Rh4DG448200

May participate in a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0005757
Physical Location & Seq
Forward (+)
64707303 .. 64716158
8856 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG448200.1

Sequence Viewer

Length: 2148 bp
ATGACTACTAAGCGCGCTTACAAATTACAGGAATTCGTAGCACATACTTCCGCCGTCAATTGCCTCAAGATTGGCAGGAAAACTTCCAGGGTTCTTGTTACTGGCGGAGAAGATCACAAGGTCAATCTTTGGGCCATTGGCAAACCCAATGCCTTACTCAGTTTGTCTGGACACACCAGTGGCATTGATTCAGTAAGCTTTGATTCTTCAGAGGTCTTAGTAGCTGCAGGTGCTGCAAGTGGTACAATTAAGCTTTGGGATTTGGAGGAGGCAAAAATTGTTCGCACCCTTACTGGTCACAGATCCAATTGTATATCTGTCGACTTCCATCCTTTTGGAGAGTTCTTTGCATCTGGCTCCCTGGACACAAATTTAAAAATATGGGATATCAGGAAGAAGGGGTGTATTCACACATACAAAGGCCACACACGAGGAGTCAATGCTATCAGGTTTACCCCAGATGGACGTTGGGTTGTTTCTGGTGGAGAGGACAACACTGTGAAGTTGTGGGATCTGACTGCTGGAAAGCTCCTGCATGATTTTAAATGTCATGAGGGCCAGATACAATGCATAGACTTTCATCCCCATGAATTCCTACTAGCAACAGGTTCGGCAGATAGGACAGTTAAATTTTGGGATCTTGAAACCTTTGAGCTAATTGGCTCCGCTGGACCTGAGACAACTGGAGTGCGTTCTTTGACTTTTAGTCCGGATGGGAGGGCCCTACTCTGTGGATTGCATGAAAGTTTGAAGGTATTCTCGTGGGAACCAATAAGATGCCATGATGCCGTGGATGTGGGATGGTCACGATTGTCAGATCTTAATGTTCATGAGGGGAAGCTTCTTGGATGCTCCTACAATCAGAGTTGTGTTGGAGTGTGGGTTGTAGACATCTCGCGCATTGAGCCATATGCAATCAGTAATGCTAGCAGATTGAATGGGCACTCAGAATCAAAACCATTGTCTGGTGGTAATCTGTCAGTACTCAATGAGAACGCTGCTAGATCTAGTTTGGGCCGGCTCTCTGTTTCCCAAAGTTCAGATTCTCTAGTGAAGGAAACAAAATCTCTTGGAAGGTTATCAGTTTCCCAAAATTCAGATCCCGCCAAGGAGCCAAAATTTTTGACATCCACTGGAAGTGTTCCTGGTACTCCTCAAAGGATCAGTCTGAACTCCAGTCCAAAAACTATTGTTGCTAGTTCAGTGTCAGTTCCTAGTGCAACAACCCTGAAAAGGAATTCCGTCAAGACCAGTGTTCCGACCTTTAACAAGTCGGATGTCATACCTGTAATTGTGCCTAGAACTAGTGTCAGGTCAGAGCTGGCCGCCGAGTCTAGAAAAGAAGCCAGTGTTGCTGGAAGAACAATGCCATTCTCTTCGCAGTCAAAGACGAATGATTTCCGAAAGTTTTCCAATACCAGAGAAGAAGTGGATAAGCCAACTGTCTCTATAGTTTCTGAATCGACGACTTCTAAGGCTAGTGAGTTAAGCAGTGCTGTAGATGGGAGCGGTTTCTCAACAGTTATAAGCTCAAGCCAAGGAATCGATACCGAAAAAAACATGAAGGATGACAGATGCATTGGACCTGGGAAGCAGGAAACAAGTTCACTGATGGAGCCAACTGCTAGCTACCAGCATGAAAGTTCTGAAACTCGAGGGCATAAGGTGATGAGAGATGCATACTCTGCTGAAGGTCAAAGAGGAGGTAGAATGAGGTCAATTGCTGTAAATTTTGAGAGAAGAGATAGATATTCTAACTATGAGGGACCTACCTCCGATTCTACCATGGTAACAGCATCTGCAATGAACCCTTTGAATGCGGTTAAGCAGAGAGGCTATCCTACATCTACTGAAAAAGAAACGGTATCTGCTAGTGATGAGGATGCTATTGCAGATCTTTTGGAAAAACATGACCAATTTGTTAGCTCCATGCAATCTCGTTCAGCCAAGTTACAGGCAGTGTATAGATATTGGCAGAGAAATGATGTTAAGGGGGTAATTGGTGCGATGGAGAAGATGTCGGATCATGCTGTCATTGCTGATGTAATTAGCATTATGGCGGACAAAATTGACATTGTCACCCTGGATGTATGCACTTGTCTTCTGCCACTCCTCACTGGCCTTCTAGAAAGCAACATGGACAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0031461 GO:0032991 GO:0044424 GO:0044464 GO:0080008 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Katanin_con80 WD40

Protein Analysis

715

Amino Acids

77.78

Weight (kDa)

7.6

Isoelectric Point (pI)

33.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR36-Utp21_1st PF25171 4 - 128 3.9e-08 WDR36/Utp21 first beta-propeller
WD40_MABP1-WDR62_2nd PF24782 7 - 172 3.9e-12 MABP1/WDR62 second WD40 domain
Beta-prop_THOC3 PF25174 7 - 90 9e-10 THOC3 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 9 - 130 2.3e-11 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 10 - 138 5.5e-26 WDR5 beta-propeller domain
WD40_Gbeta PF25391 10 - 133 1.3e-14 G protein beta WD-40 repeat protein
Beta-prop_WDR3_1st PF25173 10 - 130 1.7e-23 WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 13 - 136 3.6e-13 WDR36/Utp21 second beta-propeller domain
Beta-prop_SCAP PF24017 15 - 129 1e-09 SCAP Beta-propeller
WD40_WDHD1_1st PF24817 15 - 97 1.4e-10 WDHD1 first WD40 domain
Beta-prop_EML PF23409 15 - 174 3.4e-09 Echinoderm microtubule-associated protein first beta-propeller
WD40_Prp19 PF24814 16 - 255 4.7e-47 Prp19 WD40 domain
Beta-prop_EML_2 PF23414 28 - 155 4.1e-13 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 28 - 133 1.3e-07 WDR90/POC16, second beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 36 - 170 7.3e-13 WDR36/Utp21 first beta-propeller
WD40_CDC20-Fz PF24807 51 - 250 8.5e-34 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_2nd PF25172 52 - 174 6.9e-18 WDR3 second beta-propeller domain
WD40 PF00400 52 - 87 2.6e-06 WD domain, G-beta repeat
EIF3I PF24805 53 - 139 7.9e-06 EIF3I
Beta-prop_CAF1B_HIR1 PF24105 54 - 138 2.1e-06 CAF1B/HIR1 beta-propeller domain
WDR55 PF24796 62 - 258 1.9e-21 WDR55
Beta-prop_THOC3 PF25174 63 - 184 2.6e-29 THOC3 beta-propeller domain
Beta-prop_EIPR1 PF23609 67 - 170 8.8e-07 EIPR1 beta-propeller
WD40_WDHD1_1st PF24817 73 - 141 3.9e-12 WDHD1 first WD40 domain
WD40 PF00400 92 - 129 2.7e-09 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 96 - 256 1.1e-36 WDR5 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 96 - 184 1.1e-07 TEP-1 second beta-propeller
Beta-prop_Aladin PF25460 102 - 174 3.7e-06 Aladin seven-bladed propeller
Beta-prop_WDR3_1st PF25173 114 - 253 3.7e-30 WDR3 first beta-propeller domain
Beta-prop_SPT8 PF23798 115 - 196 6.8e-06 SPT8 beta propeller
Beta-prop_SCAP PF24017 118 - 193 5.1e-06 SCAP Beta-propeller
WD40_Gbeta PF25391 121 - 261 8.6e-18 G protein beta WD-40 repeat protein
Beta-prop_WDR36-Utp21_2nd PF25168 129 - 182 8e-09 WDR36/Utp21 second beta-propeller domain
WD40_MABP1-WDR62_1st PF24780 130 - 242 9.5e-08 MABP1/WDR62 first WD40 domain
WD40 PF00400 135 - 171 9.9e-14 WD domain, G-beta repeat
Beta-prop_TEP1_2nd PF25047 137 - 218 1.3e-07 TEP-1 second beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 137 - 218 3.3e-11 CAF1B/HIR1 beta-propeller domain
EIF3I PF24805 140 - 248 2.4e-16 EIF3I
WD40_MABP1-WDR62_2nd PF24782 142 - 215 2.5e-07 MABP1/WDR62 second WD40 domain
ANAPC4_WD40 PF12894 143 - 194 2e-06 Anaphase-promoting complex subunit 4 WD40 domain
WD40_WDHD1_1st PF24817 144 - 218 1.8e-11 WDHD1 first WD40 domain
WDR90_beta-prop_4th PF23342 146 - 252 6e-06 WDR90, 4th beta-propeller
Beta-prop_EML_2 PF23414 158 - 249 2.1e-10 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 163 - 244 4.8e-11 WDR90/POC16, second beta-propeller
Beta-prop_THOC3 PF25174 166 - 245 1.1e-15 THOC3 beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 167 - 255 1.3e-06 WDR36/Utp21 first beta-propeller
Beta-prop_WDR3_2nd PF25172 167 - 218 8.3e-07 WDR3 second beta-propeller domain
WD40 PF00400 175 - 213 8.7e-10 WD domain, G-beta repeat
Beta-prop_WDR36-Utp21_2nd PF25168 178 - 261 1.2e-06 WDR36/Utp21 second beta-propeller domain
Beta-prop_EML_2 PF23414 190 - 297 2.2e-07 Echinoderm microtubule-associated protein second beta-propeller
Katanin_con80 PF13925 636 - 715 4.2e-26 con80 domain of Katanin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1526
AarI CACCTGC 1 cut(s) 218
Acc36I ACCTGC 1 cut(s) 218
AccB7I CCANNNNNTGG 1 cut(s) 965
AccBSI CCGCTC 1 cut(s) 1509
AccI GTMKAC 2 cut(s) 321, 888
AccII CGCG 2 cut(s) 15, 898
AccIII TCCGGA 1 cut(s) 709
AciI CCGC 8 cut(s) 51, 105, 666, 1104, 1326, 1509, 1820, 2060
AclWI GGATC 6 cut(s) 297, 519, 645, 1094, 1169, 2031
AcoI YGGCCR 1 cut(s) 1323
AcsI RAATTY 8 cut(s) 32, 370, 590, 629, 1093, 1118, 1237, 1729
AcuI CTGAAG 2 cut(s) 192, 1710
AfaI GTAC 3 cut(s) 244, 984, 1150
AfiI CCNNNNNNNGG 2 cut(s) 965, 1233
AgsI TTSAA 4 cut(s) 644, 751, 937, 1816
AhdI GACNNNNNGTC 1 cut(s) 705
AhlI ACTAGT 1 cut(s) 1304
AjnI CCWGG 5 cut(s) 86, 360, 1144, 1585, 2082
AleI CACNNNNGTG 1 cut(s) 177
Alw26I GTCTC 2 cut(s) 671, 1450
AlwI GGATC 6 cut(s) 297, 519, 645, 1094, 1169, 2031
AlwNI CAGNNNCTG 2 cut(s) 233, 1799
Ama87I CYCGRG 1 cut(s) 1653
Aor13HI TCCGGA 1 cut(s) 709
AoxI GGCC 7 cut(s) 132, 421, 556, 720, 1015, 1323, 2119
ApaI GGGCCC 1 cut(s) 724
ApeKI GCWGC 3 cut(s) 224, 233, 998
ApoI RAATTY 8 cut(s) 32, 370, 590, 629, 1093, 1118, 1237, 1729
ArsI GACNNNNNNTTYG 4 cut(s) 947, 979, 1150, 1182
Asp700I GAANNNNTTC 3 cut(s) 755, 1397, 1408
AspLEI GCGC 3 cut(s) 15, 17, 900
AspS9I GGNCC 8 cut(s) 132, 556, 671, 720, 721, 1015, 1583, 1766
AsuHPI GGTGA 2 cut(s) 1678, 2071
AsuNHI GCTAGC 2 cut(s) 926, 1625
AvaI CYCGRG 1 cut(s) 1653
AvaII GGWCC 3 cut(s) 671, 1583, 1766
BaeGI GKGCMC 2 cut(s) 724, 945
BanII GRGCYC 1 cut(s) 724
BauI CACGAG 2 cut(s) 429, 760
BbsI GAAGAC 1 cut(s) 2093
BbvI GCAGC 3 cut(s) 211, 220, 985
BccI CCATC 7 cut(s) 336, 455, 707, 795, 1496, 1606, 2002
BceAI ACGGC 2 cut(s) 38, 773
BcgI CGANNNNNNTGC 2 cut(s) 591, 625
BciT130I CCWGG 5 cut(s) 88, 362, 1146, 1587, 2084
BcoDI GTCTC 2 cut(s) 671, 1450
BcuI ACTAGT 1 cut(s) 1304
BfmI CTRYAG 3 cut(s) 225, 1449, 1497
BfuAI ACCTGC 1 cut(s) 218
BglII AGATCT 3 cut(s) 817, 1004, 1894
BisI GCNGC 4 cut(s) 225, 234, 999, 1326
BlsI GCNGC 4 cut(s) 226, 235, 1000, 1327
BmcAI AGTACT 1 cut(s) 984
Bme1390I CCNGG 5 cut(s) 88, 362, 1146, 1587, 2084
Bme18I GGWCC 3 cut(s) 671, 1583, 1766
BmeRI GACNNNNNGTC 1 cut(s) 705
BmeT110I CYCGRG 1 cut(s) 1653
BmgT120I GGNCC 8 cut(s) 132, 556, 671, 720, 721, 1015, 1583, 1766
BmiI GGNNCC 7 cut(s) 358, 664, 722, 768, 1113, 1617, 1767
BmrFI CCNGG 5 cut(s) 88, 362, 1146, 1587, 2084
BmsI GCATC 8 cut(s) 359, 767, 775, 839, 1565, 1666, 1805, 1873
BmtI GCTAGC 2 cut(s) 930, 1629
BpiI GAAGAC 1 cut(s) 2093
BpmI CTGGAG 2 cut(s) 705, 1159
BpuEI CTTGAG 2 cut(s) 50, 1516
Bsa29I ATCGAT 1 cut(s) 1545
BsaJI CCNNGG 8 cut(s) 87, 360, 789, 1107, 1537, 1586, 1785, 2082
BsaWI WCCGGW 1 cut(s) 709
BsaXI ACNNNNNCTCC 4 cut(s) 867, 897, 2003, 2033
Bsc4I CCNNNNNNNGG 2 cut(s) 965, 1233
Bse118I RCCGGY 1 cut(s) 1017
Bse1I ACTGG 9 cut(s) 106, 177, 298, 688, 1138, 1176, 1251, 1347, 2122
Bse3DI GCAATG 2 cut(s) 1809, 2034
BseAI TCCGGA 1 cut(s) 709
BseBI CCWGG 5 cut(s) 88, 362, 1146, 1587, 2084
BseCI ATCGAT 1 cut(s) 1545
BseDI CCNNGG 8 cut(s) 87, 360, 789, 1107, 1537, 1586, 1785, 2082
BseLI CCNNNNNNNGG 2 cut(s) 965, 1233
BseMI GCAATG 2 cut(s) 1809, 2034
BseMII CTCAG 3 cut(s) 172, 666, 960
BseNI ACTGG 9 cut(s) 106, 177, 298, 688, 1138, 1176, 1251, 1347, 2122
BsePI GCGCGC 1 cut(s) 13
BseRI GAGGAG 5 cut(s) 281, 447, 1143, 1716, 2102
BseSI GKGCMC 2 cut(s) 724, 945
BseXI GCAGC 3 cut(s) 211, 220, 985
Bsh1236I CGCG 2 cut(s) 15, 898
BshFI GGCC 7 cut(s) 134, 423, 558, 722, 1017, 1325, 2121
BshVI ATCGAT 1 cut(s) 1545
BsiHKCI CYCGRG 1 cut(s) 1653
BsiSI CCGG 2 cut(s) 710, 1018
BslFI GGGAC 1 cut(s) 1779
BslI CCNNNNNNNGG 2 cut(s) 965, 1233
BsmAI GTCTC 2 cut(s) 671, 1450
BsmFI GGGAC 1 cut(s) 1779
BsmI GAATGC 1 cut(s) 1822
BsnI GGCC 7 cut(s) 134, 423, 558, 722, 1017, 1325, 2121
BsoBI CYCGRG 1 cut(s) 1653
Bsp120I GGGCCC 1 cut(s) 720
Bsp1286I GDGCHC 2 cut(s) 724, 945
Bsp13I TCCGGA 1 cut(s) 709
Bsp19I CCATGG 1 cut(s) 1785
BspACI CCGC 8 cut(s) 51, 105, 666, 1104, 1326, 1509, 1820, 2060
BspANI GGCC 7 cut(s) 134, 423, 558, 722, 1017, 1325, 2121
BspCNI CTCAG 3 cut(s) 171, 667, 959
BspDI ATCGAT 1 cut(s) 1545
BspEI TCCGGA 1 cut(s) 709
BspFNI CGCG 2 cut(s) 15, 898
BspHI TCATGA 2 cut(s) 550, 829
BspLI GGNNCC 7 cut(s) 358, 664, 722, 768, 1113, 1617, 1767
BspMAI CTGCAG 1 cut(s) 229
BspMI ACCTGC 1 cut(s) 218
BspOI GCTAGC 2 cut(s) 930, 1629
BspPI GGATC 6 cut(s) 297, 519, 645, 1094, 1169, 2031
BsrBI CCGCTC 1 cut(s) 1509
BsrDI GCAATG 2 cut(s) 1809, 2034
BsrFI RCCGGY 1 cut(s) 1017
BsrI ACTGG 9 cut(s) 106, 177, 298, 688, 1138, 1176, 1251, 1347, 2122
BssAI RCCGGY 1 cut(s) 1017
BssECI CCNNGG 8 cut(s) 87, 360, 789, 1107, 1537, 1586, 1785, 2082
BssHII GCGCGC 1 cut(s) 13
BssSI CACGAG 2 cut(s) 429, 760
BssT1I CCWWGG 3 cut(s) 1107, 1537, 1785
Bst2BI CACGAG 2 cut(s) 429, 760
Bst2UI CCWGG 5 cut(s) 88, 362, 1146, 1587, 2084
Bst4CI ACNGT 5 cut(s) 499, 625, 1444, 1522, 1864
Bst6I CTCTTC 2 cut(s) 1381, 1735
BstAPI GCANNNNNTGC 2 cut(s) 233, 1685
BstC8I GCNNGC 5 cut(s) 15, 928, 1019, 1323, 1627
BstDEI CTNAG 6 cut(s) 9, 158, 217, 675, 946, 1473
BstDSI CCRYGG 2 cut(s) 789, 1785
BstFNI CGCG 2 cut(s) 15, 898
BstHHI GCGC 3 cut(s) 15, 17, 900
BstMAI GTCTC 2 cut(s) 671, 1450
BstMWI GCNNNNNNNGC 6 cut(s) 230, 233, 904, 1352, 1685, 2036
BstNI CCWGG 5 cut(s) 88, 362, 1146, 1587, 2084
BstSCI CCNGG 5 cut(s) 86, 360, 1144, 1585, 2082
BstSFI CTRYAG 3 cut(s) 225, 1449, 1497
BstSLI GKGCMC 2 cut(s) 724, 945
BstUI CGCG 2 cut(s) 15, 898
BstV1I GCAGC 3 cut(s) 211, 220, 985
BstV2I GAAGAC 1 cut(s) 2093
BstX2I RGATCY 7 cut(s) 302, 511, 637, 817, 1004, 1099, 1894
BstXI CCANNNNNNTGG 1 cut(s) 335
BstYI RGATCY 7 cut(s) 302, 511, 637, 817, 1004, 1099, 1894
Bsu15I ATCGAT 1 cut(s) 1545
BsuRI GGCC 7 cut(s) 134, 423, 558, 722, 1017, 1325, 2121
BsuTUI ATCGAT 1 cut(s) 1545
BtgI CCRYGG 2 cut(s) 789, 1785
BtgZI GCGATG 1 cut(s) 2021
BtsI GCAGTG 2 cut(s) 1498, 1965
BveI ACCTGC 1 cut(s) 218
Cac8I GCNNGC 5 cut(s) 15, 928, 1019, 1323, 1627
CaiI CAGNNNCTG 2 cut(s) 233, 1799
CciI TCATGA 2 cut(s) 550, 829
CfoI GCGC 3 cut(s) 15, 17, 900
Cfr10I RCCGGY 1 cut(s) 1017
Cfr13I GGNCC 8 cut(s) 132, 556, 671, 720, 721, 1015, 1583, 1766
ClaI ATCGAT 1 cut(s) 1545
Csp6I GTAC 3 cut(s) 243, 983, 1149
CviQI GTAC 3 cut(s) 243, 983, 1149
DdeI CTNAG 6 cut(s) 9, 158, 217, 675, 946, 1473
DraI TTTAAA 2 cut(s) 375, 544
DriI GACNNNNNGTC 1 cut(s) 705
EaeI YGGCCR 1 cut(s) 1323
Eam1104I CTCTTC 2 cut(s) 1381, 1735
Eam1105I GACNNNNNGTC 1 cut(s) 705
EarI CTCTTC 2 cut(s) 1381, 1735
EciI GGCGGA 3 cut(s) 40, 120, 2075
Eco130I CCWWGG 3 cut(s) 1107, 1537, 1785
Eco24I GRGCYC 1 cut(s) 724
Eco32I GATATC 1 cut(s) 388
Eco47I GGWCC 3 cut(s) 671, 1583, 1766
Eco57I CTGAAG 2 cut(s) 192, 1710
Eco88I CYCGRG 1 cut(s) 1653
EcoO109I RGGNCCY 3 cut(s) 720, 721, 1766
EcoRI GAATTC 3 cut(s) 32, 590, 1237
EcoRII CCWGG 5 cut(s) 86, 360, 1144, 1585, 2082
EcoRV GATATC 1 cut(s) 388
EcoT14I CCWWGG 3 cut(s) 1107, 1537, 1785
EcoT22I ATGCAT 3 cut(s) 572, 1580, 1681
EcoT38I GRGCYC 1 cut(s) 724
ErhI CCWWGG 3 cut(s) 1107, 1537, 1785
FaqI GGGAC 1 cut(s) 1779
FauI CCCGC 1 cut(s) 1111
FauNDI CATATG 1 cut(s) 910
FblI GTMKAC 2 cut(s) 321, 888
Fnu4HI GCNGC 4 cut(s) 225, 234, 999, 1326
FriOI GRGCYC 1 cut(s) 724
Fsp4HI GCNGC 4 cut(s) 225, 234, 999, 1326
GlaI GCGC 3 cut(s) 14, 16, 899
GluI GCNGC 4 cut(s) 225, 234, 999, 1326
GsuI CTGGAG 2 cut(s) 705, 1159
HaeIII GGCC 7 cut(s) 134, 423, 558, 722, 1017, 1325, 2121
HapII CCGG 2 cut(s) 710, 1018
HhaI GCGC 3 cut(s) 15, 17, 900
Hin6I GCGC 3 cut(s) 13, 15, 898
HinP1I GCGC 3 cut(s) 13, 15, 898
HincII GTYRAC 1 cut(s) 322
HindII GTYRAC 1 cut(s) 322
HindIII AAGCTT 3 cut(s) 196, 251, 839
HinfI GANTC 9 cut(s) 188, 203, 435, 950, 1043, 1331, 1460, 1542, 1778
HpaII CCGG 2 cut(s) 710, 1018
HphI GGTGA 2 cut(s) 1678, 2071
Hpy166II GTNNAC 4 cut(s) 322, 453, 889, 1607
Hpy8I GTNNAC 4 cut(s) 322, 453, 889, 1607
Hpy99I CGWCG 1 cut(s) 1468
HpyAV CCTTC 7 cut(s) 391, 745, 1048, 1068, 1558, 1685, 2132
HpyCH4III ACNGT 5 cut(s) 499, 625, 1444, 1522, 1864
HpyCH4IV ACGT 1 cut(s) 466
HpyF10VI GCNNNNNNNGC 6 cut(s) 230, 233, 904, 1352, 1685, 2036
HpyF3I CTNAG 6 cut(s) 9, 158, 217, 675, 946, 1473
HpySE526I ACGT 1 cut(s) 466
HspAI GCGC 3 cut(s) 13, 15, 898
Kpn2I TCCGGA 1 cut(s) 709
KroI GCCGGC 1 cut(s) 1017
KroNI GCCGGC 1 cut(s) 1019
LmnI GCTCC 8 cut(s) 362, 534, 668, 857, 1111, 1506, 1615, 1931
Lsp1109I GCAGC 3 cut(s) 211, 220, 985
LweI GCATC 8 cut(s) 359, 767, 775, 839, 1565, 1666, 1805, 1873
MaeII ACGT 1 cut(s) 466
MaeIII GTNAC 6 cut(s) 97, 296, 804, 1789, 1950, 2077
MbiI CCGCTC 1 cut(s) 1509
MboII GAAGA 9 cut(s) 122, 198, 406, 1368, 1371, 1436, 1752, 2026, 2093
MfeI CAATTG 3 cut(s) 58, 307, 1719
MflI RGATCY 7 cut(s) 302, 511, 637, 817, 1004, 1099, 1894
MhlI GDGCHC 2 cut(s) 724, 945
MlyI GAGTC 2 cut(s) 444, 1340
MmeI TCCRAC 4 cut(s) 853, 1254, 1283, 2001
Mph1103I ATGCAT 3 cut(s) 572, 1580, 1681
MroI TCCGGA 1 cut(s) 709
MroNI GCCGGC 1 cut(s) 1017
MroXI GAANNNNTTC 3 cut(s) 755, 1397, 1408
MseI TTAA 9 cut(s) 249, 374, 543, 627, 822, 1266, 1487, 1824, 1989
MslI CAYNNNNRTG 2 cut(s) 177, 585
MspA1I CMGCKG 1 cut(s) 668
MspI CCGG 2 cut(s) 710, 1018
MspR9I CCNGG 5 cut(s) 88, 362, 1146, 1587, 2084
MunI CAATTG 3 cut(s) 58, 307, 1719
Mva1269I GAATGC 1 cut(s) 1822
MvaI CCWGG 5 cut(s) 88, 362, 1146, 1587, 2084
MvnI CGCG 2 cut(s) 15, 898
MwoI GCNNNNNNNGC 6 cut(s) 230, 233, 904, 1352, 1685, 2036
NaeI GCCGGC 1 cut(s) 1019
NcoI CCATGG 1 cut(s) 1785
NdeI CATATG 1 cut(s) 910
NgoMIV GCCGGC 1 cut(s) 1017
NheI GCTAGC 2 cut(s) 926, 1625
NlaIV GGNNCC 7 cut(s) 358, 664, 722, 768, 1113, 1617, 1767
NmeAIII GCCGAG 1 cut(s) 1354
NmuCI GTSAC 3 cut(s) 296, 804, 2077
NsiI ATGCAT 3 cut(s) 572, 1580, 1681
OliI CACNNNNGTG 1 cut(s) 177
PaeR7I CTCGAG 1 cut(s) 1653
PagI TCATGA 2 cut(s) 550, 829
PaqCI CACCTGC 1 cut(s) 218
PauI GCGCGC 1 cut(s) 13
PctI GAATGC 1 cut(s) 1822
PdiI GCCGGC 1 cut(s) 1019
PdmI GAANNNNTTC 3 cut(s) 755, 1397, 1408
PfeI GAWTC 7 cut(s) 188, 203, 950, 1043, 1460, 1542, 1778
PflFI GACNNNGTC 1 cut(s) 2075
PflMI CCANNNNNTGG 1 cut(s) 965
PkrI GCNGC 4 cut(s) 226, 235, 1000, 1327
PleI GAGTC 2 cut(s) 443, 1339
PpsI GAGTC 2 cut(s) 443, 1339
PpuMI RGGWCCY 1 cut(s) 1766
PsiI TTATAA 1 cut(s) 1526
Psp5II RGGWCCY 1 cut(s) 1766
Psp6I CCWGG 5 cut(s) 86, 360, 1144, 1585, 2082
PspGI CCWGG 5 cut(s) 86, 360, 1144, 1585, 2082
PspN4I GGNNCC 7 cut(s) 358, 664, 722, 768, 1113, 1617, 1767
PspOMI GGGCCC 1 cut(s) 720
PspPI GGNCC 8 cut(s) 132, 556, 671, 720, 721, 1015, 1583, 1766
PspPPI RGGWCCY 1 cut(s) 1766
PspXI VCTCGAGB 1 cut(s) 1653
PstI CTGCAG 1 cut(s) 229
PstNI CAGNNNCTG 2 cut(s) 233, 1799
PsuI RGATCY 7 cut(s) 302, 511, 637, 817, 1004, 1099, 1894
PsyI GACNNNGTC 1 cut(s) 2075
PteI GCGCGC 1 cut(s) 13
RsaI GTAC 3 cut(s) 244, 984, 1150
RsaNI GTAC 3 cut(s) 243, 983, 1149
RseI CAYNNNNRTG 2 cut(s) 177, 585
SalI GTCGAC 1 cut(s) 320
SaqAI TTAA 9 cut(s) 249, 374, 543, 627, 822, 1266, 1487, 1824, 1989
SatI GCNGC 4 cut(s) 225, 234, 999, 1326
Sau96I GGNCC 8 cut(s) 132, 556, 671, 720, 721, 1015, 1583, 1766
ScaI AGTACT 1 cut(s) 984
SchI GAGTC 2 cut(s) 444, 1340
ScrFI CCNGG 5 cut(s) 88, 362, 1146, 1587, 2084
SduI GDGCHC 2 cut(s) 724, 945
SfaNI GCATC 8 cut(s) 359, 767, 775, 839, 1565, 1666, 1805, 1873
SfcI CTRYAG 3 cut(s) 225, 1449, 1497
Sfr274I CTCGAG 1 cut(s) 1653
SinI GGWCC 3 cut(s) 671, 1583, 1766
SlaI CTCGAG 1 cut(s) 1653
SmiMI CAYNNNNRTG 2 cut(s) 177, 585
SmlI CTYRAG 3 cut(s) 65, 1531, 1653
SmoI CTYRAG 3 cut(s) 65, 1531, 1653
SpeI ACTAGT 1 cut(s) 1304
SsiI CCGC 8 cut(s) 51, 105, 666, 1104, 1326, 1509, 1820, 2060
StyD4I CCNGG 5 cut(s) 86, 360, 1144, 1585, 2082
StyI CCWWGG 3 cut(s) 1107, 1537, 1785
TaaI ACNGT 5 cut(s) 499, 625, 1444, 1522, 1864
TaiI ACGT 1 cut(s) 469
TaqI TCGA 4 cut(s) 321, 1463, 1545, 1654
TatI WGTACW 1 cut(s) 982
TauI GCSGC 1 cut(s) 1328
TfiI GAWTC 7 cut(s) 188, 203, 950, 1043, 1460, 1542, 1778
Tru1I TTAA 9 cut(s) 249, 374, 543, 627, 822, 1266, 1487, 1824, 1989
Tru9I TTAA 9 cut(s) 249, 374, 543, 627, 822, 1266, 1487, 1824, 1989
TseFI GTSAC 3 cut(s) 296, 804, 2077
TseI GCWGC 3 cut(s) 224, 233, 998
Tsp45I GTSAC 3 cut(s) 296, 804, 2077
TspDTI ATGAA 7 cut(s) 569, 603, 756, 818, 1577, 1653, 1820
TspGWI ACGGA 1 cut(s) 1231
Tth111I GACNNNGTC 1 cut(s) 2075
Van91I CCANNNNNTGG 1 cut(s) 965
VpaK11BI GGWCC 3 cut(s) 671, 1583, 1766
XapI RAATTY 8 cut(s) 32, 370, 590, 629, 1093, 1118, 1237, 1729
XbaI TCTAGA 2 cut(s) 1334, 2125
XcmI CCANNNNNNNNNTGG 2 cut(s) 465, 1426
XhoI CTCGAG 1 cut(s) 1653
XmiI GTMKAC 2 cut(s) 321, 888
XmnI GAANNNNTTC 3 cut(s) 755, 1397, 1408
ZrmI AGTACT 1 cut(s) 984
Zsp2I ATGCAT 3 cut(s) 572, 1580, 1681
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.