Prupe.4G041000_v2.0.a1

Alpha galactosidase A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0009821
Physical Location & Seq
Reverse (-)
1931131 .. 1934502
3372 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G041000.2

Sequence Viewer

Length: 1212 bp
ATGGGTTGGAGAAACAGAAATTCTCTTCTCTTTTTTCTTGTTTTATTTTTTGTCCATGTGATAGATGCTATGAATTTCAGGAACAATACCCATCAAGCAGAATACAATCAGTTCCTTCTTGCCAATGGGGTTGCTCGAACACCTCCAATGGGTTGGAACAGTTGGAATCACTTCCAATGCAATATAAATGAAAGCACTGTGAAGACTATTGTGGATGCTCTTGTTTCAACTGGATTGGCAGCAGTTGGATACAAATATGTGAATTTAGATGATTGTTGGGCTGAAAAAAACAGAGACACGAGGGGTAATCTCAGAGCAAAATCATCTACTTTTCCTTCTGGAATCAAGACCCTTGCAGATTATGTTCATGCAAGAGGCTTAAAACTTGGCATATACTCTGATGCTGGCTATATGACCTGCAGCATGACAATGCCGGGCTCACTTGGGCATGAAGAGCAAGATGCAAGAACCTTCGCCGAGTGGGGGGTCGATTATTTGAAGTATGATAACTGTTACAATGATGGTTCTAAGCCTCAAAAAAGATATGCTAGAATGAGTTATGCACTGCAAAAGGTTGGAAGGCCGATTCTATACTCCTTATGCGAATGGGGACAAGAGAATCCTGCCAAATGGGCTGGTTTGTATGGCAATACTTGGAGAACTACAGGAGACATTAACGATACCTGGGCAAGTATCACGTCGATTGCAGATCAAAACAACATTTGGGGGAGATATGCAGGACCTGGCAAGTGGAATGATCCTGATATGTTGGAAGTGGGCAATGGAGGGATGAGTCTAGAGGAGTATAGATCTCATTTTAGCATTTGGGCAGTTATGAAAGCTCCTTTACTTATTGGATGTGACGTCCGATCTGCAAGCAAAGAGACTCTTCAGATCCTTGGAAACAAGGAGGTCATTAATGTTAATCAGGATCCAATGGGAGTTCAAGCAAGGAAATTGCGTTCAAAAGATGGCCTAGAAGTGTGGGCAGGACCATTATCAAGGAAAAGAGTGGTGGTAGTGTTGTGGAACAGAGGCAGGTCAGTTGCTCCTATATCTGTGACATGGAGGGACATTGGACTCTCTCCGTATAAGCCTGTTGTCGTCAGAGATCTGTGGGCGCACTCATTTGTTTCAAGGAACAAGCGTTTTCGGTTGACTGCAAATGTTGCTCCTCATGGTTGTAAGATGTATGCCCTAAGTCCCTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 ko00561 ko00600 ko00603 map00052 map00561 map00600 map00603
Pfam Domains
Protein Families
Melibiase_2 Melibiase_2_C

Protein Analysis

404

Amino Acids

45.56

Weight (kDa)

9.35

Isoelectric Point (pI)

33.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 867
Acc36I ACCTGC 2 cut(s) 425, 1029
AclWI GGATC 4 cut(s) 752, 889, 926, 939
AcsI RAATTY 3 cut(s) 19, 73, 262
AcuI CTGAAG 1 cut(s) 875
AcyI GRCGYC 1 cut(s) 864
AfiI CCNNNNNNNGG 2 cut(s) 149, 483
AgsI TTSAA 5 cut(s) 228, 499, 947, 966, 1137
AjiI CACGTC 1 cut(s) 699
AjnI CCWGG 2 cut(s) 683, 742
AloI GAACNNNNNNTCC 2 cut(s) 946, 978
AluBI AGCT 1 cut(s) 842
AluI AGCT 1 cut(s) 842
Alw26I GTCTC 3 cut(s) 288, 663, 878
AlwI GGATC 4 cut(s) 752, 889, 926, 939
AlwNI CAGNNNCTG 1 cut(s) 743
AoxI GGCC 2 cut(s) 581, 973
ApeKI GCWGC 2 cut(s) 239, 420
ApoI RAATTY 3 cut(s) 19, 73, 262
AseI ATTAAT 1 cut(s) 918
Asp700I GAANNNNTTC 1 cut(s) 170
AspLEI GCGC 1 cut(s) 1123
AspS9I GGNCC 2 cut(s) 740, 992
AsuC2I CCSGG 1 cut(s) 435
AvaII GGWCC 2 cut(s) 740, 992
BamHI GGATCC 1 cut(s) 931
BanII GRGCYC 1 cut(s) 440
BauI CACGAG 1 cut(s) 298
BbsI GAAGAC 1 cut(s) 209
BbvI GCAGC 2 cut(s) 251, 432
BccI CCATC 3 cut(s) 99, 515, 965
BciT130I CCWGG 2 cut(s) 685, 744
BciVI GTATCC 1 cut(s) 242
BcnI CCSGG 1 cut(s) 435
BcoDI GTCTC 3 cut(s) 288, 663, 878
BfaI CTAG 4 cut(s) 549, 797, 977, 1210
BfmI CTRYAG 2 cut(s) 418, 663
BfuAI ACCTGC 2 cut(s) 425, 1029
BfuI GTATCC 1 cut(s) 242
BglI GCCNNNNNGGC 1 cut(s) 632
BglII AGATCT 2 cut(s) 809, 1111
BisI GCNGC 2 cut(s) 240, 421
BlsI GCNGC 2 cut(s) 241, 422
Bme1390I CCNGG 3 cut(s) 435, 685, 744
Bme18I GGWCC 2 cut(s) 740, 992
BmgBI CACGTC 1 cut(s) 699
BmgT120I GGNCC 2 cut(s) 740, 992
BmiI GGNNCC 1 cut(s) 933
BmrFI CCNGG 3 cut(s) 435, 685, 744
BmsI GCATC 4 cut(s) 55, 205, 391, 451
BpiI GAAGAC 1 cut(s) 209
BpuMI CCSGG 1 cut(s) 435
BsaHI GRCGYC 1 cut(s) 864
BsaJI CCNNGG 2 cut(s) 684, 898
Bsc4I CCNNNNNNNGG 2 cut(s) 149, 483
Bse1I ACTGG 1 cut(s) 235
Bse3DI GCAATG 1 cut(s) 787
BseBI CCWGG 2 cut(s) 685, 744
BseDI CCNNGG 2 cut(s) 684, 898
BseGI GGATG 3 cut(s) 220, 795, 863
BseLI CCNNNNNNNGG 2 cut(s) 149, 483
BseMI GCAATG 1 cut(s) 787
BseMII CTCAG 1 cut(s) 325
BseNI ACTGG 1 cut(s) 235
BseRI GAGGAG 2 cut(s) 815, 1164
BseXI GCAGC 2 cut(s) 251, 432
BshFI GGCC 2 cut(s) 583, 975
BsiSI CCGG 1 cut(s) 434
BslFI GGGAC 3 cut(s) 624, 1085, 1188
BslI CCNNNNNNNGG 2 cut(s) 149, 483
BsmAI GTCTC 3 cut(s) 288, 663, 878
BsmFI GGGAC 3 cut(s) 624, 1085, 1188
BsnI GGCC 2 cut(s) 583, 975
Bsp1286I GDGCHC 1 cut(s) 440
Bsp143I GATC 7 cut(s) 709, 757, 809, 869, 894, 931, 1111
BspANI GGCC 2 cut(s) 583, 975
BspCNI CTCAG 1 cut(s) 324
BspLI GGNNCC 1 cut(s) 933
BspMAI CTGCAG 1 cut(s) 422
BspMI ACCTGC 2 cut(s) 425, 1029
BspPI GGATC 4 cut(s) 752, 889, 926, 939
BspQI GCTCTTC 1 cut(s) 447
BsrDI GCAATG 1 cut(s) 787
BsrI ACTGG 1 cut(s) 235
BssECI CCNNGG 2 cut(s) 684, 898
BssMI GATC 7 cut(s) 709, 757, 809, 869, 894, 931, 1111
BssNI GRCGYC 1 cut(s) 864
BssSI CACGAG 1 cut(s) 298
BssT1I CCWWGG 1 cut(s) 898
Bst2BI CACGAG 1 cut(s) 298
Bst2UI CCWGG 2 cut(s) 685, 744
Bst4CI ACNGT 3 cut(s) 161, 199, 512
Bst6I CTCTTC 3 cut(s) 30, 447, 894
BstACI GRCGYC 1 cut(s) 864
BstAPI GCANNNNNTGC 1 cut(s) 1169
BstC8I GCNNGC 2 cut(s) 406, 877
BstDEI CTNAG 3 cut(s) 311, 528, 1199
BstF5I GGATG 3 cut(s) 220, 795, 863
BstHHI GCGC 1 cut(s) 1123
BstKTI GATC 7 cut(s) 712, 760, 812, 872, 897, 934, 1114
BstMAI GTCTC 3 cut(s) 288, 663, 878
BstMBI GATC 7 cut(s) 709, 757, 809, 869, 894, 931, 1111
BstMWI GCNNNNNNNGC 3 cut(s) 454, 632, 1169
BstNI CCWGG 2 cut(s) 685, 744
BstSCI CCNGG 3 cut(s) 433, 683, 742
BstSFI CTRYAG 2 cut(s) 418, 663
BstV1I GCAGC 2 cut(s) 251, 432
BstV2I GAAGAC 1 cut(s) 209
BstX2I RGATCY 4 cut(s) 809, 894, 931, 1111
BstXI CCANNNNNNTGG 1 cut(s) 153
BstYI RGATCY 4 cut(s) 809, 894, 931, 1111
BsuI GTATCC 1 cut(s) 242
BsuRI GGCC 2 cut(s) 583, 975
BtrI CACGTC 1 cut(s) 699
BtsCI GGATG 3 cut(s) 220, 795, 863
BtsI GCAGTG 1 cut(s) 563
BtsIMutI CAGTG 2 cut(s) 195, 563
BveI ACCTGC 2 cut(s) 425, 1029
Cac8I GCNNGC 2 cut(s) 406, 877
CaiI CAGNNNCTG 1 cut(s) 743
CfoI GCGC 1 cut(s) 1123
Cfr13I GGNCC 2 cut(s) 740, 992
CviAII CATG 6 cut(s) 56, 368, 424, 449, 1065, 1178
DdeI CTNAG 3 cut(s) 311, 528, 1199
DpnI GATC 7 cut(s) 711, 759, 811, 871, 896, 933, 1113
DpnII GATC 7 cut(s) 709, 757, 809, 869, 894, 931, 1111
Eam1104I CTCTTC 3 cut(s) 30, 447, 894
EarI CTCTTC 3 cut(s) 30, 447, 894
Eco130I CCWWGG 1 cut(s) 898
Eco24I GRGCYC 1 cut(s) 440
Eco47I GGWCC 2 cut(s) 740, 992
Eco57I CTGAAG 1 cut(s) 875
EcoO109I RGGNCCY 1 cut(s) 740
EcoRII CCWGG 2 cut(s) 683, 742
EcoT14I CCWWGG 1 cut(s) 898
EcoT38I GRGCYC 1 cut(s) 440
ErhI CCWWGG 1 cut(s) 898
FaeI CATG 6 cut(s) 59, 371, 427, 452, 1068, 1181
FalI AAGNNNNNCTT 2 cut(s) 873, 905
FaqI GGGAC 3 cut(s) 624, 1085, 1188
FatI CATG 6 cut(s) 55, 367, 423, 448, 1064, 1177
Fnu4HI GCNGC 2 cut(s) 240, 421
FokI GGATG 3 cut(s) 227, 802, 870
FriOI GRGCYC 1 cut(s) 440
Fsp4HI GCNGC 2 cut(s) 240, 421
FspBI CTAG 4 cut(s) 549, 797, 977, 1210
GlaI GCGC 1 cut(s) 1122
GluI GCNGC 2 cut(s) 240, 421
HaeIII GGCC 2 cut(s) 583, 975
HapII CCGG 1 cut(s) 434
HhaI GCGC 1 cut(s) 1123
Hin1I GRCGYC 1 cut(s) 864
Hin1II CATG 6 cut(s) 59, 371, 427, 452, 1068, 1181
Hin6I GCGC 1 cut(s) 1121
HinP1I GCGC 1 cut(s) 1121
HincII GTYRAC 1 cut(s) 1158
HindII GTYRAC 1 cut(s) 1158
HinfI GANTC 7 cut(s) 166, 342, 586, 619, 793, 886, 1080
HpaII CCGG 1 cut(s) 434
Hpy166II GTNNAC 1 cut(s) 1158
Hpy188I TCNGA 5 cut(s) 314, 400, 869, 894, 1109
Hpy188III TCNNGA 6 cut(s) 79, 339, 346, 761, 797, 929
Hpy8I GTNNAC 1 cut(s) 1158
Hpy99I CGWCG 1 cut(s) 703
HpyAV CCTTC 4 cut(s) 125, 345, 481, 573
HpyCH4III ACNGT 3 cut(s) 161, 199, 512
HpyCH4IV ACGT 2 cut(s) 698, 864
HpyF10VI GCNNNNNNNGC 3 cut(s) 454, 632, 1169
HpyF3I CTNAG 3 cut(s) 311, 528, 1199
HpySE526I ACGT 2 cut(s) 698, 864
Hsp92I GRCGYC 1 cut(s) 864
Hsp92II CATG 6 cut(s) 59, 371, 427, 452, 1068, 1181
HspAI GCGC 1 cut(s) 1121
Kzo9I GATC 7 cut(s) 709, 757, 809, 869, 894, 931, 1111
LguI GCTCTTC 1 cut(s) 447
LmnI GCTCC 3 cut(s) 847, 1054, 1177
Lsp1109I GCAGC 2 cut(s) 251, 432
LweI GCATC 4 cut(s) 55, 205, 391, 451
MaeI CTAG 4 cut(s) 549, 797, 977, 1210
MaeII ACGT 2 cut(s) 698, 864
MaeIII GTNAC 3 cut(s) 512, 860, 1060
MalI GATC 7 cut(s) 711, 759, 811, 871, 896, 933, 1113
MboI GATC 7 cut(s) 709, 757, 809, 869, 894, 931, 1111
MboII GAAGA 4 cut(s) 17, 214, 464, 881
MflI RGATCY 4 cut(s) 809, 894, 931, 1111
MhlI GDGCHC 1 cut(s) 440
MluCI AATT 4 cut(s) 19, 73, 262, 956
MlyI GAGTC 3 cut(s) 802, 880, 1074
MmeI TCCRAC 5 cut(s) 134, 143, 226, 556, 750
MroXI GAANNNNTTC 1 cut(s) 170
MseI TTAA 4 cut(s) 380, 675, 918, 924
MslI CAYNNNNRTG 1 cut(s) 428
MspI CCGG 1 cut(s) 434
MspR9I CCNGG 3 cut(s) 435, 685, 744
MvaI CCWGG 2 cut(s) 685, 744
MwoI GCNNNNNNNGC 3 cut(s) 454, 632, 1169
NciI CCSGG 1 cut(s) 435
NdeII GATC 7 cut(s) 709, 757, 809, 869, 894, 931, 1111
NlaIII CATG 6 cut(s) 59, 371, 427, 452, 1068, 1181
NlaIV GGNNCC 1 cut(s) 933
NmeAIII GCCGAG 1 cut(s) 502
NmuCI GTSAC 2 cut(s) 860, 1060
PciSI GCTCTTC 1 cut(s) 447
PdmI GAANNNNTTC 1 cut(s) 170
PfeI GAWTC 4 cut(s) 166, 342, 586, 619
PkrI GCNGC 2 cut(s) 241, 422
PleI GAGTC 3 cut(s) 801, 880, 1074
PpsI GAGTC 3 cut(s) 801, 880, 1074
PpuMI RGGWCCY 1 cut(s) 740
PshBI ATTAAT 1 cut(s) 918
Psp5II RGGWCCY 1 cut(s) 740
Psp6I CCWGG 2 cut(s) 683, 742
PspGI CCWGG 2 cut(s) 683, 742
PspN4I GGNNCC 1 cut(s) 933
PspPI GGNCC 2 cut(s) 740, 992
PspPPI RGGWCCY 1 cut(s) 740
PstI CTGCAG 1 cut(s) 422
PstNI CAGNNNCTG 1 cut(s) 743
PsuI RGATCY 4 cut(s) 809, 894, 931, 1111
RseI CAYNNNNRTG 1 cut(s) 428
SapI GCTCTTC 1 cut(s) 447
SaqAI TTAA 4 cut(s) 380, 675, 918, 924
SatI GCNGC 2 cut(s) 240, 421
Sau3AI GATC 7 cut(s) 709, 757, 809, 869, 894, 931, 1111
Sau96I GGNCC 2 cut(s) 740, 992
SchI GAGTC 3 cut(s) 802, 880, 1074
ScrFI CCNGG 3 cut(s) 435, 685, 744
SduI GDGCHC 1 cut(s) 440
SfaNI GCATC 4 cut(s) 55, 205, 391, 451
SfcI CTRYAG 2 cut(s) 418, 663
SinI GGWCC 2 cut(s) 740, 992
SmiMI CAYNNNNRTG 1 cut(s) 428
Sse9I AATT 4 cut(s) 19, 73, 262, 956
SspMI CTAG 4 cut(s) 549, 797, 977, 1210
StyD4I CCNGG 3 cut(s) 433, 683, 742
StyI CCWWGG 1 cut(s) 898
TaaI ACNGT 3 cut(s) 161, 199, 512
TaiI ACGT 2 cut(s) 701, 867
TaqI TCGA 3 cut(s) 136, 489, 701
TasI AATT 4 cut(s) 19, 73, 262, 956
TfiI GAWTC 4 cut(s) 166, 342, 586, 619
Tru1I TTAA 4 cut(s) 380, 675, 918, 924
Tru9I TTAA 4 cut(s) 380, 675, 918, 924
TscAI CASTG 2 cut(s) 202, 570
TseFI GTSAC 2 cut(s) 860, 1060
TseI GCWGC 2 cut(s) 239, 420
Tsp45I GTSAC 2 cut(s) 860, 1060
TspDTI ATGAA 5 cut(s) 86, 204, 356, 465, 851
TspGWI ACGGA 1 cut(s) 1077
TspRI CASTG 2 cut(s) 202, 570
VpaK11BI GGWCC 2 cut(s) 740, 992
VspI ATTAAT 1 cut(s) 918
XapI RAATTY 3 cut(s) 19, 73, 262
XbaI TCTAGA 1 cut(s) 796
XmnI GAANNNNTTC 1 cut(s) 170
XspI CTAG 4 cut(s) 549, 797, 977, 1210
ZraI GACGTC 1 cut(s) 865
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.