pycom05g29610

Alpha galactosidase A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0009821
Physical Location & Seq
Forward (+)
30015032 .. 30017677
2646 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g29610.7

Sequence Viewer

Length: 1215 bp
ATGGATTGGAAACGCAGGAGCTCACTCTTTGTTCTGCTCTTTGTTATCTCGTCTATTTTCAATGTGATTGATGCTCTGAACTTGATGAATAATACTCACCAAGCAGAATACGGTCAGTTCCTCCTTGCCAATGGCGTTGCTACTACACCTCCAATGGGTTGGAACAGTTGGAATCACTTCCAATGCAATATAAGTGAAGCCACAGTGAAGACCACTGCGGATGCTCTTGTTTCAACTGGCTTGGCAGCACTCGGATACAAATATGTCAATATAGATGATTGTTGGGCTGAAAGAAACAGAGACAGTAGTGGTAACCTAATAGCAAAATCGTCAACTTTTCCATCTGGAATCAAGGCCCTTGCTGATTATGTTCACGCAAGGGGTTTACAACTCGGCATATACTCTGATGCTGGTTATTATACTTGCAGCAACACAATGCCAGGCTCACTTGGGCACGAAGAGCAAGACGCAAGAACCTTTGCTGAATGGGGTGTCGATTATTTGAAGTACGATAACTGCAACCATGATGGTTCTAAACCTCAAATCAGATATCCTATAATGAGTTCTGCATTGCGAAAGGCTGGAAGGCCAATCCTGTACTCCATATGCGAATGGGGACAAGAGGATCCTGCGAAATGGGCTGGTCAGTATGGAAATGTGTGGAGAACTACGGGAGACATTCAGGACACTTGGGAAAGCATCACGTCGATTGCAGATCAAAACAACATTTGGAAGACATATGCGGGCCCTGGCAAGTGGAACGATCCTGACATGTTGGAAGTGGGAAATGGAGGGATGAGTGTAGAGGAGTATCGATCTCATTTTAGCATTTGGGCAGTCATGAAAGCTCCTTTACTTATCGGATGTGACATCCCATCTGCGAGCAAGGAAACTCTTCAGATTCTTGGAAATAAAGAGGTTATTGATGTTAATCAGGATCCACTCGGAGTTCAAGCGGGGAAACTGCGATCCAAAGATAACCTAGAAGTGTGGGCAGGACCATTATCAAATAAAAGAGTGGTGGTAGTGTTGTGGAATAGAGGCGGGTCATTAGCTCCTATAACTGTGACATGGAAGGAAGTTGGACTCTCGCCAAATACACCTATTACAGTCAGAGACTTATGGGCGCACTCATTTGTTTCAAGGAGCATGCTTACTGGGCTGACTGCAAATGTTGCTCCTCATGGTTGTAAGATGTATGTCCTGACTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 ko00561 ko00600 ko00603 map00052 map00561 map00600 map00603
Pfam Domains
Protein Families
Melibiase_2 Melibiase_2_C

Protein Analysis

405

Amino Acids

44.67

Weight (kDa)

6.08

Isoelectric Point (pI)

36.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 218, 743, 956, 1044
AclWI GGATC 6 cut(s) 620, 633, 758, 932, 945, 963
AcuI CTGAAG 1 cut(s) 881
AfaI GTAC 2 cut(s) 509, 599
AfiI CCNNNNNNNGG 1 cut(s) 155
AflIII ACRYGT 1 cut(s) 771
AgsI TTSAA 5 cut(s) 61, 234, 505, 953, 1143
AjiI CACGTC 1 cut(s) 705
AjnI CCWGG 2 cut(s) 439, 748
AluBI AGCT 3 cut(s) 21, 848, 1055
AluI AGCT 3 cut(s) 21, 848, 1055
Alw21I GWGCWC 1 cut(s) 23
Alw26I GTCTC 3 cut(s) 294, 669, 1110
AlwI GGATC 6 cut(s) 620, 633, 758, 932, 945, 963
AoxI GGCC 3 cut(s) 354, 587, 745
ApaI GGGCCC 1 cut(s) 749
ApeKI GCWGC 2 cut(s) 245, 426
Asp700I GAANNNNTTC 2 cut(s) 176, 894
AspLEI GCGC 1 cut(s) 1129
AspS9I GGNCC 4 cut(s) 355, 745, 746, 998
AsuHPI GGTGA 1 cut(s) 89
AvaII GGWCC 1 cut(s) 998
BaeGI GKGCMC 2 cut(s) 456, 749
BamHI GGATCC 2 cut(s) 625, 937
BanII GRGCYC 2 cut(s) 23, 749
BbsI GAAGAC 2 cut(s) 215, 740
Bbv12I GWGCWC 1 cut(s) 23
BbvI GCAGC 2 cut(s) 257, 438
BccI CCATC 3 cut(s) 349, 521, 883
BciT130I CCWGG 2 cut(s) 441, 750
BciVI GTATCC 1 cut(s) 248
BcoDI GTCTC 3 cut(s) 294, 669, 1110
BfaI CTAG 1 cut(s) 983
BfuI GTATCC 1 cut(s) 248
BisI GCNGC 2 cut(s) 246, 427
BlsI GCNGC 2 cut(s) 247, 428
Bme1390I CCNGG 2 cut(s) 441, 750
Bme18I GGWCC 1 cut(s) 998
BmgBI CACGTC 1 cut(s) 705
BmgT120I GGNCC 4 cut(s) 355, 745, 746, 998
BmiI GGNNCC 3 cut(s) 627, 747, 939
BmrFI CCNGG 2 cut(s) 441, 750
BmrI ACTGGG 1 cut(s) 1167
BmsI GCATC 4 cut(s) 61, 211, 397, 708
BmuI ACTGGG 1 cut(s) 1167
BpiI GAAGAC 2 cut(s) 215, 740
Bsa29I ATCGAT 1 cut(s) 814
BsaBI GATNNNNATC 1 cut(s) 930
BsaJI CCNNGG 1 cut(s) 748
BsaXI ACNNNNNCTCC 2 cut(s) 133, 163
Bsc4I CCNNNNNNNGG 1 cut(s) 155
Bse1I ACTGG 2 cut(s) 241, 1162
Bse3DI GCAATG 1 cut(s) 569
Bse8I GATNNNNATC 1 cut(s) 930
BseBI CCWGG 2 cut(s) 441, 750
BseCI ATCGAT 1 cut(s) 814
BseDI CCNNGG 1 cut(s) 748
BseGI GGATG 4 cut(s) 226, 801, 869, 870
BseJI GATNNNNATC 1 cut(s) 930
BseLI CCNNNNNNNGG 1 cut(s) 155
BseMI GCAATG 1 cut(s) 569
BseNI ACTGG 2 cut(s) 241, 1162
BseRI GAGGAG 2 cut(s) 821, 1170
BseSI GKGCMC 2 cut(s) 456, 749
BseXI GCAGC 2 cut(s) 257, 438
BshFI GGCC 3 cut(s) 356, 589, 747
BshVI ATCGAT 1 cut(s) 814
BsiHKAI GWGCWC 1 cut(s) 23
BslFI GGGAC 1 cut(s) 630
BslI CCNNNNNNNGG 1 cut(s) 155
BsmAI GTCTC 3 cut(s) 294, 669, 1110
BsmFI GGGAC 1 cut(s) 630
BsnI GGCC 3 cut(s) 356, 589, 747
Bsp120I GGGCCC 1 cut(s) 745
Bsp1286I GDGCHC 3 cut(s) 23, 456, 749
Bsp143I GATC 6 cut(s) 625, 715, 763, 815, 937, 968
BspACI CCGC 4 cut(s) 218, 743, 956, 1044
BspANI GGCC 3 cut(s) 356, 589, 747
BspDI ATCGAT 1 cut(s) 814
BspHI TCATGA 1 cut(s) 840
BspLI GGNNCC 3 cut(s) 627, 747, 939
BspPI GGATC 6 cut(s) 620, 633, 758, 932, 945, 963
BspQI GCTCTTC 1 cut(s) 453
BsrDI GCAATG 1 cut(s) 569
BsrI ACTGG 2 cut(s) 241, 1162
BssECI CCNNGG 1 cut(s) 748
BssMI GATC 6 cut(s) 625, 715, 763, 815, 937, 968
Bst2UI CCWGG 2 cut(s) 441, 750
Bst4CI ACNGT 6 cut(s) 113, 167, 205, 305, 1066, 1111
Bst6I CTCTTC 2 cut(s) 453, 900
BstAPI GCANNNNNTGC 1 cut(s) 1175
BstC8I GCNNGC 3 cut(s) 745, 883, 1151
BstEII GGTNACC 1 cut(s) 311
BstF5I GGATG 4 cut(s) 226, 801, 869, 870
BstHHI GCGC 1 cut(s) 1129
BstKTI GATC 6 cut(s) 628, 718, 766, 818, 940, 971
BstMAI GTCTC 3 cut(s) 294, 669, 1110
BstMBI GATC 6 cut(s) 625, 715, 763, 815, 937, 968
BstMWI GCNNNNNNNGC 4 cut(s) 460, 638, 1159, 1175
BstNI CCWGG 2 cut(s) 441, 750
BstNSI RCATGY 2 cut(s) 775, 1153
BstPI GGTNACC 1 cut(s) 311
BstSCI CCNGG 2 cut(s) 439, 748
BstSLI GKGCMC 2 cut(s) 456, 749
BstV1I GCAGC 2 cut(s) 257, 438
BstV2I GAAGAC 2 cut(s) 215, 740
BstX2I RGATCY 2 cut(s) 625, 937
BstXI CCANNNNNNTGG 1 cut(s) 159
BstYI RGATCY 2 cut(s) 625, 937
Bsu15I ATCGAT 1 cut(s) 814
BsuI GTATCC 1 cut(s) 248
BsuRI GGCC 3 cut(s) 356, 589, 747
BsuTUI ATCGAT 1 cut(s) 814
BtrI CACGTC 1 cut(s) 705
BtsCI GGATG 4 cut(s) 226, 801, 869, 870
BtsI GCAGTG 1 cut(s) 213
BtsIMutI CAGTG 2 cut(s) 210, 213
Cac8I GCNNGC 3 cut(s) 745, 883, 1151
CciI TCATGA 1 cut(s) 840
CfoI GCGC 1 cut(s) 1129
Cfr13I GGNCC 4 cut(s) 355, 745, 746, 998
ClaI ATCGAT 1 cut(s) 814
CseI GACGC 1 cut(s) 476
Csp6I GTAC 2 cut(s) 508, 598
CviAII CATG 6 cut(s) 524, 772, 841, 1071, 1150, 1184
CviQI GTAC 2 cut(s) 508, 598
DpnI GATC 6 cut(s) 627, 717, 765, 817, 939, 970
DpnII GATC 6 cut(s) 625, 715, 763, 815, 937, 968
Eam1104I CTCTTC 2 cut(s) 453, 900
EarI CTCTTC 2 cut(s) 453, 900
Ecl136II GAGCTC 1 cut(s) 21
Eco24I GRGCYC 2 cut(s) 23, 749
Eco32I GATATC 1 cut(s) 551
Eco47I GGWCC 1 cut(s) 998
Eco53kI GAGCTC 1 cut(s) 21
Eco57I CTGAAG 1 cut(s) 881
Eco91I GGTNACC 1 cut(s) 311
EcoICRI GAGCTC 1 cut(s) 21
EcoO109I RGGNCCY 2 cut(s) 355, 746
EcoO65I GGTNACC 1 cut(s) 311
EcoRII CCWGG 2 cut(s) 439, 748
EcoRV GATATC 1 cut(s) 551
EcoT38I GRGCYC 2 cut(s) 23, 749
FaeI CATG 6 cut(s) 527, 775, 844, 1074, 1153, 1187
FaqI GGGAC 1 cut(s) 630
FatI CATG 6 cut(s) 523, 771, 840, 1070, 1149, 1183
FauI CCCGC 3 cut(s) 736, 949, 1037
FauNDI CATATG 2 cut(s) 605, 739
Fnu4HI GCNGC 2 cut(s) 246, 427
FokI GGATG 4 cut(s) 233, 808, 857, 876
FriOI GRGCYC 2 cut(s) 23, 749
Fsp4HI GCNGC 2 cut(s) 246, 427
FspBI CTAG 1 cut(s) 983
GlaI GCGC 1 cut(s) 1128
GluI GCNGC 2 cut(s) 246, 427
HaeIII GGCC 3 cut(s) 356, 589, 747
HgaI GACGC 1 cut(s) 476
HhaI GCGC 1 cut(s) 1129
Hin1II CATG 6 cut(s) 527, 775, 844, 1074, 1153, 1187
Hin6I GCGC 1 cut(s) 1127
HinP1I GCGC 1 cut(s) 1127
HincII GTYRAC 1 cut(s) 333
HindII GTYRAC 1 cut(s) 333
HinfI GANTC 5 cut(s) 172, 348, 901, 1086, 1207
HphI GGTGA 1 cut(s) 89
Hpy166II GTNNAC 3 cut(s) 333, 373, 386
Hpy188I TCNGA 8 cut(s) 78, 254, 406, 548, 863, 900, 947, 1115
Hpy188III TCNNGA 6 cut(s) 345, 683, 767, 841, 935, 1204
Hpy8I GTNNAC 3 cut(s) 333, 373, 386
Hpy99I CGWCG 1 cut(s) 709
HpyAV CCTTC 2 cut(s) 579, 1069
HpyCH4III ACNGT 6 cut(s) 113, 167, 205, 305, 1066, 1111
HpyCH4IV ACGT 1 cut(s) 704
HpyCH4V TGCA 6 cut(s) 186, 426, 519, 569, 713, 1169
HpyF10VI GCNNNNNNNGC 4 cut(s) 460, 638, 1159, 1175
HpySE526I ACGT 1 cut(s) 704
Hsp92II CATG 6 cut(s) 527, 775, 844, 1074, 1153, 1187
HspAI GCGC 1 cut(s) 1127
Kzo9I GATC 6 cut(s) 625, 715, 763, 815, 937, 968
LguI GCTCTTC 1 cut(s) 453
LmnI GCTCC 5 cut(s) 18, 853, 1060, 1146, 1183
Lsp1109I GCAGC 2 cut(s) 257, 438
LweI GCATC 4 cut(s) 61, 211, 397, 708
MaeI CTAG 1 cut(s) 983
MaeII ACGT 1 cut(s) 704
MaeIII GTNAC 3 cut(s) 311, 866, 1066
MalI GATC 6 cut(s) 627, 717, 765, 817, 939, 970
MboI GATC 6 cut(s) 625, 715, 763, 815, 937, 968
MboII GAAGA 4 cut(s) 220, 470, 745, 887
MflI RGATCY 2 cut(s) 625, 937
MhlI GDGCHC 3 cut(s) 23, 456, 749
MlyI GAGTC 2 cut(s) 1080, 1201
MmeI TCCRAC 4 cut(s) 140, 149, 756, 1063
MnlI CCTC 9 cut(s) 131, 159, 549, 616, 785, 799, 910, 1034, 1191
MroXI GAANNNNTTC 2 cut(s) 176, 894
MseI TTAA 2 cut(s) 930, 1213
MspR9I CCNGG 2 cut(s) 441, 750
MvaI CCWGG 2 cut(s) 441, 750
MwoI GCNNNNNNNGC 4 cut(s) 460, 638, 1159, 1175
NdeI CATATG 2 cut(s) 605, 739
NdeII GATC 6 cut(s) 625, 715, 763, 815, 937, 968
NlaIII CATG 6 cut(s) 527, 775, 844, 1074, 1153, 1187
NlaIV GGNNCC 3 cut(s) 627, 747, 939
NmeAIII GCCGAG 1 cut(s) 372
NmuCI GTSAC 2 cut(s) 866, 1066
NspI RCATGY 2 cut(s) 775, 1153
PaeI GCATGC 1 cut(s) 1153
PagI TCATGA 1 cut(s) 840
PciI ACATGT 1 cut(s) 771
PciSI GCTCTTC 1 cut(s) 453
PdmI GAANNNNTTC 2 cut(s) 176, 894
PfeI GAWTC 3 cut(s) 172, 348, 901
PkrI GCNGC 2 cut(s) 247, 428
PleI GAGTC 2 cut(s) 1080, 1201
PpsI GAGTC 2 cut(s) 1080, 1201
PscI ACATGT 1 cut(s) 771
Psp124BI GAGCTC 1 cut(s) 23
Psp6I CCWGG 2 cut(s) 439, 748
PspEI GGTNACC 1 cut(s) 311
PspGI CCWGG 2 cut(s) 439, 748
PspN4I GGNNCC 3 cut(s) 627, 747, 939
PspOMI GGGCCC 1 cut(s) 745
PspPI GGNCC 4 cut(s) 355, 745, 746, 998
PsuI RGATCY 2 cut(s) 625, 937
RsaI GTAC 2 cut(s) 509, 599
RsaNI GTAC 2 cut(s) 508, 598
SacI GAGCTC 1 cut(s) 23
SapI GCTCTTC 1 cut(s) 453
SaqAI TTAA 2 cut(s) 930, 1213
SatI GCNGC 2 cut(s) 246, 427
Sau3AI GATC 6 cut(s) 625, 715, 763, 815, 937, 968
Sau96I GGNCC 4 cut(s) 355, 745, 746, 998
SchI GAGTC 2 cut(s) 1080, 1201
ScrFI CCNGG 2 cut(s) 441, 750
SduI GDGCHC 3 cut(s) 23, 456, 749
SfaNI GCATC 4 cut(s) 61, 211, 397, 708
SinI GGWCC 1 cut(s) 998
SphI GCATGC 1 cut(s) 1153
SsiI CCGC 4 cut(s) 218, 743, 956, 1044
SspMI CTAG 1 cut(s) 983
SstI GAGCTC 1 cut(s) 23
StyD4I CCNGG 2 cut(s) 439, 748
TaaI ACNGT 6 cut(s) 113, 167, 205, 305, 1066, 1111
TaiI ACGT 1 cut(s) 707
TaqI TCGA 3 cut(s) 495, 707, 814
TatI WGTACW 1 cut(s) 597
TfiI GAWTC 3 cut(s) 172, 348, 901
Tru1I TTAA 2 cut(s) 930, 1213
Tru9I TTAA 2 cut(s) 930, 1213
TscAI CASTG 2 cut(s) 210, 220
TseFI GTSAC 2 cut(s) 866, 1066
TseI GCWGC 2 cut(s) 245, 426
Tsp45I GTSAC 2 cut(s) 866, 1066
TspDTI ATGAA 2 cut(s) 101, 857
TspRI CASTG 2 cut(s) 210, 220
VpaK11BI GGWCC 1 cut(s) 998
XceI RCATGY 2 cut(s) 775, 1153
XmnI GAANNNNTTC 2 cut(s) 176, 894
XspI CTAG 1 cut(s) 983
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.