Rroxscaffold_7G00163200

Peptidyl-tRNA hydrolase PTH2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0010251
Physical Location & Seq
Forward (+)
5551420 .. 5552672
1253 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00163200.1

Sequence Viewer

Length: 408 bp
ATGTCTCAACCGGCCTCGGAAGCCAATCCGGACACCTCGAAATCCGAGACCCCCGACGTCCTCGTTCAGTACGTGGTGCTCCGGCGAGACCTAATCGACACGTGGCCGCTGGGCAGTGTGGTAACACAGGGCTGCCATGCCTCCGTCTCCGCCATCTGGTCCCACAAAGACGACCCACACACGCTCCGGTATTGTGGCCCTGAAAATATCGACTCTATGCATAAGGTTACTCTTGAGGTGAAGGGAGAACCTCAAATATTGAACTTGTCTGAGAAGCTCAAAGCTGGTGGCATAGAGCACAAACTGTGGATTGAGCAACCTGAGAATTTCCCAACTTGTCTCGCTACGAAACCTTACCCCAAATCCATCGTATCTTCGTATTTTAAAAAGTTGAAGCTCTGTAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTH2

Protein Analysis

135

Amino Acids

15.12

Weight (kDa)

6.95

Isoelectric Point (pI)

50.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTH2 PF01981 22 - 134 1.8e-24 Peptidyl-tRNA hydrolase PTH2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010251)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10700 AT5G10700
fragaria_vesca FvH4_2g36680
malus_domestica MD08G1127800.v1.1 MD15G1107200.v1.1
prunus_persica Prupe.1G459500_v2.0.a1
pyrus_communis pycom08g10680 pycom15g09800
rosa_chinensis RchiOBHm_Chr1g0366021 RchiOBHm_Chr6g0305051
rosa_laevigata RLG00000010923
rosa_multiflora Rmu_sc0017427.1_g000001
rosa_roxburghii Rroxscaffold_7G00163200
rosa_rugosa Rorug06G0337000
rosa_samantha Rh1AG334900 Rh6AG448600 Rh6BG452300 Rh6CG462300 Rh6DG449200
rosa_wichuraiana Rw1G029710 Rw6G039080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 60
AccIII TCCGGA 1 cut(s) 28
AciI CCGC 2 cut(s) 107, 150
AcoI YGGCCR 1 cut(s) 104
AcsI RAATTY 1 cut(s) 325
AcvI CACGTG 1 cut(s) 102
AcyI GRCGYC 1 cut(s) 57
AfaI GTAC 1 cut(s) 71
AfiI CCNNNNNNNGG 1 cut(s) 156
AflIII ACRYGT 1 cut(s) 99
AgsI TTSAA 2 cut(s) 262, 394
AluBI AGCT 3 cut(s) 277, 284, 397
AluI AGCT 3 cut(s) 277, 284, 397
Alw21I GWGCWC 2 cut(s) 81, 300
Alw26I GTCTC 5 cut(s) 9, 41, 81, 151, 344
Aor13HI TCCGGA 1 cut(s) 28
AoxI GGCC 3 cut(s) 12, 104, 196
ApeKI GCWGC 1 cut(s) 132
ApoI RAATTY 1 cut(s) 325
AspS9I GGNCC 2 cut(s) 159, 197
AsuHPI GGTGA 1 cut(s) 250
AvaII GGWCC 1 cut(s) 159
BbrPI CACGTG 1 cut(s) 102
Bbv12I GWGCWC 2 cut(s) 81, 300
BbvI GCAGC 1 cut(s) 119
BccI CCATC 2 cut(s) 161, 374
BcoDI GTCTC 5 cut(s) 9, 41, 81, 151, 344
BisI GCNGC 2 cut(s) 107, 133
BlsI GCNGC 2 cut(s) 108, 134
Bme18I GGWCC 1 cut(s) 159
BmgT120I GGNCC 2 cut(s) 159, 197
BmiI GGNNCC 1 cut(s) 161
BpuEI CTTGAG 1 cut(s) 254
BsaAI YACGTR 2 cut(s) 73, 102
BsaHI GRCGYC 1 cut(s) 57
BsaI GGTCTC 2 cut(s) 41, 81
BsaJI CCNNGG 1 cut(s) 15
BsaWI WCCGGW 2 cut(s) 28, 186
BsaXI ACNNNNNCTCC 2 cut(s) 168, 198
Bsc4I CCNNNNNNNGG 1 cut(s) 156
Bse118I RCCGGY 1 cut(s) 10
BseAI TCCGGA 1 cut(s) 28
BseDI CCNNGG 1 cut(s) 15
BseLI CCNNNNNNNGG 1 cut(s) 156
BseMII CTCAG 2 cut(s) 261, 312
BseXI GCAGC 1 cut(s) 119
BseYI CCCAGC 1 cut(s) 109
BshFI GGCC 3 cut(s) 14, 106, 198
BsiHKAI GWGCWC 2 cut(s) 81, 300
BsiSI CCGG 4 cut(s) 11, 29, 82, 187
BslFI GGGAC 1 cut(s) 145
BslI CCNNNNNNNGG 1 cut(s) 156
BsmAI GTCTC 5 cut(s) 9, 41, 81, 151, 344
BsmBI CGTCTC 1 cut(s) 151
BsmFI GGGAC 1 cut(s) 145
BsnI GGCC 3 cut(s) 14, 106, 198
Bso31I GGTCTC 2 cut(s) 41, 81
Bsp1286I GDGCHC 2 cut(s) 81, 300
Bsp13I TCCGGA 1 cut(s) 28
BspACI CCGC 2 cut(s) 107, 150
BspANI GGCC 3 cut(s) 14, 106, 198
BspCNI CTCAG 2 cut(s) 262, 313
BspEI TCCGGA 1 cut(s) 28
BspLI GGNNCC 1 cut(s) 161
BspTNI GGTCTC 2 cut(s) 41, 81
BsrFI RCCGGY 1 cut(s) 10
BssAI RCCGGY 1 cut(s) 10
BssECI CCNNGG 1 cut(s) 15
BssNI GRCGYC 1 cut(s) 57
Bst4CI ACNGT 1 cut(s) 306
BstACI GRCGYC 1 cut(s) 57
BstBAI YACGTR 2 cut(s) 73, 102
BstDEI CTNAG 2 cut(s) 270, 321
BstMAI GTCTC 5 cut(s) 9, 41, 81, 151, 344
BstMWI GCNNNNNNNGC 1 cut(s) 20
BstV1I GCAGC 1 cut(s) 119
BsuRI GGCC 3 cut(s) 14, 106, 198
BtsI GCAGTG 1 cut(s) 121
BtsIMutI CAGTG 1 cut(s) 121
Cfr10I RCCGGY 1 cut(s) 10
Cfr13I GGNCC 2 cut(s) 159, 197
Csp6I GTAC 1 cut(s) 70
CspCI CAANNNNNGTGG 2 cut(s) 268, 303
CviAII CATG 1 cut(s) 137
CviJI RGCY 8 cut(s) 14, 23, 106, 132, 198, 277, 284, 397
CviKI_1 RGCY 8 cut(s) 14, 23, 106, 132, 198, 277, 284, 397
CviQI GTAC 1 cut(s) 70
DdeI CTNAG 2 cut(s) 270, 321
DraI TTTAAA 1 cut(s) 385
EaeI YGGCCR 1 cut(s) 104
EciI GGCGGA 1 cut(s) 139
Eco31I GGTCTC 2 cut(s) 41, 81
Eco47I GGWCC 1 cut(s) 159
Eco72I CACGTG 1 cut(s) 102
EcoT22I ATGCAT 1 cut(s) 222
Esp3I CGTCTC 1 cut(s) 151
FaeI CATG 1 cut(s) 140
FaiI YATR 4 cut(s) 138, 218, 222, 293
FaqI GGGAC 1 cut(s) 145
FatI CATG 1 cut(s) 136
Fnu4HI GCNGC 2 cut(s) 107, 133
Fsp4HI GCNGC 2 cut(s) 107, 133
GluI GCNGC 2 cut(s) 107, 133
GsaI CCCAGC 1 cut(s) 113
HaeIII GGCC 3 cut(s) 14, 106, 198
HapII CCGG 4 cut(s) 11, 29, 82, 187
Hin1I GRCGYC 1 cut(s) 57
Hin1II CATG 1 cut(s) 140
HinfI GANTC 1 cut(s) 212
HpaII CCGG 4 cut(s) 11, 29, 82, 187
HphI GGTGA 1 cut(s) 250
Hpy188I TCNGA 3 cut(s) 19, 46, 271
Hpy188III TCNNGA 2 cut(s) 29, 233
Hpy99I CGWCG 1 cut(s) 59
HpyAV CCTTC 1 cut(s) 235
HpyCH4III ACNGT 1 cut(s) 306
HpyCH4IV ACGT 3 cut(s) 57, 72, 101
HpyCH4V TGCA 1 cut(s) 220
HpyF10VI GCNNNNNNNGC 1 cut(s) 20
HpyF3I CTNAG 2 cut(s) 270, 321
HpySE526I ACGT 3 cut(s) 57, 72, 101
Hsp92I GRCGYC 1 cut(s) 57
Hsp92II CATG 1 cut(s) 140
Kpn2I TCCGGA 1 cut(s) 28
LmnI GCTCC 2 cut(s) 84, 189
Lsp1109I GCAGC 1 cut(s) 119
MaeII ACGT 3 cut(s) 57, 72, 101
MaeIII GTNAC 2 cut(s) 121, 226
MboII GAAGA 1 cut(s) 366
MhlI GDGCHC 2 cut(s) 81, 300
MluCI AATT 1 cut(s) 325
MlyI GAGTC 1 cut(s) 206
MnlI CCTC 6 cut(s) 25, 46, 71, 151, 229, 261
Mph1103I ATGCAT 1 cut(s) 222
MroI TCCGGA 1 cut(s) 28
MseI TTAA 1 cut(s) 384
MspA1I CMGCKG 1 cut(s) 109
MspI CCGG 4 cut(s) 11, 29, 82, 187
MwoI GCNNNNNNNGC 1 cut(s) 20
NlaIII CATG 1 cut(s) 140
NlaIV GGNNCC 1 cut(s) 161
NsiI ATGCAT 1 cut(s) 222
PcsI WCGNNNNNNNCGW 1 cut(s) 69
PkrI GCNGC 2 cut(s) 108, 134
PleI GAGTC 1 cut(s) 206
PmaCI CACGTG 1 cut(s) 102
PmlI CACGTG 1 cut(s) 102
PpsI GAGTC 1 cut(s) 206
Ppu21I YACGTR 2 cut(s) 73, 102
PspCI CACGTG 1 cut(s) 102
PspFI CCCAGC 1 cut(s) 109
PspN4I GGNNCC 1 cut(s) 161
PspPI GGNCC 2 cut(s) 159, 197
RsaI GTAC 1 cut(s) 71
RsaNI GTAC 1 cut(s) 70
SaqAI TTAA 1 cut(s) 384
SatI GCNGC 2 cut(s) 107, 133
Sau96I GGNCC 2 cut(s) 159, 197
SchI GAGTC 1 cut(s) 206
SduI GDGCHC 2 cut(s) 81, 300
SinI GGWCC 1 cut(s) 159
SmlI CTYRAG 1 cut(s) 233
SmoI CTYRAG 1 cut(s) 233
Sse9I AATT 1 cut(s) 325
SsiI CCGC 2 cut(s) 107, 150
SspI AATATT 1 cut(s) 258
TaaI ACNGT 1 cut(s) 306
TaiI ACGT 3 cut(s) 60, 75, 104
TaqI TCGA 3 cut(s) 38, 96, 210
TasI AATT 1 cut(s) 325
TauI GCSGC 1 cut(s) 109
Tru1I TTAA 1 cut(s) 384
Tru9I TTAA 1 cut(s) 384
TscAI CASTG 1 cut(s) 121
TseI GCWGC 1 cut(s) 132
TspGWI ACGGA 1 cut(s) 133
TspRI CASTG 1 cut(s) 121
VpaK11BI GGWCC 1 cut(s) 159
XapI RAATTY 1 cut(s) 325
ZraI GACGTC 1 cut(s) 58
Zsp2I ATGCAT 1 cut(s) 222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.