Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0009821

Physical Location & Seq

Genomic Coordinates Reverse (-)

2690879 .. 2693585

Gene Structure 2707 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G08370.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1191 bp

ATGGTTCTTCTTAGTTTCTCCTTAAGATTCATTGCGTTCACTCTGACTATAACTCTGACTCAGATTGCTGATGGGTTTCAGAGTCGAATGTTGATGAACAATGGACTTGCTCTCTCTCCTCAAATGGGATGGAACAGCTGGAATCATTTTCAGTGTAATATCAATGAAACCCTTATCAAACAAACCGCTGATGCAATGGTTTCAAGTGGTCTTTCTGCAATAGGTTACAAGTATATCAACATAGATGATTGTTGGGGTGAACTCAAGAGAGATTCTCAGGGGAGTTTGGTTGCAAAAGCATCAACATTTCCTTCAGGAATCAAAGCTTTATCAGATTATGTTCATAGCAAAGGGCTAAAACTTGGGATATACTCTGATGCCGGGACTCTTACCTGCAGCCAAACCATGCCGGGATCACTCGGGCATGAAGAACAAGATGCCAAGACATTTGCCTCATGGGGGATTGATTACCTCAAGTATGATAACTGCGAAAATACAGGGACAAGTCCAAGAGAAAGATACCCTAAGATGAGTAAAGCGCTGTTAAATTCAGGAAGATCCATATTCTTCTCTCTGTGCGAATGGGGACAAGAGGATCCAGCAACTTGGGCAGGAGATATTGGCAACAGTTGGAGAACAACAGGAGATATCCAAGATAATTGGAAAAGCATGACATTGATAGCGGATCAGAATGATCGATGGGCATCTTATGCAAGACCAGGATCGTGGAACGATCCAGACATGCTTGAAGTGGGAAATGGAGGCATGACTAAGGAAGAATACATGTCGCATTTCAGCATCTGGGCATTGGCAAAAGCTCCTCTGCTGATCGGCTGCGATCTTAGATCGATGGACAAAGTTACATTTGAATTGCTTAGCAACAAAGAGGTGATTGCTGTTAATCAAGACAAACTTGGGATCCAGGGAAAGAAAGTAAAGAAAGAAGGTGATCTTGAGGTATGGGCAGGTCCACTAAGCAAGAAACGAGTAGCGGTCATCCTATGGAACAGAGGATCAGCATCTGCAAACATCACAGCTCGATGGGCTGAGATTGGCCTCAATTCATCAGATATTGTTAATGCTCGTGACTTATGGGAGCATTCGACATATTCATGTGTTAAAAAGCAGCTATCTGCCCTAGTGGAGCCTCATGCCTGCAAAATGTATACTCTTACAAGACGCAAGGCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004557 GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0007275 GO:0008150 GO:0009505 GO:0009605 GO:0009607 GO:0009620 GO:0009653 GO:0009909 GO:0009911 GO:0009965 GO:0010016 GO:0015925 GO:0016787 GO:0016798 GO:0030312 GO:0032501 GO:0032502 GO:0043207 GO:0044464 GO:0048046 GO:0048366 GO:0048367 GO:0048518 GO:0048580 GO:0048582 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050896 GO:0051094 GO:0051239 GO:0051240 GO:0051704 GO:0051707 GO:0065007 GO:0071944 GO:0099402 GO:1905392 GO:2000026 GO:2000241 GO:2000243

KEGG Pathways

Metabolic & Signaling

ko00052 ko00561 ko00600 ko00603 map00052 map00561 map00600 map00603

Pfam Domains

Protein Families

Melibiase_2 Melibiase_2_C

Protein Analysis

396

Amino Acids

44.04

Weight (kDa)

7.54

Isoelectric Point (pI)

40.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Melibiase_2	PF16499	14 - 277	1.5e-79	Alpha galactosidase A
Melibiase_2_C	PF17450	280 - 352	8.4e-07	Alpha galactosidase A C-terminal beta sandwich domain
Melibiase_C	PF17801	289 - 365	2.3e-19	Alpha galactosidase C-terminal beta sandwich domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009821)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G08370 AT5G08370
fragaria_vesca	FvH4_4g05940 FvH4_4g05940 FvH4_4g05940
malus_domestica	MD05G1321500.v1.1 MD05G1321600.v1.1 MD05G1321700.v1.1
prunus_persica	Prupe.4G041000_v2.0.a1 Prupe.4G041000_v2.0.a1
pyrus_communis	pycom05g29600 pycom05g29610
rosa_chinensis	RchiOBHm_Chr4g0397131
rosa_laevigata	RLG00000009444
rosa_multiflora	Rmu_sc0002312.1_g000033
rosa_roxburghii	Rroxscaffold_5G00342100
rosa_rugosa	Rorug03G0344600
rosa_samantha	Rh4AG077500 Rh4CG082700 Rh4DG070600
rosa_wichuraiana	Rw4G006260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	401, 956
AccI	GTMKAC	1 cut(s)	1166
AciI	CCGC	3 cut(s)	186, 683, 992
AcsI	RAATTY	1 cut(s)	547
AcuI	CTGAAG	1 cut(s)	297
AfeI	AGCGCT	1 cut(s)	540
AfiI	CCNNNNNNNGG	2 cut(s)	125, 459
AflII	CTTAAG	1 cut(s)	22
AflIII	ACRYGT	1 cut(s)	783
AgsI	TTSAA	3 cut(s)	204, 749, 869
AjnI	CCWGG	2 cut(s)	718, 921
AluBI	AGCT	5 cut(s)	138, 326, 818, 1037, 1129
AluI	AGCT	5 cut(s)	138, 326, 818, 1037, 1129
AlwNI	CAGNNNCTG	2 cut(s)	801, 1022
Ama87I	CYCGRG	1 cut(s)	419
Aor51HI	AGCGCT	1 cut(s)	540
AoxI	GGCC	1 cut(s)	1054
ApeKI	GCWGC	3 cut(s)	396, 834, 1126
ApoI	RAATTY	1 cut(s)	547
AspLEI	GCGC	1 cut(s)	541
AspS9I	GGNCC	1 cut(s)	968
AsuC2I	CCSGG	2 cut(s)	382, 411
AsuHPI	GGTGA	3 cut(s)	269, 901, 959
AvaI	CYCGRG	1 cut(s)	419
AvaII	GGWCC	1 cut(s)	968
BamHI	GGATCC	2 cut(s)	595, 918
BauI	CACGAG	1 cut(s)	1083
BbvI	GCAGC	3 cut(s)	408, 821, 1138
BccI	CCATC	5 cut(s)	65, 123, 693, 844, 1035
BciT130I	CCWGG	2 cut(s)	720, 923
BcnI	CCSGG	2 cut(s)	382, 411
BfaI	CTAG	1 cut(s)	1139
BfmI	CTRYAG	1 cut(s)	394
BfoI	RGCGCY	1 cut(s)	542
BfrI	CTTAAG	1 cut(s)	22
BfuAI	ACCTGC	2 cut(s)	401, 956
BisI	GCNGC	3 cut(s)	397, 835, 1127
BlpI	GCTNAGC	1 cut(s)	875
BlsI	GCNGC	3 cut(s)	398, 836, 1128
Bme1390I	CCNGG	4 cut(s)	382, 411, 720, 923
Bme18I	GGWCC	1 cut(s)	968
BmeT110I	CYCGRG	1 cut(s)	419
BmgT120I	GGNCC	1 cut(s)	968
BmiI	GGNNCC	3 cut(s)	597, 920, 1146
BmrFI	CCNGG	4 cut(s)	382, 411, 720, 923
BmsI	GCATC	7 cut(s)	181, 308, 367, 427, 713, 807, 1028
BplI	GAGNNNNNCTC	2 cut(s)	259, 291
Bpu1102I	GCTNAGC	1 cut(s)	875
BpuEI	CTTGAG	3 cut(s)	248, 458, 974
BpuMI	CCSGG	2 cut(s)	382, 411
Bsa29I	ATCGAT	2 cut(s)	697, 848
BsaBI	GATNNNNATC	2 cut(s)	703, 1018
BsaJI	CCNNGG	1 cut(s)	922
Bsc4I	CCNNNNNNNGG	2 cut(s)	125, 459
Bse3DI	GCAATG	2 cut(s)	30, 201
Bse8I	GATNNNNATC	2 cut(s)	703, 1018
BseBI	CCWGG	2 cut(s)	720, 923
BseCI	ATCGAT	2 cut(s)	697, 848
BseDI	CCNNGG	1 cut(s)	922
BseGI	GGATG	2 cut(s)	134, 996
BseJI	GATNNNNATC	2 cut(s)	703, 1018
BseLI	CCNNNNNNNGG	2 cut(s)	125, 459
BseMI	GCAATG	2 cut(s)	30, 201
BseMII	CTCAG	3 cut(s)	74, 290, 1038
BseRI	GAGGAG	2 cut(s)	108, 810
BseXI	GCAGC	3 cut(s)	408, 821, 1138
BshFI	GGCC	1 cut(s)	1056
BshVI	ATCGAT	2 cut(s)	697, 848
BsiHKCI	CYCGRG	1 cut(s)	419
BsiSI	CCGG	2 cut(s)	381, 410
BslFI	GGGAC	3 cut(s)	397, 514, 600
BslI	CCNNNNNNNGG	2 cut(s)	125, 459
BsmFI	GGGAC	3 cut(s)	397, 514, 600
BsmI	GAATGC	1 cut(s)	1099
BsnI	GGCC	1 cut(s)	1056
BsoBI	CYCGRG	1 cut(s)	419
Bsp1720I	GCTNAGC	1 cut(s)	875
BspACI	CCGC	3 cut(s)	186, 683, 992
BspANI	GGCC	1 cut(s)	1056
BspCNI	CTCAG	3 cut(s)	73, 289, 1039
BspDI	ATCGAT	2 cut(s)	697, 848
BspLI	GGNNCC	3 cut(s)	597, 920, 1146
BspMAI	CTGCAG	1 cut(s)	398
BspMI	ACCTGC	2 cut(s)	401, 956
BspTI	CTTAAG	1 cut(s)	22
BsrDI	GCAATG	2 cut(s)	30, 201
BssECI	CCNNGG	1 cut(s)	922
BssNAI	GTATAC	1 cut(s)	1167
BssSI	CACGAG	1 cut(s)	1083
Bst1107I	GTATAC	1 cut(s)	1167
Bst2BI	CACGAG	1 cut(s)	1083
Bst2UI	CCWGG	2 cut(s)	720, 923
Bst4CI	ACNGT	1 cut(s)	629
BstAFI	CTTAAG	1 cut(s)	22
BstAPI	GCANNNNNTGC	1 cut(s)	710
BstC8I	GCNNGC	1 cut(s)	1156
BstDEI	CTNAG	9 cut(s)	11, 60, 276, 525, 771, 842, 875, 974, 1047
BstF5I	GGATG	2 cut(s)	134, 996
BstH2I	RGCGCY	1 cut(s)	542
BstHHI	GCGC	1 cut(s)	541
BstMWI	GCNNNNNNNGC	3 cut(s)	608, 710, 1043
BstNI	CCWGG	2 cut(s)	720, 923
BstNSI	RCATGY	2 cut(s)	745, 787
BstSCI	CCNGG	4 cut(s)	380, 409, 718, 921
BstSFI	CTRYAG	1 cut(s)	394
BstV1I	GCAGC	3 cut(s)	408, 821, 1138
BstX2I	RGATCY	3 cut(s)	557, 595, 918
BstXI	CCANNNNNNTGG	2 cut(s)	606, 726
BstYI	RGATCY	3 cut(s)	557, 595, 918
BstZ17I	GTATAC	1 cut(s)	1167
Bsu15I	ATCGAT	2 cut(s)	697, 848
BsuRI	GGCC	1 cut(s)	1056
BsuTUI	ATCGAT	2 cut(s)	697, 848
BtsCI	GGATG	2 cut(s)	134, 996
BtsIMutI	CAGTG	1 cut(s)	158
BveI	ACCTGC	2 cut(s)	401, 956
Cac8I	GCNNGC	1 cut(s)	1156
CaiI	CAGNNNCTG	2 cut(s)	801, 1022
CfoI	GCGC	1 cut(s)	541
Cfr13I	GGNCC	1 cut(s)	968
ClaI	ATCGAT	2 cut(s)	697, 848
CviAII	CATG	9 cut(s)	406, 425, 456, 670, 742, 766, 784, 1113, 1151
DdeI	CTNAG	9 cut(s)	11, 60, 276, 525, 771, 842, 875, 974, 1047
Eco32I	GATATC	1 cut(s)	649
Eco47I	GGWCC	1 cut(s)	968
Eco47III	AGCGCT	1 cut(s)	540
Eco57I	CTGAAG	1 cut(s)	297
Eco88I	CYCGRG	1 cut(s)	419
EcoRII	CCWGG	2 cut(s)	718, 921
EcoRV	GATATC	1 cut(s)	649
FaeI	CATG	9 cut(s)	409, 428, 459, 673, 745, 769, 787, 1116, 1154
FalI	AAGNNNNNCTT	4 cut(s)	897, 929, 936, 968
FaqI	GGGAC	3 cut(s)	397, 514, 600
FatI	CATG	9 cut(s)	405, 424, 455, 669, 741, 765, 783, 1112, 1150
FblI	GTMKAC	1 cut(s)	1166
Fnu4HI	GCNGC	3 cut(s)	397, 835, 1127
FokI	GGATG	2 cut(s)	141, 983
Fsp4HI	GCNGC	3 cut(s)	397, 835, 1127
FspBI	CTAG	1 cut(s)	1139
GlaI	GCGC	1 cut(s)	540
GluI	GCNGC	3 cut(s)	397, 835, 1127
HaeII	RGCGCY	1 cut(s)	542
HaeIII	GGCC	1 cut(s)	1056
HapII	CCGG	2 cut(s)	381, 410
HhaI	GCGC	1 cut(s)	541
Hin1II	CATG	9 cut(s)	409, 428, 459, 673, 745, 769, 787, 1116, 1154
Hin6I	GCGC	1 cut(s)	539
HinP1I	GCGC	1 cut(s)	539
HindIII	AAGCTT	1 cut(s)	324
HinfI	GANTC	7 cut(s)	27, 58, 82, 142, 272, 318, 385
HpaII	CCGG	2 cut(s)	381, 410
HphI	GGTGA	3 cut(s)	269, 901, 959
Hpy166II	GTNNAC	4 cut(s)	39, 260, 971, 1167
Hpy188I	TCNGA	8 cut(s)	45, 57, 63, 81, 334, 376, 690, 1069
Hpy188III	TCNNGA	7 cut(s)	265, 315, 552, 737, 905, 953, 1085
Hpy8I	GTNNAC	4 cut(s)	39, 260, 971, 1167
HpyAV	CCTTC	2 cut(s)	321, 938
HpyCH4III	ACNGT	1 cut(s)	629
HpyCH4V	TGCA	7 cut(s)	194, 218, 293, 396, 713, 1025, 1158
HpyF10VI	GCNNNNNNNGC	3 cut(s)	608, 710, 1043
HpyF3I	CTNAG	9 cut(s)	11, 60, 276, 525, 771, 842, 875, 974, 1047
Hsp92II	CATG	9 cut(s)	409, 428, 459, 673, 745, 769, 787, 1116, 1154
HspAI	GCGC	1 cut(s)	539
LmnI	GCTCC	3 cut(s)	823, 1096, 1144
Lsp1109I	GCAGC	3 cut(s)	408, 821, 1138
LweI	GCATC	7 cut(s)	181, 308, 367, 427, 713, 807, 1028
MaeI	CTAG	1 cut(s)	1139
MaeIII	GTNAC	3 cut(s)	224, 859, 1085
MboII	GAAGA	4 cut(s)	440, 559, 567, 788
MflI	RGATCY	3 cut(s)	557, 595, 918
MluCI	AATT	4 cut(s)	547, 658, 869, 1060
MlyI	GAGTC	3 cut(s)	52, 91, 379
MmeI	TCCRAC	1 cut(s)	611
MseI	TTAA	5 cut(s)	23, 545, 900, 1077, 1119
MslI	CAYNNNNRTG	1 cut(s)	1111
MspA1I	CMGCKG	2 cut(s)	138, 188
MspCI	CTTAAG	1 cut(s)	22
MspI	CCGG	2 cut(s)	381, 410
MspR9I	CCNGG	4 cut(s)	382, 411, 720, 923
Mva1269I	GAATGC	1 cut(s)	1099
MvaI	CCWGG	2 cut(s)	720, 923
MwoI	GCNNNNNNNGC	3 cut(s)	608, 710, 1043
NciI	CCSGG	2 cut(s)	382, 411
NlaIII	CATG	9 cut(s)	409, 428, 459, 673, 745, 769, 787, 1116, 1154
NlaIV	GGNNCC	3 cut(s)	597, 920, 1146
NmuCI	GTSAC	1 cut(s)	1085
NspI	RCATGY	2 cut(s)	745, 787
PciI	ACATGT	1 cut(s)	783
PctI	GAATGC	1 cut(s)	1099
PfeI	GAWTC	4 cut(s)	27, 142, 272, 318
PkrI	GCNGC	3 cut(s)	398, 836, 1128
PleI	GAGTC	3 cut(s)	52, 90, 379
PpsI	GAGTC	3 cut(s)	52, 90, 379
PscI	ACATGT	1 cut(s)	783
Psp6I	CCWGG	2 cut(s)	718, 921
PspGI	CCWGG	2 cut(s)	718, 921
PspN4I	GGNNCC	3 cut(s)	597, 920, 1146
PspPI	GGNCC	1 cut(s)	968
PstI	CTGCAG	1 cut(s)	398
PstNI	CAGNNNCTG	2 cut(s)	801, 1022
PsuI	RGATCY	3 cut(s)	557, 595, 918
PvuII	CAGCTG	1 cut(s)	138
RseI	CAYNNNNRTG	1 cut(s)	1111
SaqAI	TTAA	5 cut(s)	23, 545, 900, 1077, 1119
SatI	GCNGC	3 cut(s)	397, 835, 1127
Sau96I	GGNCC	1 cut(s)	968
SchI	GAGTC	3 cut(s)	52, 91, 379
ScrFI	CCNGG	4 cut(s)	382, 411, 720, 923
SfaNI	GCATC	7 cut(s)	181, 308, 367, 427, 713, 807, 1028
SfcI	CTRYAG	1 cut(s)	394
SinI	GGWCC	1 cut(s)	968
SmiMI	CAYNNNNRTG	1 cut(s)	1111
SmlI	CTYRAG	4 cut(s)	22, 263, 473, 953
SmoI	CTYRAG	4 cut(s)	22, 263, 473, 953
Sse9I	AATT	4 cut(s)	547, 658, 869, 1060
SsiI	CCGC	3 cut(s)	186, 683, 992
SspMI	CTAG	1 cut(s)	1139
StyD4I	CCNGG	4 cut(s)	380, 409, 718, 921
TaaI	ACNGT	1 cut(s)	629
TaqI	TCGA	5 cut(s)	85, 697, 848, 1039, 1103
TasI	AATT	4 cut(s)	547, 658, 869, 1060
TfiI	GAWTC	4 cut(s)	27, 142, 272, 318
Tru1I	TTAA	5 cut(s)	23, 545, 900, 1077, 1119
Tru9I	TTAA	5 cut(s)	23, 545, 900, 1077, 1119
TscAI	CASTG	1 cut(s)	158
TseFI	GTSAC	1 cut(s)	1085
TseI	GCWGC	3 cut(s)	396, 834, 1126
Tsp45I	GTSAC	1 cut(s)	1085
TspDTI	ATGAA	7 cut(s)	19, 110, 180, 332, 441, 1053, 1101
TspRI	CASTG	1 cut(s)	158
Vha464I	CTTAAG	1 cut(s)	22
VpaK11BI	GGWCC	1 cut(s)	968
XapI	RAATTY	1 cut(s)	547
XceI	RCATGY	2 cut(s)	745, 787
XmiI	GTMKAC	1 cut(s)	1166
XspI	CTAG	1 cut(s)	1139

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.