCGGCAGCAATAGATAAGAAGGATCAAGGTACTGGCTGGGGTGCTAAGACTGGATGTGACGGCACTGCGTCAGATAGTTGGAAAAAAGCATCAGAACCTGCTAGTAATTCGTCCTGGGGTGTTGCAGCAGCAGCAGATAACAAGGATCAAGGTTCCGTCTGGGGCGGTAATGGTAGTCAAAAACCAAGTGTTGCAGATGATAGTGGTGACAAAGGTTCTGCTTGGAATAAGCCAGCTGAAGGTTCTTGGAGTAAACAAACTGGAGGATCCTCATGGGGTAAGCAAGCCGATTGGAGCACTAAAGAGGGATCAAGTACTGGTGATGCAGGGTGGAAAAGTTCAACACCTTCAGTAGAGAATCAGACTGGGAGCTGGGGTAATGCAGGTGGCTGGGCAAAAGCTCAAGGCATATCTGGAAGATGGAGGCTCTACCTGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006351 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006368 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0006996 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016043 GO:0016070 GO:0016071 GO:0016246 GO:0016441 GO:0016458 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0030422 GO:0031047 GO:0031050 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032044 GO:0032259 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044728 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0071840 GO:0080090 GO:0080188 GO:0090304 GO:0097159 GO:0097659 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901698 GO:1901699 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Spt5-NGN

Protein Analysis

1000

Amino Acids

106.94

Weight (kDa)

5.59

Isoelectric Point (pI)

34.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Spt5-NGN PF03439 141 - 224 1.1e-19 Early transcription elongation factor of RNA pol II, NGN section
KOW1_SPT5 PF23042 235 - 357 1.8e-25 Transcription elongation factor SPT5, KOW1 domain
KOWx_SPT5 PF23037 438 - 488 2.1e-10 Transcription elongation factor SPT5, KOWx domain
KOW4_SPT5 PF23291 497 - 551 2.5e-22 Transcription elongation factor SPT5, fourth KOW domain
KOW5_SPT5 PF23290 611 - 655 3.2e-06 Transcription elongation factor SPT5, fifth KOW domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2936
Acc36I ACCTGC 3 cut(s) 2410, 2668, 2936
Acc65I GGTACC 1 cut(s) 506
AccB1I GGYRCC 1 cut(s) 506
AccI GTMKAC 1 cut(s) 1584
AccII CGCG 2 cut(s) 450, 1518
AcoI YGGCCR 1 cut(s) 1890
AcsI RAATTY 5 cut(s) 293, 833, 1067, 1481, 1848
AcuI CTGAAG 9 cut(s) 372, 1233, 1254, 1284, 1526, 2070, 2159, 2820, 2895
AfaI GTAC 6 cut(s) 508, 709, 1467, 2455, 2593, 2878
AfeI AGCGCT 1 cut(s) 1987
AfiI CCNNNNNNNGG 6 cut(s) 60, 1253, 1699, 1781, 1868, 2801
AflII CTTAAG 1 cut(s) 983
AgsI TTSAA 9 cut(s) 209, 299, 569, 1072, 1555, 1730, 1801, 2207, 2904
AjnI CCWGG 5 cut(s) 2279, 2414, 2552, 2675, 2994
AloI GAACNNNNNNTCC 2 cut(s) 1249, 1281
Alw21I GWGCWC 2 cut(s) 141, 2861
Alw26I GTCTC 2 cut(s) 448, 1613
AlwNI CAGNNNCTG 4 cut(s) 1504, 2402, 2540, 2660
Ama87I CYCGRG 2 cut(s) 630, 2161
Aor51HI AGCGCT 1 cut(s) 1987
AoxI GGCC 4 cut(s) 3, 714, 1890, 2144
ApaI GGGCCC 1 cut(s) 718
ApoI RAATTY 5 cut(s) 293, 833, 1067, 1481, 1848
AsiSI GCGATCGC 1 cut(s) 30
Asp700I GAANNNNTTC 1 cut(s) 1796
Asp718I GGTACC 1 cut(s) 506
AspLEI GCGC 5 cut(s) 1503, 1988, 2348, 2486, 2539
AspS9I GGNCC 5 cut(s) 714, 715, 1244, 1547, 1859
AsuC2I CCSGG 5 cut(s) 69, 117, 237, 1282, 2095
AvaI CYCGRG 2 cut(s) 630, 2161
AvaII GGWCC 3 cut(s) 1244, 1547, 1859
BaeGI GKGCMC 1 cut(s) 718
BaeI ACNNNNGTAYC 2 cut(s) 1864, 1897
BalI TGGCCA 1 cut(s) 1892
BamHI GGATCC 1 cut(s) 2828
BanI GGYRCC 1 cut(s) 506
BanII GRGCYC 1 cut(s) 718
BarI GAAGNNNNNNTAC 4 cut(s) 1044, 1076, 1568, 1600
Bbv12I GWGCWC 2 cut(s) 141, 2861
BceAI ACGGC 1 cut(s) 2638
BciT130I CCWGG 5 cut(s) 2281, 2416, 2554, 2677, 2996
BciVI GTATCC 3 cut(s) 937, 1865, 2509
BclI TGATCA 2 cut(s) 541, 885
BcnI CCSGG 5 cut(s) 69, 117, 237, 1282, 2095
BcoDI GTCTC 2 cut(s) 448, 1613
BfaI CTAG 6 cut(s) 684, 1373, 1781, 2148, 2544, 2664
BfmI CTRYAG 3 cut(s) 219, 515, 1585
BfoI RGCGCY 5 cut(s) 1504, 1989, 2349, 2487, 2540
BfrI CTTAAG 1 cut(s) 983
BfuAI ACCTGC 3 cut(s) 2410, 2668, 2936
BfuI GTATCC 3 cut(s) 937, 1865, 2509
BlpI GCTNAGC 1 cut(s) 1956
BmcAI AGTACT 1 cut(s) 2878
Bme18I GGWCC 3 cut(s) 1244, 1547, 1859
BmeT110I CYCGRG 2 cut(s) 630, 2161
BmgT120I GGNCC 5 cut(s) 714, 715, 1244, 1547, 1859
BmiI GGNNCC 6 cut(s) 508, 675, 716, 2262, 2716, 2830
BmrI ACTGGG 1 cut(s) 2937
BmsI GCATC 7 cut(s) 465, 1158, 1969, 2402, 2540, 2660, 2875
BmuI ACTGGG 1 cut(s) 2937
BoxI GACNNNNGTC 1 cut(s) 2367
BplI GAGNNNNNCTC 6 cut(s) 443, 475, 2003, 2035, 2174, 2206
BpmI CTGGAG 6 cut(s) 110, 855, 1124, 2030, 2136, 2844
Bpu1102I GCTNAGC 1 cut(s) 1956
BpuEI CTTGAG 3 cut(s) 416, 923, 2949
BpuMI CCSGG 5 cut(s) 69, 117, 237, 1282, 2095
Bsa29I ATCGAT 1 cut(s) 282
BsaWI WCCGGW 1 cut(s) 947
BsaXI ACNNNNNCTCC 2 cut(s) 2923, 2953
Bsc4I CCNNNNNNNGG 6 cut(s) 60, 1253, 1699, 1781, 1868, 2801
Bse118I RCCGGY 1 cut(s) 53
Bse3DI GCAATG 1 cut(s) 828
BseBI CCWGG 5 cut(s) 2281, 2416, 2554, 2677, 2996
BseCI ATCGAT 1 cut(s) 282
BseGI GGATG 7 cut(s) 994, 1174, 1708, 2276, 2411, 2549, 2621
BseLI CCNNNNNNNGG 6 cut(s) 60, 1253, 1699, 1781, 1868, 2801
BseMI GCAATG 1 cut(s) 828
BseMII CTCAG 5 cut(s) 474, 1071, 1943, 1947, 2172
BseRI GAGGAG 5 cut(s) 287, 651, 666, 920, 1070
BseSI GKGCMC 1 cut(s) 718
BseYI CCCAGC 7 cut(s) 1032, 2081, 2322, 2460, 2598, 2934, 2952
Bsh1236I CGCG 2 cut(s) 450, 1518
Bsh1285I CGRYCG 1 cut(s) 30
BshFI GGCC 4 cut(s) 5, 716, 1892, 2146
BshNI GGYRCC 1 cut(s) 506
BshVI ATCGAT 1 cut(s) 282
BsiEI CGRYCG 1 cut(s) 30
BsiHKAI GWGCWC 2 cut(s) 141, 2861
BsiHKCI CYCGRG 2 cut(s) 630, 2161
BsiSI CCGG 8 cut(s) 54, 69, 87, 117, 236, 948, 1281, 2094
BslFI GGGAC 1 cut(s) 2098
BslI CCNNNNNNNGG 6 cut(s) 60, 1253, 1699, 1781, 1868, 2801
BsmAI GTCTC 2 cut(s) 448, 1613
BsmFI GGGAC 1 cut(s) 2098
BsmI GAATGC 2 cut(s) 2116, 2143
BsnI GGCC 4 cut(s) 5, 716, 1892, 2146
BsoBI CYCGRG 2 cut(s) 630, 2161
Bsp120I GGGCCC 1 cut(s) 714
Bsp1286I GDGCHC 3 cut(s) 141, 718, 2861
Bsp1720I GCTNAGC 1 cut(s) 1956
BspANI GGCC 4 cut(s) 5, 716, 1892, 2146
BspCNI CTCAG 5 cut(s) 473, 1072, 1942, 1948, 2173
BspDI ATCGAT 1 cut(s) 282
BspFNI CGCG 2 cut(s) 450, 1518
BspLI GGNNCC 6 cut(s) 508, 675, 716, 2262, 2716, 2830
BspMAI CTGCAG 1 cut(s) 519
BspMI ACCTGC 3 cut(s) 2410, 2668, 2936
BspQI GCTCTTC 1 cut(s) 1139
BspT107I GGYRCC 1 cut(s) 506
BspTI CTTAAG 1 cut(s) 983
BsrDI GCAATG 1 cut(s) 828
BsrFI RCCGGY 1 cut(s) 53
BssAI RCCGGY 1 cut(s) 53
BssT1I CCWWGG 1 cut(s) 1775
Bst2UI CCWGG 5 cut(s) 2281, 2416, 2554, 2677, 2996
Bst4CI ACNGT 9 cut(s) 161, 692, 703, 790, 1432, 1441, 1531, 1915, 1948
Bst6I CTCTTC 3 cut(s) 719, 1047, 1139
BstAFI CTTAAG 1 cut(s) 983
BstAPI GCANNNNNTGC 1 cut(s) 827
BstC8I GCNNGC 5 cut(s) 55, 59, 2541, 2796, 2847
BstDEI CTNAG 7 cut(s) 460, 1080, 1929, 1956, 1997, 2181, 2607
BstENI CCTNNNNNAGG 1 cut(s) 1779
BstF5I GGATG 7 cut(s) 994, 1174, 1708, 2276, 2411, 2549, 2621
BstFNI CGCG 2 cut(s) 450, 1518
BstH2I RGCGCY 5 cut(s) 1504, 1989, 2349, 2487, 2540
BstHHI GCGC 5 cut(s) 1503, 1988, 2348, 2486, 2539
BstMAI GTCTC 2 cut(s) 448, 1613
BstMCI CGRYCG 1 cut(s) 30
BstMWI GCNNNNNNNGC 6 cut(s) 152, 827, 1117, 1507, 1771, 2693
BstNI CCWGG 5 cut(s) 2281, 2416, 2554, 2677, 2996
BstNSI RCATGY 1 cut(s) 603
BstPAI GACNNNNGTC 1 cut(s) 2367
BstSFI CTRYAG 3 cut(s) 219, 515, 1585
BstSLI GKGCMC 1 cut(s) 718
BstUI CGCG 2 cut(s) 450, 1518
BstX2I RGATCY 4 cut(s) 412, 1424, 2005, 2828
BstYI RGATCY 4 cut(s) 412, 1424, 2005, 2828
Bsu15I ATCGAT 1 cut(s) 282
BsuI GTATCC 3 cut(s) 937, 1865, 2509
BsuRI GGCC 4 cut(s) 5, 716, 1892, 2146
BsuTUI ATCGAT 1 cut(s) 282
BtgZI GCGATG 1 cut(s) 87
BtsCI GGATG 7 cut(s) 994, 1174, 1708, 2276, 2411, 2549, 2621
BtsI GCAGTG 2 cut(s) 1162, 2625
BtsIMutI CAGTG 3 cut(s) 697, 1162, 2625
BveI ACCTGC 3 cut(s) 2410, 2668, 2936
Cac8I GCNNGC 5 cut(s) 55, 59, 2541, 2796, 2847
CaiI CAGNNNCTG 4 cut(s) 1504, 2402, 2540, 2660
CfoI GCGC 5 cut(s) 1503, 1988, 2348, 2486, 2539
Cfr10I RCCGGY 1 cut(s) 53
Cfr13I GGNCC 5 cut(s) 714, 715, 1244, 1547, 1859
ClaI ATCGAT 1 cut(s) 282
CseI GACGC 3 cut(s) 456, 1507, 2619
Csp6I GTAC 6 cut(s) 507, 708, 1466, 2454, 2592, 2877
CviAII CATG 5 cut(s) 600, 711, 1042, 1603, 2835
CviQI GTAC 6 cut(s) 507, 708, 1466, 2454, 2592, 2877
DdeI CTNAG 7 cut(s) 460, 1080, 1929, 1956, 1997, 2181, 2607
EaeI YGGCCR 1 cut(s) 1890
Eam1104I CTCTTC 3 cut(s) 719, 1047, 1139
EarI CTCTTC 3 cut(s) 719, 1047, 1139
EciI GGCGGA 3 cut(s) 37, 133, 902
Eco130I CCWWGG 1 cut(s) 1775
Eco24I GRGCYC 1 cut(s) 718
Eco32I GATATC 1 cut(s) 280
Eco47I GGWCC 3 cut(s) 1244, 1547, 1859
Eco47III AGCGCT 1 cut(s) 1987
Eco57I CTGAAG 9 cut(s) 372, 1233, 1254, 1284, 1526, 2070, 2159, 2820, 2895
Eco88I CYCGRG 2 cut(s) 630, 2161
EcoNI CCTNNNNNAGG 1 cut(s) 1779
EcoO109I RGGNCCY 1 cut(s) 1244
EcoRII CCWGG 5 cut(s) 2279, 2414, 2552, 2675, 2994
EcoRV GATATC 1 cut(s) 280
EcoT14I CCWWGG 1 cut(s) 1775
EcoT38I GRGCYC 1 cut(s) 718
ErhI CCWWGG 1 cut(s) 1775
FaeI CATG 5 cut(s) 603, 714, 1045, 1606, 2838
FalI AAGNNNNNCTT 8 cut(s) 731, 763, 2125, 2157, 2323, 2355, 2461, 2493
FaqI GGGAC 1 cut(s) 2098
FatI CATG 5 cut(s) 599, 710, 1041, 1602, 2834
FauNDI CATATG 1 cut(s) 337
FbaI TGATCA 2 cut(s) 541, 885
FblI GTMKAC 1 cut(s) 1584
FokI GGATG 7 cut(s) 1001, 1181, 1715, 2263, 2398, 2536, 2628
FriOI GRGCYC 1 cut(s) 718
FspBI CTAG 6 cut(s) 684, 1373, 1781, 2148, 2544, 2664
GlaI GCGC 5 cut(s) 1502, 1987, 2347, 2485, 2538
GsaI CCCAGC 7 cut(s) 1036, 2085, 2326, 2464, 2602, 2938, 2956
GsuI CTGGAG 6 cut(s) 110, 855, 1124, 2030, 2136, 2844
HaeII RGCGCY 5 cut(s) 1504, 1989, 2349, 2487, 2540
HaeIII GGCC 4 cut(s) 5, 716, 1892, 2146
HapII CCGG 8 cut(s) 54, 69, 87, 117, 236, 948, 1281, 2094
HgaI GACGC 3 cut(s) 456, 1507, 2619
HhaI GCGC 5 cut(s) 1503, 1988, 2348, 2486, 2539
Hin1II CATG 5 cut(s) 603, 714, 1045, 1606, 2838
Hin6I GCGC 5 cut(s) 1501, 1986, 2346, 2484, 2537
HinP1I GCGC 5 cut(s) 1501, 1986, 2346, 2484, 2537
HincII GTYRAC 1 cut(s) 490
HindII GTYRAC 1 cut(s) 490
HindIII AAGCTT 2 cut(s) 1673, 1919
HpaII CCGG 8 cut(s) 54, 69, 87, 117, 236, 948, 1281, 2094
Hpy166II GTNNAC 6 cut(s) 233, 490, 769, 1278, 1585, 2816
Hpy188III TCNNGA 9 cut(s) 226, 424, 593, 630, 926, 968, 1103, 2009, 2976
Hpy8I GTNNAC 6 cut(s) 233, 490, 769, 1278, 1585, 2816
Hpy99I CGWCG 3 cut(s) 173, 1902, 2515
HpyCH4III ACNGT 9 cut(s) 161, 692, 703, 790, 1432, 1441, 1531, 1915, 1948
HpyCH4IV ACGT 4 cut(s) 588, 1686, 1897, 2256
HpyF10VI GCNNNNNNNGC 6 cut(s) 152, 827, 1117, 1507, 1771, 2693
HpyF3I CTNAG 7 cut(s) 460, 1080, 1929, 1956, 1997, 2181, 2607
HpySE526I ACGT 4 cut(s) 588, 1686, 1897, 2256
Hsp92II CATG 5 cut(s) 603, 714, 1045, 1606, 2838
HspAI GCGC 5 cut(s) 1501, 1986, 2346, 2484, 2537
KpnI GGTACC 1 cut(s) 510
KroI GCCGGC 1 cut(s) 53
KroNI GCCGGC 1 cut(s) 55
Ksp22I TGATCA 2 cut(s) 541, 885
LguI GCTCTTC 1 cut(s) 1139
LmnI GCTCC 7 cut(s) 664, 679, 984, 2078, 2266, 2856, 2931
LweI GCATC 7 cut(s) 465, 1158, 1969, 2402, 2540, 2660, 2875
MaeI CTAG 6 cut(s) 684, 1373, 1781, 2148, 2544, 2664
MaeII ACGT 4 cut(s) 588, 1686, 1897, 2256
MflI RGATCY 4 cut(s) 412, 1424, 2005, 2828
MhlI GDGCHC 3 cut(s) 141, 718, 2861
MlsI TGGCCA 1 cut(s) 1892
MluNI TGGCCA 1 cut(s) 1892
MlyI GAGTC 3 cut(s) 2021, 2192, 2468
MmeI TCCRAC 6 cut(s) 80, 424, 2335, 2473, 2493, 2621
Mox20I TGGCCA 1 cut(s) 1892
MroNI GCCGGC 1 cut(s) 53
MroXI GAANNNNTTC 1 cut(s) 1796
MscI TGGCCA 1 cut(s) 1892
MseI TTAA 8 cut(s) 984, 1025, 1533, 1844, 1865, 1917, 1950, 2025
Msp20I TGGCCA 1 cut(s) 1892
MspA1I CMGCKG 1 cut(s) 2798
MspCI CTTAAG 1 cut(s) 983
MspI CCGG 8 cut(s) 54, 69, 87, 117, 236, 948, 1281, 2094
Mva1269I GAATGC 2 cut(s) 2116, 2143
MvaI CCWGG 5 cut(s) 2281, 2416, 2554, 2677, 2996
MvnI CGCG 2 cut(s) 450, 1518
MwoI GCNNNNNNNGC 6 cut(s) 152, 827, 1117, 1507, 1771, 2693
NaeI GCCGGC 1 cut(s) 55
NciI CCSGG 5 cut(s) 69, 117, 237, 1282, 2095
NdeI CATATG 1 cut(s) 337
NgoMIV GCCGGC 1 cut(s) 53
NlaIII CATG 5 cut(s) 603, 714, 1045, 1606, 2838
NlaIV GGNNCC 6 cut(s) 508, 675, 716, 2262, 2716, 2830
NmuCI GTSAC 9 cut(s) 584, 743, 1127, 1435, 2360, 2471, 2498, 2618, 2768
NspI RCATGY 1 cut(s) 603
PaqCI CACCTGC 1 cut(s) 2936
PciSI GCTCTTC 1 cut(s) 1139
PcsI WCGNNNNNNNCGW 2 cut(s) 165, 174
PctI GAATGC 2 cut(s) 2116, 2143
PdiI GCCGGC 1 cut(s) 55
PdmI GAANNNNTTC 1 cut(s) 1796
PfeI GAWTC 9 cut(s) 1019, 1091, 1315, 1402, 1709, 1925, 2197, 2207, 2920
Ple19I CGATCG 1 cut(s) 30
PleI GAGTC 3 cut(s) 2020, 2191, 2468
PpsI GAGTC 3 cut(s) 2020, 2191, 2468
PpuMI RGGWCCY 1 cut(s) 1244
PshAI GACNNNNGTC 1 cut(s) 2367
Psp5II RGGWCCY 1 cut(s) 1244
Psp6I CCWGG 5 cut(s) 2279, 2414, 2552, 2675, 2994
PspFI CCCAGC 7 cut(s) 1032, 2081, 2322, 2460, 2598, 2934, 2952
PspGI CCWGG 5 cut(s) 2279, 2414, 2552, 2675, 2994
PspN4I GGNNCC 6 cut(s) 508, 675, 716, 2262, 2716, 2830
PspOMI GGGCCC 1 cut(s) 714
PspPI GGNCC 5 cut(s) 714, 715, 1244, 1547, 1859
PspPPI RGGWCCY 1 cut(s) 1244
PsrI GAACNNNNNNTAC 2 cut(s) 627, 659
PstI CTGCAG 1 cut(s) 519
PstNI CAGNNNCTG 4 cut(s) 1504, 2402, 2540, 2660
PsuI RGATCY 4 cut(s) 412, 1424, 2005, 2828
PvuI CGATCG 1 cut(s) 30
PvuII CAGCTG 1 cut(s) 2798
RgaI GCGATCGC 1 cut(s) 30
RsaI GTAC 6 cut(s) 508, 709, 1467, 2455, 2593, 2878
RsaNI GTAC 6 cut(s) 507, 708, 1466, 2454, 2592, 2877
SapI GCTCTTC 1 cut(s) 1139
SaqAI TTAA 8 cut(s) 984, 1025, 1533, 1844, 1865, 1917, 1950, 2025
Sau96I GGNCC 5 cut(s) 714, 715, 1244, 1547, 1859
ScaI AGTACT 1 cut(s) 2878
SchI GAGTC 3 cut(s) 2021, 2192, 2468
SduI GDGCHC 3 cut(s) 141, 718, 2861
SfaAI GCGATCGC 1 cut(s) 30
SfaNI GCATC 7 cut(s) 465, 1158, 1969, 2402, 2540, 2660, 2875
SfcI CTRYAG 3 cut(s) 219, 515, 1585
SgfI GCGATCGC 1 cut(s) 30
SinI GGWCC 3 cut(s) 1244, 1547, 1859
SmlI CTYRAG 4 cut(s) 395, 902, 983, 2964
SmoI CTYRAG 4 cut(s) 395, 902, 983, 2964
SspI AATATT 1 cut(s) 1599
SspMI CTAG 6 cut(s) 684, 1373, 1781, 2148, 2544, 2664
StyI CCWWGG 1 cut(s) 1775
TaaI ACNGT 9 cut(s) 161, 692, 703, 790, 1432, 1441, 1531, 1915, 1948
TaiI ACGT 4 cut(s) 591, 1689, 1900, 2259
TaqI TCGA 3 cut(s) 8, 135, 282
TaqII GACCGA 1 cut(s) 1823
TatI WGTACW 2 cut(s) 1465, 2876
TauI GCSGC 5 cut(s) 53, 93, 96, 149, 2567
TfiI GAWTC 9 cut(s) 1019, 1091, 1315, 1402, 1709, 1925, 2197, 2207, 2920
Tru1I TTAA 8 cut(s) 984, 1025, 1533, 1844, 1865, 1917, 1950, 2025
Tru9I TTAA 8 cut(s) 984, 1025, 1533, 1844, 1865, 1917, 1950, 2025
TscAI CASTG 3 cut(s) 697, 1162, 2632
TseFI GTSAC 9 cut(s) 584, 743, 1127, 1435, 2360, 2471, 2498, 2618, 2768
Tsp45I GTSAC 9 cut(s) 584, 743, 1127, 1435, 2360, 2471, 2498, 2618, 2768
TspDTI ATGAA 9 cut(s) 378, 553, 669, 1011, 1104, 1206, 1591, 2400, 2538
TspGWI ACGGA 2 cut(s) 1833, 2707
TspRI CASTG 3 cut(s) 697, 1162, 2632
Vha464I CTTAAG 1 cut(s) 983
VpaK11BI GGWCC 3 cut(s) 1244, 1547, 1859
XagI CCTNNNNNAGG 1 cut(s) 1779
XapI RAATTY 5 cut(s) 293, 833, 1067, 1481, 1848
XceI RCATGY 1 cut(s) 603
XcmI CCANNNNNNNNNTGG 1 cut(s) 1773
XmiI GTMKAC 1 cut(s) 1584
XmnI GAANNNNTTC 1 cut(s) 1796
XspI CTAG 6 cut(s) 684, 1373, 1781, 2148, 2544, 2664
ZrmI AGTACT 1 cut(s) 2878
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.