Rw1G029710

Peptidyl-tRNA hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0010251
Physical Location & Seq
Reverse (-)
57394594 .. 57396449
1856 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G029710.1

Sequence Viewer

Length: 426 bp
ATGTCTCAACCGGCCTCGGAAGCCAATCCGGACACCTCGAAACCCGAGACCCCTGACGTCCTCGTTCAGTACGTGGTGCTCCGGCGAGACCTAATCGACACGTGGCCGTTGGGCAGTGTGGTAACACAGGGCTGCCATGCCTCCGTCTCCGCCACCTGGTCCCACAAAGACGACCCACACACGCTCCAGTATTGTAGCCCTGAAAATATCGATTCTATGCATAAGGTTACTCTTGAGGTGAAGGGAGAACCTCAAATATTGAACTTGTCTGAGAAGCTCAAAGCTGGTGGCATAGAGCACAAACTGTGGATTGAGCAACCTGAGAATTTCCCAACTTGTCTCGCTACGAAACCTTACCCCAAATCCATGTTTTGCTTTCCCATTGTGTCGAAAGTGTTAGAATATAGTATTGGGATGGCCAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTH2

Protein Analysis

141

Amino Acids

15.72

Weight (kDa)

5.68

Isoelectric Point (pI)

54.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTH2 PF01981 22 - 123 2.3e-20 Peptidyl-tRNA hydrolase PTH2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010251)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10700 AT5G10700
fragaria_vesca FvH4_2g36680
malus_domestica MD08G1127800.v1.1 MD15G1107200.v1.1
prunus_persica Prupe.1G459500_v2.0.a1
pyrus_communis pycom08g10680 pycom15g09800
rosa_chinensis RchiOBHm_Chr1g0366021 RchiOBHm_Chr6g0305051
rosa_laevigata RLG00000010923
rosa_multiflora Rmu_sc0017427.1_g000001
rosa_roxburghii Rroxscaffold_7G00163200
rosa_rugosa Rorug06G0337000
rosa_samantha Rh1AG334900 Rh6AG448600 Rh6BG452300 Rh6CG462300 Rh6DG449200
rosa_wichuraiana Rw1G029710 Rw6G039080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 60
AccIII TCCGGA 1 cut(s) 28
AciI CCGC 1 cut(s) 150
AcoI YGGCCR 2 cut(s) 104, 417
AcsI RAATTY 1 cut(s) 325
AcvI CACGTG 1 cut(s) 102
AcyI GRCGYC 1 cut(s) 57
AfaI GTAC 1 cut(s) 71
AfiI CCNNNNNNNGG 1 cut(s) 156
AflIII ACRYGT 1 cut(s) 99
AgsI TTSAA 1 cut(s) 262
AjnI CCWGG 1 cut(s) 155
AjuI GAANNNNNNNTTGG 2 cut(s) 393, 425
AluBI AGCT 2 cut(s) 277, 284
AluI AGCT 2 cut(s) 277, 284
Alw21I GWGCWC 2 cut(s) 81, 300
Alw26I GTCTC 5 cut(s) 9, 41, 81, 151, 344
Ama87I CYCGRG 1 cut(s) 44
Aor13HI TCCGGA 1 cut(s) 28
AoxI GGCC 3 cut(s) 12, 104, 417
ApeKI GCWGC 1 cut(s) 132
ApoI RAATTY 1 cut(s) 325
AspS9I GGNCC 1 cut(s) 159
AsuHPI GGTGA 1 cut(s) 250
AvaI CYCGRG 1 cut(s) 44
AvaII GGWCC 1 cut(s) 159
BalI TGGCCA 1 cut(s) 419
BbrPI CACGTG 1 cut(s) 102
Bbv12I GWGCWC 2 cut(s) 81, 300
BbvI GCAGC 1 cut(s) 119
BccI CCATC 1 cut(s) 409
BceAI ACGGC 1 cut(s) 91
BciT130I CCWGG 1 cut(s) 157
BcoDI GTCTC 5 cut(s) 9, 41, 81, 151, 344
BisI GCNGC 1 cut(s) 133
BlsI GCNGC 1 cut(s) 134
Bme1390I CCNGG 1 cut(s) 157
Bme18I GGWCC 1 cut(s) 159
BmeT110I CYCGRG 1 cut(s) 44
BmgT120I GGNCC 1 cut(s) 159
BmiI GGNNCC 1 cut(s) 161
BmrFI CCNGG 1 cut(s) 157
BpmI CTGGAG 1 cut(s) 170
BpuEI CTTGAG 1 cut(s) 254
Bsa29I ATCGAT 1 cut(s) 210
BsaAI YACGTR 2 cut(s) 73, 102
BsaHI GRCGYC 1 cut(s) 57
BsaI GGTCTC 2 cut(s) 41, 81
BsaJI CCNNGG 1 cut(s) 15
BsaWI WCCGGW 1 cut(s) 28
BsaXI ACNNNNNCTCC 2 cut(s) 168, 198
Bsc4I CCNNNNNNNGG 1 cut(s) 156
Bse118I RCCGGY 1 cut(s) 10
Bse1I ACTGG 1 cut(s) 187
BseAI TCCGGA 1 cut(s) 28
BseBI CCWGG 1 cut(s) 157
BseCI ATCGAT 1 cut(s) 210
BseDI CCNNGG 1 cut(s) 15
BseGI GGATG 1 cut(s) 420
BseLI CCNNNNNNNGG 1 cut(s) 156
BseMII CTCAG 2 cut(s) 261, 312
BseNI ACTGG 1 cut(s) 187
BseXI GCAGC 1 cut(s) 119
BshFI GGCC 3 cut(s) 14, 106, 419
BshVI ATCGAT 1 cut(s) 210
BsiHKAI GWGCWC 2 cut(s) 81, 300
BsiHKCI CYCGRG 1 cut(s) 44
BsiSI CCGG 3 cut(s) 11, 29, 82
BslFI GGGAC 1 cut(s) 145
BslI CCNNNNNNNGG 1 cut(s) 156
BsmAI GTCTC 5 cut(s) 9, 41, 81, 151, 344
BsmBI CGTCTC 1 cut(s) 151
BsmFI GGGAC 1 cut(s) 145
BsnI GGCC 3 cut(s) 14, 106, 419
Bso31I GGTCTC 2 cut(s) 41, 81
BsoBI CYCGRG 1 cut(s) 44
Bsp1286I GDGCHC 2 cut(s) 81, 300
Bsp13I TCCGGA 1 cut(s) 28
BspACI CCGC 1 cut(s) 150
BspANI GGCC 3 cut(s) 14, 106, 419
BspCNI CTCAG 2 cut(s) 262, 313
BspDI ATCGAT 1 cut(s) 210
BspEI TCCGGA 1 cut(s) 28
BspLI GGNNCC 1 cut(s) 161
BspTNI GGTCTC 2 cut(s) 41, 81
BsrFI RCCGGY 1 cut(s) 10
BsrI ACTGG 1 cut(s) 187
BssAI RCCGGY 1 cut(s) 10
BssECI CCNNGG 1 cut(s) 15
BssNI GRCGYC 1 cut(s) 57
Bst2UI CCWGG 1 cut(s) 157
Bst4CI ACNGT 1 cut(s) 306
BstACI GRCGYC 1 cut(s) 57
BstBAI YACGTR 2 cut(s) 73, 102
BstDEI CTNAG 2 cut(s) 270, 321
BstF5I GGATG 1 cut(s) 420
BstMAI GTCTC 5 cut(s) 9, 41, 81, 151, 344
BstMWI GCNNNNNNNGC 1 cut(s) 20
BstNI CCWGG 1 cut(s) 157
BstSCI CCNGG 1 cut(s) 155
BstV1I GCAGC 1 cut(s) 119
Bsu15I ATCGAT 1 cut(s) 210
BsuRI GGCC 3 cut(s) 14, 106, 419
BsuTUI ATCGAT 1 cut(s) 210
BtsCI GGATG 1 cut(s) 420
BtsI GCAGTG 1 cut(s) 121
BtsIMutI CAGTG 1 cut(s) 121
Cfr10I RCCGGY 1 cut(s) 10
Cfr13I GGNCC 1 cut(s) 159
ClaI ATCGAT 1 cut(s) 210
CsiI ACCWGGT 1 cut(s) 155
Csp6I GTAC 1 cut(s) 70
CspCI CAANNNNNGTGG 2 cut(s) 268, 303
CviAII CATG 2 cut(s) 137, 367
CviJI RGCY 8 cut(s) 14, 23, 106, 132, 198, 277, 284, 419
CviKI_1 RGCY 8 cut(s) 14, 23, 106, 132, 198, 277, 284, 419
CviQI GTAC 1 cut(s) 70
DdeI CTNAG 2 cut(s) 270, 321
EaeI YGGCCR 2 cut(s) 104, 417
EciI GGCGGA 1 cut(s) 139
Eco31I GGTCTC 2 cut(s) 41, 81
Eco47I GGWCC 1 cut(s) 159
Eco72I CACGTG 1 cut(s) 102
Eco88I CYCGRG 1 cut(s) 44
EcoRII CCWGG 1 cut(s) 155
EcoT22I ATGCAT 1 cut(s) 222
Esp3I CGTCTC 1 cut(s) 151
FaeI CATG 2 cut(s) 140, 370
FaiI YATR 6 cut(s) 138, 218, 222, 293, 368, 405
FaqI GGGAC 1 cut(s) 145
FatI CATG 2 cut(s) 136, 366
Fnu4HI GCNGC 1 cut(s) 133
Fsp4HI GCNGC 1 cut(s) 133
GluI GCNGC 1 cut(s) 133
GsuI CTGGAG 1 cut(s) 170
HaeIII GGCC 3 cut(s) 14, 106, 419
HapII CCGG 3 cut(s) 11, 29, 82
Hin1I GRCGYC 1 cut(s) 57
Hin1II CATG 2 cut(s) 140, 370
HinfI GANTC 1 cut(s) 212
HpaII CCGG 3 cut(s) 11, 29, 82
HphI GGTGA 1 cut(s) 250
Hpy188I TCNGA 2 cut(s) 19, 271
Hpy188III TCNNGA 2 cut(s) 29, 233
HpyAV CCTTC 1 cut(s) 235
HpyCH4III ACNGT 1 cut(s) 306
HpyCH4IV ACGT 3 cut(s) 57, 72, 101
HpyCH4V TGCA 1 cut(s) 220
HpyF10VI GCNNNNNNNGC 1 cut(s) 20
HpyF3I CTNAG 2 cut(s) 270, 321
HpySE526I ACGT 3 cut(s) 57, 72, 101
Hsp92I GRCGYC 1 cut(s) 57
Hsp92II CATG 2 cut(s) 140, 370
Kpn2I TCCGGA 1 cut(s) 28
LmnI GCTCC 2 cut(s) 84, 189
Lsp1109I GCAGC 1 cut(s) 119
MabI ACCWGGT 1 cut(s) 155
MaeII ACGT 3 cut(s) 57, 72, 101
MaeIII GTNAC 2 cut(s) 121, 226
MhlI GDGCHC 2 cut(s) 81, 300
MlsI TGGCCA 1 cut(s) 419
MluCI AATT 1 cut(s) 325
MluNI TGGCCA 1 cut(s) 419
MnlI CCTC 6 cut(s) 25, 46, 71, 151, 229, 261
Mox20I TGGCCA 1 cut(s) 419
Mph1103I ATGCAT 1 cut(s) 222
MroI TCCGGA 1 cut(s) 28
MscI TGGCCA 1 cut(s) 419
Msp20I TGGCCA 1 cut(s) 419
MspI CCGG 3 cut(s) 11, 29, 82
MspR9I CCNGG 1 cut(s) 157
MvaI CCWGG 1 cut(s) 157
MwoI GCNNNNNNNGC 1 cut(s) 20
NlaIII CATG 2 cut(s) 140, 370
NlaIV GGNNCC 1 cut(s) 161
NsiI ATGCAT 1 cut(s) 222
PcsI WCGNNNNNNNCGW 1 cut(s) 69
PfeI GAWTC 1 cut(s) 212
PkrI GCNGC 1 cut(s) 134
PmaCI CACGTG 1 cut(s) 102
PmlI CACGTG 1 cut(s) 102
Ppu21I YACGTR 2 cut(s) 73, 102
Psp6I CCWGG 1 cut(s) 155
PspCI CACGTG 1 cut(s) 102
PspGI CCWGG 1 cut(s) 155
PspN4I GGNNCC 1 cut(s) 161
PspPI GGNCC 1 cut(s) 159
RsaI GTAC 1 cut(s) 71
RsaNI GTAC 1 cut(s) 70
SatI GCNGC 1 cut(s) 133
Sau96I GGNCC 1 cut(s) 159
ScrFI CCNGG 1 cut(s) 157
SduI GDGCHC 2 cut(s) 81, 300
SexAI ACCWGGT 1 cut(s) 155
SinI GGWCC 1 cut(s) 159
SmlI CTYRAG 1 cut(s) 233
SmoI CTYRAG 1 cut(s) 233
Sse9I AATT 1 cut(s) 325
SsiI CCGC 1 cut(s) 150
SspI AATATT 1 cut(s) 258
StyD4I CCNGG 1 cut(s) 155
TaaI ACNGT 1 cut(s) 306
TaiI ACGT 3 cut(s) 60, 75, 104
TaqI TCGA 4 cut(s) 38, 96, 210, 389
TasI AATT 1 cut(s) 325
TfiI GAWTC 1 cut(s) 212
TscAI CASTG 1 cut(s) 121
TseI GCWGC 1 cut(s) 132
TspGWI ACGGA 1 cut(s) 133
TspRI CASTG 1 cut(s) 121
VpaK11BI GGWCC 1 cut(s) 159
XapI RAATTY 1 cut(s) 325
ZraI GACGTC 1 cut(s) 58
Zsp2I ATGCAT 1 cut(s) 222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.