pycom15g09800

peptidyl-tRNA hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0010251
Physical Location & Seq
Forward (+)
6386742 .. 6387629
888 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g09800.1

Sequence Viewer

Length: 534 bp
ATGGCGTGTCTACTCGCGCCACTTCGTCCCACCTCACCCTCACTCTCAAATTCAATCCCTCGAATCAGATTTGGGGCCAAACCTCCGTCCAACGCGGCCTCCGCCCAGAGTCGACTCATCCGGAACTCGATGAGTCAACCCGCCGCCGAATCCAATCCAGACGTCCCAAAGTCCGACATCACGGACGTCCTGGTTCAGTACGTCGTGCTCCGGCGAGACCTAATCGACACGTGGCCGATGGGCAGCGTGGTCACACAGGGCTGCCATGCCTCCGTGTCCGCCATTTGGTCCCACAAAGACTCCATCACGCTCCAATACTGTGGCCCGGAAAACATCGATTCTATGCATAAGGTTACTCTTGAGGTGAAGGGAGAACCCCAGATATTGAATTTGTCAGAGAAGCTCGAAGCAGGTGGCATAGCGCACAAGCTGTGGATTGAGCAACCCGAAAACTTCCCAACCTGCCTCGCCACAAAGCCCTACCCCAAATCCGCGGTATCGGCGTATTTTAAAAAGTTGAAACTCTGTAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTH2

Protein Analysis

178

Amino Acids

19.38

Weight (kDa)

8.99

Isoelectric Point (pI)

57.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTH2 PF01981 65 - 176 9.9e-24 Peptidyl-tRNA hydrolase PTH2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010251)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10700 AT5G10700
fragaria_vesca FvH4_2g36680
malus_domestica MD08G1127800.v1.1 MD15G1107200.v1.1
prunus_persica Prupe.1G459500_v2.0.a1
pyrus_communis pycom08g10680 pycom15g09800
rosa_chinensis RchiOBHm_Chr1g0366021 RchiOBHm_Chr6g0305051
rosa_laevigata RLG00000010923
rosa_multiflora Rmu_sc0017427.1_g000001
rosa_roxburghii Rroxscaffold_7G00163200
rosa_rugosa Rorug06G0337000
rosa_samantha Rh1AG334900 Rh6AG448600 Rh6BG452300 Rh6CG462300 Rh6DG449200
rosa_wichuraiana Rw1G029710 Rw6G039080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 401
AatII GACGTC 2 cut(s) 165, 189
Acc36I ACCTGC 2 cut(s) 401, 470
AccI GTMKAC 2 cut(s) 10, 112
AccII CGCG 3 cut(s) 17, 95, 494
AccIII TCCGGA 1 cut(s) 120
AciI CCGC 7 cut(s) 95, 102, 141, 144, 279, 492, 494
AcoI YGGCCR 1 cut(s) 233
AcsI RAATTY 2 cut(s) 49, 388
AcvI CACGTG 1 cut(s) 231
AcyI GRCGYC 2 cut(s) 162, 186
AfaI GTAC 1 cut(s) 200
AfiI CCNNNNNNNGG 1 cut(s) 285
AflIII ACRYGT 1 cut(s) 228
AgsI TTSAA 3 cut(s) 54, 388, 520
AjnI CCWGG 1 cut(s) 189
AluBI AGCT 2 cut(s) 403, 430
AluI AGCT 2 cut(s) 403, 430
Alw21I GWGCWC 1 cut(s) 210
Alw26I GTCTC 1 cut(s) 210
Aor13HI TCCGGA 1 cut(s) 120
AoxI GGCC 4 cut(s) 75, 96, 233, 322
ApeKI GCWGC 2 cut(s) 243, 261
ApoI RAATTY 2 cut(s) 49, 388
AspLEI GCGC 2 cut(s) 19, 424
AspS9I GGNCC 3 cut(s) 75, 288, 323
AsuC2I CCSGG 1 cut(s) 326
AsuHPI GGTGA 2 cut(s) 27, 376
AvaII GGWCC 1 cut(s) 288
BbrPI CACGTG 1 cut(s) 231
Bbv12I GWGCWC 1 cut(s) 210
BbvI GCAGC 2 cut(s) 248, 255
BccI CCATC 2 cut(s) 232, 311
BciT130I CCWGG 1 cut(s) 191
BcnI CCSGG 1 cut(s) 326
BcoDI GTCTC 1 cut(s) 210
BfuAI ACCTGC 2 cut(s) 401, 470
BisI GCNGC 4 cut(s) 96, 144, 244, 262
BlsI GCNGC 4 cut(s) 97, 145, 245, 263
Bme1390I CCNGG 2 cut(s) 191, 326
Bme18I GGWCC 1 cut(s) 288
BmgT120I GGNCC 3 cut(s) 75, 288, 323
BmiI GGNNCC 2 cut(s) 76, 290
BmrFI CCNGG 2 cut(s) 191, 326
BpuEI CTTGAG 1 cut(s) 380
BpuMI CCSGG 1 cut(s) 326
Bsa29I ATCGAT 1 cut(s) 336
BsaAI YACGTR 1 cut(s) 231
BsaHI GRCGYC 2 cut(s) 162, 186
BsaI GGTCTC 1 cut(s) 210
BsaJI CCNNGG 1 cut(s) 492
BsaWI WCCGGW 1 cut(s) 120
BsaXI ACNNNNNCTCC 4 cut(s) 83, 113, 284, 314
Bsc4I CCNNNNNNNGG 1 cut(s) 285
BseAI TCCGGA 1 cut(s) 120
BseBI CCWGG 1 cut(s) 191
BseCI ATCGAT 1 cut(s) 336
BseDI CCNNGG 1 cut(s) 492
BseGI GGATG 1 cut(s) 117
BseLI CCNNNNNNNGG 1 cut(s) 285
BseXI GCAGC 2 cut(s) 248, 255
Bsh1236I CGCG 3 cut(s) 17, 95, 494
BshFI GGCC 4 cut(s) 77, 98, 235, 324
BshVI ATCGAT 1 cut(s) 336
BsiHKAI GWGCWC 1 cut(s) 210
BsiSI CCGG 3 cut(s) 121, 211, 326
BslFI GGGAC 3 cut(s) 12, 149, 274
BslI CCNNNNNNNGG 1 cut(s) 285
BsmAI GTCTC 1 cut(s) 210
BsmFI GGGAC 3 cut(s) 12, 149, 274
BsnI GGCC 4 cut(s) 77, 98, 235, 324
Bso31I GGTCTC 1 cut(s) 210
Bsp1286I GDGCHC 1 cut(s) 210
Bsp13I TCCGGA 1 cut(s) 120
BspACI CCGC 7 cut(s) 95, 102, 141, 144, 279, 492, 494
BspANI GGCC 4 cut(s) 77, 98, 235, 324
BspDI ATCGAT 1 cut(s) 336
BspEI TCCGGA 1 cut(s) 120
BspFNI CGCG 3 cut(s) 17, 95, 494
BspLI GGNNCC 2 cut(s) 76, 290
BspMI ACCTGC 2 cut(s) 401, 470
BspTNI GGTCTC 1 cut(s) 210
BssECI CCNNGG 1 cut(s) 492
BssNI GRCGYC 2 cut(s) 162, 186
Bst2UI CCWGG 1 cut(s) 191
Bst4CI ACNGT 1 cut(s) 320
BstACI GRCGYC 2 cut(s) 162, 186
BstBAI YACGTR 1 cut(s) 231
BstDSI CCRYGG 1 cut(s) 492
BstF5I GGATG 1 cut(s) 117
BstFNI CGCG 3 cut(s) 17, 95, 494
BstHHI GCGC 2 cut(s) 19, 424
BstMAI GTCTC 1 cut(s) 210
BstMWI GCNNNNNNNGC 2 cut(s) 101, 500
BstNI CCWGG 1 cut(s) 191
BstSCI CCNGG 2 cut(s) 189, 324
BstUI CGCG 3 cut(s) 17, 95, 494
BstV1I GCAGC 2 cut(s) 248, 255
BstXI CCANNNNNNTGG 1 cut(s) 320
Bsu15I ATCGAT 1 cut(s) 336
BsuRI GGCC 4 cut(s) 77, 98, 235, 324
BsuTUI ATCGAT 1 cut(s) 336
BtgI CCRYGG 1 cut(s) 492
BtsCI GGATG 1 cut(s) 117
BveI ACCTGC 2 cut(s) 401, 470
CfoI GCGC 2 cut(s) 19, 424
Cfr13I GGNCC 3 cut(s) 75, 288, 323
Cfr42I CCGCGG 1 cut(s) 495
ClaI ATCGAT 1 cut(s) 336
Csp6I GTAC 1 cut(s) 199
CviAII CATG 1 cut(s) 266
CviJI RGCY 8 cut(s) 77, 98, 235, 261, 324, 403, 430, 478
CviKI_1 RGCY 8 cut(s) 77, 98, 235, 261, 324, 403, 430, 478
CviQI GTAC 1 cut(s) 199
DraI TTTAAA 1 cut(s) 511
EaeI YGGCCR 1 cut(s) 233
EciI GGCGGA 2 cut(s) 91, 268
Eco31I GGTCTC 1 cut(s) 210
Eco47I GGWCC 1 cut(s) 288
Eco72I CACGTG 1 cut(s) 231
EcoRII CCWGG 1 cut(s) 189
EcoT22I ATGCAT 1 cut(s) 348
FaeI CATG 1 cut(s) 269
FaiI YATR 4 cut(s) 267, 344, 348, 419
FaqI GGGAC 3 cut(s) 12, 149, 274
FatI CATG 1 cut(s) 265
FauI CCCGC 1 cut(s) 148
FblI GTMKAC 2 cut(s) 10, 112
Fnu4HI GCNGC 4 cut(s) 96, 144, 244, 262
FokI GGATG 1 cut(s) 104
Fsp4HI GCNGC 4 cut(s) 96, 144, 244, 262
GlaI GCGC 2 cut(s) 18, 423
GluI GCNGC 4 cut(s) 96, 144, 244, 262
HaeIII GGCC 4 cut(s) 77, 98, 235, 324
HapII CCGG 3 cut(s) 121, 211, 326
HhaI GCGC 2 cut(s) 19, 424
Hin1I GRCGYC 2 cut(s) 162, 186
Hin1II CATG 1 cut(s) 269
Hin6I GCGC 2 cut(s) 17, 422
HinP1I GCGC 2 cut(s) 17, 422
HincII GTYRAC 2 cut(s) 113, 137
HindII GTYRAC 2 cut(s) 113, 137
HinfI GANTC 7 cut(s) 63, 109, 114, 133, 149, 299, 338
HpaII CCGG 3 cut(s) 121, 211, 326
HphI GGTGA 2 cut(s) 27, 376
Hpy166II GTNNAC 3 cut(s) 11, 113, 137
Hpy188I TCNGA 3 cut(s) 68, 175, 397
Hpy188III TCNNGA 3 cut(s) 121, 158, 359
Hpy8I GTNNAC 3 cut(s) 11, 113, 137
Hpy99I CGWCG 1 cut(s) 206
HpyAV CCTTC 1 cut(s) 361
HpyCH4III ACNGT 1 cut(s) 320
HpyCH4IV ACGT 4 cut(s) 162, 186, 201, 230
HpyCH4V TGCA 1 cut(s) 346
HpyF10VI GCNNNNNNNGC 2 cut(s) 101, 500
HpySE526I ACGT 4 cut(s) 162, 186, 201, 230
Hsp92I GRCGYC 2 cut(s) 162, 186
Hsp92II CATG 1 cut(s) 269
HspAI GCGC 2 cut(s) 17, 422
Kpn2I TCCGGA 1 cut(s) 120
KspI CCGCGG 1 cut(s) 495
LmnI GCTCC 2 cut(s) 213, 315
Lsp1109I GCAGC 2 cut(s) 248, 255
MaeII ACGT 4 cut(s) 162, 186, 201, 230
MaeIII GTNAC 2 cut(s) 250, 352
MhlI GDGCHC 1 cut(s) 210
MluCI AATT 2 cut(s) 49, 388
MlyI GAGTC 4 cut(s) 108, 118, 142, 293
MmeI TCCRAC 2 cut(s) 114, 198
MnlI CCTC 8 cut(s) 43, 49, 69, 93, 109, 280, 355, 476
Mph1103I ATGCAT 1 cut(s) 348
MroI TCCGGA 1 cut(s) 120
MseI TTAA 1 cut(s) 510
MspA1I CMGCKG 1 cut(s) 494
MspI CCGG 3 cut(s) 121, 211, 326
MspR9I CCNGG 2 cut(s) 191, 326
MvaI CCWGG 1 cut(s) 191
MvnI CGCG 3 cut(s) 17, 95, 494
MwoI GCNNNNNNNGC 2 cut(s) 101, 500
NciI CCSGG 1 cut(s) 326
NlaIII CATG 1 cut(s) 269
NlaIV GGNNCC 2 cut(s) 76, 290
NmuCI GTSAC 1 cut(s) 250
NsiI ATGCAT 1 cut(s) 348
PaqCI CACCTGC 1 cut(s) 401
PfeI GAWTC 3 cut(s) 63, 149, 338
PkrI GCNGC 4 cut(s) 97, 145, 245, 263
PleI GAGTC 4 cut(s) 108, 117, 141, 293
PmaCI CACGTG 1 cut(s) 231
PmlI CACGTG 1 cut(s) 231
PpsI GAGTC 4 cut(s) 108, 117, 141, 293
Ppu21I YACGTR 1 cut(s) 231
Psp6I CCWGG 1 cut(s) 189
PspCI CACGTG 1 cut(s) 231
PspGI CCWGG 1 cut(s) 189
PspN4I GGNNCC 2 cut(s) 76, 290
PspPI GGNCC 3 cut(s) 75, 288, 323
RsaI GTAC 1 cut(s) 200
RsaNI GTAC 1 cut(s) 199
SacII CCGCGG 1 cut(s) 495
SalI GTCGAC 1 cut(s) 111
SaqAI TTAA 1 cut(s) 510
SatI GCNGC 4 cut(s) 96, 144, 244, 262
Sau96I GGNCC 3 cut(s) 75, 288, 323
SchI GAGTC 4 cut(s) 108, 118, 142, 293
ScrFI CCNGG 2 cut(s) 191, 326
SduI GDGCHC 1 cut(s) 210
Sfr303I CCGCGG 1 cut(s) 495
SgrBI CCGCGG 1 cut(s) 495
SinI GGWCC 1 cut(s) 288
SmlI CTYRAG 1 cut(s) 359
SmoI CTYRAG 1 cut(s) 359
Sse9I AATT 2 cut(s) 49, 388
SsiI CCGC 7 cut(s) 95, 102, 141, 144, 279, 492, 494
StyD4I CCNGG 2 cut(s) 189, 324
TaaI ACNGT 1 cut(s) 320
TaiI ACGT 4 cut(s) 165, 189, 204, 233
TaqI TCGA 6 cut(s) 61, 112, 128, 225, 336, 405
TasI AATT 2 cut(s) 49, 388
TauI GCSGC 2 cut(s) 98, 146
TfiI GAWTC 3 cut(s) 63, 149, 338
Tru1I TTAA 1 cut(s) 510
Tru9I TTAA 1 cut(s) 510
TseFI GTSAC 1 cut(s) 250
TseI GCWGC 2 cut(s) 243, 261
Tsp45I GTSAC 1 cut(s) 250
TspGWI ACGGA 3 cut(s) 75, 197, 262
VpaK11BI GGWCC 1 cut(s) 288
XapI RAATTY 2 cut(s) 49, 388
XmiI GTMKAC 2 cut(s) 10, 112
ZraI GACGTC 2 cut(s) 163, 187
Zsp2I ATGCAT 1 cut(s) 348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.