MD13G1000300.v1.1

May participate in a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0005757
Physical Location & Seq
Reverse (-)
31554 .. 41940
10387 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1000300.v1.1.491

Sequence Viewer

Length: 2412 bp
ATGACTACTAAACGCGCTTACAAGCTACAGGAATTTGTAGCACATTCTGCAGCTGTGAATTGCCTCAAGATTGGCAGAAAATCTTCGAGAGTTCTTGTCACCGGCGGAGAAGATCACAAGGTCAATCTCTGGGCCATTGGGAAACCCAATGCCTTACTGAGTTTGTCTGGACACACCAGTGGAATTGATTCAGTAAGCTTTGATTCTTCAGAGGTCTTAGTAGCTGCAGGAGCTGCAAGTGGGACAATTAAGCTTTGGGATTTAGAGGAGGCAAAGATTGTTCGCACACTTACCGGTCACAGATCCAATTGTATATCTGTGGACTTTCATCCTTTTGGGGAGTTCTTTGCATCTGGCTCCTTAGACACAAATCTCAAAATATGGGATATCAGGAAGAAGGGGTGTATTCACACATACAAAGGCCATACACGAGGGGTCAATGCTATCAGATTTACCCCGGATGGCCGCTGGGTTGTTTCTTGTGGAGAGGACAACACTGTGAAGTTGTGGGACCTGACTGCTGGAAAGATGCTGCATGATTTCAAGTGTCATGAGGGCCAAGTTCAGTGCATAGACTTTCATCCCCATGAGTTTCTGCTAGCAACAGGTTCAGCGGATAGGACAGTCAAATTTTGGGACCTCGAAACCTTTGAGCTAATTGGCTCAGCTGGACCTGAGACAACTGGAGTGCGTTCTTTGACTTTTAGCCCGGATGGGAGAGCCCTACTCTGTGGATTGCATGAAAGTTTAAAGGTTTTCTCGTGGGAGCCAATAAGATGCCATGATGTTGTGGAGGTGGGATGGTCTCGATTGTCAGATCTTACTGTTCATGAGGGAAAGCTTCTTGGATGCTCATACAATCAGAGTTGTGTTGGAGTGTGGGTTGTAGACATCTTGCGCATAGAACCGTATGCAATTGGTAATGCCAGCAGATTGAGTGGGCACTCAGAATCTAAATCATTGTCTGGTGGGAATCTCTCCGTGCTCAATGAAAATGCTGCTAAATCTAGTTTGGGCCGGTTATCTATTTCTCACAATTCAGATTCTTTAGTGAAGGAAACAAAGTCTCTTGGAAGGTTATCAGTTTCTCAGAATTCAGATCTCGTTAAGGAGCCAAAAATTATTGCATCCACAGGAAGTTTTCCTGTTACTCCTCAAAGGATCAATTTGAATTCCAGTCCAAAAACTATTGCGGCTAGTTCAGTGGCAGTTCCTACTGTAACAACCCTGAAGAGGAGTTCTACTAAGACCCATTCAACAGCTAATATTCCAACATTTAACAAGTCTGATGTCATACCTGTAATTGTCCCAAGAACTAGTATGAGGTCGGAGCTGTCAGCTGAGTCTAGAAAAGAGGTCGGCATTGCTGGAAGGCCACTGGCGTTTTCTTTGCAGTCAAAGGCAACTGATCTCCGGAAGTTGTCCAATAGCAGAGAAGAAGTGGATAAGCCAGCTGTGTCTTCAGTGTCTGAATCCCCTGCTTCTAAGGCTATTGAATTAAGCAGTGCGGCAGATAAGAATGGCTTCTCAACAGTTATAAATTCAACTCGGGAAATACATGCTGAAAAAAACACAAAGGATGATATATGTTTTGGGCCTGGGAAGCATGAAACAAGTTCAATGATGGAGCCGACTGCGAGTTACCAGCATGAAAGTTATGAGGCTCCAGGGCACAAGGTGAGTGGAGATGCACATTTTGCTGAAGGTCAAAGAGGAGGGAGAATGCGCTCAATTGCTGTAAATTGGGAGAAAAGAGAGAGAAATTCTAACTACGAGGGACCTACTTCCAATGGTACCATGGGAACAGCATCTGCACCAAACATTACCCCTTTAATTGCGTTTAAGCAACGAGGTTATCCTACATCTACTGAAAAAGAAACAGTGACTGCTAGTGATGAGGATGCTGTTGCAGATCTCCTGGAAAAACACAATCTATTTGTTGGCTCCATGCAATCTCGTCTTGCCAAATTACAGGTAGTCTATAGATATTGGGAAAGGAATGATGTTAAGGCGGTTCTTGGTGCAATGGAGAAGATGGCTGATCATGCTGTCATTGCAGATGTAGTTGGCATTATGACAGAAAAATTTGATATTGTCACGCTGGATATATGCACTTGTCTTCTGCCACTCCTCACTGGCCTTCTAGAAAGTAACATGGATAGGCATTTAGGCATTTCTTTGGAAATGCTGCTCAAGTTGGTTAGAGTATTTGGTTCTGTGATCTATTCAGCAATATCTGCATCATCATCTGTTGGCGTTGATATTGAAGCCGAGAAAAGGCTGGAGCGTTGCAACATCTGTTTTATGGAGCTTGAAAAGGTCAAATGCAGTTTGCCTGCTCTTACCAGAAGAGGAGGATCAATTGCAAAGTCTGCACAGGAGTTGAATTTAGCTCTTCAGGAAATTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0031461 GO:0032991 GO:0044424 GO:0044464 GO:0080008 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Katanin_con80 WD40

Protein Analysis

804

Amino Acids

87.31

Weight (kDa)

7.85

Isoelectric Point (pI)

35.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR36-Utp21_1st PF25171 6 - 128 8.2e-08 WDR36/Utp21 first beta-propeller
WD40_MABP1-WDR62_2nd PF24782 6 - 172 5.1e-13 MABP1/WDR62 second WD40 domain
Beta-prop_THOC3 PF25174 7 - 87 2.4e-08 THOC3 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 9 - 130 7.2e-11 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 10 - 138 8.4e-26 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 10 - 130 5e-23 WDR3 first beta-propeller domain
WD40_Gbeta PF25391 11 - 132 8.3e-14 G protein beta WD-40 repeat protein
Beta-prop_WDR36-Utp21_2nd PF25168 13 - 133 1.1e-12 WDR36/Utp21 second beta-propeller domain
Beta-prop_SCAP PF24017 15 - 107 1.3e-08 SCAP Beta-propeller
WD40_Prp19 PF24814 15 - 255 1.7e-46 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 15 - 97 1.8e-10 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 28 - 158 1.2e-13 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 28 - 124 7.5e-07 WDR90/POC16, second beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 34 - 170 2.5e-13 WDR36/Utp21 first beta-propeller
WD40_CDC20-Fz PF24807 50 - 253 1.2e-33 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_2nd PF25172 52 - 174 3.2e-18 WDR3 second beta-propeller domain
WD40 PF00400 52 - 87 3e-06 WD domain, G-beta repeat
EIF3I PF24805 53 - 139 9.3e-06 EIF3I
Beta-prop_CAF1B_HIR1 PF24105 54 - 138 2.6e-06 CAF1B/HIR1 beta-propeller domain
Beta-prop_EML PF23409 57 - 177 1.5e-08 Echinoderm microtubule-associated protein first beta-propeller
WDR55 PF24796 62 - 257 2.9e-20 WDR55
Beta-prop_EIPR1 PF23609 67 - 170 1.1e-07 EIPR1 beta-propeller
Beta-prop_THOC3 PF25174 68 - 184 8.4e-28 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 73 - 141 4.5e-12 WDHD1 first WD40 domain
WD40 PF00400 92 - 129 3.1e-09 WD domain, G-beta repeat
Beta-prop_TEP1_2nd PF25047 96 - 184 2e-08 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 96 - 257 1.1e-37 WDR5 beta-propeller domain
Beta-prop_Aladin PF25460 102 - 190 9.4e-07 Aladin seven-bladed propeller
Beta-prop_WDR3_1st PF25173 114 - 253 7.2e-30 WDR3 first beta-propeller domain
WD40_Gbeta PF25391 121 - 261 1.2e-16 G protein beta WD-40 repeat protein
Beta-prop_WDR75_1st PF23869 124 - 181 6.8e-06 WD repeat-containing protein 75 first beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 129 - 202 1e-10 WDR36/Utp21 second beta-propeller domain
WD40_MABP1-WDR62_1st PF24780 130 - 242 1.3e-07 MABP1/WDR62 first WD40 domain
WD40 PF00400 135 - 171 1.2e-13 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 137 - 218 7.2e-11 CAF1B/HIR1 beta-propeller domain
EIF3I PF24805 140 - 248 2e-17 EIF3I
ANAPC4_WD40 PF12894 142 - 194 5.6e-06 Anaphase-promoting complex subunit 4 WD40 domain
WD40_WDHD1_1st PF24817 144 - 218 2.7e-11 WDHD1 first WD40 domain
WDR90_beta-prop_4th PF23342 146 - 253 2.8e-07 WDR90, 4th beta-propeller
NBCH_WD40 PF20426 151 - 215 4.6e-06 Neurobeachin beta propeller domain
Beta-prop_EML_2 PF23414 153 - 249 6.5e-12 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 163 - 244 2.7e-11 WDR90/POC16, second beta-propeller
Beta-prop_THOC3 PF25174 166 - 245 7.4e-16 THOC3 beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 167 - 255 1.9e-06 WDR36/Utp21 first beta-propeller
Beta-prop_WDR3_2nd PF25172 168 - 218 1.2e-06 WDR3 second beta-propeller domain
WD40 PF00400 175 - 213 1.1e-09 WD domain, G-beta repeat
Beta-prop_WDR36-Utp21_2nd PF25168 178 - 260 3.2e-06 WDR36/Utp21 second beta-propeller domain
Katanin_con80 PF13925 642 - 799 3.7e-53 con80 domain of Katanin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1538
Acc16I TGCGCA 1 cut(s) 899
Acc65I GGTACC 1 cut(s) 1793
AccB1I GGYRCC 1 cut(s) 1793
AccI GTMKAC 1 cut(s) 888
AccII CGCG 1 cut(s) 15
AccIII TCCGGA 1 cut(s) 1413
AciI CCGC 6 cut(s) 105, 466, 614, 1193, 1508, 2012
AclWI GGATC 3 cut(s) 297, 1169, 2365
AcoI YGGCCR 1 cut(s) 463
AcsI RAATTY 9 cut(s) 32, 629, 1093, 1171, 1540, 1762, 2084, 2386, 2403
AcuI CTGAAG 5 cut(s) 192, 1250, 1446, 1722, 2381
AdeI CACNNNGTG 1 cut(s) 1678
AfaI GTAC 1 cut(s) 1795
AfiI CCNNNNNNNGG 2 cut(s) 1233, 2277
AgeI ACCGGT 1 cut(s) 293
AgsI TTSAA 9 cut(s) 544, 1171, 1257, 1496, 1545, 1620, 2267, 2315, 2386
AhlI ACTAGT 1 cut(s) 1316
AjnI CCWGG 3 cut(s) 1597, 1666, 1917
AleI CACNNNNGTG 1 cut(s) 177
Alw21I GWGCWC 1 cut(s) 987
Alw26I GTCTC 3 cut(s) 671, 810, 1071
AlwI GGATC 3 cut(s) 297, 1169, 2365
AlwNI CAGNNNCTG 4 cut(s) 233, 1469, 1811, 1886
Ama87I CYCGRG 1 cut(s) 1548
Aor13HI TCCGGA 1 cut(s) 1413
AoxI GGCC 8 cut(s) 132, 421, 463, 556, 1015, 1373, 1595, 2137
ApeKI GCWGC 6 cut(s) 50, 224, 233, 532, 998, 2188
ApoI RAATTY 9 cut(s) 32, 629, 1093, 1171, 1540, 1762, 2084, 2386, 2403
AsiGI ACCGGT 1 cut(s) 293
Asp700I GAANNNNTTC 3 cut(s) 82, 187, 1523
Asp718I GGTACC 1 cut(s) 1793
AspLEI GCGC 3 cut(s) 17, 900, 1728
AspS9I GGNCC 8 cut(s) 132, 511, 556, 637, 671, 1015, 1595, 1778
AsuC2I CCSGG 2 cut(s) 458, 710
AsuHPI GGTGA 2 cut(s) 91, 1690
AsuNHI GCTAGC 1 cut(s) 598
AvaI CYCGRG 1 cut(s) 1548
AvaII GGWCC 4 cut(s) 511, 637, 671, 1778
BaeGI GKGCMC 2 cut(s) 945, 1674
BanI GGYRCC 1 cut(s) 1793
BanII GRGCYC 1 cut(s) 724
BauI CACGAG 2 cut(s) 429, 760
BbsI GAAGAC 2 cut(s) 1452, 2111
Bbv12I GWGCWC 1 cut(s) 987
BbvI GCAGC 6 cut(s) 62, 211, 220, 519, 985, 2175
BccI CCATC 5 cut(s) 455, 707, 795, 1618, 2029
BciT130I CCWGG 3 cut(s) 1599, 1668, 1919
BclI TGATCA 1 cut(s) 2041
BcnI CCSGG 2 cut(s) 458, 710
BcoDI GTCTC 3 cut(s) 671, 810, 1071
BcuI ACTAGT 1 cut(s) 1316
BfaI CTAG 7 cut(s) 599, 1008, 1197, 1317, 1347, 1890, 2144
BfmI CTRYAG 4 cut(s) 26, 48, 225, 1981
BglII AGATCT 3 cut(s) 817, 1099, 1912
BisI GCNGC 9 cut(s) 51, 225, 234, 466, 533, 999, 1194, 1509, 2189
BlpI GCTNAGC 1 cut(s) 664
BlsI GCNGC 9 cut(s) 52, 226, 235, 467, 534, 1000, 1195, 1510, 2190
Bme1390I CCNGG 5 cut(s) 458, 710, 1599, 1668, 1919
Bme18I GGWCC 4 cut(s) 511, 637, 671, 1778
BmeT110I CYCGRG 1 cut(s) 1548
BmgT120I GGNCC 8 cut(s) 132, 511, 556, 637, 671, 1015, 1595, 1778
BmrFI CCNGG 5 cut(s) 458, 710, 1599, 1668, 1919
BmsI GCATC 9 cut(s) 359, 519, 767, 839, 1136, 1678, 1817, 1891, 2249
BmtI GCTAGC 1 cut(s) 602
BpiI GAAGAC 2 cut(s) 1452, 2111
BplI GAGNNNNNCTC 2 cut(s) 711, 743
BpmI CTGGAG 3 cut(s) 705, 1650, 2303
Bpu1102I GCTNAGC 1 cut(s) 664
BpuEI CTTGAG 2 cut(s) 50, 2177
BpuMI CCSGG 2 cut(s) 458, 710
BsaI GGTCTC 1 cut(s) 810
BsaJI CCNNGG 4 cut(s) 456, 1598, 1667, 1797
BsaWI WCCGGW 2 cut(s) 293, 1413
BsaXI ACNNNNNCTCC 2 cut(s) 867, 897
Bsc4I CCNNNNNNNGG 2 cut(s) 1233, 2277
Bse118I RCCGGY 3 cut(s) 101, 293, 1017
Bse1I ACTGG 5 cut(s) 177, 688, 1176, 1383, 2140
Bse3DI GCAATG 3 cut(s) 1362, 2031, 2052
BseAI TCCGGA 1 cut(s) 1413
BseBI CCWGG 3 cut(s) 1599, 1668, 1919
BseDI CCNNGG 4 cut(s) 456, 1598, 1667, 1797
BseGI GGATG 9 cut(s) 328, 466, 580, 718, 806, 854, 1127, 1585, 1906
BseLI CCNNNNNNNGG 2 cut(s) 1233, 2277
BseMI GCAATG 3 cut(s) 1362, 2031, 2052
BseMII CTCAG 6 cut(s) 149, 666, 678, 960, 1103, 1332
BseNI ACTGG 5 cut(s) 177, 688, 1176, 1383, 2140
BseRI GAGGAG 6 cut(s) 281, 1143, 1249, 1728, 2120, 2367
BseSI GKGCMC 2 cut(s) 945, 1674
BseXI GCAGC 6 cut(s) 62, 211, 220, 519, 985, 2175
BseYI CCCAGC 1 cut(s) 468
BsgI GTGCAG 2 cut(s) 1797, 2358
Bsh1236I CGCG 1 cut(s) 15
BshFI GGCC 8 cut(s) 134, 423, 465, 558, 1017, 1375, 1597, 2139
BshNI GGYRCC 1 cut(s) 1793
BshTI ACCGGT 1 cut(s) 293
BsiHKAI GWGCWC 1 cut(s) 987
BsiHKCI CYCGRG 1 cut(s) 1548
BsiSI CCGG 6 cut(s) 102, 294, 458, 710, 1018, 1414
BslFI GGGAC 5 cut(s) 256, 524, 650, 1292, 1791
BslI CCNNNNNNNGG 2 cut(s) 1233, 2277
BsmAI GTCTC 3 cut(s) 671, 810, 1071
BsmFI GGGAC 5 cut(s) 256, 524, 650, 1292, 1791
BsmI GAATGC 1 cut(s) 1728
BsnI GGCC 8 cut(s) 134, 423, 465, 558, 1017, 1375, 1597, 2139
Bso31I GGTCTC 1 cut(s) 810
BsoBI CYCGRG 1 cut(s) 1548
Bsp1286I GDGCHC 4 cut(s) 724, 945, 987, 1674
Bsp13I TCCGGA 1 cut(s) 1413
Bsp1720I GCTNAGC 1 cut(s) 664
Bsp19I CCATGG 1 cut(s) 1797
BspACI CCGC 6 cut(s) 105, 466, 614, 1193, 1508, 2012
BspANI GGCC 8 cut(s) 134, 423, 465, 558, 1017, 1375, 1597, 2139
BspCNI CTCAG 6 cut(s) 150, 667, 677, 959, 1102, 1333
BspEI TCCGGA 1 cut(s) 1413
BspFNI CGCG 1 cut(s) 15
BspHI TCATGA 3 cut(s) 550, 829, 2408
BspMAI CTGCAG 2 cut(s) 52, 229
BspOI GCTAGC 1 cut(s) 602
BspPI GGATC 3 cut(s) 297, 1169, 2365
BspQI GCTCTTC 1 cut(s) 2400
BspT107I GGYRCC 1 cut(s) 1793
BspTNI GGTCTC 1 cut(s) 810
BsrDI GCAATG 3 cut(s) 1362, 2031, 2052
BsrFI RCCGGY 3 cut(s) 101, 293, 1017
BsrI ACTGG 5 cut(s) 177, 688, 1176, 1383, 2140
BssAI RCCGGY 3 cut(s) 101, 293, 1017
BssECI CCNNGG 4 cut(s) 456, 1598, 1667, 1797
BssSI CACGAG 2 cut(s) 429, 760
BssT1I CCWWGG 1 cut(s) 1797
Bst2BI CACGAG 2 cut(s) 429, 760
Bst2UI CCWGG 3 cut(s) 1599, 1668, 1919
Bst4CI ACNGT 7 cut(s) 499, 625, 826, 909, 1219, 1534, 1882
Bst6I CTCTTC 3 cut(s) 1226, 2344, 2400
BstAPI GCANNNNNTGC 5 cut(s) 47, 233, 1697, 2237, 2372
BstC8I GCNNGC 4 cut(s) 600, 928, 1452, 2337
BstDSI CCRYGG 1 cut(s) 1797
BstF5I GGATG 9 cut(s) 328, 466, 580, 718, 806, 854, 1127, 1585, 1906
BstFNI CGCG 1 cut(s) 15
BstHHI GCGC 3 cut(s) 17, 900, 1728
BstMAI GTCTC 3 cut(s) 671, 810, 1071
BstNI CCWGG 3 cut(s) 1599, 1668, 1919
BstNSI RCATGY 1 cut(s) 1562
BstSCI CCNGG 5 cut(s) 456, 708, 1597, 1666, 1917
BstSFI CTRYAG 4 cut(s) 26, 48, 225, 1981
BstSLI GKGCMC 2 cut(s) 945, 1674
BstUI CGCG 1 cut(s) 15
BstV1I GCAGC 6 cut(s) 62, 211, 220, 519, 985, 2175
BstV2I GAAGAC 2 cut(s) 1452, 2111
BstX2I RGATCY 4 cut(s) 302, 817, 1099, 1912
BstYI RGATCY 4 cut(s) 302, 817, 1099, 1912
BsuRI GGCC 8 cut(s) 134, 423, 465, 558, 1017, 1375, 1597, 2139
BtgI CCRYGG 1 cut(s) 1797
BtsCI GGATG 9 cut(s) 328, 466, 580, 718, 806, 854, 1127, 1585, 1906
BtsI GCAGTG 1 cut(s) 1510
BtsIMutI CAGTG 9 cut(s) 184, 495, 572, 1209, 1376, 1470, 1510, 1887, 2133
Cac8I GCNNGC 4 cut(s) 600, 928, 1452, 2337
CaiI CAGNNNCTG 4 cut(s) 233, 1469, 1811, 1886
CciI TCATGA 3 cut(s) 550, 829, 2408
CfoI GCGC 3 cut(s) 17, 900, 1728
Cfr10I RCCGGY 3 cut(s) 101, 293, 1017
Cfr13I GGNCC 8 cut(s) 132, 511, 556, 637, 671, 1015, 1595, 1778
Csp6I GTAC 1 cut(s) 1794
CspAI ACCGGT 1 cut(s) 293
CspCI CAANNNNNGTGG 2 cut(s) 1663, 1698
CviQI GTAC 1 cut(s) 1794
DraI TTTAAA 1 cut(s) 750
DraIII CACNNNGTG 1 cut(s) 1678
EaeI YGGCCR 1 cut(s) 463
Eam1104I CTCTTC 3 cut(s) 1226, 2344, 2400
EarI CTCTTC 3 cut(s) 1226, 2344, 2400
EciI GGCGGA 1 cut(s) 120
Eco130I CCWWGG 1 cut(s) 1797
Eco24I GRGCYC 1 cut(s) 724
Eco31I GGTCTC 1 cut(s) 810
Eco32I GATATC 1 cut(s) 388
Eco47I GGWCC 4 cut(s) 511, 637, 671, 1778
Eco57I CTGAAG 5 cut(s) 192, 1250, 1446, 1722, 2381
Eco88I CYCGRG 1 cut(s) 1548
EcoO109I RGGNCCY 3 cut(s) 511, 637, 1778
EcoRI GAATTC 2 cut(s) 1093, 1171
EcoRII CCWGG 3 cut(s) 1597, 1666, 1917
EcoRV GATATC 1 cut(s) 388
EcoT14I CCWWGG 1 cut(s) 1797
EcoT38I GRGCYC 1 cut(s) 724
ErhI CCWWGG 1 cut(s) 1797
FalI AAGNNNNNCTT 2 cut(s) 1508, 1540
FaqI GGGAC 5 cut(s) 256, 524, 650, 1292, 1791
FbaI TGATCA 1 cut(s) 2041
FblI GTMKAC 1 cut(s) 888
Fnu4HI GCNGC 9 cut(s) 51, 225, 234, 466, 533, 999, 1194, 1509, 2189
FokI GGATG 9 cut(s) 315, 473, 567, 725, 813, 861, 1114, 1592, 1913
FriOI GRGCYC 1 cut(s) 724
Fsp4HI GCNGC 9 cut(s) 51, 225, 234, 466, 533, 999, 1194, 1509, 2189
FspBI CTAG 7 cut(s) 599, 1008, 1197, 1317, 1347, 1890, 2144
FspI TGCGCA 1 cut(s) 899
GlaI GCGC 3 cut(s) 16, 899, 1727
GluI GCNGC 9 cut(s) 51, 225, 234, 466, 533, 999, 1194, 1509, 2189
GsaI CCCAGC 1 cut(s) 472
GsuI CTGGAG 3 cut(s) 705, 1650, 2303
HaeIII GGCC 8 cut(s) 134, 423, 465, 558, 1017, 1375, 1597, 2139
HapII CCGG 6 cut(s) 102, 294, 458, 710, 1018, 1414
HhaI GCGC 3 cut(s) 17, 900, 1728
Hin6I GCGC 3 cut(s) 15, 898, 1726
HinP1I GCGC 3 cut(s) 15, 898, 1726
HindIII AAGCTT 3 cut(s) 196, 251, 839
HinfI GANTC 7 cut(s) 188, 203, 950, 973, 1043, 1343, 1472
HpaII CCGG 6 cut(s) 102, 294, 458, 710, 1018, 1414
HphI GGTGA 2 cut(s) 91, 1690
Hpy166II GTNNAC 2 cut(s) 322, 889
Hpy8I GTNNAC 2 cut(s) 322, 889
HpyAV CCTTC 6 cut(s) 391, 1048, 1068, 1365, 1697, 2150
HpyCH4III ACNGT 7 cut(s) 499, 625, 826, 909, 1219, 1534, 1882
HspAI GCGC 3 cut(s) 15, 898, 1726
Kpn2I TCCGGA 1 cut(s) 1413
KpnI GGTACC 1 cut(s) 1797
Ksp22I TGATCA 1 cut(s) 2041
LguI GCTCTTC 1 cut(s) 2400
Lsp1109I GCAGC 6 cut(s) 62, 211, 220, 519, 985, 2175
LweI GCATC 9 cut(s) 359, 519, 767, 839, 1136, 1678, 1817, 1891, 2249
MaeI CTAG 7 cut(s) 599, 1008, 1197, 1317, 1347, 1890, 2144
MaeIII GTNAC 8 cut(s) 97, 296, 1147, 1219, 1640, 1882, 2095, 2150
MfeI CAATTG 4 cut(s) 307, 915, 1731, 2361
MflI RGATCY 4 cut(s) 302, 817, 1099, 1912
MhlI GDGCHC 4 cut(s) 724, 945, 987, 1674
MlyI GAGTC 1 cut(s) 1352
MmeI TCCRAC 3 cut(s) 853, 1295, 1308
MroI TCCGGA 1 cut(s) 1413
MroXI GAANNNNTTC 3 cut(s) 82, 187, 1523
MseI TTAA 8 cut(s) 249, 749, 1107, 1278, 1499, 1832, 1842, 2007
MslI CAYNNNNRTG 2 cut(s) 177, 585
MspA1I CMGCKG 6 cut(s) 53, 468, 614, 668, 1340, 1454
MspI CCGG 6 cut(s) 102, 294, 458, 710, 1018, 1414
MspR9I CCNGG 5 cut(s) 458, 710, 1599, 1668, 1919
MunI CAATTG 4 cut(s) 307, 915, 1731, 2361
Mva1269I GAATGC 1 cut(s) 1728
MvaI CCWGG 3 cut(s) 1599, 1668, 1919
MvnI CGCG 1 cut(s) 15
NciI CCSGG 2 cut(s) 458, 710
NcoI CCATGG 1 cut(s) 1797
NheI GCTAGC 1 cut(s) 598
NmeAIII GCCGAG 1 cut(s) 2296
NmuCI GTSAC 4 cut(s) 97, 296, 1882, 2095
NsbI TGCGCA 1 cut(s) 899
NspI RCATGY 1 cut(s) 1562
OliI CACNNNNGTG 1 cut(s) 177
PagI TCATGA 3 cut(s) 550, 829, 2408
PciSI GCTCTTC 1 cut(s) 2400
PctI GAATGC 1 cut(s) 1728
PdmI GAANNNNTTC 3 cut(s) 82, 187, 1523
PfeI GAWTC 6 cut(s) 188, 203, 950, 973, 1043, 1472
PfoI TCCNGGA 1 cut(s) 1917
PinAI ACCGGT 1 cut(s) 293
PkrI GCNGC 9 cut(s) 52, 226, 235, 467, 534, 1000, 1195, 1510, 2190
PleI GAGTC 1 cut(s) 1351
PpsI GAGTC 1 cut(s) 1351
PpuMI RGGWCCY 3 cut(s) 511, 637, 1778
PsiI TTATAA 1 cut(s) 1538
Psp5II RGGWCCY 3 cut(s) 511, 637, 1778
Psp6I CCWGG 3 cut(s) 1597, 1666, 1917
PspFI CCCAGC 1 cut(s) 468
PspGI CCWGG 3 cut(s) 1597, 1666, 1917
PspPI GGNCC 8 cut(s) 132, 511, 556, 637, 671, 1015, 1595, 1778
PspPPI RGGWCCY 3 cut(s) 511, 637, 1778
PstI CTGCAG 2 cut(s) 52, 229
PstNI CAGNNNCTG 4 cut(s) 233, 1469, 1811, 1886
PsuI RGATCY 4 cut(s) 302, 817, 1099, 1912
PvuII CAGCTG 4 cut(s) 53, 668, 1340, 1454
RsaI GTAC 1 cut(s) 1795
RsaNI GTAC 1 cut(s) 1794
RseI CAYNNNNRTG 2 cut(s) 177, 585
SapI GCTCTTC 1 cut(s) 2400
SaqAI TTAA 8 cut(s) 249, 749, 1107, 1278, 1499, 1832, 1842, 2007
SatI GCNGC 9 cut(s) 51, 225, 234, 466, 533, 999, 1194, 1509, 2189
Sau96I GGNCC 8 cut(s) 132, 511, 556, 637, 671, 1015, 1595, 1778
SchI GAGTC 1 cut(s) 1352
ScrFI CCNGG 5 cut(s) 458, 710, 1599, 1668, 1919
SduI GDGCHC 4 cut(s) 724, 945, 987, 1674
SfaNI GCATC 9 cut(s) 359, 519, 767, 839, 1136, 1678, 1817, 1891, 2249
SfcI CTRYAG 4 cut(s) 26, 48, 225, 1981
SgrAI CRCCGGYG 1 cut(s) 101
SinI GGWCC 4 cut(s) 511, 637, 671, 1778
SmiMI CAYNNNNRTG 2 cut(s) 177, 585
SmlI CTYRAG 2 cut(s) 65, 2192
SmoI CTYRAG 2 cut(s) 65, 2192
SpeI ACTAGT 1 cut(s) 1316
SsiI CCGC 6 cut(s) 105, 466, 614, 1193, 1508, 2012
SspI AATATT 1 cut(s) 1267
SspMI CTAG 7 cut(s) 599, 1008, 1197, 1317, 1347, 1890, 2144
StyD4I CCNGG 5 cut(s) 456, 708, 1597, 1666, 1917
StyI CCWWGG 1 cut(s) 1797
TaaI ACNGT 7 cut(s) 499, 625, 826, 909, 1219, 1534, 1882
TaqI TCGA 3 cut(s) 86, 642, 808
TauI GCSGC 3 cut(s) 468, 1196, 1511
TfiI GAWTC 6 cut(s) 188, 203, 950, 973, 1043, 1472
Tru1I TTAA 8 cut(s) 249, 749, 1107, 1278, 1499, 1832, 1842, 2007
Tru9I TTAA 8 cut(s) 249, 749, 1107, 1278, 1499, 1832, 1842, 2007
TscAI CASTG 9 cut(s) 184, 502, 572, 1209, 1383, 1470, 1510, 1887, 2140
TseFI GTSAC 4 cut(s) 97, 296, 1882, 2095
TseI GCWGC 6 cut(s) 50, 224, 233, 532, 998, 2188
Tsp45I GTSAC 4 cut(s) 97, 296, 1882, 2095
TspDTI ATGAA 8 cut(s) 317, 569, 756, 818, 1005, 1623, 1665, 2397
TspGWI ACGGA 1 cut(s) 970
TspRI CASTG 9 cut(s) 184, 502, 572, 1209, 1383, 1470, 1510, 1887, 2140
VpaK11BI GGWCC 4 cut(s) 511, 637, 671, 1778
XapI RAATTY 9 cut(s) 32, 629, 1093, 1171, 1540, 1762, 2084, 2386, 2403
XbaI TCTAGA 2 cut(s) 1346, 2143
XceI RCATGY 1 cut(s) 1562
XcmI CCANNNNNNNNNTGG 1 cut(s) 1795
XmiI GTMKAC 1 cut(s) 888
XmnI GAANNNNTTC 3 cut(s) 82, 187, 1523
XspI CTAG 7 cut(s) 599, 1008, 1197, 1317, 1347, 1890, 2144
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.