MD05G1321700.v1.1

Alpha galactosidase A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0009821
Physical Location & Seq
Forward (+)
44925220 .. 44928225
3006 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1321700.v1.1.491

Sequence Viewer

Length: 1215 bp
ATGGATTGGAAACGCAGGAGCTCACTCTTTGTTCTGCTCTTTGTTATCTCCTCTATTTTCAATGTGATCGGTGCTCTGAACTTGAAGAATAATACCCACCAAGCAGAATACGGTCAGTTCCTCCTTGCCAATGGCGTTGCTCGTACACCTCCAATGGGTTGGAACAGTTGGAATCACTTCCAATGCAATATAAATGAAGCCACAGTGAAGACCACTGCGGATGCTCTTGTTTCGACTGGCTTGGCAGCACTTGGATACAAATATGTCAATATAGATGATTGTTGGGCTGAAAGAAACAGAGACAGTAGTGGTAACCTAATAGCAAAATCGTCAACTTTTCCATCTGGAATCAAGGCCCTTGCTGATTATGTTCACGCAAGGGGTTTACAGCTAGGCATATACTCTGATGCTGGTCATTATACTTGCAGCAACACAATGCCAGGCTCACTTGGGCACGAAGAGCAAGACGCTAGAACCTTTGCTGAATGGGGTGTCGATTATTTGAAGTACGATAACTGCTACAATGATGGTTCTAAACCTCAAATCAGATATCCTATAATGAGTTCTGCATTGCTAAAGGCTGGAAGGCCAATCCTGTACTCCATATGCGAATGGGGACAAGAGGATCCTGCGAAATGGGCTGGTCAGTATGGCAATGTTTGGAGAACCACGGGAGACATTAAGGACACTTGGGAAAGTATCACGTCGATTGCAGATCAAAACAACATTTGGGCTACATATGCAGGTCCTGGCAAGTGGAACGATCCTGACATGTTGGAAGTGGGAAATGGAGGGATGAGTGTACAGGAGTACCAATCTCATTTTAGCATTTGGGCAGTCATGAAAGCTCCTTTACTTATCGGATGTGACATCCCATCAGCAAGCAAGGAAACTCTTCAGATTCTCGGAAATAAAGAGGTTATTGATGTTAATCAGGATCCACTCGGAGTTCAAGCGAGGAAACTGCGATCCAAAGATGACCTAGAAGTGTGGGCAGGACCATTATCAAATAAAAGAGTGGTGGTAGTGTTGTGGAATAGAGGCCGGTCATTAGCTCCTATAACTGTGACATGGAAGGAAGTTGGACTCTCGCCAAATACACCTGTTACAGTCAGAGACTTATGGGCGCACTCATTTGTTTCAAGGCGCATGCTCACTGGGCTGACTGCAAATGTTGCTCCTCATGGTTGTAAGATGTATGTCCTGACTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 ko00561 ko00600 ko00603 map00052 map00561 map00600 map00603
Pfam Domains
Protein Families
Melibiase_2 Melibiase_2_C

Protein Analysis

405

Amino Acids

44.81

Weight (kDa)

7.07

Isoelectric Point (pI)

36.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Melibiase PF02065 28 - 135 1.9e-06 Melibiase
Melibiase_2 PF16499 49 - 313 2.8e-81 Alpha galactosidase A
Melibiase_2_C PF17450 316 - 394 9.4e-06 Alpha galactosidase A C-terminal beta sandwich domain
Melibiase_C PF17801 326 - 402 1.3e-20 Alpha galactosidase C-terminal beta sandwich domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 734
AciI CCGC 1 cut(s) 218
AclWI GGATC 6 cut(s) 620, 633, 758, 932, 945, 963
AcuI CTGAAG 1 cut(s) 881
AfaI GTAC 5 cut(s) 145, 509, 599, 804, 812
AfiI CCNNNNNNNGG 1 cut(s) 155
AflIII ACRYGT 1 cut(s) 771
AgsI TTSAA 5 cut(s) 61, 85, 505, 953, 1143
AjiI CACGTC 1 cut(s) 705
AjnI CCWGG 2 cut(s) 439, 748
AluBI AGCT 4 cut(s) 21, 391, 848, 1055
AluI AGCT 4 cut(s) 21, 391, 848, 1055
Alw21I GWGCWC 2 cut(s) 23, 76
Alw26I GTCTC 3 cut(s) 294, 669, 1110
AlwI GGATC 6 cut(s) 620, 633, 758, 932, 945, 963
AlwNI CAGNNNCTG 1 cut(s) 749
AoxI GGCC 3 cut(s) 354, 587, 1042
ApeKI GCWGC 2 cut(s) 245, 426
Asp700I GAANNNNTTC 2 cut(s) 176, 894
AspLEI GCGC 2 cut(s) 1129, 1149
AspS9I GGNCC 3 cut(s) 355, 746, 998
AvaII GGWCC 2 cut(s) 746, 998
BaeGI GKGCMC 1 cut(s) 456
BaeI ACNNNNGTAYC 2 cut(s) 794, 827
BamHI GGATCC 2 cut(s) 625, 937
BanII GRGCYC 1 cut(s) 23
BbsI GAAGAC 1 cut(s) 215
Bbv12I GWGCWC 2 cut(s) 23, 76
BbvI GCAGC 2 cut(s) 257, 438
BccI CCATC 3 cut(s) 349, 521, 883
BcgI CGANNNNNNTGC 2 cut(s) 946, 980
BciT130I CCWGG 2 cut(s) 441, 750
BciVI GTATCC 1 cut(s) 248
BcoDI GTCTC 3 cut(s) 294, 669, 1110
BfaI CTAG 3 cut(s) 392, 471, 983
BfuAI ACCTGC 1 cut(s) 734
BfuI GTATCC 1 cut(s) 248
BisI GCNGC 2 cut(s) 246, 427
BlsI GCNGC 2 cut(s) 247, 428
Bme1390I CCNGG 2 cut(s) 441, 750
Bme18I GGWCC 2 cut(s) 746, 998
BmgBI CACGTC 1 cut(s) 705
BmgT120I GGNCC 3 cut(s) 355, 746, 998
BmiI GGNNCC 2 cut(s) 627, 939
BmrFI CCNGG 2 cut(s) 441, 750
BmrI ACTGGG 1 cut(s) 1167
BmsI GCATC 2 cut(s) 211, 397
BmuI ACTGGG 1 cut(s) 1167
BpiI GAAGAC 1 cut(s) 215
BsaBI GATNNNNATC 1 cut(s) 930
BsaJI CCNNGG 1 cut(s) 669
Bsc4I CCNNNNNNNGG 1 cut(s) 155
Bse118I RCCGGY 1 cut(s) 1044
Bse1I ACTGG 2 cut(s) 241, 1162
Bse3DI GCAATG 2 cut(s) 569, 661
Bse8I GATNNNNATC 1 cut(s) 930
BseBI CCWGG 2 cut(s) 441, 750
BseDI CCNNGG 1 cut(s) 669
BseGI GGATG 4 cut(s) 226, 801, 869, 870
BseJI GATNNNNATC 1 cut(s) 930
BseLI CCNNNNNNNGG 1 cut(s) 155
BseMI GCAATG 2 cut(s) 569, 661
BseNI ACTGG 2 cut(s) 241, 1162
BseRI GAGGAG 2 cut(s) 40, 1170
BseSI GKGCMC 1 cut(s) 456
BseXI GCAGC 2 cut(s) 257, 438
BshFI GGCC 3 cut(s) 356, 589, 1044
BsiHKAI GWGCWC 2 cut(s) 23, 76
BsiSI CCGG 1 cut(s) 1045
BslFI GGGAC 1 cut(s) 630
BslI CCNNNNNNNGG 1 cut(s) 155
BsmAI GTCTC 3 cut(s) 294, 669, 1110
BsmFI GGGAC 1 cut(s) 630
BsnI GGCC 3 cut(s) 356, 589, 1044
Bsp1286I GDGCHC 3 cut(s) 23, 76, 456
Bsp1407I TGTACA 1 cut(s) 802
Bsp143I GATC 6 cut(s) 66, 625, 715, 763, 937, 968
BspACI CCGC 1 cut(s) 218
BspANI GGCC 3 cut(s) 356, 589, 1044
BspHI TCATGA 1 cut(s) 840
BspLI GGNNCC 2 cut(s) 627, 939
BspMI ACCTGC 1 cut(s) 734
BspPI GGATC 6 cut(s) 620, 633, 758, 932, 945, 963
BspQI GCTCTTC 1 cut(s) 453
BsrDI GCAATG 2 cut(s) 569, 661
BsrFI RCCGGY 1 cut(s) 1044
BsrGI TGTACA 1 cut(s) 802
BsrI ACTGG 2 cut(s) 241, 1162
BssAI RCCGGY 1 cut(s) 1044
BssECI CCNNGG 1 cut(s) 669
BssMI GATC 6 cut(s) 66, 625, 715, 763, 937, 968
Bst2UI CCWGG 2 cut(s) 441, 750
Bst4CI ACNGT 6 cut(s) 113, 167, 205, 305, 1066, 1111
Bst6I CTCTTC 2 cut(s) 453, 900
BstAPI GCANNNNNTGC 1 cut(s) 1175
BstAUI TGTACA 1 cut(s) 802
BstC8I GCNNGC 2 cut(s) 883, 1151
BstDSI CCRYGG 1 cut(s) 669
BstEII GGTNACC 1 cut(s) 311
BstF5I GGATG 4 cut(s) 226, 801, 869, 870
BstHHI GCGC 2 cut(s) 1129, 1149
BstKTI GATC 6 cut(s) 69, 628, 718, 766, 940, 971
BstMAI GTCTC 3 cut(s) 294, 669, 1110
BstMBI GATC 6 cut(s) 66, 625, 715, 763, 937, 968
BstMWI GCNNNNNNNGC 5 cut(s) 460, 638, 740, 1159, 1175
BstNI CCWGG 2 cut(s) 441, 750
BstNSI RCATGY 2 cut(s) 775, 1153
BstPI GGTNACC 1 cut(s) 311
BstSCI CCNGG 2 cut(s) 439, 748
BstSLI GKGCMC 1 cut(s) 456
BstV1I GCAGC 2 cut(s) 257, 438
BstV2I GAAGAC 1 cut(s) 215
BstX2I RGATCY 2 cut(s) 625, 937
BstXI CCANNNNNNTGG 1 cut(s) 159
BstYI RGATCY 2 cut(s) 625, 937
BsuI GTATCC 1 cut(s) 248
BsuRI GGCC 3 cut(s) 356, 589, 1044
BtgI CCRYGG 1 cut(s) 669
BtrI CACGTC 1 cut(s) 705
BtsCI GGATG 4 cut(s) 226, 801, 869, 870
BtsI GCAGTG 1 cut(s) 213
BtsIMutI CAGTG 3 cut(s) 210, 213, 1155
BveI ACCTGC 1 cut(s) 734
Cac8I GCNNGC 2 cut(s) 883, 1151
CaiI CAGNNNCTG 1 cut(s) 749
CciI TCATGA 1 cut(s) 840
CfoI GCGC 2 cut(s) 1129, 1149
Cfr10I RCCGGY 1 cut(s) 1044
Cfr13I GGNCC 3 cut(s) 355, 746, 998
CseI GACGC 1 cut(s) 476
Csp6I GTAC 5 cut(s) 144, 508, 598, 803, 811
CviAII CATG 5 cut(s) 772, 841, 1071, 1150, 1184
CviQI GTAC 5 cut(s) 144, 508, 598, 803, 811
DpnI GATC 6 cut(s) 68, 627, 717, 765, 939, 970
DpnII GATC 6 cut(s) 66, 625, 715, 763, 937, 968
Eam1104I CTCTTC 2 cut(s) 453, 900
EarI CTCTTC 2 cut(s) 453, 900
Ecl136II GAGCTC 1 cut(s) 21
Eco24I GRGCYC 1 cut(s) 23
Eco32I GATATC 1 cut(s) 551
Eco47I GGWCC 2 cut(s) 746, 998
Eco53kI GAGCTC 1 cut(s) 21
Eco57I CTGAAG 1 cut(s) 881
Eco91I GGTNACC 1 cut(s) 311
EcoICRI GAGCTC 1 cut(s) 21
EcoO109I RGGNCCY 2 cut(s) 355, 746
EcoO65I GGTNACC 1 cut(s) 311
EcoRII CCWGG 2 cut(s) 439, 748
EcoRV GATATC 1 cut(s) 551
EcoT38I GRGCYC 1 cut(s) 23
FaeI CATG 5 cut(s) 775, 844, 1074, 1153, 1187
FaqI GGGAC 1 cut(s) 630
FatI CATG 5 cut(s) 771, 840, 1070, 1149, 1183
FauNDI CATATG 2 cut(s) 605, 739
Fnu4HI GCNGC 2 cut(s) 246, 427
FokI GGATG 4 cut(s) 233, 808, 857, 876
FriOI GRGCYC 1 cut(s) 23
Fsp4HI GCNGC 2 cut(s) 246, 427
FspBI CTAG 3 cut(s) 392, 471, 983
GlaI GCGC 2 cut(s) 1128, 1148
GluI GCNGC 2 cut(s) 246, 427
HaeIII GGCC 3 cut(s) 356, 589, 1044
HapII CCGG 1 cut(s) 1045
HgaI GACGC 1 cut(s) 476
HhaI GCGC 2 cut(s) 1129, 1149
Hin1II CATG 5 cut(s) 775, 844, 1074, 1153, 1187
Hin6I GCGC 2 cut(s) 1127, 1147
HinP1I GCGC 2 cut(s) 1127, 1147
HincII GTYRAC 1 cut(s) 333
HindII GTYRAC 1 cut(s) 333
HinfI GANTC 5 cut(s) 172, 348, 901, 1086, 1207
HpaII CCGG 1 cut(s) 1045
Hpy166II GTNNAC 5 cut(s) 146, 333, 373, 386, 803
Hpy188I TCNGA 8 cut(s) 78, 406, 548, 863, 900, 908, 947, 1115
Hpy188III TCNNGA 5 cut(s) 345, 767, 841, 935, 1204
Hpy8I GTNNAC 5 cut(s) 146, 333, 373, 386, 803
Hpy99I CGWCG 1 cut(s) 709
HpyAV CCTTC 2 cut(s) 579, 1069
HpyCH4III ACNGT 6 cut(s) 113, 167, 205, 305, 1066, 1111
HpyCH4IV ACGT 1 cut(s) 704
HpyCH4V TGCA 6 cut(s) 186, 426, 569, 713, 743, 1169
HpyF10VI GCNNNNNNNGC 5 cut(s) 460, 638, 740, 1159, 1175
HpySE526I ACGT 1 cut(s) 704
Hsp92II CATG 5 cut(s) 775, 844, 1074, 1153, 1187
HspAI GCGC 2 cut(s) 1127, 1147
Kzo9I GATC 6 cut(s) 66, 625, 715, 763, 937, 968
LguI GCTCTTC 1 cut(s) 453
LmnI GCTCC 4 cut(s) 18, 853, 1060, 1183
Lsp1109I GCAGC 2 cut(s) 257, 438
LweI GCATC 2 cut(s) 211, 397
MaeI CTAG 3 cut(s) 392, 471, 983
MaeII ACGT 1 cut(s) 704
MaeIII GTNAC 4 cut(s) 311, 866, 1066, 1105
MalI GATC 6 cut(s) 68, 627, 717, 765, 939, 970
MboI GATC 6 cut(s) 66, 625, 715, 763, 937, 968
MboII GAAGA 4 cut(s) 97, 220, 470, 887
MflI RGATCY 2 cut(s) 625, 937
MhlI GDGCHC 3 cut(s) 23, 76, 456
MlyI GAGTC 2 cut(s) 1080, 1201
MmeI TCCRAC 4 cut(s) 140, 149, 756, 1063
MroXI GAANNNNTTC 2 cut(s) 176, 894
MseI TTAA 3 cut(s) 681, 930, 1213
MspI CCGG 1 cut(s) 1045
MspR9I CCNGG 2 cut(s) 441, 750
MvaI CCWGG 2 cut(s) 441, 750
MwoI GCNNNNNNNGC 5 cut(s) 460, 638, 740, 1159, 1175
NdeI CATATG 2 cut(s) 605, 739
NdeII GATC 6 cut(s) 66, 625, 715, 763, 937, 968
NlaIII CATG 5 cut(s) 775, 844, 1074, 1153, 1187
NlaIV GGNNCC 2 cut(s) 627, 939
NmuCI GTSAC 2 cut(s) 866, 1066
NspI RCATGY 2 cut(s) 775, 1153
PaeI GCATGC 1 cut(s) 1153
PagI TCATGA 1 cut(s) 840
PciI ACATGT 1 cut(s) 771
PciSI GCTCTTC 1 cut(s) 453
PdmI GAANNNNTTC 2 cut(s) 176, 894
PfeI GAWTC 3 cut(s) 172, 348, 901
PkrI GCNGC 2 cut(s) 247, 428
PleI GAGTC 2 cut(s) 1080, 1201
PpsI GAGTC 2 cut(s) 1080, 1201
PpuMI RGGWCCY 1 cut(s) 746
PscI ACATGT 1 cut(s) 771
Psp124BI GAGCTC 1 cut(s) 23
Psp5II RGGWCCY 1 cut(s) 746
Psp6I CCWGG 2 cut(s) 439, 748
PspEI GGTNACC 1 cut(s) 311
PspGI CCWGG 2 cut(s) 439, 748
PspN4I GGNNCC 2 cut(s) 627, 939
PspPI GGNCC 3 cut(s) 355, 746, 998
PspPPI RGGWCCY 1 cut(s) 746
PstNI CAGNNNCTG 1 cut(s) 749
PsuI RGATCY 2 cut(s) 625, 937
RsaI GTAC 5 cut(s) 145, 509, 599, 804, 812
RsaNI GTAC 5 cut(s) 144, 508, 598, 803, 811
SacI GAGCTC 1 cut(s) 23
SapI GCTCTTC 1 cut(s) 453
SaqAI TTAA 3 cut(s) 681, 930, 1213
SatI GCNGC 2 cut(s) 246, 427
Sau3AI GATC 6 cut(s) 66, 625, 715, 763, 937, 968
Sau96I GGNCC 3 cut(s) 355, 746, 998
SchI GAGTC 2 cut(s) 1080, 1201
ScrFI CCNGG 2 cut(s) 441, 750
SduI GDGCHC 3 cut(s) 23, 76, 456
SfaNI GCATC 2 cut(s) 211, 397
SinI GGWCC 2 cut(s) 746, 998
SphI GCATGC 1 cut(s) 1153
SsiI CCGC 1 cut(s) 218
SspMI CTAG 3 cut(s) 392, 471, 983
SstI GAGCTC 1 cut(s) 23
StyD4I CCNGG 2 cut(s) 439, 748
TaaI ACNGT 6 cut(s) 113, 167, 205, 305, 1066, 1111
TaiI ACGT 1 cut(s) 707
TaqI TCGA 3 cut(s) 233, 495, 707
TatI WGTACW 2 cut(s) 597, 802
TfiI GAWTC 3 cut(s) 172, 348, 901
Tru1I TTAA 3 cut(s) 681, 930, 1213
Tru9I TTAA 3 cut(s) 681, 930, 1213
TscAI CASTG 3 cut(s) 210, 220, 1162
TseFI GTSAC 2 cut(s) 866, 1066
TseI GCWGC 2 cut(s) 245, 426
Tsp45I GTSAC 2 cut(s) 866, 1066
TspDTI ATGAA 2 cut(s) 210, 857
TspRI CASTG 3 cut(s) 210, 220, 1162
VpaK11BI GGWCC 2 cut(s) 746, 998
XceI RCATGY 2 cut(s) 775, 1153
XmnI GAANNNNTTC 2 cut(s) 176, 894
XspI CTAG 3 cut(s) 392, 471, 983
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.