MD01G1025500.v1.1

Protein UPSTREAM OF

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0011027
Physical Location & Seq
Forward (+)
9760235 .. 9762744
2510 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1025500.v1.1.491

Sequence Viewer

Length: 1347 bp
ATGGATGTTCGTTTCAGAAGACCTACCACCACCAGAGAATTGAGAGAAAGCAGCACAGACAGAGCCGAAGTTATGCAGCAGCCACCAAGAGCCATAAAAAAACTTCAAGTTGTCTACTATCTCTCAAGAAATGGCCATCTCGAGCATCCTCATTACATGGAAATCACCCACTTGGCCAATCACCCGCTTCGTTTGAGAGATGTAATGGATCGGCTGACTGCTCTTAGAGGCAAAGGCATGCCCGCCTTGTACTCTTGGTCTTGCAAAAGGAGCTACAAAAGTGGTTATGTGTGGAACGATTTAGCCGAAAACGATATAATTTATCCAGCAGAAGGAGCTGAGTATGTACTCAAGGGATCAGAGCTAGTTGAAGGCTGCTCTGCAGAAAGATTAGATCAACTGCAAATGAGTAGCAACCAACAGATGATGCAGCAGCAAGACTCAAACTTTGGGTCCAAAAGGAGATCTCCACTCGCTCCGACTCGAAGCAGAGGACATGATCATCATCATCGAGAAGCAGACAAGTATCGGGAGGAGGAGGGTCAAGACCAAGACTCTGAGGACGAACAAGAAGAACAAGAACTGGTGGACTATGAGGATGAGAAAACAAGCTACACCAGCTCCACCACCCCCCATTCTCGCTGCTCCAGAGGGGTCTCCACAGACGAACTCGAAGACCCTCCACAAACCCGCAAAAGCCCGACTGTGTCAACTCACCCCGAGTCATCACCGCCTCCTCCTCCAACTCCAACCCCATCAGTTAATAATATAGCATCAAATCAGCCCAGCCAGCGCACCAATCTGTCCACATGGTTCGAGGACGGCGACCCAGTTGTGGTTTCGGATTCCAAGTCCTCAGCACTGCAGAGCAAGAACTCTGTTTTGCTTCAGCTCATAGCTTGCGGGAGCTCAGCAGTATCCAAAGCAAAAAATGCACCATGTGTGAGTCTGAAGCAACACACAACAGCGACAGATCATGTGAAGAAGAGCAGCGAGAGCTTCCGGAAAGAGGTTGTGTATAAGGCTGCAGTGAAGGTGGCGGTGGCCGCGGAGGAGGACATGATTAAATGCATGTCTGAGAATCCAAGGTTTGGGAATTTACAGGCAGAGGAGAAAGAGTATTTTAGTGGGAGTATTGTGGACGCCATGAAAGACCAAGATCGAGTTTTGGCTGAACCTAGGCCTGGGCTTAAAAGATCAAATTCCTACAACGAAGAAAGGAGCACCAAGGCTGGGTTTGTTTCAGAAAGTGTAGAAGAAGAGAAGAAAGAGAAAGCAGCGGTGAAAGGGAAGTGCATTCCTCGTAAACTATATTCTTCTGCTGCATCAAAACAAAGCAAAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF966

Protein Analysis

449

Amino Acids

50.4

Weight (kDa)

6.7

Isoelectric Point (pI)

62.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SOK PF06136 36 - 123 7.7e-47 SOSEKI protein DIX-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011027)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10150 AT5G10150 AT5G10150 AT5G10150
fragaria_vesca FvH4_1g21340 FvH4_1g21340
malus_domestica MD01G1025500.v1.1
prunus_persica Prupe.6G181200_v2.0.a1
pyrus_communis pycom01g05360 pycom01g05370
rosa_chinensis RchiOBHm_Chr2g0115201
rosa_laevigata RLG00000018165
rosa_multiflora Rmu_sc0002784.1_g000027
rosa_roxburghii Rroxscaffold_2G00130670
rosa_rugosa Rorug02G0194300
rosa_samantha Rh2AG252800 Rh2BG262400 Rh2CG257400 Rh2DG261000
rosa_wichuraiana Rw2G019720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1091
AccI GTMKAC 1 cut(s) 114
AccII CGCG 1 cut(s) 1049
AccIII TCCGGA 1 cut(s) 1002
AciI CCGC 9 cut(s) 185, 243, 691, 731, 903, 1040, 1047, 1049, 1280
AclWI GGATC 2 cut(s) 216, 364
AcoI YGGCCR 3 cut(s) 133, 174, 1044
AcsI RAATTY 2 cut(s) 1096, 1201
AcuI CTGAAG 2 cut(s) 872, 971
AcyI GRCGYC 1 cut(s) 1143
AdeI CACNNNGTG 1 cut(s) 941
AfaI GTAC 2 cut(s) 251, 348
AfiI CCNNNNNNNGG 6 cut(s) 332, 835, 1009, 1091, 1184, 1233
AgsI TTSAA 2 cut(s) 107, 371
AjnI CCWGG 1 cut(s) 1183
AluBI AGCT 9 cut(s) 273, 338, 364, 612, 621, 892, 899, 909, 999
AluI AGCT 9 cut(s) 273, 338, 364, 612, 621, 892, 899, 909, 999
Alw21I GWGCWC 2 cut(s) 911, 1226
Alw26I GTCTC 1 cut(s) 661
AlwI GGATC 2 cut(s) 216, 364
Ama87I CYCGRG 2 cut(s) 140, 719
Aor13HI TCCGGA 1 cut(s) 1002
AoxI GGCC 4 cut(s) 133, 174, 1044, 1181
ApoI RAATTY 2 cut(s) 1096, 1201
ArsI GACNNNNNNTTYG 4 cut(s) 431, 437, 463, 469
AspA2I CCTAGG 1 cut(s) 1178
AspLEI GCGC 1 cut(s) 795
AspS9I GGNCC 1 cut(s) 453
AsuHPI GGTGA 5 cut(s) 157, 173, 707, 720, 1294
AvaI CYCGRG 2 cut(s) 140, 719
AvaII GGWCC 1 cut(s) 453
AvrII CCTAGG 1 cut(s) 1178
BalI TGGCCA 2 cut(s) 135, 176
BanII GRGCYC 1 cut(s) 911
BbsI GAAGAC 2 cut(s) 25, 681
Bbv12I GWGCWC 2 cut(s) 911, 1226
BbvCI CCTCAGC 1 cut(s) 856
BccI CCATC 2 cut(s) 144, 763
BceAI ACGGC 1 cut(s) 838
BciT130I CCWGG 1 cut(s) 1185
BciVI GTATCC 1 cut(s) 928
BclI TGATCA 1 cut(s) 499
BcoDI GTCTC 1 cut(s) 661
BfaI CTAG 2 cut(s) 365, 1179
BfmI CTRYAG 3 cut(s) 381, 863, 1026
BfuI GTATCC 1 cut(s) 928
BglII AGATCT 1 cut(s) 464
BlnI CCTAGG 1 cut(s) 1178
BlpI GCTNAGC 1 cut(s) 910
Bme1390I CCNGG 1 cut(s) 1185
Bme18I GGWCC 1 cut(s) 453
BmeT110I CYCGRG 2 cut(s) 140, 719
BmgT120I GGNCC 1 cut(s) 453
BmiI GGNNCC 1 cut(s) 454
BmrFI CCNGG 1 cut(s) 1185
BmrI ACTGGG 1 cut(s) 824
BmsI GCATC 4 cut(s) 154, 417, 782, 1334
BmuI ACTGGG 1 cut(s) 824
BpiI GAAGAC 2 cut(s) 25, 681
BpmI CTGGAG 1 cut(s) 631
Bpu10I CCTNAGC 1 cut(s) 856
Bpu1102I GCTNAGC 1 cut(s) 910
BpuEI CTTGAG 2 cut(s) 109, 335
BsaBI GATNNNNATC 1 cut(s) 504
BsaHI GRCGYC 1 cut(s) 1143
BsaI GGTCTC 1 cut(s) 661
BsaJI CCNNGG 5 cut(s) 1047, 1085, 1178, 1184, 1227
BsaWI WCCGGW 1 cut(s) 1002
BsaXI ACNNNNNCTCC 4 cut(s) 605, 635, 1103, 1133
Bsc4I CCNNNNNNNGG 6 cut(s) 332, 835, 1009, 1091, 1184, 1233
Bse1I ACTGG 2 cut(s) 588, 830
Bse8I GATNNNNATC 1 cut(s) 504
BseAI TCCGGA 1 cut(s) 1002
BseBI CCWGG 1 cut(s) 1185
BseDI CCNNGG 5 cut(s) 1047, 1085, 1178, 1184, 1227
BseGI GGATG 3 cut(s) 10, 145, 604
BseJI GATNNNNATC 1 cut(s) 504
BseLI CCNNNNNNNGG 6 cut(s) 332, 835, 1009, 1091, 1184, 1233
BseMII CTCAG 5 cut(s) 330, 549, 870, 924, 1068
BseNI ACTGG 2 cut(s) 588, 830
BseRI GAGGAG 6 cut(s) 548, 551, 726, 729, 1067, 1124
BseYI CCCAGC 2 cut(s) 785, 1232
Bsh1236I CGCG 1 cut(s) 1049
BshFI GGCC 4 cut(s) 135, 176, 1046, 1183
BsiHKAI GWGCWC 2 cut(s) 911, 1226
BsiHKCI CYCGRG 2 cut(s) 140, 719
BsiSI CCGG 1 cut(s) 1003
BslI CCNNNNNNNGG 6 cut(s) 332, 835, 1009, 1091, 1184, 1233
BsmAI GTCTC 1 cut(s) 661
BsmI GAATGC 1 cut(s) 1296
BsnI GGCC 4 cut(s) 135, 176, 1046, 1183
Bso31I GGTCTC 1 cut(s) 661
BsoBI CYCGRG 2 cut(s) 140, 719
Bsp1286I GDGCHC 2 cut(s) 911, 1226
Bsp13I TCCGGA 1 cut(s) 1002
Bsp143I GATC 8 cut(s) 208, 356, 394, 464, 499, 973, 1159, 1196
Bsp1720I GCTNAGC 1 cut(s) 910
BspACI CCGC 9 cut(s) 185, 243, 691, 731, 903, 1040, 1047, 1049, 1280
BspANI GGCC 4 cut(s) 135, 176, 1046, 1183
BspCNI CTCAG 5 cut(s) 331, 550, 869, 923, 1069
BspEI TCCGGA 1 cut(s) 1002
BspFNI CGCG 1 cut(s) 1049
BspLI GGNNCC 1 cut(s) 454
BspMAI CTGCAG 3 cut(s) 385, 867, 1030
BspPI GGATC 2 cut(s) 216, 364
BspQI GCTCTTC 1 cut(s) 980
BspTNI GGTCTC 1 cut(s) 661
BsrI ACTGG 2 cut(s) 588, 830
BssECI CCNNGG 5 cut(s) 1047, 1085, 1178, 1184, 1227
BssMI GATC 8 cut(s) 208, 356, 394, 464, 499, 973, 1159, 1196
BssNI GRCGYC 1 cut(s) 1143
BssT1I CCWWGG 3 cut(s) 1085, 1178, 1227
Bst2UI CCWGG 1 cut(s) 1185
Bst4CI ACNGT 1 cut(s) 706
Bst6I CTCTTC 2 cut(s) 980, 1254
BstACI GRCGYC 1 cut(s) 1143
BstAPI GCANNNNNTGC 1 cut(s) 932
BstC8I GCNNGC 4 cut(s) 239, 243, 791, 901
BstDEI CTNAG 6 cut(s) 224, 339, 558, 856, 910, 1077
BstDSI CCRYGG 1 cut(s) 1047
BstF5I GGATG 3 cut(s) 10, 145, 604
BstFNI CGCG 1 cut(s) 1049
BstHHI GCGC 1 cut(s) 795
BstKTI GATC 8 cut(s) 211, 359, 397, 467, 502, 976, 1162, 1199
BstMAI GTCTC 1 cut(s) 661
BstMBI GATC 8 cut(s) 208, 356, 394, 464, 499, 973, 1159, 1196
BstMWI GCNNNNNNNGC 7 cut(s) 270, 335, 618, 790, 932, 996, 1046
BstNI CCWGG 1 cut(s) 1185
BstNSI RCATGY 2 cut(s) 241, 1075
BstSCI CCNGG 1 cut(s) 1183
BstSFI CTRYAG 3 cut(s) 381, 863, 1026
BstUI CGCG 1 cut(s) 1049
BstV2I GAAGAC 2 cut(s) 25, 681
BstX2I RGATCY 1 cut(s) 464
BstYI RGATCY 1 cut(s) 464
BsuI GTATCC 1 cut(s) 928
BsuRI GGCC 4 cut(s) 135, 176, 1046, 1183
BtgI CCRYGG 1 cut(s) 1047
BtsCI GGATG 3 cut(s) 10, 145, 604
BtsI GCAGTG 2 cut(s) 860, 1035
BtsIMutI CAGTG 2 cut(s) 860, 1035
Cac8I GCNNGC 4 cut(s) 239, 243, 791, 901
CfoI GCGC 1 cut(s) 795
Cfr13I GGNCC 1 cut(s) 453
Cfr42I CCGCGG 1 cut(s) 1050
CseI GACGC 1 cut(s) 1151
Csp6I GTAC 2 cut(s) 250, 347
CviAII CATG 9 cut(s) 157, 238, 497, 810, 939, 977, 1060, 1072, 1147
CviQI GTAC 2 cut(s) 250, 347
DdeI CTNAG 6 cut(s) 224, 339, 558, 856, 910, 1077
DpnI GATC 8 cut(s) 210, 358, 396, 466, 501, 975, 1161, 1198
DpnII GATC 8 cut(s) 208, 356, 394, 464, 499, 973, 1159, 1196
DraIII CACNNNGTG 1 cut(s) 941
EaeI YGGCCR 3 cut(s) 133, 174, 1044
Eam1104I CTCTTC 2 cut(s) 980, 1254
EarI CTCTTC 2 cut(s) 980, 1254
Ecl136II GAGCTC 1 cut(s) 909
Eco130I CCWWGG 3 cut(s) 1085, 1178, 1227
Eco147I AGGCCT 1 cut(s) 1183
Eco24I GRGCYC 1 cut(s) 911
Eco31I GGTCTC 1 cut(s) 661
Eco47I GGWCC 1 cut(s) 453
Eco53kI GAGCTC 1 cut(s) 909
Eco57I CTGAAG 2 cut(s) 872, 971
Eco88I CYCGRG 2 cut(s) 140, 719
EcoICRI GAGCTC 1 cut(s) 909
EcoRII CCWGG 1 cut(s) 1183
EcoT14I CCWWGG 3 cut(s) 1085, 1178, 1227
EcoT22I ATGCAT 1 cut(s) 1073
EcoT38I GRGCYC 1 cut(s) 911
ErhI CCWWGG 3 cut(s) 1085, 1178, 1227
FaeI CATG 9 cut(s) 160, 241, 500, 813, 942, 980, 1063, 1075, 1150
FatI CATG 9 cut(s) 156, 237, 496, 809, 938, 976, 1059, 1071, 1146
FauI CCCGC 4 cut(s) 192, 250, 698, 896
FbaI TGATCA 1 cut(s) 499
FblI GTMKAC 1 cut(s) 114
FokI GGATG 3 cut(s) 17, 132, 611
FriOI GRGCYC 1 cut(s) 911
FspBI CTAG 2 cut(s) 365, 1179
GlaI GCGC 1 cut(s) 794
GsaI CCCAGC 2 cut(s) 789, 1236
GsuI CTGGAG 1 cut(s) 631
HaeIII GGCC 4 cut(s) 135, 176, 1046, 1183
HapII CCGG 1 cut(s) 1003
HgaI GACGC 1 cut(s) 1151
HhaI GCGC 1 cut(s) 795
Hin1I GRCGYC 1 cut(s) 1143
Hin1II CATG 9 cut(s) 160, 241, 500, 813, 942, 980, 1063, 1075, 1150
Hin6I GCGC 1 cut(s) 793
HinP1I GCGC 1 cut(s) 793
HincII GTYRAC 1 cut(s) 711
HindII GTYRAC 1 cut(s) 711
HinfI GANTC 7 cut(s) 440, 481, 554, 722, 845, 946, 1081
HpaII CCGG 1 cut(s) 1003
HphI GGTGA 5 cut(s) 157, 173, 707, 720, 1294
Hpy166II GTNNAC 6 cut(s) 115, 589, 711, 807, 1141, 1307
Hpy188I TCNGA 8 cut(s) 17, 361, 480, 559, 844, 951, 1078, 1246
Hpy188III TCNNGA 7 cut(s) 126, 140, 512, 530, 545, 648, 1003
Hpy8I GTNNAC 6 cut(s) 115, 589, 711, 807, 1141, 1307
HpyAV CCTTC 3 cut(s) 326, 365, 1027
HpyCH4III ACNGT 1 cut(s) 706
HpyF10VI GCNNNNNNNGC 7 cut(s) 270, 335, 618, 790, 932, 996, 1046
HpyF3I CTNAG 6 cut(s) 224, 339, 558, 856, 910, 1077
Hsp92I GRCGYC 1 cut(s) 1143
Hsp92II CATG 9 cut(s) 160, 241, 500, 813, 942, 980, 1063, 1075, 1150
HspAI GCGC 1 cut(s) 793
Kpn2I TCCGGA 1 cut(s) 1002
Ksp22I TGATCA 1 cut(s) 499
KspI CCGCGG 1 cut(s) 1050
Kzo9I GATC 8 cut(s) 208, 356, 394, 464, 499, 973, 1159, 1196
LguI GCTCTTC 1 cut(s) 980
LmnI GCTCC 7 cut(s) 270, 335, 481, 626, 650, 906, 1221
LweI GCATC 4 cut(s) 154, 417, 782, 1334
MaeI CTAG 2 cut(s) 365, 1179
MalI GATC 8 cut(s) 210, 358, 396, 466, 501, 975, 1161, 1198
MboI GATC 8 cut(s) 208, 356, 394, 464, 499, 973, 1159, 1196
MflI RGATCY 1 cut(s) 464
MhlI GDGCHC 2 cut(s) 911, 1226
MlsI TGGCCA 2 cut(s) 135, 176
MluCI AATT 4 cut(s) 38, 318, 1096, 1201
MluNI TGGCCA 2 cut(s) 135, 176
MlyI GAGTC 5 cut(s) 434, 475, 548, 731, 955
MmeI TCCRAC 3 cut(s) 503, 767, 773
Mox20I TGGCCA 2 cut(s) 135, 176
Mph1103I ATGCAT 1 cut(s) 1073
MroI TCCGGA 1 cut(s) 1002
MscI TGGCCA 2 cut(s) 135, 176
MseI TTAA 3 cut(s) 762, 1065, 1191
Msp20I TGGCCA 2 cut(s) 135, 176
MspA1I CMGCKG 2 cut(s) 1049, 1280
MspI CCGG 1 cut(s) 1003
MspR9I CCNGG 1 cut(s) 1185
Mva1269I GAATGC 1 cut(s) 1296
MvaI CCWGG 1 cut(s) 1185
MvnI CGCG 1 cut(s) 1049
MwoI GCNNNNNNNGC 7 cut(s) 270, 335, 618, 790, 932, 996, 1046
NdeII GATC 8 cut(s) 208, 356, 394, 464, 499, 973, 1159, 1196
NlaIII CATG 9 cut(s) 160, 241, 500, 813, 942, 980, 1063, 1075, 1150
NlaIV GGNNCC 1 cut(s) 454
NsiI ATGCAT 1 cut(s) 1073
NspI RCATGY 2 cut(s) 241, 1075
PaeI GCATGC 1 cut(s) 241
PaeR7I CTCGAG 1 cut(s) 140
PceI AGGCCT 1 cut(s) 1183
PciSI GCTCTTC 1 cut(s) 980
PcsI WCGNNNNNNNCGW 2 cut(s) 303, 822
PctI GAATGC 1 cut(s) 1296
PfeI GAWTC 2 cut(s) 845, 1081
PflFI GACNNNGTC 1 cut(s) 706
PflMI CCANNNNNTGG 1 cut(s) 1091
PleI GAGTC 5 cut(s) 434, 475, 548, 730, 954
PpsI GAGTC 5 cut(s) 434, 475, 548, 730, 954
Psp124BI GAGCTC 1 cut(s) 911
Psp6I CCWGG 1 cut(s) 1183
PspFI CCCAGC 2 cut(s) 785, 1232
PspGI CCWGG 1 cut(s) 1183
PspN4I GGNNCC 1 cut(s) 454
PspPI GGNCC 1 cut(s) 453
PstI CTGCAG 3 cut(s) 385, 867, 1030
PsuI RGATCY 1 cut(s) 464
PsyI GACNNNGTC 1 cut(s) 706
RsaI GTAC 2 cut(s) 251, 348
RsaNI GTAC 2 cut(s) 250, 347
SacI GAGCTC 1 cut(s) 911
SacII CCGCGG 1 cut(s) 1050
SapI GCTCTTC 1 cut(s) 980
SaqAI TTAA 3 cut(s) 762, 1065, 1191
Sau3AI GATC 8 cut(s) 208, 356, 394, 464, 499, 973, 1159, 1196
Sau96I GGNCC 1 cut(s) 453
SchI GAGTC 5 cut(s) 434, 475, 548, 731, 955
ScrFI CCNGG 1 cut(s) 1185
SduI GDGCHC 2 cut(s) 911, 1226
SfaNI GCATC 4 cut(s) 154, 417, 782, 1334
SfcI CTRYAG 3 cut(s) 381, 863, 1026
Sfr274I CTCGAG 1 cut(s) 140
Sfr303I CCGCGG 1 cut(s) 1050
SgrBI CCGCGG 1 cut(s) 1050
SinI GGWCC 1 cut(s) 453
SlaI CTCGAG 1 cut(s) 140
SmlI CTYRAG 3 cut(s) 124, 140, 350
SmoI CTYRAG 3 cut(s) 124, 140, 350
SphI GCATGC 1 cut(s) 241
Sse9I AATT 4 cut(s) 38, 318, 1096, 1201
SseBI AGGCCT 1 cut(s) 1183
SsiI CCGC 9 cut(s) 185, 243, 691, 731, 903, 1040, 1047, 1049, 1280
SspMI CTAG 2 cut(s) 365, 1179
SstI GAGCTC 1 cut(s) 911
StuI AGGCCT 1 cut(s) 1183
StyD4I CCNGG 1 cut(s) 1183
StyI CCWWGG 3 cut(s) 1085, 1178, 1227
TaaI ACNGT 1 cut(s) 706
TaqI TCGA 6 cut(s) 141, 484, 511, 672, 816, 1162
TasI AATT 4 cut(s) 38, 318, 1096, 1201
TatI WGTACW 2 cut(s) 249, 346
TauI GCSGC 1 cut(s) 1049
TfiI GAWTC 2 cut(s) 845, 1081
Tru1I TTAA 3 cut(s) 762, 1065, 1191
Tru9I TTAA 3 cut(s) 762, 1065, 1191
TscAI CASTG 2 cut(s) 867, 1035
TspDTI ATGAA 1 cut(s) 1163
TspRI CASTG 2 cut(s) 867, 1035
Tth111I GACNNNGTC 1 cut(s) 706
Van91I CCANNNNNTGG 1 cut(s) 1091
VpaK11BI GGWCC 1 cut(s) 453
XapI RAATTY 2 cut(s) 1096, 1201
XceI RCATGY 2 cut(s) 241, 1075
XhoI CTCGAG 1 cut(s) 140
XmaJI CCTAGG 1 cut(s) 1178
XmiI GTMKAC 1 cut(s) 114
XspI CTAG 2 cut(s) 365, 1179
Zsp2I ATGCAT 1 cut(s) 1073
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.