AT5G23430

May participate in a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005757
Physical Location & Seq
Reverse (-)
7893594 .. 7900563
6970 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G23430.3

Sequence Viewer

Length: 2514 bp
ATGACCACCAAGCGAGCCTATAAGCTTCAGGAATTTGTGGCACATTCTGCTGCTGTCAATTGTCTTAAGATCGGAAGGAAGTCTTCTAGGGTTCTGGTAACAGGTGGTGAAGATCACAAGGTCAATCTTTGGGCTATTGGTAAGCCCAATGCCATTCTGAGCTTGTATGGGCACTCAAGTGGAATTGATTCAGTGACGTTTGATGCTTCGGAGGTCTTAGTTGCAGCAGGAGCTGCTAGTGGTACTATCAAACTTTGGGATTTAGAGGAGGCAAAGATTGTCAGGACTCTCACTGGTCACAGGTCCAATTGCATCTCAGTGGATTTCCACCCTTTTGGTGAGTTTTTTGCGTCAGGCTCACTAGACACAAATCTTAAGATATGGGATATACGAAAAAAGGGCTGCATCCATACTTACAAGGGTCACACGCGAGGAGTCAATGTACTCAGATTCACCCCAGATGGTCGCTGGGTTGTATCTGGAGGAGAGGACAACATTGTTAAGGTTTGGGATTTAACAGCTGGAAAACTGCTGACCGAGTTTAAGAGTCATGAAGGGCAAATTCAAAGCCTAGATTTTCATCCTCATGAATTCCTACTGGCAACAGGTTCAGCTGATAGAACTGTAAAATTCTGGGATCTGGAAACATTCGAGCTGATTGGTTCTGGTGGACCTGAGACTGCCGGGGTCCGTTGCCTGAGTTTCAATCCAGATGGAAAGACTGTGCTTTGTGGATTGCAAGAAAGTCTCAAGATTTTCTCATGGGAGCCAATTAGATGTCATGATGGAGTGGATGTTGGATGGTCAAGATTGTCAGATATGAATGTTCACGAGGGAAAGCTTCTTGGTTGCTCATACAATCAGAGTTGTGTAGGCGTGTGGGTTGTAGATCTCTCGCGTACTGAGCCATGTATGGCTGGAGACACTGCGCAATCAAATGGTCATCCAGAGAAACGATCTTGCTCAGGAAGAGATCCAGTAGTTCTCAATGATAATAACTCAAAGACAGTTCTCGGGAAGCTGTCCGTTTCCCAAAATGTAGATCCTTTATTGAAGGAAACAAAGTCTCTAGGAAGATTATCTGTTTCTCAGAACTCTGATCCTTCGACAAAAGAAACAAAGTCCATTGGAAGGTCATCAACATCACAAAATTCAGAGTCCTCGATGAAGGAATCAAAGCCTCTCGGGAGGTTGTCAGTTTCTCAAAATTCAGACGTTTCTAAAGAGTCAAGGACGTTTTCATCCACAGGAAGTTTACCAGGCACTCCTCATAGGGTCAGTTCGACCAATGTATCAAAAGCCACTTCGGGTGTTTCAACAGCCGTCTCTAATGCTGCAACGTCAAGGAGGAATTTTACAAAAGCTAACCCAAAGGCGAATCCTGTCAATAAGGCAGCAGATTTTGCTCCGGTGATTGTCCCCAGAGCAGACCCTAGAATAGAGCAGGCTACTGAATCCAGAGCAGAACTTGACATAATTGCAAGAACCATGCCGTATTCATTGCAGGCGGCTGATTCCCGAAGGTCACCTAGCAGCAGAAATAACCCTGATCTGCCAGACGCTTCGGTTCTTGAAATGTCTGAGTCTCAGCCAGTTGAACCAAATAATATACCAGATGGGGGTACACTTCCTGGTGGAAAGGTTGGCATGCGGGGTGCAACTGAGCGAAGCATTAATGATTTTAGATACAAGAGATATGGCAGGAGTAATTCAAGGTCACGGATGGGGTCACCTCCACGAAACCACGATGAAAACTATGACTTGGTAAGCCACAGATCAAATAGAGACCCAAGCCCCACTGAAAGTCAGAAAGGAGGAAGATTCCAGTCACTCGTCATAAATAGGGAGAGGAGAGGAAGATTTTCAAACTTCGAGGGTCCTGTTTCAAATTTTTCAAGTGGAAATATGCCTGCTCCAAACATCCGCCCTTCAAATATGTTCAAGCAGAGAGGGAACCATATGCCAGTTGAACAAGGGATCGATTCTCCTTCTGAAGAAAATATTGTTGAAGACATAATGGGAAAGCACAATCAATTTGTCAGCTCTATGCAGTCTCGTTTGGCTAAGTTACAGGTAGTTCGTAGATATTGGGAAAGGAATGATGTAAAAAATTCGATAGGCTCGATAGAGAAGATGGCTGATAATGCTGTTACTGCGGACGTACTGGGTATAATAACCGAGAGAAATGAGATTTTGACACTGGATAATTGTACCTCCCTTCTTCCCCTTCTAACAGCTCTTTTAGGGAGCGGCATGGATCAGCATTTGAGCGTTTCCCTGGATTTGCTGCTGAAGCTTGTCAGACTGTATGGGTCACCAATTTACTCATCACTGTCAGCTCCAGCATCTGTTGGTGTAGATATCGAGGCTGAGCAAAGGATCGAGCGCTACAGCCGTTGCTTTGTAGAGCTAGAGAAAGTCAAAGCATGCCTTCCCTCTCTAGCAAGAAGAGGAGGTTTGGTGGCCAAGTCTGTGCTCGAACTCAACCTAGCATTTCAGGAAGTTTCATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0031461 GO:0032991 GO:0044424 GO:0044464 GO:0080008 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Katanin_con80 WD40

Protein Analysis

837

Amino Acids

91.43

Weight (kDa)

8.84

Isoelectric Point (pI)

49.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_MABP1-WDR62_2nd PF24782 7 - 172 2.2e-12 MABP1/WDR62 second WD40 domain
Beta-prop_TEP1_2nd PF25047 9 - 130 1.2e-10 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 10 - 138 2e-25 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 10 - 130 2.1e-22 WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 11 - 128 6.6e-07 WDR36/Utp21 first beta-propeller
WD40_Gbeta PF25391 11 - 132 4.8e-13 G protein beta WD-40 repeat protein
Beta-prop_WDR36-Utp21_2nd PF25168 13 - 137 8e-13 WDR36/Utp21 second beta-propeller domain
WD40_Prp19 PF24814 15 - 255 1e-46 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 16 - 97 6e-10 WDHD1 first WD40 domain
Beta-prop_SCAP PF24017 28 - 129 1.4e-09 SCAP Beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 28 - 130 1.2e-06 WDR90/POC16, second beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 34 - 170 1.7e-12 WDR36/Utp21 first beta-propeller
WD40_CDC20-Fz PF24807 52 - 245 2.4e-31 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_2nd PF25172 52 - 174 7.2e-19 WDR3 second beta-propeller domain
Beta-prop_THOC3 PF25174 53 - 196 6.3e-32 THOC3 beta-propeller domain
WDR55 PF24796 62 - 258 1.2e-21 WDR55
Beta-prop_EML_2 PF23414 71 - 193 6.2e-14 Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st PF24817 73 - 141 4.7e-12 WDHD1 first WD40 domain
WD40 PF00400 92 - 129 3.3e-09 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 96 - 260 8.5e-35 WDR5 beta-propeller domain
Beta-prop_Aladin PF25460 102 - 196 2.6e-08 Aladin seven-bladed propeller
Beta-prop_WDR3_1st PF25173 115 - 250 4.5e-29 WDR3 first beta-propeller domain
Beta-prop_SPT8 PF23798 117 - 197 2.3e-07 SPT8 beta propeller
WD40_Gbeta PF25391 120 - 260 1.4e-14 G protein beta WD-40 repeat protein
Beta-prop_WDR36-Utp21_2nd PF25168 127 - 202 3.3e-11 WDR36/Utp21 second beta-propeller domain
WD40 PF00400 135 - 171 9.1e-12 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 137 - 217 6.2e-10 CAF1B/HIR1 beta-propeller domain
EIF3I PF24805 140 - 249 1e-14 EIF3I
WD40_MABP1-WDR62_2nd PF24782 142 - 215 6e-07 MABP1/WDR62 second WD40 domain
WD40_WDHD1_1st PF24817 144 - 218 2.1e-11 WDHD1 first WD40 domain
Beta-prop_WDR90_POC16_2nd PF23393 163 - 243 4.5e-07 WDR90/POC16, second beta-propeller
Beta-prop_WDR3_2nd PF25172 168 - 218 4.7e-07 WDR3 second beta-propeller domain
WD40 PF00400 175 - 213 5e-09 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 176 - 247 1.6e-11 THOC3 beta-propeller domain
Beta-prop_EML_2 PF23414 190 - 295 2.1e-07 Echinoderm microtubule-associated protein second beta-propeller
Katanin_con80 PF13925 675 - 830 7.6e-48 con80 domain of Katanin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 930
AccBSI CCGCTC 1 cut(s) 2250
AccII CGCG 2 cut(s) 430, 898
AciI CCGC 5 cut(s) 1508, 1651, 1924, 2156, 2250
AclWI GGATC 7 cut(s) 645, 968, 1037, 1094, 1985, 2265, 2387
AcoI YGGCCR 1 cut(s) 2463
AcsI RAATTY 9 cut(s) 32, 561, 590, 629, 1150, 1207, 1351, 1888, 2110
AcuI CTGAAG 3 cut(s) 11, 2013, 2312
AfaI GTAC 6 cut(s) 244, 444, 901, 1624, 2163, 2212
AfeI AGCGCT 1 cut(s) 2387
AfiI CCNNNNNNNGG 2 cut(s) 1131, 1619
AflII CTTAAG 2 cut(s) 65, 374
AjnI CCWGG 3 cut(s) 1258, 1630, 2277
AleI CACNNNNGTG 1 cut(s) 177
Alw21I GWGCWC 1 cut(s) 2478
Alw26I GTCTC 8 cut(s) 671, 752, 915, 1071, 1330, 1590, 1779, 2058
AlwI GGATC 7 cut(s) 645, 968, 1037, 1094, 1985, 2265, 2387
AlwNI CAGNNNCTG 2 cut(s) 233, 2348
Ama87I CYCGRG 2 cut(s) 1013, 1184
Aor51HI AGCGCT 1 cut(s) 2387
AoxI GGCC 1 cut(s) 2463
ApeKI GCWGC 8 cut(s) 50, 224, 233, 402, 1334, 1394, 1533, 2287
ApoI RAATTY 9 cut(s) 32, 561, 590, 629, 1150, 1207, 1351, 1888, 2110
AseI ATTAAT 1 cut(s) 1674
Asp700I GAANNNNTTC 2 cut(s) 187, 1861
AspLEI GCGC 2 cut(s) 931, 2388
AspS9I GGNCC 4 cut(s) 303, 671, 688, 1877
AsuC2I CCSGG 1 cut(s) 685
AsuHPI GGTGA 7 cut(s) 119, 350, 445, 1423, 1518, 1722, 2307
AvaI CYCGRG 2 cut(s) 1013, 1184
AvaII GGWCC 4 cut(s) 303, 671, 688, 1877
BaeGI GKGCMC 1 cut(s) 174
BaeI ACNNNNGTAYC 2 cut(s) 1275, 1308
BalI TGGCCA 1 cut(s) 2465
BauI CACGAG 1 cut(s) 830
BbsI GAAGAC 2 cut(s) 75, 2016
Bbv12I GWGCWC 1 cut(s) 2478
BbvI GCAGC 8 cut(s) 37, 220, 236, 389, 1321, 1406, 1545, 2274
BccI CCATC 7 cut(s) 455, 707, 779, 795, 1610, 1717, 2128
BceAI ACGGC 3 cut(s) 1307, 1477, 2379
BciT130I CCWGG 3 cut(s) 1260, 1632, 2279
BcnI CCSGG 1 cut(s) 685
BcoDI GTCTC 8 cut(s) 671, 752, 915, 1071, 1330, 1590, 1779, 2058
BfmI CTRYAG 1 cut(s) 2389
BfoI RGCGCY 1 cut(s) 2389
BfrI CTTAAG 2 cut(s) 65, 374
BglII AGATCT 1 cut(s) 889
BlpI GCTNAGC 1 cut(s) 2370
Bme1390I CCNGG 4 cut(s) 685, 1260, 1632, 2279
Bme18I GGWCC 4 cut(s) 303, 671, 688, 1877
BmeT110I CYCGRG 2 cut(s) 1013, 1184
BmgT120I GGNCC 4 cut(s) 303, 671, 688, 1877
BmiI GGNNCC 4 cut(s) 689, 768, 1878, 1955
BmrFI CCNGG 4 cut(s) 685, 1260, 1632, 2279
BmrI ACTGGG 1 cut(s) 2174
BmsI GCATC 4 cut(s) 193, 321, 414, 2354
BmuI ACTGGG 1 cut(s) 2174
BpiI GAAGAC 2 cut(s) 75, 2016
BpmI CTGGAG 3 cut(s) 501, 939, 2325
Bpu10I CCTNAGC 1 cut(s) 964
Bpu1102I GCTNAGC 1 cut(s) 2370
BpuEI CTTGAG 2 cut(s) 160, 734
BpuMI CCSGG 1 cut(s) 685
Bsa29I ATCGAT 1 cut(s) 1980
BsaBI GATNNNNATC 1 cut(s) 579
BsaI GGTCTC 1 cut(s) 1779
BsaJI CCNNGG 2 cut(s) 684, 2277
BsaWI WCCGGW 1 cut(s) 1408
BsaXI ACNNNNNCTCC 6 cut(s) 426, 456, 780, 810, 2445, 2475
Bsc4I CCNNNNNNNGG 2 cut(s) 1131, 1619
Bse1I ACTGG 8 cut(s) 298, 603, 977, 1592, 1825, 1964, 2169, 2205
Bse3DI GCAATG 1 cut(s) 1499
Bse8I GATNNNNATC 1 cut(s) 579
BseBI CCWGG 3 cut(s) 1260, 1632, 2279
BseCI ATCGAT 1 cut(s) 1980
BseDI CCNNGG 2 cut(s) 684, 2277
BseGI GGATG 8 cut(s) 405, 580, 799, 806, 943, 1241, 1728, 1920
BseJI GATNNNNATC 1 cut(s) 579
BseLI CCNNNNNNNGG 2 cut(s) 1131, 1619
BseMI GCAATG 1 cut(s) 1499
BseNI ACTGG 8 cut(s) 298, 603, 977, 1592, 1825, 1964, 2169, 2205
BseRI GAGGAG 6 cut(s) 281, 447, 498, 1257, 1864, 2466
BseSI GKGCMC 1 cut(s) 174
BseXI GCAGC 8 cut(s) 37, 220, 236, 389, 1321, 1406, 1545, 2274
BseYI CCCAGC 1 cut(s) 468
Bsh1236I CGCG 2 cut(s) 430, 898
BshFI GGCC 1 cut(s) 2465
BshVI ATCGAT 1 cut(s) 1980
BsiHKAI GWGCWC 1 cut(s) 2478
BsiHKCI CYCGRG 2 cut(s) 1013, 1184
BsiSI CCGG 2 cut(s) 684, 1409
BslFI GGGAC 1 cut(s) 1403
BslI CCNNNNNNNGG 2 cut(s) 1131, 1619
BsmAI GTCTC 8 cut(s) 671, 752, 915, 1071, 1330, 1590, 1779, 2058
BsmBI CGTCTC 1 cut(s) 1330
BsmFI GGGAC 1 cut(s) 1403
BsnI GGCC 1 cut(s) 2465
Bso31I GGTCTC 1 cut(s) 1779
BsoBI CYCGRG 2 cut(s) 1013, 1184
Bsp1286I GDGCHC 2 cut(s) 174, 2478
Bsp1720I GCTNAGC 1 cut(s) 2370
BspACI CCGC 5 cut(s) 1508, 1651, 1924, 2156, 2250
BspANI GGCC 1 cut(s) 2465
BspDI ATCGAT 1 cut(s) 1980
BspFNI CGCG 2 cut(s) 430, 898
BspHI TCATGA 3 cut(s) 550, 586, 781
BspLI GGNNCC 4 cut(s) 689, 768, 1878, 1955
BspPI GGATC 7 cut(s) 645, 968, 1037, 1094, 1985, 2265, 2387
BspTI CTTAAG 2 cut(s) 65, 374
BspTNI GGTCTC 1 cut(s) 1779
BsrBI CCGCTC 1 cut(s) 2250
BsrDI GCAATG 1 cut(s) 1499
BsrI ACTGG 8 cut(s) 298, 603, 977, 1592, 1825, 1964, 2169, 2205
BssECI CCNNGG 2 cut(s) 684, 2277
BssSI CACGAG 1 cut(s) 830
Bst2BI CACGAG 1 cut(s) 830
Bst2UI CCWGG 3 cut(s) 1260, 1632, 2279
Bst4CI ACNGT 5 cut(s) 625, 724, 1009, 2307, 2334
Bst6I CTCTTC 2 cut(s) 964, 2443
BstAFI CTTAAG 2 cut(s) 65, 374
BstAPI GCANNNNNTGC 3 cut(s) 47, 233, 1403
BstC8I GCNNGC 6 cut(s) 15, 1446, 1506, 1649, 1911, 2428
BstEII GGTNACC 3 cut(s) 1524, 1728, 2313
BstF5I GGATG 8 cut(s) 405, 580, 799, 806, 943, 1241, 1728, 1920
BstFNI CGCG 2 cut(s) 430, 898
BstH2I RGCGCY 1 cut(s) 2389
BstHHI GCGC 2 cut(s) 931, 2388
BstMAI GTCTC 8 cut(s) 671, 752, 915, 1071, 1330, 1590, 1779, 2058
BstMWI GCNNNNNNNGC 8 cut(s) 47, 230, 233, 904, 1403, 2144, 2153, 2293
BstNI CCWGG 3 cut(s) 1260, 1632, 2279
BstNSI RCATGY 2 cut(s) 1651, 2430
BstPI GGTNACC 3 cut(s) 1524, 1728, 2313
BstSCI CCNGG 4 cut(s) 683, 1258, 1630, 2277
BstSFI CTRYAG 1 cut(s) 2389
BstSLI GKGCMC 1 cut(s) 174
BstUI CGCG 2 cut(s) 430, 898
BstV1I GCAGC 8 cut(s) 37, 220, 236, 389, 1321, 1406, 1545, 2274
BstV2I GAAGAC 2 cut(s) 75, 2016
BstX2I RGATCY 4 cut(s) 637, 889, 973, 1042
BstXI CCANNNNNNTGG 1 cut(s) 335
BstYI RGATCY 4 cut(s) 637, 889, 973, 1042
Bsu15I ATCGAT 1 cut(s) 1980
BsuRI GGCC 1 cut(s) 2465
BsuTUI ATCGAT 1 cut(s) 1980
BtsCI GGATG 8 cut(s) 405, 580, 799, 806, 943, 1241, 1728, 1920
BtsI GCAGTG 1 cut(s) 924
BtsIMutI CAGTG 7 cut(s) 198, 291, 324, 924, 1797, 2198, 2330
Cac8I GCNNGC 6 cut(s) 15, 1446, 1506, 1649, 1911, 2428
CaiI CAGNNNCTG 2 cut(s) 233, 2348
CciI TCATGA 3 cut(s) 550, 586, 781
CfoI GCGC 2 cut(s) 931, 2388
Cfr13I GGNCC 4 cut(s) 303, 671, 688, 1877
ClaI ATCGAT 1 cut(s) 1980
CseI GACGC 2 cut(s) 339, 1568
Csp6I GTAC 6 cut(s) 243, 443, 900, 1623, 2162, 2211
CviAII CATG 9 cut(s) 551, 587, 762, 782, 909, 1489, 1648, 2254, 2427
CviQI GTAC 6 cut(s) 243, 443, 900, 1623, 2162, 2211
EaeI YGGCCR 1 cut(s) 2463
Eam1104I CTCTTC 2 cut(s) 964, 2443
EarI CTCTTC 2 cut(s) 964, 2443
EciI GGCGGA 1 cut(s) 1913
Eco31I GGTCTC 1 cut(s) 1779
Eco32I GATATC 1 cut(s) 2362
Eco47I GGWCC 4 cut(s) 303, 671, 688, 1877
Eco47III AGCGCT 1 cut(s) 2387
Eco57I CTGAAG 3 cut(s) 11, 2013, 2312
Eco88I CYCGRG 2 cut(s) 1013, 1184
Eco91I GGTNACC 3 cut(s) 1524, 1728, 2313
EcoO109I RGGNCCY 1 cut(s) 1877
EcoO65I GGTNACC 3 cut(s) 1524, 1728, 2313
EcoRI GAATTC 1 cut(s) 590
EcoRII CCWGG 3 cut(s) 1258, 1630, 2277
EcoRV GATATC 1 cut(s) 2362
Esp3I CGTCTC 1 cut(s) 1330
FaeI CATG 9 cut(s) 554, 590, 765, 785, 912, 1492, 1651, 2257, 2430
FalI AAGNNNNNCTT 4 cut(s) 67, 99, 2415, 2447
FaqI GGGAC 1 cut(s) 1403
FatI CATG 9 cut(s) 550, 586, 761, 781, 908, 1488, 1647, 2253, 2426
FauI CCCGC 1 cut(s) 1644
FauNDI CATATG 1 cut(s) 1959
FokI GGATG 8 cut(s) 392, 567, 806, 813, 930, 1228, 1735, 1907
FspI TGCGCA 1 cut(s) 930
GlaI GCGC 2 cut(s) 930, 2387
GsaI CCCAGC 1 cut(s) 472
GsuI CTGGAG 3 cut(s) 501, 939, 2325
HaeII RGCGCY 1 cut(s) 2389
HaeIII GGCC 1 cut(s) 2465
HapII CCGG 2 cut(s) 684, 1409
HgaI GACGC 2 cut(s) 339, 1568
HhaI GCGC 2 cut(s) 931, 2388
Hin1II CATG 9 cut(s) 554, 590, 765, 785, 912, 1492, 1651, 2257, 2430
Hin6I GCGC 2 cut(s) 929, 2386
HinP1I GCGC 2 cut(s) 929, 2386
HindIII AAGCTT 3 cut(s) 23, 839, 2294
HpaII CCGG 2 cut(s) 684, 1409
HphI GGTGA 7 cut(s) 119, 350, 445, 1423, 1518, 1722, 2307
Hpy166II GTNNAC 4 cut(s) 671, 829, 1256, 1625
Hpy8I GTNNAC 4 cut(s) 671, 829, 1256, 1625
HpyCH4III ACNGT 5 cut(s) 625, 724, 1009, 2307, 2334
HpyCH4IV ACGT 5 cut(s) 197, 1215, 1235, 1340, 2160
HpyCH4V TGCA 9 cut(s) 224, 312, 405, 739, 1337, 1481, 1504, 1658, 2050
HpyF10VI GCNNNNNNNGC 8 cut(s) 47, 230, 233, 904, 1403, 2144, 2153, 2293
HpySE526I ACGT 5 cut(s) 197, 1215, 1235, 1340, 2160
Hsp92II CATG 9 cut(s) 554, 590, 765, 785, 912, 1492, 1651, 2257, 2430
HspAI GCGC 2 cut(s) 929, 2386
LmnI GCTCC 6 cut(s) 230, 766, 1411, 1918, 2247, 2344
Lsp1109I GCAGC 8 cut(s) 37, 220, 236, 389, 1321, 1406, 1545, 2274
LweI GCATC 4 cut(s) 193, 321, 414, 2354
MaeII ACGT 5 cut(s) 197, 1215, 1235, 1340, 2160
MbiI CCGCTC 1 cut(s) 2250
MfeI CAATTG 2 cut(s) 58, 307
MflI RGATCY 4 cut(s) 637, 889, 973, 1042
MhlI GDGCHC 2 cut(s) 174, 2478
MlsI TGGCCA 1 cut(s) 2465
MluNI TGGCCA 1 cut(s) 2465
MlyI GAGTC 6 cut(s) 280, 444, 556, 1166, 1235, 1592
MmeI TCCRAC 1 cut(s) 778
Mox20I TGGCCA 1 cut(s) 2465
MroXI GAANNNNTTC 2 cut(s) 187, 1861
MscI TGGCCA 1 cut(s) 2465
MseI TTAA 6 cut(s) 66, 375, 501, 515, 543, 1674
MslI CAYNNNNRTG 3 cut(s) 177, 317, 585
Msp20I TGGCCA 1 cut(s) 2465
MspA1I CMGCKG 2 cut(s) 521, 614
MspCI CTTAAG 2 cut(s) 65, 374
MspI CCGG 2 cut(s) 684, 1409
MspR9I CCNGG 4 cut(s) 685, 1260, 1632, 2279
MunI CAATTG 2 cut(s) 58, 307
MvaI CCWGG 3 cut(s) 1260, 1632, 2279
MvnI CGCG 2 cut(s) 430, 898
MwoI GCNNNNNNNGC 8 cut(s) 47, 230, 233, 904, 1403, 2144, 2153, 2293
NciI CCSGG 1 cut(s) 685
NdeI CATATG 1 cut(s) 1959
NlaIII CATG 9 cut(s) 554, 590, 765, 785, 912, 1492, 1651, 2257, 2430
NlaIV GGNNCC 4 cut(s) 689, 768, 1878, 1955
NmuCI GTSAC 8 cut(s) 193, 296, 422, 1524, 1716, 1728, 1827, 2313
NsbI TGCGCA 1 cut(s) 930
NspI RCATGY 2 cut(s) 1651, 2430
OliI CACNNNNGTG 1 cut(s) 177
PaeI GCATGC 2 cut(s) 1651, 2430
PagI TCATGA 3 cut(s) 550, 586, 781
PcsI WCGNNNNNNNCGW 1 cut(s) 2120
PdmI GAANNNNTTC 2 cut(s) 187, 1861
PfeI GAWTC 8 cut(s) 188, 450, 1172, 1378, 1454, 1514, 1821, 1982
PleI GAGTC 6 cut(s) 280, 443, 555, 1165, 1234, 1591
PpsI GAGTC 6 cut(s) 280, 443, 555, 1165, 1234, 1591
PpuMI RGGWCCY 1 cut(s) 1877
PshBI ATTAAT 1 cut(s) 1674
Psp5II RGGWCCY 1 cut(s) 1877
Psp6I CCWGG 3 cut(s) 1258, 1630, 2277
PspEI GGTNACC 3 cut(s) 1524, 1728, 2313
PspFI CCCAGC 1 cut(s) 468
PspGI CCWGG 3 cut(s) 1258, 1630, 2277
PspN4I GGNNCC 4 cut(s) 689, 768, 1878, 1955
PspPI GGNCC 4 cut(s) 303, 671, 688, 1877
PspPPI RGGWCCY 1 cut(s) 1877
PstNI CAGNNNCTG 2 cut(s) 233, 2348
PsuI RGATCY 4 cut(s) 637, 889, 973, 1042
PvuII CAGCTG 2 cut(s) 521, 614
RsaI GTAC 6 cut(s) 244, 444, 901, 1624, 2163, 2212
RsaNI GTAC 6 cut(s) 243, 443, 900, 1623, 2162, 2211
RseI CAYNNNNRTG 3 cut(s) 177, 317, 585
SaqAI TTAA 6 cut(s) 66, 375, 501, 515, 543, 1674
Sau96I GGNCC 4 cut(s) 303, 671, 688, 1877
SchI GAGTC 6 cut(s) 280, 444, 556, 1166, 1235, 1592
ScrFI CCNGG 4 cut(s) 685, 1260, 1632, 2279
SduI GDGCHC 2 cut(s) 174, 2478
SfaNI GCATC 4 cut(s) 193, 321, 414, 2354
SfcI CTRYAG 1 cut(s) 2389
SinI GGWCC 4 cut(s) 303, 671, 688, 1877
SmiMI CAYNNNNRTG 3 cut(s) 177, 317, 585
SmlI CTYRAG 4 cut(s) 65, 175, 374, 749
SmoI CTYRAG 4 cut(s) 65, 175, 374, 749
SphI GCATGC 2 cut(s) 1651, 2430
SsiI CCGC 5 cut(s) 1508, 1651, 1924, 2156, 2250
SspI AATATT 1 cut(s) 2002
StyD4I CCNGG 4 cut(s) 683, 1258, 1630, 2277
TaaI ACNGT 5 cut(s) 625, 724, 1009, 2307, 2334
TaiI ACGT 5 cut(s) 200, 1218, 1238, 1343, 2163
TaqII GACCGA 1 cut(s) 551
TatI WGTACW 1 cut(s) 442
TauI GCSGC 2 cut(s) 1511, 2253
TfiI GAWTC 8 cut(s) 188, 450, 1172, 1378, 1454, 1514, 1821, 1982
Tru1I TTAA 6 cut(s) 66, 375, 501, 515, 543, 1674
Tru9I TTAA 6 cut(s) 66, 375, 501, 515, 543, 1674
TscAI CASTG 7 cut(s) 198, 298, 324, 931, 1804, 2205, 2337
TseFI GTSAC 8 cut(s) 193, 296, 422, 1524, 1716, 1728, 1827, 2313
TseI GCWGC 8 cut(s) 50, 224, 233, 402, 1334, 1394, 1533, 2287
Tsp45I GTSAC 8 cut(s) 193, 296, 422, 1524, 1716, 1728, 1827, 2313
TspDTI ATGAA 9 cut(s) 567, 569, 603, 836, 1181, 1230, 1488, 1764, 2496
TspGWI ACGGA 3 cut(s) 680, 1015, 1735
TspRI CASTG 7 cut(s) 198, 298, 324, 931, 1804, 2205, 2337
Vha464I CTTAAG 2 cut(s) 65, 374
VpaK11BI GGWCC 4 cut(s) 303, 671, 688, 1877
VspI ATTAAT 1 cut(s) 1674
XapI RAATTY 9 cut(s) 32, 561, 590, 629, 1150, 1207, 1351, 1888, 2110
XceI RCATGY 2 cut(s) 1651, 2430
XcmI CCANNNNNNNNNTGG 1 cut(s) 465
XmnI GAANNNNTTC 2 cut(s) 187, 1861
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.