Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0011656

Physical Location & Seq

Genomic Coordinates Reverse (-)

2274260 .. 2276039

Gene Structure 1780 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G07250.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1041 bp

ATGGCTGTCGGAGATGATGATCTGGAGAACAGAATGTCGGCGAAAGATCGAGGAATCGGAAGCAGAGGCGGTGATCGGAATCGAATCGGACCACCGCCTCTGCCGGTAGCCTTGTCGTCGTCTACTGAATTTGGCGATAACGCGTTGTCGTCGCGGTGGACGTCGTGGCTTGTTCCGATGTTCGTTGTGGCTAACGTTGCGGTTTTCGTCGTGGCGATGTTCGTTAATAACTGTCCGAATCATTTCGAGTCTCACCGTCTCAGAGGTCACTGTGTCGCCAAGTTTCTCGGTAGACTCTCCTTTGAGCCTCTCCGAACTAATCCTCTCTTTGGTCCATCTTCTCACACATTGGAGAAGTTAGGAGCACTTGAGTGGAGCAAAGTTGTGGAGAAGAAGGAAGGTTGGCGTCTCTTGACATGTATATGGTTACACGCGGGTGTTATTCATCTCGGAGCCAATATGTTAAGCCTTGTTTTCATTGGTATTCGTCTCGAGCAGCAATTTGGTTTTGTTAGAATCGGAGTTATATACCTATTATCAGGAATTGGAGGGAGTGTATTGTCGTCGTTGTTTATCAGAAACAGTATCTCTGTTGGAGCTTCTGGTGCTCTTTTTGGCCTCCTTGGCTCAATGCTCTCTGAACTTTTCACCAACTGGACAATCTACTCTAACAAGATTGCTGCACTCCTGACGCTTTTGTTTGTCATCTTGATAAACTTGGCAATTGGGATTCTGCCTCACGTTGATAATTTTGCTCATGTGGGTGGATTTGTAACGGGGTTTCTCCTCGGTTTCATTCTGTTGGCTCGTCCCCAGTTTAAGTGGTTAGCAAGAGAGCATATGCCACAAGGCACACCTCTGAGATACAAGTACAAGACTTACCAATACCTATTGTGGCTCCTATCTTTGGTTCTATTGATTGCCGGGTTTGTGGTGGCGCTGTTGATGCTATTTCGAGGAGAAAACGGCAATGATCACTGCCGTTGGTGTCACTACCTGCGCTGTGTTCCCACCTCAAGTTGGAGGTGTGATGATGTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0002791 GO:0002792 GO:0003674 GO:0003824 GO:0004175 GO:0004252 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005794 GO:0005911 GO:0006508 GO:0006807 GO:0006928 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0008283 GO:0009506 GO:0009894 GO:0009966 GO:0009987 GO:0010646 GO:0012505 GO:0016020 GO:0016477 GO:0016787 GO:0017171 GO:0019222 GO:0019538 GO:0023051 GO:0030054 GO:0030162 GO:0031090 GO:0031323 GO:0031329 GO:0031984 GO:0032268 GO:0032879 GO:0032880 GO:0040011 GO:0042058 GO:0042175 GO:0042176 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0048519 GO:0048523 GO:0048583 GO:0048870 GO:0050708 GO:0050709 GO:0050789 GO:0050794 GO:0051046 GO:0051048 GO:0051049 GO:0051051 GO:0051171 GO:0051179 GO:0051223 GO:0051224 GO:0051246 GO:0051674 GO:0055044 GO:0060255 GO:0061136 GO:0065007 GO:0070011 GO:0070201 GO:0071704 GO:0080090 GO:0090087 GO:0098588 GO:0098791 GO:0098827 GO:0140096 GO:1901184 GO:1901564 GO:1903050 GO:1903362 GO:1903530 GO:1903531 GO:1904950

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Rhomboid

Protein Analysis

346

Amino Acids

38.49

Weight (kDa)

9.35

Isoelectric Point (pI)

29.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Rhomboid	PF01694	132 - 269	5.5e-44	Rhomboid domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011656)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G07250 AT5G07250
fragaria_vesca	FvH4_7g29110 FvH4_7g29110
malus_domestica	MD01G1198000.v1.1 MD07G1265600.v1.1
prunus_persica	Prupe.2G291300_v2.0.a1
pyrus_communis	pycom01g20810 pycom07g24380
rosa_chinensis	RchiOBHm_Chr1g0376861
rosa_laevigata	RLG00000026564
rosa_multiflora	Rmu_sc0002113.1_g000004
rosa_roxburghii	Rroxscaffold_4G00281670
rosa_rugosa	Rorug01G0399100
rosa_samantha	Rh1AG416300 Rh1BG375600 Rh1CG389400 Rh1DG406200
rosa_wichuraiana	Rw1G036520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	164
Acc36I	ACCTGC	1 cut(s)	1005
AccI	GTMKAC	2 cut(s)	122, 292
AccII	CGCG	3 cut(s)	143, 154, 434
AciI	CCGC	5 cut(s)	69, 95, 154, 200, 434
AclI	AACGTT	1 cut(s)	195
AcsI	RAATTY	1 cut(s)	128
AcyI	GRCGYC	2 cut(s)	161, 406
AfaI	GTAC	1 cut(s)	872
AfiI	CCNNNNNNNGG	3 cut(s)	329, 907, 1020
AflIII	ACRYGT	2 cut(s)	141, 416
AleI	CACNNNNGTG	2 cut(s)	370, 435
AluBI	AGCT	1 cut(s)	599
AluI	AGCT	1 cut(s)	599
Alw21I	GWGCWC	2 cut(s)	367, 610
Alw26I	GTCTC	4 cut(s)	255, 263, 413, 494
Ama87I	CYCGRG	1 cut(s)	491
AoxI	GGCC	1 cut(s)	616
ApeKI	GCWGC	2 cut(s)	496, 680
ApoI	RAATTY	1 cut(s)	128
Asp700I	GAANNNNTTC	1 cut(s)	242
AspLEI	GCGC	2 cut(s)	940, 1002
AspS9I	GGNCC	2 cut(s)	89, 332
AsuC2I	CCSGG	1 cut(s)	925
AsuHPI	GGTGA	3 cut(s)	83, 245, 640
AvaI	CYCGRG	1 cut(s)	491
AvaII	GGWCC	2 cut(s)	89, 332
Bbv12I	GWGCWC	2 cut(s)	367, 610
BbvI	GCAGC	2 cut(s)	508, 667
BccI	CCATC	1 cut(s)	343
BceAI	ACGGC	2 cut(s)	966, 982
BclI	TGATCA	1 cut(s)	973
BcnI	CCSGG	1 cut(s)	925
BcoDI	GTCTC	4 cut(s)	255, 263, 413, 494
BfoI	RGCGCY	1 cut(s)	941
BfuAI	ACCTGC	1 cut(s)	1005
BglI	GCCNNNNNGGC	1 cut(s)	624
BisI	GCNGC	2 cut(s)	497, 681
BlsI	GCNGC	2 cut(s)	498, 682
Bme1390I	CCNGG	1 cut(s)	925
Bme18I	GGWCC	2 cut(s)	89, 332
BmeT110I	CYCGRG	1 cut(s)	491
BmgT120I	GGNCC	2 cut(s)	89, 332
BmiI	GGNNCC	2 cut(s)	454, 899
BmrFI	CCNGG	1 cut(s)	925
BmrI	ACTGGG	1 cut(s)	808
BmsI	GCATC	1 cut(s)	936
BmuI	ACTGGG	1 cut(s)	808
BpmI	CTGGAG	1 cut(s)	44
BpuEI	CTTGAG	2 cut(s)	389, 1000
BpuMI	CCSGG	1 cut(s)	925
BsaBI	GATNNNNATC	2 cut(s)	18, 78
BsaHI	GRCGYC	2 cut(s)	161, 406
BsaJI	CCNNGG	2 cut(s)	622, 787
BsaXI	ACNNNNNCTCC	2 cut(s)	540, 570
Bsc4I	CCNNNNNNNGG	3 cut(s)	329, 907, 1020
Bse118I	RCCGGY	1 cut(s)	103
Bse1I	ACTGG	2 cut(s)	659, 814
Bse3DI	GCAATG	1 cut(s)	976
Bse8I	GATNNNNATC	2 cut(s)	18, 78
BseDI	CCNNGG	2 cut(s)	622, 787
BseJI	GATNNNNATC	2 cut(s)	18, 78
BseLI	CCNNNNNNNGG	3 cut(s)	329, 907, 1020
BseMI	GCAATG	1 cut(s)	976
BseMII	CTCAG	2 cut(s)	274, 851
BseNI	ACTGG	2 cut(s)	659, 814
BseRI	GAGGAG	2 cut(s)	776, 972
BseXI	GCAGC	2 cut(s)	508, 667
BsgI	GTGCAG	1 cut(s)	666
Bsh1236I	CGCG	3 cut(s)	143, 154, 434
BshFI	GGCC	1 cut(s)	618
BsiHKAI	GWGCWC	2 cut(s)	367, 610
BsiHKCI	CYCGRG	1 cut(s)	491
BsiSI	CCGG	2 cut(s)	104, 924
BslFI	GGGAC	1 cut(s)	795
BslI	CCNNNNNNNGG	3 cut(s)	329, 907, 1020
BsmAI	GTCTC	4 cut(s)	255, 263, 413, 494
BsmBI	CGTCTC	3 cut(s)	263, 413, 494
BsmFI	GGGAC	1 cut(s)	795
BsnI	GGCC	1 cut(s)	618
BsoBI	CYCGRG	1 cut(s)	491
Bsp1286I	GDGCHC	2 cut(s)	367, 610
Bsp143I	GATC	4 cut(s)	19, 46, 73, 973
BspACI	CCGC	5 cut(s)	69, 95, 154, 200, 434
BspANI	GGCC	1 cut(s)	618
BspCNI	CTCAG	2 cut(s)	273, 852
BspFNI	CGCG	3 cut(s)	143, 154, 434
BspLI	GGNNCC	2 cut(s)	454, 899
BspMI	ACCTGC	1 cut(s)	1005
BsrDI	GCAATG	1 cut(s)	976
BsrFI	RCCGGY	1 cut(s)	103
BsrI	ACTGG	2 cut(s)	659, 814
BssAI	RCCGGY	1 cut(s)	103
BssECI	CCNNGG	2 cut(s)	622, 787
BssMI	GATC	4 cut(s)	19, 46, 73, 973
BssNI	GRCGYC	2 cut(s)	161, 406
BssT1I	CCWWGG	1 cut(s)	622
Bst4CI	ACNGT	4 cut(s)	233, 257, 272, 584
BstACI	GRCGYC	2 cut(s)	161, 406
BstDEI	CTNAG	2 cut(s)	260, 860
BstFNI	CGCG	3 cut(s)	143, 154, 434
BstH2I	RGCGCY	1 cut(s)	941
BstHHI	GCGC	2 cut(s)	940, 1002
BstKTI	GATC	4 cut(s)	22, 49, 76, 976
BstMAI	GTCTC	4 cut(s)	255, 263, 413, 494
BstMBI	GATC	4 cut(s)	19, 46, 73, 973
BstMWI	GCNNNNNNNGC	4 cut(s)	197, 605, 624, 946
BstNSI	RCATGY	1 cut(s)	420
BstSCI	CCNGG	1 cut(s)	923
BstUI	CGCG	3 cut(s)	143, 154, 434
BstV1I	GCAGC	2 cut(s)	508, 667
BsuRI	GGCC	1 cut(s)	618
BtgZI	GCGATG	1 cut(s)	230
BtsI	GCAGTG	1 cut(s)	976
BtsIMutI	CAGTG	2 cut(s)	268, 976
BveI	ACCTGC	1 cut(s)	1005
CfoI	GCGC	2 cut(s)	940, 1002
Cfr10I	RCCGGY	1 cut(s)	103
Cfr13I	GGNCC	2 cut(s)	89, 332
CseI	GACGC	2 cut(s)	395, 700
Csp6I	GTAC	1 cut(s)	871
CviAII	CATG	2 cut(s)	417, 758
CviQI	GTAC	1 cut(s)	871
DdeI	CTNAG	2 cut(s)	260, 860
DpnI	GATC	4 cut(s)	21, 48, 75, 975
DpnII	GATC	4 cut(s)	19, 46, 73, 973
Eco130I	CCWWGG	1 cut(s)	622
Eco47I	GGWCC	2 cut(s)	89, 332
Eco88I	CYCGRG	1 cut(s)	491
EcoT14I	CCWWGG	1 cut(s)	622
ErhI	CCWWGG	1 cut(s)	622
Esp3I	CGTCTC	3 cut(s)	263, 413, 494
FaeI	CATG	2 cut(s)	420, 761
FaiI	YATR	9 cut(s)	418, 422, 424, 461, 527, 529, 759, 840, 842
FaqI	GGGAC	1 cut(s)	795
FatI	CATG	2 cut(s)	416, 757
FauI	CCCGC	1 cut(s)	427
FauNDI	CATATG	1 cut(s)	840
FbaI	TGATCA	1 cut(s)	973
FblI	GTMKAC	2 cut(s)	122, 292
Fnu4HI	GCNGC	2 cut(s)	497, 681
Fsp4HI	GCNGC	2 cut(s)	497, 681
GlaI	GCGC	2 cut(s)	939, 1001
GluI	GCNGC	2 cut(s)	497, 681
GsuI	CTGGAG	1 cut(s)	44
HaeII	RGCGCY	1 cut(s)	941
HaeIII	GGCC	1 cut(s)	618
HapII	CCGG	2 cut(s)	104, 924
HgaI	GACGC	2 cut(s)	395, 700
HhaI	GCGC	2 cut(s)	940, 1002
Hin1I	GRCGYC	2 cut(s)	161, 406
Hin1II	CATG	2 cut(s)	420, 761
Hin6I	GCGC	2 cut(s)	938, 1000
HinP1I	GCGC	2 cut(s)	938, 1000
HinfI	GANTC	8 cut(s)	54, 79, 84, 238, 248, 294, 516, 730
HpaII	CCGG	2 cut(s)	104, 924
HphI	GGTGA	3 cut(s)	83, 245, 640
Hpy166II	GTNNAC	3 cut(s)	123, 159, 293
Hpy188III	TCNNGA	6 cut(s)	23, 412, 491, 540, 688, 709
Hpy8I	GTNNAC	3 cut(s)	123, 159, 293
Hpy99I	CGWCG	5 cut(s)	121, 154, 166, 212, 568
HpyAV	CCTTC	2 cut(s)	388, 392
HpyCH4III	ACNGT	4 cut(s)	233, 257, 272, 584
HpyCH4IV	ACGT	3 cut(s)	161, 195, 741
HpyCH4V	TGCA	1 cut(s)	683
HpyF10VI	GCNNNNNNNGC	4 cut(s)	197, 605, 624, 946
HpyF3I	CTNAG	2 cut(s)	260, 860
HpySE526I	ACGT	3 cut(s)	161, 195, 741
Hsp92I	GRCGYC	2 cut(s)	161, 406
Hsp92II	CATG	2 cut(s)	420, 761
HspAI	GCGC	2 cut(s)	938, 1000
Ksp22I	TGATCA	1 cut(s)	973
Kzo9I	GATC	4 cut(s)	19, 46, 73, 973
LmnI	GCTCC	5 cut(s)	362, 375, 452, 596, 903
LpnPI	CCDG	9 cut(s)	8, 117, 525, 588, 640, 701, 827, 937, 1010
Lsp1109I	GCAGC	2 cut(s)	508, 667
LweI	GCATC	1 cut(s)	936
MaeII	ACGT	3 cut(s)	161, 195, 741
MaeIII	GTNAC	4 cut(s)	266, 426, 772, 989
MalI	GATC	4 cut(s)	21, 48, 75, 975
MboI	GATC	4 cut(s)	19, 46, 73, 973
MboII	GAAGA	2 cut(s)	330, 403
MfeI	CAATTG	1 cut(s)	723
MhlI	GDGCHC	2 cut(s)	367, 610
MluCI	AATT	5 cut(s)	128, 500, 543, 723, 748
MluI	ACGCGT	1 cut(s)	141
MlyI	GAGTC	2 cut(s)	257, 288
MmeI	TCCRAC	2 cut(s)	574, 1001
MroXI	GAANNNNTTC	1 cut(s)	242
MseI	TTAA	3 cut(s)	225, 464, 819
MslI	CAYNNNNRTG	4 cut(s)	370, 421, 435, 762
MspI	CCGG	2 cut(s)	104, 924
MspR9I	CCNGG	1 cut(s)	925
MunI	CAATTG	1 cut(s)	723
MvnI	CGCG	3 cut(s)	143, 154, 434
MwoI	GCNNNNNNNGC	4 cut(s)	197, 605, 624, 946
NciI	CCSGG	1 cut(s)	925
NdeI	CATATG	1 cut(s)	840
NdeII	GATC	4 cut(s)	19, 46, 73, 973
NlaIII	CATG	2 cut(s)	420, 761
NlaIV	GGNNCC	2 cut(s)	454, 899
NmuCI	GTSAC	2 cut(s)	266, 989
NspI	RCATGY	1 cut(s)	420
OliI	CACNNNNGTG	2 cut(s)	370, 435
PaeR7I	CTCGAG	1 cut(s)	491
PciI	ACATGT	1 cut(s)	416
PcsI	WCGNNNNNNNCGW	1 cut(s)	158
PdmI	GAANNNNTTC	1 cut(s)	242
PfeI	GAWTC	6 cut(s)	54, 79, 84, 238, 516, 730
PkrI	GCNGC	2 cut(s)	498, 682
PleI	GAGTC	2 cut(s)	256, 288
PpsI	GAGTC	2 cut(s)	256, 288
PscI	ACATGT	1 cut(s)	416
Psp1406I	AACGTT	1 cut(s)	195
PspN4I	GGNNCC	2 cut(s)	454, 899
PspPI	GGNCC	2 cut(s)	89, 332
RsaI	GTAC	1 cut(s)	872
RsaNI	GTAC	1 cut(s)	871
RseI	CAYNNNNRTG	4 cut(s)	370, 421, 435, 762
SaqAI	TTAA	3 cut(s)	225, 464, 819
SatI	GCNGC	2 cut(s)	497, 681
Sau3AI	GATC	4 cut(s)	19, 46, 73, 973
Sau96I	GGNCC	2 cut(s)	89, 332
SchI	GAGTC	2 cut(s)	257, 288
ScrFI	CCNGG	1 cut(s)	925
SduI	GDGCHC	2 cut(s)	367, 610
SfaNI	GCATC	1 cut(s)	936
Sfr274I	CTCGAG	1 cut(s)	491
SinI	GGWCC	2 cut(s)	89, 332
SlaI	CTCGAG	1 cut(s)	491
SmiMI	CAYNNNNRTG	4 cut(s)	370, 421, 435, 762
SmlI	CTYRAG	3 cut(s)	368, 491, 1015
SmoI	CTYRAG	3 cut(s)	368, 491, 1015
Sse9I	AATT	5 cut(s)	128, 500, 543, 723, 748
SsiI	CCGC	5 cut(s)	69, 95, 154, 200, 434
StyD4I	CCNGG	1 cut(s)	923
StyI	CCWWGG	1 cut(s)	622
TaaI	ACNGT	4 cut(s)	233, 257, 272, 584
TaiI	ACGT	3 cut(s)	164, 198, 744
TaqI	TCGA	5 cut(s)	49, 82, 246, 492, 955
TasI	AATT	5 cut(s)	128, 500, 543, 723, 748
TatI	WGTACW	1 cut(s)	870
TfiI	GAWTC	6 cut(s)	54, 79, 84, 238, 516, 730
Tru1I	TTAA	3 cut(s)	225, 464, 819
Tru9I	TTAA	3 cut(s)	225, 464, 819
TscAI	CASTG	2 cut(s)	275, 983
TseFI	GTSAC	2 cut(s)	266, 989
TseI	GCWGC	2 cut(s)	496, 680
Tsp45I	GTSAC	2 cut(s)	266, 989
TspDTI	ATGAA	3 cut(s)	434, 466, 784
TspRI	CASTG	2 cut(s)	275, 983
VpaK11BI	GGWCC	2 cut(s)	89, 332
XapI	RAATTY	1 cut(s)	128
XceI	RCATGY	1 cut(s)	420
XhoI	CTCGAG	1 cut(s)	491
XmiI	GTMKAC	2 cut(s)	122, 292
XmnI	GAANNNNTTC	1 cut(s)	242
ZraI	GACGTC	1 cut(s)	162

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.