MD08G1127800.v1.1

peptidyl-tRNA hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0010251
Physical Location & Seq
Forward (+)
12010684 .. 12012970
2287 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1127800.v1.1.491

Sequence Viewer

Length: 531 bp
ATGGCGTGTATACTCGCGCCACTTCGTCCCACCTCACTTTCACTTTCAGTCCCTCGAATCAGATTTGGGGCCAAACCTCCATCCAACGCGGCCCCCGCCCCGATTCGACTCATCCGAAATTCGATGAGTCAACCAGCCGCCGAGGCCAATCCGGACATCCCAAAATCCGACACCACGGACGTCCTGGTTCAGTACGTCGTGCTCCGGCGAGACCTAATCGACACGTGGCCGACGGGCAGCATGGTCACACAGGGCTGCCATGCCTCCGTATCCGCCATTTGGTCCCACAAAGACGATCCCTTCACCCTCCAGTACTGCAGCCCAGAAAACATCGATTCTATGCATAAGGTTACCCTTGAGGTGAAGGGAGAACCCCAGATAGTGAATTTGTCAGAGAAGCTCAAAGCAGGTGGCATAGCACACAAGCTCTGGATTGAACAACCCGAAAACTTCCCAACTTGTCTCGCCACAAAGCCGTACCCGAAATCCGTGGTATCCGCATATTTTAAAAAGTTGAAACTCTGTAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTH2

Protein Analysis

177

Amino Acids

19.38

Weight (kDa)

9.13

Isoelectric Point (pI)

52.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTH2 PF01981 63 - 175 8.3e-24 Peptidyl-tRNA hydrolase PTH2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010251)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10700 AT5G10700
fragaria_vesca FvH4_2g36680
malus_domestica MD08G1127800.v1.1 MD15G1107200.v1.1
prunus_persica Prupe.1G459500_v2.0.a1
pyrus_communis pycom08g10680 pycom15g09800
rosa_chinensis RchiOBHm_Chr1g0366021 RchiOBHm_Chr6g0305051
rosa_laevigata RLG00000010923
rosa_multiflora Rmu_sc0017427.1_g000001
rosa_roxburghii Rroxscaffold_7G00163200
rosa_rugosa Rorug06G0337000
rosa_samantha Rh1AG334900 Rh6AG448600 Rh6BG452300 Rh6CG462300 Rh6DG449200
rosa_wichuraiana Rw1G029710 Rw6G039080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 398
AatII GACGTC 1 cut(s) 183
Acc36I ACCTGC 1 cut(s) 398
AccI GTMKAC 1 cut(s) 10
AccII CGCG 2 cut(s) 17, 89
AccIII TCCGGA 1 cut(s) 151
AciI CCGC 5 cut(s) 89, 96, 138, 273, 498
AclWI GGATC 1 cut(s) 290
AcoI YGGCCR 1 cut(s) 227
AcsI RAATTY 2 cut(s) 118, 385
AcvI CACGTG 1 cut(s) 225
AcyI GRCGYC 1 cut(s) 180
AfaI GTAC 3 cut(s) 194, 314, 479
AfiI CCNNNNNNNGG 1 cut(s) 279
AflIII ACRYGT 1 cut(s) 222
AgsI TTSAA 2 cut(s) 437, 517
AjnI CCWGG 1 cut(s) 183
AluBI AGCT 2 cut(s) 400, 427
AluI AGCT 2 cut(s) 400, 427
Alw21I GWGCWC 1 cut(s) 204
Alw26I GTCTC 2 cut(s) 204, 467
AlwI GGATC 1 cut(s) 290
Aor13HI TCCGGA 1 cut(s) 151
AoxI GGCC 4 cut(s) 69, 90, 144, 227
ApeKI GCWGC 3 cut(s) 237, 255, 318
ApoI RAATTY 2 cut(s) 118, 385
AspLEI GCGC 1 cut(s) 19
AspS9I GGNCC 3 cut(s) 69, 91, 282
AsuHPI GGTGA 2 cut(s) 295, 373
AvaII GGWCC 1 cut(s) 282
BbrPI CACGTG 1 cut(s) 225
Bbv12I GWGCWC 1 cut(s) 204
BbvI GCAGC 3 cut(s) 242, 249, 330
BccI CCATC 1 cut(s) 88
BceAI ACGGC 1 cut(s) 460
BciT130I CCWGG 1 cut(s) 185
BciVI GTATCC 2 cut(s) 280, 505
BcoDI GTCTC 2 cut(s) 204, 467
BfmI CTRYAG 1 cut(s) 316
BfuAI ACCTGC 1 cut(s) 398
BfuI GTATCC 2 cut(s) 280, 505
BglI GCCNNNNNGGC 1 cut(s) 143
BisI GCNGC 5 cut(s) 90, 138, 238, 256, 319
BlsI GCNGC 5 cut(s) 91, 139, 239, 257, 320
BmcAI AGTACT 1 cut(s) 314
Bme1390I CCNGG 1 cut(s) 185
Bme18I GGWCC 1 cut(s) 282
BmgT120I GGNCC 3 cut(s) 69, 91, 282
BmiI GGNNCC 3 cut(s) 70, 93, 284
BmrFI CCNGG 1 cut(s) 185
BpmI CTGGAG 1 cut(s) 293
BpuEI CTTGAG 1 cut(s) 377
Bsa29I ATCGAT 1 cut(s) 333
BsaAI YACGTR 1 cut(s) 225
BsaHI GRCGYC 1 cut(s) 180
BsaI GGTCTC 1 cut(s) 204
BsaJI CCNNGG 3 cut(s) 141, 174, 489
BsaWI WCCGGW 1 cut(s) 151
Bsc4I CCNNNNNNNGG 1 cut(s) 279
Bse1I ACTGG 1 cut(s) 310
BseAI TCCGGA 1 cut(s) 151
BseBI CCWGG 1 cut(s) 185
BseCI ATCGAT 1 cut(s) 333
BseDI CCNNGG 3 cut(s) 141, 174, 489
BseGI GGATG 3 cut(s) 80, 111, 156
BseLI CCNNNNNNNGG 1 cut(s) 279
BseNI ACTGG 1 cut(s) 310
BseXI GCAGC 3 cut(s) 242, 249, 330
Bsh1236I CGCG 2 cut(s) 17, 89
BshFI GGCC 4 cut(s) 71, 92, 146, 229
BshVI ATCGAT 1 cut(s) 333
BsiHKAI GWGCWC 1 cut(s) 204
BsiSI CCGG 2 cut(s) 152, 205
BslFI GGGAC 3 cut(s) 12, 35, 268
BslI CCNNNNNNNGG 1 cut(s) 279
BsmAI GTCTC 2 cut(s) 204, 467
BsmFI GGGAC 3 cut(s) 12, 35, 268
BsnI GGCC 4 cut(s) 71, 92, 146, 229
Bso31I GGTCTC 1 cut(s) 204
Bsp1286I GDGCHC 1 cut(s) 204
Bsp13I TCCGGA 1 cut(s) 151
Bsp143I GATC 1 cut(s) 295
BspACI CCGC 5 cut(s) 89, 96, 138, 273, 498
BspANI GGCC 4 cut(s) 71, 92, 146, 229
BspDI ATCGAT 1 cut(s) 333
BspEI TCCGGA 1 cut(s) 151
BspFNI CGCG 2 cut(s) 17, 89
BspLI GGNNCC 3 cut(s) 70, 93, 284
BspMAI CTGCAG 1 cut(s) 320
BspMI ACCTGC 1 cut(s) 398
BspPI GGATC 1 cut(s) 290
BspTNI GGTCTC 1 cut(s) 204
BsrI ACTGG 1 cut(s) 310
BssECI CCNNGG 3 cut(s) 141, 174, 489
BssMI GATC 1 cut(s) 295
BssNAI GTATAC 1 cut(s) 11
BssNI GRCGYC 1 cut(s) 180
Bst1107I GTATAC 1 cut(s) 11
Bst2UI CCWGG 1 cut(s) 185
BstACI GRCGYC 1 cut(s) 180
BstBAI YACGTR 1 cut(s) 225
BstDSI CCRYGG 2 cut(s) 174, 489
BstEII GGTNACC 1 cut(s) 349
BstF5I GGATG 3 cut(s) 80, 111, 156
BstFNI CGCG 2 cut(s) 17, 89
BstHHI GCGC 1 cut(s) 19
BstKTI GATC 1 cut(s) 298
BstMAI GTCTC 2 cut(s) 204, 467
BstMBI GATC 1 cut(s) 295
BstMWI GCNNNNNNNGC 2 cut(s) 95, 143
BstNI CCWGG 1 cut(s) 185
BstPI GGTNACC 1 cut(s) 349
BstSCI CCNGG 1 cut(s) 183
BstSFI CTRYAG 1 cut(s) 316
BstUI CGCG 2 cut(s) 17, 89
BstV1I GCAGC 3 cut(s) 242, 249, 330
BstZ17I GTATAC 1 cut(s) 11
Bsu15I ATCGAT 1 cut(s) 333
BsuI GTATCC 2 cut(s) 280, 505
BsuRI GGCC 4 cut(s) 71, 92, 146, 229
BsuTUI ATCGAT 1 cut(s) 333
BtgI CCRYGG 2 cut(s) 174, 489
BtsCI GGATG 3 cut(s) 80, 111, 156
BveI ACCTGC 1 cut(s) 398
CfoI GCGC 1 cut(s) 19
Cfr13I GGNCC 3 cut(s) 69, 91, 282
ClaI ATCGAT 1 cut(s) 333
Csp6I GTAC 3 cut(s) 193, 313, 478
CspCI CAANNNNNGTGG 2 cut(s) 391, 426
CviAII CATG 2 cut(s) 241, 260
CviQI GTAC 3 cut(s) 193, 313, 478
DpnI GATC 1 cut(s) 297
DpnII GATC 1 cut(s) 295
DraI TTTAAA 1 cut(s) 508
EaeI YGGCCR 1 cut(s) 227
EciI GGCGGA 1 cut(s) 262
Eco31I GGTCTC 1 cut(s) 204
Eco47I GGWCC 1 cut(s) 282
Eco72I CACGTG 1 cut(s) 225
Eco91I GGTNACC 1 cut(s) 349
EcoO65I GGTNACC 1 cut(s) 349
EcoRII CCWGG 1 cut(s) 183
EcoT22I ATGCAT 1 cut(s) 345
FaeI CATG 2 cut(s) 244, 263
FaiI YATR 7 cut(s) 11, 242, 261, 341, 345, 416, 502
FaqI GGGAC 3 cut(s) 12, 35, 268
FatI CATG 2 cut(s) 240, 259
FauI CCCGC 1 cut(s) 103
FblI GTMKAC 1 cut(s) 10
Fnu4HI GCNGC 5 cut(s) 90, 138, 238, 256, 319
FokI GGATG 3 cut(s) 67, 98, 143
Fsp4HI GCNGC 5 cut(s) 90, 138, 238, 256, 319
GlaI GCGC 1 cut(s) 18
GluI GCNGC 5 cut(s) 90, 138, 238, 256, 319
GsuI CTGGAG 1 cut(s) 293
HaeIII GGCC 4 cut(s) 71, 92, 146, 229
HapII CCGG 2 cut(s) 152, 205
HhaI GCGC 1 cut(s) 19
Hin1I GRCGYC 1 cut(s) 180
Hin1II CATG 2 cut(s) 244, 263
Hin6I GCGC 1 cut(s) 17
HinP1I GCGC 1 cut(s) 17
HincII GTYRAC 1 cut(s) 131
HindII GTYRAC 1 cut(s) 131
HinfI GANTC 5 cut(s) 57, 103, 108, 127, 335
HpaII CCGG 2 cut(s) 152, 205
HphI GGTGA 2 cut(s) 295, 373
Hpy166II GTNNAC 2 cut(s) 11, 131
Hpy188I TCNGA 4 cut(s) 62, 116, 169, 394
Hpy188III TCNNGA 2 cut(s) 152, 430
Hpy8I GTNNAC 2 cut(s) 11, 131
Hpy99I CGWCG 2 cut(s) 200, 235
HpyAV CCTTC 2 cut(s) 310, 358
HpyCH4IV ACGT 3 cut(s) 180, 195, 224
HpyCH4V TGCA 2 cut(s) 318, 343
HpyF10VI GCNNNNNNNGC 2 cut(s) 95, 143
HpySE526I ACGT 3 cut(s) 180, 195, 224
Hsp92I GRCGYC 1 cut(s) 180
Hsp92II CATG 2 cut(s) 244, 263
HspAI GCGC 1 cut(s) 17
Kpn2I TCCGGA 1 cut(s) 151
Kzo9I GATC 1 cut(s) 295
LmnI GCTCC 1 cut(s) 207
Lsp1109I GCAGC 3 cut(s) 242, 249, 330
MaeII ACGT 3 cut(s) 180, 195, 224
MaeIII GTNAC 2 cut(s) 244, 349
MalI GATC 1 cut(s) 297
MboI GATC 1 cut(s) 295
MhlI GDGCHC 1 cut(s) 204
MluCI AATT 2 cut(s) 118, 385
MlyI GAGTC 2 cut(s) 102, 136
MmeI TCCRAC 2 cut(s) 108, 192
MnlI CCTC 7 cut(s) 43, 63, 87, 136, 274, 317, 352
Mph1103I ATGCAT 1 cut(s) 345
MroI TCCGGA 1 cut(s) 151
MseI TTAA 1 cut(s) 507
MspI CCGG 2 cut(s) 152, 205
MspR9I CCNGG 1 cut(s) 185
MvaI CCWGG 1 cut(s) 185
MvnI CGCG 2 cut(s) 17, 89
MwoI GCNNNNNNNGC 2 cut(s) 95, 143
NdeII GATC 1 cut(s) 295
NlaIII CATG 2 cut(s) 244, 263
NlaIV GGNNCC 3 cut(s) 70, 93, 284
NmeAIII GCCGAG 1 cut(s) 166
NmuCI GTSAC 1 cut(s) 244
NsiI ATGCAT 1 cut(s) 345
PaqCI CACCTGC 1 cut(s) 398
PcsI WCGNNNNNNNCGW 1 cut(s) 112
PfeI GAWTC 3 cut(s) 57, 103, 335
PkrI GCNGC 5 cut(s) 91, 139, 239, 257, 320
PleI GAGTC 2 cut(s) 102, 135
PmaCI CACGTG 1 cut(s) 225
PmlI CACGTG 1 cut(s) 225
PpsI GAGTC 2 cut(s) 102, 135
Ppu21I YACGTR 1 cut(s) 225
Psp6I CCWGG 1 cut(s) 183
PspCI CACGTG 1 cut(s) 225
PspEI GGTNACC 1 cut(s) 349
PspGI CCWGG 1 cut(s) 183
PspN4I GGNNCC 3 cut(s) 70, 93, 284
PspPI GGNCC 3 cut(s) 69, 91, 282
PstI CTGCAG 1 cut(s) 320
RsaI GTAC 3 cut(s) 194, 314, 479
RsaNI GTAC 3 cut(s) 193, 313, 478
SaqAI TTAA 1 cut(s) 507
SatI GCNGC 5 cut(s) 90, 138, 238, 256, 319
Sau3AI GATC 1 cut(s) 295
Sau96I GGNCC 3 cut(s) 69, 91, 282
ScaI AGTACT 1 cut(s) 314
SchI GAGTC 2 cut(s) 102, 136
ScrFI CCNGG 1 cut(s) 185
SduI GDGCHC 1 cut(s) 204
SfcI CTRYAG 1 cut(s) 316
SinI GGWCC 1 cut(s) 282
SmlI CTYRAG 1 cut(s) 356
SmoI CTYRAG 1 cut(s) 356
Sse9I AATT 2 cut(s) 118, 385
SsiI CCGC 5 cut(s) 89, 96, 138, 273, 498
StyD4I CCNGG 1 cut(s) 183
TaiI ACGT 3 cut(s) 183, 198, 227
TaqI TCGA 5 cut(s) 55, 106, 122, 219, 333
TasI AATT 2 cut(s) 118, 385
TatI WGTACW 1 cut(s) 312
TauI GCSGC 2 cut(s) 92, 140
TfiI GAWTC 3 cut(s) 57, 103, 335
Tru1I TTAA 1 cut(s) 507
Tru9I TTAA 1 cut(s) 507
TseFI GTSAC 1 cut(s) 244
TseI GCWGC 3 cut(s) 237, 255, 318
Tsp45I GTSAC 1 cut(s) 244
TspGWI ACGGA 3 cut(s) 191, 256, 478
VpaK11BI GGWCC 1 cut(s) 282
XapI RAATTY 2 cut(s) 118, 385
XcmI CCANNNNNNNNNTGG 1 cut(s) 181
XmiI GTMKAC 1 cut(s) 10
ZraI GACGTC 1 cut(s) 181
ZrmI AGTACT 1 cut(s) 314
Zsp2I ATGCAT 1 cut(s) 345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.