pycom05g29600

Alpha galactosidase A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0009821
Physical Location & Seq
Forward (+)
30005431 .. 30008326
2896 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g29600.8

Sequence Viewer

Length: 1392 bp
ATGCTTATTATAATGTTGTTTCTCACTGAGAATCTAGTAGGTTGTGTCGGTACTGGCACCCCCCCAAACCCCATTTTCTCATTAAAACTTCCGCCACACTCAAAATTCCCTCATCACTCCTACTCTTCCCAAACTCATTTCTTGATCCTCTTCAGAGCTCTTGGATCAGACACCACAATGGATTGGAAACGCAGGAGCGCACTCTTTGTTCAGCTCATTGTTGTCACATGTATTTTCAATGTGATTGATGCTCTGAACTTCAAGAACAATACCCATCAAGCAGAATACGGTCAGTTCCTCATTGCCAATGGCGTTGCTCGTACACCTCCAATGGGTTGGAACAGTTGGAATCACTTCCACTGCAATATAAGTGAAGCCACAGTGAAGACCATTGCGGATGCTCTTGTTTCGACTGGCTTGGCAGCACTTGGATACAACTATGTCAATCTAGATGATTGTTGGGCTGAAAGAAACAGAGACAGTAGTGGTAACCTAATAGCAAAATCGTCAACTTTTCCTTCTGGAATCAAGGCCCTTGCTGATTATGTTCACGCAAGGGGTTTACAACTCGGCATATACTCTGATGCTGGTTATTATACTTGCAGCAAGAAAATGCCAGGCTCACTTGGGCACGAAGAGCAAGACGCTAGAACCTTTGCTGAATGGGGTGTCGATTATTTGAAGTACGATAACTGCAACCATGATGGTTCTAAACCTCAAATCAGATATCCTATAATGAGTTCTGCATTGCTAAAGACTGGAAGGCCAATCCTGTACTCCATATGCGAATGGGGACAAGAGGATCCTGCGAAATGGGCTGGTCGGTATGGCAATGCTTGGAGAACTACGGGAGACATTCAGGACACTTGGGAAAGTATCACGTCGATTGCGGACCAAAACAACATTTGGGCTACATATGCAGGCCCTGGCAAGTGGAATGATCCTGACATGCTGGAAGTGGGAAACGGAGGGATGAGTGTAGAGGAGTACCAATCTCATTTTAGCATTTGGGCAGTCATGAAAGCTCCTTTACTTATCGGATGTGACATCCCATCTGCGAGCAAGGAAACTCTTCAGATTCTCGGAAACAAAGAGGTTATTGATGTTAATCAGGATTCACTCGGAGTTCAAGCGAGGAAACTGCGATCCAAAGATGACCTAGAAGTGTGGGCAGGACCGTTATCAAGGAAAAGAGTCGTGGTAGTGTTGTGGAATAGAGGCGGGTCATTAGCTCCTATAACTGTGACATGGAGGGAAATTGGACTCTCGCCAGATACACCTGTTATTGTTAGAGACTTATGGGCGCACTCATTTGTTTCGAGGAACATGCATACTAGTCTGACTGCAAATGTTGCTTCTCATGGTTGTAATATGTATGTCCTGACTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 ko00561 ko00600 ko00603 map00052 map00561 map00600 map00603
Pfam Domains
Protein Families
Melibiase_2 Melibiase_2_C

Protein Analysis

464

Amino Acids

51.42

Weight (kDa)

6.53

Isoelectric Point (pI)

32.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 11
AccB1I GGYRCC 1 cut(s) 56
AciI CCGC 4 cut(s) 92, 395, 890, 1221
AclWI GGATC 6 cut(s) 139, 172, 797, 810, 935, 1140
AcsI RAATTY 1 cut(s) 104
AcuI CTGAAG 2 cut(s) 136, 1058
AfaI GTAC 5 cut(s) 52, 322, 686, 776, 989
AfiI CCNNNNNNNGG 1 cut(s) 332
AflIII ACRYGT 1 cut(s) 227
AgsI TTSAA 4 cut(s) 238, 262, 682, 1130
AhlI ACTAGT 1 cut(s) 1334
AjiI CACGTC 1 cut(s) 882
AjnI CCWGG 2 cut(s) 616, 925
AluBI AGCT 4 cut(s) 158, 214, 1025, 1232
AluI AGCT 4 cut(s) 158, 214, 1025, 1232
Alw21I GWGCWC 1 cut(s) 160
Alw26I GTCTC 3 cut(s) 471, 846, 1287
AlwI GGATC 6 cut(s) 139, 172, 797, 810, 935, 1140
AlwNI CAGNNNCTG 1 cut(s) 926
AoxI GGCC 3 cut(s) 531, 764, 922
ApeKI GCWGC 2 cut(s) 422, 603
ApoI RAATTY 1 cut(s) 104
Asp700I GAANNNNTTC 2 cut(s) 353, 1071
AspLEI GCGC 2 cut(s) 200, 1306
AspS9I GGNCC 4 cut(s) 532, 892, 923, 1175
AvaII GGWCC 2 cut(s) 892, 1175
BaeGI GKGCMC 1 cut(s) 633
BaeI ACNNNNGTAYC 2 cut(s) 1266, 1299
BamHI GGATCC 1 cut(s) 802
BanI GGYRCC 1 cut(s) 56
BanII GRGCYC 1 cut(s) 160
BbsI GAAGAC 1 cut(s) 392
Bbv12I GWGCWC 1 cut(s) 160
BbvI GCAGC 2 cut(s) 434, 615
BccI CCATC 3 cut(s) 282, 698, 1060
BcgI CGANNNNNNTGC 4 cut(s) 1123, 1157, 1309, 1343
BciT130I CCWGG 2 cut(s) 618, 927
BciVI GTATCC 1 cut(s) 425
BcoDI GTCTC 3 cut(s) 471, 846, 1287
BcuI ACTAGT 1 cut(s) 1334
BfaI CTAG 5 cut(s) 35, 449, 648, 1160, 1335
BfuI GTATCC 1 cut(s) 425
BisI GCNGC 2 cut(s) 423, 604
BlsI GCNGC 2 cut(s) 424, 605
Bme1390I CCNGG 2 cut(s) 618, 927
Bme18I GGWCC 2 cut(s) 892, 1175
BmgBI CACGTC 1 cut(s) 882
BmgT120I GGNCC 4 cut(s) 532, 892, 923, 1175
BmiI GGNNCC 2 cut(s) 58, 804
BmrFI CCNGG 2 cut(s) 618, 927
BmsI GCATC 3 cut(s) 238, 388, 574
BpiI GAAGAC 1 cut(s) 392
BsaBI GATNNNNATC 1 cut(s) 1107
BsaJI CCNNGG 1 cut(s) 925
Bsc4I CCNNNNNNNGG 1 cut(s) 332
Bse1I ACTGG 3 cut(s) 58, 418, 763
Bse3DI GCAATG 4 cut(s) 300, 390, 746, 838
Bse8I GATNNNNATC 1 cut(s) 1107
BseBI CCWGG 2 cut(s) 618, 927
BseDI CCNNGG 1 cut(s) 925
BseGI GGATG 4 cut(s) 403, 978, 1046, 1047
BseJI GATNNNNATC 1 cut(s) 1107
BseLI CCNNNNNNNGG 1 cut(s) 332
BseMI GCAATG 4 cut(s) 300, 390, 746, 838
BseMII CTCAG 1 cut(s) 18
BseNI ACTGG 3 cut(s) 58, 418, 763
BseRI GAGGAG 1 cut(s) 998
BseSI GKGCMC 1 cut(s) 633
BseXI GCAGC 2 cut(s) 434, 615
BshFI GGCC 3 cut(s) 533, 766, 924
BshNI GGYRCC 1 cut(s) 56
BsiHKAI GWGCWC 1 cut(s) 160
BslFI GGGAC 1 cut(s) 807
BslI CCNNNNNNNGG 1 cut(s) 332
BsmAI GTCTC 3 cut(s) 471, 846, 1287
BsmFI GGGAC 1 cut(s) 807
BsnI GGCC 3 cut(s) 533, 766, 924
Bsp1286I GDGCHC 2 cut(s) 160, 633
Bsp143I GATC 5 cut(s) 144, 164, 802, 940, 1145
BspACI CCGC 4 cut(s) 92, 395, 890, 1221
BspANI GGCC 3 cut(s) 533, 766, 924
BspCNI CTCAG 1 cut(s) 19
BspHI TCATGA 1 cut(s) 1017
BspLI GGNNCC 2 cut(s) 58, 804
BspPI GGATC 6 cut(s) 139, 172, 797, 810, 935, 1140
BspQI GCTCTTC 1 cut(s) 630
BspT107I GGYRCC 1 cut(s) 56
BsrDI GCAATG 4 cut(s) 300, 390, 746, 838
BsrI ACTGG 3 cut(s) 58, 418, 763
BssECI CCNNGG 1 cut(s) 925
BssMI GATC 5 cut(s) 144, 164, 802, 940, 1145
Bst2UI CCWGG 2 cut(s) 618, 927
Bst4CI ACNGT 6 cut(s) 290, 344, 382, 482, 1179, 1243
Bst6I CTCTTC 4 cut(s) 130, 155, 630, 1077
BstAPI GCANNNNNTGC 1 cut(s) 1352
BstC8I GCNNGC 2 cut(s) 922, 1060
BstDEI CTNAG 1 cut(s) 27
BstEII GGTNACC 1 cut(s) 488
BstF5I GGATG 4 cut(s) 403, 978, 1046, 1047
BstHHI GCGC 2 cut(s) 200, 1306
BstKTI GATC 5 cut(s) 147, 167, 805, 943, 1148
BstMAI GTCTC 3 cut(s) 471, 846, 1287
BstMBI GATC 5 cut(s) 144, 164, 802, 940, 1145
BstMWI GCNNNNNNNGC 4 cut(s) 637, 815, 917, 1352
BstNI CCWGG 2 cut(s) 618, 927
BstNSI RCATGY 3 cut(s) 231, 952, 1330
BstPI GGTNACC 1 cut(s) 488
BstSCI CCNGG 2 cut(s) 616, 925
BstSLI GKGCMC 1 cut(s) 633
BstV1I GCAGC 2 cut(s) 434, 615
BstV2I GAAGAC 1 cut(s) 392
BstX2I RGATCY 1 cut(s) 802
BstXI CCANNNNNNTGG 1 cut(s) 336
BstYI RGATCY 1 cut(s) 802
BsuI GTATCC 1 cut(s) 425
BsuRI GGCC 3 cut(s) 533, 766, 924
BtrI CACGTC 1 cut(s) 882
BtsCI GGATG 4 cut(s) 403, 978, 1046, 1047
BtsI GCAGTG 1 cut(s) 358
BtsIMutI CAGTG 3 cut(s) 24, 358, 387
Cac8I GCNNGC 2 cut(s) 922, 1060
CaiI CAGNNNCTG 1 cut(s) 926
CciI TCATGA 1 cut(s) 1017
CfoI GCGC 2 cut(s) 200, 1306
Cfr13I GGNCC 4 cut(s) 532, 892, 923, 1175
CseI GACGC 1 cut(s) 653
Csp6I GTAC 5 cut(s) 51, 321, 685, 775, 988
CviAII CATG 7 cut(s) 228, 701, 949, 1018, 1248, 1327, 1361
CviQI GTAC 5 cut(s) 51, 321, 685, 775, 988
DdeI CTNAG 1 cut(s) 27
DpnI GATC 5 cut(s) 146, 166, 804, 942, 1147
DpnII GATC 5 cut(s) 144, 164, 802, 940, 1145
Eam1104I CTCTTC 4 cut(s) 130, 155, 630, 1077
EarI CTCTTC 4 cut(s) 130, 155, 630, 1077
EciI GGCGGA 1 cut(s) 81
Ecl136II GAGCTC 1 cut(s) 158
Eco24I GRGCYC 1 cut(s) 160
Eco32I GATATC 1 cut(s) 728
Eco47I GGWCC 2 cut(s) 892, 1175
Eco53kI GAGCTC 1 cut(s) 158
Eco57I CTGAAG 2 cut(s) 136, 1058
Eco91I GGTNACC 1 cut(s) 488
EcoICRI GAGCTC 1 cut(s) 158
EcoO109I RGGNCCY 2 cut(s) 532, 923
EcoO65I GGTNACC 1 cut(s) 488
EcoRII CCWGG 2 cut(s) 616, 925
EcoRV GATATC 1 cut(s) 728
EcoT22I ATGCAT 1 cut(s) 1332
EcoT38I GRGCYC 1 cut(s) 160
FaeI CATG 7 cut(s) 231, 704, 952, 1021, 1251, 1330, 1364
FaqI GGGAC 1 cut(s) 807
FatI CATG 7 cut(s) 227, 700, 948, 1017, 1247, 1326, 1360
FauI CCCGC 1 cut(s) 1214
FauNDI CATATG 2 cut(s) 782, 916
Fnu4HI GCNGC 2 cut(s) 423, 604
FokI GGATG 4 cut(s) 410, 985, 1034, 1053
FriOI GRGCYC 1 cut(s) 160
Fsp4HI GCNGC 2 cut(s) 423, 604
FspBI CTAG 5 cut(s) 35, 449, 648, 1160, 1335
GlaI GCGC 2 cut(s) 199, 1305
GluI GCNGC 2 cut(s) 423, 604
HaeIII GGCC 3 cut(s) 533, 766, 924
HgaI GACGC 1 cut(s) 653
HhaI GCGC 2 cut(s) 200, 1306
Hin1II CATG 7 cut(s) 231, 704, 952, 1021, 1251, 1330, 1364
Hin6I GCGC 2 cut(s) 198, 1304
HinP1I GCGC 2 cut(s) 198, 1304
HincII GTYRAC 1 cut(s) 510
HindII GTYRAC 1 cut(s) 510
HinfI GANTC 8 cut(s) 31, 349, 525, 1078, 1115, 1194, 1263, 1384
Hpy166II GTNNAC 4 cut(s) 323, 510, 550, 563
Hpy188III TCNNGA 9 cut(s) 142, 262, 449, 522, 860, 944, 1018, 1112, 1381
Hpy8I GTNNAC 4 cut(s) 323, 510, 550, 563
Hpy99I CGWCG 1 cut(s) 886
HpyAV CCTTC 2 cut(s) 528, 756
HpyCH4III ACNGT 6 cut(s) 290, 344, 382, 482, 1179, 1243
HpyCH4IV ACGT 1 cut(s) 881
HpyCH4V TGCA 7 cut(s) 363, 603, 696, 746, 920, 1330, 1346
HpyF10VI GCNNNNNNNGC 4 cut(s) 637, 815, 917, 1352
HpyF3I CTNAG 1 cut(s) 27
HpySE526I ACGT 1 cut(s) 881
Hsp92II CATG 7 cut(s) 231, 704, 952, 1021, 1251, 1330, 1364
HspAI GCGC 2 cut(s) 198, 1304
Kzo9I GATC 5 cut(s) 144, 164, 802, 940, 1145
LguI GCTCTTC 1 cut(s) 630
LmnI GCTCC 3 cut(s) 195, 1030, 1237
Lsp1109I GCAGC 2 cut(s) 434, 615
LweI GCATC 3 cut(s) 238, 388, 574
MaeI CTAG 5 cut(s) 35, 449, 648, 1160, 1335
MaeII ACGT 1 cut(s) 881
MaeIII GTNAC 4 cut(s) 223, 488, 1043, 1243
MalI GATC 5 cut(s) 146, 166, 804, 942, 1147
MboI GATC 5 cut(s) 144, 164, 802, 940, 1145
MboII GAAGA 5 cut(s) 117, 142, 397, 647, 1064
MflI RGATCY 1 cut(s) 802
MhlI GDGCHC 2 cut(s) 160, 633
MluCI AATT 2 cut(s) 104, 1257
MlyI GAGTC 3 cut(s) 1203, 1257, 1378
MmeI TCCRAC 2 cut(s) 317, 326
Mph1103I ATGCAT 1 cut(s) 1332
MroXI GAANNNNTTC 2 cut(s) 353, 1071
MseI TTAA 3 cut(s) 83, 1107, 1390
MslI CAYNNNNRTG 1 cut(s) 176
MspR9I CCNGG 2 cut(s) 618, 927
MvaI CCWGG 2 cut(s) 618, 927
MwoI GCNNNNNNNGC 4 cut(s) 637, 815, 917, 1352
NdeI CATATG 2 cut(s) 782, 916
NdeII GATC 5 cut(s) 144, 164, 802, 940, 1145
NlaIII CATG 7 cut(s) 231, 704, 952, 1021, 1251, 1330, 1364
NlaIV GGNNCC 2 cut(s) 58, 804
NmeAIII GCCGAG 1 cut(s) 549
NmuCI GTSAC 3 cut(s) 223, 1043, 1243
NsiI ATGCAT 1 cut(s) 1332
NspI RCATGY 3 cut(s) 231, 952, 1330
PagI TCATGA 1 cut(s) 1017
PciI ACATGT 1 cut(s) 227
PciSI GCTCTTC 1 cut(s) 630
PdmI GAANNNNTTC 2 cut(s) 353, 1071
PfeI GAWTC 5 cut(s) 31, 349, 525, 1078, 1115
PkrI GCNGC 2 cut(s) 424, 605
PleI GAGTC 3 cut(s) 1202, 1257, 1378
PpsI GAGTC 3 cut(s) 1202, 1257, 1378
PscI ACATGT 1 cut(s) 227
PsiI TTATAA 1 cut(s) 11
Psp124BI GAGCTC 1 cut(s) 160
Psp6I CCWGG 2 cut(s) 616, 925
PspEI GGTNACC 1 cut(s) 488
PspGI CCWGG 2 cut(s) 616, 925
PspN4I GGNNCC 2 cut(s) 58, 804
PspPI GGNCC 4 cut(s) 532, 892, 923, 1175
PstNI CAGNNNCTG 1 cut(s) 926
PsuI RGATCY 1 cut(s) 802
RsaI GTAC 5 cut(s) 52, 322, 686, 776, 989
RsaNI GTAC 5 cut(s) 51, 321, 685, 775, 988
RseI CAYNNNNRTG 1 cut(s) 176
SacI GAGCTC 1 cut(s) 160
SapI GCTCTTC 1 cut(s) 630
SaqAI TTAA 3 cut(s) 83, 1107, 1390
SatI GCNGC 2 cut(s) 423, 604
Sau3AI GATC 5 cut(s) 144, 164, 802, 940, 1145
Sau96I GGNCC 4 cut(s) 532, 892, 923, 1175
SchI GAGTC 3 cut(s) 1203, 1257, 1378
ScrFI CCNGG 2 cut(s) 618, 927
SduI GDGCHC 2 cut(s) 160, 633
SfaNI GCATC 3 cut(s) 238, 388, 574
SinI GGWCC 2 cut(s) 892, 1175
SmiMI CAYNNNNRTG 1 cut(s) 176
SpeI ACTAGT 1 cut(s) 1334
Sse9I AATT 2 cut(s) 104, 1257
SsiI CCGC 4 cut(s) 92, 395, 890, 1221
SspMI CTAG 5 cut(s) 35, 449, 648, 1160, 1335
SstI GAGCTC 1 cut(s) 160
StyD4I CCNGG 2 cut(s) 616, 925
TaaI ACNGT 6 cut(s) 290, 344, 382, 482, 1179, 1243
TaiI ACGT 1 cut(s) 884
TaqI TCGA 4 cut(s) 410, 672, 884, 1319
TasI AATT 2 cut(s) 104, 1257
TatI WGTACW 1 cut(s) 774
TfiI GAWTC 5 cut(s) 31, 349, 525, 1078, 1115
Tru1I TTAA 3 cut(s) 83, 1107, 1390
Tru9I TTAA 3 cut(s) 83, 1107, 1390
TscAI CASTG 3 cut(s) 31, 365, 387
TseFI GTSAC 3 cut(s) 223, 1043, 1243
TseI GCWGC 2 cut(s) 422, 603
Tsp45I GTSAC 3 cut(s) 223, 1043, 1243
TspDTI ATGAA 1 cut(s) 1034
TspGWI ACGGA 1 cut(s) 981
TspRI CASTG 3 cut(s) 31, 365, 387
VpaK11BI GGWCC 2 cut(s) 892, 1175
XapI RAATTY 1 cut(s) 104
XbaI TCTAGA 1 cut(s) 448
XceI RCATGY 3 cut(s) 231, 952, 1330
XmnI GAANNNNTTC 2 cut(s) 353, 1071
XspI CTAG 5 cut(s) 35, 449, 648, 1160, 1335
Zsp2I ATGCAT 1 cut(s) 1332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.