RLG00000010341

RNA methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0015123
Physical Location & Seq
Reverse (-)
823088 .. 824658
1571 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010341

Sequence Viewer

Length: 558 bp
ATGGTTGTGGTGCTTAGTGCCTCTGCTACCAATTTCTCTGCACTTCCTTCAGGTAAAGGTTGCAAGTACACAGGTCAAGCAGTGAGAGCACTTCCTATGCGCATAATATCGGTGGGGAAAAAGAGATCACAAGGTGTACAACTCGTTGTGGATGACTACATTCAAAGGCTCAAGTTATACTGTAGTGTTGAGGATGTTCACATTAAGAACAATCCTAAGAATGCAAGTGATTGGAGGGCTCAGGTTGATCATGAAGACAGTGCTGTCATGGATCTTATCAGGTCCGATGATTGGGTGGTGCTCTTGGATGAGCGTGGGAAAGACATAGGTTCCATGCAGATGGCTGAGCTGATTGGAGATGCAGGGAATATGGGAGCTTCAAGACTATCATTCTGTGTTGGTGGACCTTATGGACATGGAAAACAATTGCGAGAACGAGCTAACATATCTATCAAGTTGTCTTCTATGGTATTAAATCATCAAATTGCTATACTTGTGCTCGTAGAACAACTATATAGGTCATGGACCATTTTGAAAGGTCAGAACTATCATCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SPOUT_MTase

Protein Analysis

186

Amino Acids

20.53

Weight (kDa)

9.17

Isoelectric Point (pI)

23.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPOUT_MTase PF02590 33 - 184 7.8e-45 Predicted SPOUT methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015123)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10620 AT5G10620
fragaria_vesca FvH4_2g30670
malus_domestica MD15G1110100.v1.1
prunus_persica Prupe.1G462300_v2.0.a1
pyrus_communis pycom15g10030
rosa_chinensis RchiOBHm_Chr6g0312221
rosa_laevigata RLG00000010341
rosa_multiflora Rmu_sc0010924.1_g000004
rosa_roxburghii Rroxscaffold_7G00156940
rosa_rugosa Rorug06G0394200
rosa_samantha Rh6AG511100 Rh6BG522100 Rh6CG527900 Rh6DG513300
rosa_wichuraiana Rw6G044450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 263
Acc16I TGCGCA 1 cut(s) 101
AclWI GGATC 1 cut(s) 279
AcuI CTGAAG 1 cut(s) 33
AdeI CACNNNGTG 1 cut(s) 134
AfaI GTAC 2 cut(s) 68, 138
AfiI CCNNNNNNNGG 1 cut(s) 291
AgsI TTSAA 3 cut(s) 164, 381, 535
AluBI AGCT 3 cut(s) 349, 377, 440
AluI AGCT 3 cut(s) 349, 377, 440
Alw21I GWGCWC 3 cut(s) 91, 303, 501
AlwI GGATC 1 cut(s) 279
AspLEI GCGC 1 cut(s) 102
AspS9I GGNCC 3 cut(s) 282, 404, 525
AvaII GGWCC 3 cut(s) 282, 404, 525
BanII GRGCYC 1 cut(s) 241
BbsI GAAGAC 2 cut(s) 261, 453
Bbv12I GWGCWC 3 cut(s) 91, 303, 501
BccI CCATC 1 cut(s) 334
BclI TGATCA 1 cut(s) 247
BfaI CTAG 1 cut(s) 556
BfmI CTRYAG 1 cut(s) 181
BlpI GCTNAGC 1 cut(s) 345
Bme18I GGWCC 3 cut(s) 282, 404, 525
BmgT120I GGNCC 3 cut(s) 282, 404, 525
BmiI GGNNCC 1 cut(s) 331
BmsI GCATC 1 cut(s) 349
BpiI GAAGAC 2 cut(s) 261, 453
Bpu10I CCTNAGC 1 cut(s) 240
Bpu1102I GCTNAGC 1 cut(s) 345
BpuEI CTTGAG 1 cut(s) 155
Bsc4I CCNNNNNNNGG 1 cut(s) 291
BseGI GGATG 3 cut(s) 157, 199, 313
BseLI CCNNNNNNNGG 1 cut(s) 291
BseMII CTCAG 2 cut(s) 254, 336
BsgI GTGCAG 1 cut(s) 24
BsiHKAI GWGCWC 3 cut(s) 91, 303, 501
BslI CCNNNNNNNGG 1 cut(s) 291
BsmI GAATGC 1 cut(s) 226
Bsp1286I GDGCHC 4 cut(s) 91, 241, 303, 501
Bsp1407I TGTACA 1 cut(s) 136
Bsp143I GATC 3 cut(s) 125, 247, 271
Bsp1720I GCTNAGC 1 cut(s) 345
BspCNI CTCAG 2 cut(s) 253, 337
BspHI TCATGA 1 cut(s) 250
BspLI GGNNCC 1 cut(s) 331
BspPI GGATC 1 cut(s) 279
BsrGI TGTACA 1 cut(s) 136
BssMI GATC 3 cut(s) 125, 247, 271
Bst4CI ACNGT 2 cut(s) 182, 260
BstAUI TGTACA 1 cut(s) 136
BstDEI CTNAG 4 cut(s) 14, 216, 240, 345
BstF5I GGATG 3 cut(s) 157, 199, 313
BstHHI GCGC 1 cut(s) 102
BstKTI GATC 3 cut(s) 128, 250, 274
BstMBI GATC 3 cut(s) 125, 247, 271
BstMWI GCNNNNNNNGC 1 cut(s) 86
BstSFI CTRYAG 1 cut(s) 181
BstV2I GAAGAC 2 cut(s) 261, 453
BstX2I RGATCY 1 cut(s) 271
BstXI CCANNNNNNTGG 1 cut(s) 340
BstYI RGATCY 1 cut(s) 271
BtgZI GCGATG 1 cut(s) 536
BtsCI GGATG 3 cut(s) 157, 199, 313
BtsI GCAGTG 1 cut(s) 87
BtsIMutI CAGTG 2 cut(s) 87, 265
CciI TCATGA 1 cut(s) 250
CfoI GCGC 1 cut(s) 102
Cfr13I GGNCC 3 cut(s) 282, 404, 525
Csp6I GTAC 2 cut(s) 67, 137
CviAII CATG 5 cut(s) 251, 268, 334, 416, 522
CviJI RGCY 6 cut(s) 169, 239, 344, 349, 377, 440
CviKI_1 RGCY 6 cut(s) 169, 239, 344, 349, 377, 440
CviQI GTAC 2 cut(s) 67, 137
DdeI CTNAG 4 cut(s) 14, 216, 240, 345
DpnI GATC 3 cut(s) 127, 249, 273
DpnII GATC 3 cut(s) 125, 247, 271
DraIII CACNNNGTG 1 cut(s) 134
DrdI GACNNNNNNGTC 1 cut(s) 263
DseDI GACNNNNNNGTC 1 cut(s) 263
Eco24I GRGCYC 1 cut(s) 241
Eco47I GGWCC 3 cut(s) 282, 404, 525
Eco57I CTGAAG 1 cut(s) 33
EcoT38I GRGCYC 1 cut(s) 241
FaeI CATG 5 cut(s) 254, 271, 337, 419, 525
FatI CATG 5 cut(s) 250, 267, 333, 415, 521
FbaI TGATCA 1 cut(s) 247
FokI GGATG 3 cut(s) 164, 206, 320
FriOI GRGCYC 1 cut(s) 241
FspAI RTGCGCAY 1 cut(s) 101
FspBI CTAG 1 cut(s) 556
FspI TGCGCA 1 cut(s) 101
GlaI GCGC 1 cut(s) 101
HhaI GCGC 1 cut(s) 102
Hin1II CATG 5 cut(s) 254, 271, 337, 419, 525
Hin6I GCGC 1 cut(s) 100
HinP1I GCGC 1 cut(s) 100
Hpy166II GTNNAC 4 cut(s) 69, 137, 199, 404
Hpy188I TCNGA 2 cut(s) 286, 543
Hpy188III TCNNGA 2 cut(s) 251, 381
Hpy8I GTNNAC 4 cut(s) 69, 137, 199, 404
HpyAV CCTTC 1 cut(s) 57
HpyCH4III ACNGT 2 cut(s) 182, 260
HpyCH4V TGCA 5 cut(s) 41, 63, 224, 337, 362
HpyF10VI GCNNNNNNNGC 1 cut(s) 86
HpyF3I CTNAG 4 cut(s) 14, 216, 240, 345
Hsp92II CATG 5 cut(s) 254, 271, 337, 419, 525
HspAI GCGC 1 cut(s) 100
Ksp22I TGATCA 1 cut(s) 247
Kzo9I GATC 3 cut(s) 125, 247, 271
LmnI GCTCC 1 cut(s) 374
LpnPI CCDG 5 cut(s) 36, 57, 227, 265, 348
LweI GCATC 1 cut(s) 349
MaeI CTAG 1 cut(s) 556
MalI GATC 3 cut(s) 127, 249, 273
MboI GATC 3 cut(s) 125, 247, 271
MboII GAAGA 2 cut(s) 266, 453
MfeI CAATTG 1 cut(s) 425
MflI RGATCY 1 cut(s) 271
MhlI GDGCHC 4 cut(s) 91, 241, 303, 501
MluCI AATT 3 cut(s) 31, 425, 483
MnlI CCTC 3 cut(s) 31, 184, 228
MseI TTAA 2 cut(s) 204, 473
MslI CAYNNNNRTG 1 cut(s) 338
MunI CAATTG 1 cut(s) 425
Mva1269I GAATGC 1 cut(s) 226
MwoI GCNNNNNNNGC 1 cut(s) 86
NdeII GATC 3 cut(s) 125, 247, 271
NlaIII CATG 5 cut(s) 254, 271, 337, 419, 525
NlaIV GGNNCC 1 cut(s) 331
NsbI TGCGCA 1 cut(s) 101
PagI TCATGA 1 cut(s) 250
PctI GAATGC 1 cut(s) 226
PspN4I GGNNCC 1 cut(s) 331
PspPI GGNCC 3 cut(s) 282, 404, 525
PsuI RGATCY 1 cut(s) 271
RsaI GTAC 2 cut(s) 68, 138
RsaNI GTAC 2 cut(s) 67, 137
RseI CAYNNNNRTG 1 cut(s) 338
SaqAI TTAA 2 cut(s) 204, 473
Sau3AI GATC 3 cut(s) 125, 247, 271
Sau96I GGNCC 3 cut(s) 282, 404, 525
SduI GDGCHC 4 cut(s) 91, 241, 303, 501
SfaNI GCATC 1 cut(s) 349
SfcI CTRYAG 1 cut(s) 181
SinI GGWCC 3 cut(s) 282, 404, 525
SmiMI CAYNNNNRTG 1 cut(s) 338
SmlI CTYRAG 1 cut(s) 170
SmoI CTYRAG 1 cut(s) 170
Sse9I AATT 3 cut(s) 31, 425, 483
SspMI CTAG 1 cut(s) 556
TaaI ACNGT 2 cut(s) 182, 260
TasI AATT 3 cut(s) 31, 425, 483
TatI WGTACW 2 cut(s) 66, 136
Tru1I TTAA 2 cut(s) 204, 473
Tru9I TTAA 2 cut(s) 204, 473
TscAI CASTG 2 cut(s) 87, 265
TspDTI ATGAA 1 cut(s) 267
TspRI CASTG 2 cut(s) 87, 265
VpaK11BI GGWCC 3 cut(s) 282, 404, 525
XspI CTAG 1 cut(s) 556
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.