AT5G08750

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0003198
Physical Location & Seq
Forward (+)
2851814 .. 2854566
2753 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G08750.6

Sequence Viewer

Length: 1092 bp
ATGGGTAAAGAGAATCAGTTGGAGACTACCGATTGTGGAGTCAGTGATCACGGTTCTCCACCTATCAAGAATGAAGAACATGAGGCACCACGTTACAGTTCAGACAAAGACACGGGTTTACCAACTTGCCGTGTATGCCAGTGTGCAGAATCTGATAGAAGAGGAGATGCTGCTTTAGGGTTTTTGGGAATTACTCCTCCGGTTTCAGAACCTCGTAGAAGCAATGCGGGTGAAGAAACCGTTGATCAGAGAAGCTCGGTTGTCAAATCTAGCGGATTCATCGAACTGATAAGTCCTGATGGAGAAGTTTTTGTTTGTGCAAACGACGATATAGAAATGGGAGCGTGGCAGCATCGAGATAAGTTGCTAGAACTTGGATGTTCTTGCAAGAATGATCTCGCTTTGGTACACTATGCTTGTGCATTGAAATGGTTTGTCAACCACGGATCAACTGTTTGTGAGATATGCGGAAAAACCACGGAGAATATAAGAACAGTTGATTTCAACAAAGTGGTTATCGCCTTAAAAGATTACGCAGCGCTAAGAGAAAGAACAGCGGATGGAGATCCAAATCCTGTAGTGGTTAACAACAACACCAGTTCGTCAGGGATTGATCCTGATGCAGTTGCAGCCATTCGAAGGCAACGACTTAGTGAGATATCGTTATGGTTTGGTCCTCATTGTTCAACCAACAACAACAATAGTAGTAATTCCGCTGCTGCAGGGACGGCTTCTTCTCAGGTTACATCTGAACAACCTGTGGGTATAGTAAACTTTGATATCTTGCCTATGGAAAGCCGTGCGACTAAATGGGCGGTGGAAGGTACCGGAATACTACTTGCAACCGGATTACTCACAGTTACTTTGGCTTGGCTCATTGCTCCTCGTGTCGGAAAGAGAACTGCAAAGAGTGGTCTACACATACTTCTAGGTGGTCTCTGTGCTTTAACAGTAGTCATCTTCTTCAGATTCGTTGTGCTGACAAGAATCAGGTACGGACCAGCACGTTACTGGGCAATATTGTTCGTCTTCTGGTTTCTTGTGTTTGGCATTTGGGCTTCACGTTCACACGCTTCTCACTCCTCCACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RINGv

Protein Analysis

363

Amino Acids

39.43

Weight (kDa)

6.15

Isoelectric Point (pI)

40.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 120 - 156 8.7e-07 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 824
AccB1I GGYRCC 2 cut(s) 85, 824
AccI GTMKAC 1 cut(s) 916
AciI CCGC 6 cut(s) 227, 273, 468, 557, 714, 815
AclWI GGATC 3 cut(s) 454, 560, 608
AcuI CTGAAG 1 cut(s) 949
AfaI GTAC 3 cut(s) 408, 826, 995
AfeI AGCGCT 1 cut(s) 540
AfiI CCNNNNNNNGG 2 cut(s) 639, 890
AgsI TTSAA 3 cut(s) 427, 505, 687
AluBI AGCT 1 cut(s) 255
AluI AGCT 1 cut(s) 255
Alw26I GTCTC 2 cut(s) 17, 941
AlwI GGATC 3 cut(s) 454, 560, 608
AlwNI CAGNNNCTG 1 cut(s) 152
Aor51HI AGCGCT 1 cut(s) 540
ApeKI GCWGC 6 cut(s) 170, 349, 536, 629, 716, 719
Asp718I GGTACC 1 cut(s) 824
AspLEI GCGC 1 cut(s) 541
AspS9I GGNCC 2 cut(s) 674, 998
AsuHPI GGTGA 1 cut(s) 242
AsuII TTCGAA 1 cut(s) 637
AvaII GGWCC 2 cut(s) 674, 998
BanI GGYRCC 2 cut(s) 85, 824
BauI CACGAG 1 cut(s) 885
BbsI GAAGAC 1 cut(s) 1021
BbvI GCAGC 6 cut(s) 157, 361, 548, 641, 703, 706
BccI CCATC 2 cut(s) 293, 554
BceAI ACGGC 3 cut(s) 114, 744, 783
BclI TGATCA 2 cut(s) 46, 244
BcoDI GTCTC 2 cut(s) 17, 941
BfaI CTAG 3 cut(s) 270, 368, 929
BfmI CTRYAG 2 cut(s) 576, 720
BfoI RGCGCY 1 cut(s) 542
BisI GCNGC 6 cut(s) 171, 350, 537, 630, 717, 720
BlsI GCNGC 6 cut(s) 172, 351, 538, 631, 718, 721
Bme18I GGWCC 2 cut(s) 674, 998
BmgT120I GGNCC 2 cut(s) 674, 998
BmiI GGNNCC 2 cut(s) 87, 826
BmrI ACTGGG 1 cut(s) 1021
BmsI GCATC 3 cut(s) 157, 361, 610
BmuI ACTGGG 1 cut(s) 1021
BpiI GAAGAC 1 cut(s) 1021
Bpu14I TTCGAA 1 cut(s) 637
BsaBI GATNNNNATC 2 cut(s) 564, 570
BsaI GGTCTC 1 cut(s) 941
BsaJI CCNNGG 2 cut(s) 442, 477
BsaWI WCCGGW 3 cut(s) 199, 827, 845
Bsc4I CCNNNNNNNGG 2 cut(s) 639, 890
Bse1I ACTGG 3 cut(s) 139, 597, 1016
Bse3DI GCAATG 2 cut(s) 229, 876
Bse8I GATNNNNATC 2 cut(s) 564, 570
BseDI CCNNGG 2 cut(s) 442, 477
BseGI GGATG 2 cut(s) 383, 565
BseJI GATNNNNATC 2 cut(s) 564, 570
BseLI CCNNNNNNNGG 2 cut(s) 639, 890
BseMI GCAATG 2 cut(s) 229, 876
BseMII CTCAG 1 cut(s) 752
BseNI ACTGG 3 cut(s) 139, 597, 1016
BseRI GAGGAG 4 cut(s) 177, 186, 873, 1072
BseXI GCAGC 6 cut(s) 157, 361, 548, 641, 703, 706
BsgI GTGCAG 1 cut(s) 165
BshNI GGYRCC 2 cut(s) 85, 824
BsiSI CCGG 3 cut(s) 200, 828, 846
BslFI GGGAC 1 cut(s) 739
BslI CCNNNNNNNGG 2 cut(s) 639, 890
BsmAI GTCTC 2 cut(s) 17, 941
BsmFI GGGAC 1 cut(s) 739
Bso31I GGTCTC 1 cut(s) 941
Bsp119I TTCGAA 1 cut(s) 637
Bsp143I GATC 6 cut(s) 46, 244, 394, 446, 565, 613
BspACI CCGC 6 cut(s) 227, 273, 468, 557, 714, 815
BspCNI CTCAG 1 cut(s) 751
BspLI GGNNCC 2 cut(s) 87, 826
BspMAI CTGCAG 1 cut(s) 724
BspPI GGATC 3 cut(s) 454, 560, 608
BspT104I TTCGAA 1 cut(s) 637
BspT107I GGYRCC 2 cut(s) 85, 824
BspTNI GGTCTC 1 cut(s) 941
BsrDI GCAATG 2 cut(s) 229, 876
BsrI ACTGG 3 cut(s) 139, 597, 1016
BssECI CCNNGG 2 cut(s) 442, 477
BssMI GATC 6 cut(s) 46, 244, 394, 446, 565, 613
BssSI CACGAG 1 cut(s) 885
Bst2BI CACGAG 1 cut(s) 885
Bst4CI ACNGT 7 cut(s) 53, 98, 241, 454, 496, 859, 952
Bst6I CTCTTC 1 cut(s) 154
BstBI TTCGAA 1 cut(s) 637
BstDEI CTNAG 3 cut(s) 542, 650, 738
BstDSI CCRYGG 2 cut(s) 442, 477
BstF5I GGATG 2 cut(s) 383, 565
BstH2I RGCGCY 1 cut(s) 542
BstHHI GCGC 1 cut(s) 541
BstKTI GATC 6 cut(s) 49, 247, 397, 449, 568, 616
BstMAI GTCTC 2 cut(s) 17, 941
BstMBI GATC 6 cut(s) 46, 244, 394, 446, 565, 613
BstMWI GCNNNNNNNGC 3 cut(s) 135, 629, 728
BstSFI CTRYAG 2 cut(s) 576, 720
BstV1I GCAGC 6 cut(s) 157, 361, 548, 641, 703, 706
BstV2I GAAGAC 1 cut(s) 1021
BstX2I RGATCY 1 cut(s) 565
BstYI RGATCY 1 cut(s) 565
BtgI CCRYGG 2 cut(s) 442, 477
BtsCI GGATG 2 cut(s) 383, 565
BtsIMutI CAGTG 2 cut(s) 49, 146
CaiI CAGNNNCTG 1 cut(s) 152
CfoI GCGC 1 cut(s) 541
Cfr13I GGNCC 2 cut(s) 674, 998
Csp6I GTAC 3 cut(s) 407, 825, 994
CviAII CATG 2 cut(s) 80, 1089
CviJI RGCY 7 cut(s) 255, 632, 731, 798, 869, 874, 1058
CviKI_1 RGCY 7 cut(s) 255, 632, 731, 798, 869, 874, 1058
CviQI GTAC 3 cut(s) 407, 825, 994
DdeI CTNAG 3 cut(s) 542, 650, 738
DpnI GATC 6 cut(s) 48, 246, 396, 448, 567, 615
DpnII GATC 6 cut(s) 46, 244, 394, 446, 565, 613
Eam1104I CTCTTC 1 cut(s) 154
EarI CTCTTC 1 cut(s) 154
Eco31I GGTCTC 1 cut(s) 941
Eco32I GATATC 2 cut(s) 660, 781
Eco47I GGWCC 2 cut(s) 674, 998
Eco47III AGCGCT 1 cut(s) 540
Eco57I CTGAAG 1 cut(s) 949
EcoRV GATATC 2 cut(s) 660, 781
FaeI CATG 2 cut(s) 83, 1092
FaqI GGGAC 1 cut(s) 739
FatI CATG 2 cut(s) 79, 1088
FauI CCCGC 1 cut(s) 220
FbaI TGATCA 2 cut(s) 46, 244
FblI GTMKAC 1 cut(s) 916
Fnu4HI GCNGC 6 cut(s) 171, 350, 537, 630, 717, 720
FokI GGATG 2 cut(s) 390, 572
Fsp4HI GCNGC 6 cut(s) 171, 350, 537, 630, 717, 720
FspBI CTAG 3 cut(s) 270, 368, 929
GlaI GCGC 1 cut(s) 540
GluI GCNGC 6 cut(s) 171, 350, 537, 630, 717, 720
HaeII RGCGCY 1 cut(s) 542
HapII CCGG 3 cut(s) 200, 828, 846
HhaI GCGC 1 cut(s) 541
Hin1II CATG 2 cut(s) 83, 1092
Hin6I GCGC 1 cut(s) 539
HinP1I GCGC 1 cut(s) 539
HincII GTYRAC 2 cut(s) 439, 586
HindII GTYRAC 2 cut(s) 439, 586
HinfI GANTC 6 cut(s) 13, 39, 149, 276, 969, 987
HpaI GTTAAC 1 cut(s) 586
HpaII CCGG 3 cut(s) 200, 828, 846
HphI GGTGA 1 cut(s) 242
Hpy166II GTNNAC 7 cut(s) 119, 409, 439, 586, 772, 917, 1067
Hpy188I TCNGA 7 cut(s) 103, 154, 208, 249, 751, 893, 968
Hpy188III TCNNGA 4 cut(s) 67, 296, 356, 617
Hpy8I GTNNAC 7 cut(s) 119, 409, 439, 586, 772, 917, 1067
Hpy99I CGWCG 1 cut(s) 329
HpyAV CCTTC 2 cut(s) 633, 815
HpyCH4III ACNGT 7 cut(s) 53, 98, 241, 454, 496, 859, 952
HpyCH4IV ACGT 3 cut(s) 91, 1006, 1063
HpyCH4V TGCA 9 cut(s) 146, 320, 387, 422, 623, 629, 722, 842, 905
HpyF10VI GCNNNNNNNGC 3 cut(s) 135, 629, 728
HpyF3I CTNAG 3 cut(s) 542, 650, 738
HpySE526I ACGT 3 cut(s) 91, 1006, 1063
Hsp92II CATG 2 cut(s) 83, 1092
HspAI GCGC 1 cut(s) 539
KpnI GGTACC 1 cut(s) 828
Ksp22I TGATCA 2 cut(s) 46, 244
KspAI GTTAAC 1 cut(s) 586
Kzo9I GATC 6 cut(s) 46, 244, 394, 446, 565, 613
LmnI GCTCC 2 cut(s) 341, 886
Lsp1109I GCAGC 6 cut(s) 157, 361, 548, 641, 703, 706
LweI GCATC 3 cut(s) 157, 361, 610
MaeI CTAG 3 cut(s) 270, 368, 929
MaeII ACGT 3 cut(s) 91, 1006, 1063
MaeIII GTNAC 4 cut(s) 92, 742, 859, 1007
MalI GATC 6 cut(s) 48, 246, 396, 448, 567, 615
MboI GATC 6 cut(s) 46, 244, 394, 446, 565, 613
MboII GAAGA 7 cut(s) 86, 171, 245, 726, 952, 955, 1021
MflI RGATCY 1 cut(s) 565
MluCI AATT 2 cut(s) 189, 709
MlyI GAGTC 1 cut(s) 48
MmeI TCCRAC 1 cut(s) 871
MnlI CCTC 6 cut(s) 76, 155, 207, 222, 687, 894
MseI TTAA 3 cut(s) 524, 585, 947
MslI CAYNNNNRTG 1 cut(s) 427
MspA1I CMGCKG 2 cut(s) 557, 716
MspI CCGG 3 cut(s) 200, 828, 846
MwoI GCNNNNNNNGC 3 cut(s) 135, 629, 728
NdeII GATC 6 cut(s) 46, 244, 394, 446, 565, 613
NlaIII CATG 2 cut(s) 83, 1092
NlaIV GGNNCC 2 cut(s) 87, 826
NspV TTCGAA 1 cut(s) 637
PcsI WCGNNNNNNNCGW 1 cut(s) 643
PfeI GAWTC 5 cut(s) 13, 149, 276, 969, 987
PkrI GCNGC 6 cut(s) 172, 351, 538, 631, 718, 721
PleI GAGTC 1 cut(s) 47
PpsI GAGTC 1 cut(s) 47
PspN4I GGNNCC 2 cut(s) 87, 826
PspPI GGNCC 2 cut(s) 674, 998
PstI CTGCAG 1 cut(s) 724
PstNI CAGNNNCTG 1 cut(s) 152
PsuI RGATCY 1 cut(s) 565
RsaI GTAC 3 cut(s) 408, 826, 995
RsaNI GTAC 3 cut(s) 407, 825, 994
RseI CAYNNNNRTG 1 cut(s) 427
SaqAI TTAA 3 cut(s) 524, 585, 947
SatI GCNGC 6 cut(s) 171, 350, 537, 630, 717, 720
Sau3AI GATC 6 cut(s) 46, 244, 394, 446, 565, 613
Sau96I GGNCC 2 cut(s) 674, 998
SchI GAGTC 1 cut(s) 48
SfaNI GCATC 3 cut(s) 157, 361, 610
SfcI CTRYAG 2 cut(s) 576, 720
SfuI TTCGAA 1 cut(s) 637
SinI GGWCC 2 cut(s) 674, 998
SmiMI CAYNNNNRTG 1 cut(s) 427
Sse9I AATT 2 cut(s) 189, 709
SsiI CCGC 6 cut(s) 227, 273, 468, 557, 714, 815
SspI AATATT 1 cut(s) 1020
SspMI CTAG 3 cut(s) 270, 368, 929
TaaI ACNGT 7 cut(s) 53, 98, 241, 454, 496, 859, 952
TaiI ACGT 3 cut(s) 94, 1009, 1066
TaqI TCGA 3 cut(s) 282, 355, 637
TasI AATT 2 cut(s) 189, 709
TfiI GAWTC 5 cut(s) 13, 149, 276, 969, 987
Tru1I TTAA 3 cut(s) 524, 585, 947
Tru9I TTAA 3 cut(s) 524, 585, 947
TscAI CASTG 2 cut(s) 49, 146
TseI GCWGC 6 cut(s) 170, 349, 536, 629, 716, 719
TspDTI ATGAA 2 cut(s) 87, 268
TspGWI ACGGA 3 cut(s) 459, 494, 1011
TspRI CASTG 2 cut(s) 49, 146
VpaK11BI GGWCC 2 cut(s) 674, 998
XcmI CCANNNNNNNNNTGG 1 cut(s) 1008
XmiI GTMKAC 1 cut(s) 916
XspI CTAG 3 cut(s) 270, 368, 929
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.