FvH4_2g36680

peptidyl-tRNA hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0010251
Physical Location & Seq
Reverse (-)
26808675 .. 26811703
3029 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g36680.t1

Sequence Viewer

Length: 519 bp
ATGATGGCCTGTATTGTATCGCAATTTCGTTTCGCTCATCTCCCAAGCACCCGAGTTCGTTTTGGGCCGAAACCTACACTCACCGCCCCGATTCGACTCGGATTCAGCTCAATGTCTCAACCGGCATCGGAAGCCAATCCGGACACCTCGAAACCGGAGACCCCCGATGTCGTCGTTCAGTACGTGGTGCTCCGGCGAGACCTAATCGACACGTGGCCGTTAGGCAGTGTGGTGACGCAGGGCTGCCATGCCTCCGTCTCCGCCATCTGGTCCCACAAAGAGGACCCTCACACGCTCCAGTACTGCAGCCCTGAATCCATTGATTCTATGCATAAGGTTACTCTTGAGGTGAAGGGAGAACCCCAAATCTTGAACTTGTCTGAGAAGCTTAAAGCTGGTGGAATAGAGCATAAACTGTGGATTGAGCAACCTGAGAACTTCCCAACATGTCTCGCTACGAAGCCTTACCCCAAATCCATGGTATCTTCGTATTTCAAAAAGTTGAAACTCTGTAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTH2

Protein Analysis

173

Amino Acids

19.22

Weight (kDa)

8.8

Isoelectric Point (pI)

51.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTH2 PF01981 59 - 171 1.5e-24 Peptidyl-tRNA hydrolase PTH2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010251)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10700 AT5G10700
fragaria_vesca FvH4_2g36680
malus_domestica MD08G1127800.v1.1 MD15G1107200.v1.1
prunus_persica Prupe.1G459500_v2.0.a1
pyrus_communis pycom08g10680 pycom15g09800
rosa_chinensis RchiOBHm_Chr1g0366021 RchiOBHm_Chr6g0305051
rosa_laevigata RLG00000010923
rosa_multiflora Rmu_sc0017427.1_g000001
rosa_roxburghii Rroxscaffold_7G00163200
rosa_rugosa Rorug06G0337000
rosa_samantha Rh1AG334900 Rh6AG448600 Rh6BG452300 Rh6CG462300 Rh6DG449200
rosa_wichuraiana Rw1G029710 Rw6G039080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 139
AciI CCGC 2 cut(s) 84, 261
AcoI YGGCCR 1 cut(s) 215
AcvI CACGTG 1 cut(s) 213
AfaI GTAC 2 cut(s) 182, 302
AfiI CCNNNNNNNGG 2 cut(s) 267, 280
AflIII ACRYGT 2 cut(s) 210, 446
AgsI TTSAA 3 cut(s) 373, 496, 505
AluBI AGCT 3 cut(s) 108, 388, 395
AluI AGCT 3 cut(s) 108, 388, 395
Alw21I GWGCWC 1 cut(s) 192
Alw26I GTCTC 5 cut(s) 120, 152, 192, 262, 455
Ama87I CYCGRG 1 cut(s) 51
Aor13HI TCCGGA 1 cut(s) 139
AoxI GGCC 3 cut(s) 6, 65, 215
ApeKI GCWGC 2 cut(s) 243, 306
AspS9I GGNCC 3 cut(s) 65, 270, 283
AsuHPI GGTGA 3 cut(s) 73, 244, 361
AvaI CYCGRG 1 cut(s) 51
AvaII GGWCC 2 cut(s) 270, 283
BbrPI CACGTG 1 cut(s) 213
Bbv12I GWGCWC 1 cut(s) 192
BbvI GCAGC 2 cut(s) 230, 318
BccI CCATC 1 cut(s) 272
BceAI ACGGC 1 cut(s) 202
BcoDI GTCTC 5 cut(s) 120, 152, 192, 262, 455
BfmI CTRYAG 1 cut(s) 304
BisI GCNGC 2 cut(s) 244, 307
BlsI GCNGC 2 cut(s) 245, 308
BmcAI AGTACT 1 cut(s) 302
Bme18I GGWCC 2 cut(s) 270, 283
BmeT110I CYCGRG 1 cut(s) 51
BmgT120I GGNCC 3 cut(s) 65, 270, 283
BmiI GGNNCC 2 cut(s) 272, 285
BmsI GCATC 1 cut(s) 134
BpmI CTGGAG 1 cut(s) 281
BpuEI CTTGAG 1 cut(s) 365
BsaAI YACGTR 2 cut(s) 184, 213
BsaI GGTCTC 2 cut(s) 152, 192
BsaJI CCNNGG 1 cut(s) 477
BsaWI WCCGGW 2 cut(s) 139, 154
Bsc4I CCNNNNNNNGG 2 cut(s) 267, 280
Bse118I RCCGGY 1 cut(s) 121
Bse1I ACTGG 1 cut(s) 298
BseAI TCCGGA 1 cut(s) 139
BseDI CCNNGG 1 cut(s) 477
BseLI CCNNNNNNNGG 2 cut(s) 267, 280
BseMII CTCAG 2 cut(s) 372, 423
BseNI ACTGG 1 cut(s) 298
BseXI GCAGC 2 cut(s) 230, 318
BshFI GGCC 3 cut(s) 8, 67, 217
BsiHKAI GWGCWC 1 cut(s) 192
BsiHKCI CYCGRG 1 cut(s) 51
BsiSI CCGG 4 cut(s) 122, 140, 155, 193
BslFI GGGAC 1 cut(s) 256
BslI CCNNNNNNNGG 2 cut(s) 267, 280
BsmAI GTCTC 5 cut(s) 120, 152, 192, 262, 455
BsmBI CGTCTC 1 cut(s) 262
BsmFI GGGAC 1 cut(s) 256
BsnI GGCC 3 cut(s) 8, 67, 217
Bso31I GGTCTC 2 cut(s) 152, 192
BsoBI CYCGRG 1 cut(s) 51
Bsp1286I GDGCHC 1 cut(s) 192
Bsp13I TCCGGA 1 cut(s) 139
Bsp19I CCATGG 1 cut(s) 477
BspACI CCGC 2 cut(s) 84, 261
BspANI GGCC 3 cut(s) 8, 67, 217
BspCNI CTCAG 2 cut(s) 373, 424
BspEI TCCGGA 1 cut(s) 139
BspLI GGNNCC 2 cut(s) 272, 285
BspMAI CTGCAG 1 cut(s) 308
BspTNI GGTCTC 2 cut(s) 152, 192
BsrFI RCCGGY 1 cut(s) 121
BsrI ACTGG 1 cut(s) 298
BssAI RCCGGY 1 cut(s) 121
BssECI CCNNGG 1 cut(s) 477
BssT1I CCWWGG 1 cut(s) 477
Bst4CI ACNGT 1 cut(s) 417
BstBAI YACGTR 2 cut(s) 184, 213
BstDEI CTNAG 2 cut(s) 381, 432
BstDSI CCRYGG 1 cut(s) 477
BstMAI GTCTC 5 cut(s) 120, 152, 192, 262, 455
BstMWI GCNNNNNNNGC 1 cut(s) 131
BstNSI RCATGY 1 cut(s) 450
BstSFI CTRYAG 1 cut(s) 304
BstV1I GCAGC 2 cut(s) 230, 318
BstXI CCANNNNNNTGG 1 cut(s) 478
BsuRI GGCC 3 cut(s) 8, 67, 217
BtgI CCRYGG 1 cut(s) 477
BtsI GCAGTG 1 cut(s) 232
BtsIMutI CAGTG 1 cut(s) 232
Cfr10I RCCGGY 1 cut(s) 121
Cfr13I GGNCC 3 cut(s) 65, 270, 283
CseI GACGC 1 cut(s) 244
Csp6I GTAC 2 cut(s) 181, 301
CviAII CATG 3 cut(s) 248, 447, 478
CviQI GTAC 2 cut(s) 181, 301
DdeI CTNAG 2 cut(s) 381, 432
EaeI YGGCCR 1 cut(s) 215
EciI GGCGGA 1 cut(s) 250
Eco130I CCWWGG 1 cut(s) 477
Eco31I GGTCTC 2 cut(s) 152, 192
Eco47I GGWCC 2 cut(s) 270, 283
Eco72I CACGTG 1 cut(s) 213
Eco88I CYCGRG 1 cut(s) 51
EcoO109I RGGNCCY 1 cut(s) 283
EcoT14I CCWWGG 1 cut(s) 477
EcoT22I ATGCAT 1 cut(s) 333
ErhI CCWWGG 1 cut(s) 477
Esp3I CGTCTC 1 cut(s) 262
FaeI CATG 3 cut(s) 251, 450, 481
FaiI YATR 6 cut(s) 249, 329, 333, 411, 448, 479
FaqI GGGAC 1 cut(s) 256
FatI CATG 3 cut(s) 247, 446, 477
Fnu4HI GCNGC 2 cut(s) 244, 307
Fsp4HI GCNGC 2 cut(s) 244, 307
GluI GCNGC 2 cut(s) 244, 307
GsuI CTGGAG 1 cut(s) 281
HaeIII GGCC 3 cut(s) 8, 67, 217
HapII CCGG 4 cut(s) 122, 140, 155, 193
HgaI GACGC 1 cut(s) 244
Hin1II CATG 3 cut(s) 251, 450, 481
HindIII AAGCTT 1 cut(s) 386
HinfI GANTC 5 cut(s) 91, 96, 102, 314, 323
HpaII CCGG 4 cut(s) 122, 140, 155, 193
HphI GGTGA 3 cut(s) 73, 244, 361
Hpy188I TCNGA 3 cut(s) 101, 130, 382
Hpy188III TCNNGA 3 cut(s) 140, 344, 370
Hpy99I CGWCG 1 cut(s) 176
HpyAV CCTTC 1 cut(s) 346
HpyCH4III ACNGT 1 cut(s) 417
HpyCH4IV ACGT 2 cut(s) 183, 212
HpyCH4V TGCA 2 cut(s) 306, 331
HpyF10VI GCNNNNNNNGC 1 cut(s) 131
HpyF3I CTNAG 2 cut(s) 381, 432
HpySE526I ACGT 2 cut(s) 183, 212
Hsp92II CATG 3 cut(s) 251, 450, 481
Kpn2I TCCGGA 1 cut(s) 139
LmnI GCTCC 2 cut(s) 195, 300
Lsp1109I GCAGC 2 cut(s) 230, 318
LweI GCATC 1 cut(s) 134
MaeII ACGT 2 cut(s) 183, 212
MaeIII GTNAC 2 cut(s) 232, 337
MboII GAAGA 1 cut(s) 477
MhlI GDGCHC 1 cut(s) 192
MluCI AATT 1 cut(s) 23
MlyI GAGTC 1 cut(s) 90
MnlI CCTC 5 cut(s) 157, 262, 274, 297, 340
Mph1103I ATGCAT 1 cut(s) 333
MroI TCCGGA 1 cut(s) 139
MseI TTAA 1 cut(s) 390
MspI CCGG 4 cut(s) 122, 140, 155, 193
MwoI GCNNNNNNNGC 1 cut(s) 131
NcoI CCATGG 1 cut(s) 477
NlaIII CATG 3 cut(s) 251, 450, 481
NlaIV GGNNCC 2 cut(s) 272, 285
NmuCI GTSAC 1 cut(s) 232
NsiI ATGCAT 1 cut(s) 333
NspI RCATGY 1 cut(s) 450
PciI ACATGT 1 cut(s) 446
PcsI WCGNNNNNNNCGW 1 cut(s) 180
PfeI GAWTC 4 cut(s) 91, 102, 314, 323
PkrI GCNGC 2 cut(s) 245, 308
PleI GAGTC 1 cut(s) 90
PmaCI CACGTG 1 cut(s) 213
PmlI CACGTG 1 cut(s) 213
PpsI GAGTC 1 cut(s) 90
Ppu21I YACGTR 2 cut(s) 184, 213
PpuMI RGGWCCY 1 cut(s) 283
PscI ACATGT 1 cut(s) 446
Psp5II RGGWCCY 1 cut(s) 283
PspCI CACGTG 1 cut(s) 213
PspN4I GGNNCC 2 cut(s) 272, 285
PspPI GGNCC 3 cut(s) 65, 270, 283
PspPPI RGGWCCY 1 cut(s) 283
PstI CTGCAG 1 cut(s) 308
RsaI GTAC 2 cut(s) 182, 302
RsaNI GTAC 2 cut(s) 181, 301
SaqAI TTAA 1 cut(s) 390
SatI GCNGC 2 cut(s) 244, 307
Sau96I GGNCC 3 cut(s) 65, 270, 283
ScaI AGTACT 1 cut(s) 302
SchI GAGTC 1 cut(s) 90
SduI GDGCHC 1 cut(s) 192
SfaNI GCATC 1 cut(s) 134
SfcI CTRYAG 1 cut(s) 304
SinI GGWCC 2 cut(s) 270, 283
SmlI CTYRAG 1 cut(s) 344
SmoI CTYRAG 1 cut(s) 344
Sse9I AATT 1 cut(s) 23
SsiI CCGC 2 cut(s) 84, 261
StyI CCWWGG 1 cut(s) 477
TaaI ACNGT 1 cut(s) 417
TaiI ACGT 2 cut(s) 186, 215
TaqI TCGA 3 cut(s) 94, 149, 207
TasI AATT 1 cut(s) 23
TatI WGTACW 1 cut(s) 300
TfiI GAWTC 4 cut(s) 91, 102, 314, 323
Tru1I TTAA 1 cut(s) 390
Tru9I TTAA 1 cut(s) 390
TscAI CASTG 1 cut(s) 232
TseFI GTSAC 1 cut(s) 232
TseI GCWGC 2 cut(s) 243, 306
Tsp45I GTSAC 1 cut(s) 232
TspGWI ACGGA 1 cut(s) 244
TspRI CASTG 1 cut(s) 232
VpaK11BI GGWCC 2 cut(s) 270, 283
XceI RCATGY 1 cut(s) 450
ZrmI AGTACT 1 cut(s) 302
Zsp2I ATGCAT 1 cut(s) 333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.