AT5G10700

Peptidyl-tRNA hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0010251
Physical Location & Seq
Reverse (-)
3378053 .. 3378901
849 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G10700.1

Sequence Viewer

Length: 603 bp
ATGGCGACCGCCGTCCTCTCCGCAGCGTTTCGATTCCCGATAGTTACCCGCCGACTCTCTGCTCCGGCGCCGCGAATTAGGTTTTATCATTCTACGATCCGATTTGCTCATTTCCGAAGATTCACAACGGCTTCTCCTTCAATGAGCCAGCAGACTGGCGAAATCGATGCCGTCGTCGATGCTTCGTCGGCGGAGAAACCCGACGACGTCGTGGTGCAATACGTTGTGCTTCGACGAGATCTAATCGATTCGTGGCCACTTGGGAGCGTTGTAACACAAGGATGTCATGCGTCAGTGGCAGCGATCTGGTCGTTCAAAGACGATCCGGTCACGCTTCAGTACTGTGATCCGCAGCACATTGATTCTATGCATAAGGTGACTCTGGAAGTTAAAGGGGAAACGCAGATGATGAATTTGTCAGAGAAGCTGAAATTAGGAGGAATCTCTCATAAGCTATGGATGGAGCAGCCGGAGAATATTCCGACGTGTATTGCTACAAAGCCTTATCCTAAATCCCAAGTCTCTTCTTTCTTCAAAAAGCTCAAGCTTTGCAAAAACACGATACACAAAGCAAGTCAGAGATTTGGATATATGTACATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTH2

Protein Analysis

200

Amino Acids

22.59

Weight (kDa)

9.41

Isoelectric Point (pI)

53.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTH2 PF01981 72 - 184 3.8e-25 Peptidyl-tRNA hydrolase PTH2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010251)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10700 AT5G10700
fragaria_vesca FvH4_2g36680
malus_domestica MD08G1127800.v1.1 MD15G1107200.v1.1
prunus_persica Prupe.1G459500_v2.0.a1
pyrus_communis pycom08g10680 pycom15g09800
rosa_chinensis RchiOBHm_Chr1g0366021 RchiOBHm_Chr6g0305051
rosa_laevigata RLG00000010923
rosa_multiflora Rmu_sc0017427.1_g000001
rosa_roxburghii Rroxscaffold_7G00163200
rosa_rugosa Rorug06G0337000
rosa_samantha Rh1AG334900 Rh6AG448600 Rh6BG452300 Rh6CG462300 Rh6DG449200
rosa_wichuraiana Rw1G029710 Rw6G039080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 326
AatII GACGTC 1 cut(s) 210
AccB1I GGYRCC 1 cut(s) 67
AccII CGCG 1 cut(s) 73
AciI CCGC 6 cut(s) 9, 21, 49, 71, 191, 350
AclWI GGATC 3 cut(s) 91, 317, 341
AcoI YGGCCR 1 cut(s) 254
AcsI RAATTY 1 cut(s) 412
AcuI CTGAAG 1 cut(s) 320
AcyI GRCGYC 2 cut(s) 68, 207
AfaI GTAC 2 cut(s) 341, 596
AflIII ACRYGT 1 cut(s) 485
AgsI TTSAA 3 cut(s) 141, 316, 535
AjiI CACGTC 1 cut(s) 486
AluBI AGCT 4 cut(s) 427, 454, 541, 547
AluI AGCT 4 cut(s) 427, 454, 541, 547
Alw26I GTCTC 1 cut(s) 526
AlwI GGATC 3 cut(s) 91, 317, 341
AoxI GGCC 1 cut(s) 254
ApeKI GCWGC 4 cut(s) 23, 299, 352, 466
ApoI RAATTY 1 cut(s) 412
AspLEI GCGC 1 cut(s) 70
AsuHPI GGTGA 1 cut(s) 388
BalI TGGCCA 1 cut(s) 256
BanI GGYRCC 1 cut(s) 67
BbvI GCAGC 4 cut(s) 35, 311, 364, 478
BccI CCATC 1 cut(s) 454
BceAI ACGGC 2 cut(s) 144, 155
BcgI CGANNNNNNTGC 2 cut(s) 149, 183
BcoDI GTCTC 1 cut(s) 526
BfoI RGCGCY 1 cut(s) 71
BglII AGATCT 1 cut(s) 238
BisI GCNGC 5 cut(s) 24, 71, 300, 353, 467
BlsI GCNGC 5 cut(s) 25, 72, 301, 354, 468
BmcAI AGTACT 1 cut(s) 341
BmgBI CACGTC 1 cut(s) 486
BmiI GGNNCC 1 cut(s) 69
BmsI GCATC 2 cut(s) 157, 169
BoxI GACNNNNGTC 1 cut(s) 11
BpuEI CTTGAG 1 cut(s) 527
Bsa29I ATCGAT 2 cut(s) 165, 246
BsaHI GRCGYC 2 cut(s) 68, 207
BsaWI WCCGGW 1 cut(s) 325
BsaXI ACNNNNNCTCC 2 cut(s) 118, 148
Bse1I ACTGG 1 cut(s) 160
BseCI ATCGAT 2 cut(s) 165, 246
BseGI GGATG 2 cut(s) 287, 465
BseNI ACTGG 1 cut(s) 160
BseXI GCAGC 4 cut(s) 35, 311, 364, 478
Bsh1236I CGCG 1 cut(s) 73
Bsh1285I CGRYCG 1 cut(s) 9
BshFI GGCC 1 cut(s) 256
BshNI GGYRCC 1 cut(s) 67
BshVI ATCGAT 2 cut(s) 165, 246
BsiEI CGRYCG 1 cut(s) 9
BsiSI CCGG 3 cut(s) 65, 326, 470
BsmAI GTCTC 1 cut(s) 526
BsnI GGCC 1 cut(s) 256
Bsp1407I TGTACA 1 cut(s) 594
Bsp143I GATC 5 cut(s) 96, 238, 303, 322, 346
BspACI CCGC 6 cut(s) 9, 21, 49, 71, 191, 350
BspANI GGCC 1 cut(s) 256
BspDI ATCGAT 2 cut(s) 165, 246
BspFNI CGCG 1 cut(s) 73
BspLI GGNNCC 1 cut(s) 69
BspPI GGATC 3 cut(s) 91, 317, 341
BspT107I GGYRCC 1 cut(s) 67
BsrGI TGTACA 1 cut(s) 594
BsrI ACTGG 1 cut(s) 160
BssMI GATC 5 cut(s) 96, 238, 303, 322, 346
BssNI GRCGYC 2 cut(s) 68, 207
Bst4CI ACNGT 1 cut(s) 344
Bst6I CTCTTC 1 cut(s) 529
BstACI GRCGYC 2 cut(s) 68, 207
BstAUI TGTACA 1 cut(s) 594
BstC8I GCNNGC 1 cut(s) 149
BstF5I GGATG 2 cut(s) 287, 465
BstFNI CGCG 1 cut(s) 73
BstH2I RGCGCY 1 cut(s) 71
BstHHI GCGC 1 cut(s) 70
BstKTI GATC 5 cut(s) 99, 241, 306, 325, 349
BstMAI GTCTC 1 cut(s) 526
BstMBI GATC 5 cut(s) 96, 238, 303, 322, 346
BstMCI CGRYCG 1 cut(s) 9
BstMWI GCNNNNNNNGC 2 cut(s) 188, 296
BstPAI GACNNNNGTC 1 cut(s) 11
BstUI CGCG 1 cut(s) 73
BstV1I GCAGC 4 cut(s) 35, 311, 364, 478
BstX2I RGATCY 1 cut(s) 238
BstXI CCANNNNNNTGG 1 cut(s) 155
BstYI RGATCY 1 cut(s) 238
Bsu15I ATCGAT 2 cut(s) 165, 246
BsuRI GGCC 1 cut(s) 256
BsuTUI ATCGAT 2 cut(s) 165, 246
BtrI CACGTC 1 cut(s) 486
BtsCI GGATG 2 cut(s) 287, 465
BtsIMutI CAGTG 1 cut(s) 300
Cac8I GCNNGC 1 cut(s) 149
CfoI GCGC 1 cut(s) 70
ClaI ATCGAT 2 cut(s) 165, 246
CseI GACGC 1 cut(s) 279
Csp6I GTAC 2 cut(s) 340, 595
CviAII CATG 1 cut(s) 287
CviJI RGCY 9 cut(s) 131, 147, 256, 427, 454, 469, 502, 541, 547
CviKI_1 RGCY 9 cut(s) 131, 147, 256, 427, 454, 469, 502, 541, 547
CviQI GTAC 2 cut(s) 340, 595
DinI GGCGCC 1 cut(s) 69
DpnI GATC 5 cut(s) 98, 240, 305, 324, 348
DpnII GATC 5 cut(s) 96, 238, 303, 322, 346
DrdI GACNNNNNNGTC 1 cut(s) 326
DseDI GACNNNNNNGTC 1 cut(s) 326
EaeI YGGCCR 1 cut(s) 254
Eam1104I CTCTTC 1 cut(s) 529
EarI CTCTTC 1 cut(s) 529
EciI GGCGGA 1 cut(s) 206
Eco57I CTGAAG 1 cut(s) 320
EcoT22I ATGCAT 1 cut(s) 372
EgeI GGCGCC 1 cut(s) 69
EheI GGCGCC 1 cut(s) 69
FaeI CATG 1 cut(s) 290
FaiI YATR 9 cut(s) 288, 368, 372, 450, 457, 591, 593, 599, 601
FatI CATG 1 cut(s) 286
FauI CCCGC 1 cut(s) 56
Fnu4HI GCNGC 5 cut(s) 24, 71, 300, 353, 467
FokI GGATG 2 cut(s) 294, 472
Fsp4HI GCNGC 5 cut(s) 24, 71, 300, 353, 467
GlaI GCGC 1 cut(s) 69
GluI GCNGC 5 cut(s) 24, 71, 300, 353, 467
HaeII RGCGCY 1 cut(s) 71
HaeIII GGCC 1 cut(s) 256
HapII CCGG 3 cut(s) 65, 326, 470
HgaI GACGC 1 cut(s) 279
HhaI GCGC 1 cut(s) 70
Hin1I GRCGYC 2 cut(s) 68, 207
Hin1II CATG 1 cut(s) 290
Hin6I GCGC 1 cut(s) 68
HinP1I GCGC 1 cut(s) 68
HindIII AAGCTT 1 cut(s) 545
HinfI GANTC 7 cut(s) 33, 54, 120, 248, 362, 379, 441
HpaII CCGG 3 cut(s) 65, 326, 470
HphI GGTGA 1 cut(s) 388
Hpy188I TCNGA 5 cut(s) 101, 116, 421, 483, 579
Hpy188III TCNNGA 2 cut(s) 37, 383
Hpy99I CGWCG 8 cut(s) 176, 179, 190, 206, 209, 212, 237, 487
HpyAV CCTTC 1 cut(s) 147
HpyCH4III ACNGT 1 cut(s) 344
HpyCH4IV ACGT 3 cut(s) 207, 222, 485
HpyCH4V TGCA 3 cut(s) 217, 370, 552
HpyF10VI GCNNNNNNNGC 2 cut(s) 188, 296
HpySE526I ACGT 3 cut(s) 207, 222, 485
Hsp92I GRCGYC 2 cut(s) 68, 207
Hsp92II CATG 1 cut(s) 290
HspAI GCGC 1 cut(s) 68
KasI GGCGCC 1 cut(s) 67
Kzo9I GATC 5 cut(s) 96, 238, 303, 322, 346
LmnI GCTCC 3 cut(s) 67, 264, 463
LpnPI CCDG 7 cut(s) 78, 141, 161, 292, 339, 368, 483
Lsp1109I GCAGC 4 cut(s) 35, 311, 364, 478
LweI GCATC 2 cut(s) 157, 169
MaeII ACGT 3 cut(s) 207, 222, 485
MaeIII GTNAC 4 cut(s) 43, 271, 328, 376
MalI GATC 5 cut(s) 98, 240, 305, 324, 348
MboI GATC 5 cut(s) 96, 238, 303, 322, 346
MboII GAAGA 3 cut(s) 129, 516, 523
MflI RGATCY 1 cut(s) 238
MlsI TGGCCA 1 cut(s) 256
MluCI AATT 3 cut(s) 75, 412, 431
MluNI TGGCCA 1 cut(s) 256
Mly113I GGCGCC 1 cut(s) 68
MlyI GAGTC 2 cut(s) 48, 373
MmeI TCCRAC 1 cut(s) 506
MnlI CCTC 2 cut(s) 26, 431
Mox20I TGGCCA 1 cut(s) 256
Mph1103I ATGCAT 1 cut(s) 372
MscI TGGCCA 1 cut(s) 256
MseI TTAA 1 cut(s) 390
MslI CAYNNNNRTG 1 cut(s) 280
Msp20I TGGCCA 1 cut(s) 256
MspI CCGG 3 cut(s) 65, 326, 470
MvnI CGCG 1 cut(s) 73
MwoI GCNNNNNNNGC 2 cut(s) 188, 296
NarI GGCGCC 1 cut(s) 68
NdeII GATC 5 cut(s) 96, 238, 303, 322, 346
NlaIII CATG 1 cut(s) 290
NlaIV GGNNCC 1 cut(s) 69
NmuCI GTSAC 2 cut(s) 328, 376
NsiI ATGCAT 1 cut(s) 372
PcsI WCGNNNNNNNCGW 1 cut(s) 171
PfeI GAWTC 5 cut(s) 33, 120, 248, 362, 441
PflFI GACNNNGTC 1 cut(s) 206
PkrI GCNGC 5 cut(s) 25, 72, 301, 354, 468
PleI GAGTC 2 cut(s) 48, 373
PluTI GGCGCC 1 cut(s) 71
PpsI GAGTC 2 cut(s) 48, 373
PshAI GACNNNNGTC 1 cut(s) 11
PspN4I GGNNCC 1 cut(s) 69
PsuI RGATCY 1 cut(s) 238
PsyI GACNNNGTC 1 cut(s) 206
RsaI GTAC 2 cut(s) 341, 596
RsaNI GTAC 2 cut(s) 340, 595
RseI CAYNNNNRTG 1 cut(s) 280
SaqAI TTAA 1 cut(s) 390
SatI GCNGC 5 cut(s) 24, 71, 300, 353, 467
Sau3AI GATC 5 cut(s) 96, 238, 303, 322, 346
ScaI AGTACT 1 cut(s) 341
SchI GAGTC 2 cut(s) 48, 373
SetI ASST 9 cut(s) 83, 210, 225, 378, 429, 456, 488, 543, 549
SfaNI GCATC 2 cut(s) 157, 169
SfoI GGCGCC 1 cut(s) 69
SmiMI CAYNNNNRTG 1 cut(s) 280
SmlI CTYRAG 1 cut(s) 542
SmoI CTYRAG 1 cut(s) 542
Sse9I AATT 3 cut(s) 75, 412, 431
SsiI CCGC 6 cut(s) 9, 21, 49, 71, 191, 350
SspDI GGCGCC 1 cut(s) 67
SspI AATATT 1 cut(s) 478
TaaI ACNGT 1 cut(s) 344
TaiI ACGT 3 cut(s) 210, 225, 488
TaqI TCGA 5 cut(s) 31, 165, 177, 232, 246
TasI AATT 3 cut(s) 75, 412, 431
TatI WGTACW 2 cut(s) 339, 594
TauI GCSGC 1 cut(s) 73
TfiI GAWTC 5 cut(s) 33, 120, 248, 362, 441
Tru1I TTAA 1 cut(s) 390
Tru9I TTAA 1 cut(s) 390
TscAI CASTG 1 cut(s) 300
TseFI GTSAC 2 cut(s) 328, 376
TseI GCWGC 4 cut(s) 23, 299, 352, 466
Tsp45I GTSAC 2 cut(s) 328, 376
TspDTI ATGAA 1 cut(s) 425
TspRI CASTG 1 cut(s) 300
Tth111I GACNNNGTC 1 cut(s) 206
XapI RAATTY 1 cut(s) 412
ZraI GACGTC 1 cut(s) 208
ZrmI AGTACT 1 cut(s) 341
Zsp2I ATGCAT 1 cut(s) 372
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.