Rh6AG511100

RNA methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0015123
Physical Location & Seq
Forward (+)
68185822 .. 68188023
2202 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG511100.1

Sequence Viewer

Length: 579 bp
ATGGTTGTGGTGCTTAGTGCCTCTGCTACCAATTTCTCTGCACTTCATTCAGGTTCCTGTTTTCTCGAAAGCCTTGCATCAACTCTATCCTTCCTCATATACTTTATGAGTTTGGCATTTACAAACTTGATTTTGTTTCGGATTTCAGGTAAAGGTTGCAAGTACACAGGTCAAGCAGTGAGAGCACTTCCTATTCGCATAATATCGGTGGGGAAAAAGAGATCACAAGGTGTACAACTCGTTGTGGATGACTACATTCAAAGGCTTAAGTTATACTGCAGTGTTGAGGATGTTCACATAAAGAACAATCCTAAGAATGCAAGTGATTGGAGGGCTCAGGTAGATCATGAAGACGGTGCTGTCATGGATCTTATCAGGTCCGATGATTGGGTGGTGCTGTTGGATGAGCGTGGGAAAGACATAGGTTCCATGCAGATGGCTGAGCTGATCGGAGATGCAGGGAATACGACTAATAGATGTATTCAGATGTTCGACTGCGGCAACATAGAAGATCAAGTTCGTCATAATATCATTGTTATGCGCATCAATGATCATTTTCCGTGTCTCCTTTCAGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SPOUT_MTase

Protein Analysis

192

Amino Acids

21.39

Weight (kDa)

6.18

Isoelectric Point (pI)

27.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPOUT_MTase PF02590 64 - 161 1.8e-16 Predicted SPOUT methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015123)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10620 AT5G10620
fragaria_vesca FvH4_2g30670
malus_domestica MD15G1110100.v1.1
prunus_persica Prupe.1G462300_v2.0.a1
pyrus_communis pycom15g10030
rosa_chinensis RchiOBHm_Chr6g0312221
rosa_laevigata RLG00000010341
rosa_multiflora Rmu_sc0010924.1_g000004
rosa_roxburghii Rroxscaffold_7G00156940
rosa_rugosa Rorug06G0394200
rosa_samantha Rh6AG511100 Rh6BG522100 Rh6CG527900 Rh6DG513300
rosa_wichuraiana Rw6G044450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 359
Acc16I TGCGCA 1 cut(s) 542
AciI CCGC 1 cut(s) 498
AclWI GGATC 1 cut(s) 375
AdeI CACNNNGTG 1 cut(s) 230
AfaI GTAC 2 cut(s) 164, 234
AfiI CCNNNNNNNGG 1 cut(s) 387
AflII CTTAAG 1 cut(s) 266
AgsI TTSAA 1 cut(s) 260
AluBI AGCT 1 cut(s) 445
AluI AGCT 1 cut(s) 445
Alw21I GWGCWC 1 cut(s) 187
Alw26I GTCTC 1 cut(s) 569
AlwI GGATC 1 cut(s) 375
AspLEI GCGC 1 cut(s) 543
AspS9I GGNCC 1 cut(s) 378
AvaII GGWCC 1 cut(s) 378
BanII GRGCYC 1 cut(s) 337
BbsI GAAGAC 1 cut(s) 357
Bbv12I GWGCWC 1 cut(s) 187
BccI CCATC 1 cut(s) 430
BcgI CGANNNNNNTGC 2 cut(s) 56, 90
BclI TGATCA 1 cut(s) 550
BcoDI GTCTC 1 cut(s) 569
BfaI CTAG 1 cut(s) 577
BfmI CTRYAG 1 cut(s) 277
BfrI CTTAAG 1 cut(s) 266
BisI GCNGC 1 cut(s) 499
BlpI GCTNAGC 1 cut(s) 441
BlsI GCNGC 1 cut(s) 500
Bme18I GGWCC 1 cut(s) 378
BmgT120I GGNCC 1 cut(s) 378
BmiI GGNNCC 2 cut(s) 55, 427
BmsI GCATC 3 cut(s) 86, 445, 552
BpiI GAAGAC 1 cut(s) 357
Bpu10I CCTNAGC 1 cut(s) 336
Bpu1102I GCTNAGC 1 cut(s) 441
Bsc4I CCNNNNNNNGG 1 cut(s) 387
BseGI GGATG 3 cut(s) 253, 295, 409
BseLI CCNNNNNNNGG 1 cut(s) 387
BseMII CTCAG 2 cut(s) 350, 432
BsgI GTGCAG 1 cut(s) 24
BsiHKAI GWGCWC 1 cut(s) 187
BslI CCNNNNNNNGG 1 cut(s) 387
BsmAI GTCTC 1 cut(s) 569
BsmI GAATGC 1 cut(s) 322
Bsp1286I GDGCHC 2 cut(s) 187, 337
Bsp1407I TGTACA 1 cut(s) 232
Bsp143I GATC 6 cut(s) 221, 343, 367, 447, 511, 550
Bsp1720I GCTNAGC 1 cut(s) 441
BspACI CCGC 1 cut(s) 498
BspCNI CTCAG 2 cut(s) 349, 433
BspHI TCATGA 1 cut(s) 346
BspLI GGNNCC 2 cut(s) 55, 427
BspMAI CTGCAG 1 cut(s) 281
BspPI GGATC 1 cut(s) 375
BspTI CTTAAG 1 cut(s) 266
BsrGI TGTACA 1 cut(s) 232
BssMI GATC 6 cut(s) 221, 343, 367, 447, 511, 550
Bst4CI ACNGT 1 cut(s) 356
BstAFI CTTAAG 1 cut(s) 266
BstAUI TGTACA 1 cut(s) 232
BstDEI CTNAG 4 cut(s) 14, 312, 336, 441
BstF5I GGATG 3 cut(s) 253, 295, 409
BstHHI GCGC 1 cut(s) 543
BstKTI GATC 6 cut(s) 224, 346, 370, 450, 514, 553
BstMAI GTCTC 1 cut(s) 569
BstMBI GATC 6 cut(s) 221, 343, 367, 447, 511, 550
BstMWI GCNNNNNNNGC 1 cut(s) 182
BstSFI CTRYAG 1 cut(s) 277
BstV2I GAAGAC 1 cut(s) 357
BstX2I RGATCY 1 cut(s) 367
BstXI CCANNNNNNTGG 1 cut(s) 436
BstYI RGATCY 1 cut(s) 367
BtsCI GGATG 3 cut(s) 253, 295, 409
BtsI GCAGTG 2 cut(s) 183, 286
BtsIMutI CAGTG 2 cut(s) 183, 286
CciI TCATGA 1 cut(s) 346
CfoI GCGC 1 cut(s) 543
Cfr13I GGNCC 1 cut(s) 378
Csp6I GTAC 2 cut(s) 163, 233
CviAII CATG 3 cut(s) 347, 364, 430
CviJI RGCY 6 cut(s) 72, 265, 335, 440, 445, 575
CviKI_1 RGCY 6 cut(s) 72, 265, 335, 440, 445, 575
CviQI GTAC 2 cut(s) 163, 233
DdeI CTNAG 4 cut(s) 14, 312, 336, 441
DpnI GATC 6 cut(s) 223, 345, 369, 449, 513, 552
DpnII GATC 6 cut(s) 221, 343, 367, 447, 511, 550
DraIII CACNNNGTG 1 cut(s) 230
DrdI GACNNNNNNGTC 1 cut(s) 359
DseDI GACNNNNNNGTC 1 cut(s) 359
Eco24I GRGCYC 1 cut(s) 337
Eco47I GGWCC 1 cut(s) 378
EcoT38I GRGCYC 1 cut(s) 337
FaeI CATG 3 cut(s) 350, 367, 433
FatI CATG 3 cut(s) 346, 363, 429
FbaI TGATCA 1 cut(s) 550
Fnu4HI GCNGC 1 cut(s) 499
FokI GGATG 3 cut(s) 260, 302, 416
FriOI GRGCYC 1 cut(s) 337
Fsp4HI GCNGC 1 cut(s) 499
FspAI RTGCGCAY 1 cut(s) 542
FspBI CTAG 1 cut(s) 577
FspI TGCGCA 1 cut(s) 542
GlaI GCGC 1 cut(s) 542
GluI GCNGC 1 cut(s) 499
HhaI GCGC 1 cut(s) 543
Hin1II CATG 3 cut(s) 350, 367, 433
Hin6I GCGC 1 cut(s) 541
HinP1I GCGC 1 cut(s) 541
Hpy166II GTNNAC 3 cut(s) 165, 233, 295
Hpy188I TCNGA 4 cut(s) 141, 382, 452, 486
Hpy188III TCNNGA 2 cut(s) 65, 347
Hpy8I GTNNAC 3 cut(s) 165, 233, 295
HpyAV CCTTC 1 cut(s) 100
HpyCH4III ACNGT 1 cut(s) 356
HpyCH4V TGCA 7 cut(s) 41, 77, 159, 279, 320, 433, 458
HpyF10VI GCNNNNNNNGC 1 cut(s) 182
HpyF3I CTNAG 4 cut(s) 14, 312, 336, 441
Hsp92II CATG 3 cut(s) 350, 367, 433
HspAI GCGC 1 cut(s) 541
Ksp22I TGATCA 1 cut(s) 550
Kzo9I GATC 6 cut(s) 221, 343, 367, 447, 511, 550
LpnPI CCDG 7 cut(s) 36, 70, 132, 153, 323, 361, 444
LweI GCATC 3 cut(s) 86, 445, 552
MaeI CTAG 1 cut(s) 577
MalI GATC 6 cut(s) 223, 345, 369, 449, 513, 552
MboI GATC 6 cut(s) 221, 343, 367, 447, 511, 550
MboII GAAGA 2 cut(s) 362, 521
MflI RGATCY 1 cut(s) 367
MhlI GDGCHC 2 cut(s) 187, 337
MluCI AATT 1 cut(s) 31
MmeI TCCRAC 1 cut(s) 381
MnlI CCTC 4 cut(s) 31, 104, 280, 324
MseI TTAA 1 cut(s) 267
MslI CAYNNNNRTG 2 cut(s) 434, 536
MspCI CTTAAG 1 cut(s) 266
Mva1269I GAATGC 1 cut(s) 322
MwoI GCNNNNNNNGC 1 cut(s) 182
NdeII GATC 6 cut(s) 221, 343, 367, 447, 511, 550
NlaIII CATG 3 cut(s) 350, 367, 433
NlaIV GGNNCC 2 cut(s) 55, 427
NsbI TGCGCA 1 cut(s) 542
PagI TCATGA 1 cut(s) 346
PctI GAATGC 1 cut(s) 322
PkrI GCNGC 1 cut(s) 500
PspN4I GGNNCC 2 cut(s) 55, 427
PspPI GGNCC 1 cut(s) 378
PstI CTGCAG 1 cut(s) 281
PsuI RGATCY 1 cut(s) 367
RsaI GTAC 2 cut(s) 164, 234
RsaNI GTAC 2 cut(s) 163, 233
RseI CAYNNNNRTG 2 cut(s) 434, 536
SaqAI TTAA 1 cut(s) 267
SatI GCNGC 1 cut(s) 499
Sau3AI GATC 6 cut(s) 221, 343, 367, 447, 511, 550
Sau96I GGNCC 1 cut(s) 378
SduI GDGCHC 2 cut(s) 187, 337
SetI ASST 9 cut(s) 55, 151, 157, 172, 232, 342, 380, 427, 447
SfaNI GCATC 3 cut(s) 86, 445, 552
SfcI CTRYAG 1 cut(s) 277
SinI GGWCC 1 cut(s) 378
SmiMI CAYNNNNRTG 2 cut(s) 434, 536
SmlI CTYRAG 1 cut(s) 266
SmoI CTYRAG 1 cut(s) 266
Sse9I AATT 1 cut(s) 31
SsiI CCGC 1 cut(s) 498
SspMI CTAG 1 cut(s) 577
TaaI ACNGT 1 cut(s) 356
TaqI TCGA 2 cut(s) 66, 492
TasI AATT 1 cut(s) 31
TatI WGTACW 2 cut(s) 162, 232
TauI GCSGC 1 cut(s) 501
Tru1I TTAA 1 cut(s) 267
Tru9I TTAA 1 cut(s) 267
TscAI CASTG 2 cut(s) 183, 286
TspDTI ATGAA 2 cut(s) 35, 363
TspGWI ACGGA 1 cut(s) 549
TspRI CASTG 2 cut(s) 183, 286
Vha464I CTTAAG 1 cut(s) 266
VpaK11BI GGWCC 1 cut(s) 378
XspI CTAG 1 cut(s) 577
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.