AT5G10730

NmrA-like family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0012254
Physical Location & Seq
Reverse (-)
3390614 .. 3393191
2578 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G10730.1

Sequence Viewer

Length: 864 bp
ATGAGGACAATCGTTTCGCGTTTGATTCGTTATCAATCGTCTCTTTCCCAGATACGGTTTGTTTCTGCCTCTGGTGGCGGGAGGTATCTCTCGACAGACTCTAACAAAATTGATGAGCCCTTCAATGTGGAAGAAGCTGAGACTGTACATGTGCCTCCACCTCCTACTGAGAAGCTGCTTGTCCTGGGAGGAAATGGTTTTGTGGGATCACATGTCTGTAAAGAAGCTTTAGATCGCGGCTTATCTGTCTCTAGCCTTAGCAGGTCAGGTAGGTCGTCTTTACAAGAGTCCTGGGCCTCCAGAGTAACATGGCATCAAGGAAACCTTCTATCATCTGATTTATTGAAAGATGCTCTTGAGGGAGTGACTTCTGTGATTTCTTGTGTTGGTGGTTTTGGTTCAAACTCATATATGTATAAGATTAACGGGACTGCAAACATCAACGCAATTAGGGCTGCCTCAGAAAAAGGTGTTAAAAGATTTGTCTATATTTCTGCTGCGGATTTCGGATTAGCCAACTACTTGTTGAGAGGATACTATGAGGGAAAGCGAGCTGCTGAGACCGAGCTGCTCACAAGATTTGCATATGGAGGTATAATCTTGCGACCTGGTTTTATATATGGAACTCGCAGTGTTGGGAGCATGAAGATCCCGTTAGGAGTATTTGGTTCACCCATGGAGATGGTTCTTCAACAAGCAAAACCTCTGAACCAGCTTCCATTAGTCGGACCTTTGTTCACACCTCCGGTGAATGTTGAATCGGTTGCAAAAGTCGCAGTTAGAGCAGCCACAGATCCAGTGTTCCCTCCCGGGATTGTGGATGTCCATGGAATACAACGTTACAGCCAACAGAAATCGAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAD_binding_10

Protein Analysis

287

Amino Acids

31.04

Weight (kDa)

9.58

Isoelectric Point (pI)

41.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 56 - 165 4.6e-11 NmrA-like family
Epimerase PF01370 60 - 167 1.6e-14 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 60 - 170 1.2e-10 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_10 PF13460 63 - 206 5.6e-19 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012254)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10730
fragaria_vesca FvH4_2g41440 FvH4_2g41440
malus_domestica MD08G1049300.v1.1 MD15G1045600.v1.1
prunus_persica Prupe.1G399300_v2.0.a1
pyrus_communis pycom08g04120 pycom15g04250
rosa_chinensis RchiOBHm_Chr6g0299041
rosa_laevigata RLG00000011467
rosa_multiflora Rmu_sc0000981.1_g000004 Rmu_sc0032629.1_g000004
rosa_roxburghii Rroxscaffold_7G00169060
rosa_rugosa Rorug06G0284300
rosa_samantha Rh6AG395800 Rh6BG404400 Rh6CG410300 Rh6DG397000
rosa_wichuraiana Rw6G034640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 252
AccII CGCG 2 cut(s) 19, 237
AciI CCGC 3 cut(s) 78, 237, 500
AclI AACGTT 1 cut(s) 838
AclWI GGATC 3 cut(s) 214, 643, 788
AfaI GTAC 1 cut(s) 147
AfiI CCNNNNNNNGG 2 cut(s) 54, 725
AflIII ACRYGT 2 cut(s) 148, 211
AgsI TTSAA 5 cut(s) 124, 346, 402, 692, 758
AjnI CCWGG 3 cut(s) 183, 290, 607
AluBI AGCT 6 cut(s) 137, 175, 227, 554, 568, 715
AluI AGCT 6 cut(s) 137, 175, 227, 554, 568, 715
Alw26I GTCTC 4 cut(s) 45, 134, 253, 554
AlwI GGATC 3 cut(s) 214, 643, 788
Ama87I CYCGRG 1 cut(s) 809
AoxI GGCC 1 cut(s) 294
ApeKI GCWGC 6 cut(s) 175, 455, 497, 554, 568, 785
ArsI GACNNNNNNTTYG 1 cut(s) 30
AspS9I GGNCC 2 cut(s) 294, 728
AsuC2I CCSGG 2 cut(s) 810, 811
AsuHPI GGTGA 2 cut(s) 663, 760
AvaI CYCGRG 1 cut(s) 809
AvaII GGWCC 1 cut(s) 728
BanII GRGCYC 1 cut(s) 120
BbvI GCAGC 6 cut(s) 162, 442, 484, 541, 555, 797
BccI CCATC 1 cut(s) 676
BciT130I CCWGG 3 cut(s) 185, 292, 609
BciVI GTATCC 1 cut(s) 527
BcnI CCSGG 2 cut(s) 810, 811
BcoDI GTCTC 4 cut(s) 45, 134, 253, 554
BfaI CTAG 1 cut(s) 252
BfuAI ACCTGC 1 cut(s) 252
BfuI GTATCC 1 cut(s) 527
BisI GCNGC 7 cut(s) 176, 238, 456, 498, 555, 569, 786
BlsI GCNGC 7 cut(s) 177, 239, 457, 499, 556, 570, 787
Bme1390I CCNGG 5 cut(s) 185, 292, 609, 810, 811
Bme18I GGWCC 1 cut(s) 728
BmeT110I CYCGRG 1 cut(s) 809
BmgT120I GGNCC 2 cut(s) 294, 728
BmrFI CCNGG 5 cut(s) 185, 292, 609, 810, 811
BmsI GCATC 2 cut(s) 322, 340
BpmI CTGGAG 1 cut(s) 283
Bpu10I CCTNAGC 1 cut(s) 257
BpuEI CTTGAG 1 cut(s) 377
BpuMI CCSGG 2 cut(s) 810, 811
BsaI GGTCTC 1 cut(s) 554
BsaJI CCNNGG 5 cut(s) 184, 291, 675, 809, 826
BsaWI WCCGGW 1 cut(s) 745
Bsc4I CCNNNNNNNGG 2 cut(s) 54, 725
Bse1I ACTGG 1 cut(s) 797
BseBI CCWGG 3 cut(s) 185, 292, 609
BseDI CCNNGG 5 cut(s) 184, 291, 675, 809, 826
BseGI GGATG 1 cut(s) 826
BseLI CCNNNNNNNGG 2 cut(s) 54, 725
BseMII CTCAG 4 cut(s) 129, 159, 474, 549
BseNI ACTGG 1 cut(s) 797
BseXI GCAGC 6 cut(s) 162, 442, 484, 541, 555, 797
Bsh1236I CGCG 2 cut(s) 19, 237
BshFI GGCC 1 cut(s) 296
BsiHKCI CYCGRG 1 cut(s) 809
BsiSI CCGG 2 cut(s) 746, 810
BslFI GGGAC 1 cut(s) 442
BslI CCNNNNNNNGG 2 cut(s) 54, 725
BsmAI GTCTC 4 cut(s) 45, 134, 253, 554
BsmBI CGTCTC 1 cut(s) 45
BsmFI GGGAC 1 cut(s) 442
BsnI GGCC 1 cut(s) 296
Bso31I GGTCTC 1 cut(s) 554
BsoBI CYCGRG 1 cut(s) 809
Bsp1286I GDGCHC 1 cut(s) 120
Bsp1407I TGTACA 1 cut(s) 145
Bsp143I GATC 4 cut(s) 206, 232, 648, 793
Bsp19I CCATGG 2 cut(s) 675, 826
BspACI CCGC 3 cut(s) 78, 237, 500
BspANI GGCC 1 cut(s) 296
BspCNI CTCAG 4 cut(s) 130, 160, 473, 550
BspFNI CGCG 2 cut(s) 19, 237
BspMI ACCTGC 1 cut(s) 252
BspPI GGATC 3 cut(s) 214, 643, 788
BspTNI GGTCTC 1 cut(s) 554
BsrGI TGTACA 1 cut(s) 145
BsrI ACTGG 1 cut(s) 797
BssECI CCNNGG 5 cut(s) 184, 291, 675, 809, 826
BssMI GATC 4 cut(s) 206, 232, 648, 793
BssT1I CCWWGG 2 cut(s) 675, 826
Bst2UI CCWGG 3 cut(s) 185, 292, 609
Bst4CI ACNGT 2 cut(s) 57, 145
BstAUI TGTACA 1 cut(s) 145
BstC8I GCNNGC 1 cut(s) 552
BstDEI CTNAG 5 cut(s) 138, 168, 257, 460, 558
BstDSI CCRYGG 2 cut(s) 675, 826
BstF5I GGATG 1 cut(s) 826
BstFNI CGCG 2 cut(s) 19, 237
BstKTI GATC 4 cut(s) 209, 235, 651, 796
BstMAI GTCTC 4 cut(s) 45, 134, 253, 554
BstMBI GATC 4 cut(s) 206, 232, 648, 793
BstMWI GCNNNNNNNGC 3 cut(s) 452, 773, 782
BstNI CCWGG 3 cut(s) 185, 292, 609
BstNSI RCATGY 2 cut(s) 152, 215
BstSCI CCNGG 5 cut(s) 183, 290, 607, 808, 809
BstUI CGCG 2 cut(s) 19, 237
BstV1I GCAGC 6 cut(s) 162, 442, 484, 541, 555, 797
BstX2I RGATCY 2 cut(s) 648, 793
BstXI CCANNNNNNTGG 1 cut(s) 682
BstYI RGATCY 2 cut(s) 648, 793
BsuI GTATCC 1 cut(s) 527
BsuRI GGCC 1 cut(s) 296
BtgI CCRYGG 2 cut(s) 675, 826
BtsCI GGATG 1 cut(s) 826
BtsI GCAGTG 1 cut(s) 637
BtsIMutI CAGTG 2 cut(s) 637, 804
BveI ACCTGC 1 cut(s) 252
Cac8I GCNNGC 1 cut(s) 552
Cfr13I GGNCC 2 cut(s) 294, 728
Cfr9I CCCGGG 1 cut(s) 809
CsiI ACCWGGT 1 cut(s) 607
Csp6I GTAC 1 cut(s) 146
CviAII CATG 6 cut(s) 149, 212, 309, 643, 676, 827
CviQI GTAC 1 cut(s) 146
DdeI CTNAG 5 cut(s) 138, 168, 257, 460, 558
DpnI GATC 4 cut(s) 208, 234, 650, 795
DpnII GATC 4 cut(s) 206, 232, 648, 793
Eco130I CCWWGG 2 cut(s) 675, 826
Eco24I GRGCYC 1 cut(s) 120
Eco31I GGTCTC 1 cut(s) 554
Eco47I GGWCC 1 cut(s) 728
Eco88I CYCGRG 1 cut(s) 809
EcoRII CCWGG 3 cut(s) 183, 290, 607
EcoT14I CCWWGG 2 cut(s) 675, 826
EcoT38I GRGCYC 1 cut(s) 120
ErhI CCWWGG 2 cut(s) 675, 826
Esp3I CGTCTC 1 cut(s) 45
FaeI CATG 6 cut(s) 152, 215, 312, 646, 679, 830
FalI AAGNNNNNCTT 4 cut(s) 309, 341, 339, 371
FaqI GGGAC 1 cut(s) 442
FatI CATG 6 cut(s) 148, 211, 308, 642, 675, 826
FauI CCCGC 1 cut(s) 71
FauNDI CATATG 1 cut(s) 586
Fnu4HI GCNGC 7 cut(s) 176, 238, 456, 498, 555, 569, 786
FokI GGATG 1 cut(s) 833
FriOI GRGCYC 1 cut(s) 120
Fsp4HI GCNGC 7 cut(s) 176, 238, 456, 498, 555, 569, 786
FspBI CTAG 1 cut(s) 252
GluI GCNGC 7 cut(s) 176, 238, 456, 498, 555, 569, 786
GsuI CTGGAG 1 cut(s) 283
HaeIII GGCC 1 cut(s) 296
HapII CCGG 2 cut(s) 746, 810
Hin1II CATG 6 cut(s) 152, 215, 312, 646, 679, 830
HindIII AAGCTT 1 cut(s) 225
HinfI GANTC 4 cut(s) 25, 98, 287, 758
HpaII CCGG 2 cut(s) 746, 810
HphI GGTGA 2 cut(s) 663, 760
Hpy166II GTNNAC 2 cut(s) 671, 738
Hpy188I TCNGA 5 cut(s) 337, 463, 509, 708, 728
Hpy188III TCNNGA 4 cut(s) 91, 300, 356, 858
Hpy8I GTNNAC 2 cut(s) 671, 738
HpyAV CCTTC 2 cut(s) 130, 335
HpyCH4III ACNGT 2 cut(s) 57, 145
HpyCH4IV ACGT 1 cut(s) 838
HpyCH4V TGCA 3 cut(s) 434, 584, 767
HpyF10VI GCNNNNNNNGC 3 cut(s) 452, 773, 782
HpyF3I CTNAG 5 cut(s) 138, 168, 257, 460, 558
HpySE526I ACGT 1 cut(s) 838
Hsp92II CATG 6 cut(s) 152, 215, 312, 646, 679, 830
Kzo9I GATC 4 cut(s) 206, 232, 648, 793
LmnI GCTCC 1 cut(s) 639
Lsp1109I GCAGC 6 cut(s) 162, 442, 484, 541, 555, 797
LweI GCATC 2 cut(s) 322, 340
MabI ACCWGGT 1 cut(s) 607
MaeI CTAG 1 cut(s) 252
MaeII ACGT 1 cut(s) 838
MaeIII GTNAC 3 cut(s) 304, 364, 839
MalI GATC 4 cut(s) 208, 234, 650, 795
MboI GATC 4 cut(s) 206, 232, 648, 793
MboII GAAGA 3 cut(s) 143, 658, 680
MflI RGATCY 2 cut(s) 648, 793
MhlI GDGCHC 1 cut(s) 120
MluCI AATT 2 cut(s) 108, 447
MlyI GAGTC 2 cut(s) 92, 296
MmeI TCCRAC 1 cut(s) 706
MseI TTAA 2 cut(s) 423, 474
MslI CAYNNNNRTG 1 cut(s) 680
MspI CCGG 2 cut(s) 746, 810
MspR9I CCNGG 5 cut(s) 185, 292, 609, 810, 811
MvaI CCWGG 3 cut(s) 185, 292, 609
MvnI CGCG 2 cut(s) 19, 237
MwoI GCNNNNNNNGC 3 cut(s) 452, 773, 782
NciI CCSGG 2 cut(s) 810, 811
NcoI CCATGG 2 cut(s) 675, 826
NdeI CATATG 1 cut(s) 586
NdeII GATC 4 cut(s) 206, 232, 648, 793
NlaIII CATG 6 cut(s) 152, 215, 312, 646, 679, 830
NmuCI GTSAC 1 cut(s) 364
NspI RCATGY 2 cut(s) 152, 215
PciI ACATGT 2 cut(s) 148, 211
PfeI GAWTC 2 cut(s) 25, 758
PkrI GCNGC 7 cut(s) 177, 239, 457, 499, 556, 570, 787
PleI GAGTC 2 cut(s) 92, 295
PpsI GAGTC 2 cut(s) 92, 295
PscI ACATGT 2 cut(s) 148, 211
Psp1406I AACGTT 1 cut(s) 838
Psp6I CCWGG 3 cut(s) 183, 290, 607
PspGI CCWGG 3 cut(s) 183, 290, 607
PspPI GGNCC 2 cut(s) 294, 728
PsuI RGATCY 2 cut(s) 648, 793
RsaI GTAC 1 cut(s) 147
RsaNI GTAC 1 cut(s) 146
RseI CAYNNNNRTG 1 cut(s) 680
SaqAI TTAA 2 cut(s) 423, 474
SatI GCNGC 7 cut(s) 176, 238, 456, 498, 555, 569, 786
Sau3AI GATC 4 cut(s) 206, 232, 648, 793
Sau96I GGNCC 2 cut(s) 294, 728
SchI GAGTC 2 cut(s) 92, 296
ScrFI CCNGG 5 cut(s) 185, 292, 609, 810, 811
SduI GDGCHC 1 cut(s) 120
SexAI ACCWGGT 1 cut(s) 607
SfaNI GCATC 2 cut(s) 322, 340
SinI GGWCC 1 cut(s) 728
SmaI CCCGGG 1 cut(s) 811
SmiMI CAYNNNNRTG 1 cut(s) 680
SmlI CTYRAG 1 cut(s) 356
SmoI CTYRAG 1 cut(s) 356
Sse9I AATT 2 cut(s) 108, 447
SsiI CCGC 3 cut(s) 78, 237, 500
SspMI CTAG 1 cut(s) 252
StyD4I CCNGG 5 cut(s) 183, 290, 607, 808, 809
StyI CCWWGG 2 cut(s) 675, 826
TaaI ACNGT 2 cut(s) 57, 145
TaiI ACGT 1 cut(s) 841
TaqI TCGA 2 cut(s) 92, 857
TaqII GACCGA 1 cut(s) 578
TasI AATT 2 cut(s) 108, 447
TatI WGTACW 1 cut(s) 145
TauI GCSGC 1 cut(s) 240
TfiI GAWTC 2 cut(s) 25, 758
Tru1I TTAA 2 cut(s) 423, 474
Tru9I TTAA 2 cut(s) 423, 474
TscAI CASTG 2 cut(s) 637, 804
TseFI GTSAC 1 cut(s) 364
TseI GCWGC 6 cut(s) 175, 455, 497, 554, 568, 785
Tsp45I GTSAC 1 cut(s) 364
TspDTI ATGAA 1 cut(s) 659
TspMI CCCGGG 1 cut(s) 809
TspRI CASTG 2 cut(s) 637, 804
VpaK11BI GGWCC 1 cut(s) 728
XceI RCATGY 2 cut(s) 152, 215
XmaI CCCGGG 1 cut(s) 809
XspI CTAG 1 cut(s) 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.