RchiOBHm_Chr6g0305051

peptidyl-tRNA hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0010251
Physical Location & Seq
Reverse (-)
64133022 .. 64134314
1293 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27413

Sequence Viewer

Length: 531 bp
ATGGCCTGTATTGTCTGGCCACTTCGTTTCACTCCCCCCCCCCCCCCCCCCCCCCTTTCTCATCTCTCAAGCACCCGAGTTGGATTCGGGCCAAAACCCCCGAGTCGACTCGGATTCAACTCAATGTCTCAACCGGCCTCGGAAGCCAATCCGGACACCTCGAAACCCGAGACCCCCGACGTCCTCGTTCAGTACGTGGTGCTCCGGCGAGACCTAATCGACACGTGGCCGCTGGGCAGTGTGGTAACACAGGGCTGCCATGCTTCCGTCTCCGCCATCCGGTCCCACAAAGACGACCCACGCACGCTCCAGTATTGTAGCCCTGAAAATATCGATTCTATGCATAAGGTTACTCTTGAGGTGAAGGGAGAACCTCAAATATTGAACTTGTCTGAGAAGCTCAAAGCTGGTGGCATAGAGCACAAGCTGTGGATTGAGCAACCTGAGAATTTCCCAACTTGTCTCGCTACGAAACCTTACCCCAAATCCATCGTATCTTCGTATTTTAAAAAGTTGAAGCTCTGTAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTH2

Protein Analysis

176

Amino Acids

19.54

Weight (kDa)

8.98

Isoelectric Point (pI)

60.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTH2 PF01981 63 - 175 1.8e-24 Peptidyl-tRNA hydrolase PTH2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010251)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10700 AT5G10700
fragaria_vesca FvH4_2g36680
malus_domestica MD08G1127800.v1.1 MD15G1107200.v1.1
prunus_persica Prupe.1G459500_v2.0.a1
pyrus_communis pycom08g10680 pycom15g09800
rosa_chinensis RchiOBHm_Chr1g0366021 RchiOBHm_Chr6g0305051
rosa_laevigata RLG00000010923
rosa_multiflora Rmu_sc0017427.1_g000001
rosa_roxburghii Rroxscaffold_7G00163200
rosa_rugosa Rorug06G0337000
rosa_samantha Rh1AG334900 Rh6AG448600 Rh6BG452300 Rh6CG462300 Rh6DG449200
rosa_wichuraiana Rw1G029710 Rw6G039080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 183
AccI GTMKAC 1 cut(s) 106
AccIII TCCGGA 1 cut(s) 151
AciI CCGC 2 cut(s) 230, 273
AcoI YGGCCR 2 cut(s) 17, 227
AcsI RAATTY 1 cut(s) 448
AcvI CACGTG 1 cut(s) 225
AcyI GRCGYC 1 cut(s) 180
AfaI GTAC 1 cut(s) 194
AfiI CCNNNNNNNGG 1 cut(s) 279
AflIII ACRYGT 1 cut(s) 222
AgsI TTSAA 3 cut(s) 118, 385, 517
AluBI AGCT 4 cut(s) 400, 407, 427, 520
AluI AGCT 4 cut(s) 400, 407, 427, 520
Alw21I GWGCWC 2 cut(s) 204, 423
Alw26I GTCTC 5 cut(s) 132, 164, 204, 274, 467
Ama87I CYCGRG 3 cut(s) 75, 100, 167
Aor13HI TCCGGA 1 cut(s) 151
AoxI GGCC 5 cut(s) 3, 17, 89, 135, 227
ApeKI GCWGC 1 cut(s) 255
ApoI RAATTY 1 cut(s) 448
AspS9I GGNCC 2 cut(s) 89, 282
AsuHPI GGTGA 1 cut(s) 373
AvaI CYCGRG 3 cut(s) 75, 100, 167
AvaII GGWCC 1 cut(s) 282
BalI TGGCCA 1 cut(s) 19
BbrPI CACGTG 1 cut(s) 225
Bbv12I GWGCWC 2 cut(s) 204, 423
BbvI GCAGC 1 cut(s) 242
BccI CCATC 2 cut(s) 284, 497
BcoDI GTCTC 5 cut(s) 132, 164, 204, 274, 467
BisI GCNGC 2 cut(s) 230, 256
BlsI GCNGC 2 cut(s) 231, 257
Bme18I GGWCC 1 cut(s) 282
BmeT110I CYCGRG 3 cut(s) 75, 100, 167
BmgT120I GGNCC 2 cut(s) 89, 282
BmiI GGNNCC 1 cut(s) 284
BpmI CTGGAG 1 cut(s) 293
BpuEI CTTGAG 2 cut(s) 52, 377
Bsa29I ATCGAT 1 cut(s) 333
BsaAI YACGTR 2 cut(s) 196, 225
BsaHI GRCGYC 1 cut(s) 180
BsaI GGTCTC 2 cut(s) 164, 204
BsaJI CCNNGG 1 cut(s) 138
BsaWI WCCGGW 2 cut(s) 151, 279
BsaXI ACNNNNNCTCC 2 cut(s) 291, 321
Bsc4I CCNNNNNNNGG 1 cut(s) 279
Bse118I RCCGGY 1 cut(s) 133
Bse1I ACTGG 1 cut(s) 310
BseAI TCCGGA 1 cut(s) 151
BseCI ATCGAT 1 cut(s) 333
BseDI CCNNGG 1 cut(s) 138
BseGI GGATG 1 cut(s) 276
BseLI CCNNNNNNNGG 1 cut(s) 279
BseMII CTCAG 2 cut(s) 384, 435
BseNI ACTGG 1 cut(s) 310
BseXI GCAGC 1 cut(s) 242
BseYI CCCAGC 1 cut(s) 232
BshFI GGCC 5 cut(s) 5, 19, 91, 137, 229
BshVI ATCGAT 1 cut(s) 333
BsiHKAI GWGCWC 2 cut(s) 204, 423
BsiHKCI CYCGRG 3 cut(s) 75, 100, 167
BsiSI CCGG 4 cut(s) 134, 152, 205, 280
BslFI GGGAC 1 cut(s) 268
BslI CCNNNNNNNGG 1 cut(s) 279
BsmAI GTCTC 5 cut(s) 132, 164, 204, 274, 467
BsmBI CGTCTC 1 cut(s) 274
BsmFI GGGAC 1 cut(s) 268
BsnI GGCC 5 cut(s) 5, 19, 91, 137, 229
Bso31I GGTCTC 2 cut(s) 164, 204
BsoBI CYCGRG 3 cut(s) 75, 100, 167
Bsp1286I GDGCHC 2 cut(s) 204, 423
Bsp13I TCCGGA 1 cut(s) 151
BspACI CCGC 2 cut(s) 230, 273
BspANI GGCC 5 cut(s) 5, 19, 91, 137, 229
BspCNI CTCAG 2 cut(s) 385, 436
BspDI ATCGAT 1 cut(s) 333
BspEI TCCGGA 1 cut(s) 151
BspLI GGNNCC 1 cut(s) 284
BspTNI GGTCTC 2 cut(s) 164, 204
BsrFI RCCGGY 1 cut(s) 133
BsrI ACTGG 1 cut(s) 310
BssAI RCCGGY 1 cut(s) 133
BssECI CCNNGG 1 cut(s) 138
BssNI GRCGYC 1 cut(s) 180
BstACI GRCGYC 1 cut(s) 180
BstBAI YACGTR 2 cut(s) 196, 225
BstC8I GCNNGC 1 cut(s) 305
BstDEI CTNAG 2 cut(s) 393, 444
BstF5I GGATG 1 cut(s) 276
BstMAI GTCTC 5 cut(s) 132, 164, 204, 274, 467
BstMWI GCNNNNNNNGC 1 cut(s) 143
BstV1I GCAGC 1 cut(s) 242
Bsu15I ATCGAT 1 cut(s) 333
BsuRI GGCC 5 cut(s) 5, 19, 91, 137, 229
BsuTUI ATCGAT 1 cut(s) 333
BtsCI GGATG 1 cut(s) 276
BtsI GCAGTG 1 cut(s) 244
BtsIMutI CAGTG 1 cut(s) 244
Cac8I GCNNGC 1 cut(s) 305
Cfr10I RCCGGY 1 cut(s) 133
Cfr13I GGNCC 2 cut(s) 89, 282
ClaI ATCGAT 1 cut(s) 333
Csp6I GTAC 1 cut(s) 193
CspCI CAANNNNNGTGG 2 cut(s) 391, 426
CviAII CATG 1 cut(s) 260
CviQI GTAC 1 cut(s) 193
DdeI CTNAG 2 cut(s) 393, 444
DraI TTTAAA 1 cut(s) 508
EaeI YGGCCR 2 cut(s) 17, 227
EciI GGCGGA 1 cut(s) 262
Eco31I GGTCTC 2 cut(s) 164, 204
Eco47I GGWCC 1 cut(s) 282
Eco72I CACGTG 1 cut(s) 225
Eco88I CYCGRG 3 cut(s) 75, 100, 167
EcoT22I ATGCAT 1 cut(s) 345
Esp3I CGTCTC 1 cut(s) 274
FaeI CATG 1 cut(s) 263
FaiI YATR 4 cut(s) 261, 341, 345, 416
FaqI GGGAC 1 cut(s) 268
FatI CATG 1 cut(s) 259
FblI GTMKAC 1 cut(s) 106
Fnu4HI GCNGC 2 cut(s) 230, 256
FokI GGATG 1 cut(s) 263
Fsp4HI GCNGC 2 cut(s) 230, 256
GluI GCNGC 2 cut(s) 230, 256
GsaI CCCAGC 1 cut(s) 236
GsuI CTGGAG 1 cut(s) 293
HaeIII GGCC 5 cut(s) 5, 19, 91, 137, 229
HapII CCGG 4 cut(s) 134, 152, 205, 280
Hin1I GRCGYC 1 cut(s) 180
Hin1II CATG 1 cut(s) 263
HincII GTYRAC 1 cut(s) 107
HindII GTYRAC 1 cut(s) 107
HinfI GANTC 5 cut(s) 84, 103, 108, 114, 335
HpaII CCGG 4 cut(s) 134, 152, 205, 280
HphI GGTGA 1 cut(s) 373
Hpy166II GTNNAC 1 cut(s) 107
Hpy188I TCNGA 3 cut(s) 113, 142, 394
Hpy188III TCNNGA 2 cut(s) 152, 356
Hpy8I GTNNAC 1 cut(s) 107
Hpy99I CGWCG 1 cut(s) 182
HpyAV CCTTC 1 cut(s) 358
HpyCH4IV ACGT 3 cut(s) 180, 195, 224
HpyCH4V TGCA 1 cut(s) 343
HpyF10VI GCNNNNNNNGC 1 cut(s) 143
HpyF3I CTNAG 2 cut(s) 393, 444
HpySE526I ACGT 3 cut(s) 180, 195, 224
Hsp92I GRCGYC 1 cut(s) 180
Hsp92II CATG 1 cut(s) 263
Kpn2I TCCGGA 1 cut(s) 151
LmnI GCTCC 2 cut(s) 207, 312
Lsp1109I GCAGC 1 cut(s) 242
MaeII ACGT 3 cut(s) 180, 195, 224
MaeIII GTNAC 2 cut(s) 244, 349
MboII GAAGA 1 cut(s) 489
MhlI GDGCHC 2 cut(s) 204, 423
MlsI TGGCCA 1 cut(s) 19
MluCI AATT 1 cut(s) 448
MluNI TGGCCA 1 cut(s) 19
MlyI GAGTC 2 cut(s) 102, 112
MmeI TCCRAC 1 cut(s) 61
MnlI CCTC 5 cut(s) 148, 169, 194, 352, 384
Mox20I TGGCCA 1 cut(s) 19
Mph1103I ATGCAT 1 cut(s) 345
MroI TCCGGA 1 cut(s) 151
MscI TGGCCA 1 cut(s) 19
MseI TTAA 1 cut(s) 507
Msp20I TGGCCA 1 cut(s) 19
MspA1I CMGCKG 1 cut(s) 232
MspI CCGG 4 cut(s) 134, 152, 205, 280
MwoI GCNNNNNNNGC 1 cut(s) 143
NlaIII CATG 1 cut(s) 263
NlaIV GGNNCC 1 cut(s) 284
NsiI ATGCAT 1 cut(s) 345
PcsI WCGNNNNNNNCGW 1 cut(s) 192
PfeI GAWTC 3 cut(s) 84, 114, 335
PkrI GCNGC 2 cut(s) 231, 257
PleI GAGTC 2 cut(s) 102, 111
PmaCI CACGTG 1 cut(s) 225
PmlI CACGTG 1 cut(s) 225
PpsI GAGTC 2 cut(s) 102, 111
Ppu21I YACGTR 2 cut(s) 196, 225
PspCI CACGTG 1 cut(s) 225
PspFI CCCAGC 1 cut(s) 232
PspN4I GGNNCC 1 cut(s) 284
PspPI GGNCC 2 cut(s) 89, 282
RsaI GTAC 1 cut(s) 194
RsaNI GTAC 1 cut(s) 193
SalI GTCGAC 1 cut(s) 105
SaqAI TTAA 1 cut(s) 507
SatI GCNGC 2 cut(s) 230, 256
Sau96I GGNCC 2 cut(s) 89, 282
SchI GAGTC 2 cut(s) 102, 112
SduI GDGCHC 2 cut(s) 204, 423
SinI GGWCC 1 cut(s) 282
SmlI CTYRAG 2 cut(s) 67, 356
SmoI CTYRAG 2 cut(s) 67, 356
Sse9I AATT 1 cut(s) 448
SsiI CCGC 2 cut(s) 230, 273
SspI AATATT 1 cut(s) 381
TaiI ACGT 3 cut(s) 183, 198, 227
TaqI TCGA 4 cut(s) 106, 161, 219, 333
TasI AATT 1 cut(s) 448
TauI GCSGC 1 cut(s) 232
TfiI GAWTC 3 cut(s) 84, 114, 335
Tru1I TTAA 1 cut(s) 507
Tru9I TTAA 1 cut(s) 507
TscAI CASTG 1 cut(s) 244
TseI GCWGC 1 cut(s) 255
TspGWI ACGGA 1 cut(s) 256
TspRI CASTG 1 cut(s) 244
VpaK11BI GGWCC 1 cut(s) 282
XapI RAATTY 1 cut(s) 448
XmiI GTMKAC 1 cut(s) 106
ZraI GACGTC 1 cut(s) 181
Zsp2I ATGCAT 1 cut(s) 345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.