MD15G1107200.v1.1

peptidyl-tRNA hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0010251
Physical Location & Seq
Forward (+)
7500801 .. 7501893
1093 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1107200.v1.1.491

Sequence Viewer

Length: 561 bp
ATGGCGTGTCTACTCGCGCCACTTCGTCCCACCTCGCCCTCACTCTCAATCCCTCGAACCAGATTTGGGGCCAAACCTCCGTCCAACGCGGCCCCCGCCCCGAGTCGACTTATTCACAACTCGATGAGCCAACCCGCAGCCGAAGGCAATCCGGACGTCCCAAAGTCCGACACCACGGACGTCCTGGTTCAGTACGTCGTGCTCCGGCGAGACCTAATCGACACGTGGCCGATGGGCAGCGTGGTCACACAGGGCTGCCATGCTTCCGCGTCCGCCATTTGGTCCCCCACAAAGACGACCCCGTCACCCTCCAGTACTGTGGCCCGGAAAACATCGATTCTATGCATAAGGGTTTTGGTTACCAAATGTGGAATGAAGGTTACTCTTGAGGTGAAGGGTGAACCCCAGATATTGAATTTGTCAGAGAAGCTGAAAGCAGGTGGCATAGCGCACAAGCTGTGGATTGAGCAACCCGAAAACTTCCCAACCTGTCTCGCCACAAAGCCCTACCCCAAATCCGCGGTATCGGCGTATTTTAAAAAGTTGAAACTCTGCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTH2

Protein Analysis

187

Amino Acids

20.05

Weight (kDa)

9.75

Isoelectric Point (pI)

51.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTH2 PF01981 63 - 185 2.2e-20 Peptidyl-tRNA hydrolase PTH2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010251)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10700 AT5G10700
fragaria_vesca FvH4_2g36680
malus_domestica MD08G1127800.v1.1 MD15G1107200.v1.1
prunus_persica Prupe.1G459500_v2.0.a1
pyrus_communis pycom08g10680 pycom15g09800
rosa_chinensis RchiOBHm_Chr1g0366021 RchiOBHm_Chr6g0305051
rosa_laevigata RLG00000010923
rosa_multiflora Rmu_sc0017427.1_g000001
rosa_roxburghii Rroxscaffold_7G00163200
rosa_rugosa Rorug06G0337000
rosa_samantha Rh1AG334900 Rh6AG448600 Rh6BG452300 Rh6CG462300 Rh6DG449200
rosa_wichuraiana Rw1G029710 Rw6G039080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 428
AasI GACNNNNNNGTC 1 cut(s) 301
AatII GACGTC 2 cut(s) 159, 183
Acc36I ACCTGC 1 cut(s) 428
AccI GTMKAC 2 cut(s) 10, 106
AccII CGCG 4 cut(s) 17, 89, 269, 521
AccIII TCCGGA 1 cut(s) 151
AciI CCGC 7 cut(s) 89, 96, 135, 267, 273, 519, 521
AcoI YGGCCR 1 cut(s) 227
AcsI RAATTY 1 cut(s) 415
AcvI CACGTG 1 cut(s) 225
AcyI GRCGYC 2 cut(s) 156, 180
AfaI GTAC 2 cut(s) 194, 316
AfiI CCNNNNNNNGG 2 cut(s) 66, 279
AflIII ACRYGT 1 cut(s) 222
AgsI TTSAA 2 cut(s) 415, 547
AjnI CCWGG 1 cut(s) 183
AluBI AGCT 2 cut(s) 430, 457
AluI AGCT 2 cut(s) 430, 457
Alw21I GWGCWC 1 cut(s) 204
Alw26I GTCTC 2 cut(s) 204, 497
Ama87I CYCGRG 1 cut(s) 100
Aor13HI TCCGGA 1 cut(s) 151
AoxI GGCC 4 cut(s) 69, 90, 227, 321
ApeKI GCWGC 3 cut(s) 137, 237, 255
ApoI RAATTY 1 cut(s) 415
AspLEI GCGC 2 cut(s) 19, 451
AspS9I GGNCC 4 cut(s) 69, 91, 282, 322
AsuC2I CCSGG 1 cut(s) 325
AsuHPI GGTGA 3 cut(s) 297, 403, 410
AvaI CYCGRG 1 cut(s) 100
AvaII GGWCC 1 cut(s) 282
BbrPI CACGTG 1 cut(s) 225
Bbv12I GWGCWC 1 cut(s) 204
BbvI GCAGC 3 cut(s) 149, 242, 249
BccI CCATC 1 cut(s) 226
BciT130I CCWGG 1 cut(s) 185
BcnI CCSGG 1 cut(s) 325
BcoDI GTCTC 2 cut(s) 204, 497
BfuAI ACCTGC 1 cut(s) 428
BisI GCNGC 4 cut(s) 90, 138, 238, 256
BlsI GCNGC 4 cut(s) 91, 139, 239, 257
BmcAI AGTACT 1 cut(s) 316
Bme1390I CCNGG 2 cut(s) 185, 325
Bme18I GGWCC 1 cut(s) 282
BmeT110I CYCGRG 1 cut(s) 100
BmgT120I GGNCC 4 cut(s) 69, 91, 282, 322
BmiI GGNNCC 3 cut(s) 70, 93, 284
BmrFI CCNGG 2 cut(s) 185, 325
BpmI CTGGAG 1 cut(s) 295
BpuEI CTTGAG 1 cut(s) 407
BpuMI CCSGG 1 cut(s) 325
Bsa29I ATCGAT 1 cut(s) 335
BsaAI YACGTR 1 cut(s) 225
BsaHI GRCGYC 2 cut(s) 156, 180
BsaI GGTCTC 1 cut(s) 204
BsaJI CCNNGG 2 cut(s) 174, 519
BsaWI WCCGGW 1 cut(s) 151
Bsc4I CCNNNNNNNGG 2 cut(s) 66, 279
Bse1I ACTGG 1 cut(s) 312
BseAI TCCGGA 1 cut(s) 151
BseBI CCWGG 1 cut(s) 185
BseCI ATCGAT 1 cut(s) 335
BseDI CCNNGG 2 cut(s) 174, 519
BseLI CCNNNNNNNGG 2 cut(s) 66, 279
BseNI ACTGG 1 cut(s) 312
BseXI GCAGC 3 cut(s) 149, 242, 249
Bsh1236I CGCG 4 cut(s) 17, 89, 269, 521
BshFI GGCC 4 cut(s) 71, 92, 229, 323
BshVI ATCGAT 1 cut(s) 335
BsiHKAI GWGCWC 1 cut(s) 204
BsiHKCI CYCGRG 1 cut(s) 100
BsiSI CCGG 3 cut(s) 152, 205, 325
BslFI GGGAC 3 cut(s) 12, 143, 268
BslI CCNNNNNNNGG 2 cut(s) 66, 279
BsmAI GTCTC 2 cut(s) 204, 497
BsmFI GGGAC 3 cut(s) 12, 143, 268
BsnI GGCC 4 cut(s) 71, 92, 229, 323
Bso31I GGTCTC 1 cut(s) 204
BsoBI CYCGRG 1 cut(s) 100
Bsp1286I GDGCHC 1 cut(s) 204
Bsp13I TCCGGA 1 cut(s) 151
BspACI CCGC 7 cut(s) 89, 96, 135, 267, 273, 519, 521
BspANI GGCC 4 cut(s) 71, 92, 229, 323
BspDI ATCGAT 1 cut(s) 335
BspEI TCCGGA 1 cut(s) 151
BspFNI CGCG 4 cut(s) 17, 89, 269, 521
BspLI GGNNCC 3 cut(s) 70, 93, 284
BspMI ACCTGC 1 cut(s) 428
BspTNI GGTCTC 1 cut(s) 204
BsrI ACTGG 1 cut(s) 312
BssECI CCNNGG 2 cut(s) 174, 519
BssNI GRCGYC 2 cut(s) 156, 180
Bst2UI CCWGG 1 cut(s) 185
Bst4CI ACNGT 1 cut(s) 319
BstACI GRCGYC 2 cut(s) 156, 180
BstBAI YACGTR 1 cut(s) 225
BstDSI CCRYGG 2 cut(s) 174, 519
BstEII GGTNACC 1 cut(s) 358
BstFNI CGCG 4 cut(s) 17, 89, 269, 521
BstHHI GCGC 2 cut(s) 19, 451
BstMAI GTCTC 2 cut(s) 204, 497
BstMWI GCNNNNNNNGC 2 cut(s) 95, 527
BstNI CCWGG 1 cut(s) 185
BstPI GGTNACC 1 cut(s) 358
BstSCI CCNGG 2 cut(s) 183, 323
BstUI CGCG 4 cut(s) 17, 89, 269, 521
BstV1I GCAGC 3 cut(s) 149, 242, 249
BstXI CCANNNNNNTGG 1 cut(s) 319
Bsu15I ATCGAT 1 cut(s) 335
BsuRI GGCC 4 cut(s) 71, 92, 229, 323
BsuTUI ATCGAT 1 cut(s) 335
BtgI CCRYGG 2 cut(s) 174, 519
BveI ACCTGC 1 cut(s) 428
CfoI GCGC 2 cut(s) 19, 451
Cfr13I GGNCC 4 cut(s) 69, 91, 282, 322
Cfr42I CCGCGG 1 cut(s) 522
ClaI ATCGAT 1 cut(s) 335
CseI GACGC 1 cut(s) 258
Csp6I GTAC 2 cut(s) 193, 315
CviAII CATG 1 cut(s) 260
CviQI GTAC 2 cut(s) 193, 315
DraI TTTAAA 1 cut(s) 538
DrdI GACNNNNNNGTC 1 cut(s) 301
DseDI GACNNNNNNGTC 1 cut(s) 301
EaeI YGGCCR 1 cut(s) 227
EciI GGCGGA 1 cut(s) 262
Eco31I GGTCTC 1 cut(s) 204
Eco47I GGWCC 1 cut(s) 282
Eco72I CACGTG 1 cut(s) 225
Eco88I CYCGRG 1 cut(s) 100
Eco91I GGTNACC 1 cut(s) 358
EcoO65I GGTNACC 1 cut(s) 358
EcoRII CCWGG 1 cut(s) 183
EcoT22I ATGCAT 1 cut(s) 347
FaeI CATG 1 cut(s) 263
FaiI YATR 4 cut(s) 261, 343, 347, 446
FaqI GGGAC 3 cut(s) 12, 143, 268
FatI CATG 1 cut(s) 259
FauI CCCGC 2 cut(s) 103, 142
FblI GTMKAC 2 cut(s) 10, 106
Fnu4HI GCNGC 4 cut(s) 90, 138, 238, 256
Fsp4HI GCNGC 4 cut(s) 90, 138, 238, 256
GlaI GCGC 2 cut(s) 18, 450
GluI GCNGC 4 cut(s) 90, 138, 238, 256
GsuI CTGGAG 1 cut(s) 295
HaeIII GGCC 4 cut(s) 71, 92, 229, 323
HapII CCGG 3 cut(s) 152, 205, 325
HgaI GACGC 1 cut(s) 258
HhaI GCGC 2 cut(s) 19, 451
Hin1I GRCGYC 2 cut(s) 156, 180
Hin1II CATG 1 cut(s) 263
Hin6I GCGC 2 cut(s) 17, 449
HinP1I GCGC 2 cut(s) 17, 449
HincII GTYRAC 1 cut(s) 107
HindII GTYRAC 1 cut(s) 107
HinfI GANTC 2 cut(s) 103, 337
HpaII CCGG 3 cut(s) 152, 205, 325
HphI GGTGA 3 cut(s) 297, 403, 410
Hpy166II GTNNAC 3 cut(s) 11, 107, 401
Hpy188I TCNGA 2 cut(s) 169, 424
Hpy188III TCNNGA 2 cut(s) 152, 386
Hpy8I GTNNAC 3 cut(s) 11, 107, 401
Hpy99I CGWCG 1 cut(s) 200
HpyAV CCTTC 3 cut(s) 137, 370, 388
HpyCH4III ACNGT 1 cut(s) 319
HpyCH4IV ACGT 4 cut(s) 156, 180, 195, 224
HpyCH4V TGCA 2 cut(s) 345, 555
HpyF10VI GCNNNNNNNGC 2 cut(s) 95, 527
HpySE526I ACGT 4 cut(s) 156, 180, 195, 224
Hsp92I GRCGYC 2 cut(s) 156, 180
Hsp92II CATG 1 cut(s) 263
HspAI GCGC 2 cut(s) 17, 449
Kpn2I TCCGGA 1 cut(s) 151
KspI CCGCGG 1 cut(s) 522
LmnI GCTCC 1 cut(s) 207
Lsp1109I GCAGC 3 cut(s) 149, 242, 249
MaeII ACGT 4 cut(s) 156, 180, 195, 224
MaeIII GTNAC 4 cut(s) 244, 303, 358, 379
MhlI GDGCHC 1 cut(s) 204
MluCI AATT 1 cut(s) 415
MlyI GAGTC 1 cut(s) 112
MmeI TCCRAC 2 cut(s) 108, 192
MnlI CCTC 6 cut(s) 43, 49, 63, 87, 319, 382
Mph1103I ATGCAT 1 cut(s) 347
MroI TCCGGA 1 cut(s) 151
MseI TTAA 1 cut(s) 537
MspA1I CMGCKG 1 cut(s) 521
MspI CCGG 3 cut(s) 152, 205, 325
MspR9I CCNGG 2 cut(s) 185, 325
MvaI CCWGG 1 cut(s) 185
MvnI CGCG 4 cut(s) 17, 89, 269, 521
MwoI GCNNNNNNNGC 2 cut(s) 95, 527
NciI CCSGG 1 cut(s) 325
NlaIII CATG 1 cut(s) 263
NlaIV GGNNCC 3 cut(s) 70, 93, 284
NmuCI GTSAC 2 cut(s) 244, 303
NsiI ATGCAT 1 cut(s) 347
PaqCI CACCTGC 1 cut(s) 428
PfeI GAWTC 1 cut(s) 337
PflFI GACNNNGTC 1 cut(s) 301
PkrI GCNGC 4 cut(s) 91, 139, 239, 257
PleI GAGTC 1 cut(s) 111
PmaCI CACGTG 1 cut(s) 225
PmlI CACGTG 1 cut(s) 225
PpsI GAGTC 1 cut(s) 111
Ppu21I YACGTR 1 cut(s) 225
Psp6I CCWGG 1 cut(s) 183
PspCI CACGTG 1 cut(s) 225
PspEI GGTNACC 1 cut(s) 358
PspGI CCWGG 1 cut(s) 183
PspN4I GGNNCC 3 cut(s) 70, 93, 284
PspPI GGNCC 4 cut(s) 69, 91, 282, 322
PsyI GACNNNGTC 1 cut(s) 301
RsaI GTAC 2 cut(s) 194, 316
RsaNI GTAC 2 cut(s) 193, 315
SacII CCGCGG 1 cut(s) 522
SalI GTCGAC 1 cut(s) 105
SaqAI TTAA 1 cut(s) 537
SatI GCNGC 4 cut(s) 90, 138, 238, 256
Sau96I GGNCC 4 cut(s) 69, 91, 282, 322
ScaI AGTACT 1 cut(s) 316
SchI GAGTC 1 cut(s) 112
ScrFI CCNGG 2 cut(s) 185, 325
SduI GDGCHC 1 cut(s) 204
Sfr303I CCGCGG 1 cut(s) 522
SgrBI CCGCGG 1 cut(s) 522
SinI GGWCC 1 cut(s) 282
SmlI CTYRAG 1 cut(s) 386
SmoI CTYRAG 1 cut(s) 386
Sse9I AATT 1 cut(s) 415
SsiI CCGC 7 cut(s) 89, 96, 135, 267, 273, 519, 521
StyD4I CCNGG 2 cut(s) 183, 323
TaaI ACNGT 1 cut(s) 319
TaiI ACGT 4 cut(s) 159, 183, 198, 227
TaqI TCGA 5 cut(s) 55, 106, 122, 219, 335
TasI AATT 1 cut(s) 415
TatI WGTACW 1 cut(s) 314
TauI GCSGC 1 cut(s) 92
TfiI GAWTC 1 cut(s) 337
Tru1I TTAA 1 cut(s) 537
Tru9I TTAA 1 cut(s) 537
TseFI GTSAC 2 cut(s) 244, 303
TseI GCWGC 3 cut(s) 137, 237, 255
Tsp45I GTSAC 2 cut(s) 244, 303
TspDTI ATGAA 1 cut(s) 389
TspGWI ACGGA 2 cut(s) 69, 191
Tth111I GACNNNGTC 1 cut(s) 301
VpaK11BI GGWCC 1 cut(s) 282
XapI RAATTY 1 cut(s) 415
XcmI CCANNNNNNNNNTGG 1 cut(s) 181
XmiI GTMKAC 2 cut(s) 10, 106
ZraI GACGTC 2 cut(s) 157, 181
ZrmI AGTACT 1 cut(s) 316
Zsp2I ATGCAT 1 cut(s) 347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.