Rh6BG522100

RNA methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0015123
Physical Location & Seq
Forward (+)
71518571 .. 71520185
1615 bp
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UTR
Exon/CDS
Intron
Rh6BG522100.1

Sequence Viewer

Length: 654 bp
ATGGTTGTGGTGCTTAGTGCCTCTGCTACCAATTTCTCTGCACTTCCTTCAGGTTACTGTTTTCTCGCAAGCCTTGCAGCAACTCTATCCTTCCTTATATACTTTATGAGTTTGGCATTTACAAACTTGATTTTGTTTCGGATTTCAGGTAAAGGTTGCAAGTACACAGGTCAAGCAGTGAGAGCACTTCCTATACGCATAATATCGGTAGGGAAAAAGAGATCACAAGGTGTACAACTCGTTGTGGATGACTACATTCAAAGGCTTAAGTTATACTCTAGTGTTGAGGATGTTCACATAAAGAACAATCCCAAGAATGCAAGTGATTGGAGGGCTCAGGTAGATCATGAAGACAGTGCTGTCATGGATCTTATCAGGTCCGATGATTGGGTGGTGCTGTTGGATGAGCGTGGGAAAGACATAGGTTCCATGCAGATGGCTGAGCTGATCGGAGATGCAGGGAATACGGGAGCTTCAAGACTATCATTCTGTGTTGGTGGACCTTATGGACATGGAAAACAATTGCGAGAGCGAGCTAACATATCTATCAAGTTGTCTTCTATGGTATTGAATCATCAAATTGCTATACTTGTGCTCGTAGAACAACTATATAGATCATGGACCATTCTGAAAGGTCAGAACTATCATCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SPOUT_MTase

Protein Analysis

217

Amino Acids

24.05

Weight (kDa)

9.28

Isoelectric Point (pI)

23.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPOUT_MTase PF02590 64 - 216 2.1e-44 Predicted SPOUT methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015123)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10620 AT5G10620
fragaria_vesca FvH4_2g30670
malus_domestica MD15G1110100.v1.1
prunus_persica Prupe.1G462300_v2.0.a1
pyrus_communis pycom15g10030
rosa_chinensis RchiOBHm_Chr6g0312221
rosa_laevigata RLG00000010341
rosa_multiflora Rmu_sc0010924.1_g000004
rosa_roxburghii Rroxscaffold_7G00156940
rosa_rugosa Rorug06G0394200
rosa_samantha Rh6AG511100 Rh6BG522100 Rh6CG527900 Rh6DG513300
rosa_wichuraiana Rw6G044450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 359
AclWI GGATC 1 cut(s) 375
AcuI CTGAAG 1 cut(s) 33
AdeI CACNNNGTG 1 cut(s) 230
AfaI GTAC 2 cut(s) 164, 234
AfiI CCNNNNNNNGG 1 cut(s) 387
AflII CTTAAG 1 cut(s) 266
AgsI TTSAA 3 cut(s) 260, 477, 571
AluBI AGCT 3 cut(s) 445, 473, 536
AluI AGCT 3 cut(s) 445, 473, 536
Alw21I GWGCWC 2 cut(s) 187, 597
AlwI GGATC 1 cut(s) 375
ApeKI GCWGC 1 cut(s) 77
AspS9I GGNCC 3 cut(s) 378, 500, 621
AvaII GGWCC 3 cut(s) 378, 500, 621
BanII GRGCYC 1 cut(s) 337
BbsI GAAGAC 2 cut(s) 357, 549
Bbv12I GWGCWC 2 cut(s) 187, 597
BbvI GCAGC 1 cut(s) 89
BccI CCATC 1 cut(s) 430
BfaI CTAG 2 cut(s) 279, 652
BfrI CTTAAG 1 cut(s) 266
BisI GCNGC 1 cut(s) 78
BlpI GCTNAGC 1 cut(s) 441
BlsI GCNGC 1 cut(s) 79
Bme18I GGWCC 3 cut(s) 378, 500, 621
BmgT120I GGNCC 3 cut(s) 378, 500, 621
BmiI GGNNCC 1 cut(s) 427
BmsI GCATC 1 cut(s) 445
BpiI GAAGAC 2 cut(s) 357, 549
Bpu10I CCTNAGC 1 cut(s) 336
Bpu1102I GCTNAGC 1 cut(s) 441
Bsc4I CCNNNNNNNGG 1 cut(s) 387
BseGI GGATG 3 cut(s) 253, 295, 409
BseLI CCNNNNNNNGG 1 cut(s) 387
BseMII CTCAG 2 cut(s) 350, 432
BseXI GCAGC 1 cut(s) 89
BsgI GTGCAG 1 cut(s) 24
BsiHKAI GWGCWC 2 cut(s) 187, 597
BslI CCNNNNNNNGG 1 cut(s) 387
BsmI GAATGC 1 cut(s) 322
Bsp1286I GDGCHC 3 cut(s) 187, 337, 597
Bsp1407I TGTACA 1 cut(s) 232
Bsp143I GATC 5 cut(s) 221, 343, 367, 447, 614
Bsp1720I GCTNAGC 1 cut(s) 441
BspCNI CTCAG 2 cut(s) 349, 433
BspHI TCATGA 1 cut(s) 346
BspLI GGNNCC 1 cut(s) 427
BspPI GGATC 1 cut(s) 375
BspTI CTTAAG 1 cut(s) 266
BsrGI TGTACA 1 cut(s) 232
BssMI GATC 5 cut(s) 221, 343, 367, 447, 614
Bst4CI ACNGT 2 cut(s) 59, 356
BstAFI CTTAAG 1 cut(s) 266
BstAPI GCANNNNNTGC 1 cut(s) 74
BstAUI TGTACA 1 cut(s) 232
BstC8I GCNNGC 2 cut(s) 70, 534
BstDEI CTNAG 3 cut(s) 14, 336, 441
BstF5I GGATG 3 cut(s) 253, 295, 409
BstKTI GATC 5 cut(s) 224, 346, 370, 450, 617
BstMBI GATC 5 cut(s) 221, 343, 367, 447, 614
BstMWI GCNNNNNNNGC 2 cut(s) 74, 182
BstV1I GCAGC 1 cut(s) 89
BstV2I GAAGAC 2 cut(s) 357, 549
BstX2I RGATCY 1 cut(s) 367
BstXI CCANNNNNNTGG 1 cut(s) 436
BstYI RGATCY 1 cut(s) 367
BtgZI GCGATG 1 cut(s) 632
BtsCI GGATG 3 cut(s) 253, 295, 409
BtsI GCAGTG 1 cut(s) 183
BtsIMutI CAGTG 2 cut(s) 183, 361
Cac8I GCNNGC 2 cut(s) 70, 534
CciI TCATGA 1 cut(s) 346
Cfr13I GGNCC 3 cut(s) 378, 500, 621
Csp6I GTAC 2 cut(s) 163, 233
CviAII CATG 5 cut(s) 347, 364, 430, 512, 618
CviJI RGCY 7 cut(s) 72, 265, 335, 440, 445, 473, 536
CviKI_1 RGCY 7 cut(s) 72, 265, 335, 440, 445, 473, 536
CviQI GTAC 2 cut(s) 163, 233
DdeI CTNAG 3 cut(s) 14, 336, 441
DpnI GATC 5 cut(s) 223, 345, 369, 449, 616
DpnII GATC 5 cut(s) 221, 343, 367, 447, 614
DraIII CACNNNGTG 1 cut(s) 230
DrdI GACNNNNNNGTC 1 cut(s) 359
DseDI GACNNNNNNGTC 1 cut(s) 359
Eco24I GRGCYC 1 cut(s) 337
Eco47I GGWCC 3 cut(s) 378, 500, 621
Eco57I CTGAAG 1 cut(s) 33
EcoT38I GRGCYC 1 cut(s) 337
FaeI CATG 5 cut(s) 350, 367, 433, 515, 621
FatI CATG 5 cut(s) 346, 363, 429, 511, 617
Fnu4HI GCNGC 1 cut(s) 78
FokI GGATG 3 cut(s) 260, 302, 416
FriOI GRGCYC 1 cut(s) 337
Fsp4HI GCNGC 1 cut(s) 78
FspBI CTAG 2 cut(s) 279, 652
GluI GCNGC 1 cut(s) 78
Hin1II CATG 5 cut(s) 350, 367, 433, 515, 621
HinfI GANTC 1 cut(s) 571
Hpy166II GTNNAC 4 cut(s) 165, 233, 295, 500
Hpy188I TCNGA 5 cut(s) 141, 382, 452, 630, 639
Hpy188III TCNNGA 2 cut(s) 347, 477
Hpy8I GTNNAC 4 cut(s) 165, 233, 295, 500
HpyAV CCTTC 2 cut(s) 57, 100
HpyCH4III ACNGT 2 cut(s) 59, 356
HpyCH4V TGCA 6 cut(s) 41, 77, 159, 320, 433, 458
HpyF10VI GCNNNNNNNGC 2 cut(s) 74, 182
HpyF3I CTNAG 3 cut(s) 14, 336, 441
Hsp92II CATG 5 cut(s) 350, 367, 433, 515, 621
Kzo9I GATC 5 cut(s) 221, 343, 367, 447, 614
LmnI GCTCC 1 cut(s) 470
LpnPI CCDG 6 cut(s) 36, 132, 153, 323, 361, 444
Lsp1109I GCAGC 1 cut(s) 89
LweI GCATC 1 cut(s) 445
MaeI CTAG 2 cut(s) 279, 652
MaeIII GTNAC 1 cut(s) 53
MalI GATC 5 cut(s) 223, 345, 369, 449, 616
MboI GATC 5 cut(s) 221, 343, 367, 447, 614
MboII GAAGA 2 cut(s) 362, 549
MfeI CAATTG 1 cut(s) 521
MflI RGATCY 1 cut(s) 367
MhlI GDGCHC 3 cut(s) 187, 337, 597
MluCI AATT 3 cut(s) 31, 521, 579
MmeI TCCRAC 1 cut(s) 381
MnlI CCTC 3 cut(s) 31, 280, 324
MseI TTAA 1 cut(s) 267
MslI CAYNNNNRTG 1 cut(s) 434
MspCI CTTAAG 1 cut(s) 266
MunI CAATTG 1 cut(s) 521
Mva1269I GAATGC 1 cut(s) 322
MwoI GCNNNNNNNGC 2 cut(s) 74, 182
NdeII GATC 5 cut(s) 221, 343, 367, 447, 614
NlaIII CATG 5 cut(s) 350, 367, 433, 515, 621
NlaIV GGNNCC 1 cut(s) 427
PagI TCATGA 1 cut(s) 346
PctI GAATGC 1 cut(s) 322
PfeI GAWTC 1 cut(s) 571
PkrI GCNGC 1 cut(s) 79
PspN4I GGNNCC 1 cut(s) 427
PspPI GGNCC 3 cut(s) 378, 500, 621
PsuI RGATCY 1 cut(s) 367
RsaI GTAC 2 cut(s) 164, 234
RsaNI GTAC 2 cut(s) 163, 233
RseI CAYNNNNRTG 1 cut(s) 434
SaqAI TTAA 1 cut(s) 267
SatI GCNGC 1 cut(s) 78
Sau3AI GATC 5 cut(s) 221, 343, 367, 447, 614
Sau96I GGNCC 3 cut(s) 378, 500, 621
SduI GDGCHC 3 cut(s) 187, 337, 597
SfaNI GCATC 1 cut(s) 445
SinI GGWCC 3 cut(s) 378, 500, 621
SmiMI CAYNNNNRTG 1 cut(s) 434
SmlI CTYRAG 1 cut(s) 266
SmoI CTYRAG 1 cut(s) 266
Sse9I AATT 3 cut(s) 31, 521, 579
SspMI CTAG 2 cut(s) 279, 652
TaaI ACNGT 2 cut(s) 59, 356
TasI AATT 3 cut(s) 31, 521, 579
TatI WGTACW 2 cut(s) 162, 232
TfiI GAWTC 1 cut(s) 571
Tru1I TTAA 1 cut(s) 267
Tru9I TTAA 1 cut(s) 267
TscAI CASTG 2 cut(s) 183, 361
TseI GCWGC 1 cut(s) 77
TspDTI ATGAA 1 cut(s) 363
TspRI CASTG 2 cut(s) 183, 361
Vha464I CTTAAG 1 cut(s) 266
VpaK11BI GGWCC 3 cut(s) 378, 500, 621
XspI CTAG 2 cut(s) 279, 652
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.