Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4D

Orthogroup ID

OG0009821

Physical Location & Seq

Genomic Coordinates Reverse (-)

10509889 .. 10516254

Gene Structure 6366 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4DG070600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1248 bp

ATGGTCATGGCAGCAGCTCCTTGGTTATGTTCTCGGCAGCAGCGGGCAGCTTTGGTGGCGATCTGCATTTCTTTTGTGTTCAATACCAACTGCGCAGTTGCTGGTCGGCAGATAATAAGATCCAGTACTGGACAGGATCATATTAGGAGGAACCTCTTGGTCAATGGACTAGGTATAACACCACCTATGGGATGGAATAGTTGGAATCACTTCAACTGTCACATTGAAGAAAAATTGATTAGAGAAACTGCGGACTCGATGGTATCAACAGGACTTGCTGCACTTGGCTACGAATATATAAATTTAGATGATTGCTGGGCTGAACTTGATCGAGATTCGGAGGGAAATTTCGTTCCTAAATCTTCAGCATTTCCATCTGGAATCAAGGCTTTAGCAGATTATGTCCACAGCAAAGGGCTGAAGCTTGGGATTTACTCTGATGCTGGAACTCTGACATGCAGTAAACAAATGCCCGGATCACTAGGCCATGAGGAACAAGATGCACAAACTTTCGCTTCCTGGGGAATTGACTACTTGAAGTATGATAATTGTCATAATACGGGTACAAGTCCTAAAGAAAGGTATCCAGTAATGAGTAAAGCTTTACTAAACTCCACAAGGCCCATCTTCTTTTCCCTATGTGAATGGGGACAGGAAGACCCTGCAACTTGGGCAGCGAGTGTTGGAAATAGTTGGAGAACAACAGGAGACATTGGTGATAACTGGAATAGTATGACATCTCGTGCGGATCAAAACGACAAATGGGCATCATACGCTGGGCCTGGAGGATGGAATGATCCTGATATGCTTGAAGTTGGAAATGGAGGTATGACTGAGGAGGAATATCGTGCACATTTCAGCATCTGGGCATTAGCTAAGGCTCCTCTGTTAATTGGATGTGATATACGATCTATGGACAAAGCGACTCTCAATCTGTTAAGCAACAAGGAAGTTATTGCAGTTAATCAAGACAAACTTGGAGAACAAGGGAAACAAGTTACAAGTGGTACGGATTTGCAGGTATGGGCTGGCCCTCTTAGTCATAACAGGGTAGCATTAGTACTGTGGAACAGAGGTCCTTCAAAAGCTACGGTCACTGCTCATTGGTCCGACATAGGCCTTGAACCAACAACCGTTGTCAAACCAAGAGATTTATGGGAGCATTCAACTGGATCACCAGTCAGTGAAACACTCTCTGCCAATCTCGAGTCCCATGCTTGCAAAATGTACGTACTAACTCCCAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004557 GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0007275 GO:0008150 GO:0009505 GO:0009605 GO:0009607 GO:0009620 GO:0009653 GO:0009909 GO:0009911 GO:0009965 GO:0010016 GO:0015925 GO:0016787 GO:0016798 GO:0030312 GO:0032501 GO:0032502 GO:0043207 GO:0044464 GO:0048046 GO:0048366 GO:0048367 GO:0048518 GO:0048580 GO:0048582 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050896 GO:0051094 GO:0051239 GO:0051240 GO:0051704 GO:0051707 GO:0065007 GO:0071944 GO:0099402 GO:1905392 GO:2000026 GO:2000241 GO:2000243

KEGG Pathways

Metabolic & Signaling

ko00052 ko00561 ko00600 ko00603 map00052 map00561 map00600 map00603

Pfam Domains

Protein Families

Melibiase_2 Melibiase_2_C

Protein Analysis

415

Amino Acids

45.57

Weight (kDa)

5.68

Isoelectric Point (pI)

37.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Melibiase_2	PF16499	60 - 324	1.1e-78	Alpha galactosidase A
Melibiase_C	PF17801	337 - 412	1.1e-17	Alpha galactosidase C-terminal beta sandwich domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009821)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G08370 AT5G08370
fragaria_vesca	FvH4_4g05940 FvH4_4g05940 FvH4_4g05940
malus_domestica	MD05G1321500.v1.1 MD05G1321600.v1.1 MD05G1321700.v1.1
prunus_persica	Prupe.4G041000_v2.0.a1 Prupe.4G041000_v2.0.a1
pyrus_communis	pycom05g29600 pycom05g29610
rosa_chinensis	RchiOBHm_Chr4g0397131
rosa_laevigata	RLG00000009444
rosa_multiflora	Rmu_sc0002312.1_g000033
rosa_roxburghii	Rroxscaffold_5G00342100
rosa_rugosa	Rorug03G0344600
rosa_samantha	Rh4AG077500 Rh4CG082700 Rh4DG070600
rosa_wichuraiana	Rw4G006260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	94
Acc36I	ACCTGC	1 cut(s)	1009
AciI	CCGC	3 cut(s)	43, 251, 746
AclWI	GGATC	6 cut(s)	114, 144, 484, 756, 791, 1180
AcsI	RAATTY	2 cut(s)	301, 346
AcuI	CTGAAG	2 cut(s)	348, 440
AfaI	GTAC	6 cut(s)	127, 565, 1009, 1062, 1229, 1233
AfiI	CCNNNNNNNGG	1 cut(s)	188
AgsI	TTSAA	8 cut(s)	82, 214, 227, 538, 812, 1083, 1124, 1167
AjnI	CCWGG	2 cut(s)	518, 781
AloI	GAACNNNNNNTCC	2 cut(s)	336, 368
AluBI	AGCT	6 cut(s)	17, 50, 424, 602, 875, 1088
AluI	AGCT	6 cut(s)	17, 50, 424, 602, 875, 1088
Alw21I	GWGCWC	1 cut(s)	853
Alw26I	GTCTC	1 cut(s)	702
Alw44I	GTGCAC	1 cut(s)	849
AlwI	GGATC	6 cut(s)	114, 144, 484, 756, 791, 1180
AlwNI	CAGNNNCTG	2 cut(s)	101, 864
Ama87I	CYCGRG	1 cut(s)	1205
AoxI	GGCC	5 cut(s)	484, 620, 779, 1030, 1117
ApaLI	GTGCAC	1 cut(s)	849
ApeKI	GCWGC	7 cut(s)	11, 14, 37, 40, 47, 278, 674
ApoI	RAATTY	2 cut(s)	301, 346
Asp700I	GAANNNNTTC	1 cut(s)	209
AspLEI	GCGC	1 cut(s)	95
AspS9I	GGNCC	5 cut(s)	621, 779, 1031, 1076, 1107
AsuC2I	CCSGG	1 cut(s)	474
AsuHPI	GGTGA	2 cut(s)	728, 1167
AvaI	CYCGRG	1 cut(s)	1205
AvaII	GGWCC	2 cut(s)	1076, 1107
BaeGI	GKGCMC	1 cut(s)	853
BauI	CACGAG	1 cut(s)	741
BbsI	GAAGAC	1 cut(s)	663
Bbv12I	GWGCWC	1 cut(s)	853
BbvI	GCAGC	7 cut(s)	23, 26, 49, 52, 59, 265, 686
BccI	CCATC	5 cut(s)	186, 253, 382, 632, 783
BcgI	CGANNNNNNTGC	2 cut(s)	1196, 1230
BciT130I	CCWGG	2 cut(s)	520, 783
BciVI	GTATCC	1 cut(s)	594
BcnI	CCSGG	1 cut(s)	474
BcoDI	GTCTC	1 cut(s)	702
BfaI	CTAG	2 cut(s)	170, 482
BfuAI	ACCTGC	1 cut(s)	1009
BfuI	GTATCC	1 cut(s)	594
BisI	GCNGC	7 cut(s)	12, 15, 38, 41, 48, 279, 675
BlsI	GCNGC	7 cut(s)	13, 16, 39, 42, 49, 280, 676
BmcAI	AGTACT	2 cut(s)	127, 1062
Bme1390I	CCNGG	3 cut(s)	474, 520, 783
Bme18I	GGWCC	2 cut(s)	1076, 1107
BmeT110I	CYCGRG	1 cut(s)	1205
BmgT120I	GGNCC	5 cut(s)	621, 779, 1031, 1076, 1107
BmiI	GGNNCC	2 cut(s)	152, 882
BmrFI	CCNGG	3 cut(s)	474, 520, 783
BmsI	GCATC	4 cut(s)	430, 490, 776, 870
BpiI	GAAGAC	1 cut(s)	663
BpmI	CTGGAG	1 cut(s)	804
Bpu10I	CCTNAGC	1 cut(s)	876
BpuMI	CCSGG	1 cut(s)	474
BsaAI	YACGTR	1 cut(s)	1231
BsaJI	CCNNGG	2 cut(s)	20, 519
Bsc4I	CCNNNNNNNGG	1 cut(s)	188
Bse1I	ACTGG	6 cut(s)	123, 133, 587, 728, 1174, 1178
BseBI	CCWGG	2 cut(s)	520, 783
BseDI	CCNNGG	2 cut(s)	20, 519
BseGI	GGATG	3 cut(s)	197, 794, 902
BseLI	CCNNNNNNNGG	1 cut(s)	188
BseMII	CTCAG	1 cut(s)	825
BseNI	ACTGG	6 cut(s)	123, 133, 587, 728, 1174, 1178
BseRI	GAGGAG	2 cut(s)	851, 873
BseSI	GKGCMC	1 cut(s)	853
BseXI	GCAGC	7 cut(s)	23, 26, 49, 52, 59, 265, 686
BseYI	CCCAGC	2 cut(s)	315, 776
BsgI	GTGCAG	1 cut(s)	264
BshFI	GGCC	5 cut(s)	486, 622, 781, 1032, 1119
BsiHKAI	GWGCWC	1 cut(s)	853
BsiHKCI	CYCGRG	1 cut(s)	1205
BsiSI	CCGG	1 cut(s)	474
BslFI	GGGAC	2 cut(s)	663, 1195
BslI	CCNNNNNNNGG	1 cut(s)	188
BsmAI	GTCTC	1 cut(s)	702
BsmFI	GGGAC	2 cut(s)	663, 1195
BsmI	GAATGC	1 cut(s)	1162
BsnI	GGCC	5 cut(s)	486, 622, 781, 1032, 1119
BsoBI	CYCGRG	1 cut(s)	1205
Bsp1286I	GDGCHC	1 cut(s)	853
Bsp143I	GATC	9 cut(s)	60, 119, 136, 328, 476, 748, 796, 908, 1172
BspACI	CCGC	3 cut(s)	43, 251, 746
BspANI	GGCC	5 cut(s)	486, 622, 781, 1032, 1119
BspCNI	CTCAG	1 cut(s)	826
BspLI	GGNNCC	2 cut(s)	152, 882
BspMI	ACCTGC	1 cut(s)	1009
BspPI	GGATC	6 cut(s)	114, 144, 484, 756, 791, 1180
BsrI	ACTGG	6 cut(s)	123, 133, 587, 728, 1174, 1178
BssECI	CCNNGG	2 cut(s)	20, 519
BssMI	GATC	9 cut(s)	60, 119, 136, 328, 476, 748, 796, 908, 1172
BssSI	CACGAG	1 cut(s)	741
BssT1I	CCWWGG	1 cut(s)	20
Bst2BI	CACGAG	1 cut(s)	741
Bst2UI	CCWGG	2 cut(s)	520, 783
Bst4CI	ACNGT	4 cut(s)	218, 1065, 1093, 1135
BstBAI	YACGTR	1 cut(s)	1231
BstC8I	GCNNGC	3 cut(s)	45, 1030, 1219
BstDEI	CTNAG	3 cut(s)	834, 876, 1037
BstF5I	GGATG	3 cut(s)	197, 794, 902
BstHHI	GCGC	1 cut(s)	95
BstKTI	GATC	9 cut(s)	63, 122, 139, 331, 479, 751, 799, 911, 1175
BstMAI	GTCTC	1 cut(s)	702
BstMBI	GATC	9 cut(s)	60, 119, 136, 328, 476, 748, 796, 908, 1172
BstMWI	GCNNNNNNNGC	3 cut(s)	56, 671, 773
BstNI	CCWGG	2 cut(s)	520, 783
BstNSI	RCATGY	1 cut(s)	459
BstSCI	CCNGG	3 cut(s)	472, 518, 781
BstSLI	GKGCMC	1 cut(s)	853
BstSNI	TACGTA	1 cut(s)	1231
BstV1I	GCAGC	7 cut(s)	23, 26, 49, 52, 59, 265, 686
BstV2I	GAAGAC	1 cut(s)	663
BstX2I	RGATCY	1 cut(s)	119
BstYI	RGATCY	1 cut(s)	119
BsuI	GTATCC	1 cut(s)	594
BsuRI	GGCC	5 cut(s)	486, 622, 781, 1032, 1119
BtsCI	GGATG	3 cut(s)	197, 794, 902
BtsI	GCAGTG	1 cut(s)	1095
BtsIMutI	CAGTG	2 cut(s)	1095, 1189
BveI	ACCTGC	1 cut(s)	1009
Cac8I	GCNNGC	3 cut(s)	45, 1030, 1219
CaiI	CAGNNNCTG	2 cut(s)	101, 864
CfoI	GCGC	1 cut(s)	95
Cfr13I	GGNCC	5 cut(s)	621, 779, 1031, 1076, 1107
Csp6I	GTAC	6 cut(s)	126, 564, 1008, 1061, 1228, 1232
CviAII	CATG	4 cut(s)	7, 456, 488, 1214
CviQI	GTAC	6 cut(s)	126, 564, 1008, 1061, 1228, 1232
DdeI	CTNAG	3 cut(s)	834, 876, 1037
DpnI	GATC	9 cut(s)	62, 121, 138, 330, 478, 750, 798, 910, 1174
DpnII	GATC	9 cut(s)	60, 119, 136, 328, 476, 748, 796, 908, 1172
Eco105I	TACGTA	1 cut(s)	1231
Eco130I	CCWWGG	1 cut(s)	20
Eco147I	AGGCCT	1 cut(s)	1119
Eco47I	GGWCC	2 cut(s)	1076, 1107
Eco57I	CTGAAG	2 cut(s)	348, 440
Eco88I	CYCGRG	1 cut(s)	1205
EcoO109I	RGGNCCY	1 cut(s)	1076
EcoRII	CCWGG	2 cut(s)	518, 781
EcoT14I	CCWWGG	1 cut(s)	20
ErhI	CCWWGG	1 cut(s)	20
FaeI	CATG	4 cut(s)	10, 459, 491, 1217
FalI	AAGNNNNNCTT	2 cut(s)	960, 992
FaqI	GGGAC	2 cut(s)	663, 1195
FatI	CATG	4 cut(s)	6, 455, 487, 1213
FauI	CCCGC	1 cut(s)	36
Fnu4HI	GCNGC	7 cut(s)	12, 15, 38, 41, 48, 279, 675
FokI	GGATG	3 cut(s)	204, 801, 909
Fsp4HI	GCNGC	7 cut(s)	12, 15, 38, 41, 48, 279, 675
FspBI	CTAG	2 cut(s)	170, 482
FspI	TGCGCA	1 cut(s)	94
GlaI	GCGC	1 cut(s)	94
GluI	GCNGC	7 cut(s)	12, 15, 38, 41, 48, 279, 675
GsaI	CCCAGC	2 cut(s)	319, 780
GsuI	CTGGAG	1 cut(s)	804
HaeIII	GGCC	5 cut(s)	486, 622, 781, 1032, 1119
HapII	CCGG	1 cut(s)	474
HhaI	GCGC	1 cut(s)	95
Hin1II	CATG	4 cut(s)	10, 459, 491, 1217
Hin6I	GCGC	1 cut(s)	93
HinP1I	GCGC	1 cut(s)	93
HindIII	AAGCTT	2 cut(s)	422, 600
HinfI	GANTC	6 cut(s)	205, 254, 335, 381, 925, 1208
HpaII	CCGG	1 cut(s)	474
HphI	GGTGA	2 cut(s)	728, 1167
Hpy166II	GTNNAC	3 cut(s)	406, 464, 851
Hpy188I	TCNGA	4 cut(s)	340, 439, 453, 1111
Hpy188III	TCNNGA	5 cut(s)	332, 378, 800, 968, 1205
Hpy8I	GTNNAC	3 cut(s)	406, 464, 851
HpyAV	CCTTC	1 cut(s)	1089
HpyCH4III	ACNGT	4 cut(s)	218, 1065, 1093, 1135
HpyCH4IV	ACGT	1 cut(s)	1230
HpyCH4V	TGCA	9 cut(s)	66, 281, 459, 503, 665, 851, 959, 1018, 1221
HpyF10VI	GCNNNNNNNGC	3 cut(s)	56, 671, 773
HpyF3I	CTNAG	3 cut(s)	834, 876, 1037
HpySE526I	ACGT	1 cut(s)	1230
Hsp92II	CATG	4 cut(s)	10, 459, 491, 1217
HspAI	GCGC	1 cut(s)	93
Kzo9I	GATC	9 cut(s)	60, 119, 136, 328, 476, 748, 796, 908, 1172
LmnI	GCTCC	3 cut(s)	22, 886, 1159
Lsp1109I	GCAGC	7 cut(s)	23, 26, 49, 52, 59, 265, 686
LweI	GCATC	4 cut(s)	430, 490, 776, 870
MaeI	CTAG	2 cut(s)	170, 482
MaeII	ACGT	1 cut(s)	1230
MaeIII	GTNAC	3 cut(s)	218, 997, 1093
MalI	GATC	9 cut(s)	62, 121, 138, 330, 478, 750, 798, 910, 1174
MboI	GATC	9 cut(s)	60, 119, 136, 328, 476, 748, 796, 908, 1172
MboII	GAAGA	4 cut(s)	239, 354, 619, 668
MflI	RGATCY	1 cut(s)	119
MhlI	GDGCHC	1 cut(s)	853
MluCI	AATT	6 cut(s)	233, 301, 346, 525, 547, 891
MlyI	GAGTC	3 cut(s)	248, 919, 1217
MmeI	TCCRAC	5 cut(s)	182, 664, 674, 796, 1134
MroXI	GAANNNNTTC	1 cut(s)	209
MseI	TTAA	3 cut(s)	890, 938, 963
MspA1I	CMGCKG	1 cut(s)	43
MspI	CCGG	1 cut(s)	474
MspR9I	CCNGG	3 cut(s)	474, 520, 783
Mva1269I	GAATGC	1 cut(s)	1162
MvaI	CCWGG	2 cut(s)	520, 783
MwoI	GCNNNNNNNGC	3 cut(s)	56, 671, 773
NciI	CCSGG	1 cut(s)	474
NdeII	GATC	9 cut(s)	60, 119, 136, 328, 476, 748, 796, 908, 1172
NlaIII	CATG	4 cut(s)	10, 459, 491, 1217
NlaIV	GGNNCC	2 cut(s)	152, 882
NmeAIII	GCCGAG	1 cut(s)	13
NmuCI	GTSAC	2 cut(s)	218, 1093
NsbI	TGCGCA	1 cut(s)	94
NspI	RCATGY	1 cut(s)	459
PaeR7I	CTCGAG	1 cut(s)	1205
PceI	AGGCCT	1 cut(s)	1119
PctI	GAATGC	1 cut(s)	1162
PdmI	GAANNNNTTC	1 cut(s)	209
PfeI	GAWTC	3 cut(s)	205, 335, 381
PkrI	GCNGC	7 cut(s)	13, 16, 39, 42, 49, 280, 676
PleI	GAGTC	3 cut(s)	248, 919, 1216
PpsI	GAGTC	3 cut(s)	248, 919, 1216
Ppu21I	YACGTR	1 cut(s)	1231
PpuMI	RGGWCCY	1 cut(s)	1076
Psp5II	RGGWCCY	1 cut(s)	1076
Psp6I	CCWGG	2 cut(s)	518, 781
PspFI	CCCAGC	2 cut(s)	315, 776
PspGI	CCWGG	2 cut(s)	518, 781
PspN4I	GGNNCC	2 cut(s)	152, 882
PspPI	GGNCC	5 cut(s)	621, 779, 1031, 1076, 1107
PspPPI	RGGWCCY	1 cut(s)	1076
PstNI	CAGNNNCTG	2 cut(s)	101, 864
PsuI	RGATCY	1 cut(s)	119
RsaI	GTAC	6 cut(s)	127, 565, 1009, 1062, 1229, 1233
RsaNI	GTAC	6 cut(s)	126, 564, 1008, 1061, 1228, 1232
SaqAI	TTAA	3 cut(s)	890, 938, 963
SatI	GCNGC	7 cut(s)	12, 15, 38, 41, 48, 279, 675
Sau3AI	GATC	9 cut(s)	60, 119, 136, 328, 476, 748, 796, 908, 1172
Sau96I	GGNCC	5 cut(s)	621, 779, 1031, 1076, 1107
ScaI	AGTACT	2 cut(s)	127, 1062
SchI	GAGTC	3 cut(s)	248, 919, 1217
ScrFI	CCNGG	3 cut(s)	474, 520, 783
SduI	GDGCHC	1 cut(s)	853
SfaNI	GCATC	4 cut(s)	430, 490, 776, 870
Sfr274I	CTCGAG	1 cut(s)	1205
SinI	GGWCC	2 cut(s)	1076, 1107
SlaI	CTCGAG	1 cut(s)	1205
SmlI	CTYRAG	1 cut(s)	1205
SmoI	CTYRAG	1 cut(s)	1205
SnaBI	TACGTA	1 cut(s)	1231
Sse9I	AATT	6 cut(s)	233, 301, 346, 525, 547, 891
SseBI	AGGCCT	1 cut(s)	1119
SsiI	CCGC	3 cut(s)	43, 251, 746
SspMI	CTAG	2 cut(s)	170, 482
StuI	AGGCCT	1 cut(s)	1119
StyD4I	CCNGG	3 cut(s)	472, 518, 781
StyI	CCWWGG	1 cut(s)	20
TaaI	ACNGT	4 cut(s)	218, 1065, 1093, 1135
TaiI	ACGT	1 cut(s)	1233
TaqI	TCGA	3 cut(s)	257, 331, 1206
TasI	AATT	6 cut(s)	233, 301, 346, 525, 547, 891
TatI	WGTACW	2 cut(s)	125, 1060
TfiI	GAWTC	3 cut(s)	205, 335, 381
Tru1I	TTAA	3 cut(s)	890, 938, 963
Tru9I	TTAA	3 cut(s)	890, 938, 963
TscAI	CASTG	2 cut(s)	1102, 1189
TseFI	GTSAC	2 cut(s)	218, 1093
TseI	GCWGC	7 cut(s)	11, 14, 37, 40, 47, 278, 674
Tsp45I	GTSAC	2 cut(s)	218, 1093
TspGWI	ACGGA	1 cut(s)	1025
TspRI	CASTG	2 cut(s)	1102, 1189
VneI	GTGCAC	1 cut(s)	849
VpaK11BI	GGWCC	2 cut(s)	1076, 1107
XapI	RAATTY	2 cut(s)	301, 346
XceI	RCATGY	1 cut(s)	459
XcmI	CCANNNNNNNNNTGG	2 cut(s)	189, 1152
XhoI	CTCGAG	1 cut(s)	1205
XmnI	GAANNNNTTC	1 cut(s)	209
XspI	CTAG	2 cut(s)	170, 482
ZrmI	AGTACT	2 cut(s)	127, 1062

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.