Rorug04G0379100

May participate in a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0005757
Physical Location & Seq
Reverse (-)
54977981 .. 54981546
3566 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0379100.1

Sequence Viewer

Length: 1044 bp
ATGCTAGCCAGAACGACGCCGTCTCCACTGCTCACTTTTGCCACTACAAAACTGGTGTGCTGCTGCTTCCACAGTTTGGCACCAAACAAGATGGTGAAGCTGATCGTGGCGACGACGACCGATCCGGCCTCCGTCAACCCTGCCAATGCTCTCTTATCCAAGCCCGGCTGGTCCCCAACCCCTTCTCTTCTACAGGAGGATATGAAGAGCTTCGCTAACGGAGCCGTTAGGGTTTTGCTGCACAACAGGAGCATCATCGAAGAGGACGACTTGGACGCTCGCTGGGAACAAGCCACCGGCGAACACGTGGACGAGCTCATCTTCTTGAGCAAGCACAAAGCCGCTTCCAGCCGCCCTGCGCTTACTGTTCACCCCATCGGGGTTCCTCATTTGCGAGACGGCGACGTTTTGCCGCAGGGCGGGAAGCCGGGCTGGGCCGCGCCGCCGAATCCGAGGATTGGGCCCTGGCTCAGGCTCTTGAAGAGAATCGCGCAAGATCACAACTTGGTCCCTGAGTTTGAGGTGACTTTGGAGGCTACACATCATGGACCGATTACAGACACGCCTGCCATGTTCCTGGAGATTGGCAGTACAGATGATTGCTGGAAGAGGCAGGATGCAGCTGAAGTTATGGCTCTATTAGTTTGGGAAGGACTTGGGCTTGGAGGGGGAGCTGCTGTTGGAAACTGGAACGGGGGGAACGATAATAAAAGAGTTCTGCTTGGGATAGGTGGTGGACATTATGTACCTCGTCATATGGATATAGCTCTGAAAAATGATGTCTGGGTGGGCCATCTAATTTCGGGTTATTCGTTACCAATGGAAGATCCGAGCCAGTCCAAGACAGAAACAAATACAAAAGATATCGGTGGAACTTGGAAACAATCAATCAAAGTAGCATTTGAGGCTACGCAATCGGCTTTCCCTGGTGGAGAAATTGTAGCTCATCTTGATCACAAGAGTTTCAAAAGCTGGCAAAAGAATGCTATAACGAGTTTCCTAGAGGAGCAGAACATTACAATTAGCAAACGCGATTTCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0031461 GO:0032991 GO:0044424 GO:0044464 GO:0080008 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Katanin_con80 WD40

Protein Analysis

347

Amino Acids

37.87

Weight (kDa)

6.27

Isoelectric Point (pI)

49.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_deacylase PF04414 100 - 341 3.6e-58 D-aminoacyl-tRNA deacylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 79
AccB7I CCANNNNNTGG 1 cut(s) 76
AccII CGCG 3 cut(s) 440, 491, 1032
AciI CCGC 6 cut(s) 342, 352, 413, 420, 438, 443
AclWI GGATC 2 cut(s) 116, 821
AcuI CTGAAG 1 cut(s) 645
AcvI CACGTG 1 cut(s) 307
AcyI GRCGYC 1 cut(s) 17
AfaI GTAC 2 cut(s) 592, 747
AfiI CCNNNNNNNGG 5 cut(s) 76, 379, 419, 458, 471
AflIII ACRYGT 1 cut(s) 304
AgsI TTSAA 2 cut(s) 481, 967
AjnI CCWGG 3 cut(s) 464, 576, 925
AluBI AGCT 8 cut(s) 100, 210, 316, 623, 674, 767, 944, 972
AluI AGCT 8 cut(s) 100, 210, 316, 623, 674, 767, 944, 972
Alw21I GWGCWC 1 cut(s) 318
Alw26I GTCTC 2 cut(s) 27, 390
AlwI GGATC 2 cut(s) 116, 821
AoxI GGCC 4 cut(s) 126, 435, 461, 790
ApaI GGGCCC 1 cut(s) 465
ApeKI GCWGC 5 cut(s) 60, 63, 238, 620, 674
Asp700I GAANNNNTTC 1 cut(s) 209
AspLEI GCGC 3 cut(s) 361, 442, 493
AspS9I GGNCC 7 cut(s) 171, 435, 461, 462, 508, 548, 790
AsuC2I CCSGG 2 cut(s) 165, 429
AsuHPI GGTGA 3 cut(s) 106, 362, 535
AsuNHI GCTAGC 1 cut(s) 4
AvaII GGWCC 3 cut(s) 171, 508, 548
BaeGI GKGCMC 1 cut(s) 465
BanI GGYRCC 1 cut(s) 79
BanII GRGCYC 2 cut(s) 318, 465
BbrPI CACGTG 1 cut(s) 307
Bbv12I GWGCWC 1 cut(s) 318
BbvI GCAGC 5 cut(s) 47, 50, 225, 632, 661
BccI CCATC 3 cut(s) 85, 383, 801
BceAI ACGGC 3 cut(s) 4, 209, 415
BciT130I CCWGG 3 cut(s) 466, 578, 927
BclI TGATCA 1 cut(s) 952
BcnI CCSGG 2 cut(s) 165, 429
BcoDI GTCTC 2 cut(s) 27, 390
BfaI CTAG 2 cut(s) 5, 1001
BfmI CTRYAG 1 cut(s) 191
Bme1390I CCNGG 5 cut(s) 165, 429, 466, 578, 927
Bme18I GGWCC 3 cut(s) 171, 508, 548
BmgT120I GGNCC 7 cut(s) 171, 435, 461, 462, 508, 548, 790
BmiI GGNNCC 6 cut(s) 81, 173, 223, 384, 463, 510
BmrFI CCNGG 5 cut(s) 165, 429, 466, 578, 927
BmsI GCATC 2 cut(s) 261, 607
BmtI GCTAGC 1 cut(s) 8
BpmI CTGGAG 1 cut(s) 599
Bpu10I CCTNAGC 1 cut(s) 470
BpuEI CTTGAG 1 cut(s) 346
BpuMI CCSGG 2 cut(s) 165, 429
BsaAI YACGTR 1 cut(s) 307
BsaHI GRCGYC 1 cut(s) 17
BsaJI CCNNGG 3 cut(s) 452, 464, 925
BsaXI ACNNNNNCTCC 6 cut(s) 7, 37, 663, 693, 924, 954
Bsc4I CCNNNNNNNGG 5 cut(s) 76, 379, 419, 458, 471
Bse118I RCCGGY 1 cut(s) 296
Bse1I ACTGG 3 cut(s) 57, 692, 835
BseBI CCWGG 3 cut(s) 466, 578, 927
BseDI CCNNGG 3 cut(s) 452, 464, 925
BseGI GGATG 1 cut(s) 622
BseLI CCNNNNNNNGG 5 cut(s) 76, 379, 419, 458, 471
BseMII CTCAG 2 cut(s) 484, 504
BseNI ACTGG 3 cut(s) 57, 692, 835
BseRI GAGGAG 1 cut(s) 1019
BseSI GKGCMC 1 cut(s) 465
BseXI GCAGC 5 cut(s) 47, 50, 225, 632, 661
BseYI CCCAGC 2 cut(s) 282, 432
BsgI GTGCAG 1 cut(s) 224
Bsh1236I CGCG 3 cut(s) 440, 491, 1032
Bsh1285I CGRYCG 1 cut(s) 120
BshFI GGCC 4 cut(s) 128, 437, 463, 792
BshNI GGYRCC 1 cut(s) 79
BsiEI CGRYCG 1 cut(s) 120
BsiHKAI GWGCWC 1 cut(s) 318
BsiSI CCGG 4 cut(s) 125, 165, 297, 428
BslFI GGGAC 2 cut(s) 157, 494
BslI CCNNNNNNNGG 5 cut(s) 76, 379, 419, 458, 471
BsmAI GTCTC 2 cut(s) 27, 390
BsmBI CGTCTC 2 cut(s) 27, 390
BsmFI GGGAC 2 cut(s) 157, 494
BsmI GAATGC 1 cut(s) 988
BsnI GGCC 4 cut(s) 128, 437, 463, 792
Bsp120I GGGCCC 1 cut(s) 461
Bsp1286I GDGCHC 2 cut(s) 318, 465
Bsp143I GATC 5 cut(s) 102, 121, 496, 826, 952
BspACI CCGC 6 cut(s) 342, 352, 413, 420, 438, 443
BspANI GGCC 4 cut(s) 128, 437, 463, 792
BspCNI CTCAG 2 cut(s) 483, 505
BspFNI CGCG 3 cut(s) 440, 491, 1032
BspLI GGNNCC 6 cut(s) 81, 173, 223, 384, 463, 510
BspOI GCTAGC 1 cut(s) 8
BspPI GGATC 2 cut(s) 116, 821
BspQI GCTCTTC 1 cut(s) 200
BspT107I GGYRCC 1 cut(s) 79
BsrFI RCCGGY 1 cut(s) 296
BsrI ACTGG 3 cut(s) 57, 692, 835
BssAI RCCGGY 1 cut(s) 296
BssECI CCNNGG 3 cut(s) 452, 464, 925
BssMI GATC 5 cut(s) 102, 121, 496, 826, 952
BssNI GRCGYC 1 cut(s) 17
Bst2UI CCWGG 3 cut(s) 466, 578, 927
Bst4CI ACNGT 2 cut(s) 74, 367
Bst6I CTCTTC 5 cut(s) 192, 200, 255, 476, 602
BstACI GRCGYC 1 cut(s) 17
BstBAI YACGTR 1 cut(s) 307
BstC8I GCNNGC 5 cut(s) 6, 280, 332, 567, 974
BstDEI CTNAG 2 cut(s) 470, 513
BstENI CCTNNNNNAGG 1 cut(s) 469
BstF5I GGATG 1 cut(s) 622
BstFNI CGCG 3 cut(s) 440, 491, 1032
BstHHI GCGC 3 cut(s) 361, 442, 493
BstKTI GATC 5 cut(s) 105, 124, 499, 829, 955
BstMAI GTCTC 2 cut(s) 27, 390
BstMBI GATC 5 cut(s) 102, 121, 496, 826, 952
BstMCI CGRYCG 1 cut(s) 120
BstMWI GCNNNNNNNGC 2 cut(s) 221, 905
BstNI CCWGG 3 cut(s) 466, 578, 927
BstSCI CCNGG 5 cut(s) 163, 427, 464, 576, 925
BstSFI CTRYAG 1 cut(s) 191
BstSLI GKGCMC 1 cut(s) 465
BstUI CGCG 3 cut(s) 440, 491, 1032
BstV1I GCAGC 5 cut(s) 47, 50, 225, 632, 661
BstX2I RGATCY 1 cut(s) 826
BstXI CCANNNNNNTGG 1 cut(s) 577
BstYI RGATCY 1 cut(s) 826
BsuRI GGCC 4 cut(s) 128, 437, 463, 792
BtsCI GGATG 1 cut(s) 622
BtsI GCAGTG 1 cut(s) 26
BtsIMutI CAGTG 1 cut(s) 26
Cac8I GCNNGC 5 cut(s) 6, 280, 332, 567, 974
CfoI GCGC 3 cut(s) 361, 442, 493
Cfr10I RCCGGY 1 cut(s) 296
Cfr13I GGNCC 7 cut(s) 171, 435, 461, 462, 508, 548, 790
CseI GACGC 2 cut(s) 25, 284
Csp6I GTAC 2 cut(s) 591, 746
CviAII CATG 2 cut(s) 545, 571
CviQI GTAC 2 cut(s) 591, 746
DdeI CTNAG 2 cut(s) 470, 513
DpnI GATC 5 cut(s) 104, 123, 498, 828, 954
DpnII GATC 5 cut(s) 102, 121, 496, 826, 952
Eam1104I CTCTTC 5 cut(s) 192, 200, 255, 476, 602
EarI CTCTTC 5 cut(s) 192, 200, 255, 476, 602
Ecl136II GAGCTC 1 cut(s) 316
Eco24I GRGCYC 2 cut(s) 318, 465
Eco32I GATATC 1 cut(s) 865
Eco47I GGWCC 3 cut(s) 171, 508, 548
Eco53kI GAGCTC 1 cut(s) 316
Eco57I CTGAAG 1 cut(s) 645
Eco72I CACGTG 1 cut(s) 307
EcoICRI GAGCTC 1 cut(s) 316
EcoNI CCTNNNNNAGG 1 cut(s) 469
EcoO109I RGGNCCY 1 cut(s) 462
EcoRII CCWGG 3 cut(s) 464, 576, 925
EcoRV GATATC 1 cut(s) 865
EcoT38I GRGCYC 2 cut(s) 318, 465
Esp3I CGTCTC 2 cut(s) 27, 390
FaeI CATG 2 cut(s) 548, 574
FaiI YATR 9 cut(s) 203, 546, 572, 632, 744, 756, 758, 764, 989
FaqI GGGAC 2 cut(s) 157, 494
FatI CATG 2 cut(s) 544, 570
FauI CCCGC 1 cut(s) 413
FauNDI CATATG 1 cut(s) 756
FbaI TGATCA 1 cut(s) 952
FokI GGATG 1 cut(s) 629
FriOI GRGCYC 2 cut(s) 318, 465
FspBI CTAG 2 cut(s) 5, 1001
GlaI GCGC 3 cut(s) 360, 441, 492
GsaI CCCAGC 2 cut(s) 286, 436
GsuI CTGGAG 1 cut(s) 599
HaeIII GGCC 4 cut(s) 128, 437, 463, 792
HapII CCGG 4 cut(s) 125, 165, 297, 428
HgaI GACGC 2 cut(s) 25, 284
HhaI GCGC 3 cut(s) 361, 442, 493
Hin1I GRCGYC 1 cut(s) 17
Hin1II CATG 2 cut(s) 548, 574
Hin6I GCGC 3 cut(s) 359, 440, 491
HinP1I GCGC 3 cut(s) 359, 440, 491
HincII GTYRAC 1 cut(s) 136
HindII GTYRAC 1 cut(s) 136
HinfI GANTC 2 cut(s) 448, 486
HpaII CCGG 4 cut(s) 125, 165, 297, 428
HphI GGTGA 3 cut(s) 106, 362, 535
Hpy166II GTNNAC 4 cut(s) 136, 310, 370, 737
Hpy188I TCNGA 4 cut(s) 453, 771, 831, 1043
Hpy188III TCNNGA 3 cut(s) 325, 478, 950
Hpy8I GTNNAC 4 cut(s) 136, 310, 370, 737
Hpy99I CGWCG 4 cut(s) 19, 115, 118, 407
HpyAV CCTTC 2 cut(s) 192, 644
HpyCH4III ACNGT 2 cut(s) 74, 367
HpyCH4IV ACGT 2 cut(s) 306, 405
HpyCH4V TGCA 2 cut(s) 241, 620
HpyF10VI GCNNNNNNNGC 2 cut(s) 221, 905
HpyF3I CTNAG 2 cut(s) 470, 513
HpySE526I ACGT 2 cut(s) 306, 405
Hsp92I GRCGYC 1 cut(s) 17
Hsp92II CATG 2 cut(s) 548, 574
HspAI GCGC 3 cut(s) 359, 440, 491
Ksp22I TGATCA 1 cut(s) 952
Kzo9I GATC 5 cut(s) 102, 121, 496, 826, 952
LguI GCTCTTC 1 cut(s) 200
LmnI GCTCC 4 cut(s) 221, 249, 671, 1006
Lsp1109I GCAGC 5 cut(s) 47, 50, 225, 632, 661
LweI GCATC 2 cut(s) 261, 607
MaeI CTAG 2 cut(s) 5, 1001
MaeII ACGT 2 cut(s) 306, 405
MaeIII GTNAC 2 cut(s) 523, 813
MalI GATC 5 cut(s) 104, 123, 498, 828, 954
MboI GATC 5 cut(s) 102, 121, 496, 826, 952
MboII GAAGA 8 cut(s) 179, 217, 272, 313, 493, 619, 836, 1030
MflI RGATCY 1 cut(s) 826
MhlI GDGCHC 2 cut(s) 318, 465
MluCI AATT 3 cut(s) 798, 936, 1020
MmeI TCCRAC 1 cut(s) 661
MroXI GAANNNNTTC 1 cut(s) 209
MspA1I CMGCKG 1 cut(s) 623
MspI CCGG 4 cut(s) 125, 165, 297, 428
MspR9I CCNGG 5 cut(s) 165, 429, 466, 578, 927
Mva1269I GAATGC 1 cut(s) 988
MvaI CCWGG 3 cut(s) 466, 578, 927
MvnI CGCG 3 cut(s) 440, 491, 1032
MwoI GCNNNNNNNGC 2 cut(s) 221, 905
NciI CCSGG 2 cut(s) 165, 429
NdeI CATATG 1 cut(s) 756
NdeII GATC 5 cut(s) 102, 121, 496, 826, 952
NheI GCTAGC 1 cut(s) 4
NlaIII CATG 2 cut(s) 548, 574
NlaIV GGNNCC 6 cut(s) 81, 173, 223, 384, 463, 510
NmuCI GTSAC 1 cut(s) 523
PciSI GCTCTTC 1 cut(s) 200
PcsI WCGNNNNNNNCGW 3 cut(s) 264, 699, 809
PctI GAATGC 1 cut(s) 988
PdmI GAANNNNTTC 1 cut(s) 209
PfeI GAWTC 2 cut(s) 448, 486
PflFI GACNNNGTC 1 cut(s) 19
PflMI CCANNNNNTGG 1 cut(s) 76
PfoI TCCNGGA 1 cut(s) 576
PmaCI CACGTG 1 cut(s) 307
PmlI CACGTG 1 cut(s) 307
Ppu21I YACGTR 1 cut(s) 307
Psp124BI GAGCTC 1 cut(s) 318
Psp6I CCWGG 3 cut(s) 464, 576, 925
PspCI CACGTG 1 cut(s) 307
PspFI CCCAGC 2 cut(s) 282, 432
PspGI CCWGG 3 cut(s) 464, 576, 925
PspN4I GGNNCC 6 cut(s) 81, 173, 223, 384, 463, 510
PspOMI GGGCCC 1 cut(s) 461
PspPI GGNCC 7 cut(s) 171, 435, 461, 462, 508, 548, 790
PsuI RGATCY 1 cut(s) 826
PsyI GACNNNGTC 1 cut(s) 19
PvuII CAGCTG 1 cut(s) 623
RsaI GTAC 2 cut(s) 592, 747
RsaNI GTAC 2 cut(s) 591, 746
SacI GAGCTC 1 cut(s) 318
SapI GCTCTTC 1 cut(s) 200
Sau3AI GATC 5 cut(s) 102, 121, 496, 826, 952
Sau96I GGNCC 7 cut(s) 171, 435, 461, 462, 508, 548, 790
ScrFI CCNGG 5 cut(s) 165, 429, 466, 578, 927
SduI GDGCHC 2 cut(s) 318, 465
SfaNI GCATC 2 cut(s) 261, 607
SfcI CTRYAG 1 cut(s) 191
SgrAI CRCCGGYG 1 cut(s) 296
SinI GGWCC 3 cut(s) 171, 508, 548
SmlI CTYRAG 1 cut(s) 325
SmoI CTYRAG 1 cut(s) 325
Sse9I AATT 3 cut(s) 798, 936, 1020
SsiI CCGC 6 cut(s) 342, 352, 413, 420, 438, 443
SspMI CTAG 2 cut(s) 5, 1001
SstI GAGCTC 1 cut(s) 318
StyD4I CCNGG 5 cut(s) 163, 427, 464, 576, 925
TaaI ACNGT 2 cut(s) 74, 367
TaiI ACGT 2 cut(s) 309, 408
TaqI TCGA 1 cut(s) 258
TaqII GACCGA 2 cut(s) 134, 565
TasI AATT 3 cut(s) 798, 936, 1020
TatI WGTACW 1 cut(s) 590
TauI GCSGC 5 cut(s) 344, 354, 415, 440, 445
TfiI GAWTC 2 cut(s) 448, 486
TscAI CASTG 1 cut(s) 33
TseFI GTSAC 1 cut(s) 523
TseI GCWGC 5 cut(s) 60, 63, 238, 620, 674
Tsp45I GTSAC 1 cut(s) 523
TspDTI ATGAA 1 cut(s) 218
TspGWI ACGGA 2 cut(s) 121, 234
TspRI CASTG 1 cut(s) 33
Tth111I GACNNNGTC 1 cut(s) 19
Van91I CCANNNNNTGG 1 cut(s) 76
VpaK11BI GGWCC 3 cut(s) 171, 508, 548
XagI CCTNNNNNAGG 1 cut(s) 469
XcmI CCANNNNNNNNNTGG 1 cut(s) 49
XmnI GAANNNNTTC 1 cut(s) 209
XspI CTAG 2 cut(s) 5, 1001
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.