Rorug04G0379200

May participate in a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0005757
Physical Location & Seq
Reverse (-)
54987133 .. 54987705
573 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0379200.1

Sequence Viewer

Length: 573 bp
ATGGGGGAAGGCAAAGTGCAGTCGTCAAATGACATTCTGCTGATCATTACATGGCCTGAAGAATTTCACAAGGCTAGAATGAGTTCCACTATACCAGTCCAAAAAACTACAAGGAGATGGCAGGCTGCTGCAATTCCAAATGTTGATGGAGCCTTTATCTCCCAAGAATCATATGGTGGAACTGGAGGAGTTTTAAGAAGCTCAAGTGGAGCCTTTCAAGCTGCTTTCTCAAAGTCAAAGCCGGTTTTTTATGTAAACTCGGCACAACAGGTGGAACTGTTGGCAATCAAGGAAGGCCTGCAGTTCATCAAGAATCTGCAGATACAGATTGTCATAGTTGAAACTGATTGTCTAGTGGCAGTTCAAGAGATCACAGGTACTGCCTTGAATCTCTCAGTTACGAGCTGCATTGTCCATGATATTCAACAGGATTTGGGTACCATGCCGGGTGTACTGATCAAATTTGCCTCCTGGTCTTGTAATAGGGTGGCACATAAACTCACTAATCTTGCTTTTGAAACTCTACGAGAAGCATCATGGCAAGGAACAACTCCACCCTGTATTGTTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0031461 GO:0032991 GO:0044424 GO:0044464 GO:0080008 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Katanin_con80 WD40

Protein Analysis

190

Amino Acids

20.68

Weight (kDa)

6.82

Isoelectric Point (pI)

45.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 47 - 170 9.2e-19 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 437
AccB1I GGYRCC 1 cut(s) 437
AcsI RAATTY 2 cut(s) 62, 461
AcuI CTGAAG 1 cut(s) 78
AfaI GTAC 3 cut(s) 379, 439, 453
AgsI TTSAA 6 cut(s) 218, 341, 365, 388, 425, 518
AjnI CCWGG 1 cut(s) 470
AluBI AGCT 3 cut(s) 201, 221, 405
AluI AGCT 3 cut(s) 201, 221, 405
AlwNI CAGNNNCTG 1 cut(s) 380
AoxI GGCC 2 cut(s) 53, 295
ApeKI GCWGC 4 cut(s) 125, 128, 221, 405
ApoI RAATTY 2 cut(s) 62, 461
Asp700I GAANNNNTTC 2 cut(s) 63, 82
Asp718I GGTACC 1 cut(s) 437
AsuC2I CCSGG 1 cut(s) 447
BanI GGYRCC 1 cut(s) 437
BbvI GCAGC 4 cut(s) 112, 115, 208, 392
BccI CCATC 2 cut(s) 111, 140
BciT130I CCWGG 1 cut(s) 472
BclI TGATCA 2 cut(s) 42, 456
BcnI CCSGG 1 cut(s) 447
BfaI CTAG 2 cut(s) 75, 353
BfmI CTRYAG 2 cut(s) 299, 317
BisI GCNGC 4 cut(s) 126, 129, 222, 406
BlsI GCNGC 4 cut(s) 127, 130, 223, 407
Bme1390I CCNGG 2 cut(s) 447, 472
BmiI GGNNCC 3 cut(s) 151, 211, 439
BmrFI CCNGG 2 cut(s) 447, 472
BmsI GCATC 1 cut(s) 542
BpmI CTGGAG 1 cut(s) 204
BpuEI CTTGAG 1 cut(s) 187
BpuMI CCSGG 1 cut(s) 447
Bse118I RCCGGY 1 cut(s) 241
Bse1I ACTGG 2 cut(s) 95, 187
BseBI CCWGG 1 cut(s) 472
BseMII CTCAG 1 cut(s) 408
BseNI ACTGG 2 cut(s) 95, 187
BseRI GAGGAG 1 cut(s) 201
BseXI GCAGC 4 cut(s) 112, 115, 208, 392
BsgI GTGCAG 1 cut(s) 38
BshFI GGCC 2 cut(s) 55, 297
BshNI GGYRCC 1 cut(s) 437
BsiSI CCGG 2 cut(s) 242, 446
BsnI GGCC 2 cut(s) 55, 297
Bsp143I GATC 3 cut(s) 42, 369, 456
BspANI GGCC 2 cut(s) 55, 297
BspCNI CTCAG 1 cut(s) 407
BspLI GGNNCC 3 cut(s) 151, 211, 439
BspMAI CTGCAG 2 cut(s) 303, 321
BspT107I GGYRCC 1 cut(s) 437
BsrFI RCCGGY 1 cut(s) 241
BsrI ACTGG 2 cut(s) 95, 187
BssAI RCCGGY 1 cut(s) 241
BssMI GATC 3 cut(s) 42, 369, 456
Bst2UI CCWGG 1 cut(s) 472
Bst4CI ACNGT 1 cut(s) 279
BstC8I GCNNGC 2 cut(s) 123, 299
BstDEI CTNAG 1 cut(s) 394
BstKTI GATC 3 cut(s) 45, 372, 459
BstMBI GATC 3 cut(s) 42, 369, 456
BstMWI GCNNNNNNNGC 1 cut(s) 218
BstNI CCWGG 1 cut(s) 472
BstSCI CCNGG 2 cut(s) 445, 470
BstSFI CTRYAG 2 cut(s) 299, 317
BstV1I GCAGC 4 cut(s) 112, 115, 208, 392
BsuRI GGCC 2 cut(s) 55, 297
Cac8I GCNNGC 2 cut(s) 123, 299
CaiI CAGNNNCTG 1 cut(s) 380
Cfr10I RCCGGY 1 cut(s) 241
Csp6I GTAC 3 cut(s) 378, 438, 452
CviAII CATG 4 cut(s) 51, 416, 442, 537
CviQI GTAC 3 cut(s) 378, 438, 452
DdeI CTNAG 1 cut(s) 394
DpnI GATC 3 cut(s) 44, 371, 458
DpnII GATC 3 cut(s) 42, 369, 456
Eco147I AGGCCT 1 cut(s) 297
Eco57I CTGAAG 1 cut(s) 78
EcoRII CCWGG 1 cut(s) 470
FaeI CATG 4 cut(s) 54, 419, 445, 540
FatI CATG 4 cut(s) 50, 415, 441, 536
FauNDI CATATG 1 cut(s) 172
FbaI TGATCA 2 cut(s) 42, 456
Fnu4HI GCNGC 4 cut(s) 126, 129, 222, 406
Fsp4HI GCNGC 4 cut(s) 126, 129, 222, 406
FspBI CTAG 2 cut(s) 75, 353
GluI GCNGC 4 cut(s) 126, 129, 222, 406
GsuI CTGGAG 1 cut(s) 204
HaeIII GGCC 2 cut(s) 55, 297
HapII CCGG 2 cut(s) 242, 446
Hin1II CATG 4 cut(s) 54, 419, 445, 540
HinfI GANTC 3 cut(s) 167, 313, 388
HpaII CCGG 2 cut(s) 242, 446
Hpy166II GTNNAC 2 cut(s) 256, 452
Hpy188III TCNNGA 2 cut(s) 310, 365
Hpy8I GTNNAC 2 cut(s) 256, 452
HpyAV CCTTC 1 cut(s) 287
HpyCH4III ACNGT 1 cut(s) 279
HpyCH4V TGCA 5 cut(s) 19, 131, 301, 319, 408
HpyF10VI GCNNNNNNNGC 1 cut(s) 218
HpyF3I CTNAG 1 cut(s) 394
Hsp92II CATG 4 cut(s) 54, 419, 445, 540
KpnI GGTACC 1 cut(s) 441
Ksp22I TGATCA 2 cut(s) 42, 456
Kzo9I GATC 3 cut(s) 42, 369, 456
LmnI GCTCC 2 cut(s) 149, 209
Lsp1109I GCAGC 4 cut(s) 112, 115, 208, 392
LweI GCATC 1 cut(s) 542
MaeI CTAG 2 cut(s) 75, 353
MaeIII GTNAC 1 cut(s) 397
MalI GATC 3 cut(s) 44, 371, 458
MboI GATC 3 cut(s) 42, 369, 456
MboII GAAGA 1 cut(s) 71
MluCI AATT 3 cut(s) 62, 132, 461
MnlI CCTC 2 cut(s) 179, 478
MroXI GAANNNNTTC 2 cut(s) 63, 82
MseI TTAA 1 cut(s) 194
MspI CCGG 2 cut(s) 242, 446
MspR9I CCNGG 2 cut(s) 447, 472
MvaI CCWGG 1 cut(s) 472
MwoI GCNNNNNNNGC 1 cut(s) 218
NciI CCSGG 1 cut(s) 447
NdeI CATATG 1 cut(s) 172
NdeII GATC 3 cut(s) 42, 369, 456
NlaIII CATG 4 cut(s) 54, 419, 445, 540
NlaIV GGNNCC 3 cut(s) 151, 211, 439
NmeAIII GCCGAG 1 cut(s) 239
PceI AGGCCT 1 cut(s) 297
PdmI GAANNNNTTC 2 cut(s) 63, 82
PfeI GAWTC 3 cut(s) 167, 313, 388
PkrI GCNGC 4 cut(s) 127, 130, 223, 407
Psp6I CCWGG 1 cut(s) 470
PspGI CCWGG 1 cut(s) 470
PspN4I GGNNCC 3 cut(s) 151, 211, 439
PstI CTGCAG 2 cut(s) 303, 321
PstNI CAGNNNCTG 1 cut(s) 380
RsaI GTAC 3 cut(s) 379, 439, 453
RsaNI GTAC 3 cut(s) 378, 438, 452
SaqAI TTAA 1 cut(s) 194
SatI GCNGC 4 cut(s) 126, 129, 222, 406
Sau3AI GATC 3 cut(s) 42, 369, 456
ScrFI CCNGG 2 cut(s) 447, 472
SetI ASST 5 cut(s) 203, 223, 273, 379, 407
SfaNI GCATC 1 cut(s) 542
SfcI CTRYAG 2 cut(s) 299, 317
SmlI CTYRAG 1 cut(s) 202
SmoI CTYRAG 1 cut(s) 202
Sse9I AATT 3 cut(s) 62, 132, 461
SseBI AGGCCT 1 cut(s) 297
SspMI CTAG 2 cut(s) 75, 353
StuI AGGCCT 1 cut(s) 297
StyD4I CCNGG 2 cut(s) 445, 470
TaaI ACNGT 1 cut(s) 279
TasI AATT 3 cut(s) 62, 132, 461
TatI WGTACW 1 cut(s) 451
TfiI GAWTC 3 cut(s) 167, 313, 388
Tru1I TTAA 1 cut(s) 194
Tru9I TTAA 1 cut(s) 194
TseI GCWGC 4 cut(s) 125, 128, 221, 405
TspDTI ATGAA 1 cut(s) 295
XapI RAATTY 2 cut(s) 62, 461
XcmI CCANNNNNNNNNTGG 1 cut(s) 170
XmnI GAANNNNTTC 2 cut(s) 63, 82
XspI CTAG 2 cut(s) 75, 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.