FvH4_2g41440

protein At1g32220, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0012254
Physical Location & Seq
Reverse (-)
29310312 .. 29315156
4845 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g41440.t1

Sequence Viewer

Length: 870 bp
ATGAGGACGGTCATCTCGCGGTTGATCCATTCAAGATCATCACTTCCCAGGCCCTTTATCATGACTGCACCTAAAAATGGGAGGTATTTCTCAAATGACTTTAATAAGGTTGATGAGCCCTTCAAAGTTGAGGAAGCAGAGACTATTCAAGTCCCCCCTCCTCCAACAGAGAAGTTGCTTGTGTTGGGTGGAAATGGATTTGTTGGCTCACATGTTTGTAAAGAAGCATTAGATCGTGGACTGTCTGTTGCTAGCCTGAGCAGGTCTGGCAAGTCAAAGTTACATGATTCATGGGCCAACAATGTGACCTGGTATCAAGGGAACCTTCTATCAACTGATTCATGGAAGGATGCTCTCGACGGTGTCACATCTGTTATATCCTGTGTTGGTGGCTTTGGCTCCAATTCTTACATGTATAAGATTAATGGGACTGCAAACATCAATGCAGTTAGAGCAGCTTCTGAGCATGGTGTGAAAAGATTTGTGTATGTCTCTGCTGCTGATTTTGGCTTGGCAAATTACTTGGTGCGAGGATATTATGAGGGGAAGAGAGCTGCTGAAACAGAGATACTAACCAAATTCCCTTACGGAGGAGTGATTTTAAGGCCTGGATTTATTTACGGGACTCGAAGTGTTGGGAGCATAAAGTTACCTCTTGGTGTAATCGGTTCTCCGCTGGAGATGTTATTTCAACAAACAAGACCTCTCAACCAGCTGCCTCTAGTGGGCCCTCTCTTTACACCTCCTGTGAATGTTACGGCTGTAGCAAAGGTTGCTGTTAGAGCAGCTACTGATCCCGTCTTCCCTCCTGGGATTGTTGATATCTACGGGATTCAACGTTATACTCAGACTCAGCATAAGTCCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAD_binding_10

Protein Analysis

290

Amino Acids

31.51

Weight (kDa)

9.63

Isoelectric Point (pI)

32.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 56 - 165 1.3e-11 NmrA-like family
Epimerase PF01370 60 - 167 6.5e-14 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 60 - 175 1.3e-10 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_10 PF13460 63 - 206 1.9e-20 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012254)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10730
fragaria_vesca FvH4_2g41440 FvH4_2g41440
malus_domestica MD08G1049300.v1.1 MD15G1045600.v1.1
prunus_persica Prupe.1G399300_v2.0.a1
pyrus_communis pycom08g04120 pycom15g04250
rosa_chinensis RchiOBHm_Chr6g0299041
rosa_laevigata RLG00000011467
rosa_multiflora Rmu_sc0000981.1_g000004 Rmu_sc0032629.1_g000004
rosa_roxburghii Rroxscaffold_7G00169060
rosa_rugosa Rorug06G0284300
rosa_samantha Rh6AG395800 Rh6BG404400 Rh6CG410300 Rh6DG397000
rosa_wichuraiana Rw6G034640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 252
AccII CGCG 1 cut(s) 19
AciI CCGC 2 cut(s) 19, 674
AclI AACGTT 1 cut(s) 838
AclWI GGATC 2 cut(s) 19, 788
AcsI RAATTY 1 cut(s) 578
AfiI CCNNNNNNNGG 3 cut(s) 77, 590, 725
AflIII ACRYGT 2 cut(s) 211, 411
AgsI TTSAA 5 cut(s) 33, 124, 149, 692, 836
AjnI CCWGG 4 cut(s) 47, 308, 607, 808
AluBI AGCT 4 cut(s) 458, 554, 715, 788
AluI AGCT 4 cut(s) 458, 554, 715, 788
Alw26I GTCTC 2 cut(s) 134, 496
AlwI GGATC 2 cut(s) 19, 788
AlwNI CAGNNNCTG 2 cut(s) 461, 791
AoxI GGCC 4 cut(s) 50, 294, 605, 727
ApaI GGGCCC 1 cut(s) 731
ApeKI GCWGC 5 cut(s) 455, 497, 554, 715, 785
ApoI RAATTY 1 cut(s) 578
AseI ATTAAT 1 cut(s) 423
AspS9I GGNCC 4 cut(s) 51, 294, 727, 728
AsuNHI GCTAGC 1 cut(s) 251
BaeGI GKGCMC 1 cut(s) 731
BanII GRGCYC 2 cut(s) 120, 731
BbsI GAAGAC 1 cut(s) 793
BbvI GCAGC 5 cut(s) 467, 484, 541, 702, 797
BceAI ACGGC 1 cut(s) 774
BciT130I CCWGG 4 cut(s) 49, 310, 609, 810
BcoDI GTCTC 2 cut(s) 134, 496
BfaI CTAG 2 cut(s) 252, 722
BfmI CTRYAG 1 cut(s) 762
BfuAI ACCTGC 1 cut(s) 252
BisI GCNGC 5 cut(s) 456, 498, 555, 716, 786
BlsI GCNGC 5 cut(s) 457, 499, 556, 717, 787
Bme1390I CCNGG 4 cut(s) 49, 310, 609, 810
BmgT120I GGNCC 4 cut(s) 51, 294, 727, 728
BmiI GGNNCC 3 cut(s) 323, 400, 729
BmrFI CCNGG 4 cut(s) 49, 310, 609, 810
BmsI GCATC 1 cut(s) 340
BmtI GCTAGC 1 cut(s) 255
BpiI GAAGAC 1 cut(s) 793
BpmI CTGGAG 1 cut(s) 698
Bpu10I CCTNAGC 1 cut(s) 257
BsaJI CCNNGG 2 cut(s) 47, 809
Bsc4I CCNNNNNNNGG 3 cut(s) 77, 590, 725
BseBI CCWGG 4 cut(s) 49, 310, 609, 810
BseDI CCNNGG 2 cut(s) 47, 809
BseGI GGATG 1 cut(s) 355
BseLI CCNNNNNNNGG 3 cut(s) 77, 590, 725
BseMII CTCAG 4 cut(s) 248, 453, 860, 866
BseRI GAGGAG 2 cut(s) 150, 606
BseSI GKGCMC 1 cut(s) 731
BseXI GCAGC 5 cut(s) 467, 484, 541, 702, 797
BsgI GTGCAG 1 cut(s) 51
Bsh1236I CGCG 1 cut(s) 19
BshFI GGCC 4 cut(s) 52, 296, 607, 729
BslFI GGGAC 3 cut(s) 137, 442, 637
BslI CCNNNNNNNGG 3 cut(s) 77, 590, 725
BsmAI GTCTC 2 cut(s) 134, 496
BsmFI GGGAC 3 cut(s) 137, 442, 637
BsnI GGCC 4 cut(s) 52, 296, 607, 729
Bsp120I GGGCCC 1 cut(s) 727
Bsp1286I GDGCHC 2 cut(s) 120, 731
Bsp143I GATC 4 cut(s) 24, 35, 232, 793
BspACI CCGC 2 cut(s) 19, 674
BspANI GGCC 4 cut(s) 52, 296, 607, 729
BspCNI CTCAG 4 cut(s) 249, 454, 859, 865
BspFNI CGCG 1 cut(s) 19
BspHI TCATGA 1 cut(s) 60
BspLI GGNNCC 3 cut(s) 323, 400, 729
BspMI ACCTGC 1 cut(s) 252
BspOI GCTAGC 1 cut(s) 255
BspPI GGATC 2 cut(s) 19, 788
BssECI CCNNGG 2 cut(s) 47, 809
BssMI GATC 4 cut(s) 24, 35, 232, 793
Bst2UI CCWGG 4 cut(s) 49, 310, 609, 810
Bst4CI ACNGT 3 cut(s) 10, 243, 362
Bst6I CTCTTC 1 cut(s) 542
BstAPI GCANNNNNTGC 1 cut(s) 773
BstC8I GCNNGC 1 cut(s) 253
BstDEI CTNAG 4 cut(s) 257, 462, 846, 852
BstENI CCTNNNNNAGG 1 cut(s) 588
BstF5I GGATG 1 cut(s) 355
BstFNI CGCG 1 cut(s) 19
BstKTI GATC 4 cut(s) 27, 38, 235, 796
BstMAI GTCTC 2 cut(s) 134, 496
BstMBI GATC 4 cut(s) 24, 35, 232, 793
BstMWI GCNNNNNNNGC 4 cut(s) 267, 452, 773, 782
BstNI CCWGG 4 cut(s) 49, 310, 609, 810
BstNSI RCATGY 2 cut(s) 215, 415
BstSCI CCNGG 4 cut(s) 47, 308, 607, 808
BstSFI CTRYAG 1 cut(s) 762
BstSLI GKGCMC 1 cut(s) 731
BstUI CGCG 1 cut(s) 19
BstV1I GCAGC 5 cut(s) 467, 484, 541, 702, 797
BstV2I GAAGAC 1 cut(s) 793
BsuRI GGCC 4 cut(s) 52, 296, 607, 729
BtsCI GGATG 1 cut(s) 355
BveI ACCTGC 1 cut(s) 252
Cac8I GCNNGC 1 cut(s) 253
CaiI CAGNNNCTG 2 cut(s) 461, 791
CciI TCATGA 1 cut(s) 60
Cfr13I GGNCC 4 cut(s) 51, 294, 727, 728
CsiI ACCWGGT 1 cut(s) 308
CviAII CATG 7 cut(s) 61, 212, 284, 291, 342, 412, 467
DdeI CTNAG 4 cut(s) 257, 462, 846, 852
DpnI GATC 4 cut(s) 26, 37, 234, 795
DpnII GATC 4 cut(s) 24, 35, 232, 793
Eam1104I CTCTTC 1 cut(s) 542
EarI CTCTTC 1 cut(s) 542
Eco147I AGGCCT 1 cut(s) 607
Eco24I GRGCYC 2 cut(s) 120, 731
Eco32I GATATC 1 cut(s) 823
EcoNI CCTNNNNNAGG 1 cut(s) 588
EcoO109I RGGNCCY 2 cut(s) 51, 728
EcoRII CCWGG 4 cut(s) 47, 308, 607, 808
EcoRV GATATC 1 cut(s) 823
EcoT38I GRGCYC 2 cut(s) 120, 731
FaeI CATG 7 cut(s) 64, 215, 287, 294, 345, 415, 470
FalI AAGNNNNNCTT 2 cut(s) 309, 341
FaqI GGGAC 3 cut(s) 137, 442, 637
FatI CATG 7 cut(s) 60, 211, 283, 290, 341, 411, 466
Fnu4HI GCNGC 5 cut(s) 456, 498, 555, 716, 786
FokI GGATG 1 cut(s) 362
FriOI GRGCYC 2 cut(s) 120, 731
Fsp4HI GCNGC 5 cut(s) 456, 498, 555, 716, 786
FspBI CTAG 2 cut(s) 252, 722
GluI GCNGC 5 cut(s) 456, 498, 555, 716, 786
GsuI CTGGAG 1 cut(s) 698
HaeIII GGCC 4 cut(s) 52, 296, 607, 729
Hin1II CATG 7 cut(s) 64, 215, 287, 294, 345, 415, 470
HinfI GANTC 5 cut(s) 287, 338, 625, 832, 850
Hpy166II GTNNAC 1 cut(s) 239
Hpy188I TCNGA 2 cut(s) 463, 849
Hpy188III TCNNGA 3 cut(s) 33, 61, 356
Hpy8I GTNNAC 1 cut(s) 239
Hpy99I CGWCG 1 cut(s) 362
HpyAV CCTTC 3 cut(s) 130, 335, 340
HpyCH4III ACNGT 3 cut(s) 10, 243, 362
HpyCH4IV ACGT 1 cut(s) 838
HpyCH4V TGCA 3 cut(s) 68, 434, 446
HpyF10VI GCNNNNNNNGC 4 cut(s) 267, 452, 773, 782
HpyF3I CTNAG 4 cut(s) 257, 462, 846, 852
HpySE526I ACGT 1 cut(s) 838
Hsp92II CATG 7 cut(s) 64, 215, 287, 294, 345, 415, 470
Kzo9I GATC 4 cut(s) 24, 35, 232, 793
LmnI GCTCC 2 cut(s) 404, 639
Lsp1109I GCAGC 5 cut(s) 467, 484, 541, 702, 797
LweI GCATC 1 cut(s) 340
MabI ACCWGGT 1 cut(s) 308
MaeI CTAG 2 cut(s) 252, 722
MaeII ACGT 1 cut(s) 838
MaeIII GTNAC 5 cut(s) 279, 304, 364, 648, 754
MalI GATC 4 cut(s) 26, 37, 234, 795
MboI GATC 4 cut(s) 24, 35, 232, 793
MboII GAAGA 2 cut(s) 559, 793
MhlI GDGCHC 2 cut(s) 120, 731
MluCI AATT 3 cut(s) 403, 517, 578
MlyI GAGTC 2 cut(s) 619, 844
MmeI TCCRAC 1 cut(s) 188
MseI TTAA 3 cut(s) 102, 423, 602
MspA1I CMGCKG 2 cut(s) 676, 715
MspR9I CCNGG 4 cut(s) 49, 310, 609, 810
MvaI CCWGG 4 cut(s) 49, 310, 609, 810
MvnI CGCG 1 cut(s) 19
MwoI GCNNNNNNNGC 4 cut(s) 267, 452, 773, 782
NdeII GATC 4 cut(s) 24, 35, 232, 793
NheI GCTAGC 1 cut(s) 251
NlaIII CATG 7 cut(s) 64, 215, 287, 294, 345, 415, 470
NlaIV GGNNCC 3 cut(s) 323, 400, 729
NmuCI GTSAC 2 cut(s) 304, 364
NspI RCATGY 2 cut(s) 215, 415
PagI TCATGA 1 cut(s) 60
PceI AGGCCT 1 cut(s) 607
PciI ACATGT 2 cut(s) 211, 411
PfeI GAWTC 3 cut(s) 287, 338, 832
PflFI GACNNNGTC 1 cut(s) 362
PkrI GCNGC 5 cut(s) 457, 499, 556, 717, 787
PleI GAGTC 2 cut(s) 619, 844
PpsI GAGTC 2 cut(s) 619, 844
PscI ACATGT 2 cut(s) 211, 411
PshBI ATTAAT 1 cut(s) 423
Psp1406I AACGTT 1 cut(s) 838
Psp6I CCWGG 4 cut(s) 47, 308, 607, 808
PspGI CCWGG 4 cut(s) 47, 308, 607, 808
PspN4I GGNNCC 3 cut(s) 323, 400, 729
PspOMI GGGCCC 1 cut(s) 727
PspPI GGNCC 4 cut(s) 51, 294, 727, 728
PstNI CAGNNNCTG 2 cut(s) 461, 791
PsyI GACNNNGTC 1 cut(s) 362
PvuII CAGCTG 1 cut(s) 715
SaqAI TTAA 3 cut(s) 102, 423, 602
SatI GCNGC 5 cut(s) 456, 498, 555, 716, 786
Sau3AI GATC 4 cut(s) 24, 35, 232, 793
Sau96I GGNCC 4 cut(s) 51, 294, 727, 728
SchI GAGTC 2 cut(s) 619, 844
ScrFI CCNGG 4 cut(s) 49, 310, 609, 810
SduI GDGCHC 2 cut(s) 120, 731
SexAI ACCWGGT 1 cut(s) 308
SfaNI GCATC 1 cut(s) 340
SfcI CTRYAG 1 cut(s) 762
Sse9I AATT 3 cut(s) 403, 517, 578
SseBI AGGCCT 1 cut(s) 607
SsiI CCGC 2 cut(s) 19, 674
SspMI CTAG 2 cut(s) 252, 722
StuI AGGCCT 1 cut(s) 607
StyD4I CCNGG 4 cut(s) 47, 308, 607, 808
TaaI ACNGT 3 cut(s) 10, 243, 362
TaiI ACGT 1 cut(s) 841
TaqI TCGA 2 cut(s) 357, 628
TasI AATT 3 cut(s) 403, 517, 578
TfiI GAWTC 3 cut(s) 287, 338, 832
Tru1I TTAA 3 cut(s) 102, 423, 602
Tru9I TTAA 3 cut(s) 102, 423, 602
TseFI GTSAC 2 cut(s) 304, 364
TseI GCWGC 5 cut(s) 455, 497, 554, 715, 785
Tsp45I GTSAC 2 cut(s) 304, 364
TspDTI ATGAA 2 cut(s) 279, 330
TspGWI ACGGA 1 cut(s) 603
Tth111I GACNNNGTC 1 cut(s) 362
VspI ATTAAT 1 cut(s) 423
XagI CCTNNNNNAGG 1 cut(s) 588
XapI RAATTY 1 cut(s) 578
XceI RCATGY 2 cut(s) 215, 415
XspI CTAG 2 cut(s) 252, 722
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.