MD16G1005500.v1.1

May participate in a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0005757
Physical Location & Seq
Forward (+)
425958 .. 435003
9046 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1005500.v1.1.491

Sequence Viewer

Length: 2346 bp
ATGACTACTAAACGCGCTTACAAGCTACAGGAATTTGTAGCACATTCTGCAGCTGTGAATTGCCTCAAGATTGGCAGAAAATCTTCTAGAGTTCTTGTCACCGGCGGAGAAGATCACAAGGTCAATCTCTGGGCCATTGGGAAACCCAATGCCTTACTGAGTTTGTCTGGACACACCAGTGGAATTGATTCAGTAAGCTTTGATTCTTCAGAGGTCTTAGTAGCTGCAGGAGCTGCAAGTGGTACAATTAAGCTTTGGGATTTAGAGGAGGCAAAGATTGTTCGCACGCTTACCGGTCACAGATCCAATTGTATATCTGTGGACTTTCATCCTTTTGGAGAGTTCTTTGCATCTGGCTCCTTAGATACAAATCTCAAAATATGGGATATCAGGAAGAAGGGGTGTATTCACACATACAAAGGCCATACTCGAGGGGTCAATGCTATCAGATTTACCCCAGATGGCCGCTGGGTTGTTTCTGGTGGAGAGGACAACACTGTGAAGTTGTGGGACCTGACCGCTGGAAAGCTGCTGCATGACTTCAAGTGTCATGAGGGCCAGGTTCAGTGCATAGACTTTCATCCCCACGAGTTTCTGCTAGCAACAGGTTCAGCGGATAGGACTGTCAAATTTTGGGACCTTGAAACCTTCGAGCTAATTGGCTCTGCTGGACCTGAGACAACTGGAGTTTTCTCGTGGGAGCCAATAAGGTGCCATGATGCTGTGGAGGTGGGATGGTCTCGATTGTCAGATCTTAATGTTCATGAGGGAAAGCTTCTTGGATGCTCTTACAATCAGAGTTGTGTTGGAGTGTGGGTTGTAGACATCTCGCGCATTGAACCGTATGCACTTGGTAATGCCAGCAGATTGAGTGGGCATTCAGAATCTAAATCATTGTCTGGTGGGAATCTCTCTGTGCTCAATGAGAACGCTGCTAAATCTAGTTTGGGCCGGTCATCTGTTTCTCAAAATTCAGATTCTTTAGTGAAGGAAACAAAATCTCTTGGAAGGTTATCAGTTTCTCAGAATTCAGATCTCGTTAAGGAGCCAAAAATTATTGCATCCACAGGAATTGTTCCTGGTACTCCTCAAAGGATCAATATGAATTCCAGTCAAAAAACTATTGTGGCTAGTTCAGTGGCAGTTCCTAATGCATCAACCCTGAAGAGGAGTTCTACTAAGACCCATTCAACAGCTAATATTCCAACTTTTAACAAGTCTGATGTCGTACCTGTAATTGTGCCAAGAACTAGTATGAGGTCAGAGCTGTCAGCTGAGTCTAGAAAAGAGGTTAGCGTTGCTGGAAGACCACTGGCATTTTCTTTGCAGTCAAAGGCAACTGATTTCCGGAAGTTGTCCAATAGCAGAGAAGAAGTGGATAAGCCAGCTGTCTCTTCAGTGTCTGAACACCCTGCTTCTAAGGCTATTGAATTAAGCAGTGCTGCAGATAAGAATGGCTTCTCAACAGTTTTAAGTTCAACTCAGGGAATACATGCTGAAAAGAACACAAAGGATGATATATATTTTGGGCCTGGGAAGCATGAAACTAGTTCAATGATGGAGCCAACTGCAAGTTACCAGCATGAAAGTTATGAGGCTCCAGGGCACAAGGTGAGCAGAGAAGCATTTTCTGCTGAAGGTCAAAGAGGAGGGAGATTGCGCTCAATTGCTGTAAATTGGGAGAAAAGAGATAGATATTCTAACTACGAGGGACCTACTTCCAATGGTACCCTGGGAACAGCTTCTGCACCAAATATTATCCCTTTAAATGCGTTTAAGCAGCGAGGTTATCCTACATCTACTGAAAAAGAAACAGTGTCTGCTAGTGATGAGGATGCTATTGCAGATCTCCTGGAAAAACACGATCAGTTTGTTGGCTCCATGCAATCTCGTGTTACCAAATTACAGTTAGTCTATAGATATTGGGAAAGGAATGATGTTAAGGGGGTCCTTGGTGCAATGGAGAAGATGGCTGATCATGCTGTCATTGCGGATGTAGTCAGCATTATGACAGAAAAATTTGATATTGTCACACTGGATATATGCACTTGCCTTCTGCCACTCCTCACTGGCCTTCTAGAAAGTAACATGGATAGGCATTTAGGCATTTCTTTGGAAATGCTGCTCAAGCTGGTTAGAGTGTTTGGTTCTGTGATCTATTCAGCAATATCTGCCTCGTCATCTGTTGGCGTTGATATTGAAGCCGAGAAAAGGCTGGAGCGTTGCAACGTCTGTTTTGTGGAGCTTGAAAAGGTCAAATGCTGTTTGCCGGCTCTAACCAGAAGAGGAGGATCAATTGCAAAGGCTGCACAGGAGTTGAATTTAGCTCTTCAGGAAGTTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0031461 GO:0032991 GO:0044424 GO:0044464 GO:0080008 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Katanin_con80 WD40

Protein Analysis

782

Amino Acids

84.85

Weight (kDa)

7.6

Isoelectric Point (pI)

32.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR36-Utp21_1st PF25171 6 - 128 7.8e-08 WDR36/Utp21 first beta-propeller
WD40_MABP1-WDR62_2nd PF24782 6 - 172 1.3e-12 MABP1/WDR62 second WD40 domain
Beta-prop_THOC3 PF25174 7 - 89 6.1e-09 THOC3 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 9 - 130 4.8e-11 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 10 - 138 8.1e-26 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 10 - 130 4.9e-23 WDR3 first beta-propeller domain
WD40_Gbeta PF25391 11 - 132 7.9e-14 G protein beta WD-40 repeat protein
Beta-prop_WDR36-Utp21_2nd PF25168 13 - 133 1.1e-12 WDR36/Utp21 second beta-propeller domain
Beta-prop_SCAP PF24017 15 - 129 1.1e-09 SCAP Beta-propeller
WD40_Prp19 PF24814 15 - 216 2.6e-44 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 16 - 97 1.8e-10 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 28 - 133 7.8e-11 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 28 - 128 5.3e-07 WDR90/POC16, second beta-propeller
WD40_CDC20-Fz PF24807 50 - 221 2.5e-32 CDC20/Fizzy WD40 domain
WD40 PF00400 52 - 87 2.9e-06 WD domain, G-beta repeat
Beta-prop_WDR3_2nd PF25172 52 - 174 8.5e-18 WDR3 second beta-propeller domain
EIF3I PF24805 53 - 139 8.9e-06 EIF3I
Beta-prop_CAF1B_HIR1 PF24105 54 - 138 2.5e-06 CAF1B/HIR1 beta-propeller domain
Beta-prop_THOC3 PF25174 58 - 201 1.7e-34 THOC3 beta-propeller domain
WDR55 PF24796 62 - 223 4.5e-18 WDR55
Beta-prop_EIPR1 PF23609 67 - 170 1e-06 EIPR1 beta-propeller
WD40_WDHD1_1st PF24817 73 - 141 4.3e-12 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 74 - 239 4.9e-18 Echinoderm microtubule-associated protein second beta-propeller
WD40 PF00400 92 - 129 3e-09 WD domain, G-beta repeat
Beta-prop_TEP1_2nd PF25047 95 - 181 8.1e-07 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 96 - 218 5.1e-34 WDR5 beta-propeller domain
Beta-prop_EML PF23409 98 - 217 2.6e-10 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_Aladin PF25460 102 - 205 2.4e-07 Aladin seven-bladed propeller
Beta-prop_WDR3_1st PF25173 115 - 220 1.2e-26 WDR3 first beta-propeller domain
Beta-prop_SCAP PF24017 118 - 193 3.2e-06 SCAP Beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 119 - 221 2.1e-11 WDR36/Utp21 first beta-propeller
WD40_Gbeta PF25391 121 - 216 1e-13 G protein beta WD-40 repeat protein
Beta-prop_WDR36-Utp21_2nd PF25168 129 - 206 2.3e-11 WDR36/Utp21 second beta-propeller domain
WD40 PF00400 135 - 171 1.1e-13 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 137 - 218 1.8e-11 CAF1B/HIR1 beta-propeller domain
WD40_MABP1-WDR62_1st PF24780 137 - 215 2.1e-06 MABP1/WDR62 first WD40 domain
Beta-prop_TEP1_2nd PF25047 137 - 217 1.1e-07 TEP-1 second beta-propeller
EIF3I PF24805 140 - 221 3.5e-13 EIF3I
ANAPC4_WD40 PF12894 142 - 194 2.3e-06 Anaphase-promoting complex subunit 4 WD40 domain
WD40_WDHD1_1st PF24817 144 - 216 3.3e-11 WDHD1 first WD40 domain
WD40_MABP1-WDR62_2nd PF24782 144 - 215 5.1e-07 MABP1/WDR62 second WD40 domain
Beta-prop_WDR90_POC16_2nd PF23393 163 - 220 2.5e-07 WDR90/POC16, second beta-propeller
Beta-prop_THOC3 PF25174 166 - 219 1.3e-10 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 167 - 218 7.9e-07 WDR3 second beta-propeller domain
WD40 PF00400 175 - 213 5.4e-10 WD domain, G-beta repeat
Katanin_con80 PF13925 620 - 777 1.7e-54 con80 domain of Katanin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1727
AccB1I GGYRCC 2 cut(s) 711, 1727
AccI GTMKAC 1 cut(s) 822
AccII CGCG 2 cut(s) 15, 832
AccIII TCCGGA 1 cut(s) 1347
AciI CCGC 5 cut(s) 105, 466, 519, 614, 1991
AclWI GGATC 3 cut(s) 297, 1103, 2299
AcoI YGGCCR 1 cut(s) 463
AcsI RAATTY 7 cut(s) 32, 629, 970, 1027, 1105, 2018, 2320
AcuI CTGAAG 5 cut(s) 192, 1184, 1380, 1656, 2315
AdeI CACNNNGTG 1 cut(s) 1612
AfaI GTAC 4 cut(s) 244, 1084, 1230, 1729
AfiI CCNNNNNNNGG 2 cut(s) 1167, 2211
AgeI ACCGGT 1 cut(s) 293
AhlI ACTAGT 2 cut(s) 1250, 1547
AjnI CCWGG 6 cut(s) 558, 1078, 1531, 1600, 1731, 1851
AleI CACNNNNGTG 1 cut(s) 177
Alw21I GWGCWC 1 cut(s) 921
Alw26I GTCTC 3 cut(s) 671, 744, 1396
AlwI GGATC 3 cut(s) 297, 1103, 2299
AlwNI CAGNNNCTG 4 cut(s) 233, 1403, 1745, 1820
Ama87I CYCGRG 1 cut(s) 429
Aor13HI TCCGGA 1 cut(s) 1347
AoxI GGCC 7 cut(s) 132, 421, 463, 556, 949, 1529, 2071
ApoI RAATTY 7 cut(s) 32, 629, 970, 1027, 1105, 2018, 2320
AsiGI ACCGGT 1 cut(s) 293
Asp700I GAANNNNTTC 4 cut(s) 82, 187, 1457, 1741
Asp718I GGTACC 1 cut(s) 1727
AspLEI GCGC 3 cut(s) 17, 834, 1662
AspS9I GGNCC 9 cut(s) 132, 511, 556, 637, 671, 949, 1529, 1712, 1948
AsuHPI GGTGA 2 cut(s) 91, 1624
AsuNHI GCTAGC 1 cut(s) 598
AvaI CYCGRG 1 cut(s) 429
AvaII GGWCC 5 cut(s) 511, 637, 671, 1712, 1948
BaeGI GKGCMC 1 cut(s) 1608
BanI GGYRCC 2 cut(s) 711, 1727
BauI CACGAG 3 cut(s) 587, 694, 1890
BbsI GAAGAC 1 cut(s) 1312
Bbv12I GWGCWC 1 cut(s) 921
BccI CCATC 4 cut(s) 455, 729, 1552, 1963
BciT130I CCWGG 6 cut(s) 560, 1080, 1533, 1602, 1733, 1853
BclI TGATCA 1 cut(s) 1975
BcoDI GTCTC 3 cut(s) 671, 744, 1396
BcuI ACTAGT 2 cut(s) 1250, 1547
BfaI CTAG 9 cut(s) 87, 599, 942, 1131, 1251, 1281, 1548, 1824, 2078
BfmI CTRYAG 5 cut(s) 26, 48, 225, 1443, 1915
BglII AGATCT 3 cut(s) 751, 1033, 1846
Bme1390I CCNGG 6 cut(s) 560, 1080, 1533, 1602, 1733, 1853
Bme18I GGWCC 5 cut(s) 511, 637, 671, 1712, 1948
BmeT110I CYCGRG 1 cut(s) 429
BmgT120I GGNCC 9 cut(s) 132, 511, 556, 637, 671, 949, 1529, 1712, 1948
BmrFI CCNGG 6 cut(s) 560, 1080, 1533, 1602, 1733, 1853
BmsI GCATC 6 cut(s) 359, 709, 773, 1070, 1163, 1825
BmtI GCTAGC 1 cut(s) 602
BpiI GAAGAC 1 cut(s) 1312
BpmI CTGGAG 3 cut(s) 705, 1584, 2237
BpuEI CTTGAG 2 cut(s) 50, 2111
BsaBI GATNNNNATC 1 cut(s) 369
BsaI GGTCTC 1 cut(s) 744
BsaJI CCNNGG 5 cut(s) 1532, 1601, 1731, 1732, 1951
BsaWI WCCGGW 2 cut(s) 293, 1347
BsaXI ACNNNNNCTCC 2 cut(s) 801, 831
Bsc4I CCNNNNNNNGG 2 cut(s) 1167, 2211
Bse118I RCCGGY 4 cut(s) 101, 293, 951, 2269
Bse1I ACTGG 6 cut(s) 177, 688, 1110, 1317, 2040, 2074
Bse3DI GCAATG 2 cut(s) 1965, 1986
Bse8I GATNNNNATC 1 cut(s) 369
BseAI TCCGGA 1 cut(s) 1347
BseBI CCWGG 6 cut(s) 560, 1080, 1533, 1602, 1733, 1853
BseDI CCNNGG 5 cut(s) 1532, 1601, 1731, 1732, 1951
BseGI GGATG 8 cut(s) 328, 580, 740, 788, 1061, 1519, 1840, 1999
BseJI GATNNNNATC 1 cut(s) 369
BseLI CCNNNNNNNGG 2 cut(s) 1167, 2211
BseMI GCAATG 2 cut(s) 1965, 1986
BseMII CTCAG 5 cut(s) 149, 666, 1037, 1266, 1496
BseNI ACTGG 6 cut(s) 177, 688, 1110, 1317, 2040, 2074
BseRI GAGGAG 6 cut(s) 281, 1077, 1183, 1662, 2054, 2301
BseSI GKGCMC 1 cut(s) 1608
BseYI CCCAGC 1 cut(s) 468
BsgI GTGCAG 2 cut(s) 1731, 2292
Bsh1236I CGCG 2 cut(s) 15, 832
BshFI GGCC 7 cut(s) 134, 423, 465, 558, 951, 1531, 2073
BshNI GGYRCC 2 cut(s) 711, 1727
BshTI ACCGGT 1 cut(s) 293
BsiHKAI GWGCWC 1 cut(s) 921
BsiHKCI CYCGRG 1 cut(s) 429
BsiSI CCGG 5 cut(s) 102, 294, 952, 1348, 2270
BslFI GGGAC 3 cut(s) 524, 650, 1725
BslI CCNNNNNNNGG 2 cut(s) 1167, 2211
BsmAI GTCTC 3 cut(s) 671, 744, 1396
BsmFI GGGAC 3 cut(s) 524, 650, 1725
BsmI GAATGC 1 cut(s) 877
BsnI GGCC 7 cut(s) 134, 423, 465, 558, 951, 1531, 2073
Bso31I GGTCTC 1 cut(s) 744
BsoBI CYCGRG 1 cut(s) 429
Bsp1286I GDGCHC 2 cut(s) 921, 1608
Bsp13I TCCGGA 1 cut(s) 1347
BspACI CCGC 5 cut(s) 105, 466, 519, 614, 1991
BspANI GGCC 7 cut(s) 134, 423, 465, 558, 951, 1531, 2073
BspCNI CTCAG 5 cut(s) 150, 667, 1036, 1267, 1495
BspEI TCCGGA 1 cut(s) 1347
BspFNI CGCG 2 cut(s) 15, 832
BspHI TCATGA 3 cut(s) 550, 763, 2342
BspMAI CTGCAG 3 cut(s) 52, 229, 1447
BspOI GCTAGC 1 cut(s) 602
BspPI GGATC 3 cut(s) 297, 1103, 2299
BspQI GCTCTTC 1 cut(s) 2334
BspT107I GGYRCC 2 cut(s) 711, 1727
BspTNI GGTCTC 1 cut(s) 744
BsrDI GCAATG 2 cut(s) 1965, 1986
BsrFI RCCGGY 4 cut(s) 101, 293, 951, 2269
BsrI ACTGG 6 cut(s) 177, 688, 1110, 1317, 2040, 2074
BssAI RCCGGY 4 cut(s) 101, 293, 951, 2269
BssECI CCNNGG 5 cut(s) 1532, 1601, 1731, 1732, 1951
BssSI CACGAG 3 cut(s) 587, 694, 1890
BssT1I CCWWGG 1 cut(s) 1951
Bst2BI CACGAG 3 cut(s) 587, 694, 1890
Bst2UI CCWGG 6 cut(s) 560, 1080, 1533, 1602, 1733, 1853
Bst4CI ACNGT 6 cut(s) 499, 625, 843, 1468, 1816, 1908
Bst6I CTCTTC 4 cut(s) 1160, 1399, 2278, 2334
BstAPI GCANNNNNTGC 5 cut(s) 47, 233, 1631, 2171, 2306
BstC8I GCNNGC 5 cut(s) 287, 600, 862, 1386, 2271
BstDEI CTNAG 9 cut(s) 158, 217, 361, 675, 1023, 1179, 1275, 1419, 1482
BstF5I GGATG 8 cut(s) 328, 580, 740, 788, 1061, 1519, 1840, 1999
BstFNI CGCG 2 cut(s) 15, 832
BstHHI GCGC 3 cut(s) 17, 834, 1662
BstMAI GTCTC 3 cut(s) 671, 744, 1396
BstNI CCWGG 6 cut(s) 560, 1080, 1533, 1602, 1733, 1853
BstNSI RCATGY 1 cut(s) 1496
BstSCI CCNGG 6 cut(s) 558, 1078, 1531, 1600, 1731, 1851
BstSFI CTRYAG 5 cut(s) 26, 48, 225, 1443, 1915
BstSLI GKGCMC 1 cut(s) 1608
BstUI CGCG 2 cut(s) 15, 832
BstV2I GAAGAC 1 cut(s) 1312
BstX2I RGATCY 4 cut(s) 302, 751, 1033, 1846
BstYI RGATCY 4 cut(s) 302, 751, 1033, 1846
BsuRI GGCC 7 cut(s) 134, 423, 465, 558, 951, 1531, 2073
BtsCI GGATG 8 cut(s) 328, 580, 740, 788, 1061, 1519, 1840, 1999
BtsI GCAGTG 1 cut(s) 1444
Cac8I GCNNGC 5 cut(s) 287, 600, 862, 1386, 2271
CaiI CAGNNNCTG 4 cut(s) 233, 1403, 1745, 1820
CciI TCATGA 3 cut(s) 550, 763, 2342
CfoI GCGC 3 cut(s) 17, 834, 1662
Cfr10I RCCGGY 4 cut(s) 101, 293, 951, 2269
Cfr13I GGNCC 9 cut(s) 132, 511, 556, 637, 671, 949, 1529, 1712, 1948
Csp6I GTAC 4 cut(s) 243, 1083, 1229, 1728
CspAI ACCGGT 1 cut(s) 293
CspCI CAANNNNNGTGG 2 cut(s) 1054, 1089
CviQI GTAC 4 cut(s) 243, 1083, 1229, 1728
DdeI CTNAG 9 cut(s) 158, 217, 361, 675, 1023, 1179, 1275, 1419, 1482
DraI TTTAAA 1 cut(s) 1767
DraIII CACNNNGTG 1 cut(s) 1612
EaeI YGGCCR 1 cut(s) 463
Eam1104I CTCTTC 4 cut(s) 1160, 1399, 2278, 2334
EarI CTCTTC 4 cut(s) 1160, 1399, 2278, 2334
EciI GGCGGA 1 cut(s) 120
Eco130I CCWWGG 1 cut(s) 1951
Eco31I GGTCTC 1 cut(s) 744
Eco32I GATATC 1 cut(s) 388
Eco47I GGWCC 5 cut(s) 511, 637, 671, 1712, 1948
Eco57I CTGAAG 5 cut(s) 192, 1184, 1380, 1656, 2315
Eco88I CYCGRG 1 cut(s) 429
EcoO109I RGGNCCY 4 cut(s) 511, 637, 1712, 1948
EcoRI GAATTC 2 cut(s) 1027, 1105
EcoRII CCWGG 6 cut(s) 558, 1078, 1531, 1600, 1731, 1851
EcoRV GATATC 1 cut(s) 388
EcoT14I CCWWGG 1 cut(s) 1951
EcoT22I ATGCAT 1 cut(s) 1156
ErhI CCWWGG 1 cut(s) 1951
FalI AAGNNNNNCTT 2 cut(s) 1442, 1474
FaqI GGGAC 3 cut(s) 524, 650, 1725
FbaI TGATCA 1 cut(s) 1975
FblI GTMKAC 1 cut(s) 822
FokI GGATG 8 cut(s) 315, 567, 747, 795, 1048, 1526, 1847, 2006
FspBI CTAG 9 cut(s) 87, 599, 942, 1131, 1251, 1281, 1548, 1824, 2078
GlaI GCGC 3 cut(s) 16, 833, 1661
GsaI CCCAGC 1 cut(s) 472
GsuI CTGGAG 3 cut(s) 705, 1584, 2237
HaeIII GGCC 7 cut(s) 134, 423, 465, 558, 951, 1531, 2073
HapII CCGG 5 cut(s) 102, 294, 952, 1348, 2270
HhaI GCGC 3 cut(s) 17, 834, 1662
Hin6I GCGC 3 cut(s) 15, 832, 1660
HinP1I GCGC 3 cut(s) 15, 832, 1660
HindIII AAGCTT 3 cut(s) 196, 251, 773
HinfI GANTC 6 cut(s) 188, 203, 884, 907, 977, 1277
HpaII CCGG 5 cut(s) 102, 294, 952, 1348, 2270
HphI GGTGA 2 cut(s) 91, 1624
Hpy166II GTNNAC 2 cut(s) 322, 823
Hpy8I GTNNAC 2 cut(s) 322, 823
HpyAV CCTTC 7 cut(s) 391, 658, 982, 1002, 1631, 2063, 2084
HpyCH4III ACNGT 6 cut(s) 499, 625, 843, 1468, 1816, 1908
HpyCH4IV ACGT 1 cut(s) 2229
HpyF3I CTNAG 9 cut(s) 158, 217, 361, 675, 1023, 1179, 1275, 1419, 1482
HpySE526I ACGT 1 cut(s) 2229
HspAI GCGC 3 cut(s) 15, 832, 1660
Kpn2I TCCGGA 1 cut(s) 1347
KpnI GGTACC 1 cut(s) 1731
KroI GCCGGC 1 cut(s) 2269
KroNI GCCGGC 1 cut(s) 2271
Ksp22I TGATCA 1 cut(s) 1975
LguI GCTCTTC 1 cut(s) 2334
LmnI GCTCC 9 cut(s) 230, 362, 700, 1045, 1561, 1603, 1883, 2218, 2242
LweI GCATC 6 cut(s) 359, 709, 773, 1070, 1163, 1825
MaeI CTAG 9 cut(s) 87, 599, 942, 1131, 1251, 1281, 1548, 1824, 2078
MaeII ACGT 1 cut(s) 2229
MaeIII GTNAC 6 cut(s) 97, 296, 1574, 1894, 2029, 2084
MfeI CAATTG 3 cut(s) 307, 1665, 2295
MflI RGATCY 4 cut(s) 302, 751, 1033, 1846
MhlI GDGCHC 2 cut(s) 921, 1608
MlyI GAGTC 1 cut(s) 1286
MmeI TCCRAC 2 cut(s) 787, 1229
Mph1103I ATGCAT 1 cut(s) 1156
MroI TCCGGA 1 cut(s) 1347
MroNI GCCGGC 1 cut(s) 2269
MroXI GAANNNNTTC 4 cut(s) 82, 187, 1457, 1741
MseI TTAA 9 cut(s) 249, 756, 1041, 1212, 1433, 1472, 1766, 1776, 1941
MslI CAYNNNNRTG 1 cut(s) 177
MspA1I CMGCKG 6 cut(s) 53, 468, 521, 614, 1274, 1388
MspI CCGG 5 cut(s) 102, 294, 952, 1348, 2270
MspR9I CCNGG 6 cut(s) 560, 1080, 1533, 1602, 1733, 1853
MunI CAATTG 3 cut(s) 307, 1665, 2295
Mva1269I GAATGC 1 cut(s) 877
MvaI CCWGG 6 cut(s) 560, 1080, 1533, 1602, 1733, 1853
MvnI CGCG 2 cut(s) 15, 832
NaeI GCCGGC 1 cut(s) 2271
NgoMIV GCCGGC 1 cut(s) 2269
NheI GCTAGC 1 cut(s) 598
NmeAIII GCCGAG 1 cut(s) 2230
NmuCI GTSAC 3 cut(s) 97, 296, 2029
NsiI ATGCAT 1 cut(s) 1156
NspI RCATGY 1 cut(s) 1496
OliI CACNNNNGTG 1 cut(s) 177
PaeR7I CTCGAG 1 cut(s) 429
PagI TCATGA 3 cut(s) 550, 763, 2342
PasI CCCWGGG 1 cut(s) 1732
PciSI GCTCTTC 1 cut(s) 2334
PctI GAATGC 1 cut(s) 877
PdiI GCCGGC 1 cut(s) 2271
PdmI GAANNNNTTC 4 cut(s) 82, 187, 1457, 1741
PfeI GAWTC 5 cut(s) 188, 203, 884, 907, 977
PfoI TCCNGGA 1 cut(s) 1851
PinAI ACCGGT 1 cut(s) 293
PleI GAGTC 1 cut(s) 1285
PpsI GAGTC 1 cut(s) 1285
PpuMI RGGWCCY 4 cut(s) 511, 637, 1712, 1948
Psp5II RGGWCCY 4 cut(s) 511, 637, 1712, 1948
Psp6I CCWGG 6 cut(s) 558, 1078, 1531, 1600, 1731, 1851
PspFI CCCAGC 1 cut(s) 468
PspGI CCWGG 6 cut(s) 558, 1078, 1531, 1600, 1731, 1851
PspPI GGNCC 9 cut(s) 132, 511, 556, 637, 671, 949, 1529, 1712, 1948
PspPPI RGGWCCY 4 cut(s) 511, 637, 1712, 1948
PspXI VCTCGAGB 1 cut(s) 429
PstI CTGCAG 3 cut(s) 52, 229, 1447
PstNI CAGNNNCTG 4 cut(s) 233, 1403, 1745, 1820
PsuI RGATCY 4 cut(s) 302, 751, 1033, 1846
PvuII CAGCTG 3 cut(s) 53, 1274, 1388
RsaI GTAC 4 cut(s) 244, 1084, 1230, 1729
RsaNI GTAC 4 cut(s) 243, 1083, 1229, 1728
RseI CAYNNNNRTG 1 cut(s) 177
SapI GCTCTTC 1 cut(s) 2334
SaqAI TTAA 9 cut(s) 249, 756, 1041, 1212, 1433, 1472, 1766, 1776, 1941
Sau96I GGNCC 9 cut(s) 132, 511, 556, 637, 671, 949, 1529, 1712, 1948
SchI GAGTC 1 cut(s) 1286
ScrFI CCNGG 6 cut(s) 560, 1080, 1533, 1602, 1733, 1853
SduI GDGCHC 2 cut(s) 921, 1608
SfaNI GCATC 6 cut(s) 359, 709, 773, 1070, 1163, 1825
SfcI CTRYAG 5 cut(s) 26, 48, 225, 1443, 1915
Sfr274I CTCGAG 1 cut(s) 429
SgrAI CRCCGGYG 1 cut(s) 101
SinI GGWCC 5 cut(s) 511, 637, 671, 1712, 1948
SlaI CTCGAG 1 cut(s) 429
SmiMI CAYNNNNRTG 1 cut(s) 177
SmlI CTYRAG 3 cut(s) 65, 429, 2126
SmoI CTYRAG 3 cut(s) 65, 429, 2126
SpeI ACTAGT 2 cut(s) 1250, 1547
SsiI CCGC 5 cut(s) 105, 466, 519, 614, 1991
SspI AATATT 2 cut(s) 1201, 1756
SspMI CTAG 9 cut(s) 87, 599, 942, 1131, 1251, 1281, 1548, 1824, 2078
StyD4I CCNGG 6 cut(s) 558, 1078, 1531, 1600, 1731, 1851
StyI CCWWGG 1 cut(s) 1951
TaaI ACNGT 6 cut(s) 499, 625, 843, 1468, 1816, 1908
TaiI ACGT 1 cut(s) 2232
TaqI TCGA 3 cut(s) 430, 651, 742
TauI GCSGC 1 cut(s) 468
TfiI GAWTC 5 cut(s) 188, 203, 884, 907, 977
Tru1I TTAA 9 cut(s) 249, 756, 1041, 1212, 1433, 1472, 1766, 1776, 1941
Tru9I TTAA 9 cut(s) 249, 756, 1041, 1212, 1433, 1472, 1766, 1776, 1941
TseFI GTSAC 3 cut(s) 97, 296, 2029
Tsp45I GTSAC 3 cut(s) 97, 296, 2029
TspDTI ATGAA 7 cut(s) 317, 569, 752, 1118, 1557, 1599, 2331
VpaK11BI GGWCC 5 cut(s) 511, 637, 671, 1712, 1948
XapI RAATTY 7 cut(s) 32, 629, 970, 1027, 1105, 2018, 2320
XbaI TCTAGA 3 cut(s) 86, 1280, 2077
XceI RCATGY 1 cut(s) 1496
XcmI CCANNNNNNNNNTGG 2 cut(s) 465, 1729
XhoI CTCGAG 1 cut(s) 429
XmiI GTMKAC 1 cut(s) 822
XmnI GAANNNNTTC 4 cut(s) 82, 187, 1457, 1741
XspI CTAG 9 cut(s) 87, 599, 942, 1131, 1251, 1281, 1548, 1824, 2078
Zsp2I ATGCAT 1 cut(s) 1156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.