Rh6CG527900

RNA methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0015123
Physical Location & Seq
Forward (+)
68189353 .. 68196667
7315 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG527900.1

Sequence Viewer

Length: 417 bp
ATGGTTGTGGTGCTTAGTGCCTCTGCTACCAATTTCTCTGCACTTCCTTCAGGTAAAGGTTGCAAGTACACAGGTCAAGCAGTGAGAGCACTTCCTATACGCATAATATCGGTAGGGAAAAAGAGATCACAAGGTGTACAACTCGTTGTGGATGACTACATTCAAAGGCTTAAGTTATACTCTAGTGTTGAGGATGTTCACATAAAGAACAATCCCAAGAATGCAAGTGATTGGAGGGCTCAGGTAGATCATGAAGACAGTGCTGTCATGGATCTTATCAGGTCCGATGATTGGGTGGTGCTGTTGGATGAGCGTGGGAAAGACATAGGTTCCATGCAGATGGCTGAGCTGATCGGAGATGCAGGGAATACGGTTTTCATTTTCTTGTCTGCTACTTTCTTTGTCTCCTTCAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SPOUT_MTase

Protein Analysis

138

Amino Acids

15.19

Weight (kDa)

6.07

Isoelectric Point (pI)

20.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPOUT_MTase PF02590 32 - 123 5.7e-16 Predicted SPOUT methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015123)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10620 AT5G10620
fragaria_vesca FvH4_2g30670
malus_domestica MD15G1110100.v1.1
prunus_persica Prupe.1G462300_v2.0.a1
pyrus_communis pycom15g10030
rosa_chinensis RchiOBHm_Chr6g0312221
rosa_laevigata RLG00000010341
rosa_multiflora Rmu_sc0010924.1_g000004
rosa_roxburghii Rroxscaffold_7G00156940
rosa_rugosa Rorug06G0394200
rosa_samantha Rh6AG511100 Rh6BG522100 Rh6CG527900 Rh6DG513300
rosa_wichuraiana Rw6G044450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 263
AclWI GGATC 1 cut(s) 279
AcuI CTGAAG 1 cut(s) 33
AdeI CACNNNGTG 1 cut(s) 134
AfaI GTAC 2 cut(s) 68, 138
AfiI CCNNNNNNNGG 1 cut(s) 291
AflII CTTAAG 1 cut(s) 170
AgsI TTSAA 2 cut(s) 164, 412
AluBI AGCT 1 cut(s) 349
AluI AGCT 1 cut(s) 349
Alw21I GWGCWC 1 cut(s) 91
Alw26I GTCTC 1 cut(s) 409
AlwI GGATC 1 cut(s) 279
AspS9I GGNCC 1 cut(s) 282
AvaII GGWCC 1 cut(s) 282
BanII GRGCYC 1 cut(s) 241
BbsI GAAGAC 1 cut(s) 261
Bbv12I GWGCWC 1 cut(s) 91
BccI CCATC 1 cut(s) 334
BcoDI GTCTC 1 cut(s) 409
BfaI CTAG 1 cut(s) 183
BfrI CTTAAG 1 cut(s) 170
BlpI GCTNAGC 1 cut(s) 345
Bme18I GGWCC 1 cut(s) 282
BmgT120I GGNCC 1 cut(s) 282
BmiI GGNNCC 1 cut(s) 331
BmsI GCATC 1 cut(s) 349
BpiI GAAGAC 1 cut(s) 261
Bpu10I CCTNAGC 1 cut(s) 240
Bpu1102I GCTNAGC 1 cut(s) 345
Bsc4I CCNNNNNNNGG 1 cut(s) 291
BseGI GGATG 3 cut(s) 157, 199, 313
BseLI CCNNNNNNNGG 1 cut(s) 291
BseMII CTCAG 2 cut(s) 254, 336
BsgI GTGCAG 1 cut(s) 24
BsiHKAI GWGCWC 1 cut(s) 91
BslI CCNNNNNNNGG 1 cut(s) 291
BsmAI GTCTC 1 cut(s) 409
BsmI GAATGC 1 cut(s) 226
Bsp1286I GDGCHC 2 cut(s) 91, 241
Bsp1407I TGTACA 1 cut(s) 136
Bsp143I GATC 4 cut(s) 125, 247, 271, 351
Bsp1720I GCTNAGC 1 cut(s) 345
BspCNI CTCAG 2 cut(s) 253, 337
BspHI TCATGA 1 cut(s) 250
BspLI GGNNCC 1 cut(s) 331
BspPI GGATC 1 cut(s) 279
BspTI CTTAAG 1 cut(s) 170
BsrGI TGTACA 1 cut(s) 136
BssMI GATC 4 cut(s) 125, 247, 271, 351
Bst4CI ACNGT 2 cut(s) 260, 373
BstAFI CTTAAG 1 cut(s) 170
BstAUI TGTACA 1 cut(s) 136
BstDEI CTNAG 3 cut(s) 14, 240, 345
BstF5I GGATG 3 cut(s) 157, 199, 313
BstKTI GATC 4 cut(s) 128, 250, 274, 354
BstMAI GTCTC 1 cut(s) 409
BstMBI GATC 4 cut(s) 125, 247, 271, 351
BstMWI GCNNNNNNNGC 1 cut(s) 86
BstV2I GAAGAC 1 cut(s) 261
BstX2I RGATCY 1 cut(s) 271
BstXI CCANNNNNNTGG 1 cut(s) 340
BstYI RGATCY 1 cut(s) 271
BtsCI GGATG 3 cut(s) 157, 199, 313
BtsI GCAGTG 1 cut(s) 87
BtsIMutI CAGTG 2 cut(s) 87, 265
CciI TCATGA 1 cut(s) 250
Cfr13I GGNCC 1 cut(s) 282
Csp6I GTAC 2 cut(s) 67, 137
CviAII CATG 3 cut(s) 251, 268, 334
CviJI RGCY 4 cut(s) 169, 239, 344, 349
CviKI_1 RGCY 4 cut(s) 169, 239, 344, 349
CviQI GTAC 2 cut(s) 67, 137
DdeI CTNAG 3 cut(s) 14, 240, 345
DpnI GATC 4 cut(s) 127, 249, 273, 353
DpnII GATC 4 cut(s) 125, 247, 271, 351
DraIII CACNNNGTG 1 cut(s) 134
DrdI GACNNNNNNGTC 1 cut(s) 263
DseDI GACNNNNNNGTC 1 cut(s) 263
Eco24I GRGCYC 1 cut(s) 241
Eco47I GGWCC 1 cut(s) 282
Eco57I CTGAAG 1 cut(s) 33
EcoT38I GRGCYC 1 cut(s) 241
FaeI CATG 3 cut(s) 254, 271, 337
FaiI YATR 8 cut(s) 98, 104, 178, 203, 252, 269, 326, 335
FatI CATG 3 cut(s) 250, 267, 333
FokI GGATG 3 cut(s) 164, 206, 320
FriOI GRGCYC 1 cut(s) 241
FspBI CTAG 1 cut(s) 183
Hin1II CATG 3 cut(s) 254, 271, 337
Hpy166II GTNNAC 3 cut(s) 69, 137, 199
Hpy188I TCNGA 2 cut(s) 286, 356
Hpy188III TCNNGA 1 cut(s) 251
Hpy8I GTNNAC 3 cut(s) 69, 137, 199
HpyAV CCTTC 1 cut(s) 57
HpyCH4III ACNGT 2 cut(s) 260, 373
HpyCH4V TGCA 5 cut(s) 41, 63, 224, 337, 362
HpyF10VI GCNNNNNNNGC 1 cut(s) 86
HpyF3I CTNAG 3 cut(s) 14, 240, 345
Hsp92II CATG 3 cut(s) 254, 271, 337
Kzo9I GATC 4 cut(s) 125, 247, 271, 351
LpnPI CCDG 5 cut(s) 36, 57, 227, 265, 348
LweI GCATC 1 cut(s) 349
MaeI CTAG 1 cut(s) 183
MalI GATC 4 cut(s) 127, 249, 273, 353
MboI GATC 4 cut(s) 125, 247, 271, 351
MboII GAAGA 1 cut(s) 266
MflI RGATCY 1 cut(s) 271
MhlI GDGCHC 2 cut(s) 91, 241
MluCI AATT 2 cut(s) 31, 412
MmeI TCCRAC 1 cut(s) 285
MnlI CCTC 3 cut(s) 31, 184, 228
MseI TTAA 2 cut(s) 171, 415
MslI CAYNNNNRTG 1 cut(s) 338
MspCI CTTAAG 1 cut(s) 170
Mva1269I GAATGC 1 cut(s) 226
MwoI GCNNNNNNNGC 1 cut(s) 86
NdeII GATC 4 cut(s) 125, 247, 271, 351
NlaIII CATG 3 cut(s) 254, 271, 337
NlaIV GGNNCC 1 cut(s) 331
PagI TCATGA 1 cut(s) 250
PctI GAATGC 1 cut(s) 226
PspN4I GGNNCC 1 cut(s) 331
PspPI GGNCC 1 cut(s) 282
PsuI RGATCY 1 cut(s) 271
RsaI GTAC 2 cut(s) 68, 138
RsaNI GTAC 2 cut(s) 67, 137
RseI CAYNNNNRTG 1 cut(s) 338
SaqAI TTAA 2 cut(s) 171, 415
Sau3AI GATC 4 cut(s) 125, 247, 271, 351
Sau96I GGNCC 1 cut(s) 282
SduI GDGCHC 2 cut(s) 91, 241
SetI ASST 8 cut(s) 55, 61, 76, 136, 246, 284, 331, 351
SfaNI GCATC 1 cut(s) 349
SinI GGWCC 1 cut(s) 282
SmiMI CAYNNNNRTG 1 cut(s) 338
SmlI CTYRAG 1 cut(s) 170
SmoI CTYRAG 1 cut(s) 170
Sse9I AATT 2 cut(s) 31, 412
SspMI CTAG 1 cut(s) 183
TaaI ACNGT 2 cut(s) 260, 373
TasI AATT 2 cut(s) 31, 412
TatI WGTACW 2 cut(s) 66, 136
Tru1I TTAA 2 cut(s) 171, 415
Tru9I TTAA 2 cut(s) 171, 415
TscAI CASTG 2 cut(s) 87, 265
TspDTI ATGAA 2 cut(s) 267, 367
TspRI CASTG 2 cut(s) 87, 265
Vha464I CTTAAG 1 cut(s) 170
VpaK11BI GGWCC 1 cut(s) 282
XspI CTAG 1 cut(s) 183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.