MD05G1321500.v1.1

Alpha galactosidase A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0009821
Physical Location & Seq
Forward (+)
44865285 .. 44868145
2861 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1321500.v1.1.491

Sequence Viewer

Length: 1215 bp
ATGGATTGGAAAGGCAGGAGCGCACTCTTTGTTCAGCTCATTGTTGTCACATGTATTTTCAATGTGATTGATGCTCTGAACTACAAGAACAATACCCATCAAGCAGAATACGGTCAGTTCCTCATTGCCAATGGCGTTGCTCGTACACCTCCAATGGGTTGGAACAGTTGGAATCACTTCCACTGCAATATAAGTGAAGCCACAGTGAAGACCATTGCGGATGCTCTTGTTTCGACTGGCTTGGCAGCACTTGGATACAAATATGTCAATCTAGATGATTGTTGGGCTGAAAGAAACAGAGACAGTAGTGGTAACCTAATAGCAAAATCGTCAACTTTTCCTTCTGGAATCAAGGCCCTTGCTGATTACGTTCACGCAAGGGGTTTACAACTCGGCATATACTCTGATGCTGGTTATTATACTTGCAGCAACACAATGCCAGGCTCACTTGGGCACGAAGAGCAAGACGCTAGAACCTTTGCTGAATGGGGTGTCGATTATTTGAAGTACGATAACTGCAACCATGATGGTTCTAAACCTCAAATCAGATATCCTATAATGAGTTCTGCATTGCTAAAGACTGGAAGGCCAATCCTGTACTCCATATGCGAATGGGGACAAGAGGATCCTGCGACATGGGCTGGTCAGTATGGCAATGCTTGGAGAACTACGGGAGACATTGAGGACACTTGGGAAAGTATCACGTCGATTGCAGACCAAAACAACATTTGGGCTACTTATGCAGGCCCTGGCAAGTGGAATGATCCTGACATGTTGGAAGTGGGAAACGGAGGGATGAGTGTAGAGGAGTACCAATCTCATTTTAGCATTTGGGCAGTCATGAAAGCTCCTTTACTTATCGGATGTGACATCCCATCTGCGAGCAAGGAAGCTCTTCAGATTCTCGGAAACAAAGAGGTTATTGATGTTAATCAAGATTCACTTGGAGTTCAAGCGAGGAAACTGCGATCCAGAGATGACCTAGAAGTGTGGGCAGGACCGTTATCAAGTAACAGAGTGGTGGTAGTGTTGTGGAATAGAGGCGGGTCATTAGCTCCTATAACTGTGACATGGAAGGAAATTGGACTCTCGCCAAATACGTCTGTCATTGTCAGAGACTTATGGACGCATTCATTTGTTTCGAAGAACATGCATACTAGTCTGACTGCAAATGTTGCTCCTCATGGTTGTAATATGTATGTCCTGACTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 ko00561 ko00600 ko00603 map00052 map00561 map00600 map00603
Pfam Domains
Protein Families
Melibiase_2 Melibiase_2_C

Protein Analysis

405

Amino Acids

44.64

Weight (kDa)

5.47

Isoelectric Point (pI)

32.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Melibiase PF02065 47 - 135 1.6e-06 Melibiase
Melibiase_2 PF16499 49 - 313 1.8e-81 Alpha galactosidase A
Melibiase_2_C PF17450 316 - 396 9.6e-06 Alpha galactosidase A C-terminal beta sandwich domain
Melibiase_C PF17801 326 - 402 8.2e-20 Alpha galactosidase C-terminal beta sandwich domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 218, 1044
AclWI GGATC 4 cut(s) 620, 633, 758, 963
AcuI CTGAAG 1 cut(s) 881
AfaI GTAC 4 cut(s) 145, 509, 599, 812
AfiI CCNNNNNNNGG 1 cut(s) 155
AflIII ACRYGT 2 cut(s) 50, 771
AgsI TTSAA 3 cut(s) 61, 505, 953
AhlI ACTAGT 1 cut(s) 1157
AjiI CACGTC 1 cut(s) 705
AjnI CCWGG 2 cut(s) 439, 748
AluBI AGCT 4 cut(s) 37, 848, 893, 1055
AluI AGCT 4 cut(s) 37, 848, 893, 1055
Alw26I GTCTC 3 cut(s) 294, 669, 1110
AlwI GGATC 4 cut(s) 620, 633, 758, 963
AlwNI CAGNNNCTG 1 cut(s) 749
AoxI GGCC 3 cut(s) 354, 587, 745
ApeKI GCWGC 2 cut(s) 245, 426
Asp700I GAANNNNTTC 2 cut(s) 176, 894
AspLEI GCGC 1 cut(s) 23
AspS9I GGNCC 3 cut(s) 355, 746, 998
AsuII TTCGAA 1 cut(s) 1142
AvaII GGWCC 1 cut(s) 998
BaeGI GKGCMC 1 cut(s) 456
BamHI GGATCC 1 cut(s) 625
BbsI GAAGAC 1 cut(s) 215
BbvI GCAGC 2 cut(s) 257, 438
BccI CCATC 3 cut(s) 105, 521, 883
BcgI CGANNNNNNTGC 4 cut(s) 946, 980, 1132, 1166
BciT130I CCWGG 2 cut(s) 441, 750
BciVI GTATCC 1 cut(s) 248
BcoDI GTCTC 3 cut(s) 294, 669, 1110
BcuI ACTAGT 1 cut(s) 1157
BfaI CTAG 4 cut(s) 272, 471, 983, 1158
BfuI GTATCC 1 cut(s) 248
BisI GCNGC 2 cut(s) 246, 427
BlsI GCNGC 2 cut(s) 247, 428
Bme1390I CCNGG 2 cut(s) 441, 750
Bme18I GGWCC 1 cut(s) 998
BmgBI CACGTC 1 cut(s) 705
BmgT120I GGNCC 3 cut(s) 355, 746, 998
BmiI GGNNCC 1 cut(s) 627
BmrFI CCNGG 2 cut(s) 441, 750
BmsI GCATC 3 cut(s) 61, 211, 397
BpiI GAAGAC 1 cut(s) 215
Bpu14I TTCGAA 1 cut(s) 1142
BsaBI GATNNNNATC 1 cut(s) 930
BsaJI CCNNGG 1 cut(s) 748
Bsc4I CCNNNNNNNGG 1 cut(s) 155
Bse1I ACTGG 2 cut(s) 241, 586
Bse3DI GCAATG 4 cut(s) 123, 213, 569, 661
Bse8I GATNNNNATC 1 cut(s) 930
BseBI CCWGG 2 cut(s) 441, 750
BseDI CCNNGG 1 cut(s) 748
BseGI GGATG 4 cut(s) 226, 801, 869, 870
BseJI GATNNNNATC 1 cut(s) 930
BseLI CCNNNNNNNGG 1 cut(s) 155
BseMI GCAATG 4 cut(s) 123, 213, 569, 661
BseNI ACTGG 2 cut(s) 241, 586
BseRI GAGGAG 2 cut(s) 821, 1170
BseSI GKGCMC 1 cut(s) 456
BseXI GCAGC 2 cut(s) 257, 438
BshFI GGCC 3 cut(s) 356, 589, 747
BslFI GGGAC 1 cut(s) 630
BslI CCNNNNNNNGG 1 cut(s) 155
BsmAI GTCTC 3 cut(s) 294, 669, 1110
BsmFI GGGAC 1 cut(s) 630
BsmI GAATGC 1 cut(s) 1129
BsnI GGCC 3 cut(s) 356, 589, 747
Bsp119I TTCGAA 1 cut(s) 1142
Bsp1286I GDGCHC 1 cut(s) 456
Bsp143I GATC 3 cut(s) 625, 763, 968
BspACI CCGC 2 cut(s) 218, 1044
BspANI GGCC 3 cut(s) 356, 589, 747
BspHI TCATGA 1 cut(s) 840
BspLI GGNNCC 1 cut(s) 627
BspPI GGATC 4 cut(s) 620, 633, 758, 963
BspQI GCTCTTC 2 cut(s) 453, 900
BspT104I TTCGAA 1 cut(s) 1142
BsrDI GCAATG 4 cut(s) 123, 213, 569, 661
BsrI ACTGG 2 cut(s) 241, 586
BssECI CCNNGG 1 cut(s) 748
BssMI GATC 3 cut(s) 625, 763, 968
Bst2UI CCWGG 2 cut(s) 441, 750
Bst4CI ACNGT 6 cut(s) 113, 167, 205, 305, 1002, 1066
Bst6I CTCTTC 2 cut(s) 453, 900
BstAPI GCANNNNNTGC 1 cut(s) 1175
BstBI TTCGAA 1 cut(s) 1142
BstC8I GCNNGC 2 cut(s) 745, 883
BstEII GGTNACC 1 cut(s) 311
BstF5I GGATG 4 cut(s) 226, 801, 869, 870
BstHHI GCGC 1 cut(s) 23
BstKTI GATC 3 cut(s) 628, 766, 971
BstMAI GTCTC 3 cut(s) 294, 669, 1110
BstMBI GATC 3 cut(s) 625, 763, 968
BstMWI GCNNNNNNNGC 4 cut(s) 460, 638, 740, 1175
BstNI CCWGG 2 cut(s) 441, 750
BstNSI RCATGY 3 cut(s) 54, 775, 1153
BstPI GGTNACC 1 cut(s) 311
BstSCI CCNGG 2 cut(s) 439, 748
BstSLI GKGCMC 1 cut(s) 456
BstV1I GCAGC 2 cut(s) 257, 438
BstV2I GAAGAC 1 cut(s) 215
BstX2I RGATCY 1 cut(s) 625
BstXI CCANNNNNNTGG 1 cut(s) 159
BstYI RGATCY 1 cut(s) 625
BsuI GTATCC 1 cut(s) 248
BsuRI GGCC 3 cut(s) 356, 589, 747
BtrI CACGTC 1 cut(s) 705
BtsCI GGATG 4 cut(s) 226, 801, 869, 870
BtsI GCAGTG 1 cut(s) 181
BtsIMutI CAGTG 2 cut(s) 181, 210
Cac8I GCNNGC 2 cut(s) 745, 883
CaiI CAGNNNCTG 1 cut(s) 749
CciI TCATGA 1 cut(s) 840
CfoI GCGC 1 cut(s) 23
Cfr13I GGNCC 3 cut(s) 355, 746, 998
CseI GACGC 2 cut(s) 476, 1135
Csp6I GTAC 4 cut(s) 144, 508, 598, 811
CviAII CATG 8 cut(s) 51, 524, 636, 772, 841, 1071, 1150, 1184
CviQI GTAC 4 cut(s) 144, 508, 598, 811
DpnI GATC 3 cut(s) 627, 765, 970
DpnII GATC 3 cut(s) 625, 763, 968
Eam1104I CTCTTC 2 cut(s) 453, 900
EarI CTCTTC 2 cut(s) 453, 900
Eco32I GATATC 1 cut(s) 551
Eco47I GGWCC 1 cut(s) 998
Eco57I CTGAAG 1 cut(s) 881
Eco91I GGTNACC 1 cut(s) 311
EcoO109I RGGNCCY 2 cut(s) 355, 746
EcoO65I GGTNACC 1 cut(s) 311
EcoRII CCWGG 2 cut(s) 439, 748
EcoRV GATATC 1 cut(s) 551
EcoT22I ATGCAT 1 cut(s) 1155
FaeI CATG 8 cut(s) 54, 527, 639, 775, 844, 1074, 1153, 1187
FalI AAGNNNNNCTT 2 cut(s) 927, 959
FaqI GGGAC 1 cut(s) 630
FatI CATG 8 cut(s) 50, 523, 635, 771, 840, 1070, 1149, 1183
FauI CCCGC 1 cut(s) 1037
FauNDI CATATG 1 cut(s) 605
Fnu4HI GCNGC 2 cut(s) 246, 427
FokI GGATG 4 cut(s) 233, 808, 857, 876
Fsp4HI GCNGC 2 cut(s) 246, 427
FspBI CTAG 4 cut(s) 272, 471, 983, 1158
GlaI GCGC 1 cut(s) 22
GluI GCNGC 2 cut(s) 246, 427
HaeIII GGCC 3 cut(s) 356, 589, 747
HgaI GACGC 2 cut(s) 476, 1135
HhaI GCGC 1 cut(s) 23
Hin1II CATG 8 cut(s) 54, 527, 639, 775, 844, 1074, 1153, 1187
Hin6I GCGC 1 cut(s) 21
HinP1I GCGC 1 cut(s) 21
HincII GTYRAC 1 cut(s) 333
HindII GTYRAC 1 cut(s) 333
HinfI GANTC 6 cut(s) 172, 348, 901, 938, 1086, 1207
Hpy166II GTNNAC 4 cut(s) 146, 333, 373, 386
Hpy188I TCNGA 8 cut(s) 78, 406, 548, 863, 900, 908, 1115, 1164
Hpy188III TCNNGA 7 cut(s) 272, 345, 767, 841, 935, 972, 1204
Hpy8I GTNNAC 4 cut(s) 146, 333, 373, 386
Hpy99I CGWCG 1 cut(s) 709
HpyAV CCTTC 3 cut(s) 351, 579, 1069
HpyCH4III ACNGT 6 cut(s) 113, 167, 205, 305, 1002, 1066
HpyCH4IV ACGT 3 cut(s) 369, 704, 1100
HpyCH4V TGCA 8 cut(s) 186, 426, 519, 569, 713, 743, 1153, 1169
HpyF10VI GCNNNNNNNGC 4 cut(s) 460, 638, 740, 1175
HpySE526I ACGT 3 cut(s) 369, 704, 1100
Hsp92II CATG 8 cut(s) 54, 527, 639, 775, 844, 1074, 1153, 1187
HspAI GCGC 1 cut(s) 21
Kzo9I GATC 3 cut(s) 625, 763, 968
LguI GCTCTTC 2 cut(s) 453, 900
LmnI GCTCC 4 cut(s) 18, 853, 1060, 1183
Lsp1109I GCAGC 2 cut(s) 257, 438
LweI GCATC 3 cut(s) 61, 211, 397
MaeI CTAG 4 cut(s) 272, 471, 983, 1158
MaeII ACGT 3 cut(s) 369, 704, 1100
MaeIII GTNAC 5 cut(s) 46, 311, 866, 1010, 1066
MalI GATC 3 cut(s) 627, 765, 970
MboI GATC 3 cut(s) 625, 763, 968
MboII GAAGA 4 cut(s) 220, 470, 887, 1156
MflI RGATCY 1 cut(s) 625
MhlI GDGCHC 1 cut(s) 456
MluCI AATT 1 cut(s) 1080
MlyI GAGTC 2 cut(s) 1080, 1201
MmeI TCCRAC 3 cut(s) 140, 149, 756
Mph1103I ATGCAT 1 cut(s) 1155
MroXI GAANNNNTTC 2 cut(s) 176, 894
MseI TTAA 2 cut(s) 930, 1213
MspR9I CCNGG 2 cut(s) 441, 750
Mva1269I GAATGC 1 cut(s) 1129
MvaI CCWGG 2 cut(s) 441, 750
MwoI GCNNNNNNNGC 4 cut(s) 460, 638, 740, 1175
NdeI CATATG 1 cut(s) 605
NdeII GATC 3 cut(s) 625, 763, 968
NlaIII CATG 8 cut(s) 54, 527, 639, 775, 844, 1074, 1153, 1187
NlaIV GGNNCC 1 cut(s) 627
NmeAIII GCCGAG 1 cut(s) 372
NmuCI GTSAC 3 cut(s) 46, 866, 1066
NsiI ATGCAT 1 cut(s) 1155
NspI RCATGY 3 cut(s) 54, 775, 1153
NspV TTCGAA 1 cut(s) 1142
PagI TCATGA 1 cut(s) 840
PciI ACATGT 2 cut(s) 50, 771
PciSI GCTCTTC 2 cut(s) 453, 900
PcsI WCGNNNNNNNCGW 1 cut(s) 1097
PctI GAATGC 1 cut(s) 1129
PdmI GAANNNNTTC 2 cut(s) 176, 894
PfeI GAWTC 4 cut(s) 172, 348, 901, 938
PkrI GCNGC 2 cut(s) 247, 428
PleI GAGTC 2 cut(s) 1080, 1201
PpsI GAGTC 2 cut(s) 1080, 1201
PscI ACATGT 2 cut(s) 50, 771
Psp6I CCWGG 2 cut(s) 439, 748
PspEI GGTNACC 1 cut(s) 311
PspGI CCWGG 2 cut(s) 439, 748
PspN4I GGNNCC 1 cut(s) 627
PspPI GGNCC 3 cut(s) 355, 746, 998
PstNI CAGNNNCTG 1 cut(s) 749
PsuI RGATCY 1 cut(s) 625
RsaI GTAC 4 cut(s) 145, 509, 599, 812
RsaNI GTAC 4 cut(s) 144, 508, 598, 811
SapI GCTCTTC 2 cut(s) 453, 900
SaqAI TTAA 2 cut(s) 930, 1213
SatI GCNGC 2 cut(s) 246, 427
Sau3AI GATC 3 cut(s) 625, 763, 968
Sau96I GGNCC 3 cut(s) 355, 746, 998
SchI GAGTC 2 cut(s) 1080, 1201
ScrFI CCNGG 2 cut(s) 441, 750
SduI GDGCHC 1 cut(s) 456
SfaNI GCATC 3 cut(s) 61, 211, 397
SfuI TTCGAA 1 cut(s) 1142
SinI GGWCC 1 cut(s) 998
SpeI ACTAGT 1 cut(s) 1157
Sse9I AATT 1 cut(s) 1080
SsiI CCGC 2 cut(s) 218, 1044
SspMI CTAG 4 cut(s) 272, 471, 983, 1158
StyD4I CCNGG 2 cut(s) 439, 748
TaaI ACNGT 6 cut(s) 113, 167, 205, 305, 1002, 1066
TaiI ACGT 3 cut(s) 372, 707, 1103
TaqI TCGA 4 cut(s) 233, 495, 707, 1142
TasI AATT 1 cut(s) 1080
TatI WGTACW 1 cut(s) 597
TfiI GAWTC 4 cut(s) 172, 348, 901, 938
Tru1I TTAA 2 cut(s) 930, 1213
Tru9I TTAA 2 cut(s) 930, 1213
TscAI CASTG 2 cut(s) 188, 210
TseFI GTSAC 3 cut(s) 46, 866, 1066
TseI GCWGC 2 cut(s) 245, 426
Tsp45I GTSAC 3 cut(s) 46, 866, 1066
TspDTI ATGAA 2 cut(s) 857, 1122
TspGWI ACGGA 1 cut(s) 804
TspRI CASTG 2 cut(s) 188, 210
VpaK11BI GGWCC 1 cut(s) 998
XbaI TCTAGA 1 cut(s) 271
XceI RCATGY 3 cut(s) 54, 775, 1153
XmnI GAANNNNTTC 2 cut(s) 176, 894
XspI CTAG 4 cut(s) 272, 471, 983, 1158
Zsp2I ATGCAT 1 cut(s) 1155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.