Rorug04G0379400

May participate in a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0005757
Physical Location & Seq
Forward (+)
54995256 .. 54996399
1144 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0379400.1

Sequence Viewer

Length: 696 bp
ATGTCACTACCTCAAATCCCTTCTTCTCTTCCCCAACACTCGCAACAATTTGAGCTCAGCCAAGAAACCAGAGACCTAATATCCACGCTTCCTGCTGAGAAAGGATTGATTTCAGGTCATTTACATCAGTACCAGGGCTTTTGGTACCCCACTATGCATATCCAAAAAGTTTTAGCCTCCCAAAAACACTTTCAAGCTCAAGATACTGATATCCTTCTCGCTACTACTCCCAAATCAGGCACCACTTGGCTCAAGGCAATTCTGTTTGCGCTAGTAAAGCGAGTGCACTATCATCCAGACCCACAAAACATGGACCACCCTTTGCTCACAAACAACCCTCATGTTCTTGTGCCCTTCTTGGAGAATAAGCATTTCGGTAAGAACCAGATTCCTGACCCCACCTCTTATGCTCCTCCCAGGCTCTTTTCAACTCACTTACCACTAGCGCATCTGCCACAATCTGTTAAAGACTCTGCTTGCAAGGTTGTCTATCTGTGTAGAAACCCTAAGGACATAATAGTTTCACTTTGGCACTTCACAAACAGAGTGAGACACAAGAGTATGGGAACCAATTCACTTGAAGAAGTTTTCGAATACTTCTGCAGGGGAGTGAGTGGCTGTGTTGCTGCTACTGCTTTTGAAGGAATGGTTGTTGTGCATGAAGCAGGTGATGCATCTCCTCTTGTTGTATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0031461 GO:0032991 GO:0044424 GO:0044464 GO:0080008 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Katanin_con80 WD40

Protein Analysis

231

Amino Acids

26.01

Weight (kDa)

7.81

Isoelectric Point (pI)

43.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sulfotransfer_1 PF00685 68 - 205 1.6e-36 Sulfotransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 656
Acc36I ACCTGC 1 cut(s) 656
Acc65I GGTACC 1 cut(s) 144
AccB1I GGYRCC 2 cut(s) 144, 239
AfaI GTAC 2 cut(s) 131, 146
AfiI CCNNNNNNNGG 1 cut(s) 236
AgsI TTSAA 4 cut(s) 194, 429, 581, 641
AjnI CCWGG 2 cut(s) 132, 416
AjuI GAANNNNNNNTTGG 2 cut(s) 174, 206
AluBI AGCT 2 cut(s) 55, 197
AluI AGCT 2 cut(s) 55, 197
Alw21I GWGCWC 2 cut(s) 57, 288
Alw26I GTCTC 2 cut(s) 66, 544
Alw44I GTGCAC 1 cut(s) 284
ApaLI GTGCAC 1 cut(s) 284
ApeKI GCWGC 1 cut(s) 626
Asp700I GAANNNNTTC 1 cut(s) 571
Asp718I GGTACC 1 cut(s) 144
AspLEI GCGC 2 cut(s) 271, 448
AspS9I GGNCC 1 cut(s) 313
AsuHPI GGTGA 1 cut(s) 680
AsuII TTCGAA 1 cut(s) 591
AvaII GGWCC 1 cut(s) 313
AxyI CCTNAGG 1 cut(s) 507
BaeGI GKGCMC 2 cut(s) 288, 354
BaeI ACNNNNGTAYC 2 cut(s) 113, 146
BanI GGYRCC 2 cut(s) 144, 239
BanII GRGCYC 1 cut(s) 57
Bbv12I GWGCWC 2 cut(s) 57, 288
BbvI GCAGC 1 cut(s) 613
BciT130I CCWGG 2 cut(s) 134, 418
BcoDI GTCTC 2 cut(s) 66, 544
BfaI CTAG 3 cut(s) 272, 443, 694
BfmI CTRYAG 1 cut(s) 601
BfuAI ACCTGC 1 cut(s) 656
BisI GCNGC 1 cut(s) 627
BlpI GCTNAGC 1 cut(s) 56
BlsI GCNGC 1 cut(s) 628
Bme1390I CCNGG 2 cut(s) 134, 418
Bme18I GGWCC 1 cut(s) 313
BmgT120I GGNCC 1 cut(s) 313
BmiI GGNNCC 3 cut(s) 146, 241, 568
BmrFI CCNGG 2 cut(s) 134, 418
BmsI GCATC 3 cut(s) 457, 661, 683
Bpu1102I GCTNAGC 1 cut(s) 56
Bpu14I TTCGAA 1 cut(s) 591
BpuEI CTTGAG 2 cut(s) 183, 236
BsaI GGTCTC 1 cut(s) 66
BsaJI CCNNGG 2 cut(s) 133, 416
Bsc4I CCNNNNNNNGG 1 cut(s) 236
Bse21I CCTNAGG 1 cut(s) 507
BseBI CCWGG 2 cut(s) 134, 418
BseDI CCNNGG 2 cut(s) 133, 416
BseGI GGATG 1 cut(s) 292
BseLI CCNNNNNNNGG 1 cut(s) 236
BseMII CTCAG 2 cut(s) 70, 87
BseRI GAGGAG 2 cut(s) 402, 669
BseSI GKGCMC 2 cut(s) 288, 354
BseXI GCAGC 1 cut(s) 613
BshNI GGYRCC 2 cut(s) 144, 239
BsiHKAI GWGCWC 2 cut(s) 57, 288
BslI CCNNNNNNNGG 1 cut(s) 236
BsmAI GTCTC 2 cut(s) 66, 544
Bso31I GGTCTC 1 cut(s) 66
Bsp119I TTCGAA 1 cut(s) 591
Bsp1286I GDGCHC 3 cut(s) 57, 288, 354
Bsp1720I GCTNAGC 1 cut(s) 56
BspCNI CTCAG 2 cut(s) 69, 88
BspLI GGNNCC 3 cut(s) 146, 241, 568
BspMAI CTGCAG 1 cut(s) 605
BspMI ACCTGC 1 cut(s) 656
BspT104I TTCGAA 1 cut(s) 591
BspT107I GGYRCC 2 cut(s) 144, 239
BspTNI GGTCTC 1 cut(s) 66
BssECI CCNNGG 2 cut(s) 133, 416
Bst2UI CCWGG 2 cut(s) 134, 418
Bst6I CTCTTC 1 cut(s) 33
BstAPI GCANNNNNTGC 1 cut(s) 671
BstBI TTCGAA 1 cut(s) 591
BstC8I GCNNGC 1 cut(s) 478
BstDEI CTNAG 3 cut(s) 56, 96, 507
BstF5I GGATG 1 cut(s) 292
BstHHI GCGC 2 cut(s) 271, 448
BstMAI GTCTC 2 cut(s) 66, 544
BstMWI GCNNNNNNNGC 3 cut(s) 277, 632, 671
BstNI CCWGG 2 cut(s) 134, 418
BstSCI CCNGG 2 cut(s) 132, 416
BstSFI CTRYAG 1 cut(s) 601
BstSLI GKGCMC 2 cut(s) 288, 354
BstV1I GCAGC 1 cut(s) 613
Bsu36I CCTNAGG 1 cut(s) 507
BtsCI GGATG 1 cut(s) 292
BveI ACCTGC 1 cut(s) 656
Cac8I GCNNGC 1 cut(s) 478
CfoI GCGC 2 cut(s) 271, 448
Cfr13I GGNCC 1 cut(s) 313
Csp6I GTAC 2 cut(s) 130, 145
CviAII CATG 3 cut(s) 310, 341, 659
CviJI RGCY 8 cut(s) 55, 60, 138, 176, 197, 250, 421, 618
CviKI_1 RGCY 8 cut(s) 55, 60, 138, 176, 197, 250, 421, 618
CviQI GTAC 2 cut(s) 130, 145
DdeI CTNAG 3 cut(s) 56, 96, 507
Eam1104I CTCTTC 1 cut(s) 33
EarI CTCTTC 1 cut(s) 33
Ecl136II GAGCTC 1 cut(s) 55
Eco24I GRGCYC 1 cut(s) 57
Eco31I GGTCTC 1 cut(s) 66
Eco32I GATATC 1 cut(s) 211
Eco47I GGWCC 1 cut(s) 313
Eco53kI GAGCTC 1 cut(s) 55
Eco81I CCTNAGG 1 cut(s) 507
EcoICRI GAGCTC 1 cut(s) 55
EcoRII CCWGG 2 cut(s) 132, 416
EcoRV GATATC 1 cut(s) 211
EcoT22I ATGCAT 2 cut(s) 159, 676
EcoT38I GRGCYC 1 cut(s) 57
FaeI CATG 3 cut(s) 313, 344, 662
FaiI YATR 8 cut(s) 155, 159, 311, 342, 408, 515, 563, 660
FatI CATG 3 cut(s) 309, 340, 658
Fnu4HI GCNGC 1 cut(s) 627
FokI GGATG 1 cut(s) 279
FriOI GRGCYC 1 cut(s) 57
Fsp4HI GCNGC 1 cut(s) 627
FspBI CTAG 3 cut(s) 272, 443, 694
GlaI GCGC 2 cut(s) 270, 447
GluI GCNGC 1 cut(s) 627
HhaI GCGC 2 cut(s) 271, 448
Hin1II CATG 3 cut(s) 313, 344, 662
Hin6I GCGC 2 cut(s) 269, 446
HinP1I GCGC 2 cut(s) 269, 446
HinfI GANTC 2 cut(s) 388, 470
HphI GGTGA 1 cut(s) 680
Hpy166II GTNNAC 1 cut(s) 286
Hpy188III TCNNGA 3 cut(s) 200, 296, 392
Hpy8I GTNNAC 1 cut(s) 286
HpyAV CCTTC 4 cut(s) 30, 224, 364, 635
HpyCH4V TGCA 6 cut(s) 157, 286, 480, 603, 658, 674
HpyF10VI GCNNNNNNNGC 3 cut(s) 277, 632, 671
HpyF3I CTNAG 3 cut(s) 56, 96, 507
Hsp92II CATG 3 cut(s) 313, 344, 662
HspAI GCGC 2 cut(s) 269, 446
KpnI GGTACC 1 cut(s) 148
LmnI GCTCC 1 cut(s) 415
Lsp1109I GCAGC 1 cut(s) 613
LweI GCATC 3 cut(s) 457, 661, 683
MaeI CTAG 3 cut(s) 272, 443, 694
MaeIII GTNAC 1 cut(s) 3
MboII GAAGA 3 cut(s) 15, 20, 593
MhlI GDGCHC 3 cut(s) 57, 288, 354
MluCI AATT 3 cut(s) 47, 258, 571
MlyI GAGTC 1 cut(s) 464
MnlI CCTC 6 cut(s) 21, 187, 348, 412, 423, 690
Mph1103I ATGCAT 2 cut(s) 159, 676
MroXI GAANNNNTTC 1 cut(s) 571
MseI TTAA 1 cut(s) 465
MspR9I CCNGG 2 cut(s) 134, 418
MvaI CCWGG 2 cut(s) 134, 418
MwoI GCNNNNNNNGC 3 cut(s) 277, 632, 671
NlaIII CATG 3 cut(s) 313, 344, 662
NlaIV GGNNCC 3 cut(s) 146, 241, 568
NmuCI GTSAC 1 cut(s) 3
NsiI ATGCAT 2 cut(s) 159, 676
NspV TTCGAA 1 cut(s) 591
PaqCI CACCTGC 1 cut(s) 656
PdmI GAANNNNTTC 1 cut(s) 571
PfeI GAWTC 1 cut(s) 388
PkrI GCNGC 1 cut(s) 628
PleI GAGTC 1 cut(s) 464
PpsI GAGTC 1 cut(s) 464
Psp124BI GAGCTC 1 cut(s) 57
Psp6I CCWGG 2 cut(s) 132, 416
PspGI CCWGG 2 cut(s) 132, 416
PspN4I GGNNCC 3 cut(s) 146, 241, 568
PspPI GGNCC 1 cut(s) 313
PstI CTGCAG 1 cut(s) 605
RsaI GTAC 2 cut(s) 131, 146
RsaNI GTAC 2 cut(s) 130, 145
SacI GAGCTC 1 cut(s) 57
SaqAI TTAA 1 cut(s) 465
SatI GCNGC 1 cut(s) 627
Sau96I GGNCC 1 cut(s) 313
SchI GAGTC 1 cut(s) 464
ScrFI CCNGG 2 cut(s) 134, 418
SduI GDGCHC 3 cut(s) 57, 288, 354
SetI ASST 8 cut(s) 13, 57, 78, 118, 199, 404, 486, 670
SfaNI GCATC 3 cut(s) 457, 661, 683
SfcI CTRYAG 1 cut(s) 601
SfuI TTCGAA 1 cut(s) 591
SinI GGWCC 1 cut(s) 313
SmlI CTYRAG 2 cut(s) 198, 251
SmoI CTYRAG 2 cut(s) 198, 251
Sse9I AATT 3 cut(s) 47, 258, 571
SspMI CTAG 3 cut(s) 272, 443, 694
SstI GAGCTC 1 cut(s) 57
StyD4I CCNGG 2 cut(s) 132, 416
TaqI TCGA 1 cut(s) 591
TasI AATT 3 cut(s) 47, 258, 571
TfiI GAWTC 1 cut(s) 388
Tru1I TTAA 1 cut(s) 465
Tru9I TTAA 1 cut(s) 465
TseFI GTSAC 1 cut(s) 3
TseI GCWGC 1 cut(s) 626
Tsp45I GTSAC 1 cut(s) 3
TspDTI ATGAA 1 cut(s) 675
VneI GTGCAC 1 cut(s) 284
VpaK11BI GGWCC 1 cut(s) 313
XmnI GAANNNNTTC 1 cut(s) 571
XspI CTAG 3 cut(s) 272, 443, 694
Zsp2I ATGCAT 2 cut(s) 159, 676
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.