MD15G1110100.v1.1

RNA methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0015123
Physical Location & Seq
Forward (+)
7698301 .. 7701528
3228 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1110100.v1.1.491

Sequence Viewer

Length: 768 bp
ATGGGCCTCACTTCTTGTGCTCATTCCATTTATAAACTCCAACCCATCCAAAAAAAACCAAACAAACAAACAGAAGTGCTTCTCCTCCCCTGCTCGTTCTATCAGCAACCCAGAAACTCGATTCTGCGTCTTGTGCTTCGAAAGCCGTCTGTTGGCGAGCCTTTCTGGTGGGCACTGTCCGGTTGGATTTTTGAAGCTCAAGTTGAAATGGTTGTCATGCTTAGTGCCCGTCTCCACACCGTTACCTCTGCTCTTCCTTCAGGTAGAGATTGTAAATACACAGGTCAAGCAGTGAAAGCACTTCCTATACGCATAATATCAGTAGGGAAGAAGAGATCACAAGGAGTACAACTCGTAGTGGATGAATACATCGAAAAGCTTAAGCTCTACTGCAGCGTTGATGATGTTCACATACGGAACAATCCTAAAAATGCACATGATTCGAAGGCTCAGGTTGATCATGAAGACAGTGCTGTCATGGACCTTATAAGGTCCAATGATTGGGTCGTGCTGTTGGATGAGCGCGGGAAAGACATAGGGTCGGAGCAGATGGCAGAGTTGGTGGGGGATGCAGGGAATACAGGAGCTTCAAGACTATCCTTCTGCGTTGGTGGACCTTACGGCCATGGAAAACGATTGCGAGAACGTGCTGATGTTTCTATCAAGTTGTCTTCTATGGTTTTAAACCATCAAATTGCATTAGTTGTGCTTGTCGAACAACTCTATAGGGCATGGACTATTCTGAAAGGACAGAACTATCACCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SPOUT_MTase

Protein Analysis

256

Amino Acids

28.63

Weight (kDa)

9.25

Isoelectric Point (pI)

30.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPOUT_MTase PF02590 103 - 254 5.5e-46 Predicted SPOUT methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015123)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10620 AT5G10620
fragaria_vesca FvH4_2g30670
malus_domestica MD15G1110100.v1.1
prunus_persica Prupe.1G462300_v2.0.a1
pyrus_communis pycom15g10030
rosa_chinensis RchiOBHm_Chr6g0312221
rosa_laevigata RLG00000010341
rosa_multiflora Rmu_sc0010924.1_g000004
rosa_roxburghii Rroxscaffold_7G00156940
rosa_rugosa Rorug06G0394200
rosa_samantha Rh6AG511100 Rh6BG522100 Rh6CG527900 Rh6DG513300
rosa_wichuraiana Rw6G044450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 33, 488
AasI GACNNNNNNGTC 1 cut(s) 473
AccB7I CCANNNNNTGG 1 cut(s) 501
AccII CGCG 1 cut(s) 525
AciI CCGC 1 cut(s) 525
AcoI YGGCCR 1 cut(s) 622
AcuI CTGAAG 1 cut(s) 243
AfaI GTAC 1 cut(s) 348
AfiI CCNNNNNNNGG 2 cut(s) 152, 501
AflII CTTAAG 1 cut(s) 380
AgeI ACCGGT 1 cut(s) 762
AgsI TTSAA 3 cut(s) 194, 206, 591
AhdI GACNNNNNGTC 1 cut(s) 538
AluBI AGCT 4 cut(s) 197, 379, 385, 587
AluI AGCT 4 cut(s) 197, 379, 385, 587
Alw21I GWGCWC 1 cut(s) 22
Alw26I GTCTC 1 cut(s) 236
AoxI GGCC 2 cut(s) 4, 622
ApeKI GCWGC 1 cut(s) 393
AsiGI ACCGGT 1 cut(s) 762
Asp700I GAANNNNTTC 1 cut(s) 78
AspLEI GCGC 1 cut(s) 525
AspS9I GGNCC 4 cut(s) 4, 481, 492, 614
AsuHPI GGTGA 1 cut(s) 752
AsuII TTCGAA 2 cut(s) 139, 443
AvaII GGWCC 3 cut(s) 481, 492, 614
BaeGI GKGCMC 2 cut(s) 175, 229
BbsI GAAGAC 2 cut(s) 471, 663
Bbv12I GWGCWC 1 cut(s) 22
BbvI GCAGC 1 cut(s) 405
BccI CCATC 3 cut(s) 53, 544, 696
BceAI ACGGC 2 cut(s) 130, 637
BcgI CGANNNNNNTGC 2 cut(s) 423, 457
BclI TGATCA 1 cut(s) 457
BcoDI GTCTC 1 cut(s) 236
BfmI CTRYAG 2 cut(s) 391, 724
BfrI CTTAAG 1 cut(s) 380
BisI GCNGC 1 cut(s) 394
BlsI GCNGC 1 cut(s) 395
Bme18I GGWCC 3 cut(s) 481, 492, 614
BmeRI GACNNNNNGTC 1 cut(s) 538
BmgT120I GGNCC 4 cut(s) 4, 481, 492, 614
BmsI GCATC 1 cut(s) 559
BpiI GAAGAC 2 cut(s) 471, 663
BplI GAGNNNNNCTC 2 cut(s) 336, 368
Bpu10I CCTNAGC 1 cut(s) 450
Bpu14I TTCGAA 2 cut(s) 139, 443
BpuEI CTTGAG 1 cut(s) 183
BsaJI CCNNGG 1 cut(s) 625
BsaWI WCCGGW 2 cut(s) 179, 762
Bsc4I CCNNNNNNNGG 2 cut(s) 152, 501
Bse118I RCCGGY 1 cut(s) 762
BseDI CCNNGG 1 cut(s) 625
BseGI GGATG 4 cut(s) 45, 367, 523, 574
BseLI CCNNNNNNNGG 2 cut(s) 152, 501
BseMII CTCAG 1 cut(s) 464
BseRI GAGGAG 1 cut(s) 74
BseSI GKGCMC 2 cut(s) 175, 229
BseXI GCAGC 1 cut(s) 405
Bsh1236I CGCG 1 cut(s) 525
BshFI GGCC 2 cut(s) 6, 624
BshTI ACCGGT 1 cut(s) 762
BsiHKAI GWGCWC 1 cut(s) 22
BsiSI CCGG 2 cut(s) 180, 763
BslI CCNNNNNNNGG 2 cut(s) 152, 501
BsmAI GTCTC 1 cut(s) 236
BsmBI CGTCTC 1 cut(s) 236
BsnI GGCC 2 cut(s) 6, 624
Bsp119I TTCGAA 2 cut(s) 139, 443
Bsp1286I GDGCHC 3 cut(s) 22, 175, 229
Bsp143I GATC 2 cut(s) 335, 457
Bsp19I CCATGG 1 cut(s) 625
BspACI CCGC 1 cut(s) 525
BspANI GGCC 2 cut(s) 6, 624
BspCNI CTCAG 1 cut(s) 463
BspFNI CGCG 1 cut(s) 525
BspHI TCATGA 1 cut(s) 460
BspMAI CTGCAG 1 cut(s) 395
BspQI GCTCTTC 1 cut(s) 258
BspT104I TTCGAA 2 cut(s) 139, 443
BspTI CTTAAG 1 cut(s) 380
BsrFI RCCGGY 1 cut(s) 762
BssAI RCCGGY 1 cut(s) 762
BssECI CCNNGG 1 cut(s) 625
BssMI GATC 2 cut(s) 335, 457
BssT1I CCWWGG 1 cut(s) 625
Bst4CI ACNGT 3 cut(s) 177, 241, 470
Bst6I CTCTTC 2 cut(s) 258, 326
BstAFI CTTAAG 1 cut(s) 380
BstBI TTCGAA 2 cut(s) 139, 443
BstC8I GCNNGC 1 cut(s) 158
BstDEI CTNAG 2 cut(s) 221, 450
BstDSI CCRYGG 1 cut(s) 625
BstF5I GGATG 4 cut(s) 45, 367, 523, 574
BstFNI CGCG 1 cut(s) 525
BstHHI GCGC 1 cut(s) 525
BstKTI GATC 2 cut(s) 338, 460
BstMAI GTCTC 1 cut(s) 236
BstMBI GATC 2 cut(s) 335, 457
BstMWI GCNNNNNNNGC 3 cut(s) 133, 142, 296
BstSFI CTRYAG 2 cut(s) 391, 724
BstSLI GKGCMC 2 cut(s) 175, 229
BstUI CGCG 1 cut(s) 525
BstV1I GCAGC 1 cut(s) 405
BstV2I GAAGAC 2 cut(s) 471, 663
BsuRI GGCC 2 cut(s) 6, 624
BtgI CCRYGG 1 cut(s) 625
BtsCI GGATG 4 cut(s) 45, 367, 523, 574
BtsI GCAGTG 1 cut(s) 297
BtsIMutI CAGTG 3 cut(s) 173, 297, 475
Cac8I GCNNGC 1 cut(s) 158
CciI TCATGA 1 cut(s) 460
CfoI GCGC 1 cut(s) 525
Cfr10I RCCGGY 1 cut(s) 762
Cfr13I GGNCC 4 cut(s) 4, 481, 492, 614
CseI GACGC 1 cut(s) 116
Csp6I GTAC 1 cut(s) 347
CspAI ACCGGT 1 cut(s) 762
CviAII CATG 6 cut(s) 217, 437, 461, 478, 626, 732
CviJI RGCY 9 cut(s) 6, 145, 160, 197, 379, 385, 449, 587, 624
CviKI_1 RGCY 9 cut(s) 6, 145, 160, 197, 379, 385, 449, 587, 624
CviQI GTAC 1 cut(s) 347
DdeI CTNAG 2 cut(s) 221, 450
DpnI GATC 2 cut(s) 337, 459
DpnII GATC 2 cut(s) 335, 457
DraI TTTAAA 1 cut(s) 684
DrdI GACNNNNNNGTC 1 cut(s) 473
DriI GACNNNNNGTC 1 cut(s) 538
DseDI GACNNNNNNGTC 1 cut(s) 473
EaeI YGGCCR 1 cut(s) 622
Eam1104I CTCTTC 2 cut(s) 258, 326
Eam1105I GACNNNNNGTC 1 cut(s) 538
EarI CTCTTC 2 cut(s) 258, 326
Eco130I CCWWGG 1 cut(s) 625
Eco47I GGWCC 3 cut(s) 481, 492, 614
Eco57I CTGAAG 1 cut(s) 243
EcoT14I CCWWGG 1 cut(s) 625
ErhI CCWWGG 1 cut(s) 625
Esp3I CGTCTC 1 cut(s) 236
FaeI CATG 6 cut(s) 220, 440, 464, 481, 629, 735
FatI CATG 6 cut(s) 216, 436, 460, 477, 625, 731
FauI CCCGC 1 cut(s) 518
FbaI TGATCA 1 cut(s) 457
Fnu4HI GCNGC 1 cut(s) 394
FokI GGATG 4 cut(s) 32, 374, 530, 581
Fsp4HI GCNGC 1 cut(s) 394
GlaI GCGC 1 cut(s) 524
GluI GCNGC 1 cut(s) 394
HaeIII GGCC 2 cut(s) 6, 624
HapII CCGG 2 cut(s) 180, 763
HgaI GACGC 1 cut(s) 116
HhaI GCGC 1 cut(s) 525
Hin1II CATG 6 cut(s) 220, 440, 464, 481, 629, 735
Hin6I GCGC 1 cut(s) 523
HinP1I GCGC 1 cut(s) 523
HindIII AAGCTT 1 cut(s) 377
HinfI GANTC 2 cut(s) 121, 440
HpaII CCGG 2 cut(s) 180, 763
HphI GGTGA 1 cut(s) 752
Hpy166II GTNNAC 2 cut(s) 409, 614
Hpy188I TCNGA 2 cut(s) 544, 744
Hpy188III TCNNGA 2 cut(s) 461, 591
Hpy8I GTNNAC 2 cut(s) 409, 614
HpyAV CCTTC 3 cut(s) 267, 439, 610
HpyCH4III ACNGT 3 cut(s) 177, 241, 470
HpyCH4IV ACGT 1 cut(s) 646
HpyCH4V TGCA 4 cut(s) 393, 434, 572, 698
HpyF10VI GCNNNNNNNGC 3 cut(s) 133, 142, 296
HpyF3I CTNAG 2 cut(s) 221, 450
HpySE526I ACGT 1 cut(s) 646
Hsp92II CATG 6 cut(s) 220, 440, 464, 481, 629, 735
HspAI GCGC 1 cut(s) 523
Ksp22I TGATCA 1 cut(s) 457
Kzo9I GATC 2 cut(s) 335, 457
LguI GCTCTTC 1 cut(s) 258
LmnI GCTCC 2 cut(s) 544, 584
LpnPI CCDG 9 cut(s) 103, 124, 151, 193, 246, 267, 437, 558, 567
Lsp1109I GCAGC 1 cut(s) 405
LweI GCATC 1 cut(s) 559
MaeII ACGT 1 cut(s) 646
MaeIII GTNAC 1 cut(s) 241
MalI GATC 2 cut(s) 337, 459
MboI GATC 2 cut(s) 335, 457
MboII GAAGA 5 cut(s) 245, 340, 343, 476, 663
MhlI GDGCHC 3 cut(s) 22, 175, 229
MluCI AATT 1 cut(s) 693
MmeI TCCRAC 4 cut(s) 64, 164, 495, 522
MnlI CCTC 3 cut(s) 17, 95, 256
MroXI GAANNNNTTC 1 cut(s) 78
MseI TTAA 2 cut(s) 381, 683
MspCI CTTAAG 1 cut(s) 380
MspI CCGG 2 cut(s) 180, 763
MvnI CGCG 1 cut(s) 525
MwoI GCNNNNNNNGC 3 cut(s) 133, 142, 296
NcoI CCATGG 1 cut(s) 625
NdeII GATC 2 cut(s) 335, 457
NlaIII CATG 6 cut(s) 220, 440, 464, 481, 629, 735
NspV TTCGAA 2 cut(s) 139, 443
PagI TCATGA 1 cut(s) 460
PciSI GCTCTTC 1 cut(s) 258
PdmI GAANNNNTTC 1 cut(s) 78
PfeI GAWTC 2 cut(s) 121, 440
PflMI CCANNNNNTGG 1 cut(s) 501
PinAI ACCGGT 1 cut(s) 762
PkrI GCNGC 1 cut(s) 395
PsiI TTATAA 2 cut(s) 33, 488
PspPI GGNCC 4 cut(s) 4, 481, 492, 614
PstI CTGCAG 1 cut(s) 395
RsaI GTAC 1 cut(s) 348
RsaNI GTAC 1 cut(s) 347
SapI GCTCTTC 1 cut(s) 258
SaqAI TTAA 2 cut(s) 381, 683
SatI GCNGC 1 cut(s) 394
Sau3AI GATC 2 cut(s) 335, 457
Sau96I GGNCC 4 cut(s) 4, 481, 492, 614
SduI GDGCHC 3 cut(s) 22, 175, 229
SfaNI GCATC 1 cut(s) 559
SfcI CTRYAG 2 cut(s) 391, 724
SfuI TTCGAA 2 cut(s) 139, 443
SinI GGWCC 3 cut(s) 481, 492, 614
SmlI CTYRAG 2 cut(s) 198, 380
SmoI CTYRAG 2 cut(s) 198, 380
Sse9I AATT 1 cut(s) 693
SsiI CCGC 1 cut(s) 525
StyI CCWWGG 1 cut(s) 625
TaaI ACNGT 3 cut(s) 177, 241, 470
TaiI ACGT 1 cut(s) 649
TaqI TCGA 5 cut(s) 119, 139, 372, 443, 714
TasI AATT 1 cut(s) 693
TatI WGTACW 1 cut(s) 346
TfiI GAWTC 2 cut(s) 121, 440
Tru1I TTAA 2 cut(s) 381, 683
Tru9I TTAA 2 cut(s) 381, 683
TscAI CASTG 3 cut(s) 180, 297, 475
TseI GCWGC 1 cut(s) 393
TspDTI ATGAA 2 cut(s) 378, 477
TspGWI ACGGA 1 cut(s) 430
TspRI CASTG 3 cut(s) 180, 297, 475
Van91I CCANNNNNTGG 1 cut(s) 501
Vha464I CTTAAG 1 cut(s) 380
VpaK11BI GGWCC 3 cut(s) 481, 492, 614
XmnI GAANNNNTTC 1 cut(s) 78
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.