Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr6

Orthogroup ID

OG0006501

Physical Location & Seq

Genomic Coordinates Reverse (-)

13712526 .. 13717054

Gene Structure 4529 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000027666

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1491 bp

ATGCTTCCTACCACCTGCATTCTCTTAATATGCCTCTTGAGCTCTCTCTGTTCCCTCACCTTAGCTTCTCTAAACCTCAACCTCACCCTCCCTCATCAACACCCTTACCCTGAAGCTGTGGCTCAAGAAGTACAAAGGAGAGTCAATGCCTCCCTTTCCCGGCGCGAAATGCTGTCCCTCAAGGACCAGCAATGCGCCGTCGGCAACCCCATCGACGACTGCTGGCGCTGCGACTCCAACTGGGCCAACGACCGCCAGAAGCTAGCCGACTGCGGCATCGGCTTCGGCCAAGACGCCATGGGAGGTAAGGGCGGCCAGATCTACATCGTCACCGACTCCTCCGACCGCGACCCCGCCAACCCAACTCCGGGCACTCTCCGCCACGCCGTGATCCAAACCGAACCCCTCTGGATCATCTTCTCCGCCGACATGACCATCAAGCTCAAAGTCGAGCTCATCGTCAACAGCTTCAAGACCATCGACGGCCGCGGCGTTAGTGTCCACGTCACCGGCGGCGGATGCATAACCCTCCAGTACGTCTCCAACGTCATCATCCACAACATTCACGTCCACCACTGCAAGCCCGCCGGCAACACCAACATCGCCTCCAGCCCCACCCACGTCGGCTGGCGCGGGAAGTCCGACGGCGACGGCATCTCCCTCTTCAGCGCCCGCAAAATTTGGATAGACCACTGCTCCCTGTCGTACTGCGCCGACGGACTCATCGACGCCATAATGGGGTCCACCGGGATCACGATTTCCAACAACCACTTTGCGCACCACGACGAGGTGATGCTTCTCGGCCACAACGATAAGTACTCGCCGGACACCGGGATGCAGGTGACGATTGCGTTCAACCACTTCGGGGAGGCGCTGGTGCAGCGTATGCCTCGGTGCAGACTCGGGTATATTCACGTGGTGAACAATGACTTCACGCAGTGGGAGATGTACGCTATCGGCGGCAGCGCCAACCCCACCATTAACAGCCAGGGGAATCGGTACACTGCTCCGACGGACCAGAACGCCAAGGAGGTGACAAAGCGCGTGGACACGAACGAAGGGGACTGGGCGGACTGGAACTGGAGAACGGAGGGGGACATAATGGTAAATGGAGCCTTCTTCGTGCCATCAGGTGCGGGCATGAGCACCCAGTACGCTAAGGCCTCAAGCCTCGAGCCCAAGTCGGTGGCTCTCATTGACCGCCTCACCGTCACCGCCGGTGTATTCGGCGACCCAAGGGACAGTAGTAGGAGCGAATCATACCCCGGATACAGCGGCGGTGGGACTGTAACCGATGGTACCACCAACACCGGCAGTGGAGGGTCCAGCGGCGGTGACGACTATTTTGGAATGATATTCGGGAACAGTGCTCAACCATCATCATCTTCAAACGCATTCTTGTTGTCTCTTCTAATTATCTTAATTTTGTATGTTACCACCAGCCATGGTGGTGTATTATCATTACCATTATTCTTAACATTATTATTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002376 GO:0003674 GO:0003824 GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0009605 GO:0009607 GO:0009814 GO:0009825 GO:0009987 GO:0016020 GO:0016043 GO:0016049 GO:0016829 GO:0016835 GO:0016837 GO:0030570 GO:0031224 GO:0031225 GO:0031226 GO:0040007 GO:0042545 GO:0042547 GO:0043207 GO:0044425 GO:0044459 GO:0044464 GO:0045087 GO:0045229 GO:0046658 GO:0050896 GO:0051704 GO:0051707 GO:0071554 GO:0071555 GO:0071840 GO:0071944 GO:0098542

KEGG Pathways

Metabolic & Signaling

ko00040 ko02024 map00040 map02024

Pfam Domains

Protein Families

Pec_lyase_C

Protein Analysis

497

Amino Acids

53.52

Weight (kDa)

5.82

Isoelectric Point (pI)

28.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pectate_lyase_4	PF00544	151 - 351	1.7e-21	Pectate lyase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006501)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G54920 AT5G04310 AT5G04310 AT5G04310
fragaria_vesca	FvH4_7g18400 FvH4_7g18400 FvH4_7g18400 FvH4_7g18400 FvH4_7g18400
malus_domestica	MD01G1100600.v1.1
prunus_persica	Prupe.2G206100_v2.0.a1 Prupe.2G206100_v2.0.a1
pyrus_communis	pycom01g12750 pycom07g16230
rosa_chinensis	RchiOBHm_Chr1g0362211
rosa_laevigata	RLG00000027666
rosa_multiflora	Rmu_co8314571.1_g000001 Rmu_sc0001237.1_g000010
rosa_roxburghii	Rroxscaffold_4G00294430
rosa_rugosa	Rorug01G0295600 Rorug01G0295700 Rorug01G0295800.1
rosa_samantha	Rh1AG305100 Rh1BG267900 Rh1CG286100 Rh1DG297900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1489
AarI	CACCTGC	2 cut(s)	23, 829
Acc16I	TGCGCA	1 cut(s)	777
Acc36I	ACCTGC	2 cut(s)	23, 829
Acc65I	GGTACC	1 cut(s)	1298
AccB1I	GGYRCC	1 cut(s)	1298
AccII	CGCG	5 cut(s)	165, 348, 489, 633, 1044
AclWI	GGATC	3 cut(s)	385, 419, 758
AcoI	YGGCCR	4 cut(s)	286, 313, 484, 802
AcsI	RAATTY	1 cut(s)	678
AcuI	CTGAAG	2 cut(s)	132, 649
AcvI	CACGTG	1 cut(s)	916
AcyI	GRCGYC	2 cut(s)	294, 729
AdeI	CACNNNGTG	3 cut(s)	388, 919, 939
AfaI	GTAC	8 cut(s)	132, 536, 707, 818, 950, 1001, 1154, 1300
AfiI	CCNNNNNNNGG	7 cut(s)	159, 367, 368, 738, 787, 830, 865
AgsI	TTSAA	3 cut(s)	472, 856, 1389
AjiI	CACGTC	3 cut(s)	505, 568, 622
AjnI	CCWGG	1 cut(s)	987
AluBI	AGCT	7 cut(s)	42, 65, 116, 262, 442, 454, 468
AluI	AGCT	7 cut(s)	42, 65, 116, 262, 442, 454, 468
Alw21I	GWGCWC	4 cut(s)	44, 456, 1148, 1372
Alw26I	GTCTC	2 cut(s)	544, 1410
AlwI	GGATC	3 cut(s)	385, 419, 758
Ama87I	CYCGRG	2 cut(s)	902, 1172
AoxI	GGCC	6 cut(s)	243, 286, 313, 484, 802, 1161
ApeKI	GCWGC	3 cut(s)	228, 880, 963
ApoI	RAATTY	1 cut(s)	678
ArsI	GACNNNNNNTTYG	2 cut(s)	1328, 1360
Asp718I	GGTACC	1 cut(s)	1298
AspS9I	GGNCC	5 cut(s)	184, 243, 741, 1015, 1323
AsuC2I	CCSGG	5 cut(s)	160, 369, 748, 832, 1266
AsuNHI	GCTAGC	1 cut(s)	262
AvaI	CYCGRG	2 cut(s)	902, 1172
AvaII	GGWCC	4 cut(s)	184, 741, 1015, 1323
BaeGI	GKGCMC	1 cut(s)	374
BanI	GGYRCC	1 cut(s)	1298
BanII	GRGCYC	3 cut(s)	44, 456, 1179
BbrPI	CACGTG	1 cut(s)	916
Bbv12I	GWGCWC	4 cut(s)	44, 456, 1148, 1372
BbvI	GCAGC	3 cut(s)	215, 892, 975
BccI	CCATC	6 cut(s)	218, 443, 485, 1135, 1289, 1384
BceAI	ACGGC	5 cut(s)	182, 371, 499, 661, 667
BcgI	CGANNNNNNTGC	4 cut(s)	193, 227, 265, 299
BciT130I	CCWGG	1 cut(s)	989
BciVI	GTATCC	1 cut(s)	1262
BcnI	CCSGG	5 cut(s)	160, 369, 748, 832, 1266
BcoDI	GTCTC	2 cut(s)	544, 1410
BfaI	CTAG	1 cut(s)	263
BfoI	RGCGCY	4 cut(s)	229, 672, 875, 969
BfuAI	ACCTGC	2 cut(s)	23, 829
BfuI	GTATCC	1 cut(s)	1262
BglII	AGATCT	1 cut(s)	318
BmcAI	AGTACT	1 cut(s)	818
Bme1390I	CCNGG	6 cut(s)	160, 369, 748, 832, 989, 1266
Bme18I	GGWCC	4 cut(s)	184, 741, 1015, 1323
BmeT110I	CYCGRG	2 cut(s)	902, 1172
BmgBI	CACGTC	3 cut(s)	505, 568, 622
BmgT120I	GGNCC	5 cut(s)	184, 243, 741, 1015, 1323
BmiI	GGNNCC	4 cut(s)	742, 1114, 1300, 1324
BmrFI	CCNGG	6 cut(s)	160, 369, 748, 832, 989, 1266
BmrI	ACTGGG	3 cut(s)	250, 1075, 1144
BmsI	GCATC	5 cut(s)	285, 509, 663, 783, 825
BmtI	GCTAGC	1 cut(s)	266
BmuI	ACTGGG	3 cut(s)	250, 1075, 1144
BpmI	CTGGAG	3 cut(s)	515, 592, 1102
Bpu10I	CCTNAGC	2 cut(s)	61, 1158
BpuEI	CTTGAG	4 cut(s)	58, 108, 164, 1150
BpuMI	CCSGG	5 cut(s)	160, 369, 748, 832, 1266
BsaAI	YACGTR	1 cut(s)	916
BsaBI	GATNNNNATC	1 cut(s)	323
BsaHI	GRCGYC	2 cut(s)	294, 729
BsaJI	CCNNGG	8 cut(s)	297, 487, 890, 988, 1026, 1235, 1264, 1444
BsaXI	ACNNNNNCTCC	6 cut(s)	590, 620, 641, 671, 680, 710
Bsc4I	CCNNNNNNNGG	7 cut(s)	159, 367, 368, 738, 787, 830, 865
Bse118I	RCCGGY	4 cut(s)	509, 587, 1217, 1310
Bse1I	ACTGG	6 cut(s)	245, 532, 1070, 1079, 1085, 1150
Bse3DI	GCAATG	1 cut(s)	197
Bse8I	GATNNNNATC	1 cut(s)	323
BseBI	CCWGG	1 cut(s)	989
BseDI	CCNNGG	8 cut(s)	297, 487, 890, 988, 1026, 1235, 1264, 1444
BseGI	GGATG	3 cut(s)	524, 552, 840
BseJI	GATNNNNATC	1 cut(s)	323
BseLI	CCNNNNNNNGG	7 cut(s)	159, 367, 368, 738, 787, 830, 865
BseMI	GCAATG	1 cut(s)	197
BseNI	ACTGG	6 cut(s)	245, 532, 1070, 1079, 1085, 1150
BseRI	GAGGAG	1 cut(s)	328
BseSI	GKGCMC	1 cut(s)	374
BseX3I	CGGCCG	1 cut(s)	484
BseXI	GCAGC	3 cut(s)	215, 892, 975
BsgI	GTGCAG	2 cut(s)	899, 916
Bsh1236I	CGCG	5 cut(s)	165, 348, 489, 633, 1044
Bsh1285I	CGRYCG	3 cut(s)	253, 346, 487
BshFI	GGCC	6 cut(s)	245, 288, 315, 486, 804, 1163
BshNI	GGYRCC	1 cut(s)	1298
BsiEI	CGRYCG	3 cut(s)	253, 346, 487
BsiHKAI	GWGCWC	4 cut(s)	44, 456, 1148, 1372
BsiHKCI	CYCGRG	2 cut(s)	902, 1172
BslFI	GGGAC	5 cut(s)	160, 1076, 1109, 1253, 1297
BslI	CCNNNNNNNGG	7 cut(s)	159, 367, 368, 738, 787, 830, 865
BsmAI	GTCTC	2 cut(s)	544, 1410
BsmBI	CGTCTC	1 cut(s)	544
BsmFI	GGGAC	5 cut(s)	160, 1076, 1109, 1253, 1297
BsmI	GAATGC	2 cut(s)	18, 1394
BsnI	GGCC	6 cut(s)	245, 288, 315, 486, 804, 1163
BsoBI	CYCGRG	2 cut(s)	902, 1172
Bsp1286I	GDGCHC	6 cut(s)	44, 374, 456, 1148, 1179, 1372
Bsp143I	GATC	4 cut(s)	318, 390, 411, 750
Bsp19I	CCATGG	2 cut(s)	297, 1444
BspANI	GGCC	6 cut(s)	245, 288, 315, 486, 804, 1163
BspFNI	CGCG	5 cut(s)	165, 348, 489, 633, 1044
BspLI	GGNNCC	4 cut(s)	742, 1114, 1300, 1324
BspMI	ACCTGC	2 cut(s)	23, 829
BspOI	GCTAGC	1 cut(s)	266
BspPI	GGATC	3 cut(s)	385, 419, 758
BspT107I	GGYRCC	1 cut(s)	1298
BsrDI	GCAATG	1 cut(s)	197
BsrFI	RCCGGY	4 cut(s)	509, 587, 1217, 1310
BsrI	ACTGG	6 cut(s)	245, 532, 1070, 1079, 1085, 1150
BssAI	RCCGGY	4 cut(s)	509, 587, 1217, 1310
BssECI	CCNNGG	8 cut(s)	297, 487, 890, 988, 1026, 1235, 1264, 1444
BssMI	GATC	4 cut(s)	318, 390, 411, 750
BssNI	GRCGYC	2 cut(s)	294, 729
BssT1I	CCWWGG	4 cut(s)	297, 1026, 1235, 1444
Bst2UI	CCWGG	1 cut(s)	989
Bst4CI	ACNGT	4 cut(s)	1210, 1244, 1288, 1367
Bst6I	CTCTTC	2 cut(s)	668, 1413
BstACI	GRCGYC	2 cut(s)	294, 729
BstAPI	GCANNNNNTGC	1 cut(s)	886
BstBAI	YACGTR	1 cut(s)	916
BstC8I	GCNNGC	8 cut(s)	224, 264, 581, 585, 589, 629, 673, 1138
BstDEI	CTNAG	2 cut(s)	61, 1158
BstDSI	CCRYGG	3 cut(s)	297, 487, 1444
BstF5I	GGATG	3 cut(s)	524, 552, 840
BstFNI	CGCG	5 cut(s)	165, 348, 489, 633, 1044
BstH2I	RGCGCY	4 cut(s)	229, 672, 875, 969
BstKTI	GATC	4 cut(s)	321, 393, 414, 753
BstMAI	GTCTC	2 cut(s)	544, 1410
BstMBI	GATC	4 cut(s)	318, 390, 411, 750
BstMCI	CGRYCG	3 cut(s)	253, 346, 487
BstMWI	GCNNNNNNNGC	9 cut(s)	39, 169, 201, 228, 279, 378, 519, 880, 886
BstNI	CCWGG	1 cut(s)	989
BstSCI	CCNGG	6 cut(s)	158, 367, 746, 830, 987, 1264
BstSLI	GKGCMC	1 cut(s)	374
BstUI	CGCG	5 cut(s)	165, 348, 489, 633, 1044
BstV1I	GCAGC	3 cut(s)	215, 892, 975
BstX2I	RGATCY	1 cut(s)	318
BstXI	CCANNNNNNTGG	1 cut(s)	1186
BstYI	RGATCY	1 cut(s)	318
BstZI	CGGCCG	1 cut(s)	484
BsuI	GTATCC	1 cut(s)	1262
BsuRI	GGCC	6 cut(s)	245, 288, 315, 486, 804, 1163
BtgI	CCRYGG	3 cut(s)	297, 487, 1444
BtgZI	GCGATG	1 cut(s)	586
BtrI	CACGTC	3 cut(s)	505, 568, 622
BtsCI	GGATG	3 cut(s)	524, 552, 840
BtsI	GCAGTG	5 cut(s)	574, 691, 944, 1002, 1321
BtsIMutI	CAGTG	6 cut(s)	574, 691, 944, 1002, 1321, 1372
BveI	ACCTGC	2 cut(s)	23, 829
Cac8I	GCNNGC	8 cut(s)	224, 264, 581, 585, 589, 629, 673, 1138
Cfr10I	RCCGGY	4 cut(s)	509, 587, 1217, 1310
Cfr13I	GGNCC	5 cut(s)	184, 243, 741, 1015, 1323
Cfr42I	CCGCGG	1 cut(s)	490
CseI	GACGC	2 cut(s)	302, 737
Csp6I	GTAC	8 cut(s)	131, 535, 706, 817, 949, 1000, 1153, 1299
CspCI	CAANNNNNGTGG	2 cut(s)	1026, 1061
CviAII	CATG	4 cut(s)	298, 430, 1141, 1445
CviQI	GTAC	8 cut(s)	131, 535, 706, 817, 949, 1000, 1153, 1299
DdeI	CTNAG	2 cut(s)	61, 1158
DpnI	GATC	4 cut(s)	320, 392, 413, 752
DpnII	GATC	4 cut(s)	318, 390, 411, 750
DraIII	CACNNNGTG	3 cut(s)	388, 919, 939
EaeI	YGGCCR	4 cut(s)	286, 313, 484, 802
EagI	CGGCCG	1 cut(s)	484
Eam1104I	CTCTTC	2 cut(s)	668, 1413
EarI	CTCTTC	2 cut(s)	668, 1413
EciI	GGCGGA	4 cut(s)	368, 412, 531, 1085
Ecl136II	GAGCTC	2 cut(s)	42, 454
EclXI	CGGCCG	1 cut(s)	484
Eco130I	CCWWGG	4 cut(s)	297, 1026, 1235, 1444
Eco147I	AGGCCT	1 cut(s)	1163
Eco24I	GRGCYC	3 cut(s)	44, 456, 1179
Eco47I	GGWCC	4 cut(s)	184, 741, 1015, 1323
Eco52I	CGGCCG	1 cut(s)	484
Eco53kI	GAGCTC	2 cut(s)	42, 454
Eco57I	CTGAAG	2 cut(s)	132, 649
Eco72I	CACGTG	1 cut(s)	916
Eco88I	CYCGRG	2 cut(s)	902, 1172
EcoICRI	GAGCTC	2 cut(s)	42, 454
EcoRII	CCWGG	1 cut(s)	987
EcoT14I	CCWWGG	4 cut(s)	297, 1026, 1235, 1444
EcoT22I	ATGCAT	1 cut(s)	524
EcoT38I	GRGCYC	3 cut(s)	44, 456, 1179
ErhI	CCWWGG	4 cut(s)	297, 1026, 1235, 1444
Esp3I	CGTCTC	1 cut(s)	544
FaeI	CATG	4 cut(s)	301, 433, 1144, 1448
FaqI	GGGAC	5 cut(s)	160, 1076, 1109, 1253, 1297
FatI	CATG	4 cut(s)	297, 429, 1140, 1444
FauI	CCCGC	5 cut(s)	361, 592, 626, 680, 1129
FokI	GGATG	3 cut(s)	531, 539, 847
FriOI	GRGCYC	3 cut(s)	44, 456, 1179
FspBI	CTAG	1 cut(s)	263
FspI	TGCGCA	1 cut(s)	777
GsuI	CTGGAG	3 cut(s)	515, 592, 1102
HaeII	RGCGCY	4 cut(s)	229, 672, 875, 969
HaeIII	GGCC	6 cut(s)	245, 288, 315, 486, 804, 1163
HgaI	GACGC	2 cut(s)	302, 737
Hin1I	GRCGYC	2 cut(s)	294, 729
Hin1II	CATG	4 cut(s)	301, 433, 1144, 1448
HincII	GTYRAC	1 cut(s)	463
HindII	GTYRAC	1 cut(s)	463
HinfI	GANTC	7 cut(s)	141, 233, 335, 720, 900, 994, 1256
Hpy166II	GTNNAC	7 cut(s)	463, 502, 571, 744, 922, 1002, 1048
Hpy188I	TCNGA	3 cut(s)	343, 643, 1011
Hpy188III	TCNNGA	6 cut(s)	37, 125, 409, 472, 754, 1360
Hpy8I	GTNNAC	7 cut(s)	463, 502, 571, 744, 922, 1002, 1048
HpyAV	CCTTC	2 cut(s)	1052, 1126
HpyCH4III	ACNGT	4 cut(s)	1210, 1244, 1288, 1367
HpyCH4IV	ACGT	6 cut(s)	504, 537, 546, 567, 621, 915
HpyCH4V	TGCA	6 cut(s)	18, 522, 579, 838, 880, 897
HpyF10VI	GCNNNNNNNGC	9 cut(s)	39, 169, 201, 228, 279, 378, 519, 880, 886
HpyF3I	CTNAG	2 cut(s)	61, 1158
HpySE526I	ACGT	6 cut(s)	504, 537, 546, 567, 621, 915
Hsp92I	GRCGYC	2 cut(s)	294, 729
Hsp92II	CATG	4 cut(s)	301, 433, 1144, 1448
KpnI	GGTACC	1 cut(s)	1302
KroI	GCCGGC	1 cut(s)	587
KroNI	GCCGGC	1 cut(s)	589
KspI	CCGCGG	1 cut(s)	490
Kzo9I	GATC	4 cut(s)	318, 390, 411, 750
LmnI	GCTCC	4 cut(s)	701, 1012, 1112, 1251
Lsp1109I	GCAGC	3 cut(s)	215, 892, 975
LweI	GCATC	5 cut(s)	285, 509, 663, 783, 825
MaeI	CTAG	1 cut(s)	263
MaeII	ACGT	6 cut(s)	504, 537, 546, 567, 621, 915
MaeIII	GTNAC	8 cut(s)	328, 505, 841, 1033, 1210, 1288, 1334, 1432
MalI	GATC	4 cut(s)	320, 392, 413, 752
MboI	GATC	4 cut(s)	318, 390, 411, 750
MboII	GAAGA	5 cut(s)	409, 655, 1111, 1377, 1400
MflI	RGATCY	1 cut(s)	318
MhlI	GDGCHC	6 cut(s)	44, 374, 456, 1148, 1179, 1372
MluCI	AATT	3 cut(s)	678, 1413, 1422
MlyI	GAGTC	5 cut(s)	150, 227, 329, 714, 894
MmeI	TCCRAC	6 cut(s)	261, 366, 567, 666, 786, 1034
Mph1103I	ATGCAT	1 cut(s)	524
MroNI	GCCGGC	1 cut(s)	587
MseI	TTAA	4 cut(s)	26, 981, 1421, 1475
MslI	CAYNNNNRTG	2 cut(s)	833, 1449
MspA1I	CMGCKG	3 cut(s)	489, 1275, 1329
MspR9I	CCNGG	6 cut(s)	160, 369, 748, 832, 989, 1266
Mva1269I	GAATGC	2 cut(s)	18, 1394
MvaI	CCWGG	1 cut(s)	989
MvnI	CGCG	5 cut(s)	165, 348, 489, 633, 1044
MwoI	GCNNNNNNNGC	9 cut(s)	39, 169, 201, 228, 279, 378, 519, 880, 886
NaeI	GCCGGC	1 cut(s)	589
NciI	CCSGG	5 cut(s)	160, 369, 748, 832, 1266
NcoI	CCATGG	2 cut(s)	297, 1444
NdeII	GATC	4 cut(s)	318, 390, 411, 750
NgoMIV	GCCGGC	1 cut(s)	587
NheI	GCTAGC	1 cut(s)	262
NlaIII	CATG	4 cut(s)	301, 433, 1144, 1448
NlaIV	GGNNCC	4 cut(s)	742, 1114, 1300, 1324
NmeAIII	GCCGAG	1 cut(s)	780
NmuCI	GTSAC	6 cut(s)	328, 505, 841, 1033, 1210, 1334
NsbI	TGCGCA	1 cut(s)	777
NsiI	ATGCAT	1 cut(s)	524
PaeR7I	CTCGAG	1 cut(s)	1172
PaqCI	CACCTGC	2 cut(s)	23, 829
PceI	AGGCCT	1 cut(s)	1163
PcsI	WCGNNNNNNNCGW	5 cut(s)	456, 489, 543, 723, 807
PctI	GAATGC	2 cut(s)	18, 1394
PdiI	GCCGGC	1 cut(s)	589
PfeI	GAWTC	2 cut(s)	994, 1256
PleI	GAGTC	5 cut(s)	149, 227, 329, 714, 894
PmaCI	CACGTG	1 cut(s)	916
PmlI	CACGTG	1 cut(s)	916
PpsI	GAGTC	5 cut(s)	149, 227, 329, 714, 894
Ppu21I	YACGTR	1 cut(s)	916
PsiI	TTATAA	1 cut(s)	1489
Psp124BI	GAGCTC	2 cut(s)	44, 456
Psp6I	CCWGG	1 cut(s)	987
PspCI	CACGTG	1 cut(s)	916
PspGI	CCWGG	1 cut(s)	987
PspN4I	GGNNCC	4 cut(s)	742, 1114, 1300, 1324
PspPI	GGNCC	5 cut(s)	184, 243, 741, 1015, 1323
PspXI	VCTCGAGB	1 cut(s)	1172
PsuI	RGATCY	1 cut(s)	318
RsaI	GTAC	8 cut(s)	132, 536, 707, 818, 950, 1001, 1154, 1300
RsaNI	GTAC	8 cut(s)	131, 535, 706, 817, 949, 1000, 1153, 1299
RseI	CAYNNNNRTG	2 cut(s)	833, 1449
SacI	GAGCTC	2 cut(s)	44, 456
SacII	CCGCGG	1 cut(s)	490
SaqAI	TTAA	4 cut(s)	26, 981, 1421, 1475
Sau3AI	GATC	4 cut(s)	318, 390, 411, 750
Sau96I	GGNCC	5 cut(s)	184, 243, 741, 1015, 1323
ScaI	AGTACT	1 cut(s)	818
SchI	GAGTC	5 cut(s)	150, 227, 329, 714, 894
ScrFI	CCNGG	6 cut(s)	160, 369, 748, 832, 989, 1266
SduI	GDGCHC	6 cut(s)	44, 374, 456, 1148, 1179, 1372
SfaNI	GCATC	5 cut(s)	285, 509, 663, 783, 825
Sfr274I	CTCGAG	1 cut(s)	1172
Sfr303I	CCGCGG	1 cut(s)	490
SgrAI	CRCCGGYG	2 cut(s)	509, 1217
SgrBI	CCGCGG	1 cut(s)	490
SinI	GGWCC	4 cut(s)	184, 741, 1015, 1323
SlaI	CTCGAG	1 cut(s)	1172
SmiMI	CAYNNNNRTG	2 cut(s)	833, 1449
SmlI	CTYRAG	5 cut(s)	37, 123, 179, 1165, 1172
SmoI	CTYRAG	5 cut(s)	37, 123, 179, 1165, 1172
Sse9I	AATT	3 cut(s)	678, 1413, 1422
SseBI	AGGCCT	1 cut(s)	1163
SspMI	CTAG	1 cut(s)	263
SstI	GAGCTC	2 cut(s)	44, 456
StuI	AGGCCT	1 cut(s)	1163
StyD4I	CCNGG	6 cut(s)	158, 367, 746, 830, 987, 1264
StyI	CCWWGG	4 cut(s)	297, 1026, 1235, 1444
TaaI	ACNGT	4 cut(s)	1210, 1244, 1288, 1367
TaiI	ACGT	6 cut(s)	507, 540, 549, 570, 624, 918
TaqI	TCGA	5 cut(s)	213, 450, 480, 726, 1173
TasI	AATT	3 cut(s)	678, 1413, 1422
TatI	WGTACW	2 cut(s)	130, 816
TauI	GCSGC	8 cut(s)	276, 315, 489, 492, 516, 963, 1278, 1332
TfiI	GAWTC	2 cut(s)	994, 1256
Tru1I	TTAA	4 cut(s)	26, 981, 1421, 1475
Tru9I	TTAA	4 cut(s)	26, 981, 1421, 1475
TscAI	CASTG	6 cut(s)	581, 698, 944, 1009, 1321, 1372
TseFI	GTSAC	6 cut(s)	328, 505, 841, 1033, 1210, 1334
TseI	GCWGC	3 cut(s)	228, 880, 963
Tsp45I	GTSAC	6 cut(s)	328, 505, 841, 1033, 1210, 1334
TspGWI	ACGGA	3 cut(s)	732, 1028, 1103
TspRI	CASTG	6 cut(s)	581, 698, 944, 1009, 1321, 1372
VpaK11BI	GGWCC	4 cut(s)	184, 741, 1015, 1323
XapI	RAATTY	1 cut(s)	678
XhoI	CTCGAG	1 cut(s)	1172
XspI	CTAG	1 cut(s)	263
ZrmI	AGTACT	1 cut(s)	818
Zsp2I	ATGCAT	1 cut(s)	524

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.