Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0009821

Physical Location & Seq

Genomic Coordinates Reverse (-)

12843588 .. 12849729

Gene Structure 6142 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G006260.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1251 bp

ATGTGGAAATGCAAATTAACTTTACCACTATTTTGTATCTTTATGCGAGCAGCTTTGGTGGCGATCTGCATTTCTTTTGTGTTCAATACCAACTGCGCAGTTGCTGGTCGGCAGATAATAAGATCCAGTACTGGACAGGATCATATTAGGAGGAACCTCTTGGACAATGGACTAGGTATAACACCACCTATGGGATGGAATAGTTGGAATCACTTCAACTGTCACATTGAAGAAAAATTGATTAGAGAAACTGCGGACTCGATGGTATCAACAGGACTTGCTGCACTTGGCTACGAATATATAAATTTAGATGATTGCTGGGCTGAACTTGATCGAGATTCGGAGGGAAATTTCGTTCCTAAATCTTCAGCATTTCCATCTGGAATCAAGGCTTTAGCAGATTATGTCCACAGCAAAGGGCTGAAGCTTGGGATTTACTCTGATGCTGGAACTCTGACATGCAGTAAACAAATGCCCGGATCACTAGGCCATGAGGAACAAGATGCACAAACTTTCGCTTCCTGGGGAATTGACTACTTGAAGTATGATAATTGTCACAATACGGGTACAAGTCCTAAAGAAAGGTATCCAGTAATGAGTAAAGCTTTACTAAACTCCACAAGGCCCATCTTCTTTTCCCTATGTGAATGGGGACAGGAAGACCCTGCAACTTGGGCAGCGAGTGTTGGAAATAGTTGGAGAACAACAGGAGACATTGGTGATAACTGGAATAGTATGACATCTCGCGCGGATCAAAACGACAAATGGGCATCATACGCTGGGCCTGGAGGATGGAATGATCCTGATATGCTTGAAGTTGGAAATGGAGGTATGACTGAGGAGGAATATCGTGCACATTTCAGCATCTGGGCATTAGCTAAGGCTCCTCTGTTAATTGGATGTGATATACGATCTATGGACAATGCGACTCTCAATCTGTTAAGCAACAAGGAAGTTATTGCAGTTAATCAAGACAAACTTGGAGAACAAGGGAAACAAGTTACAAGTGGTACGGATTTGCAGGTATGGGCTGGCCCTCTTAGTGATAACAGGGTAGCATTAGTACTGTGGAACAGAGGTCCTTCAAAAGCTACGGTCACTGCTCATTGGTCCGATATAGGCCTTGAACCAACAACCGTTGTCAAACCAAGAGATTTATGGGAGCATTCAACTGGATCATCAGTCAGTGAAACACTCTCTGCTGATCTCGAGTCTCATGCTTGCAAAATGTACGTACTAAGTTCCAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004557 GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0007275 GO:0008150 GO:0009505 GO:0009605 GO:0009607 GO:0009620 GO:0009653 GO:0009909 GO:0009911 GO:0009965 GO:0010016 GO:0015925 GO:0016787 GO:0016798 GO:0030312 GO:0032501 GO:0032502 GO:0043207 GO:0044464 GO:0048046 GO:0048366 GO:0048367 GO:0048518 GO:0048580 GO:0048582 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050896 GO:0051094 GO:0051239 GO:0051240 GO:0051704 GO:0051707 GO:0065007 GO:0071944 GO:0099402 GO:1905392 GO:2000026 GO:2000241 GO:2000243

KEGG Pathways

Metabolic & Signaling

ko00052 ko00561 ko00600 ko00603 map00052 map00561 map00600 map00603

Pfam Domains

Protein Families

Melibiase_2 Melibiase_2_C

Protein Analysis

416

Amino Acids

45.8

Weight (kDa)

5.33

Isoelectric Point (pI)

34.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Melibiase_2	PF16499	61 - 325	7e-79	Alpha galactosidase A
Melibiase_C	PF17801	338 - 413	5.5e-18	Alpha galactosidase C-terminal beta sandwich domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009821)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G08370 AT5G08370
fragaria_vesca	FvH4_4g05940 FvH4_4g05940 FvH4_4g05940
malus_domestica	MD05G1321500.v1.1 MD05G1321600.v1.1 MD05G1321700.v1.1
prunus_persica	Prupe.4G041000_v2.0.a1 Prupe.4G041000_v2.0.a1
pyrus_communis	pycom05g29600 pycom05g29610
rosa_chinensis	RchiOBHm_Chr4g0397131
rosa_laevigata	RLG00000009444
rosa_multiflora	Rmu_sc0002312.1_g000033
rosa_roxburghii	Rroxscaffold_5G00342100
rosa_rugosa	Rorug03G0344600
rosa_samantha	Rh4AG077500 Rh4CG082700 Rh4DG070600
rosa_wichuraiana	Rw4G006260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	97
Acc36I	ACCTGC	1 cut(s)	1012
AccII	CGCG	2 cut(s)	747, 749
AciI	CCGC	2 cut(s)	254, 749
AclWI	GGATC	6 cut(s)	117, 147, 487, 759, 794, 1183
AcsI	RAATTY	2 cut(s)	304, 349
AcuI	CTGAAG	2 cut(s)	351, 443
AfaI	GTAC	6 cut(s)	130, 568, 1012, 1065, 1232, 1236
AfiI	CCNNNNNNNGG	1 cut(s)	191
AgsI	TTSAA	8 cut(s)	85, 217, 230, 541, 815, 1086, 1127, 1170
AjnI	CCWGG	2 cut(s)	521, 784
AloI	GAACNNNNNNTCC	2 cut(s)	339, 371
AluBI	AGCT	5 cut(s)	53, 427, 605, 878, 1091
AluI	AGCT	5 cut(s)	53, 427, 605, 878, 1091
Alw21I	GWGCWC	1 cut(s)	856
Alw26I	GTCTC	2 cut(s)	705, 1218
Alw44I	GTGCAC	1 cut(s)	852
AlwI	GGATC	6 cut(s)	117, 147, 487, 759, 794, 1183
AlwNI	CAGNNNCTG	2 cut(s)	104, 867
Ama87I	CYCGRG	1 cut(s)	1208
AoxI	GGCC	5 cut(s)	487, 623, 782, 1033, 1120
ApaLI	GTGCAC	1 cut(s)	852
ApeKI	GCWGC	3 cut(s)	50, 281, 677
ApoI	RAATTY	2 cut(s)	304, 349
Asp700I	GAANNNNTTC	1 cut(s)	212
AspLEI	GCGC	2 cut(s)	98, 749
AspS9I	GGNCC	5 cut(s)	624, 782, 1034, 1079, 1110
AsuC2I	CCSGG	1 cut(s)	477
AsuHPI	GGTGA	1 cut(s)	731
AvaI	CYCGRG	1 cut(s)	1208
AvaII	GGWCC	2 cut(s)	1079, 1110
BaeGI	GKGCMC	1 cut(s)	856
BbsI	GAAGAC	1 cut(s)	666
Bbv12I	GWGCWC	1 cut(s)	856
BbvI	GCAGC	3 cut(s)	62, 268, 689
BccI	CCATC	5 cut(s)	189, 256, 385, 635, 786
BcgI	CGANNNNNNTGC	2 cut(s)	1199, 1233
BciT130I	CCWGG	2 cut(s)	523, 786
BciVI	GTATCC	1 cut(s)	597
BcnI	CCSGG	1 cut(s)	477
BcoDI	GTCTC	2 cut(s)	705, 1218
BfaI	CTAG	2 cut(s)	173, 485
BfuAI	ACCTGC	1 cut(s)	1012
BfuI	GTATCC	1 cut(s)	597
BisI	GCNGC	3 cut(s)	51, 282, 678
BlsI	GCNGC	3 cut(s)	52, 283, 679
BmcAI	AGTACT	2 cut(s)	130, 1065
Bme1390I	CCNGG	3 cut(s)	477, 523, 786
Bme18I	GGWCC	2 cut(s)	1079, 1110
BmeT110I	CYCGRG	1 cut(s)	1208
BmgT120I	GGNCC	5 cut(s)	624, 782, 1034, 1079, 1110
BmiI	GGNNCC	2 cut(s)	155, 885
BmrFI	CCNGG	3 cut(s)	477, 523, 786
BmsI	GCATC	4 cut(s)	433, 493, 779, 873
BpiI	GAAGAC	1 cut(s)	666
BpmI	CTGGAG	1 cut(s)	807
Bpu10I	CCTNAGC	1 cut(s)	879
BpuMI	CCSGG	1 cut(s)	477
BsaAI	YACGTR	1 cut(s)	1234
BsaJI	CCNNGG	1 cut(s)	522
Bsc4I	CCNNNNNNNGG	1 cut(s)	191
Bse1I	ACTGG	5 cut(s)	126, 136, 590, 731, 1177
BseBI	CCWGG	2 cut(s)	523, 786
BseDI	CCNNGG	1 cut(s)	522
BseGI	GGATG	3 cut(s)	200, 797, 905
BseLI	CCNNNNNNNGG	1 cut(s)	191
BseMII	CTCAG	1 cut(s)	828
BseNI	ACTGG	5 cut(s)	126, 136, 590, 731, 1177
BseRI	GAGGAG	2 cut(s)	854, 876
BseSI	GKGCMC	1 cut(s)	856
BseXI	GCAGC	3 cut(s)	62, 268, 689
BseYI	CCCAGC	2 cut(s)	318, 779
BsgI	GTGCAG	1 cut(s)	267
Bsh1236I	CGCG	2 cut(s)	747, 749
BshFI	GGCC	5 cut(s)	489, 625, 784, 1035, 1122
BsiHKAI	GWGCWC	1 cut(s)	856
BsiHKCI	CYCGRG	1 cut(s)	1208
BsiSI	CCGG	1 cut(s)	477
BslFI	GGGAC	1 cut(s)	666
BslI	CCNNNNNNNGG	1 cut(s)	191
BsmAI	GTCTC	2 cut(s)	705, 1218
BsmFI	GGGAC	1 cut(s)	666
BsmI	GAATGC	1 cut(s)	1165
BsnI	GGCC	5 cut(s)	489, 625, 784, 1035, 1122
BsoBI	CYCGRG	1 cut(s)	1208
Bsp1286I	GDGCHC	1 cut(s)	856
BspACI	CCGC	2 cut(s)	254, 749
BspANI	GGCC	5 cut(s)	489, 625, 784, 1035, 1122
BspCNI	CTCAG	1 cut(s)	829
BspFNI	CGCG	2 cut(s)	747, 749
BspLI	GGNNCC	2 cut(s)	155, 885
BspMI	ACCTGC	1 cut(s)	1012
BspPI	GGATC	6 cut(s)	117, 147, 487, 759, 794, 1183
BsrI	ACTGG	5 cut(s)	126, 136, 590, 731, 1177
BssECI	CCNNGG	1 cut(s)	522
Bst2UI	CCWGG	2 cut(s)	523, 786
Bst4CI	ACNGT	4 cut(s)	221, 1068, 1096, 1138
BstBAI	YACGTR	1 cut(s)	1234
BstC8I	GCNNGC	3 cut(s)	48, 1033, 1222
BstDEI	CTNAG	4 cut(s)	837, 879, 1040, 1238
BstF5I	GGATG	3 cut(s)	200, 797, 905
BstFNI	CGCG	2 cut(s)	747, 749
BstHHI	GCGC	2 cut(s)	98, 749
BstMAI	GTCTC	2 cut(s)	705, 1218
BstMWI	GCNNNNNNNGC	3 cut(s)	59, 674, 776
BstNI	CCWGG	2 cut(s)	523, 786
BstNSI	RCATGY	1 cut(s)	462
BstSCI	CCNGG	3 cut(s)	475, 521, 784
BstSLI	GKGCMC	1 cut(s)	856
BstSNI	TACGTA	1 cut(s)	1234
BstUI	CGCG	2 cut(s)	747, 749
BstV1I	GCAGC	3 cut(s)	62, 268, 689
BstV2I	GAAGAC	1 cut(s)	666
BstX2I	RGATCY	1 cut(s)	122
BstYI	RGATCY	1 cut(s)	122
BsuI	GTATCC	1 cut(s)	597
BsuRI	GGCC	5 cut(s)	489, 625, 784, 1035, 1122
BtsCI	GGATG	3 cut(s)	200, 797, 905
BtsI	GCAGTG	1 cut(s)	1098
BtsIMutI	CAGTG	2 cut(s)	1098, 1192
BveI	ACCTGC	1 cut(s)	1012
Cac8I	GCNNGC	3 cut(s)	48, 1033, 1222
CaiI	CAGNNNCTG	2 cut(s)	104, 867
CfoI	GCGC	2 cut(s)	98, 749
Cfr13I	GGNCC	5 cut(s)	624, 782, 1034, 1079, 1110
Csp6I	GTAC	6 cut(s)	129, 567, 1011, 1064, 1231, 1235
CviAII	CATG	3 cut(s)	459, 491, 1217
CviQI	GTAC	6 cut(s)	129, 567, 1011, 1064, 1231, 1235
DdeI	CTNAG	4 cut(s)	837, 879, 1040, 1238
Eco105I	TACGTA	1 cut(s)	1234
Eco147I	AGGCCT	1 cut(s)	1122
Eco47I	GGWCC	2 cut(s)	1079, 1110
Eco57I	CTGAAG	2 cut(s)	351, 443
Eco88I	CYCGRG	1 cut(s)	1208
EcoO109I	RGGNCCY	1 cut(s)	1079
EcoRII	CCWGG	2 cut(s)	521, 784
FaeI	CATG	3 cut(s)	462, 494, 1220
FalI	AAGNNNNNCTT	2 cut(s)	963, 995
FaqI	GGGAC	1 cut(s)	666
FatI	CATG	3 cut(s)	458, 490, 1216
Fnu4HI	GCNGC	3 cut(s)	51, 282, 678
FokI	GGATG	3 cut(s)	207, 804, 912
Fsp4HI	GCNGC	3 cut(s)	51, 282, 678
FspBI	CTAG	2 cut(s)	173, 485
FspI	TGCGCA	1 cut(s)	97
GlaI	GCGC	2 cut(s)	97, 748
GluI	GCNGC	3 cut(s)	51, 282, 678
GsaI	CCCAGC	2 cut(s)	322, 783
GsuI	CTGGAG	1 cut(s)	807
HaeIII	GGCC	5 cut(s)	489, 625, 784, 1035, 1122
HapII	CCGG	1 cut(s)	477
HhaI	GCGC	2 cut(s)	98, 749
Hin1II	CATG	3 cut(s)	462, 494, 1220
Hin6I	GCGC	2 cut(s)	96, 747
HinP1I	GCGC	2 cut(s)	96, 747
HindIII	AAGCTT	2 cut(s)	425, 603
HinfI	GANTC	6 cut(s)	208, 257, 338, 384, 928, 1211
HpaII	CCGG	1 cut(s)	477
HphI	GGTGA	1 cut(s)	731
Hpy166II	GTNNAC	3 cut(s)	409, 467, 854
Hpy188I	TCNGA	4 cut(s)	343, 442, 456, 1114
Hpy188III	TCNNGA	5 cut(s)	335, 381, 803, 971, 1208
Hpy8I	GTNNAC	3 cut(s)	409, 467, 854
HpyAV	CCTTC	1 cut(s)	1092
HpyCH4III	ACNGT	4 cut(s)	221, 1068, 1096, 1138
HpyCH4IV	ACGT	1 cut(s)	1233
HpyF10VI	GCNNNNNNNGC	3 cut(s)	59, 674, 776
HpyF3I	CTNAG	4 cut(s)	837, 879, 1040, 1238
HpySE526I	ACGT	1 cut(s)	1233
Hsp92II	CATG	3 cut(s)	462, 494, 1220
HspAI	GCGC	2 cut(s)	96, 747
LmnI	GCTCC	2 cut(s)	889, 1162
Lsp1109I	GCAGC	3 cut(s)	62, 268, 689
LweI	GCATC	4 cut(s)	433, 493, 779, 873
MaeI	CTAG	2 cut(s)	173, 485
MaeII	ACGT	1 cut(s)	1233
MaeIII	GTNAC	4 cut(s)	221, 554, 1000, 1096
MboII	GAAGA	4 cut(s)	242, 357, 622, 671
MflI	RGATCY	1 cut(s)	122
MhlI	GDGCHC	1 cut(s)	856
MluCI	AATT	7 cut(s)	14, 236, 304, 349, 528, 550, 894
MlyI	GAGTC	3 cut(s)	251, 922, 1220
MmeI	TCCRAC	4 cut(s)	185, 667, 677, 799
MroXI	GAANNNNTTC	1 cut(s)	212
MseI	TTAA	4 cut(s)	17, 893, 941, 966
MspI	CCGG	1 cut(s)	477
MspR9I	CCNGG	3 cut(s)	477, 523, 786
Mva1269I	GAATGC	1 cut(s)	1165
MvaI	CCWGG	2 cut(s)	523, 786
MvnI	CGCG	2 cut(s)	747, 749
MwoI	GCNNNNNNNGC	3 cut(s)	59, 674, 776
NciI	CCSGG	1 cut(s)	477
NlaIII	CATG	3 cut(s)	462, 494, 1220
NlaIV	GGNNCC	2 cut(s)	155, 885
NmuCI	GTSAC	3 cut(s)	221, 554, 1096
NsbI	TGCGCA	1 cut(s)	97
NspI	RCATGY	1 cut(s)	462
PaeR7I	CTCGAG	1 cut(s)	1208
PceI	AGGCCT	1 cut(s)	1122
PctI	GAATGC	1 cut(s)	1165
PdmI	GAANNNNTTC	1 cut(s)	212
PfeI	GAWTC	3 cut(s)	208, 338, 384
PkrI	GCNGC	3 cut(s)	52, 283, 679
PleI	GAGTC	3 cut(s)	251, 922, 1219
PpsI	GAGTC	3 cut(s)	251, 922, 1219
Ppu21I	YACGTR	1 cut(s)	1234
PpuMI	RGGWCCY	1 cut(s)	1079
Psp5II	RGGWCCY	1 cut(s)	1079
Psp6I	CCWGG	2 cut(s)	521, 784
PspFI	CCCAGC	2 cut(s)	318, 779
PspGI	CCWGG	2 cut(s)	521, 784
PspN4I	GGNNCC	2 cut(s)	155, 885
PspPI	GGNCC	5 cut(s)	624, 782, 1034, 1079, 1110
PspPPI	RGGWCCY	1 cut(s)	1079
PstNI	CAGNNNCTG	2 cut(s)	104, 867
PsuI	RGATCY	1 cut(s)	122
RsaI	GTAC	6 cut(s)	130, 568, 1012, 1065, 1232, 1236
RsaNI	GTAC	6 cut(s)	129, 567, 1011, 1064, 1231, 1235
SaqAI	TTAA	4 cut(s)	17, 893, 941, 966
SatI	GCNGC	3 cut(s)	51, 282, 678
Sau96I	GGNCC	5 cut(s)	624, 782, 1034, 1079, 1110
ScaI	AGTACT	2 cut(s)	130, 1065
SchI	GAGTC	3 cut(s)	251, 922, 1220
ScrFI	CCNGG	3 cut(s)	477, 523, 786
SduI	GDGCHC	1 cut(s)	856
SfaNI	GCATC	4 cut(s)	433, 493, 779, 873
Sfr274I	CTCGAG	1 cut(s)	1208
SinI	GGWCC	2 cut(s)	1079, 1110
SlaI	CTCGAG	1 cut(s)	1208
SmlI	CTYRAG	1 cut(s)	1208
SmoI	CTYRAG	1 cut(s)	1208
SnaBI	TACGTA	1 cut(s)	1234
Sse9I	AATT	7 cut(s)	14, 236, 304, 349, 528, 550, 894
SseBI	AGGCCT	1 cut(s)	1122
SsiI	CCGC	2 cut(s)	254, 749
SspMI	CTAG	2 cut(s)	173, 485
StuI	AGGCCT	1 cut(s)	1122
StyD4I	CCNGG	3 cut(s)	475, 521, 784
TaaI	ACNGT	4 cut(s)	221, 1068, 1096, 1138
TaiI	ACGT	1 cut(s)	1236
TaqI	TCGA	3 cut(s)	260, 334, 1209
TasI	AATT	7 cut(s)	14, 236, 304, 349, 528, 550, 894
TatI	WGTACW	2 cut(s)	128, 1063
TfiI	GAWTC	3 cut(s)	208, 338, 384
Tru1I	TTAA	4 cut(s)	17, 893, 941, 966
Tru9I	TTAA	4 cut(s)	17, 893, 941, 966
TscAI	CASTG	2 cut(s)	1105, 1192
TseFI	GTSAC	3 cut(s)	221, 554, 1096
TseI	GCWGC	3 cut(s)	50, 281, 677
Tsp45I	GTSAC	3 cut(s)	221, 554, 1096
TspGWI	ACGGA	1 cut(s)	1028
TspRI	CASTG	2 cut(s)	1105, 1192
VneI	GTGCAC	1 cut(s)	852
VpaK11BI	GGWCC	2 cut(s)	1079, 1110
XapI	RAATTY	2 cut(s)	304, 349
XceI	RCATGY	1 cut(s)	462
XcmI	CCANNNNNNNNNTGG	2 cut(s)	192, 1155
XhoI	CTCGAG	1 cut(s)	1208
XmnI	GAANNNNTTC	1 cut(s)	212
XspI	CTAG	2 cut(s)	173, 485
ZrmI	AGTACT	2 cut(s)	130, 1065

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.