AT5G08390

May participate in a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005757
Physical Location & Seq
Forward (+)
2700959 .. 2707273
6315 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G08390.1

Sequence Viewer

Length: 2520 bp
ATGAACACCAAGCGCGCCTACAAGCTCCAGGAATTTGTGGCACATTCTGCTGCTGTTAATTGTCTTAAGATTGGGAGGAAGTCCTCCAGGGTTCTCGTTACAGGCGGAGAAGATCACAAGGTTAATCTATGGGCTATTGGTAAGCCCAACGCCATCCTGAGCTTGTATGGGCACTCTAGCGGGATTGATTCAGTAACATTTGATGCTTCGGAAGGCTTAGTAGCAGCAGGAGCTGCTAGTGGTACAATTAAACTTTGGGATCTGGAGGAAGCAAAGGTTGTCAGAACTCTCACTGGTCACAGGTCAAACTGCGTATCCGTGAATTTTCACCCTTTTGGTGAGTTTTTTGCTTCTGGTTCTCTAGACACAAATCTCAAGATATGGGATATAAGAAAGAAGGGCTGCATCCATACTTACAAGGGTCACACTAGAGGGGTCAACGTACTCAGATTCACTCCAGATGGTCGTTGGATTGTATCTGGAGGAGAAGATAATGTTGTCAAGGTGTGGGATTTGACTGCTGGAAAACTTTTGCATGAGTTTAAGAGTCATGAAGGGAAAATTCAGAGTCTAGATTTTCATCCCCATGAATTTCTGCTGGCAACAGGTTCAGCTGATAAGACTGTAAAATTCTGGGACCTTGAAACATTCGAGCTTATTGGTTCTGGTGGAACTGAGACTACTGGGGTCCGTTGCCTAACCTTTAATCCAGATGGAAAGAGTGTGCTTTGTGGATTGCAAGAAAGTCTTAAAATTTTCTCATGGGAGCCAATAAGATGTCATGATGGAGTGGATGTTGGATGGTCAAACTTGTCGGATATGAATGTTCACGAGGGAAAGCTTCTCGGTTGCTCATACAATCAAAATTGTGTAGGCGTTTGGGTTGTAGACCTCTCGCGCACTGAGCCTATGAGTGGAGGCGCCACTCAGTCAAATTCTCATCCAGAGAAAACATCAGGCTCAGGCAGAGATCAAGCAGGTCTGAACGATAACAGCTCAAAGGTTATTCTCGGAAAGTTGCCTGGTTCCCAGAAAGTGGATCCTTTATTGAAGGAAACAAAGTCTCTTGGAAAATTATCAGTTTCTCAAAACTCGGATCCGTTGCCAAAAGATACAAAGTCTACTGGAAGGTCATCAGTATCTCAAAGTTCAGATCCTTTGGTAAAGGAACCAAAGCCTCTCGGAAGGTTTTCAGCTACTCACAGCTCAGACACAGTAAAAGAGTCAAGAACCCTGTCATCCACAGGAAGTGTATCAGACTCTCCTCACAGGGTTACTTTGACCAGTGCTCCAAAAAGTGCTTCTGGTATTTCTACAGTTGTCCCTAATGCTGCAGCCTCAAAGAGAAATTTTGGAAAAGCTAACCCAAAGGCGAATCCTCCAGTAGTCAATAAAGAGGATTATTTTCCAGTAATCGTCCCCAGAACGGAGCCAATAATTGAGCAGGCATCTGAATCCAGAGCAGAACTTGACATTATCGGAAGAACTATGCCATATTCGCTGCAGTCAAAGGCCGCTGATTCTCGAAGGCTGTCGAGCAGCAGAAATGAGCCAGATCTACCAACCAGTTCGCTTCTTGAAAGGTCTCAGTCTCAACCTATAGAACCAATCACTTTACAAGATGGGAATACATATCCTAGTGACGAAGGTGGCTCCTGGGATACAGCTGAGAGAACAAATAAAGAGAGCAAATGCAGGGTATTTGGAAGGTTTAATTCAAGATCACTGGTGAGATCACCTCCGAGAAATCACGACGAAAACTCTGACTTGATTAGCTATAATGCAAATAGAGATTCAAGTCCTACTGAAAGCCGAAAAGGAGGAAGACTGCATTCCCTCGTTCTAAATAGGGAAAGAAGAGGAAGATTTTCTAACTTTGAGGGTCCTGTTTCGAGTTCTTCTGGTGGAAATATGACTGCACCAAACAGTCGCCCTTCCAATATGCTCAAGCAGAGAGGAAATCATGTGCCAGTTGATCAAGGAATCACTTCAGCTAGCGAAGAAGATATAGTTGCAGACATAATGGGGCAACACGATCAATTTGTCAGCTCTATGCACTCTCGTTTGGCTAAGTTACAGGTCGTCCGTAGATATTGGGAAAGAAATGATATTAAAAACTCGATAAGTTCAATAGAGAAGATGGCTGATAATGCTGTAATTGCGGATGTACTACTTATAGTAAACGAGAGACCTGAGATTTTGACACTGGATACCTGTACCTCTCTTCTCCCCCTTTTGACAGCTCTTCTAGGGAGCAACATGGATAGTCATTTGAGTGTCTGCCTTGATTTGCTGCTTAAGCTGGTAAGAATGTATGGGTCACAAATTTACTCGTCGCTTTCCGCTCCCTCATCTGTTGGTGTAGATATTGAGGCTGAGCAAAGGATGGAGCGGTACAGTTGTTGCTTTGTCGAGTTCGAGAAAATCAAGGCTTGCCTTCCCTCTTTAGCAAGAAGAGGAAATTTGGTGGCCAAGACTCTCCATGAACTCAACTTAACATTTCAAGAAGTTTCATCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0031461 GO:0032991 GO:0044424 GO:0044464 GO:0080008 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Katanin_con80 WD40

Protein Analysis

839

Amino Acids

91.61

Weight (kDa)

8.38

Isoelectric Point (pI)

44.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR36-Utp21_1st PF25171 2 - 128 1.6e-07 WDR36/Utp21 first beta-propeller
WD40_MABP1-WDR62_2nd PF24782 3 - 172 9.9e-13 MABP1/WDR62 second WD40 domain
Beta-prop_WDR3_1st PF25173 9 - 130 1.3e-22 WDR3 first beta-propeller domain
Beta-prop_TEP1_2nd PF25047 9 - 131 1.8e-10 TEP-1 second beta-propeller
WD40_Gbeta PF25391 10 - 132 1.8e-13 G protein beta WD-40 repeat protein
Beta-prop_WDR5 PF25175 11 - 137 2.4e-25 WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 13 - 137 1.3e-13 WDR36/Utp21 second beta-propeller domain
WD40_Prp19 PF24814 15 - 255 1.1e-47 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 16 - 97 6.2e-10 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 28 - 180 2e-16 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 28 - 128 1.1e-06 WDR90/POC16, second beta-propeller
Beta-prop_SCAP PF24017 28 - 129 4.8e-09 SCAP Beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 34 - 170 3.3e-13 WDR36/Utp21 first beta-propeller
Beta-prop_WDR3_2nd PF25172 52 - 174 3.6e-19 WDR3 second beta-propeller domain
WD40_CDC20-Fz PF24807 52 - 249 6.6e-33 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 53 - 197 4.2e-33 THOC3 beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 54 - 138 9.5e-06 CAF1B/HIR1 beta-propeller domain
WDR55 PF24796 62 - 258 3.5e-22 WDR55
WD40_WDHD1_1st PF24817 73 - 141 8.9e-12 WDHD1 first WD40 domain
WD40 PF00400 92 - 129 7.1e-09 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 96 - 260 1.2e-35 WDR5 beta-propeller domain
Beta-prop_Aladin PF25460 101 - 196 1.3e-08 Aladin seven-bladed propeller
Beta-prop_WDR3_1st PF25173 114 - 250 1e-29 WDR3 first beta-propeller domain
WD40_Gbeta PF25391 120 - 260 9.6e-15 G protein beta WD-40 repeat protein
Beta-prop_SPT8 PF23798 124 - 198 4.1e-07 SPT8 beta propeller
Beta-prop_WDR36-Utp21_2nd PF25168 129 - 202 6.8e-11 WDR36/Utp21 second beta-propeller domain
WD40 PF00400 135 - 171 6.8e-12 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 137 - 218 9e-10 CAF1B/HIR1 beta-propeller domain
EIF3I PF24805 140 - 249 1.3e-15 EIF3I
Beta-prop_THOC3 PF25174 142 - 248 4.9e-22 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 145 - 218 2.7e-11 WDHD1 first WD40 domain
Beta-prop_WDR90_POC16_2nd PF23393 163 - 243 2.6e-08 WDR90/POC16, second beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 167 - 255 5.5e-06 WDR36/Utp21 first beta-propeller
Beta-prop_WDR3_2nd PF25172 169 - 218 2.1e-07 WDR3 second beta-propeller domain
WD40 PF00400 175 - 213 1.7e-09 WD domain, G-beta repeat
Beta-prop_EML_2 PF23414 190 - 296 3.3e-08 Echinoderm microtubule-associated protein second beta-propeller
Katanin_con80 PF13925 677 - 833 1.5e-49 con80 domain of Katanin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 968
AccB1I GGYRCC 1 cut(s) 920
AccB7I CCANNNNNTGG 1 cut(s) 1036
AccBSI CCGCTC 2 cut(s) 2345, 2392
AccI GTMKAC 2 cut(s) 888, 1121
AccII CGCG 2 cut(s) 15, 898
AciI CCGC 6 cut(s) 105, 180, 1515, 2162, 2343, 2392
AclWI GGATC 6 cut(s) 267, 1034, 1047, 1091, 1104, 1148
AcoI YGGCCR 1 cut(s) 2469
AcuI CTGAAG 1 cut(s) 1974
AcyI GRCGYC 1 cut(s) 921
AfaI GTAC 5 cut(s) 244, 444, 2169, 2218, 2396
AfiI CCNNNNNNNGG 3 cut(s) 914, 1036, 1426
AflII CTTAAG 2 cut(s) 65, 2297
AgsI TTSAA 7 cut(s) 644, 1051, 1580, 1719, 1797, 2130, 2504
AjnI CCWGG 4 cut(s) 27, 86, 1021, 1655
AjuI GAANNNNNNNTTGG 2 cut(s) 1285, 1317
Alw21I GWGCWC 1 cut(s) 1291
Alw26I GTCTC 5 cut(s) 671, 1068, 1590, 1596, 2182
AlwI GGATC 6 cut(s) 267, 1034, 1047, 1091, 1104, 1148
AlwNI CAGNNNCTG 1 cut(s) 233
AoxI GGCC 2 cut(s) 1512, 2469
ApeKI GCWGC 9 cut(s) 50, 224, 233, 402, 1331, 1334, 1501, 1539, 2293
Asp700I GAANNNNTTC 3 cut(s) 1189, 1867, 1987
AspLEI GCGC 4 cut(s) 15, 17, 900, 923
AspS9I GGNCC 3 cut(s) 637, 688, 1883
AsuHPI GGTGA 4 cut(s) 320, 350, 1728, 1741
AsuNHI GCTAGC 1 cut(s) 1994
AvaII GGWCC 3 cut(s) 637, 688, 1883
BaeGI GKGCMC 1 cut(s) 174
BaeI ACNNNNGTAYC 2 cut(s) 2386, 2419
BalI TGGCCA 1 cut(s) 2471
BamHI GGATCC 2 cut(s) 1039, 1096
BanI GGYRCC 1 cut(s) 920
BauI CACGAG 1 cut(s) 830
BbsI GAAGAC 1 cut(s) 1831
Bbv12I GWGCWC 1 cut(s) 1291
BbvI GCAGC 9 cut(s) 37, 220, 236, 389, 1318, 1346, 1488, 1551, 2280
BccI CCATC 8 cut(s) 161, 455, 707, 779, 795, 1616, 2134, 2380
BciT130I CCWGG 4 cut(s) 29, 88, 1023, 1657
BciVI GTATCC 3 cut(s) 325, 1654, 2203
BclI TGATCA 1 cut(s) 1975
BcoDI GTCTC 5 cut(s) 671, 1068, 1590, 1596, 2182
BfaI CTAG 8 cut(s) 177, 237, 362, 429, 572, 1638, 1995, 2249
BfmI CTRYAG 4 cut(s) 1314, 1332, 1502, 1599
BfoI RGCGCY 1 cut(s) 924
BfrI CTTAAG 2 cut(s) 65, 2297
BfuAI ACCTGC 1 cut(s) 968
BfuI GTATCC 3 cut(s) 325, 1654, 2203
BglII AGATCT 1 cut(s) 1555
BlpI GCTNAGC 1 cut(s) 2376
Bme1390I CCNGG 4 cut(s) 29, 88, 1023, 1657
Bme18I GGWCC 3 cut(s) 637, 688, 1883
BmgT120I GGNCC 3 cut(s) 637, 688, 1883
BmrFI CCNGG 4 cut(s) 29, 88, 1023, 1657
BmrI ACTGGG 1 cut(s) 693
BmsI GCATC 3 cut(s) 193, 414, 1457
BmtI GCTAGC 1 cut(s) 1998
BmuI ACTGGG 1 cut(s) 693
BpiI GAAGAC 1 cut(s) 1831
BplI GAGNNNNNCTC 2 cut(s) 1723, 1755
BpmI CTGGAG 6 cut(s) 11, 70, 284, 441, 501, 1365
Bpu10I CCTNAGC 2 cut(s) 158, 961
Bpu1102I GCTNAGC 1 cut(s) 2376
BpuEI CTTGAG 2 cut(s) 359, 1931
BsaBI GATNNNNATC 1 cut(s) 579
BsaHI GRCGYC 1 cut(s) 921
BsaI GGTCTC 2 cut(s) 1590, 2182
BsaJI CCNNGG 2 cut(s) 87, 1656
BsaXI ACNNNNNCTCC 4 cut(s) 780, 810, 1273, 1303
Bsc4I CCNNNNNNNGG 3 cut(s) 914, 1036, 1426
Bse8I GATNNNNATC 1 cut(s) 579
BseBI CCWGG 4 cut(s) 29, 88, 1023, 1657
BseDI CCNNGG 2 cut(s) 87, 1656
BseGI GGATG 9 cut(s) 153, 405, 580, 799, 806, 940, 1238, 2170, 2391
BseJI GATNNNNATC 1 cut(s) 579
BseLI CCNNNNNNNGG 3 cut(s) 914, 1036, 1426
BsePI GCGCGC 1 cut(s) 13
BseRI GAGGAG 2 cut(s) 498, 1254
BseSI GKGCMC 1 cut(s) 174
BseXI GCAGC 9 cut(s) 37, 220, 236, 389, 1318, 1346, 1488, 1551, 2280
BsgI GTGCAG 1 cut(s) 1902
Bsh1236I CGCG 2 cut(s) 15, 898
BshFI GGCC 2 cut(s) 1514, 2471
BshNI GGYRCC 1 cut(s) 920
BsiHKAI GWGCWC 1 cut(s) 1291
BslFI GGGAC 3 cut(s) 650, 1307, 1403
BslI CCNNNNNNNGG 3 cut(s) 914, 1036, 1426
BsmAI GTCTC 5 cut(s) 671, 1068, 1590, 1596, 2182
BsmFI GGGAC 3 cut(s) 650, 1307, 1403
BsmI GAATGC 1 cut(s) 1831
BsnI GGCC 2 cut(s) 1514, 2471
Bso31I GGTCTC 2 cut(s) 1590, 2182
Bsp1286I GDGCHC 2 cut(s) 174, 1291
Bsp1720I GCTNAGC 1 cut(s) 2376
BspACI CCGC 6 cut(s) 105, 180, 1515, 2162, 2343, 2392
BspANI GGCC 2 cut(s) 1514, 2471
BspFNI CGCG 2 cut(s) 15, 898
BspHI TCATGA 2 cut(s) 550, 781
BspMAI CTGCAG 2 cut(s) 1336, 1506
BspMI ACCTGC 1 cut(s) 968
BspOI GCTAGC 1 cut(s) 1998
BspPI GGATC 6 cut(s) 267, 1034, 1047, 1091, 1104, 1148
BspQI GCTCTTC 1 cut(s) 2250
BspT107I GGYRCC 1 cut(s) 920
BspTI CTTAAG 2 cut(s) 65, 2297
BspTNI GGTCTC 2 cut(s) 1590, 2182
BsrBI CCGCTC 2 cut(s) 2345, 2392
BssECI CCNNGG 2 cut(s) 87, 1656
BssHII GCGCGC 1 cut(s) 13
BssNI GRCGYC 1 cut(s) 921
BssSI CACGAG 1 cut(s) 830
Bst2BI CACGAG 1 cut(s) 830
Bst2UI CCWGG 4 cut(s) 29, 88, 1023, 1657
Bst4CI ACNGT 5 cut(s) 625, 1216, 1318, 1928, 2399
Bst6I CTCTTC 4 cut(s) 1852, 2229, 2250, 2449
BstACI GRCGYC 1 cut(s) 921
BstAFI CTTAAG 2 cut(s) 65, 2297
BstAPI GCANNNNNTGC 2 cut(s) 47, 233
BstC8I GCNNGC 5 cut(s) 15, 600, 1446, 1996, 2434
BstF5I GGATG 9 cut(s) 153, 405, 580, 799, 806, 940, 1238, 2170, 2391
BstFNI CGCG 2 cut(s) 15, 898
BstH2I RGCGCY 1 cut(s) 924
BstHHI GCGC 4 cut(s) 15, 17, 900, 923
BstMAI GTCTC 5 cut(s) 671, 1068, 1590, 1596, 2182
BstMWI GCNNNNNNNGC 8 cut(s) 47, 230, 233, 904, 1498, 2150, 2159, 2299
BstNI CCWGG 4 cut(s) 29, 88, 1023, 1657
BstSCI CCNGG 4 cut(s) 27, 86, 1021, 1655
BstSFI CTRYAG 4 cut(s) 1314, 1332, 1502, 1599
BstSLI GKGCMC 1 cut(s) 174
BstUI CGCG 2 cut(s) 15, 898
BstV1I GCAGC 9 cut(s) 37, 220, 236, 389, 1318, 1346, 1488, 1551, 2280
BstV2I GAAGAC 1 cut(s) 1831
BstX2I RGATCY 5 cut(s) 259, 1039, 1096, 1153, 1555
BstYI RGATCY 5 cut(s) 259, 1039, 1096, 1153, 1555
BsuI GTATCC 3 cut(s) 325, 1654, 2203
BsuRI GGCC 2 cut(s) 1514, 2471
BtsCI GGATG 9 cut(s) 153, 405, 580, 799, 806, 940, 1238, 2170, 2391
BtsIMutI CAGTG 5 cut(s) 291, 900, 1291, 1724, 2204
BveI ACCTGC 1 cut(s) 968
Cac8I GCNNGC 5 cut(s) 15, 600, 1446, 1996, 2434
CaiI CAGNNNCTG 1 cut(s) 233
CciI TCATGA 2 cut(s) 550, 781
CfoI GCGC 4 cut(s) 15, 17, 900, 923
Cfr13I GGNCC 3 cut(s) 637, 688, 1883
Csp6I GTAC 5 cut(s) 243, 443, 2168, 2217, 2395
CviAII CATG 8 cut(s) 536, 551, 587, 762, 782, 1964, 2260, 2483
CviQI GTAC 5 cut(s) 243, 443, 2168, 2217, 2395
DinI GGCGCC 1 cut(s) 922
EaeI YGGCCR 1 cut(s) 2469
Eam1104I CTCTTC 4 cut(s) 1852, 2229, 2250, 2449
EarI CTCTTC 4 cut(s) 1852, 2229, 2250, 2449
EciI GGCGGA 1 cut(s) 120
Eco31I GGTCTC 2 cut(s) 1590, 2182
Eco47I GGWCC 3 cut(s) 637, 688, 1883
Eco57I CTGAAG 1 cut(s) 1974
EcoO109I RGGNCCY 2 cut(s) 637, 1883
EcoRII CCWGG 4 cut(s) 27, 86, 1021, 1655
EgeI GGCGCC 1 cut(s) 922
EheI GGCGCC 1 cut(s) 922
FaeI CATG 8 cut(s) 539, 554, 590, 765, 785, 1967, 2263, 2486
FalI AAGNNNNNCTT 2 cut(s) 732, 764
FaqI GGGAC 3 cut(s) 650, 1307, 1403
FatI CATG 8 cut(s) 535, 550, 586, 761, 781, 1963, 2259, 2482
FauI CCCGC 1 cut(s) 173
FbaI TGATCA 1 cut(s) 1975
FblI GTMKAC 2 cut(s) 888, 1121
FokI GGATG 9 cut(s) 140, 392, 567, 806, 813, 927, 1225, 2177, 2398
FspBI CTAG 8 cut(s) 177, 237, 362, 429, 572, 1638, 1995, 2249
GlaI GCGC 4 cut(s) 14, 16, 899, 922
GsuI CTGGAG 6 cut(s) 11, 70, 284, 441, 501, 1365
HaeII RGCGCY 1 cut(s) 924
HaeIII GGCC 2 cut(s) 1514, 2471
HhaI GCGC 4 cut(s) 15, 17, 900, 923
Hin1I GRCGYC 1 cut(s) 921
Hin1II CATG 8 cut(s) 539, 554, 590, 765, 785, 1967, 2263, 2486
Hin6I GCGC 4 cut(s) 13, 15, 898, 921
HinP1I GCGC 4 cut(s) 13, 15, 898, 921
HincII GTYRAC 1 cut(s) 439
HindII GTYRAC 1 cut(s) 439
HindIII AAGCTT 1 cut(s) 839
HphI GGTGA 4 cut(s) 320, 350, 1728, 1741
Hpy166II GTNNAC 5 cut(s) 439, 829, 889, 1122, 2182
Hpy8I GTNNAC 5 cut(s) 439, 829, 889, 1122, 2182
Hpy99I CGWCG 2 cut(s) 1757, 2338
HpyCH4III ACNGT 5 cut(s) 625, 1216, 1318, 1928, 2399
HpyCH4IV ACGT 1 cut(s) 441
HpyF10VI GCNNNNNNNGC 8 cut(s) 47, 230, 233, 904, 1498, 2150, 2159, 2299
HpySE526I ACGT 1 cut(s) 441
Hsp92I GRCGYC 1 cut(s) 921
Hsp92II CATG 8 cut(s) 539, 554, 590, 765, 785, 1967, 2263, 2486
HspAI GCGC 4 cut(s) 13, 15, 898, 921
KasI GGCGCC 1 cut(s) 920
Ksp22I TGATCA 1 cut(s) 1975
LguI GCTCTTC 1 cut(s) 2250
LmnI GCTCC 9 cut(s) 30, 230, 766, 1294, 1429, 1658, 2253, 2350, 2389
Lsp1109I GCAGC 9 cut(s) 37, 220, 236, 389, 1318, 1346, 1488, 1551, 2280
LweI GCATC 3 cut(s) 193, 414, 1457
MaeI CTAG 8 cut(s) 177, 237, 362, 429, 572, 1638, 1995, 2249
MaeII ACGT 1 cut(s) 441
MaeIII GTNAC 8 cut(s) 97, 193, 296, 422, 1273, 1640, 2073, 2319
MbiI CCGCTC 2 cut(s) 2345, 2392
MflI RGATCY 5 cut(s) 259, 1039, 1096, 1153, 1555
MhlI GDGCHC 2 cut(s) 174, 1291
MlsI TGGCCA 1 cut(s) 2471
MluNI TGGCCA 1 cut(s) 2471
Mly113I GGCGCC 1 cut(s) 921
MlyI GAGTC 5 cut(s) 556, 577, 1232, 1253, 2470
MmeI TCCRAC 3 cut(s) 449, 778, 795
Mox20I TGGCCA 1 cut(s) 2471
MroXI GAANNNNTTC 3 cut(s) 1189, 1867, 1987
MscI TGGCCA 1 cut(s) 2471
MslI CAYNNNNRTG 2 cut(s) 585, 2274
Msp20I TGGCCA 1 cut(s) 2471
MspA1I CMGCKG 3 cut(s) 614, 1517, 1667
MspCI CTTAAG 2 cut(s) 65, 2297
MspR9I CCNGG 4 cut(s) 29, 88, 1023, 1657
Mva1269I GAATGC 1 cut(s) 1831
MvaI CCWGG 4 cut(s) 29, 88, 1023, 1657
MvnI CGCG 2 cut(s) 15, 898
MwoI GCNNNNNNNGC 8 cut(s) 47, 230, 233, 904, 1498, 2150, 2159, 2299
NarI GGCGCC 1 cut(s) 921
NheI GCTAGC 1 cut(s) 1994
NlaIII CATG 8 cut(s) 539, 554, 590, 765, 785, 1967, 2263, 2486
NmuCI GTSAC 4 cut(s) 296, 422, 1640, 2319
PagI TCATGA 2 cut(s) 550, 781
PauI GCGCGC 1 cut(s) 13
PciSI GCTCTTC 1 cut(s) 2250
PctI GAATGC 1 cut(s) 1831
PdmI GAANNNNTTC 3 cut(s) 1189, 1867, 1987
PfeI GAWTC 7 cut(s) 188, 450, 1375, 1454, 1520, 1793, 1983
PflMI CCANNNNNTGG 1 cut(s) 1036
PfoI TCCNGGA 1 cut(s) 27
PleI GAGTC 5 cut(s) 555, 576, 1231, 1253, 2470
PluTI GGCGCC 1 cut(s) 924
PpsI GAGTC 5 cut(s) 555, 576, 1231, 1253, 2470
PpuMI RGGWCCY 2 cut(s) 637, 1883
Psp5II RGGWCCY 2 cut(s) 637, 1883
Psp6I CCWGG 4 cut(s) 27, 86, 1021, 1655
PspGI CCWGG 4 cut(s) 27, 86, 1021, 1655
PspPI GGNCC 3 cut(s) 637, 688, 1883
PspPPI RGGWCCY 2 cut(s) 637, 1883
PsrI GAACNNNNNNTAC 2 cut(s) 664, 696
PstI CTGCAG 2 cut(s) 1336, 1506
PstNI CAGNNNCTG 1 cut(s) 233
PsuI RGATCY 5 cut(s) 259, 1039, 1096, 1153, 1555
PteI GCGCGC 1 cut(s) 13
PvuII CAGCTG 2 cut(s) 614, 1667
RsaI GTAC 5 cut(s) 244, 444, 2169, 2218, 2396
RsaNI GTAC 5 cut(s) 243, 443, 2168, 2217, 2395
RseI CAYNNNNRTG 2 cut(s) 585, 2274
SapI GCTCTTC 1 cut(s) 2250
Sau96I GGNCC 3 cut(s) 637, 688, 1883
SchI GAGTC 5 cut(s) 556, 577, 1232, 1253, 2470
ScrFI CCNGG 4 cut(s) 29, 88, 1023, 1657
SduI GDGCHC 2 cut(s) 174, 1291
SfaNI GCATC 3 cut(s) 193, 414, 1457
SfcI CTRYAG 4 cut(s) 1314, 1332, 1502, 1599
SfoI GGCGCC 1 cut(s) 922
SinI GGWCC 3 cut(s) 637, 688, 1883
SmiMI CAYNNNNRTG 2 cut(s) 585, 2274
SmlI CTYRAG 4 cut(s) 65, 374, 1946, 2297
SmoI CTYRAG 4 cut(s) 65, 374, 1946, 2297
SsiI CCGC 6 cut(s) 105, 180, 1515, 2162, 2343, 2392
SspDI GGCGCC 1 cut(s) 920
SspMI CTAG 8 cut(s) 177, 237, 362, 429, 572, 1638, 1995, 2249
StyD4I CCNGG 4 cut(s) 27, 86, 1021, 1655
TaaI ACNGT 5 cut(s) 625, 1216, 1318, 1928, 2399
TaiI ACGT 1 cut(s) 444
TaqI TCGA 7 cut(s) 651, 1525, 1535, 1892, 2120, 2412, 2418
TatI WGTACW 1 cut(s) 2167
TauI GCSGC 1 cut(s) 1517
TfiI GAWTC 7 cut(s) 188, 450, 1375, 1454, 1520, 1793, 1983
TscAI CASTG 5 cut(s) 298, 907, 1291, 1731, 2211
TseFI GTSAC 4 cut(s) 296, 422, 1640, 2319
TseI GCWGC 9 cut(s) 50, 224, 233, 402, 1331, 1334, 1501, 1539, 2293
Tsp45I GTSAC 4 cut(s) 296, 422, 1640, 2319
TspDTI ATGAA 7 cut(s) 17, 567, 569, 603, 836, 2499, 2502
TspGWI ACGGA 5 cut(s) 307, 680, 1089, 1442, 2075
TspRI CASTG 5 cut(s) 298, 907, 1291, 1731, 2211
Van91I CCANNNNNTGG 1 cut(s) 1036
Vha464I CTTAAG 2 cut(s) 65, 2297
VpaK11BI GGWCC 3 cut(s) 637, 688, 1883
XbaI TCTAGA 2 cut(s) 361, 571
XcmI CCANNNNNNNNNTGG 1 cut(s) 465
XmiI GTMKAC 2 cut(s) 888, 1121
XmnI GAANNNNTTC 3 cut(s) 1189, 1867, 1987
XspI CTAG 8 cut(s) 177, 237, 362, 429, 572, 1638, 1995, 2249
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.