MD05G1321600.v1.1

Alpha galactosidase A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0009821
Physical Location & Seq
Forward (+)
44914120 .. 44917254
3135 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1321600.v1.1.491

Sequence Viewer

Length: 960 bp
ATGAGAACTAAAAAAGCAGATGATTGTTGGGCTGAAAGAAACAGAGACAGTAGTGGTAACCTAATAGCAAAATCGTCAACTTTTCCATCTGGAATCAAGGCCCTTGCTGATTATGTTCACGCAAGGGGTTTACAGCTAGGCATATACTCTGATGCTGGTCATTATACTTGCAGCAACACAATGCCAGGCTCACTTGGGCACGAAGAGCAAGACGCTAGAACCTTTGCTGAATGGGGTGTCGATTATTTGAAGTACGATAACTGCAACCATGATGGTTCTAAACCTCAAATCAGATATCCTGTAATGAGTTCTGCATTGCTGAAGACTGGAAGGCCAATCCTGTACTCCATATGCGAATGGGGACAAGAGGATCCTGCGACATGGGCTGGTCAGTATGGCAATGCTTGGAGAACTACGGGAGACATTGAGGACACTTGGGAAAGTATCACGTCGATTGCAGACCAAAACAACATTTGGGCTACTTATGCAGGCCCTGGCAAGTGGAATGATCCTGACATGTTGGAAGTGGGAAACGGAGGGATGAGTGTAGAGGAGTACCAATCTCATTTTAGCATTTGGGCAGTCATGAAAGCTCCTTTACTTATCGGATGTGACATCCCATCTGCGAGCAAGGAAGCTCTTCAGATTCTCGGAAACAAAGAGGTTATTGATGTTAATCAAGATTCACTTGGAGTTCAAGCGAGGAAACTGCGATCCAGAGATGACCTAGAAGTGTGGGCAGGACCGTTATCAAGTAACAGAGTGGTGGTAGTGTTGTGGAATAGAGGCGGGTCATTAGCTCCTATAACTGTGACATGGAGGGAAATTGGACTCTCGCCAAATACGTCTGTCATTGTCAGAGACTTATGGACGCACTCATTTGTTTCGAAGAACATGCATACTAGTCTGACTGCAAATGTTGCTCCTCATGGTTGTAATATGTATGTCCTGACTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 ko00561 ko00600 ko00603 map00052 map00561 map00600 map00603
Pfam Domains
Protein Families
Melibiase_2 Melibiase_2_C

Protein Analysis

320

Amino Acids

35.24

Weight (kDa)

5.49

Isoelectric Point (pI)

37.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Melibiase_2 PF16499 6 - 228 6.2e-70 Alpha galactosidase A
Melibiase_2_C PF17450 231 - 311 6.9e-06 Alpha galactosidase A C-terminal beta sandwich domain
Melibiase_C PF17801 241 - 317 8.5e-20 Alpha galactosidase C-terminal beta sandwich domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 789
AclWI GGATC 4 cut(s) 365, 378, 503, 708
AcuI CTGAAG 2 cut(s) 341, 626
AfaI GTAC 3 cut(s) 254, 344, 557
AflIII ACRYGT 1 cut(s) 516
AgsI TTSAA 2 cut(s) 250, 698
AhlI ACTAGT 1 cut(s) 902
AjiI CACGTC 1 cut(s) 450
AjnI CCWGG 2 cut(s) 184, 493
AluBI AGCT 4 cut(s) 136, 593, 638, 800
AluI AGCT 4 cut(s) 136, 593, 638, 800
Alw26I GTCTC 3 cut(s) 39, 414, 855
AlwI GGATC 4 cut(s) 365, 378, 503, 708
AlwNI CAGNNNCTG 1 cut(s) 494
AoxI GGCC 3 cut(s) 99, 332, 490
ApeKI GCWGC 1 cut(s) 171
Asp700I GAANNNNTTC 1 cut(s) 639
AspS9I GGNCC 3 cut(s) 100, 491, 743
AsuII TTCGAA 1 cut(s) 887
AvaII GGWCC 1 cut(s) 743
BaeGI GKGCMC 1 cut(s) 201
BamHI GGATCC 1 cut(s) 370
BbsI GAAGAC 1 cut(s) 329
BbvI GCAGC 1 cut(s) 183
BccI CCATC 3 cut(s) 94, 266, 628
BcgI CGANNNNNNTGC 4 cut(s) 691, 725, 877, 911
BciT130I CCWGG 2 cut(s) 186, 495
BcoDI GTCTC 3 cut(s) 39, 414, 855
BcuI ACTAGT 1 cut(s) 902
BfaI CTAG 4 cut(s) 137, 216, 728, 903
BisI GCNGC 1 cut(s) 172
BlsI GCNGC 1 cut(s) 173
Bme1390I CCNGG 2 cut(s) 186, 495
Bme18I GGWCC 1 cut(s) 743
BmgBI CACGTC 1 cut(s) 450
BmgT120I GGNCC 3 cut(s) 100, 491, 743
BmiI GGNNCC 1 cut(s) 372
BmrFI CCNGG 2 cut(s) 186, 495
BmsI GCATC 1 cut(s) 142
BpiI GAAGAC 1 cut(s) 329
Bpu14I TTCGAA 1 cut(s) 887
BsaBI GATNNNNATC 1 cut(s) 675
BsaJI CCNNGG 1 cut(s) 493
Bse1I ACTGG 1 cut(s) 331
Bse3DI GCAATG 2 cut(s) 314, 406
Bse8I GATNNNNATC 1 cut(s) 675
BseBI CCWGG 2 cut(s) 186, 495
BseDI CCNNGG 1 cut(s) 493
BseGI GGATG 3 cut(s) 546, 614, 615
BseJI GATNNNNATC 1 cut(s) 675
BseMI GCAATG 2 cut(s) 314, 406
BseNI ACTGG 1 cut(s) 331
BseRI GAGGAG 2 cut(s) 566, 915
BseSI GKGCMC 1 cut(s) 201
BseXI GCAGC 1 cut(s) 183
BshFI GGCC 3 cut(s) 101, 334, 492
BslFI GGGAC 1 cut(s) 375
BsmAI GTCTC 3 cut(s) 39, 414, 855
BsmFI GGGAC 1 cut(s) 375
BsnI GGCC 3 cut(s) 101, 334, 492
Bsp119I TTCGAA 1 cut(s) 887
Bsp1286I GDGCHC 1 cut(s) 201
Bsp143I GATC 3 cut(s) 370, 508, 713
BspACI CCGC 1 cut(s) 789
BspANI GGCC 3 cut(s) 101, 334, 492
BspHI TCATGA 1 cut(s) 585
BspLI GGNNCC 1 cut(s) 372
BspPI GGATC 4 cut(s) 365, 378, 503, 708
BspQI GCTCTTC 2 cut(s) 198, 645
BspT104I TTCGAA 1 cut(s) 887
BsrDI GCAATG 2 cut(s) 314, 406
BsrI ACTGG 1 cut(s) 331
BssECI CCNNGG 1 cut(s) 493
BssMI GATC 3 cut(s) 370, 508, 713
Bst2UI CCWGG 2 cut(s) 186, 495
Bst4CI ACNGT 3 cut(s) 50, 747, 811
Bst6I CTCTTC 2 cut(s) 198, 645
BstAPI GCANNNNNTGC 1 cut(s) 920
BstBI TTCGAA 1 cut(s) 887
BstC8I GCNNGC 2 cut(s) 490, 628
BstEII GGTNACC 1 cut(s) 56
BstF5I GGATG 3 cut(s) 546, 614, 615
BstKTI GATC 3 cut(s) 373, 511, 716
BstMAI GTCTC 3 cut(s) 39, 414, 855
BstMBI GATC 3 cut(s) 370, 508, 713
BstMWI GCNNNNNNNGC 4 cut(s) 205, 383, 485, 920
BstNI CCWGG 2 cut(s) 186, 495
BstNSI RCATGY 2 cut(s) 520, 898
BstPI GGTNACC 1 cut(s) 56
BstSCI CCNGG 2 cut(s) 184, 493
BstSLI GKGCMC 1 cut(s) 201
BstV1I GCAGC 1 cut(s) 183
BstV2I GAAGAC 1 cut(s) 329
BstX2I RGATCY 1 cut(s) 370
BstYI RGATCY 1 cut(s) 370
BsuRI GGCC 3 cut(s) 101, 334, 492
BtrI CACGTC 1 cut(s) 450
BtsCI GGATG 3 cut(s) 546, 614, 615
Cac8I GCNNGC 2 cut(s) 490, 628
CaiI CAGNNNCTG 1 cut(s) 494
CciI TCATGA 1 cut(s) 585
Cfr13I GGNCC 3 cut(s) 100, 491, 743
CseI GACGC 2 cut(s) 221, 880
Csp6I GTAC 3 cut(s) 253, 343, 556
CviAII CATG 7 cut(s) 269, 381, 517, 586, 816, 895, 929
CviQI GTAC 3 cut(s) 253, 343, 556
DpnI GATC 3 cut(s) 372, 510, 715
DpnII GATC 3 cut(s) 370, 508, 713
Eam1104I CTCTTC 2 cut(s) 198, 645
EarI CTCTTC 2 cut(s) 198, 645
Eco32I GATATC 1 cut(s) 296
Eco47I GGWCC 1 cut(s) 743
Eco57I CTGAAG 2 cut(s) 341, 626
Eco91I GGTNACC 1 cut(s) 56
EcoO109I RGGNCCY 2 cut(s) 100, 491
EcoO65I GGTNACC 1 cut(s) 56
EcoRII CCWGG 2 cut(s) 184, 493
EcoRV GATATC 1 cut(s) 296
EcoT22I ATGCAT 1 cut(s) 900
FaeI CATG 7 cut(s) 272, 384, 520, 589, 819, 898, 932
FalI AAGNNNNNCTT 2 cut(s) 672, 704
FaqI GGGAC 1 cut(s) 375
FatI CATG 7 cut(s) 268, 380, 516, 585, 815, 894, 928
FauI CCCGC 1 cut(s) 782
FauNDI CATATG 1 cut(s) 350
Fnu4HI GCNGC 1 cut(s) 172
FokI GGATG 3 cut(s) 553, 602, 621
Fsp4HI GCNGC 1 cut(s) 172
FspBI CTAG 4 cut(s) 137, 216, 728, 903
GluI GCNGC 1 cut(s) 172
HaeIII GGCC 3 cut(s) 101, 334, 492
HgaI GACGC 2 cut(s) 221, 880
Hin1II CATG 7 cut(s) 272, 384, 520, 589, 819, 898, 932
HincII GTYRAC 1 cut(s) 78
HindII GTYRAC 1 cut(s) 78
HinfI GANTC 5 cut(s) 93, 646, 683, 831, 952
Hpy166II GTNNAC 3 cut(s) 78, 118, 131
Hpy188I TCNGA 7 cut(s) 151, 293, 608, 645, 653, 860, 909
Hpy188III TCNNGA 6 cut(s) 90, 512, 586, 680, 717, 949
Hpy8I GTNNAC 3 cut(s) 78, 118, 131
Hpy99I CGWCG 1 cut(s) 454
HpyAV CCTTC 1 cut(s) 324
HpyCH4III ACNGT 3 cut(s) 50, 747, 811
HpyCH4IV ACGT 2 cut(s) 449, 845
HpyCH4V TGCA 7 cut(s) 171, 264, 314, 458, 488, 898, 914
HpyF10VI GCNNNNNNNGC 4 cut(s) 205, 383, 485, 920
HpySE526I ACGT 2 cut(s) 449, 845
Hsp92II CATG 7 cut(s) 272, 384, 520, 589, 819, 898, 932
Kzo9I GATC 3 cut(s) 370, 508, 713
LguI GCTCTTC 2 cut(s) 198, 645
LmnI GCTCC 3 cut(s) 598, 805, 928
Lsp1109I GCAGC 1 cut(s) 183
LweI GCATC 1 cut(s) 142
MaeI CTAG 4 cut(s) 137, 216, 728, 903
MaeII ACGT 2 cut(s) 449, 845
MaeIII GTNAC 4 cut(s) 56, 611, 755, 811
MalI GATC 3 cut(s) 372, 510, 715
MboI GATC 3 cut(s) 370, 508, 713
MboII GAAGA 4 cut(s) 215, 334, 632, 901
MflI RGATCY 1 cut(s) 370
MhlI GDGCHC 1 cut(s) 201
MluCI AATT 1 cut(s) 825
MlyI GAGTC 2 cut(s) 825, 946
MmeI TCCRAC 1 cut(s) 501
Mph1103I ATGCAT 1 cut(s) 900
MroXI GAANNNNTTC 1 cut(s) 639
MseI TTAA 2 cut(s) 675, 958
MspR9I CCNGG 2 cut(s) 186, 495
MvaI CCWGG 2 cut(s) 186, 495
MwoI GCNNNNNNNGC 4 cut(s) 205, 383, 485, 920
NdeI CATATG 1 cut(s) 350
NdeII GATC 3 cut(s) 370, 508, 713
NlaIII CATG 7 cut(s) 272, 384, 520, 589, 819, 898, 932
NlaIV GGNNCC 1 cut(s) 372
NmuCI GTSAC 2 cut(s) 611, 811
NsiI ATGCAT 1 cut(s) 900
NspI RCATGY 2 cut(s) 520, 898
NspV TTCGAA 1 cut(s) 887
PagI TCATGA 1 cut(s) 585
PciI ACATGT 1 cut(s) 516
PciSI GCTCTTC 2 cut(s) 198, 645
PcsI WCGNNNNNNNCGW 1 cut(s) 842
PdmI GAANNNNTTC 1 cut(s) 639
PfeI GAWTC 3 cut(s) 93, 646, 683
PkrI GCNGC 1 cut(s) 173
PleI GAGTC 2 cut(s) 825, 946
PpsI GAGTC 2 cut(s) 825, 946
PscI ACATGT 1 cut(s) 516
Psp6I CCWGG 2 cut(s) 184, 493
PspEI GGTNACC 1 cut(s) 56
PspGI CCWGG 2 cut(s) 184, 493
PspN4I GGNNCC 1 cut(s) 372
PspPI GGNCC 3 cut(s) 100, 491, 743
PstNI CAGNNNCTG 1 cut(s) 494
PsuI RGATCY 1 cut(s) 370
RsaI GTAC 3 cut(s) 254, 344, 557
RsaNI GTAC 3 cut(s) 253, 343, 556
SapI GCTCTTC 2 cut(s) 198, 645
SaqAI TTAA 2 cut(s) 675, 958
SatI GCNGC 1 cut(s) 172
Sau3AI GATC 3 cut(s) 370, 508, 713
Sau96I GGNCC 3 cut(s) 100, 491, 743
SchI GAGTC 2 cut(s) 825, 946
ScrFI CCNGG 2 cut(s) 186, 495
SduI GDGCHC 1 cut(s) 201
SfaNI GCATC 1 cut(s) 142
SfuI TTCGAA 1 cut(s) 887
SinI GGWCC 1 cut(s) 743
SpeI ACTAGT 1 cut(s) 902
Sse9I AATT 1 cut(s) 825
SsiI CCGC 1 cut(s) 789
SspMI CTAG 4 cut(s) 137, 216, 728, 903
StyD4I CCNGG 2 cut(s) 184, 493
TaaI ACNGT 3 cut(s) 50, 747, 811
TaiI ACGT 2 cut(s) 452, 848
TaqI TCGA 3 cut(s) 240, 452, 887
TasI AATT 1 cut(s) 825
TatI WGTACW 1 cut(s) 342
TfiI GAWTC 3 cut(s) 93, 646, 683
Tru1I TTAA 2 cut(s) 675, 958
Tru9I TTAA 2 cut(s) 675, 958
TseFI GTSAC 2 cut(s) 611, 811
TseI GCWGC 1 cut(s) 171
Tsp45I GTSAC 2 cut(s) 611, 811
TspDTI ATGAA 1 cut(s) 602
TspGWI ACGGA 1 cut(s) 549
VpaK11BI GGWCC 1 cut(s) 743
XceI RCATGY 2 cut(s) 520, 898
XmnI GAANNNNTTC 1 cut(s) 639
XspI CTAG 4 cut(s) 137, 216, 728, 903
Zsp2I ATGCAT 1 cut(s) 900
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.