Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0006501

Physical Location & Seq

Genomic Coordinates Reverse (-)

1202967 .. 1207946

Gene Structure 4980 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G04310.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1557 bp

ATGGTGGCTCATGAGAGGAGGATCCATAATCTTCAAAAACCAACGTGCATTTGCATTATCTGGTTTTGCCTCTTGGTTTCTCTCTCTCACCATGGAAGAGCATCATCAACCTCAGCATCTATCTTCAATCTTTCTCTCCCACACCAACACCCATTCCCTGAACATGTTGTTCTTAATGTTCAAAGAAAACTCAACGATTCTCTCTCAAGAAGACAACTTCTCACTTACCAACAAGACGACGGCACCACGGCGTCGTCACCAATACCTTCTTGCATCACCGGAAACCCAATCGACGATTGTTGGCGCTGCGACCCAAACTGGTCGGCAAACCGCCAAAGACTCGCCGATTGCTCAATTGGTTTCGGACAAGGAACACTCGGAGGAAAAGGCGGTCAGTTTTACCTCGTCACCGATTCCTCCGACAACGACGCAGCGAATCCAATTCCCGGAACTCTTCGACACGCCGTGATTCAGCCAGAACCACTATGGATCATCTTCTCCAGCGACATGGGAATCAAACTCAAACACGAGCTCATCATCGGAAGCTACAAGACAATCGATGGAAGAGGCACGAATATCCAAATCACCGGCCATGGTTGCCTCACGATTCAGCAGGTTAGCCACGTCATCATCCACAACGTTCACATTCACCACTGTAAACCCTCCGGCAACACTTTGGTCGCTTCGTCGCCTACACATGTTGGATTTCGAGGAGTCTCCGACGGAGACGGAATCTCAGTATCAGCTTCTCATCACATTTGGGTCGATCACTGTTCTCTCGGTTACTGTGCGGACGGACTCATCGACGTCATCCTCGCTTCCACCGCCGTCACAATCTCCAACAACTATTTCTCTCATCACGACGAGGTTATGCTCCTAGGTCACGACGACCGGTACACGGCTGATAAGGGGATGCAAGTAACAATTGCATTCAACCATTTCGGAGAAGGGCTTGTTCAGAGGATGCCGCGGTGTAGGCACGGTTATATCCACGTGGTGAACAACGACTTCACGGCATGGGAAATGTATGCAATCGGCGGAAGTGCTAGCCCTACAATTAATAGTCAGGGTAACCGTTACACCGCACCTATTGATCCCAATGCCAAAGAGGTAACGAAGCGCGTGGACTCAAACGAGAAACATTGGTCGGGATGGAACTGGAGAACGGAAGGTGACGTTATGGTTAACGGAGCTTTTTTCGTGCCGTCAGGTGACGGAGTGAGCCCAGCGTATGCCAGAGCCACCAGTGTTCAGCCTAAAGCCGCCGCGATCATTGACCAGCTCACGGTCAATGCCGGCGTTTTCGGCGATCCAAGTGGGAGAAACGGCCAAGGAGGAAGCTTTCCCGGAATTACGAACGGTGGTGGGACCATCACGCGCGGTTACAGTAAAAGTGGACCTGCCGGCGGTGGCAGCGGCAGTGACAGTGATGATGGGCTTTTCACATTGATATTCGGTAACAATAGCGGTGCGGTGGCTCTAAGGCCGGGACAGGTCTGGTCGATTCTATTGATTATCATTTTATATTGGTATATTCCACACCATACGAGATCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002376 GO:0003674 GO:0003824 GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0009605 GO:0009607 GO:0009814 GO:0009825 GO:0009987 GO:0016020 GO:0016043 GO:0016049 GO:0016829 GO:0016835 GO:0016837 GO:0030570 GO:0031224 GO:0031225 GO:0031226 GO:0040007 GO:0042545 GO:0042547 GO:0043207 GO:0044425 GO:0044459 GO:0044464 GO:0045087 GO:0045229 GO:0046658 GO:0050896 GO:0051704 GO:0051707 GO:0071554 GO:0071555 GO:0071840 GO:0071944 GO:0098542

KEGG Pathways

Metabolic & Signaling

ko00040 ko02024 map00040 map02024

Pfam Domains

Protein Families

Pec_lyase_C

Protein Analysis

518

Amino Acids

56.04

Weight (kDa)

6.72

Isoelectric Point (pI)

32.72

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pectate_lyase_4	PF00544	174 - 378	2.8e-25	Pectate lyase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006501)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G54920 AT5G04310 AT5G04310 AT5G04310
fragaria_vesca	FvH4_7g18400 FvH4_7g18400 FvH4_7g18400 FvH4_7g18400 FvH4_7g18400
malus_domestica	MD01G1100600.v1.1
prunus_persica	Prupe.2G206100_v2.0.a1 Prupe.2G206100_v2.0.a1
pyrus_communis	pycom01g12750 pycom07g16230
rosa_chinensis	RchiOBHm_Chr1g0362211
rosa_laevigata	RLG00000027666
rosa_multiflora	Rmu_co8314571.1_g000001 Rmu_sc0001237.1_g000010
rosa_roxburghii	Rroxscaffold_4G00294430
rosa_rugosa	Rorug01G0295600 Rorug01G0295700 Rorug01G0295800.1
rosa_samantha	Rh1AG305100 Rh1BG267900 Rh1CG286100 Rh1DG297900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	810
Acc36I	ACCTGC	2 cut(s)	604, 1408
AccB1I	GGYRCC	1 cut(s)	242
AccII	CGCG	5 cut(s)	970, 1122, 1268, 1378, 1380
AclI	AACGTT	1 cut(s)	639
AclWI	GGATC	5 cut(s)	16, 29, 497, 1088, 1304
AcoI	YGGCCR	2 cut(s)	589, 1327
AcvI	CACGTG	1 cut(s)	994
AcyI	GRCGYC	2 cut(s)	251, 807
AdeI	CACNNNGTG	2 cut(s)	466, 997
AfaI	GTAC	1 cut(s)	896
AfiI	CCNNNNNNNGG	4 cut(s)	446, 898, 1285, 1406
AflIII	ACRYGT	2 cut(s)	163, 697
AgeI	ACCGGT	1 cut(s)	891
AgsI	TTSAA	4 cut(s)	35, 127, 182, 934
AjiI	CACGTC	1 cut(s)	625
AjuI	GAANNNNNNNTTGG	2 cut(s)	833, 865
AluBI	AGCT	6 cut(s)	532, 546, 746, 1193, 1282, 1341
AluI	AGCT	6 cut(s)	532, 546, 746, 1193, 1282, 1341
Alw21I	GWGCWC	1 cut(s)	534
Alw26I	GTCTC	2 cut(s)	720, 721
AlwI	GGATC	5 cut(s)	16, 29, 497, 1088, 1304
AoxI	GGCC	3 cut(s)	589, 1327, 1484
ApeKI	GCWGC	3 cut(s)	306, 431, 1413
AseI	ATTAAT	1 cut(s)	1059
AsiGI	ACCGGT	1 cut(s)	891
AspA2I	CCTAGG	1 cut(s)	877
AspLEI	GCGC	3 cut(s)	306, 1122, 1380
AspS9I	GGNCC	2 cut(s)	1368, 1397
AsuC2I	CCSGG	3 cut(s)	447, 1347, 1488
AsuHPI	GGTGA	9 cut(s)	80, 249, 268, 400, 577, 641, 1009, 1184, 1223
AsuNHI	GCTAGC	1 cut(s)	1046
AvaII	GGWCC	2 cut(s)	1368, 1397
AvrII	CCTAGG	1 cut(s)	877
BamHI	GGATCC	1 cut(s)	21
BanI	GGYRCC	1 cut(s)	242
BanII	GRGCYC	2 cut(s)	534, 1226
BauI	CACGAG	1 cut(s)	527
BbrPI	CACGTG	1 cut(s)	994
BbsI	GAAGAC	1 cut(s)	217
Bbv12I	GWGCWC	1 cut(s)	534
BbvCI	CCTCAGC	1 cut(s)	112
BbvI	GCAGC	3 cut(s)	293, 443, 1425
BccI	CCATC	4 cut(s)	554, 1146, 1379, 1427
BceAI	ACGGC	8 cut(s)	256, 264, 449, 812, 915, 1029, 1189, 1342
BcnI	CCSGG	3 cut(s)	447, 1347, 1488
BcoDI	GTCTC	2 cut(s)	720, 721
BfaI	CTAG	2 cut(s)	878, 1047
BfoI	RGCGCY	1 cut(s)	307
BfuAI	ACCTGC	2 cut(s)	604, 1408
BisI	GCNGC	7 cut(s)	307, 432, 968, 1263, 1266, 1414, 1417
BlnI	CCTAGG	1 cut(s)	877
BlsI	GCNGC	7 cut(s)	308, 433, 969, 1264, 1267, 1415, 1418
Bme1390I	CCNGG	3 cut(s)	447, 1347, 1488
Bme18I	GGWCC	2 cut(s)	1368, 1397
BmgBI	CACGTC	1 cut(s)	625
BmgT120I	GGNCC	2 cut(s)	1368, 1397
BmiI	GGNNCC	3 cut(s)	23, 244, 1369
BmrFI	CCNGG	3 cut(s)	447, 1347, 1488
BmsI	GCATC	5 cut(s)	110, 125, 282, 903, 954
BmtI	GCTAGC	1 cut(s)	1050
BpiI	GAAGAC	1 cut(s)	217
BpmI	CTGGAG	2 cut(s)	484, 1180
Bpu10I	CCTNAGC	1 cut(s)	112
BpuEI	CTTGAG	1 cut(s)	190
BpuMI	CCSGG	3 cut(s)	447, 1347, 1488
Bsa29I	ATCGAT	1 cut(s)	558
BsaAI	YACGTR	1 cut(s)	994
BsaHI	GRCGYC	2 cut(s)	251, 807
BsaJI	CCNNGG	6 cut(s)	91, 246, 592, 877, 968, 1330
BsaWI	WCCGGW	2 cut(s)	278, 891
Bsc4I	CCNNNNNNNGG	4 cut(s)	446, 898, 1285, 1406
Bse118I	RCCGGY	4 cut(s)	587, 891, 1295, 1403
Bse1I	ACTGG	3 cut(s)	323, 1163, 1245
BseCI	ATCGAT	1 cut(s)	558
BseDI	CCNNGG	6 cut(s)	91, 246, 592, 877, 968, 1330
BseGI	GGATG	5 cut(s)	630, 810, 918, 969, 1157
BseLI	CCNNNNNNNGG	4 cut(s)	446, 898, 1285, 1406
BseMII	CTCAG	2 cut(s)	126, 750
BseNI	ACTGG	3 cut(s)	323, 1163, 1245
BseRI	GAGGAG	2 cut(s)	31, 726
BseXI	GCAGC	3 cut(s)	293, 443, 1425
BseYI	CCCAGC	1 cut(s)	1225
Bsh1236I	CGCG	5 cut(s)	970, 1122, 1268, 1378, 1380
Bsh1285I	CGRYCG	1 cut(s)	892
BshFI	GGCC	3 cut(s)	591, 1329, 1486
BshNI	GGYRCC	1 cut(s)	242
BshTI	ACCGGT	1 cut(s)	891
BshVI	ATCGAT	1 cut(s)	558
BsiEI	CGRYCG	1 cut(s)	892
BsiHKAI	GWGCWC	1 cut(s)	534
BsiSI	CCGG	9 cut(s)	279, 447, 588, 666, 892, 1296, 1347, 1404, 1487
BslFI	GGGAC	2 cut(s)	1381, 1503
BslI	CCNNNNNNNGG	4 cut(s)	446, 898, 1285, 1406
BsmAI	GTCTC	2 cut(s)	720, 721
BsmBI	CGTCTC	1 cut(s)	720
BsmFI	GGGAC	2 cut(s)	1381, 1503
BsmI	GAATGC	1 cut(s)	929
BsnI	GGCC	3 cut(s)	591, 1329, 1486
Bsp1286I	GDGCHC	2 cut(s)	534, 1226
Bsp143I	GATC	7 cut(s)	21, 489, 766, 1093, 1269, 1309, 1550
Bsp19I	CCATGG	2 cut(s)	91, 592
BspANI	GGCC	3 cut(s)	591, 1329, 1486
BspCNI	CTCAG	2 cut(s)	125, 749
BspDI	ATCGAT	1 cut(s)	558
BspFNI	CGCG	5 cut(s)	970, 1122, 1268, 1378, 1380
BspHI	TCATGA	1 cut(s)	10
BspLI	GGNNCC	3 cut(s)	23, 244, 1369
BspMI	ACCTGC	2 cut(s)	604, 1408
BspOI	GCTAGC	1 cut(s)	1050
BspPI	GGATC	5 cut(s)	16, 29, 497, 1088, 1304
BspQI	GCTCTTC	1 cut(s)	91
BspT107I	GGYRCC	1 cut(s)	242
BsrFI	RCCGGY	4 cut(s)	587, 891, 1295, 1403
BsrI	ACTGG	3 cut(s)	323, 1163, 1245
BssAI	RCCGGY	4 cut(s)	587, 891, 1295, 1403
BssECI	CCNNGG	6 cut(s)	91, 246, 592, 877, 968, 1330
BssMI	GATC	7 cut(s)	21, 489, 766, 1093, 1269, 1309, 1550
BssNI	GRCGYC	2 cut(s)	251, 807
BssSI	CACGAG	1 cut(s)	527
BssT1I	CCWWGG	4 cut(s)	91, 592, 877, 1330
Bst2BI	CACGAG	1 cut(s)	527
Bst4CI	ACNGT	9 cut(s)	656, 773, 788, 983, 1076, 1288, 1361, 1388, 1427
Bst6I	CTCTTC	3 cut(s)	91, 459, 559
BstACI	GRCGYC	2 cut(s)	251, 807
BstBAI	YACGTR	1 cut(s)	994
BstC8I	GCNNGC	3 cut(s)	1048, 1297, 1405
BstDEI	CTNAG	3 cut(s)	112, 736, 1481
BstDSI	CCRYGG	4 cut(s)	91, 246, 592, 968
BstEII	GGTNACC	1 cut(s)	1070
BstF5I	GGATG	5 cut(s)	630, 810, 918, 969, 1157
BstFNI	CGCG	5 cut(s)	970, 1122, 1268, 1378, 1380
BstH2I	RGCGCY	1 cut(s)	307
BstHHI	GCGC	3 cut(s)	306, 1122, 1380
BstKTI	GATC	7 cut(s)	24, 492, 769, 1096, 1272, 1312, 1553
BstMAI	GTCTC	2 cut(s)	720, 721
BstMBI	GATC	7 cut(s)	21, 489, 766, 1093, 1269, 1309, 1550
BstMCI	CGRYCG	1 cut(s)	892
BstMWI	GCNNNNNNNGC	5 cut(s)	597, 824, 976, 1305, 1413
BstNSI	RCATGY	2 cut(s)	167, 701
BstPI	GGTNACC	1 cut(s)	1070
BstSCI	CCNGG	3 cut(s)	445, 1345, 1486
BstUI	CGCG	5 cut(s)	970, 1122, 1268, 1378, 1380
BstV1I	GCAGC	3 cut(s)	293, 443, 1425
BstV2I	GAAGAC	1 cut(s)	217
BstX2I	RGATCY	1 cut(s)	21
BstXI	CCANNNNNNTGG	1 cut(s)	508
BstYI	RGATCY	1 cut(s)	21
Bsu15I	ATCGAT	1 cut(s)	558
BsuRI	GGCC	3 cut(s)	591, 1329, 1486
BsuTUI	ATCGAT	1 cut(s)	558
BtgI	CCRYGG	4 cut(s)	91, 246, 592, 968
BtrI	CACGTC	1 cut(s)	625
BtsCI	GGATG	5 cut(s)	630, 810, 918, 969, 1157
BtsI	GCAGTG	1 cut(s)	1426
BtsIMutI	CAGTG	5 cut(s)	652, 769, 1252, 1426, 1432
BveI	ACCTGC	2 cut(s)	604, 1408
Cac8I	GCNNGC	3 cut(s)	1048, 1297, 1405
CciI	TCATGA	1 cut(s)	10
CfoI	GCGC	3 cut(s)	306, 1122, 1380
Cfr10I	RCCGGY	4 cut(s)	587, 891, 1295, 1403
Cfr13I	GGNCC	2 cut(s)	1368, 1397
Cfr42I	CCGCGG	1 cut(s)	971
ClaI	ATCGAT	1 cut(s)	558
CseI	GACGC	2 cut(s)	240, 437
Csp6I	GTAC	1 cut(s)	895
CspAI	ACCGGT	1 cut(s)	891
CviAII	CATG	7 cut(s)	11, 92, 164, 508, 593, 698, 1017
CviQI	GTAC	1 cut(s)	895
DdeI	CTNAG	3 cut(s)	112, 736, 1481
DpnI	GATC	7 cut(s)	23, 491, 768, 1095, 1271, 1311, 1552
DpnII	GATC	7 cut(s)	21, 489, 766, 1093, 1269, 1309, 1550
DraIII	CACNNNGTG	2 cut(s)	466, 997
EaeI	YGGCCR	2 cut(s)	589, 1327
Eam1104I	CTCTTC	3 cut(s)	91, 459, 559
EarI	CTCTTC	3 cut(s)	91, 459, 559
EciI	GGCGGA	1 cut(s)	1053
Ecl136II	GAGCTC	1 cut(s)	532
Eco130I	CCWWGG	4 cut(s)	91, 592, 877, 1330
Eco24I	GRGCYC	2 cut(s)	534, 1226
Eco47I	GGWCC	2 cut(s)	1368, 1397
Eco53kI	GAGCTC	1 cut(s)	532
Eco72I	CACGTG	1 cut(s)	994
Eco91I	GGTNACC	1 cut(s)	1070
EcoICRI	GAGCTC	1 cut(s)	532
EcoO65I	GGTNACC	1 cut(s)	1070
EcoT14I	CCWWGG	4 cut(s)	91, 592, 877, 1330
EcoT38I	GRGCYC	2 cut(s)	534, 1226
ErhI	CCWWGG	4 cut(s)	91, 592, 877, 1330
Esp3I	CGTCTC	1 cut(s)	720
FaeI	CATG	7 cut(s)	14, 95, 167, 511, 596, 701, 1020
FaqI	GGGAC	2 cut(s)	1381, 1503
FatI	CATG	7 cut(s)	10, 91, 163, 507, 592, 697, 1016
Fnu4HI	GCNGC	7 cut(s)	307, 432, 968, 1263, 1266, 1414, 1417
FokI	GGATG	5 cut(s)	617, 797, 925, 976, 1164
FriOI	GRGCYC	2 cut(s)	534, 1226
Fsp4HI	GCNGC	7 cut(s)	307, 432, 968, 1263, 1266, 1414, 1417
FspBI	CTAG	2 cut(s)	878, 1047
GlaI	GCGC	3 cut(s)	305, 1121, 1379
GluI	GCNGC	7 cut(s)	307, 432, 968, 1263, 1266, 1414, 1417
GsaI	CCCAGC	1 cut(s)	1229
GsuI	CTGGAG	2 cut(s)	484, 1180
HaeII	RGCGCY	1 cut(s)	307
HaeIII	GGCC	3 cut(s)	591, 1329, 1486
HapII	CCGG	9 cut(s)	279, 447, 588, 666, 892, 1296, 1347, 1404, 1487
HgaI	GACGC	2 cut(s)	240, 437
HhaI	GCGC	3 cut(s)	306, 1122, 1380
Hin1I	GRCGYC	2 cut(s)	251, 807
Hin1II	CATG	7 cut(s)	14, 95, 167, 511, 596, 701, 1020
Hin6I	GCGC	3 cut(s)	304, 1120, 1378
HinP1I	GCGC	3 cut(s)	304, 1120, 1378
HincII	GTYRAC	1 cut(s)	1186
HindII	GTYRAC	1 cut(s)	1186
HindIII	AAGCTT	1 cut(s)	1339
HpaI	GTTAAC	1 cut(s)	1186
HpaII	CCGG	9 cut(s)	279, 447, 588, 666, 892, 1296, 1347, 1404, 1487
HphI	GGTGA	9 cut(s)	80, 249, 268, 400, 577, 641, 1009, 1184, 1223
Hpy166II	GTNNAC	7 cut(s)	643, 659, 897, 1000, 1126, 1186, 1397
Hpy188I	TCNGA	7 cut(s)	365, 380, 421, 542, 721, 944, 960
Hpy188III	TCNNGA	6 cut(s)	11, 207, 604, 860, 884, 1149
Hpy8I	GTNNAC	7 cut(s)	643, 659, 897, 1000, 1126, 1186, 1397
Hpy99I	CGWCG	9 cut(s)	242, 256, 296, 431, 691, 725, 809, 866, 890
HpyAV	CCTTC	3 cut(s)	276, 941, 1163
HpyCH4III	ACNGT	9 cut(s)	656, 773, 788, 983, 1076, 1288, 1361, 1388, 1427
HpyCH4IV	ACGT	6 cut(s)	44, 624, 639, 807, 993, 1176
HpyCH4V	TGCA	6 cut(s)	48, 54, 273, 916, 929, 1031
HpyF10VI	GCNNNNNNNGC	5 cut(s)	597, 824, 976, 1305, 1413
HpyF3I	CTNAG	3 cut(s)	112, 736, 1481
HpySE526I	ACGT	6 cut(s)	44, 624, 639, 807, 993, 1176
Hsp92I	GRCGYC	2 cut(s)	251, 807
Hsp92II	CATG	7 cut(s)	14, 95, 167, 511, 596, 701, 1020
HspAI	GCGC	3 cut(s)	304, 1120, 1378
KroI	GCCGGC	2 cut(s)	1295, 1403
KroNI	GCCGGC	2 cut(s)	1297, 1405
KspAI	GTTAAC	1 cut(s)	1186
KspI	CCGCGG	1 cut(s)	971
Kzo9I	GATC	7 cut(s)	21, 489, 766, 1093, 1269, 1309, 1550
LguI	GCTCTTC	1 cut(s)	91
LmnI	GCTCC	2 cut(s)	879, 1190
Lsp1109I	GCAGC	3 cut(s)	293, 443, 1425
LweI	GCATC	5 cut(s)	110, 125, 282, 903, 954
MaeI	CTAG	2 cut(s)	878, 1047
MaeII	ACGT	6 cut(s)	44, 624, 639, 807, 993, 1176
MalI	GATC	7 cut(s)	23, 491, 768, 1095, 1271, 1311, 1552
MboI	GATC	7 cut(s)	21, 489, 766, 1093, 1269, 1309, 1550
MboII	GAAGA	7 cut(s)	23, 108, 115, 222, 446, 487, 576
MfeI	CAATTG	2 cut(s)	354, 924
MflI	RGATCY	1 cut(s)	21
MhlI	GDGCHC	2 cut(s)	534, 1226
MluCI	AATT	5 cut(s)	354, 441, 924, 1056, 1350
MlyI	GAGTC	4 cut(s)	333, 723, 792, 1121
MmeI	TCCRAC	4 cut(s)	444, 682, 744, 864
MroNI	GCCGGC	2 cut(s)	1295, 1403
MseI	TTAA	3 cut(s)	174, 1059, 1185
MspA1I	CMGCKG	2 cut(s)	970, 1416
MspI	CCGG	9 cut(s)	279, 447, 588, 666, 892, 1296, 1347, 1404, 1487
MspR9I	CCNGG	3 cut(s)	447, 1347, 1488
MunI	CAATTG	2 cut(s)	354, 924
Mva1269I	GAATGC	1 cut(s)	929
MvnI	CGCG	5 cut(s)	970, 1122, 1268, 1378, 1380
MwoI	GCNNNNNNNGC	5 cut(s)	597, 824, 976, 1305, 1413
NaeI	GCCGGC	2 cut(s)	1297, 1405
NciI	CCSGG	3 cut(s)	447, 1347, 1488
NcoI	CCATGG	2 cut(s)	91, 592
NdeII	GATC	7 cut(s)	21, 489, 766, 1093, 1269, 1309, 1550
NgoMIV	GCCGGC	2 cut(s)	1295, 1403
NheI	GCTAGC	1 cut(s)	1046
NlaIII	CATG	7 cut(s)	14, 95, 167, 511, 596, 701, 1020
NlaIV	GGNNCC	3 cut(s)	23, 244, 1369
NmuCI	GTSAC	7 cut(s)	255, 406, 829, 881, 1172, 1211, 1421
NspI	RCATGY	2 cut(s)	167, 701
PagI	TCATGA	1 cut(s)	10
PciI	ACATGT	2 cut(s)	163, 697
PciSI	GCTCTTC	1 cut(s)	91
PcsI	WCGNNNNNNNCGW	1 cut(s)	801
PctI	GAATGC	1 cut(s)	929
PdiI	GCCGGC	2 cut(s)	1297, 1405
PfeI	GAWTC	8 cut(s)	197, 413, 436, 469, 513, 607, 732, 1504
PfoI	TCCNGGA	2 cut(s)	445, 1345
PinAI	ACCGGT	1 cut(s)	891
PkrI	GCNGC	7 cut(s)	308, 433, 969, 1264, 1267, 1415, 1418
PleI	GAGTC	4 cut(s)	333, 722, 792, 1121
PmaCI	CACGTG	1 cut(s)	994
PmlI	CACGTG	1 cut(s)	994
PpsI	GAGTC	4 cut(s)	333, 722, 792, 1121
Ppu21I	YACGTR	1 cut(s)	994
PscI	ACATGT	2 cut(s)	163, 697
PshBI	ATTAAT	1 cut(s)	1059
Psp124BI	GAGCTC	1 cut(s)	534
Psp1406I	AACGTT	1 cut(s)	639
PspCI	CACGTG	1 cut(s)	994
PspEI	GGTNACC	1 cut(s)	1070
PspFI	CCCAGC	1 cut(s)	1225
PspN4I	GGNNCC	3 cut(s)	23, 244, 1369
PspPI	GGNCC	2 cut(s)	1368, 1397
PsuI	RGATCY	1 cut(s)	21
RsaI	GTAC	1 cut(s)	896
RsaNI	GTAC	1 cut(s)	895
SacI	GAGCTC	1 cut(s)	534
SacII	CCGCGG	1 cut(s)	971
SapI	GCTCTTC	1 cut(s)	91
SaqAI	TTAA	3 cut(s)	174, 1059, 1185
SatI	GCNGC	7 cut(s)	307, 432, 968, 1263, 1266, 1414, 1417
Sau3AI	GATC	7 cut(s)	21, 489, 766, 1093, 1269, 1309, 1550
Sau96I	GGNCC	2 cut(s)	1368, 1397
SchI	GAGTC	4 cut(s)	333, 723, 792, 1121
ScrFI	CCNGG	3 cut(s)	447, 1347, 1488
SduI	GDGCHC	2 cut(s)	534, 1226
SfaNI	GCATC	5 cut(s)	110, 125, 282, 903, 954
Sfr303I	CCGCGG	1 cut(s)	971
SgrBI	CCGCGG	1 cut(s)	971
SinI	GGWCC	2 cut(s)	1368, 1397
SmlI	CTYRAG	1 cut(s)	205
SmoI	CTYRAG	1 cut(s)	205
Sse9I	AATT	5 cut(s)	354, 441, 924, 1056, 1350
SspMI	CTAG	2 cut(s)	878, 1047
SstI	GAGCTC	1 cut(s)	534
StyD4I	CCNGG	3 cut(s)	445, 1345, 1486
StyI	CCWWGG	4 cut(s)	91, 592, 877, 1330
TaaI	ACNGT	9 cut(s)	656, 773, 788, 983, 1076, 1288, 1361, 1388, 1427
TaiI	ACGT	6 cut(s)	47, 627, 642, 810, 996, 1179
TaqI	TCGA	7 cut(s)	291, 457, 558, 709, 765, 804, 1502
TasI	AATT	5 cut(s)	354, 441, 924, 1056, 1350
TauI	GCSGC	4 cut(s)	970, 1265, 1268, 1419
TfiI	GAWTC	8 cut(s)	197, 413, 436, 469, 513, 607, 732, 1504
Tru1I	TTAA	3 cut(s)	174, 1059, 1185
Tru9I	TTAA	3 cut(s)	174, 1059, 1185
TscAI	CASTG	5 cut(s)	659, 776, 1252, 1426, 1432
TseFI	GTSAC	7 cut(s)	255, 406, 829, 881, 1172, 1211, 1421
TseI	GCWGC	3 cut(s)	306, 431, 1413
Tsp45I	GTSAC	7 cut(s)	255, 406, 829, 881, 1172, 1211, 1421
TspGWI	ACGGA	6 cut(s)	738, 744, 810, 1181, 1203, 1230
TspRI	CASTG	5 cut(s)	659, 776, 1252, 1426, 1432
VpaK11BI	GGWCC	2 cut(s)	1368, 1397
VspI	ATTAAT	1 cut(s)	1059
XceI	RCATGY	2 cut(s)	167, 701
XcmI	CCANNNNNNNNNTGG	1 cut(s)	483
XmaJI	CCTAGG	1 cut(s)	877
XspI	CTAG	2 cut(s)	878, 1047
ZraI	GACGTC	1 cut(s)	808

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.