AT5G10620

RNA methyltransferase At5g10620

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0015123
Physical Location & Seq
Reverse (-)
3355006 .. 3356686
1681 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G10620.1

Sequence Viewer

Length: 582 bp
ATGTCCATCTCTGTCATGGCCAATTCTCACCTGAATCAGCCATATGCATCTGCTTCTCCTACTCCTCAGGAAAAAGGAAGAACTTGTAGATACGCGGGTCAAGCAGTGAGAGCTCTTCCTATACGAGTAATAACAGTGGGGAAAAAGAGATCAGAAGGTGTTCGACTCTTGGTCGATGAATACAAAATCAAGCTTAAACCTTACTGTTCCTTTGAAGATTCATTGGTTCGCTCCAATCCTCGAAATGCTCAGGATGTTAGAGCTCAGGTCGAGGATGAAGAAGTGGCTATGATGAAGCTTATTGGGTCTGATGATTGGGTTGTGGTGCTTGACGAGAGAGGCCGCGACATTGATTCAGAGCAGATGGCTGAGTTACTTGGGGATGCAGGAAACAGTGGAGCTTCGCGGATCTCGTTTTGTATAGGTGGAGCTTACGGACATGGAACGCAAGTGAGGAAGCGAGCTAATGTGACCATTAGACTTTCCTCCATGGTCCTGAATCATCAGATAGCTCTTGTGGTGCTCATGGAACAGCTTTACAGGTCATGGACTATTCTCAAGGGACAAAATTACCATCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SPOUT_MTase

Protein Analysis

193

Amino Acids

21.53

Weight (kDa)

9.01

Isoelectric Point (pI)

34.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPOUT_MTase PF02590 41 - 192 2e-46 Predicted SPOUT methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015123)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10620 AT5G10620
fragaria_vesca FvH4_2g30670
malus_domestica MD15G1110100.v1.1
prunus_persica Prupe.1G462300_v2.0.a1
pyrus_communis pycom15g10030
rosa_chinensis RchiOBHm_Chr6g0312221
rosa_laevigata RLG00000010341
rosa_multiflora Rmu_sc0010924.1_g000004
rosa_roxburghii Rroxscaffold_7G00156940
rosa_rugosa Rorug06G0394200
rosa_samantha Rh6AG511100 Rh6BG522100 Rh6CG527900 Rh6DG513300
rosa_wichuraiana Rw6G044450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 95, 345, 406
AciI CCGC 3 cut(s) 95, 343, 406
AclWI GGATC 1 cut(s) 416
AcoI YGGCCR 1 cut(s) 18
AgsI TTSAA 1 cut(s) 215
AhdI GACNNNNNGTC 1 cut(s) 170
AluBI AGCT 9 cut(s) 113, 193, 263, 298, 401, 431, 464, 512, 535
AluI AGCT 9 cut(s) 113, 193, 263, 298, 401, 431, 464, 512, 535
Alw21I GWGCWC 3 cut(s) 115, 265, 525
AlwI GGATC 1 cut(s) 416
AoxI GGCC 2 cut(s) 18, 340
Asp700I GAANNNNTTC 1 cut(s) 159
AspS9I GGNCC 1 cut(s) 493
AsuHPI GGTGA 1 cut(s) 20
AvaII GGWCC 1 cut(s) 493
AxyI CCTNAGG 1 cut(s) 66
BaeI ACNNNNGTAYC 2 cut(s) 82, 115
BalI TGGCCA 1 cut(s) 20
BanII GRGCYC 2 cut(s) 115, 265
Bbv12I GWGCWC 3 cut(s) 115, 265, 525
BccI CCATC 2 cut(s) 14, 358
BisI GCNGC 1 cut(s) 343
BlsI GCNGC 1 cut(s) 344
Bme18I GGWCC 1 cut(s) 493
BmeRI GACNNNNNGTC 1 cut(s) 170
BmgT120I GGNCC 1 cut(s) 493
BmsI GCATC 2 cut(s) 56, 373
Bpu10I CCTNAGC 2 cut(s) 249, 264
BpuEI CTTGAG 1 cut(s) 542
BsaJI CCNNGG 1 cut(s) 489
Bse21I CCTNAGG 1 cut(s) 66
BseDI CCNNGG 1 cut(s) 489
BseGI GGATG 3 cut(s) 259, 280, 388
BseMII CTCAG 4 cut(s) 80, 263, 278, 360
BseRI GAGGAG 1 cut(s) 54
Bsh1236I CGCG 3 cut(s) 95, 345, 406
BshFI GGCC 2 cut(s) 20, 342
BsiHKAI GWGCWC 3 cut(s) 115, 265, 525
BslFI GGGAC 1 cut(s) 576
BsmFI GGGAC 1 cut(s) 576
BsnI GGCC 2 cut(s) 20, 342
Bsp1286I GDGCHC 3 cut(s) 115, 265, 525
Bsp143I GATC 2 cut(s) 149, 408
Bsp19I CCATGG 1 cut(s) 489
BspACI CCGC 3 cut(s) 95, 343, 406
BspANI GGCC 2 cut(s) 20, 342
BspCNI CTCAG 4 cut(s) 79, 262, 277, 361
BspFNI CGCG 3 cut(s) 95, 345, 406
BspPI GGATC 1 cut(s) 416
BspQI GCTCTTC 1 cut(s) 120
BssECI CCNNGG 1 cut(s) 489
BssMI GATC 2 cut(s) 149, 408
BssT1I CCWWGG 1 cut(s) 489
Bst4CI ACNGT 3 cut(s) 136, 206, 395
Bst6I CTCTTC 1 cut(s) 120
BstC8I GCNNGC 1 cut(s) 462
BstDEI CTNAG 4 cut(s) 66, 249, 264, 369
BstDSI CCRYGG 1 cut(s) 489
BstF5I GGATG 3 cut(s) 259, 280, 388
BstFNI CGCG 3 cut(s) 95, 345, 406
BstKTI GATC 2 cut(s) 152, 411
BstMBI GATC 2 cut(s) 149, 408
BstMWI GCNNNNNNNGC 2 cut(s) 101, 110
BstUI CGCG 3 cut(s) 95, 345, 406
BstX2I RGATCY 1 cut(s) 408
BstYI RGATCY 1 cut(s) 408
Bsu36I CCTNAGG 1 cut(s) 66
BsuRI GGCC 2 cut(s) 20, 342
BtgI CCRYGG 1 cut(s) 489
BtsCI GGATG 3 cut(s) 259, 280, 388
BtsI GCAGTG 1 cut(s) 111
BtsIMutI CAGTG 4 cut(s) 111, 141, 400, 577
Cac8I GCNNGC 1 cut(s) 462
Cfr13I GGNCC 1 cut(s) 493
CviAII CATG 5 cut(s) 16, 440, 490, 526, 546
DdeI CTNAG 4 cut(s) 66, 249, 264, 369
DpnI GATC 2 cut(s) 151, 410
DpnII GATC 2 cut(s) 149, 408
DriI GACNNNNNGTC 1 cut(s) 170
EaeI YGGCCR 1 cut(s) 18
Eam1104I CTCTTC 1 cut(s) 120
Eam1105I GACNNNNNGTC 1 cut(s) 170
EarI CTCTTC 1 cut(s) 120
Ecl136II GAGCTC 2 cut(s) 113, 263
Eco130I CCWWGG 1 cut(s) 489
Eco24I GRGCYC 2 cut(s) 115, 265
Eco47I GGWCC 1 cut(s) 493
Eco53kI GAGCTC 2 cut(s) 113, 263
Eco81I CCTNAGG 1 cut(s) 66
EcoICRI GAGCTC 2 cut(s) 113, 263
EcoT14I CCWWGG 1 cut(s) 489
EcoT22I ATGCAT 1 cut(s) 49
EcoT38I GRGCYC 2 cut(s) 115, 265
ErhI CCWWGG 1 cut(s) 489
FaeI CATG 5 cut(s) 19, 443, 493, 529, 549
FaqI GGGAC 1 cut(s) 576
FatI CATG 5 cut(s) 15, 439, 489, 525, 545
FauI CCCGC 1 cut(s) 88
FauNDI CATATG 1 cut(s) 43
Fnu4HI GCNGC 1 cut(s) 343
FokI GGATG 3 cut(s) 266, 287, 395
FriOI GRGCYC 2 cut(s) 115, 265
Fsp4HI GCNGC 1 cut(s) 343
GluI GCNGC 1 cut(s) 343
HaeIII GGCC 2 cut(s) 20, 342
Hin1II CATG 5 cut(s) 19, 443, 493, 529, 549
HindIII AAGCTT 2 cut(s) 191, 296
HinfI GANTC 5 cut(s) 34, 165, 218, 353, 499
HphI GGTGA 1 cut(s) 20
Hpy188I TCNGA 4 cut(s) 154, 310, 358, 507
Hpy188III TCNNGA 3 cut(s) 68, 251, 496
HpyAV CCTTC 1 cut(s) 149
HpyCH4III ACNGT 3 cut(s) 136, 206, 395
HpyCH4V TGCA 2 cut(s) 47, 386
HpyF10VI GCNNNNNNNGC 2 cut(s) 101, 110
HpyF3I CTNAG 4 cut(s) 66, 249, 264, 369
Hsp92II CATG 5 cut(s) 19, 443, 493, 529, 549
Kzo9I GATC 2 cut(s) 149, 408
LguI GCTCTTC 1 cut(s) 120
LmnI GCTCC 3 cut(s) 236, 398, 428
LpnPI CCDG 7 cut(s) 44, 53, 236, 251, 372, 509, 526
LweI GCATC 2 cut(s) 56, 373
MaeIII GTNAC 2 cut(s) 372, 469
MalI GATC 2 cut(s) 151, 410
MboI GATC 2 cut(s) 149, 408
MboII GAAGA 4 cut(s) 90, 107, 227, 290
MflI RGATCY 1 cut(s) 408
MhlI GDGCHC 3 cut(s) 115, 265, 525
MlsI TGGCCA 1 cut(s) 20
MluCI AATT 2 cut(s) 22, 568
MluNI TGGCCA 1 cut(s) 20
MlyI GAGTC 1 cut(s) 159
MnlI CCTC 6 cut(s) 75, 249, 265, 332, 447, 496
Mox20I TGGCCA 1 cut(s) 20
Mph1103I ATGCAT 1 cut(s) 49
MroXI GAANNNNTTC 1 cut(s) 159
MscI TGGCCA 1 cut(s) 20
MseI TTAA 1 cut(s) 195
Msp20I TGGCCA 1 cut(s) 20
MvnI CGCG 3 cut(s) 95, 345, 406
MwoI GCNNNNNNNGC 2 cut(s) 101, 110
NcoI CCATGG 1 cut(s) 489
NdeI CATATG 1 cut(s) 43
NdeII GATC 2 cut(s) 149, 408
NlaIII CATG 5 cut(s) 19, 443, 493, 529, 549
NmuCI GTSAC 1 cut(s) 469
NsiI ATGCAT 1 cut(s) 49
PciSI GCTCTTC 1 cut(s) 120
PcsI WCGNNNNNNNCGW 1 cut(s) 410
PdmI GAANNNNTTC 1 cut(s) 159
PfeI GAWTC 4 cut(s) 34, 218, 353, 499
PkrI GCNGC 1 cut(s) 344
PleI GAGTC 1 cut(s) 159
PpsI GAGTC 1 cut(s) 159
Psp124BI GAGCTC 2 cut(s) 115, 265
PspPI GGNCC 1 cut(s) 493
PsuI RGATCY 1 cut(s) 408
SacI GAGCTC 2 cut(s) 115, 265
SapI GCTCTTC 1 cut(s) 120
SaqAI TTAA 1 cut(s) 195
SatI GCNGC 1 cut(s) 343
Sau3AI GATC 2 cut(s) 149, 408
Sau96I GGNCC 1 cut(s) 493
SchI GAGTC 1 cut(s) 159
SduI GDGCHC 3 cut(s) 115, 265, 525
SfaNI GCATC 2 cut(s) 56, 373
SinI GGWCC 1 cut(s) 493
SmlI CTYRAG 1 cut(s) 557
SmoI CTYRAG 1 cut(s) 557
Sse9I AATT 2 cut(s) 22, 568
SsiI CCGC 3 cut(s) 95, 343, 406
SstI GAGCTC 2 cut(s) 115, 265
StyI CCWWGG 1 cut(s) 489
TaaI ACNGT 3 cut(s) 136, 206, 395
TaqI TCGA 4 cut(s) 163, 174, 241, 270
TasI AATT 2 cut(s) 22, 568
TauI GCSGC 1 cut(s) 345
TfiI GAWTC 4 cut(s) 34, 218, 353, 499
Tru1I TTAA 1 cut(s) 195
Tru9I TTAA 1 cut(s) 195
TscAI CASTG 3 cut(s) 111, 141, 400
TseFI GTSAC 1 cut(s) 469
Tsp45I GTSAC 1 cut(s) 469
TspDTI ATGAA 4 cut(s) 192, 210, 291, 308
TspGWI ACGGA 1 cut(s) 450
TspRI CASTG 3 cut(s) 111, 141, 400
VpaK11BI GGWCC 1 cut(s) 493
XcmI CCANNNNNNNNNTGG 1 cut(s) 13
XmnI GAANNNNTTC 1 cut(s) 159
Zsp2I ATGCAT 1 cut(s) 49
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.