AT5G10150

Protein UPSTREAM OF

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0011027
Physical Location & Seq
Reverse (-)
3181938 .. 3184110
2173 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G10150.1

Sequence Viewer

Length: 1245 bp
ATGGAAGCTGTAAGATGCAGAAGAGGCAGAGAGAACAACAAAAGTCCAGAGAGGATCATAAGGTCTTTGAATCATCATCAACATGATGAAGAACTTGAAGAAGAGGTCAAGACAAAGAAACCCATTTTCCGAAGAGTCCAAGTTGTTTACTATCTCACAAGAAATGGCCACCTTGAACATCCTCACTTCATCGAAGTCATTTCTCCGGTTAACCAGCCTCTCCGGTTAAGAGATGTGATGAACCGGCTTACGATTCTGAGAGGGAAGTGCATGACATCACAATATGCTTGGTCGTGTAAAAGGAGCTATAGAAATGGATTTGTGTGGAATGACTTAGCAGAGAATGATGTGATTTACCCTTCTGACTGTGCTGAATATGTCTTGAAAGGATCTGAAATCACCGACAAATTTCAAGAAGTACATGTGAACAGACCATTGTCTGGTTCTATTCAAGAAGCTCCAAAAAGTCGACTTCTCAGGTCAAAACTCAAGCCTCAAAACCGAACGGCGTCGTTTGACGACGCAGAGCTCTACGTCGGAGAAGAGGAAGAGGAAGAAGATGGAGAGTACGAGCTTTACGAAGAGAAAACGAGTTACACATCTTCCACGACGCCTCAATCTCGCTGTTCTCGAGGCGTATCGACGGAGACAATGGAATCCACCGAACAAAAACCGAACTTGACCAAGACAGAGCAAGATTTGCAAGTACGTTCTGACTCGTCCGATTTGACTCGTTCCAACCCGGTGGTGAAGCCACGTCGACACGAAGTTTCGACGCGGGTCGAGGACGGAGATCCGGTGGAACCCGGATCGGGTCGTGGGTCGATGTGGCTGCAGATGATCTCTTGCGGTCACATCGCCACCAAGTACTACGCACCGAGTGTGATGAATCCGAGACAAAAAGAAGAGAACCTTCGAAAAGGAGTTTTGTGCAAGAACATTGTCAAGAAGACTGTCGTAGATGACGAGCGTGAGATGATAAGGTTCATGTCGGAGAATCCAAGGTTCGGGAATCCTCAGGCGGAGGAGAAAGAGTATTTCAGCGGAAGCATCGTTGAGTCGGTGAGTCAGGAACGAGTCACGGCTGAACCATCGCTAAGACGATCCAACTCGTTCAACGAGGAAAGATCAAAGATTGTTGAGATGGCTAAGGAAACGAAGAAGAAAGAAGAAAGATCGATGGCGAAGGTGAAATGCATACCAAGAACGTGTTTAATGTCATCATCGAAGCAAATCAAGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF966

Protein Analysis

414

Amino Acids

47.95

Weight (kDa)

8.43

Isoelectric Point (pI)

66.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SOK PF06136 46 - 133 5.8e-46 SOSEKI protein DIX-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011027)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10150 AT5G10150 AT5G10150 AT5G10150
fragaria_vesca FvH4_1g21340 FvH4_1g21340
malus_domestica MD01G1025500.v1.1
prunus_persica Prupe.6G181200_v2.0.a1
pyrus_communis pycom01g05360 pycom01g05370
rosa_chinensis RchiOBHm_Chr2g0115201
rosa_laevigata RLG00000018165
rosa_multiflora Rmu_sc0002784.1_g000027
rosa_roxburghii Rroxscaffold_2G00130670
rosa_rugosa Rorug02G0194300
rosa_samantha Rh2AG252800 Rh2BG262400 Rh2CG257400 Rh2DG261000
rosa_wichuraiana Rw2G019720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 440
AccI GTMKAC 2 cut(s) 469, 760
AccII CGCG 1 cut(s) 778
AciI CCGC 4 cut(s) 778, 849, 1022, 1044
AclWI GGATC 5 cut(s) 62, 397, 788, 817, 1098
AcoI YGGCCR 1 cut(s) 166
AcsI RAATTY 1 cut(s) 407
AcyI GRCGYC 2 cut(s) 509, 611
AdeI CACNNNGTG 1 cut(s) 881
AfaI GTAC 4 cut(s) 420, 569, 708, 869
AfiI CCNNNNNNNGG 3 cut(s) 440, 812, 1007
AflIII ACRYGT 2 cut(s) 421, 1208
AgsI TTSAA 7 cut(s) 70, 98, 176, 385, 413, 452, 1117
AjiI CACGTC 1 cut(s) 758
AluBI AGCT 5 cut(s) 8, 306, 458, 529, 574
AluI AGCT 5 cut(s) 8, 306, 458, 529, 574
Alw21I GWGCWC 1 cut(s) 531
Alw26I GTCTC 2 cut(s) 641, 889
AlwI GGATC 5 cut(s) 62, 397, 788, 817, 1098
Ama87I CYCGRG 1 cut(s) 630
AoxI GGCC 1 cut(s) 166
ApeKI GCWGC 1 cut(s) 832
ApoI RAATTY 1 cut(s) 407
AsuC2I CCSGG 2 cut(s) 743, 807
AsuHPI GGTGA 4 cut(s) 391, 760, 1075, 1201
AsuII TTCGAA 1 cut(s) 916
AvaI CYCGRG 1 cut(s) 630
AxyI CCTNAGG 1 cut(s) 1017
BalI TGGCCA 1 cut(s) 168
BanII GRGCYC 1 cut(s) 531
BbsI GAAGAC 1 cut(s) 956
Bbv12I GWGCWC 1 cut(s) 531
BbvI GCAGC 1 cut(s) 819
BccI CCATC 4 cut(s) 554, 1099, 1138, 1174
BceAI ACGGC 2 cut(s) 522, 1098
BcgI CGANNNNNNTGC 2 cut(s) 814, 848
BcnI CCSGG 2 cut(s) 743, 807
BcoDI GTCTC 2 cut(s) 641, 889
BfmI CTRYAG 2 cut(s) 307, 833
BisI GCNGC 1 cut(s) 833
BlsI GCNGC 1 cut(s) 834
BmcAI AGTACT 1 cut(s) 869
Bme1390I CCNGG 2 cut(s) 743, 807
BmeT110I CYCGRG 1 cut(s) 630
BmgBI CACGTC 1 cut(s) 758
BmiI GGNNCC 1 cut(s) 804
BmrFI CCNGG 2 cut(s) 743, 807
BmsI GCATC 2 cut(s) 5, 1059
BoxI GACNNNNGTC 2 cut(s) 436, 779
BpiI GAAGAC 1 cut(s) 956
Bpu10I CCTNAGC 1 cut(s) 1149
Bpu14I TTCGAA 1 cut(s) 916
BpuEI CTTGAG 1 cut(s) 473
BpuMI CCSGG 2 cut(s) 743, 807
Bsa29I ATCGAT 1 cut(s) 1178
BsaHI GRCGYC 2 cut(s) 509, 611
BsaJI CCNNGG 1 cut(s) 1001
BsaWI WCCGGW 3 cut(s) 205, 222, 796
BsaXI ACNNNNNCTCC 4 cut(s) 783, 813, 1019, 1049
Bsc4I CCNNNNNNNGG 3 cut(s) 440, 812, 1007
Bse118I RCCGGY 1 cut(s) 243
Bse21I CCTNAGG 1 cut(s) 1017
BseCI ATCGAT 1 cut(s) 1178
BseDI CCNNGG 1 cut(s) 1001
BseGI GGATG 1 cut(s) 178
BseLI CCNNNNNNNGG 3 cut(s) 440, 812, 1007
BseMII CTCAG 3 cut(s) 248, 490, 1031
BseRI GAGGAG 1 cut(s) 1040
BseXI GCAGC 1 cut(s) 819
Bsh1236I CGCG 1 cut(s) 778
BshFI GGCC 1 cut(s) 168
BshVI ATCGAT 1 cut(s) 1178
BsiHKAI GWGCWC 1 cut(s) 531
BsiHKCI CYCGRG 1 cut(s) 630
BsiSI CCGG 6 cut(s) 206, 223, 244, 743, 797, 807
BslI CCNNNNNNNGG 3 cut(s) 440, 812, 1007
BsmAI GTCTC 2 cut(s) 641, 889
BsnI GGCC 1 cut(s) 168
BsoBI CYCGRG 1 cut(s) 630
Bsp119I TTCGAA 1 cut(s) 916
Bsp1286I GDGCHC 1 cut(s) 531
Bsp143I GATC 8 cut(s) 54, 389, 793, 809, 840, 1103, 1127, 1175
BspACI CCGC 4 cut(s) 778, 849, 1022, 1044
BspANI GGCC 1 cut(s) 168
BspCNI CTCAG 3 cut(s) 249, 489, 1030
BspDI ATCGAT 1 cut(s) 1178
BspFNI CGCG 1 cut(s) 778
BspLI GGNNCC 1 cut(s) 804
BspMAI CTGCAG 1 cut(s) 837
BspPI GGATC 5 cut(s) 62, 397, 788, 817, 1098
BspT104I TTCGAA 1 cut(s) 916
BsrFI RCCGGY 1 cut(s) 243
BssAI RCCGGY 1 cut(s) 243
BssECI CCNNGG 1 cut(s) 1001
BssMI GATC 8 cut(s) 54, 389, 793, 809, 840, 1103, 1127, 1175
BssNI GRCGYC 2 cut(s) 509, 611
BssT1I CCWWGG 1 cut(s) 1001
Bst4CI ACNGT 2 cut(s) 368, 955
Bst6I CTCTTC 7 cut(s) 16, 96, 127, 537, 543, 576, 900
BstACI GRCGYC 2 cut(s) 509, 611
BstAPI GCANNNNNTGC 1 cut(s) 700
BstBI TTCGAA 1 cut(s) 916
BstDEI CTNAG 6 cut(s) 257, 334, 476, 1017, 1097, 1149
BstF5I GGATG 1 cut(s) 178
BstFNI CGCG 1 cut(s) 778
BstKTI GATC 8 cut(s) 57, 392, 796, 812, 843, 1106, 1130, 1178
BstMAI GTCTC 2 cut(s) 641, 889
BstMBI GATC 8 cut(s) 54, 389, 793, 809, 840, 1103, 1127, 1175
BstMWI GCNNNNNNNGC 2 cut(s) 24, 700
BstNSI RCATGY 1 cut(s) 425
BstPAI GACNNNNGTC 2 cut(s) 436, 779
BstSCI CCNGG 2 cut(s) 741, 805
BstSFI CTRYAG 2 cut(s) 307, 833
BstUI CGCG 1 cut(s) 778
BstV1I GCAGC 1 cut(s) 819
BstV2I GAAGAC 1 cut(s) 956
BstX2I RGATCY 2 cut(s) 389, 793
BstXI CCANNNNNNTGG 1 cut(s) 745
BstYI RGATCY 2 cut(s) 389, 793
Bsu15I ATCGAT 1 cut(s) 1178
Bsu36I CCTNAGG 1 cut(s) 1017
BsuRI GGCC 1 cut(s) 168
BsuTUI ATCGAT 1 cut(s) 1178
BtgZI GCGATG 2 cut(s) 841, 1077
BtrI CACGTC 1 cut(s) 758
BtsCI GGATG 1 cut(s) 178
Cfr10I RCCGGY 1 cut(s) 243
ClaI ATCGAT 1 cut(s) 1178
CseI GACGC 4 cut(s) 498, 530, 619, 784
Csp6I GTAC 4 cut(s) 419, 568, 707, 868
CviAII CATG 4 cut(s) 83, 271, 422, 988
CviQI GTAC 4 cut(s) 419, 568, 707, 868
DdeI CTNAG 6 cut(s) 257, 334, 476, 1017, 1097, 1149
DpnI GATC 8 cut(s) 56, 391, 795, 811, 842, 1105, 1129, 1177
DpnII GATC 8 cut(s) 54, 389, 793, 809, 840, 1103, 1127, 1175
DraIII CACNNNGTG 1 cut(s) 881
EaeI YGGCCR 1 cut(s) 166
Eam1104I CTCTTC 7 cut(s) 16, 96, 127, 537, 543, 576, 900
EarI CTCTTC 7 cut(s) 16, 96, 127, 537, 543, 576, 900
EciI GGCGGA 1 cut(s) 1037
Ecl136II GAGCTC 1 cut(s) 529
Eco130I CCWWGG 1 cut(s) 1001
Eco24I GRGCYC 1 cut(s) 531
Eco53kI GAGCTC 1 cut(s) 529
Eco81I CCTNAGG 1 cut(s) 1017
Eco88I CYCGRG 1 cut(s) 630
EcoICRI GAGCTC 1 cut(s) 529
EcoT14I CCWWGG 1 cut(s) 1001
EcoT22I ATGCAT 1 cut(s) 1199
EcoT38I GRGCYC 1 cut(s) 531
ErhI CCWWGG 1 cut(s) 1001
FaeI CATG 4 cut(s) 86, 274, 425, 991
FaiI YATR 9 cut(s) 59, 84, 272, 285, 309, 378, 423, 989, 1199
FalI AAGNNNNNCTT 2 cut(s) 897, 929
FatI CATG 4 cut(s) 82, 270, 421, 987
FauI CCCGC 1 cut(s) 771
FblI GTMKAC 2 cut(s) 469, 760
Fnu4HI GCNGC 1 cut(s) 833
FokI GGATG 1 cut(s) 165
FriOI GRGCYC 1 cut(s) 531
Fsp4HI GCNGC 1 cut(s) 833
GluI GCNGC 1 cut(s) 833
HaeIII GGCC 1 cut(s) 168
HapII CCGG 6 cut(s) 206, 223, 244, 743, 797, 807
HgaI GACGC 4 cut(s) 498, 530, 619, 784
Hin1I GRCGYC 2 cut(s) 509, 611
Hin1II CATG 4 cut(s) 86, 274, 425, 991
HincII GTYRAC 3 cut(s) 211, 470, 761
HindII GTYRAC 3 cut(s) 211, 470, 761
HpaI GTTAAC 1 cut(s) 211
HpaII CCGG 6 cut(s) 206, 223, 244, 743, 797, 807
HphI GGTGA 4 cut(s) 391, 760, 1075, 1201
Hpy166II GTNNAC 5 cut(s) 148, 211, 427, 470, 761
Hpy188I TCNGA 9 cut(s) 131, 258, 364, 394, 539, 715, 724, 894, 994
Hpy8I GTNNAC 5 cut(s) 148, 211, 427, 470, 761
Hpy99I CGWCG 7 cut(s) 514, 524, 539, 613, 646, 762, 778
HpyAV CCTTC 3 cut(s) 369, 923, 1180
HpyCH4III ACNGT 2 cut(s) 368, 955
HpyCH4IV ACGT 4 cut(s) 534, 709, 757, 1208
HpyCH4V TGCA 6 cut(s) 18, 270, 703, 835, 933, 1197
HpyF10VI GCNNNNNNNGC 2 cut(s) 24, 700
HpyF3I CTNAG 6 cut(s) 257, 334, 476, 1017, 1097, 1149
HpySE526I ACGT 4 cut(s) 534, 709, 757, 1208
Hsp92I GRCGYC 2 cut(s) 509, 611
Hsp92II CATG 4 cut(s) 86, 274, 425, 991
KspAI GTTAAC 1 cut(s) 211
Kzo9I GATC 8 cut(s) 54, 389, 793, 809, 840, 1103, 1127, 1175
LmnI GCTCC 2 cut(s) 303, 463
Lsp1109I GCAGC 1 cut(s) 819
LweI GCATC 2 cut(s) 5, 1059
MaeII ACGT 4 cut(s) 534, 709, 757, 1208
MaeIII GTNAC 3 cut(s) 593, 851, 1078
MalI GATC 8 cut(s) 56, 391, 795, 811, 842, 1105, 1129, 1177
MboI GATC 8 cut(s) 54, 389, 793, 809, 840, 1103, 1127, 1175
MflI RGATCY 2 cut(s) 389, 793
MhlI GDGCHC 1 cut(s) 531
MlsI TGGCCA 1 cut(s) 168
MluCI AATT 1 cut(s) 407
MluNI TGGCCA 1 cut(s) 168
MlyI GAGTC 6 cut(s) 144, 710, 724, 1067, 1075, 1086
MmeI TCCRAC 4 cut(s) 517, 762, 972, 1131
Mox20I TGGCCA 1 cut(s) 168
Mph1103I ATGCAT 1 cut(s) 1199
MscI TGGCCA 1 cut(s) 168
MseI TTAA 3 cut(s) 210, 227, 1214
MslI CAYNNNNRTG 1 cut(s) 81
Msp20I TGGCCA 1 cut(s) 168
MspA1I CMGCKG 1 cut(s) 1044
MspI CCGG 6 cut(s) 206, 223, 244, 743, 797, 807
MspR9I CCNGG 2 cut(s) 743, 807
MvnI CGCG 1 cut(s) 778
MwoI GCNNNNNNNGC 2 cut(s) 24, 700
NciI CCSGG 2 cut(s) 743, 807
NdeII GATC 8 cut(s) 54, 389, 793, 809, 840, 1103, 1127, 1175
NlaIII CATG 4 cut(s) 86, 274, 425, 991
NlaIV GGNNCC 1 cut(s) 804
NmuCI GTSAC 2 cut(s) 851, 1078
NsiI ATGCAT 1 cut(s) 1199
NspI RCATGY 1 cut(s) 425
NspV TTCGAA 1 cut(s) 916
PaeR7I CTCGAG 1 cut(s) 630
PciI ACATGT 1 cut(s) 421
PcsI WCGNNNNNNNCGW 3 cut(s) 576, 628, 963
PfeI GAWTC 6 cut(s) 70, 253, 656, 889, 997, 1012
PflMI CCANNNNNTGG 1 cut(s) 440
PkrI GCNGC 1 cut(s) 834
PleI GAGTC 6 cut(s) 143, 710, 724, 1066, 1074, 1085
PpsI GAGTC 6 cut(s) 143, 710, 724, 1066, 1074, 1085
PscI ACATGT 1 cut(s) 421
PshAI GACNNNNGTC 2 cut(s) 436, 779
Psp124BI GAGCTC 1 cut(s) 531
PspN4I GGNNCC 1 cut(s) 804
PstI CTGCAG 1 cut(s) 837
PsuI RGATCY 2 cut(s) 389, 793
RsaI GTAC 4 cut(s) 420, 569, 708, 869
RsaNI GTAC 4 cut(s) 419, 568, 707, 868
RseI CAYNNNNRTG 1 cut(s) 81
SacI GAGCTC 1 cut(s) 531
SalI GTCGAC 2 cut(s) 468, 759
SaqAI TTAA 3 cut(s) 210, 227, 1214
SatI GCNGC 1 cut(s) 833
Sau3AI GATC 8 cut(s) 54, 389, 793, 809, 840, 1103, 1127, 1175
ScaI AGTACT 1 cut(s) 869
SchI GAGTC 6 cut(s) 144, 710, 724, 1067, 1075, 1086
ScrFI CCNGG 2 cut(s) 743, 807
SduI GDGCHC 1 cut(s) 531
SfaNI GCATC 2 cut(s) 5, 1059
SfcI CTRYAG 2 cut(s) 307, 833
Sfr274I CTCGAG 1 cut(s) 630
SfuI TTCGAA 1 cut(s) 916
SlaI CTCGAG 1 cut(s) 630
SmiMI CAYNNNNRTG 1 cut(s) 81
SmlI CTYRAG 2 cut(s) 488, 630
SmoI CTYRAG 2 cut(s) 488, 630
Sse9I AATT 1 cut(s) 407
SsiI CCGC 4 cut(s) 778, 849, 1022, 1044
SstI GAGCTC 1 cut(s) 531
StyD4I CCNGG 2 cut(s) 741, 805
StyI CCWWGG 1 cut(s) 1001
TaaI ACNGT 2 cut(s) 368, 955
TaiI ACGT 4 cut(s) 537, 712, 760, 1211
TasI AATT 1 cut(s) 407
TatI WGTACW 2 cut(s) 418, 867
TfiI GAWTC 6 cut(s) 70, 253, 656, 889, 997, 1012
Tru1I TTAA 3 cut(s) 210, 227, 1214
Tru9I TTAA 3 cut(s) 210, 227, 1214
TseFI GTSAC 2 cut(s) 851, 1078
TseI GCWGC 1 cut(s) 832
Tsp45I GTSAC 2 cut(s) 851, 1078
TspDTI ATGAA 5 cut(s) 102, 178, 254, 902, 976
TspGWI ACGGA 2 cut(s) 659, 804
Van91I CCANNNNNTGG 1 cut(s) 440
XapI RAATTY 1 cut(s) 407
XceI RCATGY 1 cut(s) 425
XhoI CTCGAG 1 cut(s) 630
XmiI GTMKAC 2 cut(s) 469, 760
ZrmI AGTACT 1 cut(s) 869
Zsp2I ATGCAT 1 cut(s) 1199
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.