Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0011656

Physical Location & Seq

Genomic Coordinates Reverse (-)

29589163 .. 29591746

Gene Structure 2584 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1198000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 984 bp

ATGCCTGCAGAAGATGTGGAAAACAGGGGAGTGAAAAACCGAGGAAGACAGCAGAGCTACTCGTCGTCCTCGTACGCGGTGGATGACGAAGAGGCCCAATGGACTTCTTGGTTGGTGCCAATGATTGTCGTGGCGAATATCGGTGTGTTTCTGGTGGCCATGTACGTCAATAACTGTCCCAAGAACAATTATGCGGCGGCGGAGGGGAAGTGCACGGCGAGGTTTCTTGGGAGGTTGTCGTTTCAGCCGTTGAAGGAGAACCCTCTCTTCGGCCCTTCTTCTTCCACATTAGAGAAGTTAGGAGCTTTAGAGTGGACAAAAGTTGTGCATAAACATCAGGGATGGAGGCTTGTCACTTGTATTTGGCTGCATGCTGGCATAATCCACCTGGTTGCTAACATGCTCAGCCTCGTCTTCATCGGAATCCGCCTCGAGCAGCAATTCGGGTTTGTGAGAGTTGGAATTGTATACCTATTGTCTGGATTTGGAGGTAGCGTACTTTCTTCGCTTTTTATTCGAAAAAACATCTCGGTTGGTGCCTCCGGCGCTCTCTTCGGGCTTCTTGGAGCAATGCTTTCGGAACTAATTACCAATTGGACAATGTATACCAACAAGGTTGCAGCCCTTCTCACACTTCTGGTCATCGTTGCCATCAACCTCGCCATTGGAATTTTACCACACGTTGATAATTTTGCTCACATTGGCGGTTTTCTGACGGGTTTCCTCATCGGCTTTATCTTGATGCCCCGCCCTCGCCTTGGGTGGTTGGAGAACCCGAATGTTCCTGCCAGAATCAGTGTCGTCAAGTCCAAGTACAAGGCATACCAGTATGTGCTATGGATACTTTCTCTCGTTCTGCTCATCGTCGGATTCACAGTTGCATTGTCGATGCTCTACCGCGGAGAGAACGGAAATGACCGTTGCCGTTGGTGTCACTACCTCAGCTGTGTTCCCACATCTAGATGGCGTTGTGAAGAAAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0002791 GO:0002792 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005794 GO:0005911 GO:0006928 GO:0008150 GO:0008283 GO:0009506 GO:0009894 GO:0009966 GO:0009987 GO:0010646 GO:0012505 GO:0016020 GO:0016477 GO:0019222 GO:0023051 GO:0030054 GO:0030162 GO:0031090 GO:0031323 GO:0031329 GO:0031984 GO:0032268 GO:0032879 GO:0032880 GO:0040011 GO:0042058 GO:0042175 GO:0042176 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0048519 GO:0048523 GO:0048583 GO:0048870 GO:0050708 GO:0050709 GO:0050789 GO:0050794 GO:0051046 GO:0051048 GO:0051049 GO:0051051 GO:0051171 GO:0051179 GO:0051223 GO:0051224 GO:0051246 GO:0051674 GO:0055044 GO:0060255 GO:0061136 GO:0065007 GO:0070201 GO:0080090 GO:0090087 GO:0098588 GO:0098791 GO:0098827 GO:1901184 GO:1903050 GO:1903362 GO:1903530 GO:1903531 GO:1904950

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Rhomboid

Protein Analysis

328

Amino Acids

36.4

Weight (kDa)

9.34

Isoelectric Point (pI)

36.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Rhomboid	PF01694	112 - 249	8.6e-45	Rhomboid domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011656)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G07250 AT5G07250
fragaria_vesca	FvH4_7g29110 FvH4_7g29110
malus_domestica	MD01G1198000.v1.1 MD07G1265600.v1.1
prunus_persica	Prupe.2G291300_v2.0.a1
pyrus_communis	pycom01g20810 pycom07g24380
rosa_chinensis	RchiOBHm_Chr1g0376861
rosa_laevigata	RLG00000026564
rosa_multiflora	Rmu_sc0002113.1_g000004
rosa_roxburghii	Rroxscaffold_4G00281670
rosa_rugosa	Rorug01G0399100
rosa_samantha	Rh1AG416300 Rh1BG375600 Rh1CG389400 Rh1DG406200
rosa_wichuraiana	Rw1G036520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	115, 536
AccI	GTMKAC	2 cut(s)	468, 605
AccII	CGCG	2 cut(s)	77, 900
AciI	CCGC	9 cut(s)	77, 194, 197, 200, 427, 705, 748, 898, 900
AcoI	YGGCCR	1 cut(s)	156
AcsI	RAATTY	1 cut(s)	669
AfaI	GTAC	4 cut(s)	74, 164, 498, 815
AfiI	CCNNNNNNNGG	2 cut(s)	269, 758
AflIII	ACRYGT	1 cut(s)	679
AgsI	TTSAA	1 cut(s)	253
AjnI	CCWGG	1 cut(s)	387
AluBI	AGCT	3 cut(s)	57, 305, 945
AluI	AGCT	3 cut(s)	57, 305, 945
Alw21I	GWGCWC	1 cut(s)	215
Alw44I	GTGCAC	1 cut(s)	211
Ama87I	CYCGRG	1 cut(s)	431
AoxI	GGCC	3 cut(s)	93, 156, 271
ApaLI	GTGCAC	1 cut(s)	211
ApeKI	GCWGC	3 cut(s)	367, 436, 620
ApoI	RAATTY	1 cut(s)	669
AspLEI	GCGC	1 cut(s)	548
AspS9I	GGNCC	2 cut(s)	94, 272
AsuII	TTCGAA	1 cut(s)	517
AvaI	CYCGRG	1 cut(s)	431
BaeGI	GKGCMC	1 cut(s)	215
BalI	TGGCCA	1 cut(s)	158
BanI	GGYRCC	2 cut(s)	115, 536
BbsI	GAAGAC	2 cut(s)	52, 406
Bbv12I	GWGCWC	1 cut(s)	215
BbvCI	CCTCAGC	1 cut(s)	941
BbvI	GCAGC	3 cut(s)	354, 448, 632
BccI	CCATC	3 cut(s)	336, 659, 957
BceAI	ACGGC	3 cut(s)	231, 232, 909
BcgI	CGANNNNNNTGC	2 cut(s)	558, 592
BciT130I	CCWGG	1 cut(s)	389
BciVI	GTATCC	1 cut(s)	834
BfaI	CTAG	1 cut(s)	960
BfmI	CTRYAG	1 cut(s)	6
BfoI	RGCGCY	1 cut(s)	549
BfuI	GTATCC	1 cut(s)	834
BisI	GCNGC	5 cut(s)	195, 198, 368, 437, 621
BlpI	GCTNAGC	1 cut(s)	404
BlsI	GCNGC	5 cut(s)	196, 199, 369, 438, 622
Bme1390I	CCNGG	1 cut(s)	389
BmeT110I	CYCGRG	1 cut(s)	431
BmgT120I	GGNCC	2 cut(s)	94, 272
BmiI	GGNNCC	2 cut(s)	117, 538
BmrFI	CCNGG	1 cut(s)	389
BmsI	GCATC	2 cut(s)	732, 879
BpiI	GAAGAC	2 cut(s)	52, 406
Bpu10I	CCTNAGC	1 cut(s)	941
Bpu1102I	GCTNAGC	1 cut(s)	404
Bpu14I	TTCGAA	1 cut(s)	517
BsaJI	CCNNGG	3 cut(s)	40, 757, 898
BsaXI	ACNNNNNCTCC	4 cut(s)	194, 224, 480, 510
Bsc4I	CCNNNNNNNGG	2 cut(s)	269, 758
Bse1I	ACTGG	1 cut(s)	826
Bse3DI	GCAATG	1 cut(s)	576
BseBI	CCWGG	1 cut(s)	389
BseDI	CCNNGG	3 cut(s)	40, 757, 898
BseGI	GGATG	2 cut(s)	88, 347
BseLI	CCNNNNNNNGG	2 cut(s)	269, 758
BseMI	GCAATG	1 cut(s)	576
BseMII	CTCAG	2 cut(s)	418, 955
BseNI	ACTGG	1 cut(s)	826
BseSI	GKGCMC	1 cut(s)	215
BseXI	GCAGC	3 cut(s)	354, 448, 632
Bsh1236I	CGCG	2 cut(s)	77, 900
BshFI	GGCC	3 cut(s)	95, 158, 273
BshNI	GGYRCC	2 cut(s)	115, 536
BsiHKAI	GWGCWC	1 cut(s)	215
BsiHKCI	CYCGRG	1 cut(s)	431
BsiSI	CCGG	1 cut(s)	543
BsiWI	CGTACG	1 cut(s)	72
BslFI	GGGAC	1 cut(s)	162
BslI	CCNNNNNNNGG	2 cut(s)	269, 758
BsmFI	GGGAC	1 cut(s)	162
BsnI	GGCC	3 cut(s)	95, 158, 273
BsoBI	CYCGRG	1 cut(s)	431
Bsp119I	TTCGAA	1 cut(s)	517
Bsp1286I	GDGCHC	1 cut(s)	215
Bsp1720I	GCTNAGC	1 cut(s)	404
BspACI	CCGC	9 cut(s)	77, 194, 197, 200, 427, 705, 748, 898, 900
BspANI	GGCC	3 cut(s)	95, 158, 273
BspCNI	CTCAG	2 cut(s)	417, 954
BspFNI	CGCG	2 cut(s)	77, 900
BspLI	GGNNCC	2 cut(s)	117, 538
BspMAI	CTGCAG	1 cut(s)	10
BspT104I	TTCGAA	1 cut(s)	517
BspT107I	GGYRCC	2 cut(s)	115, 536
BsrDI	GCAATG	1 cut(s)	576
BsrI	ACTGG	1 cut(s)	826
BssECI	CCNNGG	3 cut(s)	40, 757, 898
BssNAI	GTATAC	2 cut(s)	469, 606
BssT1I	CCWWGG	1 cut(s)	757
Bst1107I	GTATAC	2 cut(s)	469, 606
Bst2UI	CCWGG	1 cut(s)	389
Bst4CI	ACNGT	3 cut(s)	176, 877, 920
Bst6I	CTCTTC	3 cut(s)	84, 272, 557
BstBI	TTCGAA	1 cut(s)	517
BstC8I	GCNNGC	3 cut(s)	6, 372, 376
BstDEI	CTNAG	2 cut(s)	404, 941
BstDSI	CCRYGG	1 cut(s)	898
BstF5I	GGATG	2 cut(s)	88, 347
BstFNI	CGCG	2 cut(s)	77, 900
BstH2I	RGCGCY	1 cut(s)	549
BstHHI	GCGC	1 cut(s)	548
BstMWI	GCNNNNNNNGC	1 cut(s)	545
BstNI	CCWGG	1 cut(s)	389
BstNSI	RCATGY	2 cut(s)	374, 403
BstSCI	CCNGG	1 cut(s)	387
BstSFI	CTRYAG	1 cut(s)	6
BstSLI	GKGCMC	1 cut(s)	215
BstUI	CGCG	2 cut(s)	77, 900
BstV1I	GCAGC	3 cut(s)	354, 448, 632
BstV2I	GAAGAC	2 cut(s)	52, 406
BstZ17I	GTATAC	2 cut(s)	469, 606
BsuI	GTATCC	1 cut(s)	834
BsuRI	GGCC	3 cut(s)	95, 158, 273
BtgI	CCRYGG	1 cut(s)	898
BtsCI	GGATG	2 cut(s)	88, 347
BtsIMutI	CAGTG	1 cut(s)	802
Cac8I	GCNNGC	3 cut(s)	6, 372, 376
CfoI	GCGC	1 cut(s)	548
Cfr13I	GGNCC	2 cut(s)	94, 272
Cfr42I	CCGCGG	1 cut(s)	901
CsiI	ACCWGGT	1 cut(s)	387
Csp6I	GTAC	4 cut(s)	73, 163, 497, 814
CviAII	CATG	3 cut(s)	160, 371, 400
CviQI	GTAC	4 cut(s)	73, 163, 497, 814
DdeI	CTNAG	2 cut(s)	404, 941
EaeI	YGGCCR	1 cut(s)	156
Eam1104I	CTCTTC	3 cut(s)	84, 272, 557
EarI	CTCTTC	3 cut(s)	84, 272, 557
EciI	GGCGGA	2 cut(s)	215, 416
Eco130I	CCWWGG	1 cut(s)	757
Eco88I	CYCGRG	1 cut(s)	431
EcoRII	CCWGG	1 cut(s)	387
EcoT14I	CCWWGG	1 cut(s)	757
ErhI	CCWWGG	1 cut(s)	757
FaeI	CATG	3 cut(s)	163, 374, 403
FaqI	GGGAC	1 cut(s)	162
FatI	CATG	3 cut(s)	159, 370, 399
FauI	CCCGC	1 cut(s)	755
FblI	GTMKAC	2 cut(s)	468, 605
Fnu4HI	GCNGC	5 cut(s)	195, 198, 368, 437, 621
FokI	GGATG	2 cut(s)	95, 354
Fsp4HI	GCNGC	5 cut(s)	195, 198, 368, 437, 621
FspBI	CTAG	1 cut(s)	960
GlaI	GCGC	1 cut(s)	547
GluI	GCNGC	5 cut(s)	195, 198, 368, 437, 621
HaeII	RGCGCY	1 cut(s)	549
HaeIII	GGCC	3 cut(s)	95, 158, 273
HapII	CCGG	1 cut(s)	543
HhaI	GCGC	1 cut(s)	548
Hin1II	CATG	3 cut(s)	163, 374, 403
Hin6I	GCGC	1 cut(s)	546
HinP1I	GCGC	1 cut(s)	546
HinfI	GANTC	3 cut(s)	423, 792, 870
HpaII	CCGG	1 cut(s)	543
Hpy166II	GTNNAC	4 cut(s)	213, 315, 469, 606
Hpy188I	TCNGA	4 cut(s)	422, 580, 714, 869
Hpy188III	TCNNGA	3 cut(s)	480, 739, 960
Hpy8I	GTNNAC	4 cut(s)	213, 315, 469, 606
Hpy99I	CGWCG	2 cut(s)	67, 869
HpyAV	CCTTC	3 cut(s)	247, 285, 635
HpyCH4III	ACNGT	3 cut(s)	176, 877, 920
HpyCH4IV	ACGT	2 cut(s)	165, 681
HpyCH4V	TGCA	6 cut(s)	8, 213, 328, 370, 620, 881
HpyF10VI	GCNNNNNNNGC	1 cut(s)	545
HpyF3I	CTNAG	2 cut(s)	404, 941
HpySE526I	ACGT	2 cut(s)	165, 681
Hsp92II	CATG	3 cut(s)	163, 374, 403
HspAI	GCGC	1 cut(s)	546
KspI	CCGCGG	1 cut(s)	901
LmnI	GCTCC	2 cut(s)	302, 566
Lsp1109I	GCAGC	3 cut(s)	354, 448, 632
LweI	GCATC	2 cut(s)	732, 879
MabI	ACCWGGT	1 cut(s)	387
MaeI	CTAG	1 cut(s)	960
MaeII	ACGT	2 cut(s)	165, 681
MaeIII	GTNAC	2 cut(s)	352, 932
MboII	GAAGA	9 cut(s)	23, 57, 101, 259, 270, 273, 406, 495, 544
MfeI	CAATTG	1 cut(s)	592
MhlI	GDGCHC	1 cut(s)	215
MlsI	TGGCCA	1 cut(s)	158
MluCI	AATT	8 cut(s)	187, 440, 462, 585, 592, 669, 688, 979
MluNI	TGGCCA	1 cut(s)	158
MmeI	TCCRAC	3 cut(s)	439, 747, 847
Mox20I	TGGCCA	1 cut(s)	158
MscI	TGGCCA	1 cut(s)	158
MseI	TTAA	1 cut(s)	982
MslI	CAYNNNNRTG	1 cut(s)	961
Msp20I	TGGCCA	1 cut(s)	158
MspA1I	CMGCKG	2 cut(s)	900, 945
MspI	CCGG	1 cut(s)	543
MspR9I	CCNGG	1 cut(s)	389
MunI	CAATTG	1 cut(s)	592
MvaI	CCWGG	1 cut(s)	389
MvnI	CGCG	2 cut(s)	77, 900
MwoI	GCNNNNNNNGC	1 cut(s)	545
NlaIII	CATG	3 cut(s)	163, 374, 403
NlaIV	GGNNCC	2 cut(s)	117, 538
NmuCI	GTSAC	2 cut(s)	352, 932
NspI	RCATGY	2 cut(s)	374, 403
NspV	TTCGAA	1 cut(s)	517
PaeI	GCATGC	1 cut(s)	374
PaeR7I	CTCGAG	1 cut(s)	431
PcsI	WCGNNNNNNNCGW	2 cut(s)	68, 245
PfeI	GAWTC	3 cut(s)	423, 792, 870
Pfl23II	CGTACG	1 cut(s)	72
PkrI	GCNGC	5 cut(s)	196, 199, 369, 438, 622
Psp6I	CCWGG	1 cut(s)	387
PspGI	CCWGG	1 cut(s)	387
PspLI	CGTACG	1 cut(s)	72
PspN4I	GGNNCC	2 cut(s)	117, 538
PspPI	GGNCC	2 cut(s)	94, 272
PspXI	VCTCGAGB	1 cut(s)	431
PstI	CTGCAG	1 cut(s)	10
PvuII	CAGCTG	1 cut(s)	945
RsaI	GTAC	4 cut(s)	74, 164, 498, 815
RsaNI	GTAC	4 cut(s)	73, 163, 497, 814
RseI	CAYNNNNRTG	1 cut(s)	961
SacII	CCGCGG	1 cut(s)	901
SaqAI	TTAA	1 cut(s)	982
SatI	GCNGC	5 cut(s)	195, 198, 368, 437, 621
Sau96I	GGNCC	2 cut(s)	94, 272
ScrFI	CCNGG	1 cut(s)	389
SduI	GDGCHC	1 cut(s)	215
SexAI	ACCWGGT	1 cut(s)	387
SfaNI	GCATC	2 cut(s)	732, 879
SfcI	CTRYAG	1 cut(s)	6
Sfr274I	CTCGAG	1 cut(s)	431
Sfr303I	CCGCGG	1 cut(s)	901
SfuI	TTCGAA	1 cut(s)	517
SgrBI	CCGCGG	1 cut(s)	901
SlaI	CTCGAG	1 cut(s)	431
SmiMI	CAYNNNNRTG	1 cut(s)	961
SmlI	CTYRAG	1 cut(s)	431
SmoI	CTYRAG	1 cut(s)	431
SphI	GCATGC	1 cut(s)	374
Sse9I	AATT	8 cut(s)	187, 440, 462, 585, 592, 669, 688, 979
SsiI	CCGC	9 cut(s)	77, 194, 197, 200, 427, 705, 748, 898, 900
SspMI	CTAG	1 cut(s)	960
StyD4I	CCNGG	1 cut(s)	387
StyI	CCWWGG	1 cut(s)	757
TaaI	ACNGT	3 cut(s)	176, 877, 920
TaiI	ACGT	2 cut(s)	168, 684
TaqI	TCGA	3 cut(s)	432, 517, 887
TasI	AATT	8 cut(s)	187, 440, 462, 585, 592, 669, 688, 979
TatI	WGTACW	1 cut(s)	813
TauI	GCSGC	2 cut(s)	197, 200
TfiI	GAWTC	3 cut(s)	423, 792, 870
Tru1I	TTAA	1 cut(s)	982
Tru9I	TTAA	1 cut(s)	982
TscAI	CASTG	1 cut(s)	802
TseFI	GTSAC	2 cut(s)	352, 932
TseI	GCWGC	3 cut(s)	367, 436, 620
Tsp45I	GTSAC	2 cut(s)	352, 932
TspDTI	ATGAA	1 cut(s)	406
TspGWI	ACGGA	1 cut(s)	924
TspRI	CASTG	1 cut(s)	802
VneI	GTGCAC	1 cut(s)	211
XapI	RAATTY	1 cut(s)	669
XbaI	TCTAGA	1 cut(s)	959
XceI	RCATGY	2 cut(s)	374, 403
XhoI	CTCGAG	1 cut(s)	431
XmiI	GTMKAC	2 cut(s)	468, 605
XspI	CTAG	1 cut(s)	960

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.