Rh2CG257400

Protein UPSTREAM OF

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0011027
Physical Location & Seq
Reverse (-)
27439765 .. 27441804
2040 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG257400.1

Sequence Viewer

Length: 1176 bp
ATGGATGTTCGATTTCGAAGACCCCAAACCAGTCCAGATAGAGCCAAAGTGTGCATGCAGCAACTGCAGCCAAGAGTAGCAGTCAACAAACCCACCATCAAAAAGGTCCAAATTGTCTACTACCTCTCAAGAAATGGTCATCTTGAGCATCCTCACTACATGGAGATCACCCATTTGCCCAATCAACCTCTTCGTTTGAGAGATGTAATGGATAGGCTCACTGCTCTCAGAGGCAAGGGCATGCCCTCACTATATTCCTGGTCCTGCAAAAGGAGCTACAAGAACGGTTATGTGTGGAATGATTTAGCAGAAAACGACATAATCTACCCAGCAGACGGAGCTGAGTATGTACTTAAAGGCTCTGAGCTAGTAGAAGGCTGCTCTGAGAGATTGAACCAGATTCAAATAAGCAGCCCCCAACAAATTCAAGACTCAAATTTTGGGTCCAAGAGGAGATCACCACCACTTGCTCCAACCCCAAACAGTAACCACAAGTTTCATGAAGACCATGATCAATATGATGAACAAGAAGAAGAAGAAGTTGACTATGAGGACGAGAAAACAAGCTACACAAGCTCCACAACAACAAACTCTCGCTGCTCCAGAGGAGTCTCCACAGATGAACTCGAAGAACAACAACCACCGCAAACCCACAAGACTCCCCCCGAGTCATCTCCTCTCCCAAATGCACCAGCTTCAGTGTTGAACCAACAAAACCACAGCAACAATGTGTCCATGAGGTTCGAGGACGGCGACCCCATTGTTACGAAACAGAGCTACTCTGTTTCGAAGGCAGGGCAGGGCAAGAACTCTGTTCTGCTCCAGCTCATTGCTTGCGGCAGCTCGGCCGTGGCTAAGGCCAAAAGCACACCGTGCGTGAGTCAAGGGATTGTGAAGAGGGAGAGGAGTAGTCAGAGTTTTCGTAAAGAGGTGGTGTACAGGAGTGCGGTGAAGGTTGCCATAGAGGATGAGGATATGATTACTTACATGTCTGAGAATCCAAGGTTTGGGAATTTACAGGCGGAGGAGAAGGAGTATTTCAGTGGGAGCATTGTGGAGTCCATGAAGGAAGATCAAAGAGTACATTCAGCTGAGCCTGTGCTCAAAAAATCCAATTCCTATAATGAAGAAAGGTCAGTTTTCATGTTCTTCATGCAATATGCATATAATATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF966

Protein Analysis

391

Amino Acids

44.54

Weight (kDa)

6.24

Isoelectric Point (pI)

65.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SOK PF06136 36 - 124 8.5e-48 SOSEKI protein DIX-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011027)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10150 AT5G10150 AT5G10150 AT5G10150
fragaria_vesca FvH4_1g21340 FvH4_1g21340
malus_domestica MD01G1025500.v1.1
prunus_persica Prupe.6G181200_v2.0.a1
pyrus_communis pycom01g05360 pycom01g05370
rosa_chinensis RchiOBHm_Chr2g0115201
rosa_laevigata RLG00000018165
rosa_multiflora Rmu_sc0002784.1_g000027
rosa_roxburghii Rroxscaffold_2G00130670
rosa_rugosa Rorug02G0194300
rosa_samantha Rh2AG252800 Rh2BG262400 Rh2CG257400 Rh2DG261000
rosa_wichuraiana Rw2G019720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1007
AccI GTMKAC 1 cut(s) 117
AciI CCGC 4 cut(s) 644, 837, 947, 1022
AcoI YGGCCR 1 cut(s) 846
AcsI RAATTY 3 cut(s) 423, 436, 1012
AcuI CTGAAG 1 cut(s) 681
AdeI CACNNNGTG 1 cut(s) 873
AfaI GTAC 3 cut(s) 351, 938, 1083
AfiI CCNNNNNNNGG 3 cut(s) 270, 335, 1007
AflIII ACRYGT 1 cut(s) 987
AgsI TTSAA 4 cut(s) 394, 404, 428, 706
AjnI CCWGG 1 cut(s) 257
Alw21I GWGCWC 1 cut(s) 1104
Alw26I GTCTC 1 cut(s) 616
AlwNI CAGNNNCTG 1 cut(s) 64
Ama87I CYCGRG 1 cut(s) 665
AoxI GGCC 2 cut(s) 846, 858
ApeKI GCWGC 6 cut(s) 58, 67, 378, 411, 597, 840
ApoI RAATTY 3 cut(s) 423, 436, 1012
ArsI GACNNNNNNTTYG 4 cut(s) 422, 428, 454, 460
AspS9I GGNCC 3 cut(s) 106, 261, 444
AsuHPI GGTGA 3 cut(s) 160, 450, 961
AsuII TTCGAA 2 cut(s) 16, 788
AvaI CYCGRG 1 cut(s) 665
AvaII GGWCC 3 cut(s) 106, 261, 444
BbsI GAAGAC 2 cut(s) 25, 510
Bbv12I GWGCWC 1 cut(s) 1104
BbvI GCAGC 6 cut(s) 70, 79, 365, 423, 584, 852
BccI CCATC 1 cut(s) 104
BceAI ACGGC 2 cut(s) 766, 833
BciT130I CCWGG 1 cut(s) 259
BclI TGATCA 1 cut(s) 511
BcoDI GTCTC 1 cut(s) 616
BfaI CTAG 1 cut(s) 368
BfmI CTRYAG 1 cut(s) 65
BisI GCNGC 7 cut(s) 59, 68, 379, 412, 598, 838, 841
BlpI GCTNAGC 1 cut(s) 1092
BlsI GCNGC 7 cut(s) 60, 69, 380, 413, 599, 839, 842
Bme1390I CCNGG 1 cut(s) 259
Bme18I GGWCC 3 cut(s) 106, 261, 444
BmeT110I CYCGRG 1 cut(s) 665
BmgT120I GGNCC 3 cut(s) 106, 261, 444
BmiI GGNNCC 1 cut(s) 445
BmrFI CCNGG 1 cut(s) 259
BmsI GCATC 1 cut(s) 157
BpiI GAAGAC 2 cut(s) 25, 510
BpmI CTGGAG 2 cut(s) 586, 806
Bpu10I CCTNAGC 1 cut(s) 855
Bpu1102I GCTNAGC 1 cut(s) 1092
Bpu14I TTCGAA 2 cut(s) 16, 788
BpuEI CTTGAG 2 cut(s) 112, 164
BsaJI CCNNGG 2 cut(s) 849, 1001
BsaXI ACNNNNNCTCC 4 cut(s) 560, 590, 1019, 1049
Bsc4I CCNNNNNNNGG 3 cut(s) 270, 335, 1007
Bse1I ACTGG 1 cut(s) 30
Bse3DI GCAATG 1 cut(s) 828
BseBI CCWGG 1 cut(s) 259
BseDI CCNNGG 2 cut(s) 849, 1001
BseGI GGATG 3 cut(s) 10, 148, 973
BseLI CCNNNNNNNGG 3 cut(s) 270, 335, 1007
BseMI GCAATG 1 cut(s) 828
BseMII CTCAG 6 cut(s) 241, 333, 354, 375, 984, 1083
BseNI ACTGG 1 cut(s) 30
BseRI GAGGAG 5 cut(s) 466, 621, 666, 919, 1040
BseX3I CGGCCG 1 cut(s) 846
BseXI GCAGC 6 cut(s) 70, 79, 365, 423, 584, 852
BseYI CCCAGC 1 cut(s) 328
Bsh1285I CGRYCG 1 cut(s) 849
BshFI GGCC 2 cut(s) 848, 860
BsiEI CGRYCG 1 cut(s) 849
BsiHKAI GWGCWC 1 cut(s) 1104
BsiHKCI CYCGRG 1 cut(s) 665
BslI CCNNNNNNNGG 3 cut(s) 270, 335, 1007
BsmAI GTCTC 1 cut(s) 616
BsnI GGCC 2 cut(s) 848, 860
BsoBI CYCGRG 1 cut(s) 665
Bsp119I TTCGAA 2 cut(s) 16, 788
Bsp1286I GDGCHC 1 cut(s) 1104
Bsp1407I TGTACA 1 cut(s) 936
Bsp143I GATC 4 cut(s) 165, 455, 511, 1072
Bsp1720I GCTNAGC 1 cut(s) 1092
BspACI CCGC 4 cut(s) 644, 837, 947, 1022
BspANI GGCC 2 cut(s) 848, 860
BspCNI CTCAG 6 cut(s) 240, 334, 355, 376, 985, 1084
BspHI TCATGA 1 cut(s) 499
BspLI GGNNCC 1 cut(s) 445
BspMAI CTGCAG 1 cut(s) 69
BspT104I TTCGAA 2 cut(s) 16, 788
BsrDI GCAATG 1 cut(s) 828
BsrGI TGTACA 1 cut(s) 936
BsrI ACTGG 1 cut(s) 30
BssECI CCNNGG 2 cut(s) 849, 1001
BssMI GATC 4 cut(s) 165, 455, 511, 1072
BssT1I CCWWGG 1 cut(s) 1001
Bst2UI CCWGG 1 cut(s) 259
Bst4CI ACNGT 3 cut(s) 287, 485, 873
Bst6I CTCTTC 2 cut(s) 195, 890
BstAPI GCANNNNNTGC 2 cut(s) 64, 873
BstAUI TGTACA 1 cut(s) 936
BstBI TTCGAA 2 cut(s) 16, 788
BstC8I GCNNGC 3 cut(s) 56, 242, 835
BstDEI CTNAG 7 cut(s) 227, 342, 363, 384, 855, 993, 1092
BstDSI CCRYGG 1 cut(s) 849
BstENI CCTNNNNNAGG 1 cut(s) 268
BstF5I GGATG 3 cut(s) 10, 148, 973
BstKTI GATC 4 cut(s) 168, 458, 514, 1075
BstMAI GTCTC 1 cut(s) 616
BstMBI GATC 4 cut(s) 165, 455, 511, 1072
BstMCI CGRYCG 1 cut(s) 849
BstMWI GCNNNNNNNGC 6 cut(s) 64, 67, 273, 338, 573, 873
BstNI CCWGG 1 cut(s) 259
BstNSI RCATGY 3 cut(s) 58, 244, 991
BstSCI CCNGG 1 cut(s) 257
BstSFI CTRYAG 1 cut(s) 65
BstV1I GCAGC 6 cut(s) 70, 79, 365, 423, 584, 852
BstV2I GAAGAC 2 cut(s) 25, 510
BstZI CGGCCG 1 cut(s) 846
BsuRI GGCC 2 cut(s) 848, 860
BtgI CCRYGG 1 cut(s) 849
BtsCI GGATG 3 cut(s) 10, 148, 973
BtsI GCAGTG 1 cut(s) 219
BtsIMutI CAGTG 3 cut(s) 219, 705, 1048
Cac8I GCNNGC 3 cut(s) 56, 242, 835
CaiI CAGNNNCTG 1 cut(s) 64
CciI TCATGA 1 cut(s) 499
Cfr13I GGNCC 3 cut(s) 106, 261, 444
Csp6I GTAC 3 cut(s) 350, 937, 1082
CviQI GTAC 3 cut(s) 350, 937, 1082
DdeI CTNAG 7 cut(s) 227, 342, 363, 384, 855, 993, 1092
DpnI GATC 4 cut(s) 167, 457, 513, 1074
DpnII GATC 4 cut(s) 165, 455, 511, 1072
DraIII CACNNNGTG 1 cut(s) 873
EaeI YGGCCR 1 cut(s) 846
EagI CGGCCG 1 cut(s) 846
Eam1104I CTCTTC 2 cut(s) 195, 890
EarI CTCTTC 2 cut(s) 195, 890
EciI GGCGGA 1 cut(s) 1037
EclXI CGGCCG 1 cut(s) 846
Eco130I CCWWGG 1 cut(s) 1001
Eco47I GGWCC 3 cut(s) 106, 261, 444
Eco52I CGGCCG 1 cut(s) 846
Eco57I CTGAAG 1 cut(s) 681
Eco88I CYCGRG 1 cut(s) 665
EcoNI CCTNNNNNAGG 1 cut(s) 268
EcoRII CCWGG 1 cut(s) 257
EcoT14I CCWWGG 1 cut(s) 1001
EcoT22I ATGCAT 1 cut(s) 1165
ErhI CCWWGG 1 cut(s) 1001
FbaI TGATCA 1 cut(s) 511
FblI GTMKAC 1 cut(s) 117
Fnu4HI GCNGC 7 cut(s) 59, 68, 379, 412, 598, 838, 841
FokI GGATG 3 cut(s) 17, 135, 980
Fsp4HI GCNGC 7 cut(s) 59, 68, 379, 412, 598, 838, 841
FspBI CTAG 1 cut(s) 368
GluI GCNGC 7 cut(s) 59, 68, 379, 412, 598, 838, 841
GsaI CCCAGC 1 cut(s) 332
GsuI CTGGAG 2 cut(s) 586, 806
HaeIII GGCC 2 cut(s) 848, 860
HincII GTYRAC 2 cut(s) 85, 544
HindII GTYRAC 2 cut(s) 85, 544
HinfI GANTC 8 cut(s) 400, 431, 609, 658, 668, 880, 997, 1058
HphI GGTGA 3 cut(s) 160, 450, 961
Hpy166II GTNNAC 4 cut(s) 85, 118, 544, 937
Hpy188I TCNGA 5 cut(s) 230, 364, 385, 915, 994
Hpy188III TCNNGA 6 cut(s) 35, 129, 143, 428, 500, 603
Hpy8I GTNNAC 4 cut(s) 85, 118, 544, 937
HpyAV CCTTC 5 cut(s) 368, 784, 946, 1024, 1060
HpyCH4III ACNGT 3 cut(s) 287, 485, 873
HpyCH4V TGCA 7 cut(s) 54, 58, 67, 267, 689, 1156, 1163
HpyF10VI GCNNNNNNNGC 6 cut(s) 64, 67, 273, 338, 573, 873
HpyF3I CTNAG 7 cut(s) 227, 342, 363, 384, 855, 993, 1092
Ksp22I TGATCA 1 cut(s) 511
Kzo9I GATC 4 cut(s) 165, 455, 511, 1072
LmnI GCTCC 7 cut(s) 273, 338, 475, 581, 605, 825, 1047
Lsp1109I GCAGC 6 cut(s) 70, 79, 365, 423, 584, 852
LweI GCATC 1 cut(s) 157
MaeI CTAG 1 cut(s) 368
MaeIII GTNAC 2 cut(s) 485, 763
MalI GATC 4 cut(s) 167, 457, 513, 1074
MboI GATC 4 cut(s) 165, 455, 511, 1072
MhlI GDGCHC 1 cut(s) 1104
MluCI AATT 5 cut(s) 111, 423, 436, 1012, 1114
MlyI GAGTC 6 cut(s) 425, 618, 652, 677, 889, 1067
MmeI TCCRAC 1 cut(s) 497
Mph1103I ATGCAT 1 cut(s) 1165
MseI TTAA 1 cut(s) 354
MspA1I CMGCKG 1 cut(s) 1091
MspR9I CCNGG 1 cut(s) 259
MvaI CCWGG 1 cut(s) 259
MwoI GCNNNNNNNGC 6 cut(s) 64, 67, 273, 338, 573, 873
NdeII GATC 4 cut(s) 165, 455, 511, 1072
NlaIV GGNNCC 1 cut(s) 445
NmeAIII GCCGAG 1 cut(s) 824
NsiI ATGCAT 1 cut(s) 1165
NspI RCATGY 3 cut(s) 58, 244, 991
NspV TTCGAA 2 cut(s) 16, 788
PaeI GCATGC 2 cut(s) 58, 244
PagI TCATGA 1 cut(s) 499
PciI ACATGT 1 cut(s) 987
PcsI WCGNNNNNNNCGW 1 cut(s) 750
PfeI GAWTC 2 cut(s) 400, 997
PflMI CCANNNNNTGG 1 cut(s) 1007
PkrI GCNGC 7 cut(s) 60, 69, 380, 413, 599, 839, 842
PleI GAGTC 6 cut(s) 425, 617, 652, 676, 888, 1066
PpsI GAGTC 6 cut(s) 425, 617, 652, 676, 888, 1066
PscI ACATGT 1 cut(s) 987
Psp6I CCWGG 1 cut(s) 257
PspFI CCCAGC 1 cut(s) 328
PspGI CCWGG 1 cut(s) 257
PspN4I GGNNCC 1 cut(s) 445
PspPI GGNCC 3 cut(s) 106, 261, 444
PstI CTGCAG 1 cut(s) 69
PstNI CAGNNNCTG 1 cut(s) 64
PvuII CAGCTG 1 cut(s) 1091
RsaI GTAC 3 cut(s) 351, 938, 1083
RsaNI GTAC 3 cut(s) 350, 937, 1082
SaqAI TTAA 1 cut(s) 354
SatI GCNGC 7 cut(s) 59, 68, 379, 412, 598, 838, 841
Sau3AI GATC 4 cut(s) 165, 455, 511, 1072
Sau96I GGNCC 3 cut(s) 106, 261, 444
SchI GAGTC 6 cut(s) 425, 618, 652, 677, 889, 1067
ScrFI CCNGG 1 cut(s) 259
SduI GDGCHC 1 cut(s) 1104
SfaNI GCATC 1 cut(s) 157
SfcI CTRYAG 1 cut(s) 65
SfuI TTCGAA 2 cut(s) 16, 788
SinI GGWCC 3 cut(s) 106, 261, 444
SmlI CTYRAG 2 cut(s) 127, 143
SmoI CTYRAG 2 cut(s) 127, 143
SphI GCATGC 2 cut(s) 58, 244
Sse9I AATT 5 cut(s) 111, 423, 436, 1012, 1114
SsiI CCGC 4 cut(s) 644, 837, 947, 1022
SspI AATATT 1 cut(s) 1171
SspMI CTAG 1 cut(s) 368
StyD4I CCNGG 1 cut(s) 257
StyI CCWWGG 1 cut(s) 1001
TaaI ACNGT 3 cut(s) 287, 485, 873
TaqI TCGA 5 cut(s) 10, 16, 627, 744, 788
TasI AATT 5 cut(s) 111, 423, 436, 1012, 1114
TatI WGTACW 3 cut(s) 349, 936, 1081
TauI GCSGC 1 cut(s) 840
TfiI GAWTC 2 cut(s) 400, 997
Tru1I TTAA 1 cut(s) 354
Tru9I TTAA 1 cut(s) 354
TscAI CASTG 3 cut(s) 226, 705, 1048
TseI GCWGC 6 cut(s) 58, 67, 378, 411, 597, 840
TspDTI ATGAA 8 cut(s) 488, 516, 537, 636, 1079, 1132, 1140, 1141
TspGWI ACGGA 1 cut(s) 351
TspRI CASTG 3 cut(s) 226, 705, 1048
Van91I CCANNNNNTGG 1 cut(s) 1007
VpaK11BI GGWCC 3 cut(s) 106, 261, 444
XagI CCTNNNNNAGG 1 cut(s) 268
XapI RAATTY 3 cut(s) 423, 436, 1012
XceI RCATGY 3 cut(s) 58, 244, 991
XmiI GTMKAC 1 cut(s) 117
XspI CTAG 1 cut(s) 368
Zsp2I ATGCAT 1 cut(s) 1165
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.