FvH4_4g37370

May participate in a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0005757
Physical Location & Seq
Forward (+)
33861889 .. 33869307
7419 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g37370.t2

Sequence Viewer

Length: 2391 bp
ATGACTACTAAGCGCGCTTACAAATTACAGGAATTCGTTGCCCACACTTCCGCCGTCAATTGCCTCAAGATTGGCCGGAAAACTTCCAGGGTTCTTGTTACTGGCGGAGAAGATCACAAGGTCAATCTGTGGGCCATTGGCAAACCCAATGCCTTACTGAGCTTGTCTGGACACACCAGTGGCATTGATTCCGTCAGCTTTGATTCTTCCGAGGTCTTAGTTGCCGCAGGGGCCGCAAGTGGTACAATTAAGCTTTGGGATTTAGAAGAGGCTAAGATTGTTCGCACCCTTACTGGTCACAGATCCAATTGTATATCTGTTGATTTCCATCCTTTCGGAGAGTTCTTCGCATCTGGCTCCCTCGACACAAATTTAAAAATATGGGATATCAGGAAGAAGGGCTGTATTCACACATATAAAGGCCACACACGAGGAGTCAATGCCATCAGGTTTACCCCCGATGGACGTTGGGTTGTTTCTGGTGGAGAGGACAACACAGTGAAGTTGTGGGATCTCACTGCTGGAAAGCTCCTGCATGATTTTAAATGTCATGAGGGCCAGATACAATGTATAGACTTTCATCCCCATGAATTCCTGCTCGCAACAGGTTCGGCAGATAGGACAGTTAAATTTTGGGATCTTGAAACCTTTGAGCTTATTGGCTCAGCTGGACCTGAGACAACTGGAGTACGTTCTTTGACATTTAGTCCTGACGGGAGGGCCCTACTCTGTGGATTGCATGAAAGTTTGAAGGTATTCTCGTGGGAACCAATAAGATGCCATGATGCTGTGGATGTGGGATGGTCACGATTGTCAGATCTTAATGTTCATGAGGGTAAACTTCTTGGATGCTCCTACAATCAGAGTTGTGTCGGAGTGTGGGTTGTAGACATCTCGCGCATTGAGCCATATGCAGTCGGTAATGCTAGCAGATTGAATGGGAACTCAGAATCAAAACATTTGGCTGGTGGTAATCTGTCTGTACTCCATGAGAACGCTGCTAGGTCTAGTTTAGGCCGGCTCTCTGTTTCCCAAAGTTCAGATTCTCTAGTGAAGGAAACAAAGTCTCTTGGAAGGTTATCAGTGTCCCAAAATTCAGATCCCGCCAAGGAGCCAAAATCGTTGACATCCACTGGCAGTGTTCCTGGTACTCCTCAACGGATCAGTCTGAACTCCAGTCCAAAGGCTATTGTTTCTAGTTCAGTGTCAGTTCCTAGCGCAACATCCCTGAAAAGGAATTCTGTTAAGACCAGCGTTCAAACCTTTAACAAGTCAGATGTCATACCTGTGATTGTGCCTAGAACTAGTGCAAGGTCAGAGCTGGCAGCCGAGTCCAGAAAAGAAGCCAGTGTTGCTGGCAGAACAATGCCATTCTCTTCACAGTCAAAGACAAATGATTTTCGAAAGTTCTCCAATGCCAGAGAAGAAGTGGATAGGCCAACTATCTCTATAGTGTCTGAATCGATTGCTACTAAGGCTACCGAGTTAAGCACTACTGTAGATAGGAGCGGTTTCTCAAAAACTATAAGCTCAAGCCAAGCAATAGATAGTGCTGAAAGAAATATGAAGGATGACAGATGTGTTGGACCTGGGAAGCAGGAAACAAGTTCATTGATGGAGCCATCTGCTAGCTTCCAGCATGAAAGTTCTGAAACTCGAGGGCATAAGGTGATGAGAGATGCATACTCTGCTGAAGGTCAAAGAGGAGGACGAATGCGGTCAATTGCTGTGAATTGGGAGAGAAGAGATAGATATTCTAACTATGAGGGACCTACCTCCGATACCACCCTGGGATCAGCATCTGCAGTGAACCCTTTGAATGCGCAGAGAGGCTACACTACATCTACAAAAAAAGAAACGGTATCTACTAGTGATGAGGATGCAATTGCTGATGTTTTGGAGAAACATGACCAATTTGTTAGCTCCATGCAATCTCGTTCAACCAAGTTACAGGTAGTCTATAGATACTGGCAGAGAAATGATGTTAAGGGGGTAATTGGTGCAATGGAGAAGATGTCGGATCATGCTGTCATTGCTGATGTAGTTAGCATTATGGCGGACAAAATGGACATTGTCACCCTGGATGTATGCACTTGTCTTCTGCCACTCCTCACTGGCCTTCTAGAAAGCAACATGGATAGGCATTTAGGCATTTCTCTAGAAATGCTGCTCAAGCTGGTTAGAATATTCGGTTCTGTGATCTATTCAACATTATCAGCATCATCATCTGTTGGTGTAGATATTGAAGCCGAGCAAAGGCTGGAGCGTTGCAACTTGTGTTTCATGGAGCTTGAAAAGGTCAAAGGCTGTTTGCCTGCTCTTACCAGAAGAGGAGGATCAATTGCAAAGTCTGCACAGGAGTTGAATCTAGCTCTTCAAGAAGTTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0031461 GO:0032991 GO:0044424 GO:0044464 GO:0080008 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Katanin_con80 WD40

Protein Analysis

797

Amino Acids

86.62

Weight (kDa)

8.24

Isoelectric Point (pI)

34.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR36-Utp21_1st PF25171 4 - 128 4.5e-08 WDR36/Utp21 first beta-propeller
WD40_MABP1-WDR62_2nd PF24782 7 - 172 4.7e-12 MABP1/WDR62 second WD40 domain
Beta-prop_THOC3 PF25174 7 - 77 6.1e-07 THOC3 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 9 - 130 2.6e-11 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 10 - 137 8.4e-26 WDR5 beta-propeller domain
WD40_Gbeta PF25391 10 - 133 1.4e-14 G protein beta WD-40 repeat protein
Beta-prop_WDR3_1st PF25173 10 - 130 1.9e-23 WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 13 - 136 4.2e-13 WDR36/Utp21 second beta-propeller domain
Beta-prop_SCAP PF24017 15 - 107 1.3e-08 SCAP Beta-propeller
WD40_WDHD1_1st PF24817 15 - 97 1.7e-10 WDHD1 first WD40 domain
WD40_Prp19 PF24814 16 - 255 5.9e-47 Prp19 WD40 domain
Beta-prop_EML PF23409 16 - 173 4.8e-09 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 28 - 133 1.5e-07 WDR90/POC16, second beta-propeller
Beta-prop_EML_2 PF23414 28 - 157 2.2e-13 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 34 - 170 4.1e-13 WDR36/Utp21 first beta-propeller
WD40_CDC20-Fz PF24807 50 - 250 9.5e-34 CDC20/Fizzy WD40 domain
WD40 PF00400 52 - 87 3e-06 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 52 - 101 2.3e-07 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 52 - 174 8.1e-18 WDR3 second beta-propeller domain
EIF3I PF24805 53 - 139 6.7e-06 EIF3I
Beta-prop_CAF1B_HIR1 PF24105 54 - 138 5.2e-06 CAF1B/HIR1 beta-propeller domain
WDR55 PF24796 62 - 258 2.3e-21 WDR55
Beta-prop_THOC3 PF25174 63 - 184 3e-29 THOC3 beta-propeller domain
Beta-prop_EIPR1 PF23609 67 - 170 1e-06 EIPR1 beta-propeller
WD40_WDHD1_1st PF24817 72 - 141 1.7e-12 WDHD1 first WD40 domain
WD40 PF00400 92 - 129 3.1e-09 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 96 - 256 1.3e-36 WDR5 beta-propeller domain
Beta-prop_Aladin PF25460 102 - 190 3e-06 Aladin seven-bladed propeller
Beta-prop_WDR3_1st PF25173 114 - 253 4.4e-30 WDR3 first beta-propeller domain
Beta-prop_SCAP PF24017 118 - 193 5.8e-06 SCAP Beta-propeller
WD40_Gbeta PF25391 121 - 261 1e-17 G protein beta WD-40 repeat protein
Beta-prop_WDR36-Utp21_2nd PF25168 129 - 182 9.1e-09 WDR36/Utp21 second beta-propeller domain
WD40_MABP1-WDR62_1st PF24780 130 - 241 1.1e-07 MABP1/WDR62 first WD40 domain
Beta-prop_SPT8 PF23798 130 - 197 1e-05 SPT8 beta propeller
WD40 PF00400 135 - 171 1.1e-13 WD domain, G-beta repeat
Beta-prop_TEP1_2nd PF25047 137 - 218 1.3e-07 TEP-1 second beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 137 - 218 4e-11 CAF1B/HIR1 beta-propeller domain
EIF3I PF24805 140 - 248 2.8e-16 EIF3I
ANAPC4_WD40 PF12894 142 - 194 2e-06 Anaphase-promoting complex subunit 4 WD40 domain
WD40_MABP1-WDR62_2nd PF24782 143 - 215 3.6e-07 MABP1/WDR62 second WD40 domain
WD40_WDHD1_1st PF24817 144 - 218 2.1e-11 WDHD1 first WD40 domain
WDR90_beta-prop_4th PF23342 146 - 252 7e-06 WDR90, 4th beta-propeller
Beta-prop_EML_2 PF23414 153 - 249 4.5e-11 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 163 - 244 5.5e-11 WDR90/POC16, second beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 167 - 255 1.5e-06 WDR36/Utp21 first beta-propeller
Beta-prop_WDR3_2nd PF25172 167 - 218 9.5e-07 WDR3 second beta-propeller domain
Beta-prop_THOC3 PF25174 170 - 245 2.3e-14 THOC3 beta-propeller domain
WD40 PF00400 175 - 213 9.8e-10 WD domain, G-beta repeat
Beta-prop_WDR36-Utp21_2nd PF25168 178 - 261 1.4e-06 WDR36/Utp21 second beta-propeller domain
Beta-prop_EML_2 PF23414 190 - 295 5.4e-07 Echinoderm microtubule-associated protein second beta-propeller
Katanin_con80 PF13925 634 - 790 6.8e-55 con80 domain of Katanin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1824
AccBSI CCGCTC 1 cut(s) 1509
AccI GTMKAC 1 cut(s) 888
AccII CGCG 2 cut(s) 15, 898
AciI CCGC 8 cut(s) 51, 105, 225, 234, 1104, 1509, 1717, 2057
AclWI GGATC 8 cut(s) 297, 519, 645, 1094, 1169, 1801, 2028, 2344
AcoI YGGCCR 1 cut(s) 73
AcsI RAATTY 6 cut(s) 32, 370, 590, 629, 1093, 1237
AcuI CTGAAG 1 cut(s) 1713
AfaI GTAC 4 cut(s) 244, 690, 984, 1150
AfiI CCNNNNNNNGG 2 cut(s) 1233, 2256
AhdI GACNNNNNGTC 1 cut(s) 705
AhlI ACTAGT 2 cut(s) 1304, 1868
AjnI CCWGG 5 cut(s) 86, 1144, 1588, 1788, 2079
AleI CACNNNNGTG 1 cut(s) 177
Alw26I GTCTC 2 cut(s) 671, 1071
AlwI GGATC 8 cut(s) 297, 519, 645, 1094, 1169, 1801, 2028, 2344
AlwNI CAGNNNCTG 1 cut(s) 1802
Ama87I CYCGRG 1 cut(s) 1656
AoxI GGCC 9 cut(s) 73, 132, 231, 421, 556, 720, 1015, 1436, 2116
ApaI GGGCCC 1 cut(s) 724
ApeKI GCWGC 3 cut(s) 998, 1325, 2167
ApoI RAATTY 6 cut(s) 32, 370, 590, 629, 1093, 1237
Asp700I GAANNNNTTC 1 cut(s) 755
AspLEI GCGC 5 cut(s) 15, 17, 900, 1220, 1825
AspS9I GGNCC 8 cut(s) 132, 231, 556, 671, 720, 721, 1586, 1769
AsuHPI GGTGA 2 cut(s) 1681, 2068
AsuII TTCGAA 1 cut(s) 1402
AsuNHI GCTAGC 2 cut(s) 926, 1628
AvaI CYCGRG 1 cut(s) 1656
AvaII GGWCC 3 cut(s) 671, 1586, 1769
BaeGI GKGCMC 1 cut(s) 724
BanII GRGCYC 1 cut(s) 724
BauI CACGAG 2 cut(s) 429, 760
BbsI GAAGAC 1 cut(s) 2090
BbvI GCAGC 3 cut(s) 985, 1337, 2154
BccI CCATC 6 cut(s) 336, 452, 455, 795, 1609, 1630
BceAI ACGGC 1 cut(s) 38
BcgI CGANNNNNNTGC 2 cut(s) 591, 625
BciT130I CCWGG 5 cut(s) 88, 1146, 1590, 1790, 2081
BcoDI GTCTC 2 cut(s) 671, 1071
BcuI ACTAGT 2 cut(s) 1304, 1868
BfmI CTRYAG 4 cut(s) 1449, 1497, 1803, 1960
BglI GCCNNNNNGGC 1 cut(s) 230
BglII AGATCT 1 cut(s) 817
BisI GCNGC 5 cut(s) 225, 234, 999, 1326, 2168
BlpI GCTNAGC 1 cut(s) 664
BlsI GCNGC 5 cut(s) 226, 235, 1000, 1327, 2169
Bme1390I CCNGG 5 cut(s) 88, 1146, 1590, 1790, 2081
Bme18I GGWCC 3 cut(s) 671, 1586, 1769
BmeRI GACNNNNNGTC 1 cut(s) 705
BmeT110I CYCGRG 1 cut(s) 1656
BmgT120I GGNCC 8 cut(s) 132, 231, 556, 671, 720, 721, 1586, 1769
BmiI GGNNCC 7 cut(s) 232, 358, 722, 768, 1113, 1620, 1770
BmrFI CCNGG 5 cut(s) 88, 1146, 1590, 1790, 2081
BmsI GCATC 8 cut(s) 359, 767, 775, 839, 1669, 1808, 1870, 2228
BmtI GCTAGC 2 cut(s) 930, 1632
BpiI GAAGAC 1 cut(s) 2090
BpmI CTGGAG 3 cut(s) 705, 1159, 2282
Bpu1102I GCTNAGC 1 cut(s) 664
Bpu14I TTCGAA 1 cut(s) 1402
BpuEI CTTGAG 3 cut(s) 50, 1516, 2156
Bsa29I ATCGAT 1 cut(s) 1463
BsaBI GATNNNNATC 2 cut(s) 327, 1798
BsaJI CCNNGG 7 cut(s) 87, 210, 1107, 1589, 1788, 1789, 2079
BsaXI ACNNNNNCTCC 4 cut(s) 867, 897, 2000, 2030
Bsc4I CCNNNNNNNGG 2 cut(s) 1233, 2256
Bse118I RCCGGY 1 cut(s) 1017
Bse1I ACTGG 9 cut(s) 106, 177, 298, 688, 1138, 1176, 1347, 1973, 2119
Bse3DI GCAATG 2 cut(s) 2010, 2031
Bse8I GATNNNNATC 2 cut(s) 327, 1798
BseBI CCWGG 5 cut(s) 88, 1146, 1590, 1790, 2081
BseCI ATCGAT 1 cut(s) 1463
BseDI CCNNGG 7 cut(s) 87, 210, 1107, 1589, 1788, 1789, 2079
BseJI GATNNNNATC 2 cut(s) 327, 1798
BseLI CCNNNNNNNGG 2 cut(s) 1233, 2256
BseMI GCAATG 2 cut(s) 2010, 2031
BseMII CTCAG 4 cut(s) 149, 666, 678, 960
BseNI ACTGG 9 cut(s) 106, 177, 298, 688, 1138, 1176, 1347, 1973, 2119
BsePI GCGCGC 1 cut(s) 13
BseRI GAGGAG 5 cut(s) 447, 1143, 1719, 2099, 2346
BseSI GKGCMC 1 cut(s) 724
BseXI GCAGC 3 cut(s) 985, 1337, 2154
BsgI GTGCAG 1 cut(s) 2337
Bsh1236I CGCG 2 cut(s) 15, 898
BshFI GGCC 9 cut(s) 75, 134, 233, 423, 558, 722, 1017, 1438, 2118
BshVI ATCGAT 1 cut(s) 1463
BsiHKCI CYCGRG 1 cut(s) 1656
BsiSI CCGG 2 cut(s) 76, 1018
BslFI GGGAC 2 cut(s) 1072, 1782
BslI CCNNNNNNNGG 2 cut(s) 1233, 2256
BsmAI GTCTC 2 cut(s) 671, 1071
BsmFI GGGAC 2 cut(s) 1072, 1782
BsmI GAATGC 2 cut(s) 1719, 1825
BsnI GGCC 9 cut(s) 75, 134, 233, 423, 558, 722, 1017, 1438, 2118
BsoBI CYCGRG 1 cut(s) 1656
Bsp119I TTCGAA 1 cut(s) 1402
Bsp120I GGGCCC 1 cut(s) 720
Bsp1286I GDGCHC 1 cut(s) 724
Bsp1720I GCTNAGC 1 cut(s) 664
BspACI CCGC 8 cut(s) 51, 105, 225, 234, 1104, 1509, 1717, 2057
BspANI GGCC 9 cut(s) 75, 134, 233, 423, 558, 722, 1017, 1438, 2118
BspCNI CTCAG 4 cut(s) 150, 667, 677, 959
BspDI ATCGAT 1 cut(s) 1463
BspFNI CGCG 2 cut(s) 15, 898
BspHI TCATGA 3 cut(s) 550, 829, 2387
BspLI GGNNCC 7 cut(s) 232, 358, 722, 768, 1113, 1620, 1770
BspMAI CTGCAG 1 cut(s) 1807
BspOI GCTAGC 2 cut(s) 930, 1632
BspPI GGATC 8 cut(s) 297, 519, 645, 1094, 1169, 1801, 2028, 2344
BspQI GCTCTTC 1 cut(s) 2379
BspT104I TTCGAA 1 cut(s) 1402
BsrBI CCGCTC 1 cut(s) 1509
BsrDI GCAATG 2 cut(s) 2010, 2031
BsrFI RCCGGY 1 cut(s) 1017
BsrI ACTGG 9 cut(s) 106, 177, 298, 688, 1138, 1176, 1347, 1973, 2119
BssAI RCCGGY 1 cut(s) 1017
BssECI CCNNGG 7 cut(s) 87, 210, 1107, 1589, 1788, 1789, 2079
BssHII GCGCGC 1 cut(s) 13
BssSI CACGAG 2 cut(s) 429, 760
BssT1I CCWWGG 1 cut(s) 1107
Bst2BI CACGAG 2 cut(s) 429, 760
Bst2UI CCWGG 5 cut(s) 88, 1146, 1590, 1790, 2081
Bst4CI ACNGT 5 cut(s) 499, 625, 1383, 1498, 1861
Bst6I CTCTTC 5 cut(s) 261, 1381, 1738, 2323, 2379
BstAPI GCANNNNNTGC 2 cut(s) 1688, 2351
BstBI TTCGAA 1 cut(s) 1402
BstC8I GCNNGC 8 cut(s) 15, 600, 928, 1019, 1323, 1357, 1630, 2316
BstDEI CTNAG 8 cut(s) 9, 158, 217, 273, 664, 675, 946, 1473
BstFNI CGCG 2 cut(s) 15, 898
BstHHI GCGC 5 cut(s) 15, 17, 900, 1220, 1825
BstMAI GTCTC 2 cut(s) 671, 1071
BstMWI GCNNNNNNNGC 9 cut(s) 230, 233, 904, 1352, 1475, 1688, 2033, 2173, 2351
BstNI CCWGG 5 cut(s) 88, 1146, 1590, 1790, 2081
BstSCI CCNGG 5 cut(s) 86, 1144, 1588, 1788, 2079
BstSFI CTRYAG 4 cut(s) 1449, 1497, 1803, 1960
BstSLI GKGCMC 1 cut(s) 724
BstUI CGCG 2 cut(s) 15, 898
BstV1I GCAGC 3 cut(s) 985, 1337, 2154
BstV2I GAAGAC 1 cut(s) 2090
BstX2I RGATCY 5 cut(s) 302, 511, 637, 817, 1099
BstYI RGATCY 5 cut(s) 302, 511, 637, 817, 1099
Bsu15I ATCGAT 1 cut(s) 1463
BsuRI GGCC 9 cut(s) 75, 134, 233, 423, 558, 722, 1017, 1438, 2118
BsuTUI ATCGAT 1 cut(s) 1463
BtsI GCAGTG 3 cut(s) 516, 1144, 1812
Cac8I GCNNGC 8 cut(s) 15, 600, 928, 1019, 1323, 1357, 1630, 2316
CaiI CAGNNNCTG 1 cut(s) 1802
CciI TCATGA 3 cut(s) 550, 829, 2387
CfoI GCGC 5 cut(s) 15, 17, 900, 1220, 1825
Cfr10I RCCGGY 1 cut(s) 1017
Cfr13I GGNCC 8 cut(s) 132, 231, 556, 671, 720, 721, 1586, 1769
ClaI ATCGAT 1 cut(s) 1463
Csp6I GTAC 4 cut(s) 243, 689, 983, 1149
CviQI GTAC 4 cut(s) 243, 689, 983, 1149
DdeI CTNAG 8 cut(s) 9, 158, 217, 273, 664, 675, 946, 1473
DraI TTTAAA 2 cut(s) 375, 544
DriI GACNNNNNGTC 1 cut(s) 705
EaeI YGGCCR 1 cut(s) 73
Eam1104I CTCTTC 5 cut(s) 261, 1381, 1738, 2323, 2379
Eam1105I GACNNNNNGTC 1 cut(s) 705
EarI CTCTTC 5 cut(s) 261, 1381, 1738, 2323, 2379
EciI GGCGGA 3 cut(s) 40, 120, 2072
Eco130I CCWWGG 1 cut(s) 1107
Eco24I GRGCYC 1 cut(s) 724
Eco32I GATATC 1 cut(s) 388
Eco47I GGWCC 3 cut(s) 671, 1586, 1769
Eco57I CTGAAG 1 cut(s) 1713
Eco88I CYCGRG 1 cut(s) 1656
EcoO109I RGGNCCY 3 cut(s) 720, 721, 1769
EcoRI GAATTC 3 cut(s) 32, 590, 1237
EcoRII CCWGG 5 cut(s) 86, 1144, 1588, 1788, 2079
EcoRV GATATC 1 cut(s) 388
EcoT14I CCWWGG 1 cut(s) 1107
EcoT22I ATGCAT 1 cut(s) 1684
EcoT38I GRGCYC 1 cut(s) 724
ErhI CCWWGG 1 cut(s) 1107
FaqI GGGAC 2 cut(s) 1072, 1782
FauI CCCGC 1 cut(s) 1111
FauNDI CATATG 1 cut(s) 910
FblI GTMKAC 1 cut(s) 888
Fnu4HI GCNGC 5 cut(s) 225, 234, 999, 1326, 2168
FriOI GRGCYC 1 cut(s) 724
Fsp4HI GCNGC 5 cut(s) 225, 234, 999, 1326, 2168
FspI TGCGCA 1 cut(s) 1824
GlaI GCGC 5 cut(s) 14, 16, 899, 1219, 1824
GluI GCNGC 5 cut(s) 225, 234, 999, 1326, 2168
GsuI CTGGAG 3 cut(s) 705, 1159, 2282
HaeIII GGCC 9 cut(s) 75, 134, 233, 423, 558, 722, 1017, 1438, 2118
HapII CCGG 2 cut(s) 76, 1018
HhaI GCGC 5 cut(s) 15, 17, 900, 1220, 1825
Hin6I GCGC 5 cut(s) 13, 15, 898, 1218, 1823
HinP1I GCGC 5 cut(s) 13, 15, 898, 1218, 1823
HincII GTYRAC 1 cut(s) 1125
HindII GTYRAC 1 cut(s) 1125
HindIII AAGCTT 1 cut(s) 251
HinfI GANTC 8 cut(s) 188, 203, 435, 950, 1043, 1331, 1460, 2365
HpaII CCGG 2 cut(s) 76, 1018
HphI GGTGA 2 cut(s) 1681, 2068
Hpy166II GTNNAC 5 cut(s) 453, 839, 889, 1125, 1810
Hpy8I GTNNAC 5 cut(s) 453, 839, 889, 1125, 1810
HpyAV CCTTC 7 cut(s) 391, 745, 1048, 1068, 1561, 1688, 2129
HpyCH4III ACNGT 5 cut(s) 499, 625, 1383, 1498, 1861
HpyCH4IV ACGT 2 cut(s) 466, 691
HpyF10VI GCNNNNNNNGC 9 cut(s) 230, 233, 904, 1352, 1475, 1688, 2033, 2173, 2351
HpyF3I CTNAG 8 cut(s) 9, 158, 217, 273, 664, 675, 946, 1473
HpySE526I ACGT 2 cut(s) 466, 691
HspAI GCGC 5 cut(s) 13, 15, 898, 1218, 1823
KroI GCCGGC 1 cut(s) 1017
KroNI GCCGGC 1 cut(s) 1019
LguI GCTCTTC 1 cut(s) 2379
LmnI GCTCC 9 cut(s) 362, 534, 857, 1111, 1506, 1618, 1928, 2263, 2287
Lsp1109I GCAGC 3 cut(s) 985, 1337, 2154
LweI GCATC 8 cut(s) 359, 767, 775, 839, 1669, 1808, 1870, 2228
MaeII ACGT 2 cut(s) 466, 691
MaeIII GTNAC 5 cut(s) 97, 296, 804, 1947, 2074
MbiI CCGCTC 1 cut(s) 1509
MfeI CAATTG 5 cut(s) 58, 307, 1722, 1884, 2340
MflI RGATCY 5 cut(s) 302, 511, 637, 817, 1099
MhlI GDGCHC 1 cut(s) 724
MlyI GAGTC 2 cut(s) 444, 1340
MmeI TCCRAC 3 cut(s) 853, 1564, 1998
Mph1103I ATGCAT 1 cut(s) 1684
MroNI GCCGGC 1 cut(s) 1017
MroXI GAANNNNTTC 1 cut(s) 755
MseI TTAA 9 cut(s) 249, 374, 543, 627, 822, 1245, 1266, 1487, 1986
MslI CAYNNNNRTG 3 cut(s) 177, 585, 1286
MspA1I CMGCKG 1 cut(s) 668
MspI CCGG 2 cut(s) 76, 1018
MspR9I CCNGG 5 cut(s) 88, 1146, 1590, 1790, 2081
MunI CAATTG 5 cut(s) 58, 307, 1722, 1884, 2340
Mva1269I GAATGC 2 cut(s) 1719, 1825
MvaI CCWGG 5 cut(s) 88, 1146, 1590, 1790, 2081
MvnI CGCG 2 cut(s) 15, 898
MwoI GCNNNNNNNGC 9 cut(s) 230, 233, 904, 1352, 1475, 1688, 2033, 2173, 2351
NaeI GCCGGC 1 cut(s) 1019
NdeI CATATG 1 cut(s) 910
NgoMIV GCCGGC 1 cut(s) 1017
NheI GCTAGC 2 cut(s) 926, 1628
NlaIV GGNNCC 7 cut(s) 232, 358, 722, 768, 1113, 1620, 1770
NmeAIII GCCGAG 2 cut(s) 1354, 2275
NmuCI GTSAC 3 cut(s) 296, 804, 2074
NsbI TGCGCA 1 cut(s) 1824
NsiI ATGCAT 1 cut(s) 1684
NspV TTCGAA 1 cut(s) 1402
OliI CACNNNNGTG 1 cut(s) 177
PaeR7I CTCGAG 1 cut(s) 1656
PagI TCATGA 3 cut(s) 550, 829, 2387
PasI CCCWGGG 1 cut(s) 1789
PauI GCGCGC 1 cut(s) 13
PciSI GCTCTTC 1 cut(s) 2379
PctI GAATGC 2 cut(s) 1719, 1825
PdiI GCCGGC 1 cut(s) 1019
PdmI GAANNNNTTC 1 cut(s) 755
PfeI GAWTC 6 cut(s) 188, 203, 950, 1043, 1460, 2365
PflFI GACNNNGTC 1 cut(s) 2072
PkrI GCNGC 5 cut(s) 226, 235, 1000, 1327, 2169
PleI GAGTC 2 cut(s) 443, 1339
PpsI GAGTC 2 cut(s) 443, 1339
PpuMI RGGWCCY 1 cut(s) 1769
Psp5II RGGWCCY 1 cut(s) 1769
Psp6I CCWGG 5 cut(s) 86, 1144, 1588, 1788, 2079
PspGI CCWGG 5 cut(s) 86, 1144, 1588, 1788, 2079
PspN4I GGNNCC 7 cut(s) 232, 358, 722, 768, 1113, 1620, 1770
PspOMI GGGCCC 1 cut(s) 720
PspPI GGNCC 8 cut(s) 132, 231, 556, 671, 720, 721, 1586, 1769
PspPPI RGGWCCY 1 cut(s) 1769
PspXI VCTCGAGB 1 cut(s) 1656
PstI CTGCAG 1 cut(s) 1807
PstNI CAGNNNCTG 1 cut(s) 1802
PsuI RGATCY 5 cut(s) 302, 511, 637, 817, 1099
PsyI GACNNNGTC 1 cut(s) 2072
PteI GCGCGC 1 cut(s) 13
PvuII CAGCTG 1 cut(s) 668
RsaI GTAC 4 cut(s) 244, 690, 984, 1150
RsaNI GTAC 4 cut(s) 243, 689, 983, 1149
RseI CAYNNNNRTG 3 cut(s) 177, 585, 1286
SapI GCTCTTC 1 cut(s) 2379
SaqAI TTAA 9 cut(s) 249, 374, 543, 627, 822, 1245, 1266, 1487, 1986
SatI GCNGC 5 cut(s) 225, 234, 999, 1326, 2168
Sau96I GGNCC 8 cut(s) 132, 231, 556, 671, 720, 721, 1586, 1769
SchI GAGTC 2 cut(s) 444, 1340
ScrFI CCNGG 5 cut(s) 88, 1146, 1590, 1790, 2081
SduI GDGCHC 1 cut(s) 724
SfaNI GCATC 8 cut(s) 359, 767, 775, 839, 1669, 1808, 1870, 2228
SfcI CTRYAG 4 cut(s) 1449, 1497, 1803, 1960
Sfr274I CTCGAG 1 cut(s) 1656
SfuI TTCGAA 1 cut(s) 1402
SinI GGWCC 3 cut(s) 671, 1586, 1769
SlaI CTCGAG 1 cut(s) 1656
SmiMI CAYNNNNRTG 3 cut(s) 177, 585, 1286
SmlI CTYRAG 4 cut(s) 65, 1531, 1656, 2171
SmoI CTYRAG 4 cut(s) 65, 1531, 1656, 2171
SpeI ACTAGT 2 cut(s) 1304, 1868
SsiI CCGC 8 cut(s) 51, 105, 225, 234, 1104, 1509, 1717, 2057
SspI AATATT 1 cut(s) 2187
StyD4I CCNGG 5 cut(s) 86, 1144, 1588, 1788, 2079
StyI CCWWGG 1 cut(s) 1107
TaaI ACNGT 5 cut(s) 499, 625, 1383, 1498, 1861
TaiI ACGT 2 cut(s) 469, 694
TaqI TCGA 4 cut(s) 363, 1402, 1463, 1657
TatI WGTACW 1 cut(s) 982
TauI GCSGC 2 cut(s) 227, 236
TfiI GAWTC 6 cut(s) 188, 203, 950, 1043, 1460, 2365
Tru1I TTAA 9 cut(s) 249, 374, 543, 627, 822, 1245, 1266, 1487, 1986
Tru9I TTAA 9 cut(s) 249, 374, 543, 627, 822, 1245, 1266, 1487, 1986
TseFI GTSAC 3 cut(s) 296, 804, 2074
TseI GCWGC 3 cut(s) 998, 1325, 2167
Tsp45I GTSAC 3 cut(s) 296, 804, 2074
TspDTI ATGAA 9 cut(s) 569, 603, 756, 818, 1580, 1599, 1656, 2272, 2376
TspGWI ACGGA 2 cut(s) 181, 1174
Tth111I GACNNNGTC 1 cut(s) 2072
VpaK11BI GGWCC 3 cut(s) 671, 1586, 1769
XapI RAATTY 6 cut(s) 32, 370, 590, 629, 1093, 1237
XbaI TCTAGA 2 cut(s) 2122, 2158
XcmI CCANNNNNNNNNTGG 1 cut(s) 1426
XhoI CTCGAG 1 cut(s) 1656
XmiI GTMKAC 1 cut(s) 888
XmnI GAANNNNTTC 1 cut(s) 755
Zsp2I ATGCAT 1 cut(s) 1684
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.