Rh2DG261000

Protein UPSTREAM OF

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0011027
Physical Location & Seq
Reverse (-)
27998314 .. 28001187
2874 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG261000.1

Sequence Viewer

Length: 1203 bp
ATGGATGTTCGATTTCGAAGACCCCAAACCAGTCCAGATAGAGCCAAAGTGTGCATGCAGCAACTGCAGCCAAGAGTAGCAGTCAACAAACCCACCATCAAAAAGGTCCAAATTGTCTACTACCTCTCAAGAAATGGTCATCTTGAGCATCCTCACTACATGGAGATCACCCATTTGCCCAATCAACCTCTTCGTTTGAGAGATGTAATGGATAGGCTCACTGCTCTCAGAGGCAAGGGCATGCCCTCACTATATTCCTGGTCCTGCAAAAGGAGCTACAAGAACGGTTATGTGTGGAATGATTTAGCAGAAAACGACATAATCTACCCAGCAGACGGAGCTGAGTATGTACTTAAAGGCTCTGAGCTAGTTGAAGGCTGCTCTGAGAGATTGAACCAGATTCAAATAAGCAGCCCCCAACAAATTCAAGACTCAAATTTTGGGTCCAAGAGGAGATCACCACCACTTGCTCCAACCCCAAACAGTAACCACAAGTTTCATGAAGACCATGATCAATATGATGAACAAGAAGAAGAAGAAGTTGACTATGAGGACGAGAAAACAAGCTACACAAGCTCCACAACAACAAACTCTCGCTGCTCCAGAGGAGTCTCCACAGATGAACTCGAAGAACAACAACAACCACCGCAAACCCACAAGACTCCCCCCGAGTCATCTCCTCTCCCAAATGCACCAGCTTCAGTGTTGAACCAACAAAACCACAGCAACAATGTGTCCATGAGGTTCGAGGACGGCGACCCCATTGTTACGAAACAGAGCTACTCTGTTTCGAAGGCAGGGCAGGGTAAGAACTCTGTTCTGCTCCAGCTCATTGCTTGCGGCAGCTCGGCCGTGGCTAAGGCCAAAAGCACACCGTGCGTGAGTCAAGGGATTGTGAAGAGGGAGAGGAGTAGTCAGAGTTTTCGTAAAGAGGTGGTGTACAGGAGTGCGGTGAAGGTTGCCATAGAGGATGAGGATATGATTACTTACATGTCTGAGAATCCGAGGTTTGGGAATTTACAGGCGGAGGAGAAGGAGTATTTCAGTGGGAGCATTGTGGAGTCCATGAAGGAAGATCAAAGAGTACATTCAGCTGAGCCAGTGCTCAAAAAATCCAATTCCTATAATGAAGAAAGGTCAGTTTTCATGTTCTTCATGCAATATGCATATTTAGACCTTATTTGCATAGTTTGTAATGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF966

Protein Analysis

400

Amino Acids

45.52

Weight (kDa)

6.11

Isoelectric Point (pI)

63.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SOK PF06136 36 - 124 8.8e-48 SOSEKI protein DIX-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011027)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10150 AT5G10150 AT5G10150 AT5G10150
fragaria_vesca FvH4_1g21340 FvH4_1g21340
malus_domestica MD01G1025500.v1.1
prunus_persica Prupe.6G181200_v2.0.a1
pyrus_communis pycom01g05360 pycom01g05370
rosa_chinensis RchiOBHm_Chr2g0115201
rosa_laevigata RLG00000018165
rosa_multiflora Rmu_sc0002784.1_g000027
rosa_roxburghii Rroxscaffold_2G00130670
rosa_rugosa Rorug02G0194300
rosa_samantha Rh2AG252800 Rh2BG262400 Rh2CG257400 Rh2DG261000
rosa_wichuraiana Rw2G019720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 117
AciI CCGC 4 cut(s) 647, 840, 950, 1025
AcoI YGGCCR 1 cut(s) 849
AcsI RAATTY 3 cut(s) 423, 436, 1015
AcuI CTGAAG 1 cut(s) 684
AdeI CACNNNGTG 1 cut(s) 876
AfaI GTAC 3 cut(s) 351, 941, 1086
AfiI CCNNNNNNNGG 3 cut(s) 270, 335, 1010
AflIII ACRYGT 1 cut(s) 990
AgsI TTSAA 5 cut(s) 374, 394, 404, 428, 709
AjnI CCWGG 1 cut(s) 257
Alw21I GWGCWC 1 cut(s) 1107
Alw26I GTCTC 1 cut(s) 616
AlwNI CAGNNNCTG 1 cut(s) 64
Ama87I CYCGRG 1 cut(s) 668
AoxI GGCC 2 cut(s) 849, 861
ApeKI GCWGC 6 cut(s) 58, 67, 378, 411, 597, 843
ApoI RAATTY 3 cut(s) 423, 436, 1015
ArsI GACNNNNNNTTYG 4 cut(s) 422, 428, 454, 460
AspS9I GGNCC 3 cut(s) 106, 261, 444
AsuHPI GGTGA 3 cut(s) 160, 450, 964
AsuII TTCGAA 2 cut(s) 16, 791
AvaI CYCGRG 1 cut(s) 668
AvaII GGWCC 3 cut(s) 106, 261, 444
BbsI GAAGAC 2 cut(s) 25, 510
Bbv12I GWGCWC 1 cut(s) 1107
BbvI GCAGC 6 cut(s) 70, 79, 365, 423, 584, 855
BccI CCATC 1 cut(s) 104
BceAI ACGGC 2 cut(s) 769, 836
BciT130I CCWGG 1 cut(s) 259
BclI TGATCA 1 cut(s) 511
BcoDI GTCTC 1 cut(s) 616
BfaI CTAG 1 cut(s) 368
BfmI CTRYAG 1 cut(s) 65
BisI GCNGC 7 cut(s) 59, 68, 379, 412, 598, 841, 844
BlpI GCTNAGC 1 cut(s) 1095
BlsI GCNGC 7 cut(s) 60, 69, 380, 413, 599, 842, 845
Bme1390I CCNGG 1 cut(s) 259
Bme18I GGWCC 3 cut(s) 106, 261, 444
BmeT110I CYCGRG 1 cut(s) 668
BmgT120I GGNCC 3 cut(s) 106, 261, 444
BmiI GGNNCC 1 cut(s) 445
BmrFI CCNGG 1 cut(s) 259
BmsI GCATC 1 cut(s) 157
BpiI GAAGAC 2 cut(s) 25, 510
BpmI CTGGAG 2 cut(s) 586, 809
Bpu10I CCTNAGC 1 cut(s) 858
Bpu1102I GCTNAGC 1 cut(s) 1095
Bpu14I TTCGAA 2 cut(s) 16, 791
BpuEI CTTGAG 2 cut(s) 112, 164
BsaJI CCNNGG 2 cut(s) 852, 1004
BsaXI ACNNNNNCTCC 4 cut(s) 560, 590, 1022, 1052
Bsc4I CCNNNNNNNGG 3 cut(s) 270, 335, 1010
Bse1I ACTGG 2 cut(s) 30, 1100
Bse3DI GCAATG 1 cut(s) 831
BseBI CCWGG 1 cut(s) 259
BseDI CCNNGG 2 cut(s) 852, 1004
BseGI GGATG 3 cut(s) 10, 148, 976
BseLI CCNNNNNNNGG 3 cut(s) 270, 335, 1010
BseMI GCAATG 1 cut(s) 831
BseMII CTCAG 6 cut(s) 241, 333, 354, 375, 987, 1086
BseNI ACTGG 2 cut(s) 30, 1100
BseRI GAGGAG 5 cut(s) 466, 621, 669, 922, 1043
BseX3I CGGCCG 1 cut(s) 849
BseXI GCAGC 6 cut(s) 70, 79, 365, 423, 584, 855
BseYI CCCAGC 1 cut(s) 328
Bsh1285I CGRYCG 1 cut(s) 852
BshFI GGCC 2 cut(s) 851, 863
BsiEI CGRYCG 1 cut(s) 852
BsiHKAI GWGCWC 1 cut(s) 1107
BsiHKCI CYCGRG 1 cut(s) 668
BslI CCNNNNNNNGG 3 cut(s) 270, 335, 1010
BsmAI GTCTC 1 cut(s) 616
BsnI GGCC 2 cut(s) 851, 863
BsoBI CYCGRG 1 cut(s) 668
Bsp119I TTCGAA 2 cut(s) 16, 791
Bsp1286I GDGCHC 1 cut(s) 1107
Bsp1407I TGTACA 1 cut(s) 939
Bsp143I GATC 4 cut(s) 165, 455, 511, 1075
Bsp1720I GCTNAGC 1 cut(s) 1095
BspACI CCGC 4 cut(s) 647, 840, 950, 1025
BspANI GGCC 2 cut(s) 851, 863
BspCNI CTCAG 6 cut(s) 240, 334, 355, 376, 988, 1087
BspHI TCATGA 1 cut(s) 499
BspLI GGNNCC 1 cut(s) 445
BspMAI CTGCAG 1 cut(s) 69
BspT104I TTCGAA 2 cut(s) 16, 791
BsrDI GCAATG 1 cut(s) 831
BsrGI TGTACA 1 cut(s) 939
BsrI ACTGG 2 cut(s) 30, 1100
BssECI CCNNGG 2 cut(s) 852, 1004
BssMI GATC 4 cut(s) 165, 455, 511, 1075
Bst2UI CCWGG 1 cut(s) 259
Bst4CI ACNGT 3 cut(s) 287, 485, 876
Bst6I CTCTTC 2 cut(s) 195, 893
BstAPI GCANNNNNTGC 2 cut(s) 64, 876
BstAUI TGTACA 1 cut(s) 939
BstBI TTCGAA 2 cut(s) 16, 791
BstC8I GCNNGC 3 cut(s) 56, 242, 838
BstDEI CTNAG 7 cut(s) 227, 342, 363, 384, 858, 996, 1095
BstDSI CCRYGG 1 cut(s) 852
BstENI CCTNNNNNAGG 1 cut(s) 268
BstF5I GGATG 3 cut(s) 10, 148, 976
BstKTI GATC 4 cut(s) 168, 458, 514, 1078
BstMAI GTCTC 1 cut(s) 616
BstMBI GATC 4 cut(s) 165, 455, 511, 1075
BstMCI CGRYCG 1 cut(s) 852
BstMWI GCNNNNNNNGC 6 cut(s) 64, 67, 273, 338, 573, 876
BstNI CCWGG 1 cut(s) 259
BstNSI RCATGY 3 cut(s) 58, 244, 994
BstSCI CCNGG 1 cut(s) 257
BstSFI CTRYAG 1 cut(s) 65
BstV1I GCAGC 6 cut(s) 70, 79, 365, 423, 584, 855
BstV2I GAAGAC 2 cut(s) 25, 510
BstZI CGGCCG 1 cut(s) 849
BsuRI GGCC 2 cut(s) 851, 863
BtgI CCRYGG 1 cut(s) 852
BtsCI GGATG 3 cut(s) 10, 148, 976
BtsI GCAGTG 1 cut(s) 219
BtsIMutI CAGTG 4 cut(s) 219, 708, 1051, 1107
Cac8I GCNNGC 3 cut(s) 56, 242, 838
CaiI CAGNNNCTG 1 cut(s) 64
CciI TCATGA 1 cut(s) 499
Cfr13I GGNCC 3 cut(s) 106, 261, 444
Csp6I GTAC 3 cut(s) 350, 940, 1085
CviQI GTAC 3 cut(s) 350, 940, 1085
DdeI CTNAG 7 cut(s) 227, 342, 363, 384, 858, 996, 1095
DpnI GATC 4 cut(s) 167, 457, 513, 1077
DpnII GATC 4 cut(s) 165, 455, 511, 1075
DraIII CACNNNGTG 1 cut(s) 876
EaeI YGGCCR 1 cut(s) 849
EagI CGGCCG 1 cut(s) 849
Eam1104I CTCTTC 2 cut(s) 195, 893
EarI CTCTTC 2 cut(s) 195, 893
EciI GGCGGA 1 cut(s) 1040
EclXI CGGCCG 1 cut(s) 849
Eco47I GGWCC 3 cut(s) 106, 261, 444
Eco52I CGGCCG 1 cut(s) 849
Eco57I CTGAAG 1 cut(s) 684
Eco88I CYCGRG 1 cut(s) 668
EcoNI CCTNNNNNAGG 1 cut(s) 268
EcoRII CCWGG 1 cut(s) 257
EcoT22I ATGCAT 1 cut(s) 1168
FbaI TGATCA 1 cut(s) 511
FblI GTMKAC 1 cut(s) 117
Fnu4HI GCNGC 7 cut(s) 59, 68, 379, 412, 598, 841, 844
FokI GGATG 3 cut(s) 17, 135, 983
Fsp4HI GCNGC 7 cut(s) 59, 68, 379, 412, 598, 841, 844
FspBI CTAG 1 cut(s) 368
GluI GCNGC 7 cut(s) 59, 68, 379, 412, 598, 841, 844
GsaI CCCAGC 1 cut(s) 332
GsuI CTGGAG 2 cut(s) 586, 809
HaeIII GGCC 2 cut(s) 851, 863
HincII GTYRAC 2 cut(s) 85, 544
HindII GTYRAC 2 cut(s) 85, 544
HinfI GANTC 8 cut(s) 400, 431, 609, 661, 671, 883, 1000, 1061
HphI GGTGA 3 cut(s) 160, 450, 964
Hpy166II GTNNAC 4 cut(s) 85, 118, 544, 940
Hpy188I TCNGA 6 cut(s) 230, 364, 385, 918, 997, 1005
Hpy188III TCNNGA 6 cut(s) 35, 129, 143, 428, 500, 603
Hpy8I GTNNAC 4 cut(s) 85, 118, 544, 940
HpyAV CCTTC 5 cut(s) 368, 787, 949, 1027, 1063
HpyCH4III ACNGT 3 cut(s) 287, 485, 876
HpyCH4V TGCA 8 cut(s) 54, 58, 67, 267, 692, 1159, 1166, 1185
HpyF10VI GCNNNNNNNGC 6 cut(s) 64, 67, 273, 338, 573, 876
HpyF3I CTNAG 7 cut(s) 227, 342, 363, 384, 858, 996, 1095
Ksp22I TGATCA 1 cut(s) 511
Kzo9I GATC 4 cut(s) 165, 455, 511, 1075
LmnI GCTCC 7 cut(s) 273, 338, 475, 581, 605, 828, 1050
Lsp1109I GCAGC 6 cut(s) 70, 79, 365, 423, 584, 855
LweI GCATC 1 cut(s) 157
MaeI CTAG 1 cut(s) 368
MaeIII GTNAC 2 cut(s) 485, 766
MalI GATC 4 cut(s) 167, 457, 513, 1077
MboI GATC 4 cut(s) 165, 455, 511, 1075
MhlI GDGCHC 1 cut(s) 1107
MluCI AATT 5 cut(s) 111, 423, 436, 1015, 1117
MlyI GAGTC 6 cut(s) 425, 618, 655, 680, 892, 1070
MmeI TCCRAC 1 cut(s) 497
Mph1103I ATGCAT 1 cut(s) 1168
MseI TTAA 1 cut(s) 354
MspA1I CMGCKG 1 cut(s) 1094
MspR9I CCNGG 1 cut(s) 259
MvaI CCWGG 1 cut(s) 259
MwoI GCNNNNNNNGC 6 cut(s) 64, 67, 273, 338, 573, 876
NdeII GATC 4 cut(s) 165, 455, 511, 1075
NlaIV GGNNCC 1 cut(s) 445
NmeAIII GCCGAG 1 cut(s) 827
NsiI ATGCAT 1 cut(s) 1168
NspI RCATGY 3 cut(s) 58, 244, 994
NspV TTCGAA 2 cut(s) 16, 791
PaeI GCATGC 2 cut(s) 58, 244
PagI TCATGA 1 cut(s) 499
PciI ACATGT 1 cut(s) 990
PcsI WCGNNNNNNNCGW 1 cut(s) 753
PfeI GAWTC 2 cut(s) 400, 1000
PkrI GCNGC 7 cut(s) 60, 69, 380, 413, 599, 842, 845
PleI GAGTC 6 cut(s) 425, 617, 655, 679, 891, 1069
PpsI GAGTC 6 cut(s) 425, 617, 655, 679, 891, 1069
PscI ACATGT 1 cut(s) 990
Psp6I CCWGG 1 cut(s) 257
PspFI CCCAGC 1 cut(s) 328
PspGI CCWGG 1 cut(s) 257
PspN4I GGNNCC 1 cut(s) 445
PspPI GGNCC 3 cut(s) 106, 261, 444
PstI CTGCAG 1 cut(s) 69
PstNI CAGNNNCTG 1 cut(s) 64
PvuII CAGCTG 1 cut(s) 1094
RsaI GTAC 3 cut(s) 351, 941, 1086
RsaNI GTAC 3 cut(s) 350, 940, 1085
SaqAI TTAA 1 cut(s) 354
SatI GCNGC 7 cut(s) 59, 68, 379, 412, 598, 841, 844
Sau3AI GATC 4 cut(s) 165, 455, 511, 1075
Sau96I GGNCC 3 cut(s) 106, 261, 444
SchI GAGTC 6 cut(s) 425, 618, 655, 680, 892, 1070
ScrFI CCNGG 1 cut(s) 259
SduI GDGCHC 1 cut(s) 1107
SfaNI GCATC 1 cut(s) 157
SfcI CTRYAG 1 cut(s) 65
SfuI TTCGAA 2 cut(s) 16, 791
SinI GGWCC 3 cut(s) 106, 261, 444
SmlI CTYRAG 2 cut(s) 127, 143
SmoI CTYRAG 2 cut(s) 127, 143
SphI GCATGC 2 cut(s) 58, 244
Sse9I AATT 5 cut(s) 111, 423, 436, 1015, 1117
SsiI CCGC 4 cut(s) 647, 840, 950, 1025
SspMI CTAG 1 cut(s) 368
StyD4I CCNGG 1 cut(s) 257
TaaI ACNGT 3 cut(s) 287, 485, 876
TaqI TCGA 5 cut(s) 10, 16, 627, 747, 791
TasI AATT 5 cut(s) 111, 423, 436, 1015, 1117
TatI WGTACW 3 cut(s) 349, 939, 1084
TauI GCSGC 1 cut(s) 843
TfiI GAWTC 2 cut(s) 400, 1000
Tru1I TTAA 1 cut(s) 354
Tru9I TTAA 1 cut(s) 354
TscAI CASTG 4 cut(s) 226, 708, 1051, 1107
TseI GCWGC 6 cut(s) 58, 67, 378, 411, 597, 843
TspDTI ATGAA 8 cut(s) 488, 516, 537, 636, 1082, 1135, 1143, 1144
TspGWI ACGGA 1 cut(s) 351
TspRI CASTG 4 cut(s) 226, 708, 1051, 1107
VpaK11BI GGWCC 3 cut(s) 106, 261, 444
XagI CCTNNNNNAGG 1 cut(s) 268
XapI RAATTY 3 cut(s) 423, 436, 1015
XceI RCATGY 3 cut(s) 58, 244, 994
XmiI GTMKAC 1 cut(s) 117
XspI CTAG 1 cut(s) 368
Zsp2I ATGCAT 1 cut(s) 1168
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.