MD15G1045600.v1.1

protein At1g32220, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0012254
Physical Location & Seq
Forward (+)
3137479 .. 3140888
3410 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1045600.v1.1.491

Sequence Viewer

Length: 861 bp
ATGAGGACGGTCGTGTCGCGGTTGATCCAATCAAGATCATCACTTCCAAGGCTCTTCAGTGTATCGGAATCACTGAAGAATGGTAGGTATCTAACAACAGACTCTAATAAGGTTGATGAACCCTTCAAAGTTGAAGAAGCTGAGACTGTCAACATTCCCCCTCCTCCAACTGATAAGTTGCTTGTCTTGGGTGGAAATGGATTTGTTGGCTCACATGTTTGTAGAGAAGCATTGGATCGGGGCTTGTCTGTCGCTAGCCTTAGCAGGTCTGGTAGGTCAAAATTACATGATTTGTGGGCCAACAGTGTGACCTGGCATCAAGGAAACCTTCTATCGCCTGAGTCATTAAAGGATGCTTTCAATGGTGTCACGTCCGTCATATCCTGTGTTGGTGGGTTTGGCTCCAATTCTTACATGTATAAGATTAATGGGACTGCTAACATCAATGCAATTAGAGTTGCCGCTGAACAAGGCGTGAAAAGATTTGTTTATATCTCTGCTGCTGATTTTGGTGTGGCAAATTACTTACTGCAGGGATATTATGAGGGAAAGAGAGCTGCAGAGACAGAGCTACTAACCAAATTCCCATATGGAGGAGTGATTTTGAGGCCTGGATTTATATATGGGACTCGAAGTGTTGGGAGCCTGAAGATACCTCTTGGTGTAATTGGTTCTCCACTTGAGATGCTATTTCAAAACACGAGACCCCTGAGCCAGCTGCCGCTTGTTGGGCCTCTCTTTACTCCTCCAGTGAATGTAACCTCTGTGGCAAATGTTGCCGTTAGAGCAGCTACTGATCCTGTCTTCCCTCCTGGGATTGTAGATGTCCAGGGTATCCAGCGTTATACCCAGAAGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAD_binding_10

Protein Analysis

287

Amino Acids

30.93

Weight (kDa)

9.58

Isoelectric Point (pI)

38.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 57 - 166 1.8e-11 NmrA-like family
Epimerase PF01370 61 - 168 1.4e-14 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 61 - 189 4.7e-10 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_10 PF13460 64 - 207 1.2e-19 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012254)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10730
fragaria_vesca FvH4_2g41440 FvH4_2g41440
malus_domestica MD08G1049300.v1.1 MD15G1045600.v1.1
prunus_persica Prupe.1G399300_v2.0.a1
pyrus_communis pycom08g04120 pycom15g04250
rosa_chinensis RchiOBHm_Chr6g0299041
rosa_laevigata RLG00000011467
rosa_multiflora Rmu_sc0000981.1_g000004 Rmu_sc0032629.1_g000004
rosa_roxburghii Rroxscaffold_7G00169060
rosa_rugosa Rorug06G0284300
rosa_samantha Rh6AG395800 Rh6BG404400 Rh6CG410300 Rh6DG397000
rosa_wichuraiana Rw6G034640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 13
Acc36I ACCTGC 1 cut(s) 255
AccII CGCG 1 cut(s) 19
AciI CCGC 3 cut(s) 19, 462, 722
AclWI GGATC 3 cut(s) 19, 243, 791
AcsI RAATTY 1 cut(s) 581
AcuI CTGAAG 3 cut(s) 40, 95, 668
AfiI CCNNNNNNNGG 2 cut(s) 593, 728
AflIII ACRYGT 2 cut(s) 214, 414
AgsI TTSAA 4 cut(s) 127, 134, 361, 695
AjiI CACGTC 1 cut(s) 372
AjnI CCWGG 4 cut(s) 311, 610, 811, 828
AluBI AGCT 5 cut(s) 140, 557, 571, 718, 791
AluI AGCT 5 cut(s) 140, 557, 571, 718, 791
Alw26I GTCTC 3 cut(s) 137, 557, 697
AlwI GGATC 3 cut(s) 19, 243, 791
AlwNI CAGNNNCTG 1 cut(s) 794
AoxI GGCC 3 cut(s) 297, 608, 731
ApeKI GCWGC 4 cut(s) 500, 557, 718, 788
ApoI RAATTY 1 cut(s) 581
AseI ATTAAT 1 cut(s) 426
AspS9I GGNCC 2 cut(s) 297, 731
AsuNHI GCTAGC 1 cut(s) 254
BauI CACGAG 1 cut(s) 700
BbsI GAAGAC 1 cut(s) 796
BbvI GCAGC 4 cut(s) 487, 544, 705, 800
BceAI ACGGC 1 cut(s) 764
BciT130I CCWGG 4 cut(s) 313, 612, 813, 830
BciVI GTATCC 1 cut(s) 845
BcoDI GTCTC 3 cut(s) 137, 557, 697
BfaI CTAG 1 cut(s) 255
BfmI CTRYAG 2 cut(s) 530, 558
BfuAI ACCTGC 1 cut(s) 255
BfuI GTATCC 1 cut(s) 845
BisI GCNGC 6 cut(s) 462, 501, 558, 719, 722, 789
BlsI GCNGC 6 cut(s) 463, 502, 559, 720, 723, 790
Bme1390I CCNGG 4 cut(s) 313, 612, 813, 830
BmgBI CACGTC 1 cut(s) 372
BmgT120I GGNCC 2 cut(s) 297, 731
BmiI GGNNCC 2 cut(s) 403, 644
BmrFI CCNGG 4 cut(s) 313, 612, 813, 830
BmsI GCATC 3 cut(s) 325, 343, 675
BmtI GCTAGC 1 cut(s) 258
BpiI GAAGAC 1 cut(s) 796
BpmI CTGGAG 1 cut(s) 732
Bpu10I CCTNAGC 2 cut(s) 260, 710
BpuEI CTTGAG 1 cut(s) 701
BsaI GGTCTC 1 cut(s) 697
BsaJI CCNNGG 3 cut(s) 47, 812, 829
Bsc4I CCNNNNNNNGG 2 cut(s) 593, 728
Bse1I ACTGG 1 cut(s) 749
BseBI CCWGG 4 cut(s) 313, 612, 813, 830
BseDI CCNNGG 3 cut(s) 47, 812, 829
BseGI GGATG 1 cut(s) 358
BseLI CCNNNNNNNGG 2 cut(s) 593, 728
BseMII CTCAG 3 cut(s) 132, 330, 701
BseNI ACTGG 1 cut(s) 749
BseRI GAGGAG 3 cut(s) 153, 609, 735
BseXI GCAGC 4 cut(s) 487, 544, 705, 800
Bsh1236I CGCG 1 cut(s) 19
Bsh1285I CGRYCG 1 cut(s) 12
BshFI GGCC 3 cut(s) 299, 610, 733
BsiEI CGRYCG 1 cut(s) 12
BslFI GGGAC 2 cut(s) 445, 640
BslI CCNNNNNNNGG 2 cut(s) 593, 728
BsmAI GTCTC 3 cut(s) 137, 557, 697
BsmFI GGGAC 2 cut(s) 445, 640
BsnI GGCC 3 cut(s) 299, 610, 733
Bso31I GGTCTC 1 cut(s) 697
Bsp143I GATC 4 cut(s) 24, 35, 235, 796
BspACI CCGC 3 cut(s) 19, 462, 722
BspANI GGCC 3 cut(s) 299, 610, 733
BspCNI CTCAG 3 cut(s) 133, 331, 702
BspFNI CGCG 1 cut(s) 19
BspLI GGNNCC 2 cut(s) 403, 644
BspMAI CTGCAG 2 cut(s) 534, 562
BspMI ACCTGC 1 cut(s) 255
BspOI GCTAGC 1 cut(s) 258
BspPI GGATC 3 cut(s) 19, 243, 791
BspQI GCTCTTC 1 cut(s) 59
BspTNI GGTCTC 1 cut(s) 697
BsrI ACTGG 1 cut(s) 749
BssECI CCNNGG 3 cut(s) 47, 812, 829
BssMI GATC 4 cut(s) 24, 35, 235, 796
BssSI CACGAG 1 cut(s) 700
BssT1I CCWWGG 1 cut(s) 47
Bst2BI CACGAG 1 cut(s) 700
Bst2UI CCWGG 4 cut(s) 313, 612, 813, 830
Bst4CI ACNGT 3 cut(s) 10, 148, 305
Bst6I CTCTTC 1 cut(s) 59
BstAPI GCANNNNNTGC 1 cut(s) 776
BstC8I GCNNGC 2 cut(s) 256, 716
BstDEI CTNAG 4 cut(s) 141, 260, 339, 710
BstF5I GGATG 1 cut(s) 358
BstFNI CGCG 1 cut(s) 19
BstKTI GATC 4 cut(s) 27, 38, 238, 799
BstMAI GTCTC 3 cut(s) 137, 557, 697
BstMBI GATC 4 cut(s) 24, 35, 235, 796
BstMCI CGRYCG 1 cut(s) 12
BstMWI GCNNNNNNNGC 3 cut(s) 730, 776, 785
BstNI CCWGG 4 cut(s) 313, 612, 813, 830
BstNSI RCATGY 2 cut(s) 218, 418
BstSCI CCNGG 4 cut(s) 311, 610, 811, 828
BstSFI CTRYAG 2 cut(s) 530, 558
BstUI CGCG 1 cut(s) 19
BstV1I GCAGC 4 cut(s) 487, 544, 705, 800
BstV2I GAAGAC 1 cut(s) 796
BsuI GTATCC 1 cut(s) 845
BsuRI GGCC 3 cut(s) 299, 610, 733
BtrI CACGTC 1 cut(s) 372
BtsCI GGATG 1 cut(s) 358
BtsIMutI CAGTG 4 cut(s) 64, 71, 310, 756
BveI ACCTGC 1 cut(s) 255
Cac8I GCNNGC 2 cut(s) 256, 716
CaiI CAGNNNCTG 1 cut(s) 794
Cfr13I GGNCC 2 cut(s) 297, 731
CviAII CATG 3 cut(s) 215, 287, 415
DdeI CTNAG 4 cut(s) 141, 260, 339, 710
DpnI GATC 4 cut(s) 26, 37, 237, 798
DpnII GATC 4 cut(s) 24, 35, 235, 796
DrdI GACNNNNNNGTC 1 cut(s) 13
DseDI GACNNNNNNGTC 1 cut(s) 13
Eam1104I CTCTTC 1 cut(s) 59
EarI CTCTTC 1 cut(s) 59
Eco130I CCWWGG 1 cut(s) 47
Eco147I AGGCCT 1 cut(s) 610
Eco31I GGTCTC 1 cut(s) 697
Eco57I CTGAAG 3 cut(s) 40, 95, 668
EcoRII CCWGG 4 cut(s) 311, 610, 811, 828
EcoT14I CCWWGG 1 cut(s) 47
ErhI CCWWGG 1 cut(s) 47
FaeI CATG 3 cut(s) 218, 290, 418
FalI AAGNNNNNCTT 2 cut(s) 312, 344
FaqI GGGAC 2 cut(s) 445, 640
FatI CATG 3 cut(s) 214, 286, 414
FauNDI CATATG 1 cut(s) 589
Fnu4HI GCNGC 6 cut(s) 462, 501, 558, 719, 722, 789
FokI GGATG 1 cut(s) 365
Fsp4HI GCNGC 6 cut(s) 462, 501, 558, 719, 722, 789
FspBI CTAG 1 cut(s) 255
GluI GCNGC 6 cut(s) 462, 501, 558, 719, 722, 789
GsuI CTGGAG 1 cut(s) 732
HaeIII GGCC 3 cut(s) 299, 610, 733
Hin1II CATG 3 cut(s) 218, 290, 418
HincII GTYRAC 1 cut(s) 151
HindII GTYRAC 1 cut(s) 151
HinfI GANTC 4 cut(s) 68, 101, 341, 628
Hpy166II GTNNAC 1 cut(s) 151
Hpy188I TCNGA 1 cut(s) 67
Hpy188III TCNNGA 1 cut(s) 33
Hpy8I GTNNAC 1 cut(s) 151
HpyAV CCTTC 2 cut(s) 133, 338
HpyCH4III ACNGT 3 cut(s) 10, 148, 305
HpyCH4IV ACGT 1 cut(s) 371
HpyCH4V TGCA 3 cut(s) 449, 532, 560
HpyF10VI GCNNNNNNNGC 3 cut(s) 730, 776, 785
HpyF3I CTNAG 4 cut(s) 141, 260, 339, 710
HpySE526I ACGT 1 cut(s) 371
Hsp92II CATG 3 cut(s) 218, 290, 418
Kzo9I GATC 4 cut(s) 24, 35, 235, 796
LguI GCTCTTC 1 cut(s) 59
LmnI GCTCC 2 cut(s) 407, 642
Lsp1109I GCAGC 4 cut(s) 487, 544, 705, 800
LweI GCATC 3 cut(s) 325, 343, 675
MaeI CTAG 1 cut(s) 255
MaeII ACGT 1 cut(s) 371
MaeIII GTNAC 3 cut(s) 307, 367, 757
MalI GATC 4 cut(s) 26, 37, 237, 798
MboI GATC 4 cut(s) 24, 35, 235, 796
MboII GAAGA 5 cut(s) 46, 88, 146, 661, 796
MluCI AATT 6 cut(s) 281, 406, 450, 520, 581, 666
MlyI GAGTC 3 cut(s) 95, 350, 622
MmeI TCCRAC 1 cut(s) 191
MseI TTAA 2 cut(s) 347, 426
MspA1I CMGCKG 2 cut(s) 464, 718
MspR9I CCNGG 4 cut(s) 313, 612, 813, 830
MvaI CCWGG 4 cut(s) 313, 612, 813, 830
MvnI CGCG 1 cut(s) 19
MwoI GCNNNNNNNGC 3 cut(s) 730, 776, 785
NdeI CATATG 1 cut(s) 589
NdeII GATC 4 cut(s) 24, 35, 235, 796
NheI GCTAGC 1 cut(s) 254
NlaIII CATG 3 cut(s) 218, 290, 418
NlaIV GGNNCC 2 cut(s) 403, 644
NmuCI GTSAC 2 cut(s) 307, 367
NspI RCATGY 2 cut(s) 218, 418
PceI AGGCCT 1 cut(s) 610
PciI ACATGT 2 cut(s) 214, 414
PciSI GCTCTTC 1 cut(s) 59
PfeI GAWTC 1 cut(s) 68
PkrI GCNGC 6 cut(s) 463, 502, 559, 720, 723, 790
PleI GAGTC 3 cut(s) 95, 349, 622
PpsI GAGTC 3 cut(s) 95, 349, 622
PscI ACATGT 2 cut(s) 214, 414
PshBI ATTAAT 1 cut(s) 426
Psp6I CCWGG 4 cut(s) 311, 610, 811, 828
PspGI CCWGG 4 cut(s) 311, 610, 811, 828
PspN4I GGNNCC 2 cut(s) 403, 644
PspPI GGNCC 2 cut(s) 297, 731
PstI CTGCAG 2 cut(s) 534, 562
PstNI CAGNNNCTG 1 cut(s) 794
PvuII CAGCTG 1 cut(s) 718
SapI GCTCTTC 1 cut(s) 59
SaqAI TTAA 2 cut(s) 347, 426
SatI GCNGC 6 cut(s) 462, 501, 558, 719, 722, 789
Sau3AI GATC 4 cut(s) 24, 35, 235, 796
Sau96I GGNCC 2 cut(s) 297, 731
SchI GAGTC 3 cut(s) 95, 350, 622
ScrFI CCNGG 4 cut(s) 313, 612, 813, 830
SfaNI GCATC 3 cut(s) 325, 343, 675
SfcI CTRYAG 2 cut(s) 530, 558
SmlI CTYRAG 1 cut(s) 680
SmoI CTYRAG 1 cut(s) 680
Sse9I AATT 6 cut(s) 281, 406, 450, 520, 581, 666
SseBI AGGCCT 1 cut(s) 610
SsiI CCGC 3 cut(s) 19, 462, 722
SspMI CTAG 1 cut(s) 255
StuI AGGCCT 1 cut(s) 610
StyD4I CCNGG 4 cut(s) 311, 610, 811, 828
StyI CCWWGG 1 cut(s) 47
TaaI ACNGT 3 cut(s) 10, 148, 305
TaiI ACGT 1 cut(s) 374
TaqI TCGA 1 cut(s) 631
TasI AATT 6 cut(s) 281, 406, 450, 520, 581, 666
TauI GCSGC 2 cut(s) 464, 724
TfiI GAWTC 1 cut(s) 68
Tru1I TTAA 2 cut(s) 347, 426
Tru9I TTAA 2 cut(s) 347, 426
TscAI CASTG 4 cut(s) 64, 78, 310, 756
TseFI GTSAC 2 cut(s) 307, 367
TseI GCWGC 4 cut(s) 500, 557, 718, 788
Tsp45I GTSAC 2 cut(s) 307, 367
TspDTI ATGAA 1 cut(s) 132
TspGWI ACGGA 1 cut(s) 364
TspRI CASTG 4 cut(s) 64, 78, 310, 756
VspI ATTAAT 1 cut(s) 426
XapI RAATTY 1 cut(s) 581
XceI RCATGY 2 cut(s) 218, 418
XspI CTAG 1 cut(s) 255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.