Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0006501

Physical Location & Seq

Genomic Coordinates Forward (+)

21371586 .. 21377400

Gene Structure 5815 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1100600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1497 bp

ATGCTTCTTCCTACCACCTGCATTCTCTTAATTTGTTTCTTTCTCTCTCCTCTCTGTAAAGCTTCTTACTCCTTCAACCTCTCCCTCCCTCACCAGCACCCAGACCCTGATGCAGTTGCTCATGAAGTTCAAAGGAGAGTCAATGCGTCTGTTTCCAGGCGCCAAATGCTGTCCATTGACGTCAAGGACCAAAGCCAGTGCCTTGTCGGCAACCCAATCGACGATTGCTGGCGCTGCGAGGACTGGAGCAACAACCGCCAAAAGCTCGCCGATTGCGGCATCGGGTTCGGCATGGACGCCATGGGAGGTAAGGGGGGCCTGATCTACATAGTCACCGACTCGTCGGATTCCAACCCGGCCGACCCAACTCCGGGCACGCTCCGCCACGCCGTCATTCAAACCGAGCCCCTCTGGATCATCTTCTCTGCTGACATGACCATCAAGCTCAAATGCGAGCTTATCGTCAACAGCTTCAAGACCATCGACGGCCGCGGCTACAGCGTTCACGTCACCGGTGGCGGATGCATAACCCTCCAGTACGTCTCCAACATCATTATCCACAACATCCACGTCCACCACTGCAAGCCCGCCGGCAACACCAACGTCGCCTCCAGCCCCACCCACGTCGGCTGGCGCGGCGAGTCGGACGGCGACGGCATCTCCCTCTTCAGTGCTCACAAAATCTGGATCGACCACTGCTCCCTCTCGTTCTGCGCTGACGGATTAATCGACGCCATCATGGGGTCGACCGGAATCACGATTTCCAACAACTACTTCGCCCACCATGACGAGGTGATGCTGTTGGGCCACAACGACAAGTACTTGCCGGACTCCGGGATGCAGGTGACGATAGCGTTTAACCACTTCGGTGTGGCGCTGGTGCAGCGTATGCCGCGGTGCAGACGCGGGTACATCCACGTGGTGAACAACGACTTCACGCGGTGGGAAATGTATGCTATCGGCGGTAGCGCTAACCCCACCATCAACAGCCAGGGGAACCGGTACACTGCTCCTCAGGACACGAACGCCAAGGAGGTGACAAAGCGCGTGGACACGAACGAGGGGGATTGGTCCGACTGGAACTGGAGGACGGAGGGGGACATAATGGTAAATGGCGCGTTTTTCGTACCGTCAGGCGCGGGAATGAGCGCTCAGTACGCCAAGGCCTCAAGCATCGAGCCCAAGTCCGTGACTCTCATTGAACAAATCACGGCCAACGCCGGTGTCTTTGGTGACCCAAGGGAGGAGAGCAGTCGCGTAACGAGCCCCGACGACGGGCCCATCACCGACGGCGGCAGCACCGGCAGTGGCAGCGGAGGATCCAACGGCCACGGCAACGGCAATGGCGATGACTATTTTGGATTGATATTTGGGAACGGTGCTCGACCAAGCCCAAACACAATATTCTTGTCTATTTCAATTATTTTTATTTTGTACTTTACCACCAACCATGGTGGTGCTCTATTATCTTTACCATTATTATTAACATTATTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002376 GO:0003674 GO:0003824 GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0009605 GO:0009607 GO:0009814 GO:0009825 GO:0009987 GO:0016020 GO:0016043 GO:0016049 GO:0016829 GO:0016835 GO:0016837 GO:0030570 GO:0031224 GO:0031225 GO:0031226 GO:0040007 GO:0042545 GO:0042547 GO:0043207 GO:0044425 GO:0044459 GO:0044464 GO:0045087 GO:0045229 GO:0046658 GO:0050896 GO:0051704 GO:0051707 GO:0071554 GO:0071555 GO:0071840 GO:0071944 GO:0098542

KEGG Pathways

Metabolic & Signaling

ko00040 ko02024 map00040 map02024

Pfam Domains

Protein Families

Pec_lyase_C

Protein Analysis

499

Amino Acids

53.94

Weight (kDa)

5.69

Isoelectric Point (pI)

32.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pectate_lyase_4	PF00544	152 - 353	1.1e-19	Pectate lyase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006501)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G54920 AT5G04310 AT5G04310 AT5G04310
fragaria_vesca	FvH4_7g18400 FvH4_7g18400 FvH4_7g18400 FvH4_7g18400 FvH4_7g18400
malus_domestica	MD01G1100600.v1.1
prunus_persica	Prupe.2G206100_v2.0.a1 Prupe.2G206100_v2.0.a1
pyrus_communis	pycom01g12750 pycom07g16230
rosa_chinensis	RchiOBHm_Chr1g0362211
rosa_laevigata	RLG00000027666
rosa_multiflora	Rmu_co8314571.1_g000001 Rmu_sc0001237.1_g000010
rosa_roxburghii	Rroxscaffold_4G00294430
rosa_rugosa	Rorug01G0295600 Rorug01G0295700 Rorug01G0295800.1
rosa_samantha	Rh1AG305100 Rh1BG267900 Rh1CG286100 Rh1DG297900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	26, 832
AatII	GACGTC	1 cut(s)	183
Acc36I	ACCTGC	2 cut(s)	26, 832
AccB1I	GGYRCC	1 cut(s)	159
AccI	GTMKAC	1 cut(s)	746
AccII	CGCG	9 cut(s)	492, 636, 895, 906, 940, 1047, 1118, 1139, 1257
AclWI	GGATC	4 cut(s)	422, 695, 1314, 1327
AcoI	YGGCCR	4 cut(s)	357, 487, 1212, 1327
AcuI	CTGAAG	1 cut(s)	652
AcvI	CACGTG	1 cut(s)	919
AcyI	GRCGYC	4 cut(s)	160, 180, 297, 732
AdeI	CACNNNGTG	2 cut(s)	922, 942
AfaI	GTAC	7 cut(s)	539, 821, 911, 1004, 1128, 1157, 1436
AfeI	AGCGCT	2 cut(s)	970, 1150
AfiI	CCNNNNNNNGG	8 cut(s)	370, 371, 741, 790, 833, 1243, 1274, 1275
AgeI	ACCGGT	2 cut(s)	512, 999
AgsI	TTSAA	6 cut(s)	76, 131, 398, 475, 1202, 1419
AjiI	CACGTC	3 cut(s)	508, 571, 625
AjnI	CCWGG	2 cut(s)	155, 990
AjuI	GAANNNNNNNTTGG	2 cut(s)	758, 790
AleI	CACNNNNGTG	1 cut(s)	867
AluBI	AGCT	5 cut(s)	62, 265, 445, 457, 471
AluI	AGCT	5 cut(s)	62, 265, 445, 457, 471
Alw21I	GWGCWC	3 cut(s)	676, 1384, 1462
Alw26I	GTCTC	1 cut(s)	547
AlwI	GGATC	4 cut(s)	422, 695, 1314, 1327
AlwNI	CAGNNNCTG	1 cut(s)	107
Aor51HI	AGCGCT	2 cut(s)	970, 1150
AoxI	GGCC	8 cut(s)	316, 357, 487, 805, 1164, 1212, 1277, 1327
ApaI	GGGCCC	1 cut(s)	1281
ApeKI	GCWGC	4 cut(s)	234, 883, 1296, 1311
AseI	ATTAAT	1 cut(s)	725
AsiGI	ACCGGT	2 cut(s)	512, 999
AspS9I	GGNCC	6 cut(s)	187, 316, 805, 1071, 1277, 1278
AsuC2I	CCSGG	3 cut(s)	356, 372, 835
AsuHPI	GGTGA	9 cut(s)	83, 325, 502, 805, 856, 934, 1048, 1244, 1276
AvaII	GGWCC	2 cut(s)	187, 1071
AxyI	CCTNAGG	1 cut(s)	1014
BaeGI	GKGCMC	2 cut(s)	377, 1281
BamHI	GGATCC	1 cut(s)	1319
BanI	GGYRCC	1 cut(s)	159
BanII	GRGCYC	4 cut(s)	408, 1182, 1268, 1281
BbrPI	CACGTG	1 cut(s)	919
Bbv12I	GWGCWC	3 cut(s)	676, 1384, 1462
BbvI	GCAGC	4 cut(s)	221, 895, 1308, 1323
BccI	CCATC	5 cut(s)	446, 488, 743, 989, 1289
BceAI	ACGGC	9 cut(s)	374, 502, 664, 670, 1227, 1306, 1342, 1348, 1354
BcgI	CGANNNNNNTGC	4 cut(s)	199, 233, 268, 302
BciT130I	CCWGG	2 cut(s)	157, 992
BcnI	CCSGG	3 cut(s)	356, 372, 835
BcoDI	GTCTC	1 cut(s)	547
BfmI	CTRYAG	1 cut(s)	496
BfoI	RGCGCY	5 cut(s)	163, 235, 878, 972, 1152
BfuAI	ACCTGC	2 cut(s)	26, 832
BglI	GCCNNNNNGGC	1 cut(s)	207
BmcAI	AGTACT	1 cut(s)	821
Bme1390I	CCNGG	5 cut(s)	157, 356, 372, 835, 992
Bme18I	GGWCC	2 cut(s)	187, 1071
BmgBI	CACGTC	3 cut(s)	508, 571, 625
BmgT120I	GGNCC	6 cut(s)	187, 316, 805, 1071, 1277, 1278
BmiI	GGNNCC	5 cut(s)	161, 317, 998, 1279, 1321
BmrFI	CCNGG	5 cut(s)	157, 356, 372, 835, 992
BmsI	GCATC	7 cut(s)	100, 288, 512, 666, 786, 828, 1182
BpmI	CTGGAG	4 cut(s)	265, 518, 595, 1105
BpuEI	CTTGAG	1 cut(s)	1153
BpuMI	CCSGG	3 cut(s)	356, 372, 835
BsaAI	YACGTR	1 cut(s)	919
BsaHI	GRCGYC	4 cut(s)	160, 180, 297, 732
BsaJI	CCNNGG	9 cut(s)	300, 490, 893, 991, 1029, 1161, 1238, 1330, 1450
BsaWI	WCCGGW	3 cut(s)	512, 749, 999
BsaXI	ACNNNNNCTCC	6 cut(s)	593, 623, 644, 674, 683, 713
Bsc4I	CCNNNNNNNGG	8 cut(s)	370, 371, 741, 790, 833, 1243, 1274, 1275
Bse118I	RCCGGY	5 cut(s)	512, 590, 999, 1220, 1301
Bse1I	ACTGG	5 cut(s)	196, 248, 535, 1082, 1088
Bse21I	CCTNAGG	1 cut(s)	1014
Bse3DI	GCAATG	1 cut(s)	1348
BseBI	CCWGG	2 cut(s)	157, 992
BseDI	CCNNGG	9 cut(s)	300, 490, 893, 991, 1029, 1161, 1238, 1330, 1450
BseGI	GGATG	4 cut(s)	527, 564, 843, 912
BseLI	CCNNNNNNNGG	8 cut(s)	370, 371, 741, 790, 833, 1243, 1274, 1275
BseMI	GCAATG	1 cut(s)	1348
BseMII	CTCAG	2 cut(s)	1028, 1166
BseNI	ACTGG	5 cut(s)	196, 248, 535, 1082, 1088
BseRI	GAGGAG	3 cut(s)	39, 1002, 1259
BseSI	GKGCMC	2 cut(s)	377, 1281
BseX3I	CGGCCG	2 cut(s)	357, 487
BseXI	GCAGC	4 cut(s)	221, 895, 1308, 1323
BsgI	GTGCAG	2 cut(s)	902, 919
Bsh1236I	CGCG	9 cut(s)	492, 636, 895, 906, 940, 1047, 1118, 1139, 1257
Bsh1285I	CGRYCG	3 cut(s)	360, 490, 750
BshFI	GGCC	8 cut(s)	318, 359, 489, 807, 1166, 1214, 1279, 1329
BshNI	GGYRCC	1 cut(s)	159
BshTI	ACCGGT	2 cut(s)	512, 999
BsiEI	CGRYCG	3 cut(s)	360, 490, 750
BsiHKAI	GWGCWC	3 cut(s)	676, 1384, 1462
BslFI	GGGAC	1 cut(s)	1112
BslI	CCNNNNNNNGG	8 cut(s)	370, 371, 741, 790, 833, 1243, 1274, 1275
BsmAI	GTCTC	1 cut(s)	547
BsmBI	CGTCTC	1 cut(s)	547
BsmFI	GGGAC	1 cut(s)	1112
BsmI	GAATGC	1 cut(s)	21
BsnI	GGCC	8 cut(s)	318, 359, 489, 807, 1166, 1214, 1279, 1329
Bsp120I	GGGCCC	1 cut(s)	1277
Bsp1286I	GDGCHC	8 cut(s)	377, 408, 676, 1182, 1268, 1281, 1384, 1462
Bsp143I	GATC	4 cut(s)	321, 414, 687, 1319
Bsp19I	CCATGG	2 cut(s)	300, 1450
BspANI	GGCC	8 cut(s)	318, 359, 489, 807, 1166, 1214, 1279, 1329
BspCNI	CTCAG	2 cut(s)	1027, 1165
BspFNI	CGCG	9 cut(s)	492, 636, 895, 906, 940, 1047, 1118, 1139, 1257
BspHI	TCATGA	1 cut(s)	121
BspLI	GGNNCC	5 cut(s)	161, 317, 998, 1279, 1321
BspMI	ACCTGC	2 cut(s)	26, 832
BspPI	GGATC	4 cut(s)	422, 695, 1314, 1327
BspT107I	GGYRCC	1 cut(s)	159
BsrDI	GCAATG	1 cut(s)	1348
BsrFI	RCCGGY	5 cut(s)	512, 590, 999, 1220, 1301
BsrI	ACTGG	5 cut(s)	196, 248, 535, 1082, 1088
BssAI	RCCGGY	5 cut(s)	512, 590, 999, 1220, 1301
BssECI	CCNNGG	9 cut(s)	300, 490, 893, 991, 1029, 1161, 1238, 1330, 1450
BssMI	GATC	4 cut(s)	321, 414, 687, 1319
BssNI	GRCGYC	4 cut(s)	160, 180, 297, 732
BssT1I	CCWWGG	5 cut(s)	300, 1029, 1161, 1238, 1450
Bst2UI	CCWGG	2 cut(s)	157, 992
Bst4CI	ACNGT	2 cut(s)	1131, 1379
Bst6I	CTCTTC	1 cut(s)	671
BstACI	GRCGYC	4 cut(s)	160, 180, 297, 732
BstAPI	GCANNNNNTGC	1 cut(s)	889
BstBAI	YACGTR	1 cut(s)	919
BstC8I	GCNNGC	8 cut(s)	230, 267, 377, 455, 584, 588, 592, 632
BstDEI	CTNAG	2 cut(s)	1014, 1152
BstDSI	CCRYGG	5 cut(s)	300, 490, 893, 1330, 1450
BstEII	GGTNACC	1 cut(s)	1232
BstF5I	GGATG	4 cut(s)	527, 564, 843, 912
BstFNI	CGCG	9 cut(s)	492, 636, 895, 906, 940, 1047, 1118, 1139, 1257
BstH2I	RGCGCY	5 cut(s)	163, 235, 878, 972, 1152
BstKTI	GATC	4 cut(s)	324, 417, 690, 1322
BstMAI	GTCTC	1 cut(s)	547
BstMBI	GATC	4 cut(s)	321, 414, 687, 1319
BstMCI	CGRYCG	3 cut(s)	360, 490, 750
BstNI	CCWGG	2 cut(s)	157, 992
BstPI	GGTNACC	1 cut(s)	1232
BstSCI	CCNGG	5 cut(s)	155, 354, 370, 833, 990
BstSFI	CTRYAG	1 cut(s)	496
BstSLI	GKGCMC	2 cut(s)	377, 1281
BstUI	CGCG	9 cut(s)	492, 636, 895, 906, 940, 1047, 1118, 1139, 1257
BstV1I	GCAGC	4 cut(s)	221, 895, 1308, 1323
BstX2I	RGATCY	1 cut(s)	1319
BstYI	RGATCY	1 cut(s)	1319
BstZI	CGGCCG	2 cut(s)	357, 487
Bsu36I	CCTNAGG	1 cut(s)	1014
BsuRI	GGCC	8 cut(s)	318, 359, 489, 807, 1166, 1214, 1279, 1329
BtgI	CCRYGG	5 cut(s)	300, 490, 893, 1330, 1450
BtgZI	GCGATG	1 cut(s)	1362
BtrI	CACGTC	3 cut(s)	508, 571, 625
BtsCI	GGATG	4 cut(s)	527, 564, 843, 912
BtsI	GCAGTG	4 cut(s)	577, 694, 1005, 1312
BtsIMutI	CAGTG	6 cut(s)	203, 577, 676, 694, 1005, 1312
BveI	ACCTGC	2 cut(s)	26, 832
Cac8I	GCNNGC	8 cut(s)	230, 267, 377, 455, 584, 588, 592, 632
CaiI	CAGNNNCTG	1 cut(s)	107
CciI	TCATGA	1 cut(s)	121
Cfr10I	RCCGGY	5 cut(s)	512, 590, 999, 1220, 1301
Cfr13I	GGNCC	6 cut(s)	187, 316, 805, 1071, 1277, 1278
Cfr42I	CCGCGG	2 cut(s)	493, 896
CseI	GACGC	4 cut(s)	135, 305, 740, 912
Csp6I	GTAC	7 cut(s)	538, 820, 910, 1003, 1127, 1156, 1435
CspAI	ACCGGT	2 cut(s)	512, 999
CspCI	CAANNNNNGTGG	4 cut(s)	1029, 1064, 1435, 1470
CviAII	CATG	7 cut(s)	122, 292, 301, 433, 739, 785, 1451
CviQI	GTAC	7 cut(s)	538, 820, 910, 1003, 1127, 1156, 1435
DdeI	CTNAG	2 cut(s)	1014, 1152
DinI	GGCGCC	1 cut(s)	161
DpnI	GATC	4 cut(s)	323, 416, 689, 1321
DpnII	GATC	4 cut(s)	321, 414, 687, 1319
DraIII	CACNNNGTG	2 cut(s)	922, 942
EaeI	YGGCCR	4 cut(s)	357, 487, 1212, 1327
EagI	CGGCCG	2 cut(s)	357, 487
Eam1104I	CTCTTC	1 cut(s)	671
EarI	CTCTTC	1 cut(s)	671
EciI	GGCGGA	2 cut(s)	371, 534
EclXI	CGGCCG	2 cut(s)	357, 487
Eco130I	CCWWGG	5 cut(s)	300, 1029, 1161, 1238, 1450
Eco147I	AGGCCT	1 cut(s)	1166
Eco24I	GRGCYC	4 cut(s)	408, 1182, 1268, 1281
Eco47I	GGWCC	2 cut(s)	187, 1071
Eco47III	AGCGCT	2 cut(s)	970, 1150
Eco52I	CGGCCG	2 cut(s)	357, 487
Eco57I	CTGAAG	1 cut(s)	652
Eco72I	CACGTG	1 cut(s)	919
Eco81I	CCTNAGG	1 cut(s)	1014
Eco91I	GGTNACC	1 cut(s)	1232
EcoO109I	RGGNCCY	1 cut(s)	316
EcoO65I	GGTNACC	1 cut(s)	1232
EcoRII	CCWGG	2 cut(s)	155, 990
EcoT14I	CCWWGG	5 cut(s)	300, 1029, 1161, 1238, 1450
EcoT22I	ATGCAT	1 cut(s)	527
EcoT38I	GRGCYC	4 cut(s)	408, 1182, 1268, 1281
EgeI	GGCGCC	1 cut(s)	161
EheI	GGCGCC	1 cut(s)	161
ErhI	CCWWGG	5 cut(s)	300, 1029, 1161, 1238, 1450
Esp3I	CGTCTC	1 cut(s)	547
FaeI	CATG	7 cut(s)	125, 295, 304, 436, 742, 788, 1454
FaqI	GGGAC	1 cut(s)	1112
FatI	CATG	7 cut(s)	121, 291, 300, 432, 738, 784, 1450
FauI	CCCGC	3 cut(s)	595, 899, 1132
FblI	GTMKAC	1 cut(s)	746
FokI	GGATG	4 cut(s)	534, 551, 850, 899
FriOI	GRGCYC	4 cut(s)	408, 1182, 1268, 1281
GsuI	CTGGAG	4 cut(s)	265, 518, 595, 1105
HaeII	RGCGCY	5 cut(s)	163, 235, 878, 972, 1152
HaeIII	GGCC	8 cut(s)	318, 359, 489, 807, 1166, 1214, 1279, 1329
HgaI	GACGC	4 cut(s)	135, 305, 740, 912
Hin1I	GRCGYC	4 cut(s)	160, 180, 297, 732
Hin1II	CATG	7 cut(s)	125, 295, 304, 436, 742, 788, 1454
HincII	GTYRAC	2 cut(s)	466, 747
HindII	GTYRAC	2 cut(s)	466, 747
HindIII	AAGCTT	1 cut(s)	60
HinfI	GANTC	7 cut(s)	138, 338, 347, 641, 753, 830, 1192
HphI	GGTGA	9 cut(s)	83, 325, 502, 805, 856, 934, 1048, 1244, 1276
Hpy166II	GTNNAC	7 cut(s)	466, 505, 574, 747, 925, 1005, 1051
Hpy188I	TCNGA	3 cut(s)	346, 646, 1075
Hpy188III	TCNNGA	6 cut(s)	122, 412, 475, 685, 757, 1016
Hpy8I	GTNNAC	7 cut(s)	466, 505, 574, 747, 925, 1005, 1051
HpyAV	CCTTC	1 cut(s)	82
HpyCH4III	ACNGT	2 cut(s)	1131, 1379
HpyCH4IV	ACGT	7 cut(s)	180, 507, 540, 570, 603, 624, 918
HpyCH4V	TGCA	7 cut(s)	21, 113, 525, 582, 841, 883, 900
HpyF3I	CTNAG	2 cut(s)	1014, 1152
HpySE526I	ACGT	7 cut(s)	180, 507, 540, 570, 603, 624, 918
Hsp92I	GRCGYC	4 cut(s)	160, 180, 297, 732
Hsp92II	CATG	7 cut(s)	125, 295, 304, 436, 742, 788, 1454
KasI	GGCGCC	1 cut(s)	159
KroI	GCCGGC	1 cut(s)	590
KroNI	GCCGGC	1 cut(s)	592
KspI	CCGCGG	2 cut(s)	493, 896
Kzo9I	GATC	4 cut(s)	321, 414, 687, 1319
LmnI	GCTCC	4 cut(s)	246, 384, 704, 1015
Lsp1109I	GCAGC	4 cut(s)	221, 895, 1308, 1323
LweI	GCATC	7 cut(s)	100, 288, 512, 666, 786, 828, 1182
MaeII	ACGT	7 cut(s)	180, 507, 540, 570, 603, 624, 918
MaeIII	GTNAC	7 cut(s)	331, 508, 844, 1036, 1189, 1232, 1258
MalI	GATC	4 cut(s)	323, 416, 689, 1321
MboI	GATC	4 cut(s)	321, 414, 687, 1319
MboII	GAAGA	2 cut(s)	412, 658
MflI	RGATCY	1 cut(s)	1319
MhlI	GDGCHC	8 cut(s)	377, 408, 676, 1182, 1268, 1281, 1384, 1462
MluCI	AATT	2 cut(s)	30, 1419
Mly113I	GGCGCC	1 cut(s)	160
MlyI	GAGTC	5 cut(s)	147, 332, 650, 824, 1186
MmeI	TCCRAC	7 cut(s)	324, 375, 570, 624, 789, 1098, 1347
Mph1103I	ATGCAT	1 cut(s)	527
MroNI	GCCGGC	1 cut(s)	590
MseI	TTAA	4 cut(s)	29, 725, 858, 1484
MslI	CAYNNNNRTG	3 cut(s)	867, 917, 1455
MspA1I	CMGCKG	3 cut(s)	492, 895, 1314
MspR9I	CCNGG	5 cut(s)	157, 356, 372, 835, 992
Mva1269I	GAATGC	1 cut(s)	21
MvaI	CCWGG	2 cut(s)	157, 992
MvnI	CGCG	9 cut(s)	492, 636, 895, 906, 940, 1047, 1118, 1139, 1257
NaeI	GCCGGC	1 cut(s)	592
NarI	GGCGCC	1 cut(s)	160
NciI	CCSGG	3 cut(s)	356, 372, 835
NcoI	CCATGG	2 cut(s)	300, 1450
NdeII	GATC	4 cut(s)	321, 414, 687, 1319
NgoMIV	GCCGGC	1 cut(s)	590
NlaIII	CATG	7 cut(s)	125, 295, 304, 436, 742, 788, 1454
NlaIV	GGNNCC	5 cut(s)	161, 317, 998, 1279, 1321
NmuCI	GTSAC	6 cut(s)	331, 508, 844, 1036, 1189, 1232
NsiI	ATGCAT	1 cut(s)	527
OliI	CACNNNNGTG	1 cut(s)	867
PagI	TCATGA	1 cut(s)	121
PaqCI	CACCTGC	2 cut(s)	26, 832
PceI	AGGCCT	1 cut(s)	1166
PcsI	WCGNNNNNNNCGW	2 cut(s)	726, 1344
PctI	GAATGC	1 cut(s)	21
PdiI	GCCGGC	1 cut(s)	592
PfeI	GAWTC	2 cut(s)	347, 753
PfoI	TCCNGGA	1 cut(s)	833
PinAI	ACCGGT	2 cut(s)	512, 999
PleI	GAGTC	5 cut(s)	146, 332, 649, 824, 1186
PluTI	GGCGCC	1 cut(s)	163
PmaCI	CACGTG	1 cut(s)	919
PmlI	CACGTG	1 cut(s)	919
PpsI	GAGTC	5 cut(s)	146, 332, 649, 824, 1186
Ppu21I	YACGTR	1 cut(s)	919
PshBI	ATTAAT	1 cut(s)	725
Psp6I	CCWGG	2 cut(s)	155, 990
PspCI	CACGTG	1 cut(s)	919
PspEI	GGTNACC	1 cut(s)	1232
PspGI	CCWGG	2 cut(s)	155, 990
PspN4I	GGNNCC	5 cut(s)	161, 317, 998, 1279, 1321
PspOMI	GGGCCC	1 cut(s)	1277
PspPI	GGNCC	6 cut(s)	187, 316, 805, 1071, 1277, 1278
PstNI	CAGNNNCTG	1 cut(s)	107
PsuI	RGATCY	1 cut(s)	1319
RsaI	GTAC	7 cut(s)	539, 821, 911, 1004, 1128, 1157, 1436
RsaNI	GTAC	7 cut(s)	538, 820, 910, 1003, 1127, 1156, 1435
RseI	CAYNNNNRTG	3 cut(s)	867, 917, 1455
SacII	CCGCGG	2 cut(s)	493, 896
SalI	GTCGAC	1 cut(s)	745
SaqAI	TTAA	4 cut(s)	29, 725, 858, 1484
Sau3AI	GATC	4 cut(s)	321, 414, 687, 1319
Sau96I	GGNCC	6 cut(s)	187, 316, 805, 1071, 1277, 1278
ScaI	AGTACT	1 cut(s)	821
SchI	GAGTC	5 cut(s)	147, 332, 650, 824, 1186
ScrFI	CCNGG	5 cut(s)	157, 356, 372, 835, 992
SduI	GDGCHC	8 cut(s)	377, 408, 676, 1182, 1268, 1281, 1384, 1462
SfaNI	GCATC	7 cut(s)	100, 288, 512, 666, 786, 828, 1182
SfcI	CTRYAG	1 cut(s)	496
SfoI	GGCGCC	1 cut(s)	161
Sfr303I	CCGCGG	2 cut(s)	493, 896
SgrAI	CRCCGGYG	2 cut(s)	512, 1220
SgrBI	CCGCGG	2 cut(s)	493, 896
SinI	GGWCC	2 cut(s)	187, 1071
SmiMI	CAYNNNNRTG	3 cut(s)	867, 917, 1455
SmlI	CTYRAG	1 cut(s)	1168
SmoI	CTYRAG	1 cut(s)	1168
Sse9I	AATT	2 cut(s)	30, 1419
SseBI	AGGCCT	1 cut(s)	1166
SspDI	GGCGCC	1 cut(s)	159
SspI	AATATT	1 cut(s)	1404
StuI	AGGCCT	1 cut(s)	1166
StyD4I	CCNGG	5 cut(s)	155, 354, 370, 833, 990
StyI	CCWWGG	5 cut(s)	300, 1029, 1161, 1238, 1450
TaaI	ACNGT	2 cut(s)	1131, 1379
TaiI	ACGT	7 cut(s)	183, 510, 543, 573, 606, 627, 921
TaqI	TCGA	7 cut(s)	219, 483, 690, 729, 746, 1176, 1384
TasI	AATT	2 cut(s)	30, 1419
TatI	WGTACW	2 cut(s)	819, 1434
TauI	GCSGC	6 cut(s)	279, 492, 495, 639, 895, 1296
TfiI	GAWTC	2 cut(s)	347, 753
Tru1I	TTAA	4 cut(s)	29, 725, 858, 1484
Tru9I	TTAA	4 cut(s)	29, 725, 858, 1484
TscAI	CASTG	6 cut(s)	203, 584, 676, 701, 1012, 1312
TseFI	GTSAC	6 cut(s)	331, 508, 844, 1036, 1189, 1232
TseI	GCWGC	4 cut(s)	234, 883, 1296, 1311
Tsp45I	GTSAC	6 cut(s)	331, 508, 844, 1036, 1189, 1232
TspDTI	ATGAA	1 cut(s)	138
TspGWI	ACGGA	3 cut(s)	735, 1106, 1177
TspRI	CASTG	6 cut(s)	203, 584, 676, 701, 1012, 1312
VpaK11BI	GGWCC	2 cut(s)	187, 1071
VspI	ATTAAT	1 cut(s)	725
XmiI	GTMKAC	1 cut(s)	746
ZraI	GACGTC	1 cut(s)	181
ZrmI	AGTACT	1 cut(s)	821
Zsp2I	ATGCAT	1 cut(s)	527

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.